Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0008055

Physical Location & Seq

Genomic Coordinates Reverse (-)

49118280 .. 49125179

Gene Structure 6900 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG360900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3531 bp

ATGTCGAGCGATCCGAATCCAAACCCACCGCCTCTGACCTCGACCTCCGCCGCCGGCAACGCAGACCCCGCCGCCGCATCGTCTCCGACTCATCCTTCTCCCTCCAGAGCCAAGCTCCGTAAGATTCCTCCCATTCCGATTCGCCGGAGCCCTAGGCATTTGGCCGAGGTCAACGAACAAAGCGACGAAGAGGGAGACGATGATGAAGATGACGACGACGACTATGATTTTAGGGAGCGTAGAGAGACCACGCCGATCCTATTGGCTGCGTCGCTAGGGCTCAATCACATACGGACTCGATCGGCGCCGTCGCCGCTCCGGTTCTCTTCCTCCGCCGGCGAGCCTCTAATTCCCGGTGAGGAAGCGAATAATATGAACTACACGATCAAGTCAAAGCCCCAACCAGTGAAATATGTGCACTCGAGTCAATCAAAATCGCTGAGAACTCCATCGCCACTCAATCCTGTGTTGGAGGGTGGTCAAGCTGGTCTTACAAAGGAAATGCAGTCGCCTAGGTTTCAGGCAATACTGCGAGTTACGAGTGGACGAAAGAAGAGAACACCTGACATCAAGAGTTTCTCTCATGAACTAAATTCCAAAGGAGTTAGACCATTTCCGGTGTGGAAGTCCCGTGCATTCGGGCATATGGAGGAGGTTTTGGTGGCGCTCAGGGCAAGATTTGAGAGGCTGAAGGATGAGGTTGATGTTGATTTGGGTGCATTTGCGGGTGATTTGGTGGGTATACTTGAAAAGTCTTCAGAGTCTCACCCTGAATGGAAAGAGAATTTTGAGGATTTGCTAGTTATTGCCCGGCAGTGTGCAAAGATGTCTCCTAACGAATTTTGGATCAAGTGTGAAGGCATTGTACAGAAGTTGGATGATCGGCGTCAAGAGCTACCAATGGGGGCCCTGAAACAAGCCCATACTCGTCTTCTTTTTATTCTTACTCGGTGCACACGGCTTGTGCAGTTCCAGAAAGAGAGTGGTTATGAGGAAGAGCACATTCTTAGTCTTCACCAGCTCAGTGACCTTGGTGTTTATCCAGAACAAATTTTGGAGGGTGCAGAACAGAAGTTTAGTGGTACACTGAGTGGGAAAGATGCAAACGAGAAGCCCAAGAAGTCTCGTGAGCAAGAAATAGCTAGTGGAGCTGATGGTGTGGAGGTTAGCACTGCCAAGAGCGTTGAATCTACTGGTAGCTATAGGATGTCATCTTGGAAAAAACTTCCATCGGCTGCTGAGAAAAAGCAGAAAGGACCTGATTCTGTTGATACACCATCGAAGGACAAATCAGAACATTTGCATGCCAAAGAAAATCTGGATACTCCATCTTCCCATCATGAACACTCAGAGATGTCCACAAGTGCACAAAGAGTATCTTGGGTTTCAATTGGGGAGCAGCAAAATGTTACATATGAGAATTTGATGATCTGTCGTATATGTGAGGTTGAAATACCAACTGTACACGTAGAGGAGCACTCTCGAATATGTACAATTGCTGACAGATGTGACTTGAAGGGCTTAACTGTGAATGAAAGACTTGAAAGAGTTGCTGAAGCTCTTGAAAGGATAATGGAATCTCGGACAACAAAAAGTGTTGATACACAAGAGACAAGTTTTGATGCTGCAAAAGTGTATCCATCAAGTATCCATGAAGATTTTATTGCCAAAAGAAAAAAAAGTATCCATGAAGATTTAGATGACTTATCACCTAAACGAAATGACTTGCCTCAAGACATGCTTGATTGTGTTCCTGATGCTGACAATTCTTTTGTCATGGAAGATCTTAATATTATGCCTGACATGTCATGTGACGCACGTTCTGCTTTGACACCTGAACAAGGGACCAGAACCTCATCAGCGGGAAGCTCCACTCCAAGATCGCCACTTTTAACCCCTAGAACCACTCATATTGAAATGCTGTTGAGTGGAAGGGGAACAATACCAGAACTTGAGAATTGTCAGCAGATACACAAGCTACTGGATATTGCTCGTTCTGTTGCAAATGTTAGCAATTGTGACTACAGTACATTGGAGTATATGCTTGACCGACTAGAAGACCTAAAATATGCTATCCAGGACAGGAAGGTGGATGCCCTTGTTGTAGAGACGTTTGGAAGACGTATTGAAAAATTATTGCAGGAAAAATATGTACATCTTTGTGGGCAGATAGAAGATGAGAAATTAGATTCATCAAATGGTATGGCAGACGAAGAAAGTTCAGTGGAAGATGATACAGTTCGTAGTCTACGTACCAGCCCCATCAATCCGTCTTCTAAGGATCGAACATCTATTGAAGATTTTGAAATAATAAAACCAATAAGCAGGGGTGCATTTGGACGAGTTTTTCTTGCCAGAAAACGAGCGACTGGTGACTTATTTGCTATAAAGGTATTAAAGAAGGCTGATATGATTCGTAAGAATGCAGTTGAAAGTATTTTGGCAGAGCGTAACATCCTTATATTGGTTCGCAATCCTTTTGTGGTCCGATTTTTCTATTCCTTCACATGCAGGGAAAATCTTTATCTGGTCATGGAGTACTTAAATGGTGGAGATCTTTACTCTTTATTGAGAAATCTAGGCTGCTTAGATGAAGATATGGCCCGTGTATATATTGCAGAAGTTGTTCTTGCTTTGGAGTATCTGCATTCATTAAATGTTATTCATAGAGACTTGAAGCCAGACAACTTGTTGATTGGTCAAGATGGTCACATCAAGTTGACAGATTTTGGGCTCTCCAAGGTTGGTCTAATCAGCAGCACTGATGACTTAGCAGGTCCATCTGTTGGTGATACTGGTTCTGTTGGAGATGATGAACCAAAAAGTCAATCTTCATCTCAGAGGTCACAGCGTCAAAAACATTCAGTTGTTGGCACCCCCGATTATTTGGCACCTGAAATACTTCTTGGCATGGGACATAGTGCAACTGCTGATTGGTGGTCTGTGGGTGTTATACTTTTTGAGCTACTTGTTGGCATTCCACCATTCAATGCAGAACATCCACAGCAAATTTTCAACAATATAATAAACAGAGATATCCCCTGGCCTAAGGTACCTGAGGAGATGAGCAACGAAGCATATGACTTAATTGACCAATTACTGACTGAAAACCCAGTTCAAAGACTTGGAGCAACAGGTGCTAGGGAGGTGAAGCAACATACTTTTTTCAAGGATATTAACTGGGATACACTTGCAAGGCAGAAGGCTATGTTCATACCTTCAACAGATGCACATGATACAAGTTATTTCATGAGCCGGTACATATGGAATCCAGAAGATGAGCATGTTAATGGAGGCAGTGACTTTGATGACCTGAGTACTGAAACATGCAGCAGTACTGGTTCATATTATCTAGATGAAGACGGGGATGAGTGTGGTAGTTTGGCGGACTTCAGTGCTCCGGCCCTTGATGTGCAGTACTCATTTAGTAATTTCTCATTTAAGAACTTGTCTCAGCTGGCTTCAATCAATTATGATCTTGTCGTGAAGACCAAGGAGTCACCAGAAGCCTCCAAGTCATCTGTTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1176

Amino Acids

131.43

Weight (kDa)

5.2

Isoelectric Point (pI)

49.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	208 - 326	5.1e-56	IREH1-like domain
Pkinase	PF00069	768 - 1056	4.9e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	769 - 915	1.1e-24	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	947 - 1042	5.9e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62310
fragaria_vesca	FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920
malus_domestica	MD00G1104600.v1.1 MD07G1256300.v1.1
prunus_persica	Prupe.2G255800_v2.0.a1 Prupe.2G280200_v2.0.a1 Prupe.2G280200_v2.0.a1
pyrus_communis	pycom01g19590 pycom07g22920
rosa_chinensis	RchiOBHm_Chr1g0374881
rosa_laevigata	RLG00000026710
rosa_multiflora	Rmu_sc0003145.1_g000005 Rmu_sc0004947.1_g000030
rosa_roxburghii	Rroxscaffold_4G00283210
rosa_rugosa	Rorug01G0385500
rosa_samantha	Rh1AG397100 Rh1BG360900 Rh1CG374600 Rh1DG391900
rosa_wichuraiana	Rw1G035210

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3496
Acc36I	ACCTGC	1 cut(s)	2765
Acc65I	GGTACC	1 cut(s)	3052
AccB1I	GGYRCC	4 cut(s)	304, 2873, 2890, 3052
AccB7I	CCANNNNNTGG	1 cut(s)	2786
AccBSI	CCGCTC	1 cut(s)	316
AccI	GTMKAC	2 cut(s)	742, 2248
AclWI	GGATC	4 cut(s)	5, 250, 854, 2289
AcoI	YGGCCR	1 cut(s)	162
AcsI	RAATTY	6 cut(s)	592, 784, 839, 1050, 1420, 3009
AcuI	CTGAAG	4 cut(s)	710, 741, 1575, 3376
AcyI	GRCGYC	2 cut(s)	305, 886
AdeI	CACNNNGTG	1 cut(s)	1091
AfiI	CCNNNNNNNGG	3 cut(s)	1841, 2464, 2786
AflIII	ACRYGT	2 cut(s)	1465, 1803
AjnI	CCWGG	2 cut(s)	2076, 3041
AjuI	GAANNNNNNNTTGG	2 cut(s)	2889, 2921
Alw21I	GWGCWC	6 cut(s)	420, 956, 1002, 1369, 1479, 3400
Alw44I	GTGCAC	3 cut(s)	416, 952, 1365
AlwI	GGATC	4 cut(s)	5, 250, 854, 2289
Ama87I	CYCGRG	1 cut(s)	421
AoxI	GGCC	5 cut(s)	162, 906, 2601, 3044, 3402
ApaI	GGGCCC	1 cut(s)	910
ApaLI	GTGCAC	3 cut(s)	416, 952, 1365
ApeKI	GCWGC	7 cut(s)	266, 1235, 1399, 1625, 2583, 2757, 3330
ApoI	RAATTY	6 cut(s)	592, 784, 839, 1050, 1420, 3009
ArsI	GACNNNNNNTTYG	2 cut(s)	1754, 1786
Asp700I	GAANNNNTTC	2 cut(s)	2625, 2901
Asp718I	GGTACC	1 cut(s)	3052
AspA2I	CCTAGG	2 cut(s)	152, 512
AspLEI	GCGC	2 cut(s)	307, 667
AspS9I	GGNCC	8 cut(s)	906, 907, 1256, 1845, 2485, 2602, 2777, 3403
AsuC2I	CCSGG	2 cut(s)	354, 811
AsuHPI	GGTGA	9 cut(s)	368, 740, 758, 1007, 1701, 2384, 2801, 3160, 3492
AvaI	CYCGRG	1 cut(s)	421
AvaII	GGWCC	4 cut(s)	1256, 1845, 2485, 2777
AvrII	CCTAGG	2 cut(s)	152, 512
AxyI	CCTNAGG	2 cut(s)	3048, 3057
BaeGI	GKGCMC	4 cut(s)	420, 910, 956, 1369
BanI	GGYRCC	4 cut(s)	304, 2873, 2890, 3052
BanII	GRGCYC	4 cut(s)	152, 282, 910, 2736
BarI	GAAGNNNNNNTAC	2 cut(s)	849, 881
BauI	CACGAG	1 cut(s)	1125
BbsI	GAAGAC	8 cut(s)	747, 923, 1004, 2064, 2125, 2265, 3366, 3494
Bbv12I	GWGCWC	6 cut(s)	420, 956, 1002, 1369, 1479, 3400
BbvI	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2570, 2769, 3342
BceAI	ACGGC	2 cut(s)	292, 974
BciT130I	CCWGG	2 cut(s)	2078, 3043
BciVI	GTATCC	5 cut(s)	1315, 1647, 1658, 1694, 3178
BcnI	CCSGG	2 cut(s)	354, 811
BfmI	CTRYAG	2 cut(s)	1201, 2023
BfoI	RGCGCY	2 cut(s)	308, 668
BfuAI	ACCTGC	1 cut(s)	2765
BfuI	GTATCC	5 cut(s)	1315, 1647, 1658, 1694, 3178
BglII	AGATCT	2 cut(s)	1783, 2554
BlnI	CCTAGG	2 cut(s)	152, 512
BmcAI	AGTACT	4 cut(s)	2540, 3319, 3337, 3419
Bme1390I	CCNGG	4 cut(s)	354, 811, 2078, 3043
Bme18I	GGWCC	4 cut(s)	1256, 1845, 2485, 2777
BmeT110I	CYCGRG	1 cut(s)	421
BmgT120I	GGNCC	8 cut(s)	906, 907, 1256, 1845, 2485, 2602, 2777, 3403
BmiI	GGNNCC	8 cut(s)	149, 306, 907, 908, 1846, 2875, 2892, 3054
BmrFI	CCNGG	4 cut(s)	354, 811, 2078, 3043
BmrI	ACTGGG	2 cut(s)	3107, 3190
BmsI	GCATC	6 cut(s)	86, 1090, 1612, 1747, 2083, 3217
BmuI	ACTGGG	2 cut(s)	3107, 3190
BpiI	GAAGAC	8 cut(s)	747, 923, 1004, 2064, 2125, 2265, 3366, 3494
BplI	GAGNNNNNCTC	6 cut(s)	99, 131, 565, 597, 1463, 1495
BpmI	CTGGAG	1 cut(s)	88
Bpu10I	CCTNAGC	1 cut(s)	668
BpuEI	CTTGAG	2 cut(s)	1716, 1973
BpuMI	CCSGG	2 cut(s)	354, 811
BsaAI	YACGTR	2 cut(s)	1468, 2252
BsaBI	GATNNNNATC	1 cut(s)	15
BsaHI	GRCGYC	2 cut(s)	305, 886
BsaI	GGTCTC	1 cut(s)	239
BsaJI	CCNNGG	7 cut(s)	152, 165, 512, 1030, 2739, 3041, 3492
BsaWI	WCCGGW	2 cut(s)	318, 616
Bsc4I	CCNNNNNNNGG	3 cut(s)	1841, 2464, 2786
Bse118I	RCCGGY	3 cut(s)	53, 335, 3255
Bse1I	ACTGG	8 cut(s)	404, 1198, 1986, 2374, 2800, 3113, 3185, 3343
Bse21I	CCTNAGG	2 cut(s)	3048, 3057
Bse8I	GATNNNNATC	1 cut(s)	15
BseBI	CCWGG	2 cut(s)	2078, 3043
BseDI	CCNNGG	7 cut(s)	152, 165, 512, 1030, 2739, 3041, 3492
BseGI	GGATG	8 cut(s)	91, 700, 883, 1212, 2098, 2454, 2998, 3373
BseJI	GATNNNNATC	1 cut(s)	15
BseLI	CCNNNNNNNGG	3 cut(s)	1841, 2464, 2786
BseNI	ACTGG	8 cut(s)	404, 1198, 1986, 2374, 2800, 3113, 3185, 3343
BseRI	GAGGAG	3 cut(s)	665, 1487, 3074
BseSI	GKGCMC	4 cut(s)	420, 910, 956, 1369
BseXI	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2570, 2769, 3342
BsgI	GTGCAG	3 cut(s)	986, 1083, 3434
Bsh1285I	CGRYCG	1 cut(s)	302
BshFI	GGCC	5 cut(s)	164, 908, 2603, 3046, 3404
BshNI	GGYRCC	4 cut(s)	304, 2873, 2890, 3052
BsiEI	CGRYCG	1 cut(s)	302
BsiHKAI	GWGCWC	6 cut(s)	420, 956, 1002, 1369, 1479, 3400
BsiHKCI	CYCGRG	1 cut(s)	421
BsiSI	CCGG	9 cut(s)	54, 145, 319, 336, 354, 617, 811, 3256, 3401
BslFI	GGGAC	3 cut(s)	613, 1858, 2928
BslI	CCNNNNNNNGG	3 cut(s)	1841, 2464, 2786
BsmBI	CGTCTC	3 cut(s)	87, 189, 2102
BsmFI	GGGAC	3 cut(s)	613, 1858, 2928
BsmI	GAATGC	4 cut(s)	635, 2428, 2647, 2976
BsnI	GGCC	5 cut(s)	164, 908, 2603, 3046, 3404
Bso31I	GGTCTC	1 cut(s)	239
BsoBI	CYCGRG	1 cut(s)	421
Bsp120I	GGGCCC	1 cut(s)	906
Bsp1407I	TGTACA	4 cut(s)	865, 1462, 1490, 2152
BspANI	GGCC	5 cut(s)	164, 908, 2603, 3046, 3404
BspHI	TCATGA	3 cut(s)	583, 1339, 3249
BspLI	GGNNCC	8 cut(s)	149, 306, 907, 908, 1846, 2875, 2892, 3054
BspMI	ACCTGC	1 cut(s)	2765
BspPI	GGATC	4 cut(s)	5, 250, 854, 2289
BspQI	GCTCTTC	1 cut(s)	990
BspT107I	GGYRCC	4 cut(s)	304, 2873, 2890, 3052
BspTNI	GGTCTC	1 cut(s)	239
BsrBI	CCGCTC	1 cut(s)	316
BsrFI	RCCGGY	3 cut(s)	53, 335, 3255
BsrGI	TGTACA	4 cut(s)	865, 1462, 1490, 2152
BsrI	ACTGG	8 cut(s)	404, 1198, 1986, 2374, 2800, 3113, 3185, 3343
BssAI	RCCGGY	3 cut(s)	53, 335, 3255
BssECI	CCNNGG	7 cut(s)	152, 165, 512, 1030, 2739, 3041, 3492
BssNAI	GTATAC	1 cut(s)	743
BssNI	GRCGYC	2 cut(s)	305, 886
BssSI	CACGAG	1 cut(s)	1125
BssT1I	CCWWGG	5 cut(s)	152, 512, 1030, 2739, 3492
Bst1107I	GTATAC	1 cut(s)	743
Bst2BI	CACGAG	1 cut(s)	1125
Bst2UI	CCWGG	2 cut(s)	2078, 3043
Bst4CI	ACNGT	4 cut(s)	1462, 1528, 2027, 2239
Bst6I	CTCTTC	4 cut(s)	183, 331, 548, 990
BstACI	GRCGYC	2 cut(s)	305, 886
BstAPI	GCANNNNNTGC	2 cut(s)	1823, 3137
BstAUI	TGTACA	4 cut(s)	865, 1462, 1490, 2152
BstBAI	YACGTR	2 cut(s)	1468, 2252
BstC8I	GCNNGC	5 cut(s)	55, 337, 341, 1305, 3459
BstENI	CCTNNNNNAGG	1 cut(s)	1839
BstF5I	GGATG	8 cut(s)	91, 700, 883, 1212, 2098, 2454, 2998, 3373
BstH2I	RGCGCY	2 cut(s)	308, 668
BstHHI	GCGC	2 cut(s)	307, 667
BstMCI	CGRYCG	1 cut(s)	302
BstMWI	GCNNNNNNNGC	8 cut(s)	59, 68, 313, 671, 892, 1148, 1823, 3137
BstNI	CCWGG	2 cut(s)	2078, 3043
BstNSI	RCATGY	6 cut(s)	1307, 1741, 1807, 2511, 3287, 3330
BstSCI	CCNGG	4 cut(s)	352, 809, 2076, 3041
BstSFI	CTRYAG	2 cut(s)	1201, 2023
BstSLI	GKGCMC	4 cut(s)	420, 910, 956, 1369
BstSNI	TACGTA	1 cut(s)	2252
BstV1I	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2570, 2769, 3342
BstV2I	GAAGAC	8 cut(s)	747, 923, 1004, 2064, 2125, 2265, 3366, 3494
BstX2I	RGATCY	2 cut(s)	1783, 2554
BstYI	RGATCY	2 cut(s)	1783, 2554
BstZ17I	GTATAC	1 cut(s)	743
Bsu36I	CCTNAGG	2 cut(s)	3048, 3057
BsuI	GTATCC	5 cut(s)	1315, 1647, 1658, 1694, 3178
BsuRI	GGCC	5 cut(s)	164, 908, 2603, 3046, 3404
BtgZI	GCGATG	1 cut(s)	435
BtsCI	GGATG	8 cut(s)	91, 700, 883, 1212, 2098, 2454, 2998, 3373
BtsI	GCAGTG	3 cut(s)	821, 1170, 3304
BtsIMutI	CAGTG	9 cut(s)	411, 821, 1030, 1085, 1170, 2229, 2760, 3304, 3400
BveI	ACCTGC	1 cut(s)	2765
Cac8I	GCNNGC	5 cut(s)	55, 337, 341, 1305, 3459
CciI	TCATGA	3 cut(s)	583, 1339, 3249
CfoI	GCGC	2 cut(s)	307, 667
Cfr10I	RCCGGY	3 cut(s)	53, 335, 3255
Cfr13I	GGNCC	8 cut(s)	906, 907, 1256, 1845, 2485, 2602, 2777, 3403
CseI	GACGC	4 cut(s)	258, 875, 1823, 2840
CspCI	CAANNNNNGTGG	2 cut(s)	1894, 1929
DinI	GGCGCC	1 cut(s)	306
DraIII	CACNNNGTG	1 cut(s)	1091
DrdI	GACNNNNNNGTC	1 cut(s)	3496
DseDI	GACNNNNNNGTC	1 cut(s)	3496
EaeI	YGGCCR	1 cut(s)	162
Eam1104I	CTCTTC	4 cut(s)	183, 331, 548, 990
EarI	CTCTTC	4 cut(s)	183, 331, 548, 990
EciI	GGCGGA	3 cut(s)	37, 322, 3401
Eco105I	TACGTA	1 cut(s)	2252
Eco130I	CCWWGG	5 cut(s)	152, 512, 1030, 2739, 3492
Eco24I	GRGCYC	4 cut(s)	152, 282, 910, 2736
Eco31I	GGTCTC	1 cut(s)	239
Eco32I	GATATC	1 cut(s)	3037
Eco47I	GGWCC	4 cut(s)	1256, 1845, 2485, 2777
Eco57I	CTGAAG	4 cut(s)	710, 741, 1575, 3376
Eco81I	CCTNAGG	2 cut(s)	3048, 3057
Eco88I	CYCGRG	1 cut(s)	421
EcoNI	CCTNNNNNAGG	1 cut(s)	1839
EcoO109I	RGGNCCY	3 cut(s)	906, 907, 1256
EcoRII	CCWGG	2 cut(s)	2076, 3041
EcoRV	GATATC	1 cut(s)	3037
EcoT14I	CCWWGG	5 cut(s)	152, 512, 1030, 2739, 3492
EcoT38I	GRGCYC	4 cut(s)	152, 282, 910, 2736
EgeI	GGCGCC	1 cut(s)	306
EheI	GGCGCC	1 cut(s)	306
ErhI	CCWWGG	5 cut(s)	152, 512, 1030, 2739, 3492
Esp3I	CGTCTC	3 cut(s)	87, 189, 2102
FaqI	GGGAC	3 cut(s)	613, 1858, 2928
FauI	CCCGC	3 cut(s)	76, 718, 1855
FauNDI	CATATG	4 cut(s)	645, 1414, 3079, 3263
FblI	GTMKAC	2 cut(s)	742, 2248
FokI	GGATG	8 cut(s)	78, 707, 890, 1219, 2105, 2441, 2985, 3380
FriOI	GRGCYC	4 cut(s)	152, 282, 910, 2736
GlaI	GCGC	2 cut(s)	306, 666
GsuI	CTGGAG	1 cut(s)	88
HaeII	RGCGCY	2 cut(s)	308, 668
HaeIII	GGCC	5 cut(s)	164, 908, 2603, 3046, 3404
HapII	CCGG	9 cut(s)	54, 145, 319, 336, 354, 617, 811, 3256, 3401
HgaI	GACGC	4 cut(s)	258, 875, 1823, 2840
HhaI	GCGC	2 cut(s)	307, 667
Hin1I	GRCGYC	2 cut(s)	305, 886
Hin6I	GCGC	2 cut(s)	305, 665
HinP1I	GCGC	2 cut(s)	305, 665
HincII	GTYRAC	2 cut(s)	172, 2721
HindII	GTYRAC	2 cut(s)	172, 2721
HpaII	CCGG	9 cut(s)	54, 145, 319, 336, 354, 617, 811, 3256, 3401
HphI	GGTGA	9 cut(s)	368, 740, 758, 1007, 1701, 2384, 2801, 3160, 3492
Hpy99I	CGWCG	5 cut(s)	188, 218, 221, 274, 313
HpyCH4III	ACNGT	4 cut(s)	1462, 1528, 2027, 2239
HpyCH4IV	ACGT	5 cut(s)	1467, 1819, 2111, 2122, 2251
HpyF10VI	GCNNNNNNNGC	8 cut(s)	59, 68, 313, 671, 892, 1148, 1823, 3137
HpySE526I	ACGT	5 cut(s)	1467, 1819, 2111, 2122, 2251
Hsp92I	GRCGYC	2 cut(s)	305, 886
HspAI	GCGC	2 cut(s)	305, 665
KasI	GGCGCC	1 cut(s)	304
KpnI	GGTACC	1 cut(s)	3056
KroI	GCCGGC	2 cut(s)	53, 335
KroNI	GCCGGC	2 cut(s)	55, 337
LguI	GCTCTTC	1 cut(s)	990
Lsp1109I	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2570, 2769, 3342
LweI	GCATC	6 cut(s)	86, 1090, 1612, 1747, 2083, 3217
MaeII	ACGT	5 cut(s)	1467, 1819, 2111, 2122, 2251
MbiI	CCGCTC	1 cut(s)	316
MfeI	CAATTG	3 cut(s)	1389, 1494, 2014
MflI	RGATCY	2 cut(s)	1783, 2554
Mly113I	GGCGCC	1 cut(s)	305
MlyI	GAGTC	5 cut(s)	82, 289, 433, 770, 3506
MmeI	TCCRAC	4 cut(s)	110, 450, 855, 2785
MreI	CGCCGGCG	1 cut(s)	335
MroNI	GCCGGC	2 cut(s)	53, 335
MroXI	GAANNNNTTC	2 cut(s)	2625, 2901
MslI	CAYNNNNRTG	3 cut(s)	1302, 2160, 3288
MspA1I	CMGCKG	2 cut(s)	1862, 3457
MspI	CCGG	9 cut(s)	54, 145, 319, 336, 354, 617, 811, 3256, 3401
MspR9I	CCNGG	4 cut(s)	354, 811, 2078, 3043
MunI	CAATTG	3 cut(s)	1389, 1494, 2014
Mva1269I	GAATGC	4 cut(s)	635, 2428, 2647, 2976
MvaI	CCWGG	2 cut(s)	2078, 3043
MwoI	GCNNNNNNNGC	8 cut(s)	59, 68, 313, 671, 892, 1148, 1823, 3137
NaeI	GCCGGC	2 cut(s)	55, 337
NarI	GGCGCC	1 cut(s)	305
NciI	CCSGG	2 cut(s)	354, 811
NdeI	CATATG	4 cut(s)	645, 1414, 3079, 3263
NgoMIV	GCCGGC	2 cut(s)	53, 335
NlaIV	GGNNCC	8 cut(s)	149, 306, 907, 908, 1846, 2875, 2892, 3054
NmeAIII	GCCGAG	1 cut(s)	190
NmuCI	GTSAC	9 cut(s)	1025, 1508, 1811, 2018, 2372, 2708, 2844, 3299, 3498
NspI	RCATGY	6 cut(s)	1307, 1741, 1807, 2511, 3287, 3330
PaeI	GCATGC	1 cut(s)	1307
PaeR7I	CTCGAG	1 cut(s)	421
PagI	TCATGA	3 cut(s)	583, 1339, 3249
PciI	ACATGT	1 cut(s)	1803
PciSI	GCTCTTC	1 cut(s)	990
PcsI	WCGNNNNNNNCGW	3 cut(s)	11, 180, 2248
PctI	GAATGC	4 cut(s)	635, 2428, 2647, 2976
PdiI	GCCGGC	2 cut(s)	55, 337
PdmI	GAANNNNTTC	2 cut(s)	2625, 2901
PfeI	GAWTC	9 cut(s)	16, 124, 139, 1187, 1262, 1577, 2189, 2413, 3268
PflMI	CCANNNNNTGG	1 cut(s)	2786
PfoI	TCCNGGA	1 cut(s)	2076
Ple19I	CGATCG	1 cut(s)	302
PleI	GAGTC	5 cut(s)	82, 289, 432, 769, 3505
PluTI	GGCGCC	1 cut(s)	308
PpsI	GAGTC	5 cut(s)	82, 289, 432, 769, 3505
Ppu21I	YACGTR	2 cut(s)	1468, 2252
PpuMI	RGGWCCY	1 cut(s)	1256
PscI	ACATGT	1 cut(s)	1803
Psp5II	RGGWCCY	1 cut(s)	1256
Psp6I	CCWGG	2 cut(s)	2076, 3041
PspGI	CCWGG	2 cut(s)	2076, 3041
PspN4I	GGNNCC	8 cut(s)	149, 306, 907, 908, 1846, 2875, 2892, 3054
PspOMI	GGGCCC	1 cut(s)	906
PspPI	GGNCC	8 cut(s)	906, 907, 1256, 1845, 2485, 2602, 2777, 3403
PspPPI	RGGWCCY	1 cut(s)	1256
PspXI	VCTCGAGB	1 cut(s)	421
PsuI	RGATCY	2 cut(s)	1783, 2554
PvuI	CGATCG	1 cut(s)	302
PvuII	CAGCTG	1 cut(s)	3457
RseI	CAYNNNNRTG	3 cut(s)	1302, 2160, 3288
SapI	GCTCTTC	1 cut(s)	990
Sau96I	GGNCC	8 cut(s)	906, 907, 1256, 1845, 2485, 2602, 2777, 3403
ScaI	AGTACT	4 cut(s)	2540, 3319, 3337, 3419
SchI	GAGTC	5 cut(s)	82, 289, 433, 770, 3506
ScrFI	CCNGG	4 cut(s)	354, 811, 2078, 3043
SfaNI	GCATC	6 cut(s)	86, 1090, 1612, 1747, 2083, 3217
SfcI	CTRYAG	2 cut(s)	1201, 2023
SfoI	GGCGCC	1 cut(s)	306
Sfr274I	CTCGAG	1 cut(s)	421
SgrAI	CRCCGGYG	1 cut(s)	335
SinI	GGWCC	4 cut(s)	1256, 1845, 2485, 2777
SlaI	CTCGAG	1 cut(s)	421
SmiMI	CAYNNNNRTG	3 cut(s)	1302, 2160, 3288
SmlI	CTYRAG	3 cut(s)	421, 1731, 1952
SmoI	CTYRAG	3 cut(s)	421, 1731, 1952
SnaBI	TACGTA	1 cut(s)	2252
SphI	GCATGC	1 cut(s)	1307
SspDI	GGCGCC	1 cut(s)	304
SspI	AATATT	1 cut(s)	1792
StyD4I	CCNGG	4 cut(s)	352, 809, 2076, 3041
StyI	CCWWGG	5 cut(s)	152, 512, 1030, 2739, 3492
TaaI	ACNGT	4 cut(s)	1462, 1528, 2027, 2239
TaiI	ACGT	5 cut(s)	1470, 1822, 2114, 2125, 2254
TaqI	TCGA	7 cut(s)	5, 41, 298, 422, 1280, 1483, 2284
TaqII	GACCGA	1 cut(s)	2064
TatI	WGTACW	9 cut(s)	865, 1462, 1490, 2027, 2152, 2538, 3317, 3335, 3417
TauI	GCSGC	4 cut(s)	53, 74, 77, 316
TfiI	GAWTC	9 cut(s)	16, 124, 139, 1187, 1262, 1577, 2189, 2413, 3268
TscAI	CASTG	9 cut(s)	411, 821, 1030, 1092, 1177, 2229, 2767, 3304, 3400
TseFI	GTSAC	9 cut(s)	1025, 1508, 1811, 2018, 2372, 2708, 2844, 3299, 3498
TseI	GCWGC	7 cut(s)	266, 1235, 1399, 1625, 2583, 2757, 3330
Tsp45I	GTSAC	9 cut(s)	1025, 1508, 1811, 2018, 2372, 2708, 2844, 3299, 3498
TspGWI	ACGGA	3 cut(s)	107, 307, 2259
TspRI	CASTG	9 cut(s)	411, 821, 1030, 1092, 1177, 2229, 2767, 3304, 3400
Van91I	CCANNNNNTGG	1 cut(s)	2786
VneI	GTGCAC	3 cut(s)	416, 952, 1365
VpaK11BI	GGWCC	4 cut(s)	1256, 1845, 2485, 2777
XagI	CCTNNNNNAGG	1 cut(s)	1839
XapI	RAATTY	6 cut(s)	592, 784, 839, 1050, 1420, 3009
XbaI	TCTAGA	1 cut(s)	3352
XceI	RCATGY	6 cut(s)	1307, 1741, 1807, 2511, 3287, 3330
XcmI	CCANNNNNNNNNTGG	3 cut(s)	618, 980, 1315
XhoI	CTCGAG	1 cut(s)	421
XmaJI	CCTAGG	2 cut(s)	152, 512
XmiI	GTMKAC	2 cut(s)	742, 2248
XmnI	GAANNNNTTC	2 cut(s)	2625, 2901
ZrmI	AGTACT	4 cut(s)	2540, 3319, 3337, 3419

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.