MD00G1110200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015616
Physical Location & Seq
Forward (+)
23067459 .. 23068130
672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1110200.v1.1.491

Sequence Viewer

Length: 672 bp
ATGTTGCAGCAGTACAACCACCAGCCACTGATGAAGATGGAGATCGCTGTGCTATCAGCCCAAGGCCTCAAAACCACATCGCCCTTTCTCTTCTCCAACAAAATCAGACCCTTCATCACCCTCACCACTCTCCCACGCACTATTGCTCCTCCATGCACACCCAAAACCAACGGTGATCACACCTTCCACGCGTACAAAACAAGGGTGGATGATCAAGGAGGCGTCAACCCTACATGGGGTGACAAATTTCATGTCCCTATTGATACAACTTTCTTCAACAACAGGTATTCTTGCATACATCTCCAGCTCTTCACCAAGAGGCTCATAATGGGCCGCGCTCAGCTGGGCTGGTGCCAAATCCCCGCCCACGATATTGGGTTCCCGCTGGCCGGTTCAGCTCGGTTCTTGAGTTACCGGCTACGGGCTAGAGACGGCTCCAGAACTAATGGAATTGTGAACCTTTCTGTGAAGTTGGAGAATTTACGTCCGGTCACCGATCACCGGCCTTTAAACTCAACGACTGCACCGGCTTTTGATATGTGTGGCACGGTCATTGGTACGCCGGTGACAACCCTACTGCCACCGGTGGGTGAGTGCTCTGTTAGTTGTCAAGGGCGGAGAGGTCAGATGGGTTTTGGTTGTAAAGAGGCAGATGACTGGGACCATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

224

Amino Acids

24.83

Weight (kDa)

9.32

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 124 2.4e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015616)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01760
malus_domestica MD00G1110200.v1.1 MD04G1064800.v1.1
prunus_persica Prupe.5G093100_v2.0.a1
pyrus_communis pycom04g05860 pycom06g07400
rosa_chinensis RchiOBHm_Chr7g0198961
rosa_laevigata RLG00000003829
rosa_multiflora Rmu_sc0006266.1_g000021
rosa_roxburghii Rroxscaffold_3G00257270
rosa_rugosa Rorug07G0051700
rosa_samantha Rh7AG174700 Rh7BG177900 Rh7CG183900 Rh7DG177300
rosa_wichuraiana Rw7G015320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AccII CGCG 2 cut(s) 191, 336
AciI CCGC 4 cut(s) 334, 363, 383, 616
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 2 cut(s) 245, 478
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 14, 194, 559
AfiI CCNNNNNNNGG 6 cut(s) 235, 236, 389, 421, 501, 587
AflIII ACRYGT 1 cut(s) 189
AgeI ACCGGT 1 cut(s) 583
AgsI TTSAA 1 cut(s) 277
AluBI AGCT 3 cut(s) 307, 343, 398
AluI AGCT 3 cut(s) 307, 343, 398
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 1 cut(s) 423
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 4 cut(s) 64, 331, 387, 503
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 2 cut(s) 245, 478
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AsiGI ACCGGT 1 cut(s) 583
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 2 cut(s) 331, 661
AsuHPI GGTGA 9 cut(s) 109, 115, 185, 251, 304, 484, 491, 577, 602
AvaII GGWCC 1 cut(s) 661
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BanI GGYRCC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 1 cut(s) 19
BccI CCATC 2 cut(s) 31, 622
BceAI ACGGC 1 cut(s) 448
BclI TGATCA 2 cut(s) 175, 211
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 1 cut(s) 426
BisI GCNGC 2 cut(s) 8, 334
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 2 cut(s) 9, 335
Bme18I GGWCC 1 cut(s) 661
BmgT120I GGNCC 2 cut(s) 331, 661
BmiI GGNNCC 4 cut(s) 353, 380, 436, 662
BmrI ACTGGG 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 667
BpmI CTGGAG 2 cut(s) 287, 421
Bpu1102I GCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 2 cut(s) 487, 583
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 6 cut(s) 235, 236, 389, 421, 501, 587
Bse118I RCCGGY 6 cut(s) 389, 414, 501, 526, 562, 583
Bse1I ACTGG 1 cut(s) 662
Bse8I GATNNNNATC 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 6 cut(s) 235, 236, 389, 421, 501, 587
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 662
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 19
BseYI CCCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 2 cut(s) 191, 336
BshFI GGCC 4 cut(s) 66, 333, 389, 505
BshNI GGYRCC 1 cut(s) 351
BshTI ACCGGT 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 599
BsiSI CCGG 7 cut(s) 390, 415, 488, 502, 527, 563, 584
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 6 cut(s) 235, 236, 389, 421, 501, 587
BsmAI GTCTC 1 cut(s) 423
BsmBI CGTCTC 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 4 cut(s) 66, 333, 389, 505
Bsp1286I GDGCHC 1 cut(s) 599
Bsp143I GATC 4 cut(s) 42, 175, 211, 496
Bsp1720I GCTNAGC 1 cut(s) 339
BspACI CCGC 4 cut(s) 334, 363, 383, 616
BspANI GGCC 4 cut(s) 66, 333, 389, 505
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 2 cut(s) 191, 336
BspLI GGNNCC 4 cut(s) 353, 380, 436, 662
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 351
BsrFI RCCGGY 6 cut(s) 389, 414, 501, 526, 562, 583
BsrI ACTGG 1 cut(s) 662
BssAI RCCGGY 6 cut(s) 389, 414, 501, 526, 562, 583
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 4 cut(s) 42, 175, 211, 496
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 61
Bst4CI ACNGT 2 cut(s) 173, 550
Bst6I CTCTTC 2 cut(s) 95, 314
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 339
BstEII GGTNACC 1 cut(s) 490
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 2 cut(s) 191, 336
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 4 cut(s) 45, 178, 214, 499
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 4 cut(s) 42, 175, 211, 496
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstPI GGTNACC 1 cut(s) 490
BstUI CGCG 2 cut(s) 191, 336
BstV1I GCAGC 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 374
BsuRI GGCC 4 cut(s) 66, 333, 389, 505
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 28
CfoI GCGC 1 cut(s) 338
Cfr10I RCCGGY 6 cut(s) 389, 414, 501, 526, 562, 583
Cfr13I GGNCC 2 cut(s) 331, 661
CseI GACGC 1 cut(s) 211
Csp6I GTAC 3 cut(s) 13, 193, 558
CspAI ACCGGT 1 cut(s) 583
CviAII CATG 4 cut(s) 153, 234, 251, 665
CviQI GTAC 3 cut(s) 13, 193, 558
DdeI CTNAG 1 cut(s) 339
DpnI GATC 4 cut(s) 44, 177, 213, 498
DpnII GATC 4 cut(s) 42, 175, 211, 496
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 2 cut(s) 95, 314
EarI CTCTTC 2 cut(s) 95, 314
EciI GGCGGA 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 61
Eco147I AGGCCT 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 661
Eco91I GGTNACC 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 61
Esp3I CGTCTC 1 cut(s) 423
FaeI CATG 4 cut(s) 156, 237, 254, 668
FaiI YATR 7 cut(s) 154, 235, 252, 296, 326, 539, 666
FaqI GGGAC 1 cut(s) 239
FatI CATG 4 cut(s) 152, 233, 250, 664
FauI CCCGC 2 cut(s) 370, 390
FbaI TGATCA 2 cut(s) 175, 211
Fnu4HI GCNGC 2 cut(s) 8, 334
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 2 cut(s) 8, 334
FspBI CTAG 1 cut(s) 426
GlaI GCGC 1 cut(s) 337
GluI GCNGC 2 cut(s) 8, 334
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 2 cut(s) 287, 421
HaeIII GGCC 4 cut(s) 66, 333, 389, 505
HapII CCGG 7 cut(s) 390, 415, 488, 502, 527, 563, 584
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 156, 237, 254, 668
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HpaII CCGG 7 cut(s) 390, 415, 488, 502, 527, 563, 584
HphI GGTGA 9 cut(s) 109, 115, 185, 251, 304, 484, 491, 577, 602
Hpy166II GTNNAC 2 cut(s) 226, 457
Hpy188I TCNGA 2 cut(s) 107, 627
Hpy188III TCNNGA 2 cut(s) 406, 438
Hpy8I GTNNAC 2 cut(s) 226, 457
HpyAV CCTTC 2 cut(s) 121, 193
HpyCH4III ACNGT 2 cut(s) 173, 550
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 4 cut(s) 7, 156, 294, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 1 cut(s) 484
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 156, 237, 254, 668
HspAI GCGC 1 cut(s) 336
Ksp22I TGATCA 2 cut(s) 175, 211
Kzo9I GATC 4 cut(s) 42, 175, 211, 496
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 2 cut(s) 151, 440
Lsp1109I GCAGC 1 cut(s) 19
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 4 cut(s) 239, 410, 490, 565
MalI GATC 4 cut(s) 44, 177, 213, 498
MboI GATC 4 cut(s) 42, 175, 211, 496
MboII GAAGA 4 cut(s) 46, 82, 265, 301
MhlI GDGCHC 1 cut(s) 599
MluCI AATT 3 cut(s) 245, 450, 478
MluI ACGCGT 1 cut(s) 189
MmeI TCCRAC 2 cut(s) 120, 453
MnlI CCTC 7 cut(s) 77, 131, 159, 212, 312, 614, 640
MseI TTAA 1 cut(s) 509
MspA1I CMGCKG 2 cut(s) 343, 385
MspI CCGG 7 cut(s) 390, 415, 488, 502, 527, 563, 584
MvnI CGCG 2 cut(s) 191, 336
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeII GATC 4 cut(s) 42, 175, 211, 496
NlaIII CATG 4 cut(s) 156, 237, 254, 668
NlaIV GGNNCC 4 cut(s) 353, 380, 436, 662
NmuCI GTSAC 3 cut(s) 239, 490, 565
PceI AGGCCT 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 583
PkrI GCNGC 2 cut(s) 9, 335
PspEI GGTNACC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 343
PspN4I GGNNCC 4 cut(s) 353, 380, 436, 662
PspPI GGNCC 2 cut(s) 331, 661
PstNI CAGNNNCTG 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 3 cut(s) 14, 194, 559
RsaNI GTAC 3 cut(s) 13, 193, 558
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 2 cut(s) 8, 334
Sau3AI GATC 4 cut(s) 42, 175, 211, 496
Sau96I GGNCC 2 cut(s) 331, 661
SduI GDGCHC 1 cut(s) 599
SetI ASST 8 cut(s) 185, 287, 309, 345, 400, 462, 487, 625
SgrAI CRCCGGYG 2 cut(s) 562, 583
SinI GGWCC 1 cut(s) 661
SmlI CTYRAG 1 cut(s) 406
SmoI CTYRAG 1 cut(s) 406
Sse9I AATT 3 cut(s) 245, 450, 478
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 4 cut(s) 334, 363, 383, 616
SspMI CTAG 1 cut(s) 426
StuI AGGCCT 1 cut(s) 66
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 2 cut(s) 173, 550
TaiI ACGT 1 cut(s) 487
TasI AATT 3 cut(s) 245, 450, 478
TatI WGTACW 1 cut(s) 12
TauI GCSGC 1 cut(s) 336
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 3 cut(s) 239, 490, 565
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 3 cut(s) 239, 490, 565
TspDTI ATGAA 3 cut(s) 47, 103, 239
TspRI CASTG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 661
XapI RAATTY 2 cut(s) 245, 478
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.