MD02G1320000.v1.1

Clp protease-related protein At4g12060

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012795
Physical Location & Seq
Reverse (-)
37424887 .. 37427502
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1320000.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGCATCCACCTCCTTTGCACTCTCAGCTCTCTCAATATCACCCTCTTCTACCTCTCAACCCCACAGAAACCCTAGCGCCTACTCTTCTCCCTCCCTTCCCCGCCATCTTCCCCCCTACAACCTATCTACCACTTTTCTCGGCTCAAGGCTTTCCATTCGAATTCCGAATCTAAAGCACATGGCTTCCAAGCCTCGGACGGCCGTCGCCACCGTCCTCTTCAGCCTCCCAACAGCGAAGCCCGACAGGATTTCTACTGGGAAAAACTCCAAATGGTCCGCCAGAGCGATAAAGTCGTTTGCTATGGCTGAATTGGAAGCAAGGAAGCTTAAATATCCCAATACAGGGACCGAAGCACTTCTCATGGGGATTTTGGTTGAGGGAACGAGCCTAGCTGCAAAGTTTCTTAGAGCTAATGGAATTACGCTCTTCAAGGTTCGAGATGAAACTGTAAATTTACTTGGAAAATCAGACATGTACTATTTCAGCCCTGAGCATCCTCCATTAACTGAACCAGCTCAGAGGGCCCTTGACTGGGCTTTTGATCAGAAACTAAAATCAGGAGAAAATGGGGAAATAACAGTAACTCATCTGCTTCTTGGGATTTGGTCTGAAAAAGAGTCAGCAGGTCACAAGATCTTGGCGTCCCTAGGTTTTGATGATGATAAAGCTACAGAGCTTTCCAAATTTATGGATAAGGATTATGATCGTAGCTTTAAATAA

Protein Analysis

241

Amino Acids

26.42

Weight (kDa)

9.59

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 92 - 215 1.4e-14 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25370
fragaria_vesca FvH4_7g00050
malus_domestica MD00G1055600.v1.1 MD02G1320000.v1.1
prunus_persica Prupe.2G002100_v2.0.a1
pyrus_communis pycom07g00100
rosa_chinensis RchiOBHm_Chr1g0313031 RchiOBHm_Chr1g0313191
rosa_laevigata RLG00000030817
rosa_multiflora Rmu_co8509777.1_g000001
rosa_roxburghii Rroxscaffold_4G00332400
rosa_rugosa RorugPtG0001300.1
rosa_samantha Rh1AG000400 Rh1AG001500 Rh1CG003500 Rh1CG006100 Rh1DG001400
rosa_wichuraiana Rw1G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 617
AciI CCGC 2 cut(s) 103, 279
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 3 cut(s) 162, 454, 686
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 5 cut(s) 195, 344, 345, 534, 535
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 1 cut(s) 433
AluBI AGCT 8 cut(s) 29, 328, 395, 413, 518, 671, 679, 714
AluI AGCT 8 cut(s) 29, 328, 395, 413, 518, 671, 679, 714
AoxI GGCC 2 cut(s) 201, 525
ApaI GGGCCC 1 cut(s) 529
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 3 cut(s) 162, 454, 686
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
Asp700I GAANNNNTTC 1 cut(s) 357
AspA2I CCTAGG 1 cut(s) 649
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 4 cut(s) 276, 348, 525, 526
AsuHPI GGTGA 1 cut(s) 33
AsuII TTCGAA 1 cut(s) 160
AvaII GGWCC 2 cut(s) 276, 348
AvrII CCTAGG 1 cut(s) 649
BaeGI GKGCMC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 114
BceAI ACGGC 2 cut(s) 188, 216
BclI TGATCA 1 cut(s) 544
BfaI CTAG 3 cut(s) 75, 392, 650
BfmI CTRYAG 1 cut(s) 672
BfoI RGCGCY 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 617
BglII AGATCT 1 cut(s) 636
BisI GCNGC 1 cut(s) 396
BlnI CCTAGG 1 cut(s) 649
BlsI GCNGC 1 cut(s) 397
Bme18I GGWCC 2 cut(s) 276, 348
BmgT120I GGNCC 4 cut(s) 276, 348, 525, 526
BmiI GGNNCC 2 cut(s) 349, 527
BmrI ACTGGG 2 cut(s) 267, 544
BmsI GCATC 2 cut(s) 14, 505
BmuI ACTGGG 2 cut(s) 267, 544
BoxI GACNNNNGTC 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 492
Bpu14I TTCGAA 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 130
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 644
BsaJI CCNNGG 2 cut(s) 194, 649
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 344, 345, 534, 535
Bse1I ACTGG 2 cut(s) 262, 539
Bse8I GATNNNNATC 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 194, 649
BseGI GGATG 2 cut(s) 5, 496
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 5 cut(s) 195, 344, 345, 534, 535
BseMII CTCAG 3 cut(s) 39, 483, 533
BseNI ACTGG 2 cut(s) 262, 539
BseSI GKGCMC 1 cut(s) 529
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 382
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 203, 527
BsiEI CGRYCG 1 cut(s) 204
BslFI GGGAC 2 cut(s) 361, 631
BslI CCNNNNNNNGG 5 cut(s) 195, 344, 345, 534, 535
BsmFI GGGAC 2 cut(s) 361, 631
BsnI GGCC 2 cut(s) 203, 527
Bsp119I TTCGAA 1 cut(s) 160
Bsp120I GGGCCC 1 cut(s) 525
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 3 cut(s) 544, 636, 706
BspACI CCGC 2 cut(s) 103, 279
BspANI GGCC 2 cut(s) 203, 527
BspCNI CTCAG 3 cut(s) 38, 484, 532
BspLI GGNNCC 2 cut(s) 349, 527
BspMI ACCTGC 1 cut(s) 617
BspQI GCTCTTC 1 cut(s) 434
BspT104I TTCGAA 1 cut(s) 160
BsrI ACTGG 2 cut(s) 262, 539
BssECI CCNNGG 2 cut(s) 194, 649
BssMI GATC 3 cut(s) 544, 636, 706
BssNI GRCGYC 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 649
Bst4CI ACNGT 3 cut(s) 214, 451, 583
Bst6I CTCTTC 4 cut(s) 52, 91, 224, 434
BstACI GRCGYC 1 cut(s) 644
BstBI TTCGAA 1 cut(s) 160
BstDEI CTNAG 4 cut(s) 25, 407, 492, 519
BstF5I GGATG 2 cut(s) 5, 496
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 3 cut(s) 547, 639, 709
BstMBI GATC 3 cut(s) 544, 636, 706
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 26, 524
BstNSI RCATGY 1 cut(s) 478
BstPAI GACNNNNGTC 1 cut(s) 203
BstSFI CTRYAG 1 cut(s) 672
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 636
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 1 cut(s) 636
BstZI CGGCCG 1 cut(s) 201
BsuRI GGCC 2 cut(s) 203, 527
BtsCI GGATG 2 cut(s) 5, 496
BveI ACCTGC 1 cut(s) 617
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 4 cut(s) 276, 348, 525, 526
CseI GACGC 1 cut(s) 633
Csp6I GTAC 1 cut(s) 478
CspCI CAANNNNNGTGG 1 cut(s) 33
CviAII CATG 3 cut(s) 181, 364, 475
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 4 cut(s) 25, 407, 492, 519
DpnI GATC 3 cut(s) 546, 638, 708
DpnII GATC 3 cut(s) 544, 636, 706
DraI TTTAAA 1 cut(s) 718
EaeI YGGCCR 1 cut(s) 201
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 4 cut(s) 52, 91, 224, 434
EarI CTCTTC 4 cut(s) 52, 91, 224, 434
EciI GGCGGA 1 cut(s) 268
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 649
Eco24I GRGCYC 1 cut(s) 529
Eco47I GGWCC 2 cut(s) 276, 348
Eco52I CGGCCG 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 205
EcoO109I RGGNCCY 2 cut(s) 525, 526
EcoRI GAATTC 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 649
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 649
FaeI CATG 3 cut(s) 184, 367, 478
FaiI YATR 6 cut(s) 182, 305, 365, 476, 692, 705
FaqI GGGAC 2 cut(s) 361, 631
FatI CATG 3 cut(s) 180, 363, 474
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 1 cut(s) 396
FokI GGATG 1 cut(s) 483
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 3 cut(s) 75, 392, 650
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 396
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 203, 527
HgaI GACGC 1 cut(s) 633
HhaI GCGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 644
Hin1II CATG 3 cut(s) 184, 367, 478
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 169, 620
HphI GGTGA 1 cut(s) 33
Hpy188I TCNGA 6 cut(s) 168, 198, 472, 522, 549, 613
Hpy188III TCNNGA 2 cut(s) 440, 561
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 3 cut(s) 214, 451, 583
HpyCH4V TGCA 2 cut(s) 20, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 524
HpyF3I CTNAG 4 cut(s) 25, 407, 492, 519
Hsp92I GRCGYC 1 cut(s) 644
Hsp92II CATG 3 cut(s) 184, 367, 478
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 3 cut(s) 544, 636, 706
LguI GCTCTTC 1 cut(s) 434
LpnPI CCDG 9 cut(s) 232, 243, 295, 330, 504, 520, 528, 546, 612
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 14, 505
MaeI CTAG 3 cut(s) 75, 392, 650
MaeIII GTNAC 2 cut(s) 583, 629
MalI GATC 3 cut(s) 546, 638, 708
MboI GATC 3 cut(s) 544, 636, 706
MboII GAAGA 5 cut(s) 39, 78, 101, 211, 421
MflI RGATCY 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 5 cut(s) 162, 311, 420, 454, 686
MlyI GAGTC 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 3 cut(s) 330, 506, 717
MwoI GCNNNNNNNGC 2 cut(s) 26, 524
NdeII GATC 3 cut(s) 544, 636, 706
NlaIII CATG 3 cut(s) 184, 367, 478
NlaIV GGNNCC 2 cut(s) 349, 527
NmeAIII GCCGAG 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 629
NspI RCATGY 1 cut(s) 478
NspV TTCGAA 1 cut(s) 160
PciI ACATGT 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 169
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
PscI ACATGT 1 cut(s) 474
PshAI GACNNNNGTC 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 349, 527
PspOMI GGGCCC 1 cut(s) 525
PspPI GGNCC 4 cut(s) 276, 348, 525, 526
PsuI RGATCY 1 cut(s) 636
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 3 cut(s) 330, 506, 717
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 3 cut(s) 544, 636, 706
Sau96I GGNCC 4 cut(s) 276, 348, 525, 526
SchI GAGTC 1 cut(s) 629
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 2 cut(s) 14, 505
SfcI CTRYAG 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 160
SinI GGWCC 2 cut(s) 276, 348
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 5 cut(s) 162, 311, 420, 454, 686
SsiI CCGC 2 cut(s) 103, 279
SspMI CTAG 3 cut(s) 75, 392, 650
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 3 cut(s) 214, 451, 583
TaqI TCGA 2 cut(s) 160, 439
TaqII GACCGA 1 cut(s) 365
TasI AATT 5 cut(s) 162, 311, 420, 454, 686
TatI WGTACW 1 cut(s) 477
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 3 cut(s) 330, 506, 717
Tru9I TTAA 3 cut(s) 330, 506, 717
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 1 cut(s) 459
VpaK11BI GGWCC 2 cut(s) 276, 348
XapI RAATTY 3 cut(s) 162, 454, 686
XceI RCATGY 1 cut(s) 478
XmaJI CCTAGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 3 cut(s) 75, 392, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.