Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008055

Physical Location & Seq

Genomic Coordinates Reverse (-)

32301319 .. 32309469

Gene Structure 8151 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1256300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3525 bp

ATGTCGAGCCCTCCTCCGCCTTCTACAAACCCGCCGCTGATTTCCATCTCCGACGGCAAATCAGACCCCGGAACGACGTCTTCCGTCTCCTCCCCGACTCAACAGCCCTCCCCCTCCAGAGCCAAGCTCCTTAAGATCCCTCAAATCCCGATTCATCGAAGCCCTAGGCACACGATCCACGAAGGCGAGGAATTCGAAGATGACGAGGAAGATGAGGACGATGATAGTGCGGAGCGCAGAGCAGACGATCCGCCTATCGTCTTGCCGTCTTCGCTCGGCCTCAATCACATTCGGACTCGGTCGGCTCCGTCGCCGCTTCGATTCTCATCCTCCGTATGCGCCGCGTTGAATTTCGGCGATGAATCGAGCAAGGACAAGTACACCGTCAAACCAAAGCCTAAACTCGCGCCCTTACAACCAGTGGAATCAATCAAAATCGCTCAGAAATGCATCACTACTCAATCCAGTGTTGGAGCCTTTGCAAAGGAAATGCAGTCGCCGCGTTTTCAGGCAATACTGCGTGTTACGAGTGGAAGAAAGAAGAGAACACCTGAAATTAAGAGTTTCTCTCATGAGCTCAACTCCAAAGGAGTTCGAGCATTTCCTGTGTGGAAGTCTCGTGCTTTCGGGCATATGGAGGAGATTATGGTGGCGCTCAAGGCAAGATTTGAGAAGTTAAAGGAAGAGGTTGACCTTGATTTAGGCGCATTTGCTGGTGATTTGGTCGGAATGCTTGAAAGGTCTACACAGTCTAATCCTGAATGGAAAGAGAACTTTGAGGATTTGTTGGTTATCGCCCGGAGGTGTGCAAAGATGTCGTCTGGTGAATTTTGGGTCAAATGTGAAGGCATTGTTCAGAGTTTGGATGACCGGCGTCAAGAGCTACCAATGGGGGCCCTCAAACAAGCGCACACCCGTCTTCTTTTTATTCTCACTCGGTGTACACGGCTTGTGCAGTTCCAGAAAGAGAGTGGCTATGAGGAAGAGCACATTCTCAGTCTTCATCAGTTCAGTGATCTTGGTGTTTATCCAGAACAAATTTTGCAGGCTGCACAGCAGAGCTATAGTGGTTCACTGGGTGGGAAGGAAATAAATGAGAAGCAAATGAAGAAGTCTCGTGAGCAGGAAAAAGTTAGTGGAGCTGAGGAAGTGGAGGTTTGCACTGCCAAAAGTGTTGAATCTACTGGCAGCTATAGGATGTCCTCTTGGAAAAAACTTCCATCTGCTGCTGAGAAAAATCAGAAAGGCAATGATGTTGCTGACACACAATCAAAGGAAAAATCAGATCGTTTGCATGCCAAAGATGACACAAAAACTTGTGGTGACTACAGTACGGAAAATTTGAGTACTTCATGCCATCCTGAACACTCAGAGGTGTCTGCAAGTGCACAAAAAGTCTCTTGGGGAATATGGATGGACCAGCAGAATGTTGCATATGAAAATGTGATGATTTGTCGAATATGTGAGGTTGAAATACCAACTGTACATGTCGAGGAACACTCTCGTATATGTACGATTGCTGATAGGTGTGACTTGAAGGGTTTAACTGTGAATGAACGACTTGAGAGAGTTGCTGAAGCTCTTGAAAGGATAATGGAATCTTGGACACCAAAAGGCACTGAAACTCGAGGAAATTATGATGCTGCAAGAGTGTATCCCTCACAGATGCATGAAGATTTAGATGAGTTGTCACCAAAGCGAATTGACTTGTCTCGATTCTCCGAAGGCAGTCTTGATTGCATTCCTGGTGTTGACAACTCTTACGTTATGGAAGATCTGAATGTTTTGCCTGACATCTCATGTGATGCACGTTCTGCTTTGATACCTGAACAAGGCACCAGAACCTCGTCAGCAGGAAGTTCGACACCAAGATCACCATTACAAACACCAAGAACCAGTCATATTGAAATGTTGCTGAGTGGAGGGAGAGCAATACCAGAACTTGAGAGTTATCAGCAGATACACAATCTACTGGACATCGCTCGTTCTGTCGCAAATGTTAGCAATTGTGACTACACTGCATTGGAGTACATGCTTGAACGACTGGAAGACCTAAAATATGCTATCCAGGACAGGAAGGTGGATGCACTCGTTGTAGAGACTTTTGGAAGGCGTATTGAAAAATTGCTGCAGGAAAAATATGTACATCTTTGTGGGCAGATAGAAGATGAAAAATTGGATTCATCAAATGGTATGGCTGATGAAGAAAGTTCAGTGGAAGATGAAGCCGTTCGTAGTCTACGTGCCAGCCCCATCAATCCATGTTCTAAGGATCGAACTTCTATTGAAGATTTTGAGACAATAAAACCAATAAGCAGGGGTGCATTTGGACGAGTTTTTCTTGCCAGAAAAAGAGCGACTGGTGACTTATTTGCTATAAAGGTATTGAAGAAGGCTGATATGATCCGTAAAAATGCCGTTGAGAGTATTTTGGCGGAGCGTAACATCCTTATATTGGTCCGCAATCCTTTTGTGGTCCGATTTTTCTATTCTTTCACCTGCACGGAAAATCTTTATCTGGTCATGGAGTACTTAAATGGTGGAGATCTTTACTCTTTATTGAGAAATCTAGGCTGCTTGGATGAAGATATGGCCCGTGTATATATTGCAGAAGTTGTTCTTGCTTTGGAGTATTTGCATTCATTAAACGTTATCCACAGAGACTTGAAGCCAGACAACCTGTTGATTGGTCAAGATGGTCATATCAAGTTGACAGATTTTGGGCTCTCCAACGTTGGTCTAATCAGCAGCACTGATGACTTATCAGGTCCATCAGTTAGTGGTACTGGTTTTGTCCCAGATGATGAACCAAAATCTGAGTCTTCATCGGAAAGGGCACAGCGCCAAAAGCATTCAGTCGTTGGGACCCCTGATTATTTGGCACCTGAAATACTTCTTGGCATGGGGCATGGTGCAACTGCTGATTGGTGGTCTGTGGGGGTTATTCTTTTTGAGCTGCTTGTCGGTATTCCACCATTCAATGCAGAACATCCACAGCAAATTTTCAACAATATAATAAACAGAGACATCCCCTGGCCTAAAGTACCTGAGGAGATGAGCCCTGAGGCGTACGATTTGATTGACAAACTGCTTACCGACAACCCAGTTCAAAGACTTGGAGCAACAGGTACCAGGGAGGTGAAGCAACATTCTTTTTTCAAGGATATTAACTGGGACACGCTTGCTAGGCAGAAGGCTATGTTCATACCATCTACCGATGCACACGATACAAGTTATTTCATGAGCCGGTACGCATGGAACCCAGAAGATGAGCATGTTAATGGCGCCAGTGACTTTGATGACATGACTGAAACATACAGCAGTGGTTCATGTAGCAACATACAAGACGAAGATGGGGATGAATGTGGTAGTTTGGTGGACTTTGGTGCTCCAACCGTTGACATGCAGTACACTTTCAGTAATTTTTCATTCAAGAATTTGTCACAGCTGGCTTCTATCAACTATGATCTTGTTGTGAAGAACGCCAAGGAGTCACCGGATGCCTCTAAGTCATCCGTGCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1175

Amino Acids

131.11

Weight (kDa)

5.13

Isoelectric Point (pI)

50.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	204 - 322	7.1e-55	IREH1-like domain
Pkinase	PF00069	765 - 1053	1.2e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	767 - 911	2.4e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62310
fragaria_vesca	FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920
malus_domestica	MD00G1104600.v1.1 MD07G1256300.v1.1
prunus_persica	Prupe.2G255800_v2.0.a1 Prupe.2G280200_v2.0.a1 Prupe.2G280200_v2.0.a1
pyrus_communis	pycom01g19590 pycom07g22920
rosa_chinensis	RchiOBHm_Chr1g0374881
rosa_laevigata	RLG00000026710
rosa_multiflora	Rmu_sc0003145.1_g000005 Rmu_sc0004947.1_g000030
rosa_roxburghii	Rroxscaffold_4G00283210
rosa_rugosa	Rorug01G0385500
rosa_samantha	Rh1AG397100 Rh1BG360900 Rh1CG374600 Rh1DG391900
rosa_wichuraiana	Rw1G035210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2507
AatII	GACGTC	1 cut(s)	80
Acc36I	ACCTGC	1 cut(s)	2507
Acc65I	GGTACC	1 cut(s)	3128
AccB1I	GGYRCC	4 cut(s)	1835, 2881, 3128, 3284
AccI	GTMKAC	2 cut(s)	743, 2239
AccII	CGCG	3 cut(s)	344, 407, 502
AclI	AACGTT	2 cut(s)	2649, 2733
AclWI	GGATC	5 cut(s)	130, 169, 242, 2280, 2398
AcsI	RAATTY	7 cut(s)	191, 349, 827, 1038, 1339, 3000, 3436
AcuI	CTGAAG	1 cut(s)	1596
AcyI	GRCGYC	3 cut(s)	77, 874, 3285
AdeI	CACNNNGTG	2 cut(s)	939, 1079
AfiI	CCNNNNNNNGG	2 cut(s)	1832, 2455
AflII	CTTAAG	1 cut(s)	131
AflIII	ACRYGT	1 cut(s)	1486
AjnI	CCWGG	4 cut(s)	1744, 2067, 3032, 3131
AjuI	GAANNNNNNNTTGG	2 cut(s)	2880, 2912
AluBI	AGCT	9 cut(s)	127, 577, 883, 1062, 1142, 1191, 1580, 2956, 3448
AluI	AGCT	9 cut(s)	127, 577, 883, 1062, 1142, 1191, 1580, 2956, 3448
Alw21I	GWGCWC	4 cut(s)	579, 990, 1390, 3391
Alw26I	GTCTC	9 cut(s)	91, 621, 1119, 1402, 1716, 2093, 2291, 2655, 3018
Alw44I	GTGCAC	1 cut(s)	1386
AlwI	GGATC	5 cut(s)	130, 169, 242, 2280, 2398
Ama87I	CYCGRG	1 cut(s)	1626
AoxI	GGCC	4 cut(s)	277, 894, 2592, 3035
ApaI	GGGCCC	1 cut(s)	898
ApaLI	GTGCAC	1 cut(s)	1386
ApeKI	GCWGC	8 cut(s)	1049, 1188, 1226, 1643, 2128, 2574, 2748, 2956
ApoI	RAATTY	7 cut(s)	191, 349, 827, 1038, 1339, 3000, 3436
ArsI	GACNNNNNNTTYG	4 cut(s)	803, 835, 2804, 2836
Asp700I	GAANNNNTTC	3 cut(s)	2229, 2616, 2892
Asp718I	GGTACC	1 cut(s)	3128
AspA2I	CCTAGG	1 cut(s)	164
AspLEI	GCGC	8 cut(s)	237, 341, 409, 655, 707, 910, 2844, 3287
AspS9I	GGNCC	8 cut(s)	894, 895, 1417, 2458, 2476, 2593, 2768, 2865
AsuC2I	CCSGG	2 cut(s)	69, 799
AsuHPI	GGTGA	9 cut(s)	728, 836, 1334, 1683, 1866, 2375, 2488, 3151, 3486
AsuII	TTCGAA	1 cut(s)	195
AvaI	CYCGRG	1 cut(s)	1626
AvaII	GGWCC	5 cut(s)	1417, 2458, 2476, 2768, 2865
AvrII	CCTAGG	1 cut(s)	164
AxyI	CCTNAGG	2 cut(s)	3048, 3063
BaeGI	GKGCMC	3 cut(s)	898, 1390, 2839
BanI	GGYRCC	4 cut(s)	1835, 2881, 3128, 3284
BanII	GRGCYC	5 cut(s)	11, 579, 898, 2727, 3062
BauI	CACGAG	2 cut(s)	618, 1116
BbsI	GAAGAC	6 cut(s)	72, 261, 911, 992, 2055, 2814
Bbv12I	GWGCWC	4 cut(s)	579, 990, 1390, 3391
BbvCI	CCTCAGC	1 cut(s)	1143
BbvI	GCAGC	8 cut(s)	1036, 1200, 1213, 1630, 2115, 2561, 2760, 2943
BccI	CCATC	9 cut(s)	53, 1228, 1365, 1408, 2261, 2690, 2779, 3217, 3347
BceAI	ACGGC	6 cut(s)	70, 250, 962, 2213, 2402, 3505
BcgI	CGANNNNNNTGC	6 cut(s)	209, 243, 798, 832, 1842, 1876
BciT130I	CCWGG	4 cut(s)	1746, 2069, 3034, 3133
BciVI	GTATCC	1 cut(s)	1665
BcnI	CCSGG	2 cut(s)	69, 799
BcoDI	GTCTC	9 cut(s)	91, 621, 1119, 1402, 1716, 2093, 2291, 2655, 3018
BfaI	CTAG	3 cut(s)	165, 2570, 3186
BfmI	CTRYAG	4 cut(s)	1063, 1192, 1327, 2129
BfoI	RGCGCY	3 cut(s)	656, 2845, 3288
BfrI	CTTAAG	1 cut(s)	131
BfuAI	ACCTGC	1 cut(s)	2507
BfuI	GTATCC	1 cut(s)	1665
BglII	AGATCT	2 cut(s)	1774, 2545
BlnI	CCTAGG	1 cut(s)	164
BmcAI	AGTACT	2 cut(s)	1348, 2531
Bme1390I	CCNGG	6 cut(s)	69, 799, 1746, 2069, 3034, 3133
Bme18I	GGWCC	5 cut(s)	1417, 2458, 2476, 2768, 2865
BmeT110I	CYCGRG	1 cut(s)	1626
BmgT120I	GGNCC	8 cut(s)	894, 895, 1417, 2458, 2476, 2593, 2768, 2865
BmrFI	CCNGG	6 cut(s)	69, 799, 1746, 2069, 3034, 3133
BmrI	ACTGGG	3 cut(s)	1085, 3098, 3181
BmsI	GCATC	7 cut(s)	459, 1630, 1656, 1795, 2074, 3208, 3490
BmuI	ACTGGG	3 cut(s)	1085, 3098, 3181
BoxI	GACNNNNGTC	1 cut(s)	873
BpiI	GAAGAC	6 cut(s)	72, 261, 911, 992, 2055, 2814
BplI	GAGNNNNNCTC	9 cut(s)	30, 111, 143, 553, 585, 566, 598, 1484, 1516
BpmI	CTGGAG	1 cut(s)	100
Bpu10I	CCTNAGC	1 cut(s)	1143
Bpu14I	TTCGAA	1 cut(s)	195
BpuEI	CTTGAG	3 cut(s)	641, 1583, 1964
BpuMI	CCSGG	2 cut(s)	69, 799
BsaAI	YACGTR	1 cut(s)	2243
BsaBI	GATNNNNATC	2 cut(s)	44, 325
BsaHI	GRCGYC	3 cut(s)	77, 874, 3285
BsaJI	CCNNGG	5 cut(s)	67, 164, 3032, 3132, 3486
BsaWI	WCCGGW	1 cut(s)	3496
Bsc4I	CCNNNNNNNGG	2 cut(s)	1832, 2455
Bse118I	RCCGGY	2 cut(s)	870, 3246
Bse21I	CCTNAGG	2 cut(s)	3048, 3063
Bse3DI	GCAATG	1 cut(s)	1255
Bse8I	GATNNNNATC	2 cut(s)	44, 325
BseBI	CCWGG	4 cut(s)	1746, 2069, 3034, 3133
BseDI	CCNNGG	5 cut(s)	67, 164, 3032, 3132, 3486
BseJI	GATNNNNATC	2 cut(s)	44, 325
BseLI	CCNNNNNNNGG	2 cut(s)	1832, 2455
BseMI	GCAATG	1 cut(s)	1255
BseMII	CTCAG	9 cut(s)	455, 1009, 1134, 1221, 1383, 1907, 2808, 3039, 3054
BseRI	GAGGAG	3 cut(s)	79, 653, 3065
BseSI	GKGCMC	3 cut(s)	898, 1390, 2839
BseXI	GCAGC	8 cut(s)	1036, 1200, 1213, 1630, 2115, 2561, 2760, 2943
BsgI	GTGCAG	3 cut(s)	974, 1035, 2485
Bsh1236I	CGCG	3 cut(s)	344, 407, 502
Bsh1285I	CGRYCG	1 cut(s)	302
BshFI	GGCC	4 cut(s)	279, 896, 2594, 3037
BshNI	GGYRCC	4 cut(s)	1835, 2881, 3128, 3284
BsiEI	CGRYCG	1 cut(s)	302
BsiHKAI	GWGCWC	4 cut(s)	579, 990, 1390, 3391
BsiHKCI	CYCGRG	1 cut(s)	1626
BsiSI	CCGG	5 cut(s)	69, 799, 871, 3247, 3497
BsiWI	CGTACG	1 cut(s)	3069
BslFI	GGGAC	3 cut(s)	2780, 2878, 3188
BslI	CCNNNNNNNGG	2 cut(s)	1832, 2455
BsmAI	GTCTC	9 cut(s)	91, 621, 1119, 1402, 1716, 2093, 2291, 2655, 3018
BsmBI	CGTCTC	1 cut(s)	91
BsmFI	GGGAC	3 cut(s)	2780, 2878, 3188
BsmI	GAATGC	4 cut(s)	735, 1740, 2638, 2851
BsnI	GGCC	4 cut(s)	279, 896, 2594, 3037
BsoBI	CYCGRG	1 cut(s)	1626
Bsp119I	TTCGAA	1 cut(s)	195
Bsp120I	GGGCCC	1 cut(s)	894
Bsp1286I	GDGCHC	9 cut(s)	11, 579, 898, 990, 1390, 2727, 2839, 3062, 3391
Bsp1407I	TGTACA	3 cut(s)	941, 1483, 2143
BspANI	GGCC	4 cut(s)	279, 896, 2594, 3037
BspCNI	CTCAG	9 cut(s)	454, 1008, 1135, 1222, 1382, 1908, 2809, 3040, 3055
BspFNI	CGCG	3 cut(s)	344, 407, 502
BspHI	TCATGA	2 cut(s)	571, 3240
BspMAI	CTGCAG	1 cut(s)	2133
BspMI	ACCTGC	1 cut(s)	2507
BspPI	GGATC	5 cut(s)	130, 169, 242, 2280, 2398
BspQI	GCTCTTC	1 cut(s)	978
BspT104I	TTCGAA	1 cut(s)	195
BspT107I	GGYRCC	4 cut(s)	1835, 2881, 3128, 3284
BspTI	CTTAAG	1 cut(s)	131
BsrDI	GCAATG	1 cut(s)	1255
BsrFI	RCCGGY	2 cut(s)	870, 3246
BsrGI	TGTACA	3 cut(s)	941, 1483, 2143
BssAI	RCCGGY	2 cut(s)	870, 3246
BssECI	CCNNGG	5 cut(s)	67, 164, 3032, 3132, 3486
BssNI	GRCGYC	3 cut(s)	77, 874, 3285
BssSI	CACGAG	2 cut(s)	618, 1116
BssT1I	CCWWGG	2 cut(s)	164, 3486
Bst2BI	CACGAG	2 cut(s)	618, 1116
Bst2UI	CCWGG	4 cut(s)	1746, 2069, 3034, 3133
Bst4CI	ACNGT	6 cut(s)	385, 750, 1331, 1483, 1549, 3397
Bst6I	CTCTTC	3 cut(s)	536, 678, 978
BstACI	GRCGYC	3 cut(s)	77, 874, 3285
BstAFI	CTTAAG	1 cut(s)	131
BstAPI	GCANNNNNTGC	1 cut(s)	1814
BstAUI	TGTACA	3 cut(s)	941, 1483, 2143
BstBAI	YACGTR	1 cut(s)	2243
BstBI	TTCGAA	1 cut(s)	195
BstC8I	GCNNGC	5 cut(s)	1047, 1296, 2248, 3183, 3450
BstENI	CCTNNNNNAGG	1 cut(s)	1830
BstFNI	CGCG	3 cut(s)	344, 407, 502
BstH2I	RGCGCY	3 cut(s)	656, 2845, 3288
BstHHI	GCGC	8 cut(s)	237, 341, 409, 655, 707, 910, 2844, 3287
BstMAI	GTCTC	9 cut(s)	91, 621, 1119, 1402, 1716, 2093, 2291, 2655, 3018
BstMCI	CGRYCG	1 cut(s)	302
BstMWI	GCNNNNNNNGC	7 cut(s)	271, 499, 659, 880, 1814, 2848, 3187
BstNI	CCWGG	4 cut(s)	1746, 2069, 3034, 3133
BstNSI	RCATGY	5 cut(s)	1298, 1490, 2035, 3278, 3406
BstPAI	GACNNNNGTC	1 cut(s)	873
BstSCI	CCNGG	6 cut(s)	67, 797, 1744, 2067, 3032, 3131
BstSFI	CTRYAG	4 cut(s)	1063, 1192, 1327, 2129
BstSLI	GKGCMC	3 cut(s)	898, 1390, 2839
BstUI	CGCG	3 cut(s)	344, 407, 502
BstV1I	GCAGC	8 cut(s)	1036, 1200, 1213, 1630, 2115, 2561, 2760, 2943
BstV2I	GAAGAC	6 cut(s)	72, 261, 911, 992, 2055, 2814
BstX2I	RGATCY	3 cut(s)	135, 1774, 2545
BstYI	RGATCY	3 cut(s)	135, 1774, 2545
Bsu36I	CCTNAGG	2 cut(s)	3048, 3063
BsuI	GTATCC	1 cut(s)	1665
BsuRI	GGCC	4 cut(s)	279, 896, 2594, 3037
BtgZI	GCGATG	2 cut(s)	372, 1963
BtsI	GCAGTG	3 cut(s)	1161, 2016, 3328
BveI	ACCTGC	1 cut(s)	2507
Cac8I	GCNNGC	5 cut(s)	1047, 1296, 2248, 3183, 3450
CciI	TCATGA	2 cut(s)	571, 3240
CfoI	GCGC	8 cut(s)	237, 341, 409, 655, 707, 910, 2844, 3287
Cfr10I	RCCGGY	2 cut(s)	870, 3246
Cfr13I	GGNCC	8 cut(s)	894, 895, 1417, 2458, 2476, 2593, 2768, 2865
CseI	GACGC	1 cut(s)	863
DinI	GGCGCC	1 cut(s)	3286
DraIII	CACNNNGTG	2 cut(s)	939, 1079
Eam1104I	CTCTTC	3 cut(s)	536, 678, 978
EarI	CTCTTC	3 cut(s)	536, 678, 978
EciI	GGCGGA	3 cut(s)	6, 240, 2450
Ecl136II	GAGCTC	1 cut(s)	577
Eco130I	CCWWGG	2 cut(s)	164, 3486
Eco24I	GRGCYC	5 cut(s)	11, 579, 898, 2727, 3062
Eco47I	GGWCC	5 cut(s)	1417, 2458, 2476, 2768, 2865
Eco53kI	GAGCTC	1 cut(s)	577
Eco57I	CTGAAG	1 cut(s)	1596
Eco81I	CCTNAGG	2 cut(s)	3048, 3063
Eco88I	CYCGRG	1 cut(s)	1626
EcoICRI	GAGCTC	1 cut(s)	577
EcoNI	CCTNNNNNAGG	1 cut(s)	1830
EcoO109I	RGGNCCY	3 cut(s)	894, 895, 2865
EcoRI	GAATTC	1 cut(s)	191
EcoRII	CCWGG	4 cut(s)	1744, 2067, 3032, 3131
EcoT14I	CCWWGG	2 cut(s)	164, 3486
EcoT22I	ATGCAT	2 cut(s)	452, 1671
EcoT38I	GRGCYC	5 cut(s)	11, 579, 898, 2727, 3062
EgeI	GGCGCC	1 cut(s)	3286
EheI	GGCGCC	1 cut(s)	3286
ErhI	CCWWGG	2 cut(s)	164, 3486
Esp3I	CGTCTC	1 cut(s)	91
FalI	AAGNNNNNCTT	4 cut(s)	1716, 1748, 2604, 2636
FaqI	GGGAC	3 cut(s)	2780, 2878, 3188
FauI	CCCGC	1 cut(s)	39
FauNDI	CATATG	2 cut(s)	633, 1435
FblI	GTMKAC	2 cut(s)	743, 2239
FriOI	GRGCYC	5 cut(s)	11, 579, 898, 2727, 3062
FspBI	CTAG	3 cut(s)	165, 2570, 3186
GlaI	GCGC	8 cut(s)	236, 340, 408, 654, 706, 909, 2843, 3286
GsuI	CTGGAG	1 cut(s)	100
HaeII	RGCGCY	3 cut(s)	656, 2845, 3288
HaeIII	GGCC	4 cut(s)	279, 896, 2594, 3037
HapII	CCGG	5 cut(s)	69, 799, 871, 3247, 3497
HgaI	GACGC	1 cut(s)	863
HhaI	GCGC	8 cut(s)	237, 341, 409, 655, 707, 910, 2844, 3287
Hin1I	GRCGYC	3 cut(s)	77, 874, 3285
Hin6I	GCGC	8 cut(s)	235, 339, 407, 653, 705, 908, 2842, 3285
HinP1I	GCGC	8 cut(s)	235, 339, 407, 653, 705, 908, 2842, 3285
HincII	GTYRAC	4 cut(s)	691, 1753, 2712, 3400
HindII	GTYRAC	4 cut(s)	691, 1753, 2712, 3400
HpaII	CCGG	5 cut(s)	69, 799, 871, 3247, 3497
HphI	GGTGA	9 cut(s)	728, 836, 1334, 1683, 1866, 2375, 2488, 3151, 3486
Hpy99I	CGWCG	3 cut(s)	56, 79, 313
HpyCH4III	ACNGT	6 cut(s)	385, 750, 1331, 1483, 1549, 3397
HpyCH4IV	ACGT	6 cut(s)	77, 1764, 1810, 2242, 2649, 2733
HpyF10VI	GCNNNNNNNGC	7 cut(s)	271, 499, 659, 880, 1814, 2848, 3187
HpySE526I	ACGT	6 cut(s)	77, 1764, 1810, 2242, 2649, 2733
Hsp92I	GRCGYC	3 cut(s)	77, 874, 3285
HspAI	GCGC	8 cut(s)	235, 339, 407, 653, 705, 908, 2842, 3285
KasI	GGCGCC	1 cut(s)	3284
KflI	GGGWCCC	1 cut(s)	2865
KpnI	GGTACC	1 cut(s)	3132
LguI	GCTCTTC	1 cut(s)	978
LmnI	GCTCC	8 cut(s)	132, 232, 310, 473, 1139, 2437, 3119, 3394
Lsp1109I	GCAGC	8 cut(s)	1036, 1200, 1213, 1630, 2115, 2561, 2760, 2943
LweI	GCATC	7 cut(s)	459, 1630, 1656, 1795, 2074, 3208, 3490
MaeI	CTAG	3 cut(s)	165, 2570, 3186
MaeII	ACGT	6 cut(s)	77, 1764, 1810, 2242, 2649, 2733
MfeI	CAATTG	1 cut(s)	2005
MflI	RGATCY	3 cut(s)	135, 1774, 2545
MhlI	GDGCHC	9 cut(s)	11, 579, 898, 990, 1390, 2727, 2839, 3062, 3391
Mly113I	GGCGCC	1 cut(s)	3285
MlyI	GAGTC	4 cut(s)	91, 289, 2828, 3500
MmeI	TCCRAC	5 cut(s)	75, 451, 706, 2754, 3416
Mph1103I	ATGCAT	2 cut(s)	452, 1671
MroXI	GAANNNNTTC	3 cut(s)	2229, 2616, 2892
MseI	TTAA	8 cut(s)	132, 558, 677, 1544, 2534, 2645, 3168, 3279
MslI	CAYNNNNRTG	2 cut(s)	2151, 3279
MspA1I	CMGCKG	2 cut(s)	37, 3448
MspCI	CTTAAG	1 cut(s)	131
MspI	CCGG	5 cut(s)	69, 799, 871, 3247, 3497
MspR9I	CCNGG	6 cut(s)	69, 799, 1746, 2069, 3034, 3133
MunI	CAATTG	1 cut(s)	2005
Mva1269I	GAATGC	4 cut(s)	735, 1740, 2638, 2851
MvaI	CCWGG	4 cut(s)	1746, 2069, 3034, 3133
MvnI	CGCG	3 cut(s)	344, 407, 502
MwoI	GCNNNNNNNGC	7 cut(s)	271, 499, 659, 880, 1814, 2848, 3187
NarI	GGCGCC	1 cut(s)	3285
NciI	CCSGG	2 cut(s)	69, 799
NdeI	CATATG	2 cut(s)	633, 1435
NmeAIII	GCCGAG	1 cut(s)	255
NmuCI	GTSAC	8 cut(s)	1322, 1529, 1689, 2009, 2363, 3290, 3441, 3492
NsiI	ATGCAT	2 cut(s)	452, 1671
NspI	RCATGY	5 cut(s)	1298, 1490, 2035, 3278, 3406
NspV	TTCGAA	1 cut(s)	195
PaeI	GCATGC	1 cut(s)	1298
PaeR7I	CTCGAG	1 cut(s)	1626
PagI	TCATGA	2 cut(s)	571, 3240
PaqCI	CACCTGC	1 cut(s)	2507
PciI	ACATGT	1 cut(s)	1486
PciSI	GCTCTTC	1 cut(s)	978
PcsI	WCGNNNNNNNCGW	2 cut(s)	201, 2239
PctI	GAATGC	4 cut(s)	735, 1740, 2638, 2851
PdmI	GAANNNNTTC	3 cut(s)	2229, 2616, 2892
PfeI	GAWTC	8 cut(s)	151, 321, 362, 425, 1178, 1598, 1716, 2180
Pfl23II	CGTACG	1 cut(s)	3069
PflFI	GACNNNGTC	1 cut(s)	298
PfoI	TCCNGGA	1 cut(s)	2067
PleI	GAGTC	4 cut(s)	91, 289, 2827, 3499
PluTI	GGCGCC	1 cut(s)	3288
PpsI	GAGTC	4 cut(s)	91, 289, 2827, 3499
Ppu21I	YACGTR	1 cut(s)	2243
PpuMI	RGGWCCY	1 cut(s)	2865
PscI	ACATGT	1 cut(s)	1486
PshAI	GACNNNNGTC	1 cut(s)	873
Psp124BI	GAGCTC	1 cut(s)	579
Psp1406I	AACGTT	2 cut(s)	2649, 2733
Psp5II	RGGWCCY	1 cut(s)	2865
Psp6I	CCWGG	4 cut(s)	1744, 2067, 3032, 3131
PspGI	CCWGG	4 cut(s)	1744, 2067, 3032, 3131
PspLI	CGTACG	1 cut(s)	3069
PspOMI	GGGCCC	1 cut(s)	894
PspPI	GGNCC	8 cut(s)	894, 895, 1417, 2458, 2476, 2593, 2768, 2865
PspPPI	RGGWCCY	1 cut(s)	2865
PspXI	VCTCGAGB	1 cut(s)	1626
PstI	CTGCAG	1 cut(s)	2133
PsuI	RGATCY	3 cut(s)	135, 1774, 2545
PsyI	GACNNNGTC	1 cut(s)	298
PvuII	CAGCTG	1 cut(s)	3448
RseI	CAYNNNNRTG	2 cut(s)	2151, 3279
SacI	GAGCTC	1 cut(s)	579
SapI	GCTCTTC	1 cut(s)	978
SaqAI	TTAA	8 cut(s)	132, 558, 677, 1544, 2534, 2645, 3168, 3279
Sau96I	GGNCC	8 cut(s)	894, 895, 1417, 2458, 2476, 2593, 2768, 2865
ScaI	AGTACT	2 cut(s)	1348, 2531
SchI	GAGTC	4 cut(s)	91, 289, 2828, 3500
ScrFI	CCNGG	6 cut(s)	69, 799, 1746, 2069, 3034, 3133
SduI	GDGCHC	9 cut(s)	11, 579, 898, 990, 1390, 2727, 2839, 3062, 3391
SfaNI	GCATC	7 cut(s)	459, 1630, 1656, 1795, 2074, 3208, 3490
SfcI	CTRYAG	4 cut(s)	1063, 1192, 1327, 2129
SfoI	GGCGCC	1 cut(s)	3286
Sfr274I	CTCGAG	1 cut(s)	1626
SfuI	TTCGAA	1 cut(s)	195
SinI	GGWCC	5 cut(s)	1417, 2458, 2476, 2768, 2865
SlaI	CTCGAG	1 cut(s)	1626
SmiMI	CAYNNNNRTG	2 cut(s)	2151, 3279
SmlI	CTYRAG	5 cut(s)	131, 656, 1562, 1626, 1943
SmoI	CTYRAG	5 cut(s)	131, 656, 1562, 1626, 1943
SphI	GCATGC	1 cut(s)	1298
SspDI	GGCGCC	1 cut(s)	3284
SspMI	CTAG	3 cut(s)	165, 2570, 3186
SstI	GAGCTC	1 cut(s)	579
StyD4I	CCNGG	6 cut(s)	67, 797, 1744, 2067, 3032, 3131
StyI	CCWWGG	2 cut(s)	164, 3486
TaaI	ACNGT	6 cut(s)	385, 750, 1331, 1483, 1549, 3397
TaiI	ACGT	6 cut(s)	80, 1767, 1813, 2245, 2652, 2736
TaqII	GACCGA	1 cut(s)	288
TatI	WGTACW	8 cut(s)	378, 941, 1346, 1483, 2028, 2143, 2529, 3408
TauI	GCSGC	4 cut(s)	37, 316, 344, 502
TfiI	GAWTC	8 cut(s)	151, 321, 362, 425, 1178, 1598, 1716, 2180
Tru1I	TTAA	8 cut(s)	132, 558, 677, 1544, 2534, 2645, 3168, 3279
Tru9I	TTAA	8 cut(s)	132, 558, 677, 1544, 2534, 2645, 3168, 3279
TseFI	GTSAC	8 cut(s)	1322, 1529, 1689, 2009, 2363, 3290, 3441, 3492
TseI	GCWGC	8 cut(s)	1049, 1188, 1226, 1643, 2128, 2574, 2748, 2956
Tsp45I	GTSAC	8 cut(s)	1322, 1529, 1689, 2009, 2363, 3290, 3441, 3492
TspGWI	ACGGA	7 cut(s)	73, 297, 322, 1349, 2396, 2519, 3505
Tth111I	GACNNNGTC	1 cut(s)	298
Vha464I	CTTAAG	1 cut(s)	131
VneI	GTGCAC	1 cut(s)	1386
VpaK11BI	GGWCC	5 cut(s)	1417, 2458, 2476, 2768, 2865
XagI	CCTNNNNNAGG	1 cut(s)	1830
XapI	RAATTY	7 cut(s)	191, 349, 827, 1038, 1339, 3000, 3436
XceI	RCATGY	5 cut(s)	1298, 1490, 2035, 3278, 3406
XcmI	CCANNNNNNNNNTGG	1 cut(s)	968
XhoI	CTCGAG	1 cut(s)	1626
XmaJI	CCTAGG	1 cut(s)	164
XmiI	GTMKAC	2 cut(s)	743, 2239
XmnI	GAANNNNTTC	3 cut(s)	2229, 2616, 2892
XspI	CTAG	3 cut(s)	165, 2570, 3186
ZraI	GACGTC	1 cut(s)	78
ZrmI	AGTACT	2 cut(s)	1348, 2531
Zsp2I	ATGCAT	2 cut(s)	452, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.