MD04G1064800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015616
Physical Location & Seq
Forward (+)
8714058 .. 8714648
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1064800.v1.1.491

Sequence Viewer

Length: 591 bp
ATGTTGAAGCAGCACAGCCACCAGCCACTGATGAAGATGGAGATCACAGTGCTATCAGCCCAAGGTCTGAAGACCTCATCTCCCTCTCTCTTCTCCAACAAAATCAGACCCTTCGTCACCATCACCACTCTCCCGTCCACTATTGCTCAGCCATGCACACCCAAAACCAACGGTGATCGCACCCTCCACGTGTACAAAACAAGGGTGGATGATCAAGGAGGCGTCAGCCCTACATGGGGTGACAAATTTCATGTCCCTATTGATACAACTTTCTTCAACAACAGGTATTCTTGCATATATCTCCAGCTCTTCACCAAGAGGCTCATAATGGGCCGGGTTGAGCTGGGTTGGTGCCAAATCCCGGTCAATGATATTGGGTTCCCGCCGGCCAGTTCGGCACGGTTCTTGAGTTACCGGCTACGGGCTAGGGACGGTTCCAGGACTAATGGGATTGTGAACCTTTCTGTGAAGTTGGAGAATTTGGGTCCGGTCACCGATCACCGGCCTTTAAACTCAACGACTGTACCGGATTTTGGTATGTGTGGCAGTCATTGGTACGCCGGTCACAACCCTACTGCCACCATTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

197

Amino Acids

21.82

Weight (kDa)

9.69

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 124 6.7e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015616)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01760
malus_domestica MD00G1110200.v1.1 MD04G1064800.v1.1
prunus_persica Prupe.5G093100_v2.0.a1
pyrus_communis pycom04g05860 pycom06g07400
rosa_chinensis RchiOBHm_Chr7g0198961
rosa_laevigata RLG00000003829
rosa_multiflora Rmu_sc0006266.1_g000021
rosa_roxburghii Rroxscaffold_3G00257270
rosa_rugosa Rorug07G0051700
rosa_samantha Rh7AG174700 Rh7BG177900 Rh7CG183900 Rh7DG177300
rosa_wichuraiana Rw7G015320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AciI CCGC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 2 cut(s) 245, 478
AcuI CTGAAG 1 cut(s) 89
AcvI CACGTG 1 cut(s) 190
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 194, 525, 557
AfiI CCNNNNNNNGG 7 cut(s) 235, 236, 361, 421, 501, 533, 585
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 2 cut(s) 7, 277
AhdI GACNNNNNGTC 1 cut(s) 113
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 2 cut(s) 307, 343
AluI AGCT 2 cut(s) 307, 343
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 3 cut(s) 331, 387, 503
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 245, 478
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AspS9I GGNCC 2 cut(s) 331, 485
AsuC2I CCSGG 2 cut(s) 335, 362
AsuHPI GGTGA 7 cut(s) 109, 115, 185, 251, 304, 484, 491
AvaII GGWCC 1 cut(s) 485
BaeI ACNNNNGTAYC 4 cut(s) 507, 540, 547, 580
BanI GGYRCC 1 cut(s) 351
BbrPI CACGTG 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 22
BccI CCATC 2 cut(s) 31, 128
BciT130I CCWGG 1 cut(s) 439
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 335, 362
BfaI CTAG 1 cut(s) 426
BglI GCCNNNNNGGC 1 cut(s) 395
BisI GCNGC 1 cut(s) 11
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 3 cut(s) 335, 362, 439
Bme18I GGWCC 1 cut(s) 485
BmeRI GACNNNNNGTC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 331, 485
BmiI GGNNCC 4 cut(s) 353, 380, 436, 486
BmrFI CCNGG 3 cut(s) 335, 362, 439
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 427
BpuMI CCSGG 2 cut(s) 335, 362
BsaAI YACGTR 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 2 cut(s) 487, 526
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 7 cut(s) 235, 236, 361, 421, 501, 533, 585
Bse118I RCCGGY 4 cut(s) 385, 414, 501, 560
Bse1I ACTGG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 41
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 7 cut(s) 235, 236, 361, 421, 501, 533, 585
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 22
BseYI CCCAGC 1 cut(s) 343
BshFI GGCC 3 cut(s) 333, 389, 505
BshNI GGYRCC 1 cut(s) 351
BsiSI CCGG 8 cut(s) 334, 362, 386, 415, 488, 502, 527, 561
BslFI GGGAC 2 cut(s) 239, 443
BslI CCNNNNNNNGG 7 cut(s) 235, 236, 361, 421, 501, 533, 585
BsmFI GGGAC 2 cut(s) 239, 443
BsnI GGCC 3 cut(s) 333, 389, 505
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 4 cut(s) 42, 175, 211, 496
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 1 cut(s) 383
BspANI GGCC 3 cut(s) 333, 389, 505
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 4 cut(s) 353, 380, 436, 486
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 351
BsrFI RCCGGY 4 cut(s) 385, 414, 501, 560
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 1 cut(s) 390
BssAI RCCGGY 4 cut(s) 385, 414, 501, 560
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 4 cut(s) 42, 175, 211, 496
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 5 cut(s) 49, 173, 402, 434, 523
Bst6I CTCTTC 2 cut(s) 95, 314
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 147
BstEII GGTNACC 1 cut(s) 490
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 4 cut(s) 45, 178, 214, 499
BstMBI GATC 4 cut(s) 42, 175, 211, 496
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 439
BstPI GGTNACC 1 cut(s) 490
BstSCI CCNGG 3 cut(s) 333, 360, 437
BstV1I GCAGC 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 77
BsuRI GGCC 3 cut(s) 333, 389, 505
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 26, 54
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 28
Cfr10I RCCGGY 4 cut(s) 385, 414, 501, 560
Cfr13I GGNCC 2 cut(s) 331, 485
CseI GACGC 1 cut(s) 211
Csp6I GTAC 3 cut(s) 193, 524, 556
CviAII CATG 3 cut(s) 153, 234, 251
CviQI GTAC 3 cut(s) 193, 524, 556
DdeI CTNAG 1 cut(s) 147
DpnI GATC 4 cut(s) 44, 177, 213, 498
DpnII GATC 4 cut(s) 42, 175, 211, 496
DraI TTTAAA 1 cut(s) 510
DriI GACNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 2 cut(s) 95, 314
Eam1105I GACNNNNNGTC 1 cut(s) 113
EarI CTCTTC 2 cut(s) 95, 314
Eco130I CCWWGG 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 485
Eco57I CTGAAG 1 cut(s) 89
Eco72I CACGTG 1 cut(s) 190
Eco91I GGTNACC 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 437
EcoT14I CCWWGG 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 61
FaeI CATG 3 cut(s) 156, 237, 254
FaiI YATR 7 cut(s) 154, 235, 252, 296, 298, 326, 539
FaqI GGGAC 2 cut(s) 239, 443
FatI CATG 3 cut(s) 152, 233, 250
FauI CCCGC 1 cut(s) 390
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 1 cut(s) 11
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 11
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 3 cut(s) 333, 389, 505
HapII CCGG 8 cut(s) 334, 362, 386, 415, 488, 502, 527, 561
HgaI GACGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 156, 237, 254
HpaII CCGG 8 cut(s) 334, 362, 386, 415, 488, 502, 527, 561
HphI GGTGA 7 cut(s) 109, 115, 185, 251, 304, 484, 491
Hpy166II GTNNAC 3 cut(s) 138, 193, 457
Hpy188I TCNGA 2 cut(s) 69, 107
Hpy188III TCNNGA 1 cut(s) 406
Hpy8I GTNNAC 3 cut(s) 138, 193, 457
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 5 cut(s) 49, 173, 402, 434, 523
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 156, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 156, 237, 254
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 4 cut(s) 42, 175, 211, 496
LguI GCTCTTC 1 cut(s) 314
Lsp1109I GCAGC 1 cut(s) 22
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 5 cut(s) 115, 239, 410, 490, 563
MalI GATC 4 cut(s) 44, 177, 213, 498
MboI GATC 4 cut(s) 42, 175, 211, 496
MboII GAAGA 5 cut(s) 46, 82, 82, 265, 301
MluCI AATT 2 cut(s) 245, 478
MmeI TCCRAC 2 cut(s) 120, 453
MnlI CCTC 5 cut(s) 85, 94, 194, 212, 312
MroNI GCCGGC 1 cut(s) 385
MseI TTAA 1 cut(s) 509
MspI CCGG 8 cut(s) 334, 362, 386, 415, 488, 502, 527, 561
MspR9I CCNGG 3 cut(s) 335, 362, 439
MvaI CCWGG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 395
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 2 cut(s) 335, 362
NdeII GATC 4 cut(s) 42, 175, 211, 496
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 3 cut(s) 156, 237, 254
NlaIV GGNNCC 4 cut(s) 353, 380, 436, 486
NmuCI GTSAC 4 cut(s) 115, 239, 490, 563
PciSI GCTCTTC 1 cut(s) 314
PdiI GCCGGC 1 cut(s) 387
PfoI TCCNGGA 1 cut(s) 437
PkrI GCNGC 1 cut(s) 12
PmaCI CACGTG 1 cut(s) 190
PmlI CACGTG 1 cut(s) 190
Ppu21I YACGTR 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 437
PspCI CACGTG 1 cut(s) 190
PspEI GGTNACC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 4 cut(s) 353, 380, 436, 486
PspPI GGNCC 2 cut(s) 331, 485
PstNI CAGNNNCTG 1 cut(s) 28
RsaI GTAC 3 cut(s) 194, 525, 557
RsaNI GTAC 3 cut(s) 193, 524, 556
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 4 cut(s) 42, 175, 211, 496
Sau96I GGNCC 2 cut(s) 331, 485
ScrFI CCNGG 3 cut(s) 335, 362, 439
SetI ASST 7 cut(s) 67, 77, 192, 287, 309, 345, 462
SinI GGWCC 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 406
SmoI CTYRAG 1 cut(s) 406
Sse9I AATT 2 cut(s) 245, 478
SsiI CCGC 1 cut(s) 383
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 3 cut(s) 333, 360, 437
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 5 cut(s) 49, 173, 402, 434, 523
TaiI ACGT 1 cut(s) 192
TasI AATT 2 cut(s) 245, 478
TatI WGTACW 1 cut(s) 192
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TscAI CASTG 2 cut(s) 33, 54
TseFI GTSAC 4 cut(s) 115, 239, 490, 563
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 4 cut(s) 115, 239, 490, 563
TspDTI ATGAA 2 cut(s) 47, 239
TspRI CASTG 2 cut(s) 33, 54
VpaK11BI GGWCC 1 cut(s) 485
XapI RAATTY 2 cut(s) 245, 478
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.