RchiOBHm_Chr1g0313191

Clp protease-related protein At4g12060

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012795
Physical Location & Seq
Forward (+)
312488 .. 315491
3004 bp
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UTR
Exon/CDS
Intron
PRQ54401

Sequence Viewer

Length: 699 bp
ATGACCTCCGTTTCCATAACACTGTCAGCTCTCTCAGTCTCACTCTCTACCACCGAACCACCTTCCCTTGGAAACCCTAGCAATTCAACACCCTGCAGCCTATCTACCTCATTCCTCGGCAGAAAGCTCTCCGTTGGAGTTCCACATTCAAACAAGTTGGCTTTAAAACATCCAACAGCTGTCGCCACCGTTCTCTTCAGCCTCCCAACAGGGAAACCAGAGAGGATTTCTTCTGGGAAAAGCTCACAATGGTCGGCCAGAGCGATTAAGTCGTTTGCTATGGGTGAATTGGAAGCAAGGAAGCTCAAGTATCCCAATACTGGCACGGAAGCCCTTCTAATGGGAATTTTAGTCGAGGGAACAAGTATAGCTGCGAAGTTTCTCAGAGCTAATGGAATCACGCTTTTCAAGGTTCGAGAAGAAACTGTAAAGTTACTTGGAAAGTCAGACATGTACTTTTTCAGCCCCGAGCATCCTCCATTGACCGAGCCAGCTCAGAGGGCCCTTGACTGGGCTGTGGATCAAAAACTGAAGTCAGGAGACAGTGGGGAAATAACAGTAACTCATCTGCTTCTCGGGATTTGGTCTGAAAAAGAGTCAGCAGGTCACAAGATCTTGGTGTCCCTTGGTTTTGATGATGAGAAAGCAAAAGAGCTTTCTGTATCTATGGACAAGGATTATGTTCCTAGCTATAAATAA

Protein Analysis

232

Amino Acids

24.99

Weight (kDa)

9.06

Isoelectric Point (pI)

24.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 87 - 207 9.1e-15 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25370
fragaria_vesca FvH4_7g00050
malus_domestica MD00G1055600.v1.1 MD02G1320000.v1.1
prunus_persica Prupe.2G002100_v2.0.a1
pyrus_communis pycom07g00100
rosa_chinensis RchiOBHm_Chr1g0313031 RchiOBHm_Chr1g0313191
rosa_laevigata RLG00000030817
rosa_multiflora Rmu_co8509777.1_g000001
rosa_roxburghii Rroxscaffold_4G00332400
rosa_rugosa RorugPtG0001300.1
rosa_samantha Rh1AG000400 Rh1AG001500 Rh1CG003500 Rh1CG006100 Rh1DG001400
rosa_wichuraiana Rw1G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 593
AclWI GGATC 1 cut(s) 528
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 2 cut(s) 181, 551
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 5 cut(s) 68, 320, 340, 510, 511
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 3 cut(s) 87, 150, 409
Alw26I GTCTC 2 cut(s) 43, 534
AlwI GGATC 1 cut(s) 528
Ama87I CYCGRG 2 cut(s) 467, 575
AoxI GGCC 2 cut(s) 255, 501
ApaI GGGCCC 1 cut(s) 505
ApeKI GCWGC 2 cut(s) 96, 371
ApoI RAATTY 1 cut(s) 345
Asp700I GAANNNNTTC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 501, 502
AsuHPI GGTGA 1 cut(s) 296
AvaI CYCGRG 2 cut(s) 467, 575
BaeGI GKGCMC 1 cut(s) 505
BanII GRGCYC 1 cut(s) 505
BbvI GCAGC 2 cut(s) 108, 358
BciVI GTATCC 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 43, 534
BfaI CTAG 2 cut(s) 78, 687
BfmI CTRYAG 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 593
BfuI GTATCC 1 cut(s) 321
BglII AGATCT 1 cut(s) 612
BisI GCNGC 2 cut(s) 97, 372
BlsI GCNGC 2 cut(s) 98, 373
BmeT110I CYCGRG 2 cut(s) 467, 575
BmgT120I GGNCC 2 cut(s) 501, 502
BmiI GGNNCC 1 cut(s) 503
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 520
BpuEI CTTGAG 1 cut(s) 290
BsaJI CCNNGG 3 cut(s) 67, 115, 625
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 320, 340, 510, 511
Bse1I ACTGG 2 cut(s) 325, 515
BseDI CCNNGG 3 cut(s) 67, 115, 625
BseGI GGATG 2 cut(s) 169, 472
BseLI CCNNNNNNNGG 5 cut(s) 68, 320, 340, 510, 511
BseMII CTCAG 3 cut(s) 48, 397, 509
BseNI ACTGG 2 cut(s) 325, 515
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 2 cut(s) 108, 358
BshFI GGCC 2 cut(s) 257, 503
BsiHKCI CYCGRG 2 cut(s) 467, 575
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 5 cut(s) 68, 320, 340, 510, 511
BsmAI GTCTC 2 cut(s) 43, 534
BsmFI GGGAC 1 cut(s) 607
BsnI GGCC 2 cut(s) 257, 503
BsoBI CYCGRG 2 cut(s) 467, 575
Bsp120I GGGCCC 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 2 cut(s) 520, 612
BspANI GGCC 2 cut(s) 257, 503
BspCNI CTCAG 3 cut(s) 47, 396, 508
BspLI GGNNCC 1 cut(s) 503
BspMAI CTGCAG 1 cut(s) 98
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 1 cut(s) 528
BsrI ACTGG 2 cut(s) 325, 515
BssECI CCNNGG 3 cut(s) 67, 115, 625
BssMI GATC 2 cut(s) 520, 612
BssT1I CCWWGG 2 cut(s) 67, 625
Bst4CI ACNGT 5 cut(s) 24, 190, 427, 545, 559
Bst6I CTCTTC 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 3 cut(s) 34, 383, 495
BstF5I GGATG 2 cut(s) 169, 472
BstKTI GATC 2 cut(s) 523, 615
BstMAI GTCTC 2 cut(s) 43, 534
BstMBI GATC 2 cut(s) 520, 612
BstMWI GCNNNNNNNGC 1 cut(s) 500
BstNSI RCATGY 1 cut(s) 454
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 505
BstV1I GCAGC 2 cut(s) 108, 358
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
BsuI GTATCC 1 cut(s) 321
BsuRI GGCC 2 cut(s) 257, 503
BtsCI GGATG 2 cut(s) 169, 472
BtsIMutI CAGTG 2 cut(s) 20, 550
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 1 cut(s) 492
Cfr13I GGNCC 2 cut(s) 501, 502
Csp6I GTAC 1 cut(s) 454
CviAII CATG 1 cut(s) 451
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 34, 383, 495
DpnI GATC 2 cut(s) 522, 614
DpnII GATC 2 cut(s) 520, 612
DraI TTTAAA 1 cut(s) 165
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 2 cut(s) 67, 625
Eco24I GRGCYC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 181, 551
Eco88I CYCGRG 2 cut(s) 467, 575
EcoO109I RGGNCCY 2 cut(s) 501, 502
EcoT14I CCWWGG 2 cut(s) 67, 625
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 2 cut(s) 67, 625
FaeI CATG 1 cut(s) 454
FaiI YATR 7 cut(s) 17, 281, 368, 452, 668, 681, 693
FaqI GGGAC 1 cut(s) 607
FatI CATG 1 cut(s) 450
Fnu4HI GCNGC 2 cut(s) 97, 372
FokI GGATG 2 cut(s) 156, 459
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 2 cut(s) 97, 372
FspBI CTAG 2 cut(s) 78, 687
GluI GCNGC 2 cut(s) 97, 372
HaeIII GGCC 2 cut(s) 257, 503
Hin1II CATG 1 cut(s) 454
HinfI GANTC 2 cut(s) 396, 596
HphI GGTGA 1 cut(s) 296
Hpy188I TCNGA 4 cut(s) 386, 448, 498, 589
Hpy188III TCNNGA 3 cut(s) 416, 537, 577
HpyAV CCTTC 2 cut(s) 72, 344
HpyCH4III ACNGT 5 cut(s) 24, 190, 427, 545, 559
HpyCH4V TGCA 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 1 cut(s) 500
HpyF3I CTNAG 3 cut(s) 34, 383, 495
Hsp92II CATG 1 cut(s) 454
Kzo9I GATC 2 cut(s) 520, 612
Lsp1109I GCAGC 2 cut(s) 108, 358
LweI GCATC 1 cut(s) 481
MaeI CTAG 2 cut(s) 78, 687
MaeIII GTNAC 3 cut(s) 432, 559, 605
MalI GATC 2 cut(s) 522, 614
MboI GATC 2 cut(s) 520, 612
MboII GAAGA 3 cut(s) 187, 222, 431
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 3 cut(s) 82, 287, 345
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 2 cut(s) 115, 197
MnlI CCTC 8 cut(s) 16, 118, 125, 212, 216, 349, 486, 492
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 2 cut(s) 164, 267
MspA1I CMGCKG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 500
NdeII GATC 2 cut(s) 520, 612
NlaIII CATG 1 cut(s) 454
NlaIV GGNNCC 1 cut(s) 503
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 605
NspI RCATGY 1 cut(s) 454
PciI ACATGT 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 396
PkrI GCNGC 2 cut(s) 98, 373
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
PscI ACATGT 1 cut(s) 450
PspN4I GGNNCC 1 cut(s) 503
PspOMI GGGCCC 1 cut(s) 501
PspPI GGNCC 2 cut(s) 501, 502
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 1 cut(s) 612
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 164, 267
SatI GCNGC 2 cut(s) 97, 372
Sau3AI GATC 2 cut(s) 520, 612
Sau96I GGNCC 2 cut(s) 501, 502
SchI GAGTC 1 cut(s) 605
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 94
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 3 cut(s) 82, 287, 345
SspMI CTAG 2 cut(s) 78, 687
StyI CCWWGG 2 cut(s) 67, 625
TaaI ACNGT 5 cut(s) 24, 190, 427, 545, 559
TaqI TCGA 2 cut(s) 354, 415
TaqII GACCGA 1 cut(s) 500
TasI AATT 3 cut(s) 82, 287, 345
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 2 cut(s) 164, 267
Tru9I TTAA 2 cut(s) 164, 267
TscAI CASTG 2 cut(s) 27, 550
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 2 cut(s) 96, 371
Tsp45I GTSAC 1 cut(s) 605
TspGWI ACGGA 2 cut(s) 121, 341
TspRI CASTG 2 cut(s) 27, 550
XapI RAATTY 1 cut(s) 345
XceI RCATGY 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 2 cut(s) 78, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.