pycom04g05860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015616
Physical Location & Seq
Forward (+)
5721780 .. 5722463
684 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05860.1

Sequence Viewer

Length: 684 bp
ATGTTGAAGCAGCACAACCACCAGTCACTGATGAAGATGGAGATCACAGTGCTATCAGCCCAAGGTCTGAAGACCTCATCTCCCTCTCTATTCTCCAACAAAATCAGACCCTTCATCACCCTCACCACTCTTCAGCCCACTATTGCTCCGCCATGCACACCCAAAACCAACGGTGATCGCACCCTCCACGTGTACAAAACAAGGGTGGATGATCAAGGAGGCGTCAACCCTACGTGGGGAGACAAATTTCATGTCCCTATTGATACAACTTTCTTCAACAACGGGTATTCTTGCATATGTCTCCAGCTCTTCACCAAGAGGCTCATAATGGGCCGGGTTCAGCTGGGTTGGTGCCAAATCCCGGCCAATGATATTGGGTTCCCGCCGGCTAGTTCGGCACGGTTCTTGAGTTACCGGCTACGGGCTAGGGACGGTTCCAGGACTAATGGGATTGTGAACCTTTCTGTGAAGTTGGAGAATTTGGGTCCGGTCAACGATCACCAGCCTTTAAACTCAATGACTGCACCGGCTTTTGGTATGTGTGGCACAGTCATTGGTACGCCGGTCACAACCCTACTGCCACCCTTGGGTGAGTGCTCTGTGAGTCGGAGAGGTCGGAGAGGTCACATGGGTTTTGGTTGTAAAGAGGCAGATGGCTGGGACCATGTCAATAATTTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

228

Amino Acids

25.0

Weight (kDa)

9.56

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 124 6.4e-07 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015616)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01760
malus_domestica MD00G1110200.v1.1 MD04G1064800.v1.1
prunus_persica Prupe.5G093100_v2.0.a1
pyrus_communis pycom04g05860 pycom06g07400
rosa_chinensis RchiOBHm_Chr7g0198961
rosa_laevigata RLG00000003829
rosa_multiflora Rmu_sc0006266.1_g000021
rosa_roxburghii Rroxscaffold_3G00257270
rosa_rugosa Rorug07G0051700
rosa_samantha Rh7AG174700 Rh7BG177900 Rh7CG183900 Rh7DG177300
rosa_wichuraiana Rw7G015320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AciI CCGC 2 cut(s) 149, 383
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 2 cut(s) 245, 478
AcuI CTGAAG 2 cut(s) 89, 116
AcvI CACGTG 1 cut(s) 190
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 194, 559
AfiI CCNNNNNNNGG 6 cut(s) 235, 236, 361, 421, 533, 587
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 2 cut(s) 7, 277
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 2 cut(s) 307, 343
AluI AGCT 2 cut(s) 307, 343
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 2 cut(s) 234, 305
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 2 cut(s) 331, 363
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 245, 478
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AspS9I GGNCC 3 cut(s) 331, 485, 661
AsuC2I CCSGG 2 cut(s) 335, 362
AsuHPI GGTGA 6 cut(s) 109, 115, 185, 304, 491, 602
AvaII GGWCC 2 cut(s) 485, 661
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BanI GGYRCC 1 cut(s) 351
BbrPI CACGTG 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 1 cut(s) 22
BccI CCATC 2 cut(s) 31, 647
BciT130I CCWGG 1 cut(s) 439
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 335, 362
BcoDI GTCTC 2 cut(s) 234, 305
BfaI CTAG 2 cut(s) 390, 426
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 3 cut(s) 335, 362, 439
Bme18I GGWCC 2 cut(s) 485, 661
BmgT120I GGNCC 3 cut(s) 331, 485, 661
BmiI GGNNCC 5 cut(s) 353, 380, 436, 486, 662
BmrFI CCNGG 3 cut(s) 335, 362, 439
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 427
BpuMI CCSGG 2 cut(s) 335, 362
BsaAI YACGTR 2 cut(s) 190, 234
BsaBI GATNNNNATC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 61, 585
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 130, 160
Bsc4I CCNNNNNNNGG 6 cut(s) 235, 236, 361, 421, 533, 587
Bse118I RCCGGY 4 cut(s) 385, 414, 526, 562
Bse1I ACTGG 1 cut(s) 22
Bse8I GATNNNNATC 1 cut(s) 41
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 61, 585
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 6 cut(s) 235, 236, 361, 421, 533, 587
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 22
BseYI CCCAGC 2 cut(s) 343, 657
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 2 cut(s) 333, 365
BshNI GGYRCC 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 599
BsiSI CCGG 7 cut(s) 334, 362, 386, 415, 488, 527, 563
BslFI GGGAC 3 cut(s) 239, 443, 674
BslI CCNNNNNNNGG 6 cut(s) 235, 236, 361, 421, 533, 587
BsmAI GTCTC 2 cut(s) 234, 305
BsmFI GGGAC 3 cut(s) 239, 443, 674
BsnI GGCC 2 cut(s) 333, 365
Bsp1286I GDGCHC 1 cut(s) 599
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 4 cut(s) 42, 175, 211, 496
BspACI CCGC 2 cut(s) 149, 383
BspANI GGCC 2 cut(s) 333, 365
BspLI GGNNCC 5 cut(s) 353, 380, 436, 486, 662
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 351
BsrFI RCCGGY 4 cut(s) 385, 414, 526, 562
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 1 cut(s) 22
BssAI RCCGGY 4 cut(s) 385, 414, 526, 562
BssECI CCNNGG 2 cut(s) 61, 585
BssMI GATC 4 cut(s) 42, 175, 211, 496
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 61, 585
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 5 cut(s) 49, 173, 402, 434, 550
Bst6I CTCTTC 2 cut(s) 135, 314
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 192
BstBAI YACGTR 2 cut(s) 190, 234
BstC8I GCNNGC 1 cut(s) 387
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 4 cut(s) 45, 178, 214, 499
BstMAI GTCTC 2 cut(s) 234, 305
BstMBI GATC 4 cut(s) 42, 175, 211, 496
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 439
BstSCI CCNGG 3 cut(s) 333, 360, 437
BstV1I GCAGC 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 77
BsuRI GGCC 2 cut(s) 333, 365
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 26, 54
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 28
Cfr10I RCCGGY 4 cut(s) 385, 414, 526, 562
Cfr13I GGNCC 3 cut(s) 331, 485, 661
CseI GACGC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 193, 558
CviAII CATG 4 cut(s) 153, 251, 628, 665
CviQI GTAC 2 cut(s) 193, 558
DpnI GATC 4 cut(s) 44, 177, 213, 498
DpnII GATC 4 cut(s) 42, 175, 211, 496
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 2 cut(s) 135, 314
EarI CTCTTC 2 cut(s) 135, 314
EciI GGCGGA 1 cut(s) 138
Eco130I CCWWGG 2 cut(s) 61, 585
Eco47I GGWCC 2 cut(s) 485, 661
Eco57I CTGAAG 2 cut(s) 89, 116
Eco72I CACGTG 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 437
EcoT14I CCWWGG 2 cut(s) 61, 585
ErhI CCWWGG 2 cut(s) 61, 585
FaeI CATG 4 cut(s) 156, 254, 631, 668
FaiI YATR 8 cut(s) 154, 252, 296, 298, 326, 539, 629, 666
FaqI GGGAC 3 cut(s) 239, 443, 674
FatI CATG 4 cut(s) 152, 250, 627, 664
FauI CCCGC 1 cut(s) 390
FauNDI CATATG 1 cut(s) 296
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 1 cut(s) 11
FspBI CTAG 2 cut(s) 390, 426
GluI GCNGC 1 cut(s) 11
GsaI CCCAGC 2 cut(s) 347, 661
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 333, 365
HapII CCGG 7 cut(s) 334, 362, 386, 415, 488, 527, 563
HgaI GACGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 156, 254, 631, 668
HincII GTYRAC 2 cut(s) 226, 493
HindII GTYRAC 2 cut(s) 226, 493
HinfI GANTC 1 cut(s) 604
HpaII CCGG 7 cut(s) 334, 362, 386, 415, 488, 527, 563
HphI GGTGA 6 cut(s) 109, 115, 185, 304, 491, 602
Hpy166II GTNNAC 4 cut(s) 193, 226, 457, 493
Hpy188I TCNGA 4 cut(s) 69, 107, 609, 618
Hpy188III TCNNGA 1 cut(s) 406
Hpy8I GTNNAC 4 cut(s) 193, 226, 457, 493
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 5 cut(s) 49, 173, 402, 434, 550
HpyCH4IV ACGT 2 cut(s) 189, 233
HpyCH4V TGCA 4 cut(s) 156, 294, 524, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 189, 233
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 156, 254, 631, 668
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 4 cut(s) 42, 175, 211, 496
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 1 cut(s) 22
MaeI CTAG 2 cut(s) 390, 426
MaeII ACGT 2 cut(s) 189, 233
MaeIII GTNAC 4 cut(s) 24, 410, 565, 623
MalI GATC 4 cut(s) 44, 177, 213, 498
MboI GATC 4 cut(s) 42, 175, 211, 496
MboII GAAGA 5 cut(s) 46, 82, 122, 265, 301
MhlI GDGCHC 1 cut(s) 599
MluCI AATT 3 cut(s) 245, 478, 673
MlyI GAGTC 1 cut(s) 613
MmeI TCCRAC 4 cut(s) 120, 453, 587, 596
MnlI CCTC 9 cut(s) 85, 94, 131, 194, 212, 312, 605, 614, 640
MroNI GCCGGC 1 cut(s) 385
MseI TTAA 1 cut(s) 509
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 7 cut(s) 334, 362, 386, 415, 488, 527, 563
MspR9I CCNGG 3 cut(s) 335, 362, 439
MvaI CCWGG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 395
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 2 cut(s) 335, 362
NdeI CATATG 1 cut(s) 296
NdeII GATC 4 cut(s) 42, 175, 211, 496
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 4 cut(s) 156, 254, 631, 668
NlaIV GGNNCC 5 cut(s) 353, 380, 436, 486, 662
NmuCI GTSAC 3 cut(s) 24, 565, 623
PciSI GCTCTTC 1 cut(s) 314
PdiI GCCGGC 1 cut(s) 387
PflFI GACNNNGTC 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 437
PkrI GCNGC 1 cut(s) 12
PleI GAGTC 1 cut(s) 612
PmaCI CACGTG 1 cut(s) 190
PmlI CACGTG 1 cut(s) 190
PpsI GAGTC 1 cut(s) 612
Ppu21I YACGTR 2 cut(s) 190, 234
Psp6I CCWGG 1 cut(s) 437
PspCI CACGTG 1 cut(s) 190
PspFI CCCAGC 2 cut(s) 343, 657
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 5 cut(s) 353, 380, 436, 486, 662
PspPI GGNCC 3 cut(s) 331, 485, 661
PstNI CAGNNNCTG 1 cut(s) 28
PsyI GACNNNGTC 1 cut(s) 665
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 2 cut(s) 194, 559
RsaNI GTAC 2 cut(s) 193, 558
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 4 cut(s) 42, 175, 211, 496
Sau96I GGNCC 3 cut(s) 331, 485, 661
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 3 cut(s) 335, 362, 439
SduI GDGCHC 1 cut(s) 599
SetI ASST 9 cut(s) 67, 77, 192, 236, 309, 345, 462, 616, 625
SinI GGWCC 2 cut(s) 485, 661
SmlI CTYRAG 1 cut(s) 406
SmoI CTYRAG 1 cut(s) 406
Sse9I AATT 3 cut(s) 245, 478, 673
SsiI CCGC 2 cut(s) 149, 383
SspMI CTAG 2 cut(s) 390, 426
StyD4I CCNGG 3 cut(s) 333, 360, 437
StyI CCWWGG 2 cut(s) 61, 585
TaaI ACNGT 5 cut(s) 49, 173, 402, 434, 550
TaiI ACGT 2 cut(s) 192, 236
TasI AATT 3 cut(s) 245, 478, 673
TatI WGTACW 1 cut(s) 192
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TscAI CASTG 2 cut(s) 33, 54
TseFI GTSAC 3 cut(s) 24, 565, 623
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 3 cut(s) 24, 565, 623
TspDTI ATGAA 3 cut(s) 47, 103, 239
TspRI CASTG 2 cut(s) 33, 54
Tth111I GACNNNGTC 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 485, 661
XapI RAATTY 2 cut(s) 245, 478
XspI CTAG 2 cut(s) 390, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.