Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005304

Physical Location & Seq

Genomic Coordinates Reverse (-)

15853921 .. 15863511

Gene Structure 9591 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1078500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGGCATCTTTGTGGTGAAATGGAGAGGGCTCTGGCTTCTTTACGTTCTATTGCTTTATATAACAAGGAATGAAATTAGAGCTATCAGTCCTGATGGTGAGGCACTTCTAAGCTTCAGGACAGCAGTTGTTAGTTCAGATGGTATCCTTCTTCGATGGAGACCAGAAGATCCTGATCCATGTAATTGGAAAGGAGTGAAATGTGATGACAAAACAAAGAGAGTAATCTACTTGAGTCTTGCTGGTCACAAATTAAGTGGATATATACCTCCAGACCTTGGGAAGCTAGATCAGCTGACGATTTTAGCTCTTCATAACAACAACTTTTATGGGACTATTCCTTCAGACCTGGGGAATTGCACACAGTTGGAGAGAATATACTTGCAGGGTAATTACTTAAGTGGGTTAATTCCAAGTGAATTGGGAAAGCTGTCAGCCCTCAAATTTTTAGATATTTCAAGTAACTCTCTCAGTGGAAATATTCCTGTGTCACTAGGGAAGTTGGATAAGCTTGTTGCGCTCAATGTGTCAAACAATTTTCTGGTTGGACCAATACCATGTGATGGAATGCTTACCAACTTACCTGAAAGCTCCTTTGATGGAAATCGTGGTTTGTGTGGGAAACAAATCAATCTGGTATGCAAAGATGTCCCTGGGGAATCACCAAATTCCCAATCTCCAAGCTCAGAGCAAAATCAAGGTGGAAAGAAGAAATACTCTGGTAGGCTACTTATCAGTGCATCAGCAACTGTGGGTGCACTTCTTTTGGTGGCTCTCATGTGTTTCTGGGGTTGCTTTCTTTATAAAAAGTTCGGTAAACATGACAGTAAAGGTCTTGCAATGGATGTTACTGGAGGTGCATCAATTGTAATGTTTCATGGAGATTTGCCATATTCTTCTAAAGACATAATTAAGAAATTAGAGACTTTGAATGATGAACACATAATTGGTGTTGGTGGCTTTGGAACAGTGTACAAGCTTGCAATGGATGACGGCAAAGTATTTGCATTAAAAAGAATTGTAAAGATGAATGAGGGGTTTGATCGTTTTTTCGAGAGGGAGCTAGAAATTCTTGGAAGTATAAAACACCGGTACCTAGTGAATTTGAGGGGCTATTGCAATTCTCCCACATCAAAGTTGCTAATCTATGATTTCTTATCTGGTGGAAGCCTTGATGAAGCACTTCACGAAAGATCTGAGCAGCTTGATTGGGATGCACGATTGAATATTATTTTGGGAGCAGCAAAAGGCCTGGCTTACTTGCATCATGATTGCTCACCTAGGATCATACATCGAGACATAAAGTCCAGTAACATTTTACTTGATGGAAGTCTTGAGGCACGCGTATCAGACTTTGGACTTGCCAAACTGTTGGAGGACGAAGAATCTCACATTACAACAATTGTTGCGGGAACATTTGGTTATCTGGCTCCCGAGTATATGCAAAGTGGCAGAGCAACTGAAAAGACTGATGTTTATAGTTTTGGGGTCCTGGTGCTTGAAGTATTAAGTGGAAAACGACCCACAGATGCATCATTTATTGAGAAGGGCCTCAACATTGTTGGCTGGTTAAATTTTCTGGTCACAGAAAATAGGCAAAGAGAAATTGTTGATCCACAGTGTGAGGGGGTGCAAGCTGAAAGCCTTGACGCCCTGCTCTCTGTCGCCATTCAATGCGTTTCTTCAACTTCAGAAGATCGACCCTCTATGCACAGGGTGGTCCAGCTGCTTGAATCTGAGGTCATGACACCATGCCCTAGTGACTTCTACGATTCCAGCTCTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009653 GO:0009664 GO:0009791 GO:0009826 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0022414 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0044237 GO:0044249 GO:0044464 GO:0045229 GO:0048316 GO:0048354 GO:0048359 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

596

Amino Acids

65.54

Weight (kDa)

5.74

Isoelectric Point (pI)

39.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 69	8e-12	Leucine rich repeat N-terminal domain
LRR_14	PF23598	77 - 177	2.4e-07	Leucine-rich repeat region
LRR_8	PF13855	122 - 179	1.2e-06	Leucine rich repeat
Pkinase	PF00069	312 - 575	6.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	313 - 576	8.2e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005304)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31420 AT1G31420 AT1G31420 AT2G35620 AT2G35620 AT2G35620 AT2G35620
fragaria_vesca	FvH4_5g24350 FvH4_5g24350 FvH4_5g24350 FvH4_5g24350
malus_domestica	MD00G1078500.v1.1 MD06G1021300.v1.1 MD16G1288500.v1.1
prunus_persica	Prupe.5G017300_v2.0.a1
pyrus_communis	pycom06g01550 pycom16g25580
rosa_chinensis	RchiOBHm_Chr7g0214561
rosa_laevigata	RLG00000002732
rosa_multiflora	Rmu_co8061150.1_g000001 Rmu_sc0003490.1_g000001 Rmu_sc0003490.1_g000002
rosa_roxburghii	Rroxscaffold_3G00245210
rosa_rugosa	Rorug07G0146900
rosa_samantha	Rh7AG282000 Rh7BG273800 Rh7CG301300 Rh7DG286000
rosa_wichuraiana	Rw7G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	804
Acc65I	GGTACC	1 cut(s)	1092
AccB1I	GGYRCC	1 cut(s)	1092
AccB7I	CCANNNNNTGG	2 cut(s)	278, 563
AccII	CGCG	1 cut(s)	1344
AciI	CCGC	1 cut(s)	1409
AclWI	GGATC	4 cut(s)	164, 170, 1292, 1607
AcsI	RAATTY	5 cut(s)	443, 667, 1068, 1102, 1573
AcuI	CTGAAG	3 cut(s)	100, 327, 1674
AcyI	GRCGYC	1 cut(s)	1650
AdeI	CACNNNGTG	2 cut(s)	1622, 1717
AfaI	GTAC	2 cut(s)	974, 1094
AfiI	CCNNNNNNNGG	2 cut(s)	278, 563
AflII	CTTAAG	1 cut(s)	397
AflIII	ACRYGT	1 cut(s)	1342
AgeI	ACCGGT	1 cut(s)	1089
AgsI	TTSAA	7 cut(s)	459, 931, 1225, 1502, 1673, 1686, 1733
AhdI	GACNNNNNGTC	1 cut(s)	1303
AjnI	CCWGG	4 cut(s)	348, 652, 1251, 1491
AjuI	GAANNNNNNNTTGG	4 cut(s)	930, 962, 1217, 1249
Alw21I	GWGCWC	1 cut(s)	760
Alw26I	GTCTC	3 cut(s)	154, 917, 1290
Alw44I	GTGCAC	1 cut(s)	756
AlwI	GGATC	4 cut(s)	164, 170, 1292, 1607
AlwNI	CAGNNNCTG	1 cut(s)	749
Ama87I	CYCGRG	1 cut(s)	1433
AoxI	GGCC	2 cut(s)	1249, 1549
ApaLI	GTGCAC	1 cut(s)	756
ApeKI	GCWGC	3 cut(s)	1201, 1241, 1726
ApoI	RAATTY	5 cut(s)	443, 667, 1068, 1102, 1573
AsiGI	ACCGGT	1 cut(s)	1089
Asp700I	GAANNNNTTC	1 cut(s)	1182
Asp718I	GGTACC	1 cut(s)	1092
AspA2I	CCTAGG	1 cut(s)	1280
AspLEI	GCGC	1 cut(s)	520
AspS9I	GGNCC	4 cut(s)	548, 1489, 1549, 1720
AsuHPI	GGTGA	4 cut(s)	28, 110, 654, 1269
AvaI	CYCGRG	1 cut(s)	1433
AvaII	GGWCC	3 cut(s)	548, 1489, 1720
AvrII	CCTAGG	1 cut(s)	1280
BaeGI	GKGCMC	1 cut(s)	760
BaeI	ACNNNNGTAYC	2 cut(s)	1076, 1109
BanI	GGYRCC	1 cut(s)	1092
BanII	GRGCYC	1 cut(s)	33
Bbv12I	GWGCWC	1 cut(s)	760
BbvI	GCAGC	3 cut(s)	1213, 1253, 1713
BccI	CCATC	6 cut(s)	89, 134, 150, 557, 593, 1319
BceAI	ACGGC	1 cut(s)	1009
BciT130I	CCWGG	4 cut(s)	350, 654, 1253, 1493
BciVI	GTATCC	1 cut(s)	155
BcoDI	GTCTC	3 cut(s)	154, 917, 1290
BfaI	CTAG	6 cut(s)	287, 494, 1064, 1097, 1281, 1758
BfrI	CTTAAG	1 cut(s)	397
BfuI	GTATCC	1 cut(s)	155
BglII	AGATCT	1 cut(s)	1193
BisI	GCNGC	3 cut(s)	1202, 1242, 1727
BlnI	CCTAGG	1 cut(s)	1280
BlsI	GCNGC	3 cut(s)	1203, 1243, 1728
Bme1390I	CCNGG	4 cut(s)	350, 654, 1253, 1493
Bme18I	GGWCC	3 cut(s)	548, 1489, 1720
BmeRI	GACNNNNNGTC	1 cut(s)	1303
BmeT110I	CYCGRG	1 cut(s)	1433
BmgT120I	GGNCC	4 cut(s)	548, 1489, 1549, 1720
BmiI	GGNNCC	3 cut(s)	1094, 1431, 1490
BmrFI	CCNGG	4 cut(s)	350, 654, 1253, 1493
BmsI	GCATC	7 cut(s)	15, 749, 869, 1204, 1273, 1519, 1541
BpmI	CTGGAG	2 cut(s)	255, 873
BpuEI	CTTGAG	2 cut(s)	253, 1355
BsaBI	GATNNNNATC	2 cut(s)	174, 603
BsaHI	GRCGYC	1 cut(s)	1650
BsaI	GGTCTC	1 cut(s)	154
BsaJI	CCNNGG	5 cut(s)	277, 349, 652, 653, 1280
BsaWI	WCCGGW	1 cut(s)	1089
Bsc4I	CCNNNNNNNGG	2 cut(s)	278, 563
Bse118I	RCCGGY	1 cut(s)	1089
Bse1I	ACTGG	2 cut(s)	856, 1308
Bse3DI	GCAATG	2 cut(s)	846, 990
Bse8I	GATNNNNATC	2 cut(s)	174, 603
BseBI	CCWGG	4 cut(s)	350, 654, 1253, 1493
BseDI	CCNNGG	5 cut(s)	277, 349, 652, 653, 1280
BseGI	GGATG	3 cut(s)	850, 994, 1219
BseJI	GATNNNNATC	2 cut(s)	174, 603
BseLI	CCNNNNNNNGG	2 cut(s)	278, 563
BseMI	GCAATG	2 cut(s)	846, 990
BseMII	CTCAG	5 cut(s)	484, 699, 1188, 1728, 1773
BseNI	ACTGG	2 cut(s)	856, 1308
BseSI	GKGCMC	1 cut(s)	760
BseXI	GCAGC	3 cut(s)	1213, 1253, 1713
Bsh1236I	CGCG	1 cut(s)	1344
BshFI	GGCC	2 cut(s)	1251, 1551
BshNI	GGYRCC	1 cut(s)	1092
BshTI	ACCGGT	1 cut(s)	1089
BsiHKAI	GWGCWC	1 cut(s)	760
BsiHKCI	CYCGRG	1 cut(s)	1433
BsiSI	CCGG	1 cut(s)	1090
BslFI	GGGAC	2 cut(s)	346, 635
BslI	CCNNNNNNNGG	2 cut(s)	278, 563
BsmAI	GTCTC	3 cut(s)	154, 917, 1290
BsmFI	GGGAC	2 cut(s)	346, 635
BsmI	GAATGC	1 cut(s)	573
BsnI	GGCC	2 cut(s)	1251, 1551
Bso31I	GGTCTC	1 cut(s)	154
BsoBI	CYCGRG	1 cut(s)	1433
Bsp1286I	GDGCHC	2 cut(s)	33, 760
Bsp1407I	TGTACA	1 cut(s)	972
Bsp143I	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
BspACI	CCGC	1 cut(s)	1409
BspANI	GGCC	2 cut(s)	1251, 1551
BspCNI	CTCAG	5 cut(s)	483, 698, 1189, 1729, 1774
BspFNI	CGCG	1 cut(s)	1344
BspHI	TCATGA	2 cut(s)	1267, 1743
BspLI	GGNNCC	3 cut(s)	1094, 1431, 1490
BspPI	GGATC	4 cut(s)	164, 170, 1292, 1607
BspQI	GCTCTTC	1 cut(s)	315
BspT107I	GGYRCC	1 cut(s)	1092
BspTI	CTTAAG	1 cut(s)	397
BspTNI	GGTCTC	1 cut(s)	154
BsrDI	GCAATG	2 cut(s)	846, 990
BsrFI	RCCGGY	1 cut(s)	1089
BsrGI	TGTACA	1 cut(s)	972
BsrI	ACTGG	2 cut(s)	856, 1308
BssAI	RCCGGY	1 cut(s)	1089
BssECI	CCNNGG	5 cut(s)	277, 349, 652, 653, 1280
BssMI	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
BssNI	GRCGYC	1 cut(s)	1650
BssT1I	CCWWGG	2 cut(s)	277, 1280
Bst2UI	CCWGG	4 cut(s)	350, 654, 1253, 1493
Bst4CI	ACNGT	6 cut(s)	366, 751, 827, 970, 1371, 1620
Bst6I	CTCTTC	1 cut(s)	315
BstACI	GRCGYC	1 cut(s)	1650
BstAFI	CTTAAG	1 cut(s)	397
BstAUI	TGTACA	1 cut(s)	972
BstC8I	GCNNGC	3 cut(s)	981, 1342, 1635
BstDEI	CTNAG	6 cut(s)	110, 470, 685, 1197, 1737, 1782
BstF5I	GGATG	3 cut(s)	850, 994, 1219
BstFNI	CGCG	1 cut(s)	1344
BstHHI	GCGC	1 cut(s)	520
BstKTI	GATC	8 cut(s)	172, 178, 292, 1045, 1196, 1287, 1615, 1699
BstMAI	GTCTC	3 cut(s)	154, 917, 1290
BstMBI	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
BstMWI	GCNNNNNNNGC	2 cut(s)	292, 517
BstNI	CCWGG	4 cut(s)	350, 654, 1253, 1493
BstSCI	CCNGG	4 cut(s)	348, 652, 1251, 1491
BstSLI	GKGCMC	1 cut(s)	760
BstUI	CGCG	1 cut(s)	1344
BstV1I	GCAGC	3 cut(s)	1213, 1253, 1713
BstX2I	RGATCY	2 cut(s)	169, 1193
BstXI	CCANNNNNNTGG	2 cut(s)	186, 1372
BstYI	RGATCY	2 cut(s)	169, 1193
BsuI	GTATCC	1 cut(s)	155
BsuRI	GGCC	2 cut(s)	1251, 1551
BtsCI	GGATG	3 cut(s)	850, 994, 1219
BtsIMutI	CAGTG	4 cut(s)	478, 742, 975, 1625
Cac8I	GCNNGC	3 cut(s)	981, 1342, 1635
CaiI	CAGNNNCTG	1 cut(s)	749
CciI	TCATGA	2 cut(s)	1267, 1743
CfoI	GCGC	1 cut(s)	520
Cfr10I	RCCGGY	1 cut(s)	1089
Cfr13I	GGNCC	4 cut(s)	548, 1489, 1549, 1720
CseI	GACGC	1 cut(s)	1658
Csp6I	GTAC	2 cut(s)	973, 1093
CspAI	ACCGGT	1 cut(s)	1089
CspCI	CAANNNNNGTGG	2 cut(s)	238, 273
CviAII	CATG	8 cut(s)	180, 558, 778, 821, 878, 1268, 1744, 1752
CviQI	GTAC	2 cut(s)	973, 1093
DdeI	CTNAG	6 cut(s)	110, 470, 685, 1197, 1737, 1782
DpnI	GATC	8 cut(s)	171, 177, 291, 1044, 1195, 1286, 1614, 1698
DpnII	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
DraIII	CACNNNGTG	2 cut(s)	1622, 1717
DriI	GACNNNNNGTC	1 cut(s)	1303
Eam1104I	CTCTTC	1 cut(s)	315
Eam1105I	GACNNNNNGTC	1 cut(s)	1303
EarI	CTCTTC	1 cut(s)	315
Eco130I	CCWWGG	2 cut(s)	277, 1280
Eco147I	AGGCCT	1 cut(s)	1251
Eco24I	GRGCYC	1 cut(s)	33
Eco31I	GGTCTC	1 cut(s)	154
Eco47I	GGWCC	3 cut(s)	548, 1489, 1720
Eco57I	CTGAAG	3 cut(s)	100, 327, 1674
Eco88I	CYCGRG	1 cut(s)	1433
EcoO109I	RGGNCCY	2 cut(s)	1489, 1549
EcoRII	CCWGG	4 cut(s)	348, 652, 1251, 1491
EcoT14I	CCWWGG	2 cut(s)	277, 1280
EcoT22I	ATGCAT	1 cut(s)	1534
EcoT38I	GRGCYC	1 cut(s)	33
ErhI	CCWWGG	2 cut(s)	277, 1280
FaeI	CATG	8 cut(s)	183, 561, 781, 824, 881, 1271, 1747, 1755
FaqI	GGGAC	2 cut(s)	346, 635
FatI	CATG	8 cut(s)	179, 557, 777, 820, 877, 1267, 1743, 1751
FauI	CCCGC	1 cut(s)	1402
Fnu4HI	GCNGC	3 cut(s)	1202, 1242, 1727
FokI	GGATG	3 cut(s)	857, 1001, 1226
FriOI	GRGCYC	1 cut(s)	33
Fsp4HI	GCNGC	3 cut(s)	1202, 1242, 1727
FspBI	CTAG	6 cut(s)	287, 494, 1064, 1097, 1281, 1758
GlaI	GCGC	1 cut(s)	519
GluI	GCNGC	3 cut(s)	1202, 1242, 1727
GsuI	CTGGAG	2 cut(s)	255, 873
HaeIII	GGCC	2 cut(s)	1251, 1551
HapII	CCGG	1 cut(s)	1090
HgaI	GACGC	1 cut(s)	1658
HhaI	GCGC	1 cut(s)	520
Hin1I	GRCGYC	1 cut(s)	1650
Hin1II	CATG	8 cut(s)	183, 561, 781, 824, 881, 1271, 1747, 1755
Hin6I	GCGC	1 cut(s)	518
HinP1I	GCGC	1 cut(s)	518
HindIII	AAGCTT	3 cut(s)	112, 509, 977
HinfI	GANTC	5 cut(s)	235, 659, 1385, 1733, 1772
HpaII	CCGG	1 cut(s)	1090
HphI	GGTGA	4 cut(s)	28, 110, 654, 1269
Hpy166II	GTNNAC	3 cut(s)	758, 818, 973
Hpy188I	TCNGA	8 cut(s)	139, 346, 688, 1198, 1351, 1693, 1738, 1783
Hpy8I	GTNNAC	3 cut(s)	758, 818, 973
HpyAV	CCTTC	3 cut(s)	158, 351, 1540
HpyCH4III	ACNGT	6 cut(s)	366, 751, 827, 970, 1371, 1620
HpyCH4IV	ACGT	1 cut(s)	45
HpyF10VI	GCNNNNNNNGC	2 cut(s)	292, 517
HpyF3I	CTNAG	6 cut(s)	110, 470, 685, 1197, 1737, 1782
HpySE526I	ACGT	1 cut(s)	45
Hsp92I	GRCGYC	1 cut(s)	1650
Hsp92II	CATG	8 cut(s)	183, 561, 781, 824, 881, 1271, 1747, 1755
HspAI	GCGC	1 cut(s)	518
KpnI	GGTACC	1 cut(s)	1096
Kzo9I	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
LguI	GCTCTTC	1 cut(s)	315
LmnI	GCTCC	4 cut(s)	596, 1060, 1238, 1435
Lsp1109I	GCAGC	3 cut(s)	1213, 1253, 1713
LweI	GCATC	7 cut(s)	15, 749, 869, 1204, 1273, 1519, 1541
MaeI	CTAG	6 cut(s)	287, 494, 1064, 1097, 1281, 1758
MaeII	ACGT	1 cut(s)	45
MaeIII	GTNAC	7 cut(s)	245, 461, 489, 847, 1310, 1582, 1760
MalI	GATC	8 cut(s)	171, 177, 291, 1044, 1195, 1286, 1614, 1698
MboI	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
MboII	GAAGA	8 cut(s)	143, 179, 302, 721, 888, 1394, 1674, 1706
MfeI	CAATTG	2 cut(s)	864, 1401
MflI	RGATCY	2 cut(s)	169, 1193
MhlI	GDGCHC	2 cut(s)	33, 760
MluI	ACGCGT	1 cut(s)	1342
MlyI	GAGTC	1 cut(s)	244
MmeI	TCCRAC	4 cut(s)	348, 483, 526, 1353
Mph1103I	ATGCAT	1 cut(s)	1534
MroXI	GAANNNNTTC	1 cut(s)	1182
MseI	TTAA	7 cut(s)	254, 398, 407, 912, 1010, 1508, 1571
MslI	CAYNNNNRTG	1 cut(s)	11
MspA1I	CMGCKG	2 cut(s)	295, 1726
MspCI	CTTAAG	1 cut(s)	397
MspI	CCGG	1 cut(s)	1090
MspR9I	CCNGG	4 cut(s)	350, 654, 1253, 1493
MunI	CAATTG	2 cut(s)	864, 1401
Mva1269I	GAATGC	1 cut(s)	573
MvaI	CCWGG	4 cut(s)	350, 654, 1253, 1493
MvnI	CGCG	1 cut(s)	1344
MwoI	GCNNNNNNNGC	2 cut(s)	292, 517
NdeII	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
NlaIII	CATG	8 cut(s)	183, 561, 781, 824, 881, 1271, 1747, 1755
NlaIV	GGNNCC	3 cut(s)	1094, 1431, 1490
NmuCI	GTSAC	4 cut(s)	245, 489, 1582, 1760
NsiI	ATGCAT	1 cut(s)	1534
PagI	TCATGA	2 cut(s)	1267, 1743
PasI	CCCWGGG	1 cut(s)	653
PceI	AGGCCT	1 cut(s)	1251
PciSI	GCTCTTC	1 cut(s)	315
PctI	GAATGC	1 cut(s)	573
PdmI	GAANNNNTTC	1 cut(s)	1182
PfeI	GAWTC	4 cut(s)	659, 1385, 1733, 1772
PflMI	CCANNNNNTGG	2 cut(s)	278, 563
PinAI	ACCGGT	1 cut(s)	1089
PkrI	GCNGC	3 cut(s)	1203, 1243, 1728
PleI	GAGTC	1 cut(s)	243
PpsI	GAGTC	1 cut(s)	243
PpuMI	RGGWCCY	1 cut(s)	1489
PsiI	TTATAA	1 cut(s)	804
Psp5II	RGGWCCY	1 cut(s)	1489
Psp6I	CCWGG	4 cut(s)	348, 652, 1251, 1491
PspGI	CCWGG	4 cut(s)	348, 652, 1251, 1491
PspN4I	GGNNCC	3 cut(s)	1094, 1431, 1490
PspPI	GGNCC	4 cut(s)	548, 1489, 1549, 1720
PspPPI	RGGWCCY	1 cut(s)	1489
PstNI	CAGNNNCTG	1 cut(s)	749
PsuI	RGATCY	2 cut(s)	169, 1193
PvuII	CAGCTG	2 cut(s)	295, 1726
RsaI	GTAC	2 cut(s)	974, 1094
RsaNI	GTAC	2 cut(s)	973, 1093
RseI	CAYNNNNRTG	1 cut(s)	11
SapI	GCTCTTC	1 cut(s)	315
SaqAI	TTAA	7 cut(s)	254, 398, 407, 912, 1010, 1508, 1571
SatI	GCNGC	3 cut(s)	1202, 1242, 1727
Sau3AI	GATC	8 cut(s)	169, 175, 289, 1042, 1193, 1284, 1612, 1696
Sau96I	GGNCC	4 cut(s)	548, 1489, 1549, 1720
SchI	GAGTC	1 cut(s)	244
ScrFI	CCNGG	4 cut(s)	350, 654, 1253, 1493
SduI	GDGCHC	2 cut(s)	33, 760
SfaNI	GCATC	7 cut(s)	15, 749, 869, 1204, 1273, 1519, 1541
SinI	GGWCC	3 cut(s)	548, 1489, 1720
SmiMI	CAYNNNNRTG	1 cut(s)	11
SmlI	CTYRAG	3 cut(s)	232, 397, 1334
SmoI	CTYRAG	3 cut(s)	232, 397, 1334
SseBI	AGGCCT	1 cut(s)	1251
SsiI	CCGC	1 cut(s)	1409
SspI	AATATT	2 cut(s)	481, 1228
SspMI	CTAG	6 cut(s)	287, 494, 1064, 1097, 1281, 1758
StuI	AGGCCT	1 cut(s)	1251
StyD4I	CCNGG	4 cut(s)	348, 652, 1251, 1491
StyI	CCWWGG	2 cut(s)	277, 1280
TaaI	ACNGT	6 cut(s)	366, 751, 827, 970, 1371, 1620
TaiI	ACGT	1 cut(s)	48
TaqI	TCGA	4 cut(s)	154, 1053, 1294, 1699
TatI	WGTACW	1 cut(s)	972
TfiI	GAWTC	4 cut(s)	659, 1385, 1733, 1772
Tru1I	TTAA	7 cut(s)	254, 398, 407, 912, 1010, 1508, 1571
Tru9I	TTAA	7 cut(s)	254, 398, 407, 912, 1010, 1508, 1571
TscAI	CASTG	4 cut(s)	478, 742, 975, 1625
TseFI	GTSAC	4 cut(s)	245, 489, 1582, 1760
TseI	GCWGC	3 cut(s)	1201, 1241, 1726
Tsp45I	GTSAC	4 cut(s)	245, 489, 1582, 1760
TspDTI	ATGAA	6 cut(s)	87, 302, 866, 951, 1043, 1191
TspRI	CASTG	4 cut(s)	478, 742, 975, 1625
Van91I	CCANNNNNTGG	2 cut(s)	278, 563
Vha464I	CTTAAG	1 cut(s)	397
VneI	GTGCAC	1 cut(s)	756
VpaK11BI	GGWCC	3 cut(s)	548, 1489, 1720
XapI	RAATTY	5 cut(s)	443, 667, 1068, 1102, 1573
XmaJI	CCTAGG	1 cut(s)	1280
XmnI	GAANNNNTTC	1 cut(s)	1182
XspI	CTAG	6 cut(s)	287, 494, 1064, 1097, 1281, 1758
Zsp2I	ATGCAT	1 cut(s)	1534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.