Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0008055

Physical Location & Seq

Genomic Coordinates Forward (+)

21958792 .. 21964867

Gene Structure 6076 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1104600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3075 bp

ATGTTGGAGGGTAATCATGCAGCCTTTTCAAAGGAAATGCAGTCGCCGCGTTTTCAGGCAATACTGCGTGTCACGAGTGGAAGAAAGAAGAGAGCACCTGAAATTAAGAGTTTCTCTCATGAGCTCAACTCCAAAGGAGTTCGACCATTTCCTGTGTGGAAGTCTCGTGCATTCGGGCATATGGAGGAGATTATGGTGGCGGTCAAGGCAAGATTTGAGAAGTTAAAGGAAGAGGTTGACTTTGATTTAGGTGCATTTGCTGGTGATTTGGTCGGAATGCTTGAAAAGTCTACACAGTCTCATCCTGAATGGAAGGAGAACTTTGAGGATTTGTTGGTTATTGCTCGGAGGTGTGCAAAGATGTCTCCAAGTGAATTTTGGGTCAAGTGTGAAGGCATTGTTCAGAATTTGGATGACCGGCGTCAAGAGATACCAATGGGGGCCCTCAAACAAGCCCACACCCGTCTTCTTTTTATTCTCACTCGGTGCACACGGCTTGTGCAGTTCCAGAAAGAGAGTGGCTATGAGGAAGAGCACATTCTCAGTCTTCATCAGTTCAGTGATCTTGGAGTTTATCCAGAACAAGTTGTGGAGGCTACACAGCAGAGCTATAGTGGTTCACTGGGTGGGAAGGAAGCGAATGAGAAGAAAATGAGGAAGTCTCGTGAGCAGGAAAAAAATTGTGGAGTTGAGGAGGTGGAGGTTGGTACTGCCAAAAGCGTTGAATCTACTGGCAGCTATAGGATGTCATCTTGGAAAAAACTTCCCTCTGCTGCTGAGAAAAATCAGAAAGGCAATGATGTTGCTGACACACCGTCAAAGGAAAAGTCAGATCGTTTGCATGCCAAAGATGACACAAAAACTTGTGGTGACTACAGTACTGAACATCTGGATACTTCATCATGCCATCTTGAACACTCAGAGGTGTCTGCAAGTGCACAAACTGTTTCTTGGGGAATATGGATGGACCAGCAGAATGTTGCATACGAAAATTTGATGATCTGTCGCATATGTGAGGTTGAAATTCCAACGGTACATGTAGAGGAACACTCTCGAATATGTACAATTGCTGATAGGTGTGACTTGAAGGGTTTAACTGTGAATGAACGACTTGAAAGAGTTGCTGAAGCTCTTGAAAGGATAATGGAATCTTGGACACCAAAAGGCATTGAAACTCGAGGAAATTTTGATGTTGCAAGAGTGTATCCATCACAGATGCATGAGGATTCAAATGAGTTGTCACCAAAGAGAATTGACTTGTCTCGGTTCTCTGAAAGCATTCTTGATTGCATTCCTGATGTTGACAACTCTTTTGTTATGGAAGATCTGAATGTTTTGCCGGACATCTCATGTGATGCACATTCTGCTTTGATACGTGAACAAGGCACCAGAACCTCGTCAGCAGGTAGTTCGACACCAAGATCACCATTACAAACACCAAGAACCAGTCATCTTGAAATGCTGCTGAGTGGAGGGAGAGCAATACCGGAACTAGAGAGTTCTCAGCAGATACACAAGCTACTGGACATCGCTCGTTCTGTTACAAATGTTAGCAATTGTGACTACACTGCATTGGAGTATATGCTTGAACGACTGGAAGACCTAAAATATGCTATCCAGGACAGGAAGGTGGATGCACTCGTTGTAGAGACTTTTGGAAGGCGTATTGAAAAATTATTGCAGGAAAAATATGTACAGCTTTGTGGGCAGATAGAAGATGAAAAATTGGATTCATCAAATGGTATGGCTGATGAAGAAAGTTCGGTGGAAGATGAAGCAGTTCGTAGTCTGCGTGTCAGCCCCATCAATCCGTGTTCTAAGGATCGAACTTCTATTGAAGATTTTGAGATAATAAAACCAATAAGCAGGGGTGCATTTGGACGAGTTTTTCTTGCCAGAAAAAGAGCGACTGGTGACTTATTTGCTATAAAGGTATTAAAGAAGGCTGATATGATCCGTAAAAATGCCGTTGAGAGTATTTTGGCAGAGCGTAACATCCTTATATTGGTTCGCAATCCCTTTGTGGTCCGATTTTTCTATTCTTTCACATGCAGGGAAAATCTTTATCTGGTCATGGAGTACTTAAATGGTGGAGATCTTTACTCTTTATTGAGAAATCTAGGCTGCTTGGATGAAGATATGGCCCGCGTATATATTGCAGAAGTTGTTCTTGCTTTGGAGTATTTGCATTCATTAAATGTTATCCATAGAGACTTGAAGCCAGACAACCTGTTGATTGGTCAAGATGGTCATATCAAGTTGACAGATTTTGGGCTCTCCAAGGTTGGTCTAATCAGCAGCACTGACGACTTATCAGGTCCATCAGTTAGTGGTACCGGTTTTGTCCCAGATGATGAACCAAAATCTGAGTCTTCATCAGAAAGGGCACAGCGCCAAAAACATTCAATCGTTGGGACCCCTGATTATTTGGCACCTGAAATACTTCTTGGCATGGGGCACAGTGCAACTGCTGACTGGTGGTCTGTGGGGGTTATTCTTTTTGAGGTGCTTGTCGGTATTCCACCATTCAATGCAGAACATCCACAGCAAATTTTCAACAATATAATAAACAGAGATATCCCCTGGCCTAAGGTACCCGAGGAGATGAGCCCTGAAGCGTACGATTTAATTGACAAACTGCTGACCGACAACCCAGTTCAAAGACTTGGAGCAACAGGTACTAGGGAGGTGAAGCAACATCCTTTTTTCAAGGATATTAACTGGGATACCCTTGCGAGGCAGAAGGCTATGTTCATACCATCAACCGATATACACGATACAAGTTATTTCATGAGCCGGTACATATGGAATCCAGAAGATGATCATGTTAATGGTGCCAGTGACTTTGATGACATGACTGAAACATGCAGCAGCGGTTCATGTAGCAACATACAAGACGAAGATGGGGATGAATGTGGTAGTTTGGCGGACTTCAGTGCGCCAAACCTTGACGTGCAGTACTCTTTCAGTAATTTTTCATTCAAGAACTTATCGCAGCTTGCTTCAATCAATTATGATCTTCTCGTGAAGAACACCAAGGAGTCACCGGGTGCCTCCAAGTCATCTGTACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1025

Amino Acids

115.29

Weight (kDa)

5.17

Isoelectric Point (pI)

44.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	53 - 171	1.1e-55	IREH1-like domain
Pkinase	PF00069	615 - 903	6.2e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	616 - 762	8.2e-25	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	796 - 889	2.3e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62310
fragaria_vesca	FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920
malus_domestica	MD00G1104600.v1.1 MD07G1256300.v1.1
prunus_persica	Prupe.2G255800_v2.0.a1 Prupe.2G280200_v2.0.a1 Prupe.2G280200_v2.0.a1
pyrus_communis	pycom01g19590 pycom07g22920
rosa_chinensis	RchiOBHm_Chr1g0374881
rosa_laevigata	RLG00000026710
rosa_multiflora	Rmu_sc0003145.1_g000005 Rmu_sc0004947.1_g000030
rosa_roxburghii	Rroxscaffold_4G00283210
rosa_rugosa	Rorug01G0385500
rosa_samantha	Rh1AG397100 Rh1BG360900 Rh1CG374600 Rh1DG391900
rosa_wichuraiana	Rw1G035210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1394
Acc65I	GGTACC	2 cut(s)	2333, 2593
AccB1I	GGYRCC	6 cut(s)	1385, 2333, 2431, 2593, 2834, 3050
AccI	GTMKAC	1 cut(s)	290
AccII	CGCG	2 cut(s)	49, 2148
AciI	CCGC	5 cut(s)	47, 200, 2146, 2874, 2927
AclWI	GGATC	2 cut(s)	1830, 1948
AcsI	RAATTY	6 cut(s)	374, 406, 991, 1023, 1183, 2550
AcuI	CTGAAG	3 cut(s)	1146, 2634, 2917
AcyI	GRCGYC	1 cut(s)	421
AdeI	CACNNNGTG	3 cut(s)	486, 626, 3050
AfiI	CCNNNNNNNGG	2 cut(s)	2005, 2736
AflIII	ACRYGT	1 cut(s)	1036
AgeI	ACCGGT	1 cut(s)	2336
AhdI	GACNNNNNGTC	2 cut(s)	814, 2479
AjiI	CACGTC	1 cut(s)	2953
AjnI	CCWGG	2 cut(s)	1617, 2582
AjuI	GAANNNNNNNTTGG	2 cut(s)	2430, 2462
AluBI	AGCT	7 cut(s)	124, 609, 738, 1130, 1519, 1699, 2998
AluI	AGCT	7 cut(s)	124, 609, 738, 1130, 1519, 1699, 2998
Alw21I	GWGCWC	5 cut(s)	97, 126, 491, 537, 940
Alw26I	GTCTC	7 cut(s)	168, 303, 369, 666, 1266, 1643, 2205
Alw44I	GTGCAC	2 cut(s)	487, 936
AlwI	GGATC	2 cut(s)	1830, 1948
Ama87I	CYCGRG	2 cut(s)	1176, 2597
AoxI	GGCC	3 cut(s)	441, 2142, 2585
ApaI	GGGCCC	1 cut(s)	445
ApaLI	GTGCAC	2 cut(s)	487, 936
ApeKI	GCWGC	9 cut(s)	20, 735, 773, 1462, 2124, 2298, 2868, 2871, 2995
ApoI	RAATTY	6 cut(s)	374, 406, 991, 1023, 1183, 2550
ArsI	GACNNNNNNTTYG	8 cut(s)	812, 844, 1223, 1255, 1295, 1327, 2354, 2386
AsiGI	ACCGGT	1 cut(s)	2336
Asp700I	GAANNNNTTC	4 cut(s)	1278, 1779, 2166, 2442
Asp718I	GGTACC	2 cut(s)	2333, 2593
AspLEI	GCGC	2 cut(s)	2394, 2941
AspS9I	GGNCC	7 cut(s)	441, 442, 967, 2026, 2143, 2318, 2415
AsuC2I	CCSGG	1 cut(s)	3048
AsuHPI	GGTGA	7 cut(s)	275, 881, 1233, 1416, 1925, 2701, 3036
AvaI	CYCGRG	2 cut(s)	1176, 2597
AvaII	GGWCC	4 cut(s)	967, 2026, 2318, 2415
AxyI	CCTNAGG	1 cut(s)	2589
BaeGI	GKGCMC	5 cut(s)	445, 491, 940, 2389, 2460
BanI	GGYRCC	6 cut(s)	1385, 2333, 2431, 2593, 2834, 3050
BanII	GRGCYC	4 cut(s)	126, 445, 2277, 2612
BauI	CACGAG	4 cut(s)	73, 165, 663, 3023
BbsI	GAAGAC	4 cut(s)	458, 539, 1605, 2364
Bbv12I	GWGCWC	5 cut(s)	97, 126, 491, 537, 940
BbvI	GCAGC	9 cut(s)	32, 747, 760, 1449, 2111, 2310, 2880, 2883, 3007
BccI	CCATC	8 cut(s)	915, 958, 1216, 1811, 2240, 2329, 2767, 2897
BceAI	ACGGC	2 cut(s)	509, 1952
BcgI	CGANNNNNNTGC	2 cut(s)	1392, 1426
BciT130I	CCWGG	2 cut(s)	1619, 2584
BciVI	GTATCC	3 cut(s)	886, 1215, 2719
BclI	TGATCA	1 cut(s)	2821
BcnI	CCSGG	1 cut(s)	3048
BcoDI	GTCTC	7 cut(s)	168, 303, 369, 666, 1266, 1643, 2205
BfaI	CTAG	3 cut(s)	1493, 2120, 2682
BfmI	CTRYAG	3 cut(s)	610, 739, 874
BfoI	RGCGCY	1 cut(s)	2395
BfuAI	ACCTGC	1 cut(s)	1394
BfuI	GTATCC	3 cut(s)	886, 1215, 2719
BglII	AGATCT	2 cut(s)	1324, 2095
BmcAI	AGTACT	3 cut(s)	880, 2081, 2960
Bme1390I	CCNGG	3 cut(s)	1619, 2584, 3048
Bme18I	GGWCC	4 cut(s)	967, 2026, 2318, 2415
BmeRI	GACNNNNNGTC	2 cut(s)	814, 2479
BmeT110I	CYCGRG	2 cut(s)	1176, 2597
BmgBI	CACGTC	1 cut(s)	2953
BmgT120I	GGNCC	7 cut(s)	441, 442, 967, 2026, 2143, 2318, 2415
BmrFI	CCNGG	3 cut(s)	1619, 2584, 3048
BmrI	ACTGGG	3 cut(s)	632, 2648, 2731
BmsI	GCATC	3 cut(s)	1206, 1345, 1624
BmuI	ACTGGG	3 cut(s)	632, 2648, 2731
BoxI	GACNNNNGTC	1 cut(s)	420
BpiI	GAAGAC	4 cut(s)	458, 539, 1605, 2364
BplI	GAGNNNNNCTC	8 cut(s)	100, 132, 113, 145, 646, 678, 1034, 1066
BpuMI	CCSGG	1 cut(s)	3048
BsaAI	YACGTR	1 cut(s)	1376
BsaHI	GRCGYC	1 cut(s)	421
BsaJI	CCNNGG	4 cut(s)	2280, 2582, 2598, 3036
BsaWI	WCCGGW	2 cut(s)	1486, 2336
Bsc4I	CCNNNNNNNGG	2 cut(s)	2005, 2736
Bse118I	RCCGGY	3 cut(s)	417, 2336, 2796
Bse21I	CCTNAGG	1 cut(s)	2589
Bse3DI	GCAATG	1 cut(s)	802
BseBI	CCWGG	2 cut(s)	1619, 2584
BseDI	CCNNGG	4 cut(s)	2280, 2582, 2598, 3036
BseLI	CCNNNNNNNGG	2 cut(s)	2005, 2736
BseMI	GCAATG	1 cut(s)	802
BseMII	CTCAG	6 cut(s)	556, 768, 933, 1457, 1517, 2358
BseRI	GAGGAG	3 cut(s)	200, 707, 2615
BseSI	GKGCMC	5 cut(s)	445, 491, 940, 2389, 2460
BseXI	GCAGC	9 cut(s)	32, 747, 760, 1449, 2111, 2310, 2880, 2883, 3007
BsgI	GTGCAG	2 cut(s)	521, 2975
Bsh1236I	CGCG	2 cut(s)	49, 2148
BshFI	GGCC	3 cut(s)	443, 2144, 2587
BshNI	GGYRCC	6 cut(s)	1385, 2333, 2431, 2593, 2834, 3050
BshTI	ACCGGT	1 cut(s)	2336
BsiHKAI	GWGCWC	5 cut(s)	97, 126, 491, 537, 940
BsiHKCI	CYCGRG	2 cut(s)	1176, 2597
BsiSI	CCGG	6 cut(s)	418, 1340, 1487, 2337, 2797, 3047
BsiWI	CGTACG	1 cut(s)	2619
BslFI	GGGAC	2 cut(s)	2330, 2428
BslI	CCNNNNNNNGG	2 cut(s)	2005, 2736
BsmAI	GTCTC	7 cut(s)	168, 303, 369, 666, 1266, 1643, 2205
BsmFI	GGGAC	2 cut(s)	2330, 2428
BsmI	GAATGC	5 cut(s)	170, 282, 1278, 1290, 2188
BsnI	GGCC	3 cut(s)	443, 2144, 2587
BsoBI	CYCGRG	2 cut(s)	1176, 2597
Bsp120I	GGGCCC	1 cut(s)	441
Bsp1407I	TGTACA	2 cut(s)	1061, 1693
BspACI	CCGC	5 cut(s)	47, 200, 2146, 2874, 2927
BspANI	GGCC	3 cut(s)	443, 2144, 2587
BspCNI	CTCAG	6 cut(s)	555, 769, 932, 1458, 1516, 2359
BspFNI	CGCG	2 cut(s)	49, 2148
BspHI	TCATGA	2 cut(s)	118, 2790
BspMI	ACCTGC	1 cut(s)	1394
BspPI	GGATC	2 cut(s)	1830, 1948
BspQI	GCTCTTC	1 cut(s)	525
BspT107I	GGYRCC	6 cut(s)	1385, 2333, 2431, 2593, 2834, 3050
BsrDI	GCAATG	1 cut(s)	802
BsrFI	RCCGGY	3 cut(s)	417, 2336, 2796
BsrGI	TGTACA	2 cut(s)	1061, 1693
BssAI	RCCGGY	3 cut(s)	417, 2336, 2796
BssECI	CCNNGG	4 cut(s)	2280, 2582, 2598, 3036
BssNI	GRCGYC	1 cut(s)	421
BssSI	CACGAG	4 cut(s)	73, 165, 663, 3023
BssT1I	CCWWGG	2 cut(s)	2280, 3036
Bst2BI	CACGAG	4 cut(s)	73, 165, 663, 3023
Bst2UI	CCWGG	2 cut(s)	1619, 2584
Bst4CI	ACNGT	8 cut(s)	297, 816, 878, 946, 1033, 1099, 2462, 3072
Bst6I	CTCTTC	3 cut(s)	83, 225, 525
BstACI	GRCGYC	1 cut(s)	421
BstAPI	GCANNNNNTGC	1 cut(s)	1364
BstAUI	TGTACA	2 cut(s)	1061, 1693
BstBAI	YACGTR	1 cut(s)	1376
BstC8I	GCNNGC	3 cut(s)	843, 2146, 3000
BstDEI	CTNAG	8 cut(s)	542, 777, 919, 1466, 1503, 1818, 2367, 2589
BstFNI	CGCG	2 cut(s)	49, 2148
BstH2I	RGCGCY	1 cut(s)	2395
BstHHI	GCGC	2 cut(s)	2394, 2941
BstMAI	GTCTC	7 cut(s)	168, 303, 369, 666, 1266, 1643, 2205
BstMWI	GCNNNNNNNGC	4 cut(s)	46, 206, 1364, 1705
BstNI	CCWGG	2 cut(s)	1619, 2584
BstNSI	RCATGY	4 cut(s)	845, 1040, 2052, 2868
BstPAI	GACNNNNGTC	1 cut(s)	420
BstSCI	CCNGG	3 cut(s)	1617, 2582, 3046
BstSFI	CTRYAG	3 cut(s)	610, 739, 874
BstSLI	GKGCMC	5 cut(s)	445, 491, 940, 2389, 2460
BstUI	CGCG	2 cut(s)	49, 2148
BstV1I	GCAGC	9 cut(s)	32, 747, 760, 1449, 2111, 2310, 2880, 2883, 3007
BstV2I	GAAGAC	4 cut(s)	458, 539, 1605, 2364
BstX2I	RGATCY	2 cut(s)	1324, 2095
BstYI	RGATCY	2 cut(s)	1324, 2095
Bsu36I	CCTNAGG	1 cut(s)	2589
BsuI	GTATCC	3 cut(s)	886, 1215, 2719
BsuRI	GGCC	3 cut(s)	443, 2144, 2587
BtgZI	GCGATG	1 cut(s)	1513
BtrI	CACGTC	1 cut(s)	2953
BtsI	GCAGTG	1 cut(s)	1566
BtsIMutI	CAGTG	7 cut(s)	565, 620, 1566, 2301, 2467, 2845, 2941
BveI	ACCTGC	1 cut(s)	1394
Cac8I	GCNNGC	3 cut(s)	843, 2146, 3000
CciI	TCATGA	2 cut(s)	118, 2790
CfoI	GCGC	2 cut(s)	2394, 2941
Cfr10I	RCCGGY	3 cut(s)	417, 2336, 2796
Cfr13I	GGNCC	7 cut(s)	441, 442, 967, 2026, 2143, 2318, 2415
CseI	GACGC	1 cut(s)	410
CspAI	ACCGGT	1 cut(s)	2336
DdeI	CTNAG	8 cut(s)	542, 777, 919, 1466, 1503, 1818, 2367, 2589
DraIII	CACNNNGTG	3 cut(s)	486, 626, 3050
DriI	GACNNNNNGTC	2 cut(s)	814, 2479
Eam1104I	CTCTTC	3 cut(s)	83, 225, 525
Eam1105I	GACNNNNNGTC	2 cut(s)	814, 2479
EarI	CTCTTC	3 cut(s)	83, 225, 525
EciI	GGCGGA	1 cut(s)	2942
Ecl136II	GAGCTC	1 cut(s)	124
Eco130I	CCWWGG	2 cut(s)	2280, 3036
Eco24I	GRGCYC	4 cut(s)	126, 445, 2277, 2612
Eco32I	GATATC	1 cut(s)	2578
Eco47I	GGWCC	4 cut(s)	967, 2026, 2318, 2415
Eco53kI	GAGCTC	1 cut(s)	124
Eco57I	CTGAAG	3 cut(s)	1146, 2634, 2917
Eco81I	CCTNAGG	1 cut(s)	2589
Eco88I	CYCGRG	2 cut(s)	1176, 2597
EcoICRI	GAGCTC	1 cut(s)	124
EcoO109I	RGGNCCY	3 cut(s)	441, 442, 2415
EcoRII	CCWGG	2 cut(s)	1617, 2582
EcoRV	GATATC	1 cut(s)	2578
EcoT14I	CCWWGG	2 cut(s)	2280, 3036
EcoT22I	ATGCAT	1 cut(s)	1221
EcoT38I	GRGCYC	4 cut(s)	126, 445, 2277, 2612
ErhI	CCWWGG	2 cut(s)	2280, 3036
FalI	AAGNNNNNCTT	4 cut(s)	305, 337, 2154, 2186
FaqI	GGGAC	2 cut(s)	2330, 2428
FauI	CCCGC	1 cut(s)	2153
FauNDI	CATATG	3 cut(s)	180, 1010, 2804
FbaI	TGATCA	1 cut(s)	2821
FblI	GTMKAC	1 cut(s)	290
FriOI	GRGCYC	4 cut(s)	126, 445, 2277, 2612
FspBI	CTAG	3 cut(s)	1493, 2120, 2682
GlaI	GCGC	2 cut(s)	2393, 2940
HaeII	RGCGCY	1 cut(s)	2395
HaeIII	GGCC	3 cut(s)	443, 2144, 2587
HapII	CCGG	6 cut(s)	418, 1340, 1487, 2337, 2797, 3047
HgaI	GACGC	1 cut(s)	410
HhaI	GCGC	2 cut(s)	2394, 2941
Hin1I	GRCGYC	1 cut(s)	421
Hin6I	GCGC	2 cut(s)	2392, 2939
HinP1I	GCGC	2 cut(s)	2392, 2939
HincII	GTYRAC	3 cut(s)	238, 1303, 2262
HindII	GTYRAC	3 cut(s)	238, 1303, 2262
HinfI	GANTC	7 cut(s)	725, 1148, 1226, 1730, 2369, 2809, 3041
HpaII	CCGG	6 cut(s)	418, 1340, 1487, 2337, 2797, 3047
HphI	GGTGA	7 cut(s)	275, 881, 1233, 1416, 1925, 2701, 3036
Hpy166II	GTNNAC	8 cut(s)	238, 291, 489, 620, 938, 1303, 1379, 2262
Hpy8I	GTNNAC	8 cut(s)	238, 291, 489, 620, 938, 1303, 1379, 2262
HpyAV	CCTTC	8 cut(s)	307, 386, 625, 1081, 1621, 1653, 1936, 2737
HpyCH4III	ACNGT	8 cut(s)	297, 816, 878, 946, 1033, 1099, 2462, 3072
HpyCH4IV	ACGT	2 cut(s)	1375, 2952
HpyF10VI	GCNNNNNNNGC	4 cut(s)	46, 206, 1364, 1705
HpyF3I	CTNAG	8 cut(s)	542, 777, 919, 1466, 1503, 1818, 2367, 2589
HpySE526I	ACGT	2 cut(s)	1375, 2952
Hsp92I	GRCGYC	1 cut(s)	421
HspAI	GCGC	2 cut(s)	2392, 2939
KflI	GGGWCCC	1 cut(s)	2415
KpnI	GGTACC	2 cut(s)	2337, 2597
Ksp22I	TGATCA	1 cut(s)	2821
LguI	GCTCTTC	1 cut(s)	525
LmnI	GCTCC	1 cut(s)	2669
Lsp1109I	GCAGC	9 cut(s)	32, 747, 760, 1449, 2111, 2310, 2880, 2883, 3007
LweI	GCATC	3 cut(s)	1206, 1345, 1624
MaeI	CTAG	3 cut(s)	1493, 2120, 2682
MaeII	ACGT	2 cut(s)	1375, 2952
MfeI	CAATTG	2 cut(s)	1065, 1555
MflI	RGATCY	2 cut(s)	1324, 2095
MlyI	GAGTC	2 cut(s)	2378, 3050
MmeI	TCCRAC	2 cut(s)	253, 1052
Mph1103I	ATGCAT	1 cut(s)	1221
MroXI	GAANNNNTTC	4 cut(s)	1278, 1779, 2166, 2442
MseI	TTAA	9 cut(s)	105, 224, 1094, 1937, 2084, 2195, 2627, 2718, 2829
MslI	CAYNNNNRTG	1 cut(s)	2829
MspA1I	CMGCKG	1 cut(s)	2874
MspI	CCGG	6 cut(s)	418, 1340, 1487, 2337, 2797, 3047
MspR9I	CCNGG	3 cut(s)	1619, 2584, 3048
MunI	CAATTG	2 cut(s)	1065, 1555
Mva1269I	GAATGC	5 cut(s)	170, 282, 1278, 1290, 2188
MvaI	CCWGG	2 cut(s)	1619, 2584
MvnI	CGCG	2 cut(s)	49, 2148
MwoI	GCNNNNNNNGC	4 cut(s)	46, 206, 1364, 1705
NciI	CCSGG	1 cut(s)	3048
NdeI	CATATG	3 cut(s)	180, 1010, 2804
NmuCI	GTSAC	8 cut(s)	70, 869, 1079, 1239, 1559, 1913, 2840, 3042
NsiI	ATGCAT	1 cut(s)	1221
NspI	RCATGY	4 cut(s)	845, 1040, 2052, 2868
PaeI	GCATGC	1 cut(s)	845
PaeR7I	CTCGAG	1 cut(s)	1176
PagI	TCATGA	2 cut(s)	118, 2790
PciI	ACATGT	1 cut(s)	1036
PciSI	GCTCTTC	1 cut(s)	525
PcsI	WCGNNNNNNNCGW	1 cut(s)	1789
PctI	GAATGC	5 cut(s)	170, 282, 1278, 1290, 2188
PdmI	GAANNNNTTC	4 cut(s)	1278, 1779, 2166, 2442
PfeI	GAWTC	5 cut(s)	725, 1148, 1226, 1730, 2809
Pfl23II	CGTACG	1 cut(s)	2619
PfoI	TCCNGGA	1 cut(s)	1617
PinAI	ACCGGT	1 cut(s)	2336
PleI	GAGTC	2 cut(s)	2377, 3049
PpsI	GAGTC	2 cut(s)	2377, 3049
Ppu21I	YACGTR	1 cut(s)	1376
PpuMI	RGGWCCY	1 cut(s)	2415
PscI	ACATGT	1 cut(s)	1036
PshAI	GACNNNNGTC	1 cut(s)	420
Psp124BI	GAGCTC	1 cut(s)	126
Psp5II	RGGWCCY	1 cut(s)	2415
Psp6I	CCWGG	2 cut(s)	1617, 2582
PspGI	CCWGG	2 cut(s)	1617, 2582
PspLI	CGTACG	1 cut(s)	2619
PspOMI	GGGCCC	1 cut(s)	441
PspPI	GGNCC	7 cut(s)	441, 442, 967, 2026, 2143, 2318, 2415
PspPPI	RGGWCCY	1 cut(s)	2415
PspXI	VCTCGAGB	1 cut(s)	1176
PsuI	RGATCY	2 cut(s)	1324, 2095
RseI	CAYNNNNRTG	1 cut(s)	2829
SacI	GAGCTC	1 cut(s)	126
SapI	GCTCTTC	1 cut(s)	525
SaqAI	TTAA	9 cut(s)	105, 224, 1094, 1937, 2084, 2195, 2627, 2718, 2829
Sau96I	GGNCC	7 cut(s)	441, 442, 967, 2026, 2143, 2318, 2415
ScaI	AGTACT	3 cut(s)	880, 2081, 2960
SchI	GAGTC	2 cut(s)	2378, 3050
ScrFI	CCNGG	3 cut(s)	1619, 2584, 3048
SfaNI	GCATC	3 cut(s)	1206, 1345, 1624
SfcI	CTRYAG	3 cut(s)	610, 739, 874
Sfr274I	CTCGAG	1 cut(s)	1176
SinI	GGWCC	4 cut(s)	967, 2026, 2318, 2415
SlaI	CTCGAG	1 cut(s)	1176
SmiMI	CAYNNNNRTG	1 cut(s)	2829
SmlI	CTYRAG	1 cut(s)	1176
SmoI	CTYRAG	1 cut(s)	1176
SphI	GCATGC	1 cut(s)	845
SsiI	CCGC	5 cut(s)	47, 200, 2146, 2874, 2927
SspMI	CTAG	3 cut(s)	1493, 2120, 2682
SstI	GAGCTC	1 cut(s)	126
StyD4I	CCNGG	3 cut(s)	1617, 2582, 3046
StyI	CCWWGG	2 cut(s)	2280, 3036
TaaI	ACNGT	8 cut(s)	297, 816, 878, 946, 1033, 1099, 2462, 3072
TaiI	ACGT	2 cut(s)	1378, 2955
TaqI	TCGA	5 cut(s)	142, 1054, 1177, 1412, 1825
TaqII	GACCGA	1 cut(s)	2660
TatI	WGTACW	5 cut(s)	878, 1061, 1693, 2079, 2958
TauI	GCSGC	1 cut(s)	49
TfiI	GAWTC	5 cut(s)	725, 1148, 1226, 1730, 2809
Tru1I	TTAA	9 cut(s)	105, 224, 1094, 1937, 2084, 2195, 2627, 2718, 2829
Tru9I	TTAA	9 cut(s)	105, 224, 1094, 1937, 2084, 2195, 2627, 2718, 2829
TscAI	CASTG	7 cut(s)	565, 627, 1573, 2308, 2467, 2845, 2941
TseFI	GTSAC	8 cut(s)	70, 869, 1079, 1239, 1559, 1913, 2840, 3042
TseI	GCWGC	9 cut(s)	20, 735, 773, 1462, 2124, 2298, 2868, 2871, 2995
Tsp45I	GTSAC	8 cut(s)	70, 869, 1079, 1239, 1559, 1913, 2840, 3042
TspGWI	ACGGA	2 cut(s)	1800, 1946
TspRI	CASTG	7 cut(s)	565, 627, 1573, 2308, 2467, 2845, 2941
VneI	GTGCAC	2 cut(s)	487, 936
VpaK11BI	GGWCC	4 cut(s)	967, 2026, 2318, 2415
XapI	RAATTY	6 cut(s)	374, 406, 991, 1023, 1183, 2550
XceI	RCATGY	4 cut(s)	845, 1040, 2052, 2868
XcmI	CCANNNNNNNNNTGG	3 cut(s)	153, 375, 515
XhoI	CTCGAG	1 cut(s)	1176
XmiI	GTMKAC	1 cut(s)	290
XmnI	GAANNNNTTC	4 cut(s)	1278, 1779, 2166, 2442
XspI	CTAG	3 cut(s)	1493, 2120, 2682
ZrmI	AGTACT	3 cut(s)	880, 2081, 2960
Zsp2I	ATGCAT	1 cut(s)	1221

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.