Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0008055

Physical Location & Seq

Genomic Coordinates Forward (+)

5180817 .. 5187533

Gene Structure 6717 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00283210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3216 bp

ATGTGCAGTGGGCTTCATGATCCGTTTATTTCCAAGGAACGTAGAGAGACCACGCCGATCCTATTGGCTGCGTCGCTAGGGCTCAATCACATACGGACTCGATCGGCGCCGTCGCCGCTCCGGTTCTCTTCCTCCGCCGGCGCACCTCTAATTCCCGGCGAGGAAGCGAATAATATGAACTACACGGTCAAGTCAAAGCCCCAACCAGTGAAATATGTGCACTCGAGTCAATCAAAATCGCTGAGAACTCCATCGCCACTCAATCCTGTGTTGGAGGGTAGTCAAGCTGGTCTTACAAAGGAAATGCAGTCGCCTCGGTTTCAGGCAATACTGCGGGTTACGAGTGGACGAAAGAAGAGAACACCTGACATCAAGAGTTTCTCTCATGAACTTAATTCCAAAGGAGTTAGACCATTTCCGGTGTGGAAGTCCCGTGCATTCGGGCATATGGAGGTGACATTACCTTCACCTGTGTATGAGGTTTTGGTGGCGCTCAGAGCAAGATTTGAGAGGTTGAAGGATGAGGTTGATGTTGATTTAGGTGCATTTGCGGGTGATTTGGTGGGTATACTTGAAAAGTCTTCAGAGTCTCACCCTGAATGGAAAGAGAATTTTGAGGATTTGCTAGTTGTTGCCCGGCAGTGTGCAAAGATGTCTCCTAACGAATTTTGGATCAAGTGTGAAGGCATTGTACAGAAGTTGGATGACCAGCGTCAAGAGCTACCAATGGGGGCCCTGAAACAAGCCCATACTCGTCTTCTTTTTATTCTTACTCGGTGCACACGGCTTGTGCAGTTCCAGAAAGAGAGTGGTTATGAGGAAGAGCACATTCTTAGTCTTCACCAGCTCAGTGACCTTGGTGTTTATCCAGAACAAATTTTGGAGGGTGCAGAACAGAAGTTTAGTGGTCCACTGAGTGGGAAAGATGCAAACGATAAGCCCAAGAAGTCTCGTGAGCAAGAAATAGCTAGTGGAGCTGATGGTGTGGAGGTTAGCACTGCCAAGAGCGTTGAATCTACTGGTAGCTATAGGATGTCATCTTGGAAAAAACTTCCATCGGCTGCTGAGAAAAAGCAGAAAGTACCTGATTCTGTTGATACACCATCGAAGGACAAATCAGAATGTTTGCATGCCAAAGAAAATCTGGATACTCCATCATCCCATCATGAACACTCAGAGATGTCCACAAGTGCACAAAGAGTATCTTGGGTTTCAATTGGGGAGCAGCAAAATGTTACATATGAGAATTTGATGATCTGTCGGATATGTGAGGTTGAAATACCAACTGTACACGTAGAGGAGCACTCTCGAATATGTACAATTGCTGACAGATGTGACTTGAAGGGCTTAACTGTGAATGAAAGACTTGAAAGAGTTGCTGAAGCTCTTGAAAGGATAATGGAATCTCGGACAACAAAAAATGTTGATACACAAGAGACAAGTTTTGATGCTGCAAAAGTGTATCCATCAAATCTTAATATTATGCCTGACATGTCATGTGATGCACGTTCTGTTTTGACACCTGAACAAGGAACCAGAACCTCATCAGCGGGAAGCTCCACTCCAAGATCGCCACTTTTAACACCTAGAACCACTCATATTGAAATGCTGTTGAGTGGAAGGGGAACAATACCAGAACTTGAGAATTGTCAGCAGATACACAAGCTACTGGATATTGCTCGTTCTGTTGCAAATGTTAGCAATTGTGACTATAGTGCATTGGAGTATATGCTTGACCGACTAGAAGACCTAAAATATGCTATCCAGGACAGGAAGGTGGATGCCCTTGTTGTAGAGACGTTTGGAAGGCGTATTGAAAAATTATTGCAGGAAAAATATGTACATCTTTGTGGGCAGATAGAAGATGAGAAATTGGATTCATCAAATGGTATGGCAGACGAAGAAAGTTCAGTGGAAGATGATACTGTTCGTAGTCTACGCACCAGCCCCATCAATCCATCTTCTAAGGATCGAACATCTATTGAAGATTTTGAAATAATAAAACCAATAAGCAGGGGTGCATTTGGACGAGTTTTTCTTGCCAGAAAACGAGCGACTGGTGACTTATTTGCTATAAAGGTATTAAAGAAGGCAGATATGATTCGTAAGAATGCAGTTGAAAGTATTTTGGCAGAGCGTAACATCCTTATATTGGTTCGCAATCCTTTTGTGGTCCGATTTTTCTATTCCTTCACATGCAGGGAAAATCTTTATCTGGTCATGGAGTACTTAAATGGTGGAGATCTTTACTCTTTATTGAGAAATCTAGGCTGCTTAGATGAAGATATGGCCCGTGTATATATTGCAGAAGTTGTTCTTGCTTTGGAGTATCTGCATTCATTAAATGTTATTCATAGAGACTTGAAGCCAGACAACTTGTTGATTGGTCAAGATGGTCACATCAAGTTGACAGATTTTGGGCTCTCCAAGGTTGGTCTAATCAGCAGCACTGATGACTTAGCAGGTCCATCTGTTGGTGAGACTGGTTTTGTTGGAGACGATGAACCAAAAAGTCAATCTTCATCTCAGAGGTCACAGCGTCAAAAACATTCAGTTGTTGGCACCCCCGATTATTTGGCACCTGAAATACTTCTTGGCATGGGACATAGTGCAACTGCTGATTGGTGGTCTGTGGGTGTTATACTTTTTGAGCTACTTGTTGGCATTCCACCATTCAATGCAGAACATCCACAGCAAATTTTCAACAATATAATAAACAGAGATATCCCCTGGCCTAAGGTACCTGAGGAGATGAGCAACGAAGCATATGACTTAATTGACCAATTACTGACTGAAAACCCAGTTCAAAGACTTGGAGCAACAGGTGCTAGGGAGGTGAAGCAACATACTTTTTTCAAGGATATTAACTGGGATACACTTGCAAGGCAGAAGGCTATGTTCATACCTTCAACAGATGCACATGATACAAGTTATTTCATGAGCCGGTACATATGGAATCCAGACGATGAGCATGTTAATGGAGGCAGTGACTTTGATGACCTGACTGAAACATGCAGCAGTACTGGTTCATATTATCTAGACGAAGACGGGGATGAGTGTGGTAGTTTGGCGGACTTCAGTGCTCCGGCCCTTGATGTGCAGTACTCATTTAGTAATTTCTCATTTAAGAACTTGTCTCAGCTGGCTTCCATCAATTATGATCTCGTCGTGAAGACCAAGGAGTCACCAGAAGCCTCCCAGTCATCTGTTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1071

Amino Acids

119.78

Weight (kDa)

5.43

Isoelectric Point (pI)

47.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	158 - 268	9.6e-53	IREH1-like domain
Pkinase	PF00069	664 - 952	4.2e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	665 - 811	9.3e-25	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	843 - 938	5.6e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62310
fragaria_vesca	FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920 FvH4_7g27920
malus_domestica	MD00G1104600.v1.1 MD07G1256300.v1.1
prunus_persica	Prupe.2G255800_v2.0.a1 Prupe.2G280200_v2.0.a1 Prupe.2G280200_v2.0.a1
pyrus_communis	pycom01g19590 pycom07g22920
rosa_chinensis	RchiOBHm_Chr1g0374881
rosa_laevigata	RLG00000026710
rosa_multiflora	Rmu_sc0003145.1_g000005 Rmu_sc0004947.1_g000030
rosa_roxburghii	Rroxscaffold_4G00283210
rosa_rugosa	Rorug01G0385500
rosa_samantha	Rh1AG397100 Rh1BG360900 Rh1CG374600 Rh1DG391900
rosa_wichuraiana	Rw1G035210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3181
Acc36I	ACCTGC	1 cut(s)	2453
Acc65I	GGTACC	1 cut(s)	2740
AccB1I	GGYRCC	4 cut(s)	106, 2561, 2578, 2740
AccB7I	CCANNNNNTGG	2 cut(s)	917, 2474
AccBSI	CCGCTC	1 cut(s)	118
AccI	GTMKAC	2 cut(s)	568, 1936
AciI	CCGC	6 cut(s)	116, 135, 334, 551, 1550, 3071
AclWI	GGATC	4 cut(s)	14, 52, 680, 1977
AcsI	RAATTY	5 cut(s)	610, 665, 876, 1246, 2697
AcuI	CTGAAG	3 cut(s)	567, 1401, 3061
AcyI	GRCGYC	1 cut(s)	107
AdeI	CACNNNGTG	1 cut(s)	917
AfiI	CCNNNNNNNGG	4 cut(s)	917, 1529, 2152, 2474
AflIII	ACRYGT	2 cut(s)	1291, 1491
AjnI	CCWGG	2 cut(s)	1764, 2729
AjuI	GAANNNNNNNTTGG	2 cut(s)	2577, 2609
Alw21I	GWGCWC	6 cut(s)	222, 782, 828, 1195, 1305, 3085
Alw44I	GTGCAC	3 cut(s)	218, 778, 1191
AlwI	GGATC	4 cut(s)	14, 52, 680, 1977
Ama87I	CYCGRG	1 cut(s)	223
AoxI	GGCC	4 cut(s)	732, 2289, 2732, 3087
ApaI	GGGCCC	1 cut(s)	736
ApaLI	GTGCAC	3 cut(s)	218, 778, 1191
ApeKI	GCWGC	7 cut(s)	68, 1061, 1225, 1451, 2271, 2445, 3015
ApoI	RAATTY	5 cut(s)	610, 665, 876, 1246, 2697
Asp700I	GAANNNNTTC	2 cut(s)	2313, 2589
Asp718I	GGTACC	1 cut(s)	2740
AspLEI	GCGC	3 cut(s)	109, 143, 493
AspS9I	GGNCC	7 cut(s)	732, 733, 908, 2173, 2290, 2465, 3088
AsuC2I	CCSGG	2 cut(s)	156, 637
AsuHPI	GGTGA	9 cut(s)	459, 466, 566, 584, 833, 2072, 2489, 2848, 3177
AvaI	CYCGRG	1 cut(s)	223
AvaII	GGWCC	3 cut(s)	908, 2173, 2465
AxyI	CCTNAGG	2 cut(s)	2736, 2745
BaeGI	GKGCMC	4 cut(s)	222, 736, 782, 1195
BanI	GGYRCC	4 cut(s)	106, 2561, 2578, 2740
BanII	GRGCYC	3 cut(s)	84, 736, 2424
BarI	GAAGNNNNNNTAC	2 cut(s)	675, 707
BauI	CACGAG	1 cut(s)	951
BbsI	GAAGAC	6 cut(s)	573, 749, 830, 1752, 3051, 3179
Bbv12I	GWGCWC	6 cut(s)	222, 782, 828, 1195, 1305, 3085
BbvI	GCAGC	7 cut(s)	55, 1048, 1237, 1438, 2258, 2457, 3027
BceAI	ACGGC	2 cut(s)	94, 800
BcgI	CGANNNNNNTGC	2 cut(s)	296, 330
BciT130I	CCWGG	2 cut(s)	1766, 2731
BciVI	GTATCC	3 cut(s)	1141, 1473, 2866
BcnI	CCSGG	2 cut(s)	156, 637
BfaI	CTAG	8 cut(s)	77, 626, 969, 1587, 1742, 2267, 2829, 3038
BfmI	CTRYAG	2 cut(s)	1027, 1711
BfoI	RGCGCY	2 cut(s)	110, 494
BfuAI	ACCTGC	1 cut(s)	2453
BfuI	GTATCC	3 cut(s)	1141, 1473, 2866
BglII	AGATCT	1 cut(s)	2242
BisI	GCNGC	8 cut(s)	69, 116, 1062, 1226, 1452, 2272, 2446, 3016
BlsI	GCNGC	8 cut(s)	70, 117, 1063, 1227, 1453, 2273, 2447, 3017
BmcAI	AGTACT	3 cut(s)	2228, 3022, 3104
Bme1390I	CCNGG	4 cut(s)	156, 637, 1766, 2731
Bme18I	GGWCC	3 cut(s)	908, 2173, 2465
BmeT110I	CYCGRG	1 cut(s)	223
BmgT120I	GGNCC	7 cut(s)	732, 733, 908, 2173, 2290, 2465, 3088
BmiI	GGNNCC	7 cut(s)	108, 733, 734, 1534, 2563, 2580, 2742
BmrFI	CCNGG	4 cut(s)	156, 637, 1766, 2731
BmrI	ACTGGG	3 cut(s)	2795, 2878, 3193
BmsI	GCATC	5 cut(s)	916, 1438, 1492, 1771, 2905
BmuI	ACTGGG	3 cut(s)	2795, 2878, 3193
BoxI	GACNNNNGTC	1 cut(s)	711
BpiI	GAAGAC	6 cut(s)	573, 749, 830, 1752, 3051, 3179
BplI	GAGNNNNNCTC	4 cut(s)	367, 399, 1289, 1321
BpuEI	CTTGAG	1 cut(s)	1661
BpuMI	CCSGG	2 cut(s)	156, 637
BsaAI	YACGTR	1 cut(s)	1294
BsaHI	GRCGYC	1 cut(s)	107
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	6 cut(s)	33, 314, 856, 2427, 2729, 3177
BsaWI	WCCGGW	2 cut(s)	120, 418
Bsc4I	CCNNNNNNNGG	4 cut(s)	917, 1529, 2152, 2474
Bse118I	RCCGGY	2 cut(s)	137, 2943
Bse1I	ACTGG	9 cut(s)	206, 1024, 1674, 2062, 2488, 2801, 2873, 3028, 3199
Bse21I	CCTNAGG	2 cut(s)	2736, 2745
BseBI	CCWGG	2 cut(s)	1766, 2731
BseDI	CCNNGG	6 cut(s)	33, 314, 856, 2427, 2729, 3177
BseGI	GGATG	8 cut(s)	526, 709, 1038, 1157, 1786, 2142, 2686, 3058
BseLI	CCNNNNNNNGG	4 cut(s)	917, 1529, 2152, 2474
BseMII	CTCAG	9 cut(s)	233, 508, 862, 905, 1056, 1188, 2540, 2736, 3152
BseNI	ACTGG	9 cut(s)	206, 1024, 1674, 2062, 2488, 2801, 2873, 3028, 3199
BseRI	GAGGAG	2 cut(s)	1313, 2762
BseSI	GKGCMC	4 cut(s)	222, 736, 782, 1195
BseXI	GCAGC	7 cut(s)	55, 1048, 1237, 1438, 2258, 2457, 3027
BsgI	GTGCAG	4 cut(s)	25, 812, 909, 3119
Bsh1285I	CGRYCG	1 cut(s)	104
BshFI	GGCC	4 cut(s)	734, 2291, 2734, 3089
BshNI	GGYRCC	4 cut(s)	106, 2561, 2578, 2740
BsiEI	CGRYCG	1 cut(s)	104
BsiHKAI	GWGCWC	6 cut(s)	222, 782, 828, 1195, 1305, 3085
BsiHKCI	CYCGRG	1 cut(s)	223
BsiSI	CCGG	7 cut(s)	121, 138, 156, 419, 637, 2944, 3086
BslFI	GGGAC	2 cut(s)	415, 2616
BslI	CCNNNNNNNGG	4 cut(s)	917, 1529, 2152, 2474
BsmBI	CGTCTC	2 cut(s)	1790, 2490
BsmFI	GGGAC	2 cut(s)	415, 2616
BsmI	GAATGC	4 cut(s)	437, 2116, 2335, 2664
BsnI	GGCC	4 cut(s)	734, 2291, 2734, 3089
Bso31I	GGTCTC	1 cut(s)	41
BsoBI	CYCGRG	1 cut(s)	223
Bsp120I	GGGCCC	1 cut(s)	732
Bsp1286I	GDGCHC	9 cut(s)	84, 222, 736, 782, 828, 1195, 1305, 2424, 3085
Bsp1407I	TGTACA	4 cut(s)	691, 1288, 1316, 1840
Bsp143I	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
BspACI	CCGC	6 cut(s)	116, 135, 334, 551, 1550, 3071
BspANI	GGCC	4 cut(s)	734, 2291, 2734, 3089
BspCNI	CTCAG	9 cut(s)	234, 507, 861, 906, 1057, 1187, 2539, 2737, 3151
BspHI	TCATGA	4 cut(s)	16, 385, 1165, 2937
BspLI	GGNNCC	7 cut(s)	108, 733, 734, 1534, 2563, 2580, 2742
BspMI	ACCTGC	1 cut(s)	2453
BspPI	GGATC	4 cut(s)	14, 52, 680, 1977
BspQI	GCTCTTC	1 cut(s)	816
BspT107I	GGYRCC	4 cut(s)	106, 2561, 2578, 2740
BspTNI	GGTCTC	1 cut(s)	41
BsrBI	CCGCTC	1 cut(s)	118
BsrFI	RCCGGY	2 cut(s)	137, 2943
BsrGI	TGTACA	4 cut(s)	691, 1288, 1316, 1840
BsrI	ACTGG	9 cut(s)	206, 1024, 1674, 2062, 2488, 2801, 2873, 3028, 3199
BssAI	RCCGGY	2 cut(s)	137, 2943
BssECI	CCNNGG	6 cut(s)	33, 314, 856, 2427, 2729, 3177
BssMI	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
BssNAI	GTATAC	1 cut(s)	569
BssNI	GRCGYC	1 cut(s)	107
BssSI	CACGAG	1 cut(s)	951
BssT1I	CCWWGG	4 cut(s)	33, 856, 2427, 3177
Bst1107I	GTATAC	1 cut(s)	569
Bst2BI	CACGAG	1 cut(s)	951
Bst2UI	CCWGG	2 cut(s)	1766, 2731
Bst4CI	ACNGT	4 cut(s)	187, 1288, 1354, 1927
Bst6I	CTCTTC	3 cut(s)	133, 350, 816
BstACI	GRCGYC	1 cut(s)	107
BstAPI	GCANNNNNTGC	1 cut(s)	2825
BstAUI	TGTACA	4 cut(s)	691, 1288, 1316, 1840
BstBAI	YACGTR	1 cut(s)	1294
BstC8I	GCNNGC	3 cut(s)	139, 1131, 3144
BstENI	CCTNNNNNAGG	1 cut(s)	1527
BstF5I	GGATG	8 cut(s)	526, 709, 1038, 1157, 1786, 2142, 2686, 3058
BstH2I	RGCGCY	2 cut(s)	110, 494
BstHHI	GCGC	3 cut(s)	109, 143, 493
BstKTI	GATC	9 cut(s)	22, 60, 104, 675, 1257, 1571, 1972, 2245, 3163
BstMBI	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
BstMCI	CGRYCG	1 cut(s)	104
BstMWI	GCNNNNNNNGC	5 cut(s)	115, 497, 718, 974, 2825
BstNI	CCWGG	2 cut(s)	1766, 2731
BstNSI	RCATGY	5 cut(s)	1133, 1495, 2199, 2975, 3015
BstPAI	GACNNNNGTC	1 cut(s)	711
BstSCI	CCNGG	4 cut(s)	154, 635, 1764, 2729
BstSFI	CTRYAG	2 cut(s)	1027, 1711
BstSLI	GKGCMC	4 cut(s)	222, 736, 782, 1195
BstV1I	GCAGC	7 cut(s)	55, 1048, 1237, 1438, 2258, 2457, 3027
BstV2I	GAAGAC	6 cut(s)	573, 749, 830, 1752, 3051, 3179
BstX2I	RGATCY	1 cut(s)	2242
BstYI	RGATCY	1 cut(s)	2242
BstZ17I	GTATAC	1 cut(s)	569
Bsu36I	CCTNAGG	2 cut(s)	2736, 2745
BsuI	GTATCC	3 cut(s)	1141, 1473, 2866
BsuRI	GGCC	4 cut(s)	734, 2291, 2734, 3089
BtgZI	GCGATG	1 cut(s)	237
BtsCI	GGATG	8 cut(s)	526, 709, 1038, 1157, 1786, 2142, 2686, 3058
BtsI	GCAGTG	4 cut(s)	13, 647, 996, 2992
BveI	ACCTGC	1 cut(s)	2453
Cac8I	GCNNGC	3 cut(s)	139, 1131, 3144
CciI	TCATGA	4 cut(s)	16, 385, 1165, 2937
CfoI	GCGC	3 cut(s)	109, 143, 493
Cfr10I	RCCGGY	2 cut(s)	137, 2943
Cfr13I	GGNCC	7 cut(s)	732, 733, 908, 2173, 2290, 2465, 3088
CseI	GACGC	3 cut(s)	60, 701, 2528
CspCI	CAANNNNNGTGG	2 cut(s)	1582, 1617
DinI	GGCGCC	1 cut(s)	108
DpnI	GATC	9 cut(s)	21, 59, 103, 674, 1256, 1570, 1971, 2244, 3162
DpnII	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
DraIII	CACNNNGTG	1 cut(s)	917
DrdI	GACNNNNNNGTC	1 cut(s)	3181
DseDI	GACNNNNNNGTC	1 cut(s)	3181
Eam1104I	CTCTTC	3 cut(s)	133, 350, 816
EarI	CTCTTC	3 cut(s)	133, 350, 816
EciI	GGCGGA	2 cut(s)	124, 3086
Eco130I	CCWWGG	4 cut(s)	33, 856, 2427, 3177
Eco24I	GRGCYC	3 cut(s)	84, 736, 2424
Eco31I	GGTCTC	1 cut(s)	41
Eco32I	GATATC	1 cut(s)	2725
Eco47I	GGWCC	3 cut(s)	908, 2173, 2465
Eco57I	CTGAAG	3 cut(s)	567, 1401, 3061
Eco81I	CCTNAGG	2 cut(s)	2736, 2745
Eco88I	CYCGRG	1 cut(s)	223
EcoNI	CCTNNNNNAGG	1 cut(s)	1527
EcoO109I	RGGNCCY	2 cut(s)	732, 733
EcoRII	CCWGG	2 cut(s)	1764, 2729
EcoRV	GATATC	1 cut(s)	2725
EcoT14I	CCWWGG	4 cut(s)	33, 856, 2427, 3177
EcoT38I	GRGCYC	3 cut(s)	84, 736, 2424
EgeI	GGCGCC	1 cut(s)	108
EheI	GGCGCC	1 cut(s)	108
ErhI	CCWWGG	4 cut(s)	33, 856, 2427, 3177
Esp3I	CGTCTC	2 cut(s)	1790, 2490
FalI	AAGNNNNNCTT	8 cut(s)	276, 308, 1189, 1221, 2301, 2333, 2503, 2535
FaqI	GGGAC	2 cut(s)	415, 2616
FauI	CCCGC	3 cut(s)	327, 544, 1543
FauNDI	CATATG	4 cut(s)	447, 1240, 2767, 2951
FblI	GTMKAC	2 cut(s)	568, 1936
Fnu4HI	GCNGC	8 cut(s)	69, 116, 1062, 1226, 1452, 2272, 2446, 3016
FokI	GGATG	8 cut(s)	533, 716, 1045, 1144, 1793, 2129, 2673, 3065
FriOI	GRGCYC	3 cut(s)	84, 736, 2424
Fsp4HI	GCNGC	8 cut(s)	69, 116, 1062, 1226, 1452, 2272, 2446, 3016
FspBI	CTAG	8 cut(s)	77, 626, 969, 1587, 1742, 2267, 2829, 3038
GlaI	GCGC	3 cut(s)	108, 142, 492
GluI	GCNGC	8 cut(s)	69, 116, 1062, 1226, 1452, 2272, 2446, 3016
HaeII	RGCGCY	2 cut(s)	110, 494
HaeIII	GGCC	4 cut(s)	734, 2291, 2734, 3089
HapII	CCGG	7 cut(s)	121, 138, 156, 419, 637, 2944, 3086
HgaI	GACGC	3 cut(s)	60, 701, 2528
HhaI	GCGC	3 cut(s)	109, 143, 493
Hin1I	GRCGYC	1 cut(s)	107
Hin6I	GCGC	3 cut(s)	107, 141, 491
HinP1I	GCGC	3 cut(s)	107, 141, 491
HincII	GTYRAC	1 cut(s)	2409
HindII	GTYRAC	1 cut(s)	2409
HpaII	CCGG	7 cut(s)	121, 138, 156, 419, 637, 2944, 3086
HphI	GGTGA	9 cut(s)	459, 466, 566, 584, 833, 2072, 2489, 2848, 3177
Hpy188I	TCNGA	8 cut(s)	497, 586, 1120, 1177, 1263, 1410, 2177, 2529
Hpy99I	CGWCG	3 cut(s)	76, 115, 3170
HpyCH4III	ACNGT	4 cut(s)	187, 1288, 1354, 1927
HpyCH4IV	ACGT	4 cut(s)	40, 1293, 1507, 1799
HpyF10VI	GCNNNNNNNGC	5 cut(s)	115, 497, 718, 974, 2825
HpySE526I	ACGT	4 cut(s)	40, 1293, 1507, 1799
Hsp92I	GRCGYC	1 cut(s)	107
HspAI	GCGC	3 cut(s)	107, 141, 491
KasI	GGCGCC	1 cut(s)	106
KpnI	GGTACC	1 cut(s)	2744
KroI	GCCGGC	1 cut(s)	137
KroNI	GCCGGC	1 cut(s)	139
Kzo9I	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
LguI	GCTCTTC	1 cut(s)	816
LmnI	GCTCC	7 cut(s)	123, 974, 1222, 1300, 1562, 2816, 3088
Lsp1109I	GCAGC	7 cut(s)	55, 1048, 1237, 1438, 2258, 2457, 3027
LweI	GCATC	5 cut(s)	916, 1438, 1492, 1771, 2905
MaeI	CTAG	8 cut(s)	77, 626, 969, 1587, 1742, 2267, 2829, 3038
MaeII	ACGT	4 cut(s)	40, 1293, 1507, 1799
MalI	GATC	9 cut(s)	21, 59, 103, 674, 1256, 1570, 1971, 2244, 3162
MbiI	CCGCTC	1 cut(s)	118
MboI	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
MfeI	CAATTG	3 cut(s)	1215, 1320, 1702
MflI	RGATCY	1 cut(s)	2242
MhlI	GDGCHC	9 cut(s)	84, 222, 736, 782, 828, 1195, 1305, 2424, 3085
Mly113I	GGCGCC	1 cut(s)	107
MlyI	GAGTC	4 cut(s)	91, 235, 596, 3191
MmeI	TCCRAC	4 cut(s)	252, 681, 1241, 2473
MreI	CGCCGGCG	1 cut(s)	137
MroNI	GCCGGC	1 cut(s)	137
MroXI	GAANNNNTTC	2 cut(s)	2313, 2589
MslI	CAYNNNNRTG	2 cut(s)	1848, 2976
MspA1I	CMGCKG	2 cut(s)	1550, 3142
MspI	CCGG	7 cut(s)	121, 138, 156, 419, 637, 2944, 3086
MspR9I	CCNGG	4 cut(s)	156, 637, 1766, 2731
MunI	CAATTG	3 cut(s)	1215, 1320, 1702
Mva1269I	GAATGC	4 cut(s)	437, 2116, 2335, 2664
MvaI	CCWGG	2 cut(s)	1766, 2731
MwoI	GCNNNNNNNGC	5 cut(s)	115, 497, 718, 974, 2825
NaeI	GCCGGC	1 cut(s)	139
NarI	GGCGCC	1 cut(s)	107
NciI	CCSGG	2 cut(s)	156, 637
NdeI	CATATG	4 cut(s)	447, 1240, 2767, 2951
NdeII	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
NgoMIV	GCCGGC	1 cut(s)	137
NlaIV	GGNNCC	7 cut(s)	108, 733, 734, 1534, 2563, 2580, 2742
NmuCI	GTSAC	9 cut(s)	454, 851, 1334, 1706, 2060, 2396, 2532, 2987, 3183
NspI	RCATGY	5 cut(s)	1133, 1495, 2199, 2975, 3015
PaeI	GCATGC	1 cut(s)	1133
PaeR7I	CTCGAG	1 cut(s)	223
PagI	TCATGA	4 cut(s)	16, 385, 1165, 2937
PciI	ACATGT	1 cut(s)	1491
PciSI	GCTCTTC	1 cut(s)	816
PctI	GAATGC	4 cut(s)	437, 2116, 2335, 2664
PdiI	GCCGGC	1 cut(s)	139
PdmI	GAANNNNTTC	2 cut(s)	2313, 2589
PfeI	GAWTC	6 cut(s)	1013, 1088, 1403, 1877, 2101, 2956
PflMI	CCANNNNNTGG	2 cut(s)	917, 2474
PfoI	TCCNGGA	1 cut(s)	1764
PkrI	GCNGC	8 cut(s)	70, 117, 1063, 1227, 1453, 2273, 2447, 3017
Ple19I	CGATCG	1 cut(s)	104
PleI	GAGTC	4 cut(s)	91, 234, 595, 3190
PluTI	GGCGCC	1 cut(s)	110
PpsI	GAGTC	4 cut(s)	91, 234, 595, 3190
Ppu21I	YACGTR	1 cut(s)	1294
PscI	ACATGT	1 cut(s)	1491
PshAI	GACNNNNGTC	1 cut(s)	711
Psp6I	CCWGG	2 cut(s)	1764, 2729
PspGI	CCWGG	2 cut(s)	1764, 2729
PspN4I	GGNNCC	7 cut(s)	108, 733, 734, 1534, 2563, 2580, 2742
PspOMI	GGGCCC	1 cut(s)	732
PspPI	GGNCC	7 cut(s)	732, 733, 908, 2173, 2290, 2465, 3088
PspXI	VCTCGAGB	1 cut(s)	223
PsuI	RGATCY	1 cut(s)	2242
PvuI	CGATCG	1 cut(s)	104
PvuII	CAGCTG	1 cut(s)	3142
RseI	CAYNNNNRTG	2 cut(s)	1848, 2976
SapI	GCTCTTC	1 cut(s)	816
SatI	GCNGC	8 cut(s)	69, 116, 1062, 1226, 1452, 2272, 2446, 3016
Sau3AI	GATC	9 cut(s)	19, 57, 101, 672, 1254, 1568, 1969, 2242, 3160
Sau96I	GGNCC	7 cut(s)	732, 733, 908, 2173, 2290, 2465, 3088
ScaI	AGTACT	3 cut(s)	2228, 3022, 3104
SchI	GAGTC	4 cut(s)	91, 235, 596, 3191
ScrFI	CCNGG	4 cut(s)	156, 637, 1766, 2731
SduI	GDGCHC	9 cut(s)	84, 222, 736, 782, 828, 1195, 1305, 2424, 3085
SfaNI	GCATC	5 cut(s)	916, 1438, 1492, 1771, 2905
SfcI	CTRYAG	2 cut(s)	1027, 1711
SfoI	GGCGCC	1 cut(s)	108
Sfr274I	CTCGAG	1 cut(s)	223
SgrAI	CRCCGGYG	1 cut(s)	137
SinI	GGWCC	3 cut(s)	908, 2173, 2465
SlaI	CTCGAG	1 cut(s)	223
SmiMI	CAYNNNNRTG	2 cut(s)	1848, 2976
SmlI	CTYRAG	2 cut(s)	223, 1640
SmoI	CTYRAG	2 cut(s)	223, 1640
SphI	GCATGC	1 cut(s)	1133
SsiI	CCGC	6 cut(s)	116, 135, 334, 551, 1550, 3071
SspDI	GGCGCC	1 cut(s)	106
SspI	AATATT	1 cut(s)	1480
SspMI	CTAG	8 cut(s)	77, 626, 969, 1587, 1742, 2267, 2829, 3038
StyD4I	CCNGG	4 cut(s)	154, 635, 1764, 2729
StyI	CCWWGG	4 cut(s)	33, 856, 2427, 3177
TaaI	ACNGT	4 cut(s)	187, 1288, 1354, 1927
TaiI	ACGT	4 cut(s)	43, 1296, 1510, 1802
TaqI	TCGA	5 cut(s)	100, 224, 1106, 1309, 1972
TaqII	GACCGA	1 cut(s)	1752
TatI	WGTACW	7 cut(s)	691, 1288, 1316, 1840, 2226, 3020, 3102
TauI	GCSGC	1 cut(s)	118
TfiI	GAWTC	6 cut(s)	1013, 1088, 1403, 1877, 2101, 2956
TseFI	GTSAC	9 cut(s)	454, 851, 1334, 1706, 2060, 2396, 2532, 2987, 3183
TseI	GCWGC	7 cut(s)	68, 1061, 1225, 1451, 2271, 2445, 3015
Tsp45I	GTSAC	9 cut(s)	454, 851, 1334, 1706, 2060, 2396, 2532, 2987, 3183
TspGWI	ACGGA	2 cut(s)	12, 109
Van91I	CCANNNNNTGG	2 cut(s)	917, 2474
VneI	GTGCAC	3 cut(s)	218, 778, 1191
VpaK11BI	GGWCC	3 cut(s)	908, 2173, 2465
XagI	CCTNNNNNAGG	1 cut(s)	1527
XapI	RAATTY	5 cut(s)	610, 665, 876, 1246, 2697
XbaI	TCTAGA	1 cut(s)	3037
XceI	RCATGY	5 cut(s)	1133, 1495, 2199, 2975, 3015
XcmI	CCANNNNNNNNNTGG	3 cut(s)	420, 806, 1141
XhoI	CTCGAG	1 cut(s)	223
XmiI	GTMKAC	2 cut(s)	568, 1936
XmnI	GAANNNNTTC	2 cut(s)	2313, 2589
XspI	CTAG	8 cut(s)	77, 626, 969, 1587, 1742, 2267, 2829, 3038
ZrmI	AGTACT	3 cut(s)	2228, 3022, 3104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.