RchiOBHm_Chr1g0374881

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008055
Physical Location & Seq
Reverse (-)
62531343 .. 62538219
6877 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59864

Sequence Viewer

Length: 3525 bp
ATGTCGAGCGATCCGAATCCAAACCCACCGCCTCTGACCTCGACCTCCGCCGGCGGCAACGCAGACCCCGCCGCCGCATCGTCTCCGACTCATCCTTCTCCCTCCAGAGCCAAGCTCCGTAAGATTCCTCCCATTCCGATTCGCCGGAGCCCCAGGCATTTGGCCGAGGTCAACGAACAAAGCGACGAAGAGGGAGACGATGATGAAGATGACGACGACGATGATTTTAGGGAACGCAGAGAGACCACGCCGATCCTATTGGCTGCGTCGCTAGGGCTCAATCACATACGGACTCGATCGGCGCCGTCGCCGCTCCGGTTCTCTTCCTCCGCCGGCGAGCCTCTAATTCCCGGTGAGGAAGCGAATAATATGAACTACACGGTCAAGTCAAAGCCCCAACCAGTGAAATATGTGCACTCGAGTCAATCAAAATCGCTGAGAACTCCATCGCCACTCAATCCTGTGTTGGAGGGTGGTCAAGCTGGTCTTACAAAGGAAATGCAGTCGCCTAGGTTTCAGGCAATACTGCGAGTTACGAGTGGACGAAAGAAGAGAACACCTGACATCAAGAGTTTCTCTCATGAGCTAAATTCCAAAGGGGTTAGACCATTTCCGGTGTGGAAGTCCCGTGCATTCGGGCATATGGAGGAGGTTTTGGTGGCGCTCAGGGCAAGATTTGAGAGGTTGAAGGATGAGGTTGATGTTGATTTGGGTGCATTTGCGGGTGATTTGGTGGGTATTCTTGAAAAGTCTTCAGAGTCTCACCCTGAATGGAAAGAGAATTTTGAGGATTTGCTAGTTATTGCCCGGCAGTGTGCAAAGATGTCTCCTAACGAATTTTGGATCAAGTGTGAAGGCATTGTACAGAAGTTGGATGACCGGCGTCAAGAGCTACCAATGGGGGCCCTGAAACAAGCCCATACTCGTCTTCTTTTTATTCTTACTCGGTGCACACGGCTTGTGCAGTTCCACAAAGAGAGTGGTTATGAGGAAGAGCACATTCTTAGTCTTCACCAGCTCAGTGACCTTGGTGTTTATCCAGAACAAATTTTGGACGGTGCAGAACAGAAGTTTAGTGGTCCACTGAGTGGGAAAGATGCAAACGAGAAGCCCAAGAAGTCTCGTGAGCAAGAAATAGCTAGTGGAGCTGATGGTGTGGACGTTAGCACTGCCAAGAGCGTTGAATCTACTGGTAGCTATAGGATGTCATCTTGGAAAAAACTTCCATCGGCTGCTGAGAAAAAGCAGAAAGTACCTGATTCTGTTGATACACCATCGAAGGACAAATCAGAACGTTTGCATGCCAAAGAAAATCTGGATACTCCATCATCCCATCATGAACACTCAGAGATGTCCACAAGTGCACAAAGAGTATCTTGGGTTTCAATTGGGGAGCAGCAAAATGTTACATATGAGAATTTGATGATCTGTCGTATATGTGAGGTTGAAATACCAACTGTCCACGTAGAGGAGCACTCTCGAATATGTACAATTGCTGACAGATGTGACTTGAAGGGCTTAACTGTGAATGAAAGACTTGAAAGAGTTGCTGAAGCTCTTGAAAGGATAATGGAATCTCGGACAACAAAAAATGTTTATACACAAGAGACAAGTTTTGATGCTGCAAAAGTGTATCCATCAAGTATCCATGAAGATTTTATTGCCAAAAGAAAAAAAAGTATCCATGAAGATTTAGATGACTTATCACCTAAACAAAATGACTTGCCTCAAGACATGGTTGATTGTGTTCCTGATGCTGACAATTCTTTTGTCATGGAAGATCTTAATATTATGCCTGACATGTCATGTGACGCACGTTCTGCTTTGACACCTGAACAAGGGACCAGAACCTCATCAGCAGGTAGCTCCACTCCAAGATCGCCACTTTTAACCCCTAGAACCACTCATATTGAAATGCTGTTGAGTGGAAGGGGAACAATACCAGAACTTGAGAATTGTCAGCAGATACACAAGCTACTGGATATTGCTCGTTCTGTTGCAAATGTTAGCAATTGTGAATACAGTGCATTGGAGTATATGCTTGACCGACTAGAAGACCTAAAATATGCTATCCAGGACAGGAAGGTGGATGCCCTTGTTGTAGAAACGTTTGGAAGACGTATTGAAAAATTATTGCAGGAAAAATATGTACATCTTTGCGGGCAGATAGAAGATGAGAAATTGGATTCATCAAATGGTATGGCAGACGAAGAAAGTTCAGTGGAAGATGATACAGTTCGTAGTCTACGTACCAGCCCCATCAATCCGTCTTCTAAGGATCGAACATCTATTGAAGATTTTGAAATAATAAAACCAATAAGCAGGGGTGCATTTGGACGAGTTTTTCTTGCCAGAAAACGAGCGACTGGTGACTTATTTGCTATAAAGGTATTAAAGAAGGCTGATATGATTCGTAAGAATGCAGTTGAAAGTATTTTGGCAGAGCGTAACATCCTTATATTGGTTCGCAATCCTTTTGTGGTCCGATTTTTCTATTCCTTCACATGCAGGGAAAATCTTTATCTGGTCATGGAGTACTTAAATGGTGGAGATCTTTACTCTTTATTGAGAAATCTAGGCTGCTTAGATGAAGATATGGCCCGTGTATATATTGCAGAAGTTGTTCTTGCTTTGGAGTATCTGCATTCATTAAATGTTATTCATAGAGACTTGAAGCCAGACAACTTGTTGATTGGTCAAGATGGTCACATCAAGTTGACAGATTTTGGGCTCTCCAAGGTTGGTCTAATCAGCAGCACTGATGACTTAGCAGGTCCATCTGTTGGTGATACTGGTTTTGTTGGAGATGATGAACCAAAAAGTCAATCTTCATCTCAGAGGTCACAGCGTCAAAAACATTCAGTTGTTGGCACCCCTGATTATTTGGCACCTGAAATACTTCTTGGCATGGGACATAGTGCAACTGCTGATTGGTGGTCTGTGGGTGTTATACTTTTTGAGCTACTTGTTGGCATTCCACCATTCAATGCAGAACATCCACAGCAAATTTTCAACAATATAATAAACAGAGATATCCCCTGGCCTAAGGTACCTGAGGAGATGAGCAACGAAGCATATGACTTAATTGACCAATTACTGACTGAAAACCCAGTTCAAAGACTTGGAGCAACAGGTGCTAGGGAGGTGAAGCAACACACTTTTTTCAAGGATATTAACTGGGATACACTTGCAAGGCAGAAGGCTATGTTCATACCTTCAACAGATGCACATGATACAAGTTATTTCATGAGCCGGTACATATGGAATCCAGAAGATGAGCATGTTAATGGAGGCAGCGACTTTGATGACCTGACTGAAACATGCAGCAGTACTGGTTCATATTATCTAGATGAAGACGGGGATGAGTGTGGTAGTTTGGCGGACTTCAGTGCTCCAGCCCTTGATGTGCAGTACTCATTTAGTAATTTCTCATTTAAGAACTTGTCTCAGCTGGCTTCCATCAATTATGATCTTGTCGTGAAGACCAAGGAGTCACCAGAAGCCTCCAAGTCATCTGTTCCATGA

Protein Analysis

1174

Amino Acids

131.27

Weight (kDa)

5.22

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IREH1 PF26031 207 - 325 9e-56 IREH1-like domain
Pkinase PF00069 767 - 1055 4.9e-65 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 768 - 914 1.1e-24 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 946 - 1041 4.4e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3490
Acc36I ACCTGC 2 cut(s) 1850, 2762
Acc65I GGTACC 1 cut(s) 3049
AccB1I GGYRCC 4 cut(s) 301, 2870, 2887, 3049
AccB7I CCANNNNNTGG 2 cut(s) 1088, 2783
AccBSI CCGCTC 1 cut(s) 313
AccI GTMKAC 1 cut(s) 2245
AclI AACGTT 2 cut(s) 1294, 2108
AclWI GGATC 4 cut(s) 5, 247, 851, 2286
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 6 cut(s) 589, 781, 836, 1047, 1417, 3006
AcuI CTGAAG 3 cut(s) 738, 1572, 3370
AcyI GRCGYC 2 cut(s) 302, 883
AdeI CACNNNGTG 1 cut(s) 1088
AfiI CCNNNNNNNGG 5 cut(s) 1088, 1468, 1838, 2461, 2783
AflIII ACRYGT 1 cut(s) 1800
AjnI CCWGG 3 cut(s) 152, 2073, 3038
AjuI GAANNNNNNNTTGG 2 cut(s) 2886, 2918
Alw21I GWGCWC 6 cut(s) 417, 953, 999, 1366, 1476, 3394
Alw26I GTCTC 9 cut(s) 87, 189, 236, 765, 831, 1125, 1601, 2661, 3450
Alw44I GTGCAC 3 cut(s) 413, 949, 1362
AlwI GGATC 4 cut(s) 5, 247, 851, 2286
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 4 cut(s) 162, 903, 2598, 3041
ApaI GGGCCC 1 cut(s) 907
ApaLI GTGCAC 3 cut(s) 413, 949, 1362
ApeKI GCWGC 8 cut(s) 263, 1232, 1396, 1622, 2580, 2754, 3294, 3324
ApoI RAATTY 6 cut(s) 589, 781, 836, 1047, 1417, 3006
ArsI GACNNNNNNTTYG 2 cut(s) 1751, 1783
Asp700I GAANNNNTTC 2 cut(s) 2622, 2898
Asp718I GGTACC 1 cut(s) 3049
AspA2I CCTAGG 1 cut(s) 509
AspLEI GCGC 2 cut(s) 304, 664
AspS9I GGNCC 7 cut(s) 903, 904, 1079, 1842, 2482, 2599, 2774
AsuC2I CCSGG 2 cut(s) 351, 808
AsuHPI GGTGA 9 cut(s) 365, 737, 755, 1004, 1698, 2381, 2798, 3157, 3486
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 4 cut(s) 1079, 1842, 2482, 2774
AvrII CCTAGG 1 cut(s) 509
AxyI CCTNAGG 2 cut(s) 3045, 3054
BaeGI GKGCMC 4 cut(s) 417, 907, 953, 1366
BanI GGYRCC 4 cut(s) 301, 2870, 2887, 3049
BanII GRGCYC 4 cut(s) 152, 279, 907, 2733
BarI GAAGNNNNNNTAC 2 cut(s) 846, 878
BauI CACGAG 1 cut(s) 1122
BbsI GAAGAC 8 cut(s) 744, 920, 1001, 2061, 2122, 2262, 3360, 3488
Bbv12I GWGCWC 6 cut(s) 417, 953, 999, 1366, 1476, 3394
BbvI GCAGC 8 cut(s) 250, 1219, 1408, 1609, 2567, 2766, 3306, 3336
BceAI ACGGC 2 cut(s) 289, 971
BciT130I CCWGG 3 cut(s) 154, 2075, 3040
BciVI GTATCC 5 cut(s) 1312, 1644, 1655, 1691, 3175
BcnI CCSGG 2 cut(s) 351, 808
BcoDI GTCTC 9 cut(s) 87, 189, 236, 765, 831, 1125, 1601, 2661, 3450
BfaI CTAG 9 cut(s) 272, 510, 797, 1140, 1896, 2051, 2576, 3138, 3347
BfmI CTRYAG 1 cut(s) 1198
BfoI RGCGCY 2 cut(s) 305, 665
BfuAI ACCTGC 2 cut(s) 1850, 2762
BfuI GTATCC 5 cut(s) 1312, 1644, 1655, 1691, 3175
BglII AGATCT 2 cut(s) 1780, 2551
BlnI CCTAGG 1 cut(s) 509
BmcAI AGTACT 3 cut(s) 2537, 3331, 3413
Bme1390I CCNGG 5 cut(s) 154, 351, 808, 2075, 3040
Bme18I GGWCC 4 cut(s) 1079, 1842, 2482, 2774
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 7 cut(s) 903, 904, 1079, 1842, 2482, 2599, 2774
BmiI GGNNCC 8 cut(s) 149, 303, 904, 905, 1843, 2872, 2889, 3051
BmrFI CCNGG 5 cut(s) 154, 351, 808, 2075, 3040
BmrI ACTGGG 2 cut(s) 3104, 3187
BmsI GCATC 6 cut(s) 86, 1087, 1609, 1744, 2080, 3214
BmuI ACTGGG 2 cut(s) 3104, 3187
BoxI GACNNNNGTC 1 cut(s) 882
BpiI GAAGAC 8 cut(s) 744, 920, 1001, 2061, 2122, 2262, 3360, 3488
BplI GAGNNNNNCTC 6 cut(s) 99, 131, 562, 594, 1460, 1492
BpmI CTGGAG 2 cut(s) 88, 3378
Bpu10I CCTNAGC 1 cut(s) 665
BpuEI CTTGAG 2 cut(s) 1713, 1970
BpuMI CCSGG 2 cut(s) 351, 808
BsaAI YACGTR 2 cut(s) 1465, 2249
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 2 cut(s) 302, 883
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 7 cut(s) 152, 165, 509, 1027, 2736, 3038, 3486
BsaWI WCCGGW 2 cut(s) 315, 613
Bsc4I CCNNNNNNNGG 5 cut(s) 1088, 1468, 1838, 2461, 2783
Bse118I RCCGGY 4 cut(s) 50, 332, 879, 3252
Bse1I ACTGG 8 cut(s) 401, 1195, 1983, 2371, 2797, 3110, 3182, 3337
Bse21I CCTNAGG 2 cut(s) 3045, 3054
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 3 cut(s) 154, 2075, 3040
BseDI CCNNGG 7 cut(s) 152, 165, 509, 1027, 2736, 3038, 3486
BseGI GGATG 9 cut(s) 91, 697, 880, 1209, 1328, 2095, 2451, 2995, 3367
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 5 cut(s) 1088, 1468, 1838, 2461, 2783
BseMII CTCAG 9 cut(s) 428, 679, 1033, 1076, 1227, 1359, 2849, 3045, 3461
BseNI ACTGG 8 cut(s) 401, 1195, 1983, 2371, 2797, 3110, 3182, 3337
BseRI GAGGAG 3 cut(s) 662, 1484, 3071
BseSI GKGCMC 4 cut(s) 417, 907, 953, 1366
BseXI GCAGC 8 cut(s) 250, 1219, 1408, 1609, 2567, 2766, 3306, 3336
BsgI GTGCAG 3 cut(s) 983, 1080, 3428
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 4 cut(s) 164, 905, 2600, 3043
BshNI GGYRCC 4 cut(s) 301, 2870, 2887, 3049
BsiEI CGRYCG 1 cut(s) 299
BsiHKAI GWGCWC 6 cut(s) 417, 953, 999, 1366, 1476, 3394
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 9 cut(s) 51, 145, 316, 333, 351, 614, 808, 880, 3253
BslFI GGGAC 3 cut(s) 610, 1855, 2925
BslI CCNNNNNNNGG 5 cut(s) 1088, 1468, 1838, 2461, 2783
BsmAI GTCTC 9 cut(s) 87, 189, 236, 765, 831, 1125, 1601, 2661, 3450
BsmBI CGTCTC 2 cut(s) 87, 189
BsmFI GGGAC 3 cut(s) 610, 1855, 2925
BsmI GAATGC 4 cut(s) 632, 2425, 2644, 2973
BsnI GGCC 4 cut(s) 164, 905, 2600, 3043
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 418
Bsp120I GGGCCC 1 cut(s) 903
Bsp1407I TGTACA 3 cut(s) 862, 1487, 2149
BspANI GGCC 4 cut(s) 164, 905, 2600, 3043
BspCNI CTCAG 9 cut(s) 429, 678, 1032, 1077, 1228, 1358, 2848, 3046, 3460
BspHI TCATGA 3 cut(s) 580, 1336, 3246
BspLI GGNNCC 8 cut(s) 149, 303, 904, 905, 1843, 2872, 2889, 3051
BspMI ACCTGC 2 cut(s) 1850, 2762
BspPI GGATC 4 cut(s) 5, 247, 851, 2286
BspQI GCTCTTC 1 cut(s) 987
BspT107I GGYRCC 4 cut(s) 301, 2870, 2887, 3049
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 313
BsrFI RCCGGY 4 cut(s) 50, 332, 879, 3252
BsrGI TGTACA 3 cut(s) 862, 1487, 2149
BsrI ACTGG 8 cut(s) 401, 1195, 1983, 2371, 2797, 3110, 3182, 3337
BssAI RCCGGY 4 cut(s) 50, 332, 879, 3252
BssECI CCNNGG 7 cut(s) 152, 165, 509, 1027, 2736, 3038, 3486
BssNI GRCGYC 2 cut(s) 302, 883
BssSI CACGAG 1 cut(s) 1122
BssT1I CCWWGG 4 cut(s) 509, 1027, 2736, 3486
Bst2BI CACGAG 1 cut(s) 1122
Bst2UI CCWGG 3 cut(s) 154, 2075, 3040
Bst4CI ACNGT 6 cut(s) 382, 1058, 1459, 1525, 2024, 2236
Bst6I CTCTTC 4 cut(s) 183, 328, 545, 987
BstACI GRCGYC 2 cut(s) 302, 883
BstAPI GCANNNNNTGC 2 cut(s) 1820, 3134
BstAUI TGTACA 3 cut(s) 862, 1487, 2149
BstBAI YACGTR 2 cut(s) 1465, 2249
BstC8I GCNNGC 6 cut(s) 52, 334, 338, 1302, 2162, 3453
BstENI CCTNNNNNAGG 1 cut(s) 1836
BstF5I GGATG 9 cut(s) 91, 697, 880, 1209, 1328, 2095, 2451, 2995, 3367
BstH2I RGCGCY 2 cut(s) 305, 665
BstHHI GCGC 2 cut(s) 304, 664
BstMAI GTCTC 9 cut(s) 87, 189, 236, 765, 831, 1125, 1601, 2661, 3450
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 7 cut(s) 68, 310, 668, 889, 1145, 1820, 3134
BstNI CCWGG 3 cut(s) 154, 2075, 3040
BstNSI RCATGY 5 cut(s) 1304, 1804, 2508, 3284, 3324
BstPAI GACNNNNGTC 1 cut(s) 882
BstSCI CCNGG 5 cut(s) 152, 349, 806, 2073, 3038
BstSFI CTRYAG 1 cut(s) 1198
BstSLI GKGCMC 4 cut(s) 417, 907, 953, 1366
BstSNI TACGTA 1 cut(s) 2249
BstV1I GCAGC 8 cut(s) 250, 1219, 1408, 1609, 2567, 2766, 3306, 3336
BstV2I GAAGAC 8 cut(s) 744, 920, 1001, 2061, 2122, 2262, 3360, 3488
BstX2I RGATCY 2 cut(s) 1780, 2551
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 2 cut(s) 1780, 2551
Bsu36I CCTNAGG 2 cut(s) 3045, 3054
BsuI GTATCC 5 cut(s) 1312, 1644, 1655, 1691, 3175
BsuRI GGCC 4 cut(s) 164, 905, 2600, 3043
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 9 cut(s) 91, 697, 880, 1209, 1328, 2095, 2451, 2995, 3367
BtsI GCAGTG 2 cut(s) 818, 1167
BtsIMutI CAGTG 9 cut(s) 408, 818, 1027, 1082, 1167, 2029, 2226, 2757, 3394
BveI ACCTGC 2 cut(s) 1850, 2762
Cac8I GCNNGC 6 cut(s) 52, 334, 338, 1302, 2162, 3453
CciI TCATGA 3 cut(s) 580, 1336, 3246
CfoI GCGC 2 cut(s) 304, 664
Cfr10I RCCGGY 4 cut(s) 50, 332, 879, 3252
Cfr13I GGNCC 7 cut(s) 903, 904, 1079, 1842, 2482, 2599, 2774
CseI GACGC 4 cut(s) 255, 872, 1820, 2837
CspCI CAANNNNNGTGG 4 cut(s) 961, 996, 1891, 1926
DinI GGCGCC 1 cut(s) 303
DraIII CACNNNGTG 1 cut(s) 1088
DrdI GACNNNNNNGTC 1 cut(s) 3490
DseDI GACNNNNNNGTC 1 cut(s) 3490
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 4 cut(s) 183, 328, 545, 987
EarI CTCTTC 4 cut(s) 183, 328, 545, 987
EciI GGCGGA 3 cut(s) 37, 319, 3395
Eco105I TACGTA 1 cut(s) 2249
Eco130I CCWWGG 4 cut(s) 509, 1027, 2736, 3486
Eco24I GRGCYC 4 cut(s) 152, 279, 907, 2733
Eco31I GGTCTC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 3034
Eco47I GGWCC 4 cut(s) 1079, 1842, 2482, 2774
Eco57I CTGAAG 3 cut(s) 738, 1572, 3370
Eco81I CCTNAGG 2 cut(s) 3045, 3054
Eco88I CYCGRG 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 1836
EcoO109I RGGNCCY 2 cut(s) 903, 904
EcoRII CCWGG 3 cut(s) 152, 2073, 3038
EcoRV GATATC 1 cut(s) 3034
EcoT14I CCWWGG 4 cut(s) 509, 1027, 2736, 3486
EcoT38I GRGCYC 4 cut(s) 152, 279, 907, 2733
EgeI GGCGCC 1 cut(s) 303
EheI GGCGCC 1 cut(s) 303
ErhI CCWWGG 4 cut(s) 509, 1027, 2736, 3486
Esp3I CGTCTC 2 cut(s) 87, 189
FalI AAGNNNNNCTT 8 cut(s) 471, 503, 1360, 1392, 2610, 2642, 2812, 2844
FaqI GGGAC 3 cut(s) 610, 1855, 2925
FauI CCCGC 3 cut(s) 76, 715, 2153
FauNDI CATATG 4 cut(s) 642, 1411, 3076, 3260
FblI GTMKAC 1 cut(s) 2245
FokI GGATG 9 cut(s) 78, 704, 887, 1216, 1315, 2102, 2438, 2982, 3374
FriOI GRGCYC 4 cut(s) 152, 279, 907, 2733
FspBI CTAG 9 cut(s) 272, 510, 797, 1140, 1896, 2051, 2576, 3138, 3347
GlaI GCGC 2 cut(s) 303, 663
GsuI CTGGAG 2 cut(s) 88, 3378
HaeII RGCGCY 2 cut(s) 305, 665
HaeIII GGCC 4 cut(s) 164, 905, 2600, 3043
HapII CCGG 9 cut(s) 51, 145, 316, 333, 351, 614, 808, 880, 3253
HgaI GACGC 4 cut(s) 255, 872, 1820, 2837
HhaI GCGC 2 cut(s) 304, 664
Hin1I GRCGYC 2 cut(s) 302, 883
Hin6I GCGC 2 cut(s) 302, 662
HinP1I GCGC 2 cut(s) 302, 662
HincII GTYRAC 2 cut(s) 172, 2718
HindII GTYRAC 2 cut(s) 172, 2718
HpaII CCGG 9 cut(s) 51, 145, 316, 333, 351, 614, 808, 880, 3253
HphI GGTGA 9 cut(s) 365, 737, 755, 1004, 1698, 2381, 2798, 3157, 3486
Hpy99I CGWCG 5 cut(s) 188, 218, 221, 271, 310
HpyCH4III ACNGT 6 cut(s) 382, 1058, 1459, 1525, 2024, 2236
HpyCH4IV ACGT 7 cut(s) 1161, 1294, 1464, 1816, 2108, 2119, 2248
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 310, 668, 889, 1145, 1820, 3134
HpySE526I ACGT 7 cut(s) 1161, 1294, 1464, 1816, 2108, 2119, 2248
Hsp92I GRCGYC 2 cut(s) 302, 883
HspAI GCGC 2 cut(s) 302, 662
KasI GGCGCC 1 cut(s) 301
KpnI GGTACC 1 cut(s) 3053
KroI GCCGGC 2 cut(s) 50, 332
KroNI GCCGGC 2 cut(s) 52, 334
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 9 cut(s) 120, 147, 318, 1145, 1393, 1471, 1871, 3125, 3397
Lsp1109I GCAGC 8 cut(s) 250, 1219, 1408, 1609, 2567, 2766, 3306, 3336
LweI GCATC 6 cut(s) 86, 1087, 1609, 1744, 2080, 3214
MaeI CTAG 9 cut(s) 272, 510, 797, 1140, 1896, 2051, 2576, 3138, 3347
MaeII ACGT 7 cut(s) 1161, 1294, 1464, 1816, 2108, 2119, 2248
MbiI CCGCTC 1 cut(s) 313
MfeI CAATTG 3 cut(s) 1386, 1491, 2011
MflI RGATCY 2 cut(s) 1780, 2551
Mly113I GGCGCC 1 cut(s) 302
MlyI GAGTC 5 cut(s) 82, 286, 430, 767, 3500
MmeI TCCRAC 4 cut(s) 110, 447, 852, 2782
MreI CGCCGGCG 2 cut(s) 50, 332
MroNI GCCGGC 2 cut(s) 50, 332
MroXI GAANNNNTTC 2 cut(s) 2622, 2898
MslI CAYNNNNRTG 1 cut(s) 3285
MspA1I CMGCKG 1 cut(s) 3451
MspI CCGG 9 cut(s) 51, 145, 316, 333, 351, 614, 808, 880, 3253
MspR9I CCNGG 5 cut(s) 154, 351, 808, 2075, 3040
MunI CAATTG 3 cut(s) 1386, 1491, 2011
Mva1269I GAATGC 4 cut(s) 632, 2425, 2644, 2973
MvaI CCWGG 3 cut(s) 154, 2075, 3040
MwoI GCNNNNNNNGC 7 cut(s) 68, 310, 668, 889, 1145, 1820, 3134
NaeI GCCGGC 2 cut(s) 52, 334
NarI GGCGCC 1 cut(s) 302
NciI CCSGG 2 cut(s) 351, 808
NdeI CATATG 4 cut(s) 642, 1411, 3076, 3260
NgoMIV GCCGGC 2 cut(s) 50, 332
NlaIV GGNNCC 8 cut(s) 149, 303, 904, 905, 1843, 2872, 2889, 3051
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 7 cut(s) 1022, 1505, 1808, 2369, 2705, 2841, 3492
NspI RCATGY 5 cut(s) 1304, 1804, 2508, 3284, 3324
PaeI GCATGC 1 cut(s) 1304
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 3 cut(s) 580, 1336, 3246
PciI ACATGT 1 cut(s) 1800
PciSI GCTCTTC 1 cut(s) 987
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 180, 2245
PctI GAATGC 4 cut(s) 632, 2425, 2644, 2973
PdiI GCCGGC 2 cut(s) 52, 334
PdmI GAANNNNTTC 2 cut(s) 2622, 2898
PfeI GAWTC 9 cut(s) 16, 124, 139, 1184, 1259, 1574, 2186, 2410, 3265
PflMI CCANNNNNTGG 2 cut(s) 1088, 2783
PfoI TCCNGGA 1 cut(s) 2073
Ple19I CGATCG 1 cut(s) 299
PleI GAGTC 5 cut(s) 82, 286, 429, 766, 3499
PluTI GGCGCC 1 cut(s) 305
PpsI GAGTC 5 cut(s) 82, 286, 429, 766, 3499
Ppu21I YACGTR 2 cut(s) 1465, 2249
PscI ACATGT 1 cut(s) 1800
PshAI GACNNNNGTC 1 cut(s) 882
Psp1406I AACGTT 2 cut(s) 1294, 2108
Psp6I CCWGG 3 cut(s) 152, 2073, 3038
PspGI CCWGG 3 cut(s) 152, 2073, 3038
PspN4I GGNNCC 8 cut(s) 149, 303, 904, 905, 1843, 2872, 2889, 3051
PspOMI GGGCCC 1 cut(s) 903
PspPI GGNCC 7 cut(s) 903, 904, 1079, 1842, 2482, 2599, 2774
PspXI VCTCGAGB 1 cut(s) 418
PsuI RGATCY 2 cut(s) 1780, 2551
PvuI CGATCG 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 3451
RseI CAYNNNNRTG 1 cut(s) 3285
SapI GCTCTTC 1 cut(s) 987
Sau96I GGNCC 7 cut(s) 903, 904, 1079, 1842, 2482, 2599, 2774
ScaI AGTACT 3 cut(s) 2537, 3331, 3413
SchI GAGTC 5 cut(s) 82, 286, 430, 767, 3500
ScrFI CCNGG 5 cut(s) 154, 351, 808, 2075, 3040
SfaNI GCATC 6 cut(s) 86, 1087, 1609, 1744, 2080, 3214
SfcI CTRYAG 1 cut(s) 1198
SfoI GGCGCC 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 418
SgrAI CRCCGGYG 2 cut(s) 50, 332
SinI GGWCC 4 cut(s) 1079, 1842, 2482, 2774
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 3285
SmlI CTYRAG 3 cut(s) 418, 1728, 1949
SmoI CTYRAG 3 cut(s) 418, 1728, 1949
SnaBI TACGTA 1 cut(s) 2249
SphI GCATGC 1 cut(s) 1304
SspDI GGCGCC 1 cut(s) 301
SspI AATATT 1 cut(s) 1789
SspMI CTAG 9 cut(s) 272, 510, 797, 1140, 1896, 2051, 2576, 3138, 3347
StyD4I CCNGG 5 cut(s) 152, 349, 806, 2073, 3038
StyI CCWWGG 4 cut(s) 509, 1027, 2736, 3486
TaaI ACNGT 6 cut(s) 382, 1058, 1459, 1525, 2024, 2236
TaiI ACGT 7 cut(s) 1164, 1297, 1467, 1819, 2111, 2122, 2251
TaqI TCGA 7 cut(s) 5, 41, 295, 419, 1277, 1480, 2281
TaqII GACCGA 1 cut(s) 2061
TatI WGTACW 6 cut(s) 862, 1487, 2149, 2535, 3329, 3411
TauI GCSGC 4 cut(s) 57, 74, 77, 313
TfiI GAWTC 9 cut(s) 16, 124, 139, 1184, 1259, 1574, 2186, 2410, 3265
TscAI CASTG 9 cut(s) 408, 818, 1027, 1089, 1174, 2029, 2226, 2764, 3394
TseFI GTSAC 7 cut(s) 1022, 1505, 1808, 2369, 2705, 2841, 3492
TseI GCWGC 8 cut(s) 263, 1232, 1396, 1622, 2580, 2754, 3294, 3324
Tsp45I GTSAC 7 cut(s) 1022, 1505, 1808, 2369, 2705, 2841, 3492
TspGWI ACGGA 3 cut(s) 107, 304, 2256
TspRI CASTG 9 cut(s) 408, 818, 1027, 1089, 1174, 2029, 2226, 2764, 3394
Van91I CCANNNNNTGG 2 cut(s) 1088, 2783
VneI GTGCAC 3 cut(s) 413, 949, 1362
VpaK11BI GGWCC 4 cut(s) 1079, 1842, 2482, 2774
XagI CCTNNNNNAGG 1 cut(s) 1836
XapI RAATTY 6 cut(s) 589, 781, 836, 1047, 1417, 3006
XbaI TCTAGA 1 cut(s) 3346
XceI RCATGY 5 cut(s) 1304, 1804, 2508, 3284, 3324
XcmI CCANNNNNNNNNTGG 3 cut(s) 615, 977, 1312
XhoI CTCGAG 1 cut(s) 418
XmaJI CCTAGG 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 2245
XmnI GAANNNNTTC 2 cut(s) 2622, 2898
XspI CTAG 9 cut(s) 272, 510, 797, 1140, 1896, 2051, 2576, 3138, 3347
ZrmI AGTACT 3 cut(s) 2537, 3331, 3413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.