Rroxscaffold_3G00257270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015616
Physical Location & Seq
Reverse (-)
51652882 .. 51653508
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257270.1

Sequence Viewer

Length: 627 bp
ATGGAGATCACAGTGATATCAGCTCAAGGCCTCAAGAACTCTTCTCGCTTGTCCCACAAGATTAGACCCTTTATCACCCTCACCACTTCTCCACCGGCTGCCACAACATGCCGGTCCTCCGGCGACGAGAAGTGCCACCTGTACAAAACGAGGGTGGATGATGAAGGTGGGATCAACCCAACATGGGGTGACAAGTTCTTGGTTCCTCTGGATGCTAGTTTCTTCAGCAACAGGTATTCTTGCATATATCTCCAGCTTTACACTAAGCGGCTCATAATGGGCCGGGCGCAGCTGGGCTGGTGTCAAATCCCGGCCTATGATATTGTCGGGTTCCCGGCGACGGGCTCGGTCCGGTACCTGAGTTACCGGCTACGGGCTAGGGACGGTACGAGGACTCATGGGGTTGTGAACGTGGCTATCAAGTTGGAGAATTTGGCGCCGGTGAGCAATCAGAGGGTGGTGTCAGTAAACTCTATCTCATCGACATTTGACACGTGTGGCACGGTTATTGGTATGCCGGTGGCACAGCTATCACCTTTGGCGGGTGAGTGCTCCGTTCCGTGTCAGGGGCAGATAAGTCACTTTGGTTTTGGTTGTAAAGAGGGAAAAAGATGGAGCCATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

208

Amino Acids

22.82

Weight (kDa)

9.3

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 105 1.9e-07 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015616)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01760
malus_domestica MD00G1110200.v1.1 MD04G1064800.v1.1
prunus_persica Prupe.5G093100_v2.0.a1
pyrus_communis pycom04g05860 pycom06g07400
rosa_chinensis RchiOBHm_Chr7g0198961
rosa_laevigata RLG00000003829
rosa_multiflora Rmu_sc0006266.1_g000021
rosa_roxburghii Rroxscaffold_3G00257270
rosa_rugosa Rorug07G0051700
rosa_samantha Rh7AG174700 Rh7BG177900 Rh7CG183900 Rh7DG177300
rosa_wichuraiana Rw7G015320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 354, 436
AciI CCGC 2 cut(s) 268, 542
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 208
AcvI CACGTG 1 cut(s) 495
AcyI GRCGYC 1 cut(s) 437
AfaI GTAC 3 cut(s) 143, 356, 388
AfiI CCNNNNNNNGG 7 cut(s) 184, 185, 340, 341, 373, 542, 566
AflIII ACRYGT 2 cut(s) 492, 494
AluBI AGCT 4 cut(s) 23, 256, 292, 529
AluI AGCT 4 cut(s) 23, 256, 292, 529
Alw21I GWGCWC 1 cut(s) 554
AlwI GGATC 1 cut(s) 179
AoxI GGCC 3 cut(s) 28, 280, 312
ApeKI GCWGC 2 cut(s) 98, 289
ApoI RAATTY 1 cut(s) 430
Asp718I GGTACC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 289, 439
AspS9I GGNCC 3 cut(s) 114, 280, 349
AsuC2I CCSGG 3 cut(s) 284, 311, 335
AsuHPI GGTGA 6 cut(s) 67, 73, 200, 454, 525, 557
AvaII GGWCC 2 cut(s) 114, 349
BaeI ACNNNNGTAYC 2 cut(s) 346, 379
BanI GGYRCC 2 cut(s) 354, 436
BanII GRGCYC 1 cut(s) 347
BbrPI CACGTG 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 85, 301
BccI CCATC 1 cut(s) 606
BcnI CCSGG 3 cut(s) 284, 311, 335
BfaI CTAG 2 cut(s) 216, 378
BfoI RGCGCY 1 cut(s) 440
BisI GCNGC 3 cut(s) 99, 269, 290
BlsI GCNGC 3 cut(s) 100, 270, 291
Bme1390I CCNGG 3 cut(s) 284, 311, 335
Bme18I GGWCC 2 cut(s) 114, 349
BmgT120I GGNCC 3 cut(s) 114, 280, 349
BmiI GGNNCC 5 cut(s) 204, 332, 356, 438, 617
BmrFI CCNGG 3 cut(s) 284, 311, 335
BmsI GCATC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 236
BpuEI CTTGAG 2 cut(s) 9, 17
BpuMI CCSGG 3 cut(s) 284, 311, 335
BsaAI YACGTR 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 437
BsaWI WCCGGW 1 cut(s) 351
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 7 cut(s) 184, 185, 340, 341, 373, 542, 566
Bse118I RCCGGY 5 cut(s) 94, 111, 366, 439, 517
BseGI GGATG 2 cut(s) 163, 217
BseLI CCNNNNNNNGG 7 cut(s) 184, 185, 340, 341, 373, 542, 566
BseMII CTCAG 1 cut(s) 350
BseXI GCAGC 2 cut(s) 85, 301
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 3 cut(s) 30, 282, 314
BshNI GGYRCC 2 cut(s) 354, 436
BsiHKAI GWGCWC 1 cut(s) 554
BslFI GGGAC 2 cut(s) 37, 395
BslI CCNNNNNNNGG 7 cut(s) 184, 185, 340, 341, 373, 542, 566
BsmFI GGGAC 2 cut(s) 37, 395
BsnI GGCC 3 cut(s) 30, 282, 314
Bsp1286I GDGCHC 2 cut(s) 347, 554
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 2 cut(s) 6, 171
BspACI CCGC 2 cut(s) 268, 542
BspANI GGCC 3 cut(s) 30, 282, 314
BspCNI CTCAG 1 cut(s) 351
BspLI GGNNCC 5 cut(s) 204, 332, 356, 438, 617
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 2 cut(s) 354, 436
BsrFI RCCGGY 5 cut(s) 94, 111, 366, 439, 517
BsrGI TGTACA 1 cut(s) 141
BssAI RCCGGY 5 cut(s) 94, 111, 366, 439, 517
BssMI GATC 2 cut(s) 6, 171
BssNI GRCGYC 1 cut(s) 437
Bst4CI ACNGT 3 cut(s) 13, 386, 505
Bst6I CTCTTC 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 495
BstDEI CTNAG 2 cut(s) 264, 359
BstF5I GGATG 2 cut(s) 163, 217
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 2 cut(s) 289, 439
BstKTI GATC 2 cut(s) 9, 174
BstMBI GATC 2 cut(s) 6, 171
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 282, 309, 333
BstV1I GCAGC 2 cut(s) 85, 301
BsuRI GGCC 3 cut(s) 30, 282, 314
BtsCI GGATG 2 cut(s) 163, 217
BtsIMutI CAGTG 1 cut(s) 18
CfoI GCGC 2 cut(s) 289, 439
Cfr10I RCCGGY 5 cut(s) 94, 111, 366, 439, 517
Cfr13I GGNCC 3 cut(s) 114, 280, 349
CpoI CGGWCCG 1 cut(s) 349
Csp6I GTAC 3 cut(s) 142, 355, 387
CspI CGGWCCG 1 cut(s) 349
CviAII CATG 4 cut(s) 108, 183, 398, 620
CviQI GTAC 3 cut(s) 142, 355, 387
DdeI CTNAG 2 cut(s) 264, 359
DinI GGCGCC 1 cut(s) 438
DpnI GATC 2 cut(s) 8, 173
DpnII GATC 2 cut(s) 6, 171
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 347
Eco32I GATATC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 114, 349
Eco57I CTGAAG 1 cut(s) 208
Eco72I CACGTG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 347
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
FaeI CATG 4 cut(s) 111, 186, 401, 623
FaiI YATR 9 cut(s) 109, 184, 245, 247, 275, 318, 399, 515, 621
FaqI GGGAC 2 cut(s) 37, 395
FatI CATG 4 cut(s) 107, 182, 397, 619
FauI CCCGC 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 99, 269, 290
FokI GGATG 2 cut(s) 170, 224
FriOI GRGCYC 1 cut(s) 347
Fsp4HI GCNGC 3 cut(s) 99, 269, 290
FspBI CTAG 2 cut(s) 216, 378
GlaI GCGC 2 cut(s) 288, 438
GluI GCNGC 3 cut(s) 99, 269, 290
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 236
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 3 cut(s) 30, 282, 314
HhaI GCGC 2 cut(s) 289, 439
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 4 cut(s) 111, 186, 401, 623
Hin6I GCGC 2 cut(s) 287, 437
HinP1I GCGC 2 cut(s) 287, 437
HinfI GANTC 1 cut(s) 394
HphI GGTGA 6 cut(s) 67, 73, 200, 454, 525, 557
Hpy166II GTNNAC 2 cut(s) 409, 469
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 2 cut(s) 34, 209
Hpy8I GTNNAC 2 cut(s) 409, 469
Hpy99I CGWCG 2 cut(s) 128, 343
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 3 cut(s) 13, 386, 505
HpyCH4IV ACGT 2 cut(s) 411, 494
HpyCH4V TGCA 1 cut(s) 243
HpyF3I CTNAG 2 cut(s) 264, 359
HpySE526I ACGT 2 cut(s) 411, 494
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 4 cut(s) 111, 186, 401, 623
HspAI GCGC 2 cut(s) 287, 437
KasI GGCGCC 1 cut(s) 436
KpnI GGTACC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 6, 171
LmnI GCTCC 2 cut(s) 557, 615
Lsp1109I GCAGC 2 cut(s) 85, 301
LweI GCATC 1 cut(s) 202
MaeI CTAG 2 cut(s) 216, 378
MaeII ACGT 2 cut(s) 411, 494
MaeIII GTNAC 3 cut(s) 188, 362, 578
MalI GATC 2 cut(s) 8, 173
MboI GATC 2 cut(s) 6, 171
MboII GAAGA 2 cut(s) 33, 214
MhlI GDGCHC 2 cut(s) 347, 554
MluCI AATT 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 8 cut(s) 41, 89, 127, 144, 216, 384, 447, 595
MspA1I CMGCKG 1 cut(s) 292
MspR9I CCNGG 3 cut(s) 284, 311, 335
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 3 cut(s) 284, 311, 335
NdeII GATC 2 cut(s) 6, 171
NlaIII CATG 4 cut(s) 111, 186, 401, 623
NlaIV GGNNCC 5 cut(s) 204, 332, 356, 438, 617
NmuCI GTSAC 2 cut(s) 188, 578
NspI RCATGY 1 cut(s) 111
PceI AGGCCT 1 cut(s) 30
PkrI GCNGC 3 cut(s) 100, 270, 291
PleI GAGTC 1 cut(s) 388
PluTI GGCGCC 1 cut(s) 440
PmaCI CACGTG 1 cut(s) 495
PmlI CACGTG 1 cut(s) 495
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 1 cut(s) 495
PspCI CACGTG 1 cut(s) 495
PspFI CCCAGC 1 cut(s) 292
PspN4I GGNNCC 5 cut(s) 204, 332, 356, 438, 617
PspPI GGNCC 3 cut(s) 114, 280, 349
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 3 cut(s) 143, 356, 388
RsaNI GTAC 3 cut(s) 142, 355, 387
Rsr2I CGGWCCG 1 cut(s) 349
RsrII CGGWCCG 1 cut(s) 349
SatI GCNGC 3 cut(s) 99, 269, 290
Sau3AI GATC 2 cut(s) 6, 171
Sau96I GGNCC 3 cut(s) 114, 280, 349
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 3 cut(s) 284, 311, 335
SduI GDGCHC 2 cut(s) 347, 554
SfaNI GCATC 1 cut(s) 202
SfoI GGCGCC 1 cut(s) 438
SgrAI CRCCGGYG 1 cut(s) 439
SinI GGWCC 2 cut(s) 114, 349
SmlI CTYRAG 2 cut(s) 24, 32
SmoI CTYRAG 2 cut(s) 24, 32
Sse9I AATT 1 cut(s) 430
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 2 cut(s) 268, 542
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 2 cut(s) 216, 378
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 282, 309, 333
TaaI ACNGT 3 cut(s) 13, 386, 505
TaiI ACGT 2 cut(s) 414, 497
TaqI TCGA 1 cut(s) 482
TaqII GACCGA 1 cut(s) 337
TasI AATT 1 cut(s) 430
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 271
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 188, 578
TseI GCWGC 2 cut(s) 98, 289
Tsp45I GTSAC 2 cut(s) 188, 578
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 2 cut(s) 544, 549
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 114, 349
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 111
XspI CTAG 2 cut(s) 216, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.