Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005304

Physical Location & Seq

Genomic Coordinates Reverse (-)

41377292 .. 41384270

Gene Structure 6979 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1288500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGTGTAGAATATTAACTTATTTTGCCCGGGTAATTGTGCGCTTTCTGTCGGGTCACAAATTAAGTGGATATATACCTCCAGACCTTGGGAAGCTAGATCAGCTGACGATTTTAGCTCTTCATAACAACAACTTTTATGGGACTATTCCTTCAGACCTGGGGAATTGCACACAGTTGGAGAGAATATACTTGCAGGGTAATTACTTAAGTGGGTTAATTCCAAGTGAATTGGGAAAGCTGTCAGCCCTCAAATTTTTAGATATTTCAAGTAACTCTCTCAGTGGAAATATTCCTGTGTCACTAGGGAAGTTGGATAAGCTTGTTGCGCTCAATGTGTCAAACAATTTTCTGGTTGGACCAATACCATGTGATGGAATGCTTACCAACTTACCTGAAAGCTCCTTTGATGGAAATCGTGGTTTGTGTGGGAAACAAATCAATCTGGTATGCAAAGATGTCCCTGGGGAATCACCAAATTCCCAATCTCCAAGCTCAGAGCAAAATCAAGGTGGAAAGAAGAAATACTCTGGTAGGCTACTTATCAGTGCATCAGCAACTGTGGGTGCACTTCTTTTGGTGGCTCTCATGTGTTTCTGGGGTTGCTTTCTTTATAAAAAGTTCGGTAAACATGACAGTAAAGGTCTTGCAATGGATGTTACTGGAGGTGCATCAATTGTAATGTTTCATGGAGATTTGCCATATTCTTCTAAAGACATAATTAAGAAATTAGAGACTTTGAATGATGAACACATAATTGGTGTTGGTGGCTTTGGAACAGTGTACAAGCTTGCAATGGATGACGGCAAAGTATTTGCATTAAAAAGAATTGTAAAGATGAATGAGGGGTTTGATCGTTTTTTCGAGAGGGAGCTAGAAATTCTTGGAAGTATAAAACACCGGTACCTAGTGAATTTGAGGGGCTATTGCAATTCTCCCACATCAAAGTTGCTAATCTATGATTTCTTATCTGGTGGAAGCCTTGATGAAGCACTTCACGAAAGATCTGAGCAGCTTGATTGGGATGCACGATTGAATATTATTTTGGGAGCAGCAAAAGGCCTGGCTTACTTGCATCATGATTGCTCACCTAGGATCATACATCGAGACATAAAGTCCAGTAACATTTTACTTGATGGAAGTCTTGAGGCACGCGTATCAGACTTTGGACTTGCCAAACTGTTGGAGGACGAAGAATCTCACATTACAACAATTGTTGCGGGAACATTTGGTTATCTGGCTCCCGAGTATATGCAAAGTGGCAGAGCAACTGAAAAGACTGATGTTTATAGTTTTGGGGTCCTGGTGCTTGAAGTATTAAGTGGAAAACGACCCACAGATGCATCATTTATTGAGAAGGGCCTCAACATTGTTGGCTGGTTAAATTTTCTGGTCACAGAAAATAGGCAAAGAGAAATTGTTGATCCACAGTGTGAGGGGGTGCAAGCTGAAAGCCTTGACGCCCTGCTCTCTGTCGCCATTCAATGCGTTTCTTCAACTTCAGAAGATCGACCCTCTATGCACAGGGTGGTCCAGCTGCTTGAATCTGAGGTCATGACACCATGCCCTAGTGACTTCTACGATTCCAGCTCTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009653 GO:0009664 GO:0009791 GO:0009826 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0022414 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0044237 GO:0044249 GO:0044464 GO:0045229 GO:0048316 GO:0048354 GO:0048359 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

532

Amino Acids

58.27

Weight (kDa)

5.72

Isoelectric Point (pI)

38.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	27 - 114	8.4e-07	Leucine-rich repeat region
LRR_8	PF13855	34 - 93	8.2e-07	Leucine rich repeat
LRR_8	PF13855	58 - 115	8.7e-07	Leucine rich repeat
Pkinase	PF00069	248 - 511	5.1e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	249 - 512	6.2e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005304)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31420 AT1G31420 AT1G31420 AT2G35620 AT2G35620 AT2G35620 AT2G35620
fragaria_vesca	FvH4_5g24350 FvH4_5g24350 FvH4_5g24350 FvH4_5g24350
malus_domestica	MD00G1078500.v1.1 MD06G1021300.v1.1 MD16G1288500.v1.1
prunus_persica	Prupe.5G017300_v2.0.a1
pyrus_communis	pycom06g01550 pycom16g25580
rosa_chinensis	RchiOBHm_Chr7g0214561
rosa_laevigata	RLG00000002732
rosa_multiflora	Rmu_co8061150.1_g000001 Rmu_sc0003490.1_g000001 Rmu_sc0003490.1_g000002
rosa_roxburghii	Rroxscaffold_3G00245210
rosa_rugosa	Rorug07G0146900
rosa_samantha	Rh7AG282000 Rh7BG273800 Rh7CG301300 Rh7DG286000
rosa_wichuraiana	Rw7G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	612
Acc65I	GGTACC	1 cut(s)	900
AccB1I	GGYRCC	1 cut(s)	900
AccB7I	CCANNNNNTGG	2 cut(s)	86, 371
AccII	CGCG	1 cut(s)	1152
AciI	CCGC	1 cut(s)	1217
AclWI	GGATC	2 cut(s)	1100, 1415
AcsI	RAATTY	5 cut(s)	251, 475, 876, 910, 1381
AcuI	CTGAAG	2 cut(s)	135, 1482
AcyI	GRCGYC	1 cut(s)	1458
AdeI	CACNNNGTG	2 cut(s)	1430, 1525
AfaI	GTAC	2 cut(s)	782, 902
AfiI	CCNNNNNNNGG	2 cut(s)	86, 371
AflII	CTTAAG	1 cut(s)	205
AflIII	ACRYGT	1 cut(s)	1150
AgeI	ACCGGT	1 cut(s)	897
AgsI	TTSAA	7 cut(s)	267, 739, 1033, 1310, 1481, 1494, 1541
AhdI	GACNNNNNGTC	1 cut(s)	1111
AjnI	CCWGG	4 cut(s)	156, 460, 1059, 1299
AjuI	GAANNNNNNNTTGG	4 cut(s)	738, 770, 1025, 1057
Alw21I	GWGCWC	1 cut(s)	568
Alw26I	GTCTC	2 cut(s)	725, 1098
Alw44I	GTGCAC	1 cut(s)	564
AlwI	GGATC	2 cut(s)	1100, 1415
AlwNI	CAGNNNCTG	1 cut(s)	557
Ama87I	CYCGRG	2 cut(s)	27, 1241
AoxI	GGCC	2 cut(s)	1057, 1357
ApaLI	GTGCAC	1 cut(s)	564
ApeKI	GCWGC	3 cut(s)	1009, 1049, 1534
ApoI	RAATTY	5 cut(s)	251, 475, 876, 910, 1381
AsiGI	ACCGGT	1 cut(s)	897
Asp700I	GAANNNNTTC	1 cut(s)	990
Asp718I	GGTACC	1 cut(s)	900
AspA2I	CCTAGG	1 cut(s)	1088
AspLEI	GCGC	2 cut(s)	42, 328
AspS9I	GGNCC	4 cut(s)	356, 1297, 1357, 1528
AsuC2I	CCSGG	2 cut(s)	28, 29
AsuHPI	GGTGA	2 cut(s)	462, 1077
AvaI	CYCGRG	2 cut(s)	27, 1241
AvaII	GGWCC	3 cut(s)	356, 1297, 1528
AvrII	CCTAGG	1 cut(s)	1088
BaeGI	GKGCMC	1 cut(s)	568
BaeI	ACNNNNGTAYC	2 cut(s)	884, 917
BanI	GGYRCC	1 cut(s)	900
Bbv12I	GWGCWC	1 cut(s)	568
BbvI	GCAGC	3 cut(s)	1021, 1061, 1521
BccI	CCATC	3 cut(s)	365, 401, 1127
BceAI	ACGGC	1 cut(s)	817
BciT130I	CCWGG	4 cut(s)	158, 462, 1061, 1301
BcnI	CCSGG	2 cut(s)	28, 29
BcoDI	GTCTC	2 cut(s)	725, 1098
BfaI	CTAG	6 cut(s)	95, 302, 872, 905, 1089, 1566
BfrI	CTTAAG	1 cut(s)	205
BglII	AGATCT	1 cut(s)	1001
BisI	GCNGC	3 cut(s)	1010, 1050, 1535
BlnI	CCTAGG	1 cut(s)	1088
BlsI	GCNGC	3 cut(s)	1011, 1051, 1536
Bme1390I	CCNGG	6 cut(s)	28, 29, 158, 462, 1061, 1301
Bme18I	GGWCC	3 cut(s)	356, 1297, 1528
BmeRI	GACNNNNNGTC	1 cut(s)	1111
BmeT110I	CYCGRG	2 cut(s)	27, 1241
BmgT120I	GGNCC	4 cut(s)	356, 1297, 1357, 1528
BmiI	GGNNCC	3 cut(s)	902, 1239, 1298
BmrFI	CCNGG	6 cut(s)	28, 29, 158, 462, 1061, 1301
BmsI	GCATC	6 cut(s)	557, 677, 1012, 1081, 1327, 1349
BpmI	CTGGAG	2 cut(s)	63, 681
BpuEI	CTTGAG	1 cut(s)	1163
BpuMI	CCSGG	2 cut(s)	28, 29
BsaBI	GATNNNNATC	1 cut(s)	411
BsaHI	GRCGYC	1 cut(s)	1458
BsaJI	CCNNGG	6 cut(s)	27, 85, 157, 460, 461, 1088
BsaWI	WCCGGW	1 cut(s)	897
Bsc4I	CCNNNNNNNGG	2 cut(s)	86, 371
Bse118I	RCCGGY	1 cut(s)	897
Bse1I	ACTGG	2 cut(s)	664, 1116
Bse3DI	GCAATG	2 cut(s)	654, 798
Bse8I	GATNNNNATC	1 cut(s)	411
BseBI	CCWGG	4 cut(s)	158, 462, 1061, 1301
BseDI	CCNNGG	6 cut(s)	27, 85, 157, 460, 461, 1088
BseGI	GGATG	3 cut(s)	658, 802, 1027
BseJI	GATNNNNATC	1 cut(s)	411
BseLI	CCNNNNNNNGG	2 cut(s)	86, 371
BseMI	GCAATG	2 cut(s)	654, 798
BseMII	CTCAG	5 cut(s)	292, 507, 996, 1536, 1581
BseNI	ACTGG	2 cut(s)	664, 1116
BseSI	GKGCMC	1 cut(s)	568
BseXI	GCAGC	3 cut(s)	1021, 1061, 1521
Bsh1236I	CGCG	1 cut(s)	1152
BshFI	GGCC	2 cut(s)	1059, 1359
BshNI	GGYRCC	1 cut(s)	900
BshTI	ACCGGT	1 cut(s)	897
BsiHKAI	GWGCWC	1 cut(s)	568
BsiHKCI	CYCGRG	2 cut(s)	27, 1241
BsiSI	CCGG	2 cut(s)	28, 898
BslFI	GGGAC	2 cut(s)	154, 443
BslI	CCNNNNNNNGG	2 cut(s)	86, 371
BsmAI	GTCTC	2 cut(s)	725, 1098
BsmFI	GGGAC	2 cut(s)	154, 443
BsmI	GAATGC	1 cut(s)	381
BsnI	GGCC	2 cut(s)	1059, 1359
BsoBI	CYCGRG	2 cut(s)	27, 1241
Bsp1286I	GDGCHC	1 cut(s)	568
Bsp1407I	TGTACA	1 cut(s)	780
Bsp143I	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
BspACI	CCGC	1 cut(s)	1217
BspANI	GGCC	2 cut(s)	1059, 1359
BspCNI	CTCAG	5 cut(s)	291, 506, 997, 1537, 1582
BspFNI	CGCG	1 cut(s)	1152
BspHI	TCATGA	2 cut(s)	1075, 1551
BspLI	GGNNCC	3 cut(s)	902, 1239, 1298
BspPI	GGATC	2 cut(s)	1100, 1415
BspQI	GCTCTTC	1 cut(s)	123
BspT107I	GGYRCC	1 cut(s)	900
BspTI	CTTAAG	1 cut(s)	205
BsrDI	GCAATG	2 cut(s)	654, 798
BsrFI	RCCGGY	1 cut(s)	897
BsrGI	TGTACA	1 cut(s)	780
BsrI	ACTGG	2 cut(s)	664, 1116
BssAI	RCCGGY	1 cut(s)	897
BssECI	CCNNGG	6 cut(s)	27, 85, 157, 460, 461, 1088
BssMI	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
BssNI	GRCGYC	1 cut(s)	1458
BssT1I	CCWWGG	2 cut(s)	85, 1088
Bst2UI	CCWGG	4 cut(s)	158, 462, 1061, 1301
Bst4CI	ACNGT	6 cut(s)	174, 559, 635, 778, 1179, 1428
Bst6I	CTCTTC	1 cut(s)	123
BstACI	GRCGYC	1 cut(s)	1458
BstAFI	CTTAAG	1 cut(s)	205
BstAUI	TGTACA	1 cut(s)	780
BstC8I	GCNNGC	3 cut(s)	789, 1150, 1443
BstDEI	CTNAG	5 cut(s)	278, 493, 1005, 1545, 1590
BstF5I	GGATG	3 cut(s)	658, 802, 1027
BstFNI	CGCG	1 cut(s)	1152
BstHHI	GCGC	2 cut(s)	42, 328
BstKTI	GATC	6 cut(s)	100, 853, 1004, 1095, 1423, 1507
BstMAI	GTCTC	2 cut(s)	725, 1098
BstMBI	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
BstMWI	GCNNNNNNNGC	2 cut(s)	100, 325
BstNI	CCWGG	4 cut(s)	158, 462, 1061, 1301
BstSCI	CCNGG	6 cut(s)	26, 27, 156, 460, 1059, 1299
BstSLI	GKGCMC	1 cut(s)	568
BstUI	CGCG	1 cut(s)	1152
BstV1I	GCAGC	3 cut(s)	1021, 1061, 1521
BstX2I	RGATCY	1 cut(s)	1001
BstXI	CCANNNNNNTGG	1 cut(s)	1180
BstYI	RGATCY	1 cut(s)	1001
BsuRI	GGCC	2 cut(s)	1059, 1359
BtsCI	GGATG	3 cut(s)	658, 802, 1027
BtsIMutI	CAGTG	4 cut(s)	286, 550, 783, 1433
Cac8I	GCNNGC	3 cut(s)	789, 1150, 1443
CaiI	CAGNNNCTG	1 cut(s)	557
CciI	TCATGA	2 cut(s)	1075, 1551
CfoI	GCGC	2 cut(s)	42, 328
Cfr10I	RCCGGY	1 cut(s)	897
Cfr13I	GGNCC	4 cut(s)	356, 1297, 1357, 1528
Cfr9I	CCCGGG	1 cut(s)	27
CseI	GACGC	1 cut(s)	1466
Csp6I	GTAC	2 cut(s)	781, 901
CspAI	ACCGGT	1 cut(s)	897
CspCI	CAANNNNNGTGG	2 cut(s)	46, 81
CviAII	CATG	7 cut(s)	366, 586, 629, 686, 1076, 1552, 1560
CviQI	GTAC	2 cut(s)	781, 901
DdeI	CTNAG	5 cut(s)	278, 493, 1005, 1545, 1590
DpnI	GATC	6 cut(s)	99, 852, 1003, 1094, 1422, 1506
DpnII	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
DraIII	CACNNNGTG	2 cut(s)	1430, 1525
DriI	GACNNNNNGTC	1 cut(s)	1111
Eam1104I	CTCTTC	1 cut(s)	123
Eam1105I	GACNNNNNGTC	1 cut(s)	1111
EarI	CTCTTC	1 cut(s)	123
Eco130I	CCWWGG	2 cut(s)	85, 1088
Eco147I	AGGCCT	1 cut(s)	1059
Eco47I	GGWCC	3 cut(s)	356, 1297, 1528
Eco57I	CTGAAG	2 cut(s)	135, 1482
Eco88I	CYCGRG	2 cut(s)	27, 1241
EcoO109I	RGGNCCY	2 cut(s)	1297, 1357
EcoRII	CCWGG	4 cut(s)	156, 460, 1059, 1299
EcoT14I	CCWWGG	2 cut(s)	85, 1088
EcoT22I	ATGCAT	1 cut(s)	1342
ErhI	CCWWGG	2 cut(s)	85, 1088
FaeI	CATG	7 cut(s)	369, 589, 632, 689, 1079, 1555, 1563
FaqI	GGGAC	2 cut(s)	154, 443
FatI	CATG	7 cut(s)	365, 585, 628, 685, 1075, 1551, 1559
FauI	CCCGC	1 cut(s)	1210
Fnu4HI	GCNGC	3 cut(s)	1010, 1050, 1535
FokI	GGATG	3 cut(s)	665, 809, 1034
Fsp4HI	GCNGC	3 cut(s)	1010, 1050, 1535
FspBI	CTAG	6 cut(s)	95, 302, 872, 905, 1089, 1566
GlaI	GCGC	2 cut(s)	41, 327
GluI	GCNGC	3 cut(s)	1010, 1050, 1535
GsuI	CTGGAG	2 cut(s)	63, 681
HaeIII	GGCC	2 cut(s)	1059, 1359
HapII	CCGG	2 cut(s)	28, 898
HgaI	GACGC	1 cut(s)	1466
HhaI	GCGC	2 cut(s)	42, 328
Hin1I	GRCGYC	1 cut(s)	1458
Hin1II	CATG	7 cut(s)	369, 589, 632, 689, 1079, 1555, 1563
Hin6I	GCGC	2 cut(s)	40, 326
HinP1I	GCGC	2 cut(s)	40, 326
HindIII	AAGCTT	2 cut(s)	317, 785
HinfI	GANTC	4 cut(s)	467, 1193, 1541, 1580
HpaII	CCGG	2 cut(s)	28, 898
HphI	GGTGA	2 cut(s)	462, 1077
Hpy166II	GTNNAC	3 cut(s)	566, 626, 781
Hpy188I	TCNGA	7 cut(s)	154, 496, 1006, 1159, 1501, 1546, 1591
Hpy188III	TCNNGA	8 cut(s)	80, 862, 995, 1076, 1103, 1142, 1241, 1552
Hpy8I	GTNNAC	3 cut(s)	566, 626, 781
HpyAV	CCTTC	2 cut(s)	159, 1348
HpyCH4III	ACNGT	6 cut(s)	174, 559, 635, 778, 1179, 1428
HpyF10VI	GCNNNNNNNGC	2 cut(s)	100, 325
HpyF3I	CTNAG	5 cut(s)	278, 493, 1005, 1545, 1590
Hsp92I	GRCGYC	1 cut(s)	1458
Hsp92II	CATG	7 cut(s)	369, 589, 632, 689, 1079, 1555, 1563
HspAI	GCGC	2 cut(s)	40, 326
KpnI	GGTACC	1 cut(s)	904
Kzo9I	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
LguI	GCTCTTC	1 cut(s)	123
LmnI	GCTCC	4 cut(s)	404, 868, 1046, 1243
Lsp1109I	GCAGC	3 cut(s)	1021, 1061, 1521
LweI	GCATC	6 cut(s)	557, 677, 1012, 1081, 1327, 1349
MaeI	CTAG	6 cut(s)	95, 302, 872, 905, 1089, 1566
MaeIII	GTNAC	7 cut(s)	53, 269, 297, 655, 1118, 1390, 1568
MalI	GATC	6 cut(s)	99, 852, 1003, 1094, 1422, 1506
MboI	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
MboII	GAAGA	6 cut(s)	110, 529, 696, 1202, 1482, 1514
MfeI	CAATTG	2 cut(s)	672, 1209
MflI	RGATCY	1 cut(s)	1001
MhlI	GDGCHC	1 cut(s)	568
MluI	ACGCGT	1 cut(s)	1150
MmeI	TCCRAC	4 cut(s)	156, 291, 334, 1161
Mph1103I	ATGCAT	1 cut(s)	1342
MroXI	GAANNNNTTC	1 cut(s)	990
MseI	TTAA	8 cut(s)	14, 62, 206, 215, 720, 818, 1316, 1379
MspA1I	CMGCKG	2 cut(s)	103, 1534
MspCI	CTTAAG	1 cut(s)	205
MspI	CCGG	2 cut(s)	28, 898
MspR9I	CCNGG	6 cut(s)	28, 29, 158, 462, 1061, 1301
MunI	CAATTG	2 cut(s)	672, 1209
Mva1269I	GAATGC	1 cut(s)	381
MvaI	CCWGG	4 cut(s)	158, 462, 1061, 1301
MvnI	CGCG	1 cut(s)	1152
MwoI	GCNNNNNNNGC	2 cut(s)	100, 325
NciI	CCSGG	2 cut(s)	28, 29
NdeII	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
NlaIII	CATG	7 cut(s)	369, 589, 632, 689, 1079, 1555, 1563
NlaIV	GGNNCC	3 cut(s)	902, 1239, 1298
NmuCI	GTSAC	4 cut(s)	53, 297, 1390, 1568
NsiI	ATGCAT	1 cut(s)	1342
PagI	TCATGA	2 cut(s)	1075, 1551
PasI	CCCWGGG	1 cut(s)	461
PceI	AGGCCT	1 cut(s)	1059
PciSI	GCTCTTC	1 cut(s)	123
PctI	GAATGC	1 cut(s)	381
PdmI	GAANNNNTTC	1 cut(s)	990
PfeI	GAWTC	4 cut(s)	467, 1193, 1541, 1580
PflMI	CCANNNNNTGG	2 cut(s)	86, 371
PinAI	ACCGGT	1 cut(s)	897
PkrI	GCNGC	3 cut(s)	1011, 1051, 1536
PpuMI	RGGWCCY	1 cut(s)	1297
PsiI	TTATAA	1 cut(s)	612
Psp5II	RGGWCCY	1 cut(s)	1297
Psp6I	CCWGG	4 cut(s)	156, 460, 1059, 1299
PspGI	CCWGG	4 cut(s)	156, 460, 1059, 1299
PspN4I	GGNNCC	3 cut(s)	902, 1239, 1298
PspPI	GGNCC	4 cut(s)	356, 1297, 1357, 1528
PspPPI	RGGWCCY	1 cut(s)	1297
PstNI	CAGNNNCTG	1 cut(s)	557
PsuI	RGATCY	1 cut(s)	1001
PvuII	CAGCTG	2 cut(s)	103, 1534
RsaI	GTAC	2 cut(s)	782, 902
RsaNI	GTAC	2 cut(s)	781, 901
SapI	GCTCTTC	1 cut(s)	123
SaqAI	TTAA	8 cut(s)	14, 62, 206, 215, 720, 818, 1316, 1379
SatI	GCNGC	3 cut(s)	1010, 1050, 1535
Sau3AI	GATC	6 cut(s)	97, 850, 1001, 1092, 1420, 1504
Sau96I	GGNCC	4 cut(s)	356, 1297, 1357, 1528
ScrFI	CCNGG	6 cut(s)	28, 29, 158, 462, 1061, 1301
SduI	GDGCHC	1 cut(s)	568
SfaNI	GCATC	6 cut(s)	557, 677, 1012, 1081, 1327, 1349
SinI	GGWCC	3 cut(s)	356, 1297, 1528
SmaI	CCCGGG	1 cut(s)	29
SmlI	CTYRAG	2 cut(s)	205, 1142
SmoI	CTYRAG	2 cut(s)	205, 1142
SseBI	AGGCCT	1 cut(s)	1059
SsiI	CCGC	1 cut(s)	1217
SspI	AATATT	3 cut(s)	12, 289, 1036
SspMI	CTAG	6 cut(s)	95, 302, 872, 905, 1089, 1566
StuI	AGGCCT	1 cut(s)	1059
StyD4I	CCNGG	6 cut(s)	26, 27, 156, 460, 1059, 1299
StyI	CCWWGG	2 cut(s)	85, 1088
TaaI	ACNGT	6 cut(s)	174, 559, 635, 778, 1179, 1428
TaqI	TCGA	3 cut(s)	861, 1102, 1507
TatI	WGTACW	1 cut(s)	780
TfiI	GAWTC	4 cut(s)	467, 1193, 1541, 1580
Tru1I	TTAA	8 cut(s)	14, 62, 206, 215, 720, 818, 1316, 1379
Tru9I	TTAA	8 cut(s)	14, 62, 206, 215, 720, 818, 1316, 1379
TscAI	CASTG	4 cut(s)	286, 550, 783, 1433
TseFI	GTSAC	4 cut(s)	53, 297, 1390, 1568
TseI	GCWGC	3 cut(s)	1009, 1049, 1534
Tsp45I	GTSAC	4 cut(s)	53, 297, 1390, 1568
TspDTI	ATGAA	5 cut(s)	110, 674, 759, 851, 999
TspMI	CCCGGG	1 cut(s)	27
TspRI	CASTG	4 cut(s)	286, 550, 783, 1433
Van91I	CCANNNNNTGG	2 cut(s)	86, 371
Vha464I	CTTAAG	1 cut(s)	205
VneI	GTGCAC	1 cut(s)	564
VpaK11BI	GGWCC	3 cut(s)	356, 1297, 1528
XapI	RAATTY	5 cut(s)	251, 475, 876, 910, 1381
XmaI	CCCGGG	1 cut(s)	27
XmaJI	CCTAGG	1 cut(s)	1088
XmnI	GAANNNNTTC	1 cut(s)	990
XspI	CTAG	6 cut(s)	95, 302, 872, 905, 1089, 1566
Zsp2I	ATGCAT	1 cut(s)	1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.