RLG00000003829

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015616
Physical Location & Seq
Reverse (-)
53231397 .. 53232023
627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003829

Sequence Viewer

Length: 627 bp
ATGGAGATCACAGTGATATCAGCTCAAGGCCTCAAGAACTCTTCTCACTTTTCCCAGAAGATTAGACCCTTTATCACCCTCACAACTACTCCACCGGCTGCCACACCATGCCGGTCCTCGGGCGACGAGAAGTGCCACGTGTACAAAACGAGGGTGGATGATGAAGGTGGGATCAACCCAACATGGGGTGACAAGTTCTTGGTTCCTCTGGATGCCAGTTTCTTCAGCAACAGGTATTCTTGCATATATCTCCAGCTTTACACTAAGCGGCTCATAATGGGCCGGGCTCAGCTGGGCTGGTGTCAAATCCCGGCCTATGATATTGTCGGGTTCCCGGCGACGGGCTCGGTCCGGTACCTGAGTTACCGGCTACGGGCTAGGGACGGTACGAGAACTCATGGGGTTGTGAACGTGGCTATTAAGTTGGAGAATTTGGCGCCGGTGAGCAATCAGAGGGTGGTGTCAGTAAACTCTATCACATCGACATTTGACACGTGTGGCACGGTTATTGGTACGCCGGTGGCACAGCTATCACCTTTGGCGGGTGAGTGCTCCGTTACGTGTCAGGGGCAGATAAGTCACTTTGGTTTTGGTTGTAAAGAGGGAAATAGATGGAGCCATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

209

Amino Acids

22.8

Weight (kDa)

9.08

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 105 3.3e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015616)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01760
malus_domestica MD00G1110200.v1.1 MD04G1064800.v1.1
prunus_persica Prupe.5G093100_v2.0.a1
pyrus_communis pycom04g05860 pycom06g07400
rosa_chinensis RchiOBHm_Chr7g0198961
rosa_laevigata RLG00000003829
rosa_multiflora Rmu_sc0006266.1_g000021
rosa_roxburghii Rroxscaffold_3G00257270
rosa_rugosa Rorug07G0051700
rosa_samantha Rh7AG174700 Rh7BG177900 Rh7CG183900 Rh7DG177300
rosa_wichuraiana Rw7G015320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 354, 436
AciI CCGC 2 cut(s) 268, 542
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 208
AcvI CACGTG 2 cut(s) 139, 495
AcyI GRCGYC 1 cut(s) 437
AfaI GTAC 4 cut(s) 143, 356, 388, 514
AfiI CCNNNNNNNGG 7 cut(s) 118, 184, 185, 340, 341, 373, 542
AflIII ACRYGT 4 cut(s) 138, 492, 494, 560
AluBI AGCT 4 cut(s) 23, 256, 292, 529
AluI AGCT 4 cut(s) 23, 256, 292, 529
Alw21I GWGCWC 1 cut(s) 554
AlwI GGATC 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 3 cut(s) 28, 280, 312
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 430
Asp718I GGTACC 1 cut(s) 354
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 3 cut(s) 114, 280, 349
AsuC2I CCSGG 3 cut(s) 284, 311, 335
AsuHPI GGTGA 5 cut(s) 67, 200, 454, 525, 557
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 2 cut(s) 114, 349
BaeI ACNNNNGTAYC 4 cut(s) 346, 379, 504, 537
BanI GGYRCC 2 cut(s) 354, 436
BanII GRGCYC 2 cut(s) 289, 347
BbrPI CACGTG 2 cut(s) 139, 495
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 1 cut(s) 85
BccI CCATC 1 cut(s) 606
BcnI CCSGG 3 cut(s) 284, 311, 335
BfaI CTAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 440
BisI GCNGC 2 cut(s) 99, 269
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 2 cut(s) 100, 270
Bme1390I CCNGG 3 cut(s) 284, 311, 335
Bme18I GGWCC 2 cut(s) 114, 349
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 3 cut(s) 114, 280, 349
BmiI GGNNCC 5 cut(s) 204, 332, 356, 438, 617
BmrFI CCNGG 3 cut(s) 284, 311, 335
BmsI GCATC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 236
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 9, 17
BpuMI CCSGG 3 cut(s) 284, 311, 335
BsaAI YACGTR 3 cut(s) 139, 495, 561
BsaHI GRCGYC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 351
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 7 cut(s) 118, 184, 185, 340, 341, 373, 542
Bse118I RCCGGY 5 cut(s) 94, 111, 366, 439, 517
Bse1I ACTGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 2 cut(s) 163, 217
BseLI CCNNNNNNNGG 7 cut(s) 118, 184, 185, 340, 341, 373, 542
BseMII CTCAG 2 cut(s) 302, 350
BseNI ACTGG 1 cut(s) 216
BseXI GCAGC 1 cut(s) 85
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 3 cut(s) 30, 282, 314
BshNI GGYRCC 2 cut(s) 354, 436
BsiHKAI GWGCWC 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 9 cut(s) 95, 112, 283, 311, 335, 352, 367, 440, 518
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 7 cut(s) 118, 184, 185, 340, 341, 373, 542
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 3 cut(s) 30, 282, 314
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 3 cut(s) 289, 347, 554
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 2 cut(s) 6, 171
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 2 cut(s) 268, 542
BspANI GGCC 3 cut(s) 30, 282, 314
BspCNI CTCAG 2 cut(s) 301, 351
BspLI GGNNCC 5 cut(s) 204, 332, 356, 438, 617
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 2 cut(s) 354, 436
BsrFI RCCGGY 5 cut(s) 94, 111, 366, 439, 517
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 5 cut(s) 94, 111, 366, 439, 517
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 6, 171
BssNI GRCGYC 1 cut(s) 437
Bst4CI ACNGT 3 cut(s) 13, 386, 505
Bst6I CTCTTC 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 3 cut(s) 139, 495, 561
BstDEI CTNAG 3 cut(s) 264, 288, 359
BstF5I GGATG 2 cut(s) 163, 217
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 2 cut(s) 9, 174
BstMBI GATC 2 cut(s) 6, 171
BstSCI CCNGG 3 cut(s) 282, 309, 333
BstV1I GCAGC 1 cut(s) 85
BsuRI GGCC 3 cut(s) 30, 282, 314
BtsCI GGATG 2 cut(s) 163, 217
BtsIMutI CAGTG 1 cut(s) 18
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 5 cut(s) 94, 111, 366, 439, 517
Cfr13I GGNCC 3 cut(s) 114, 280, 349
CpoI CGGWCCG 1 cut(s) 349
Csp6I GTAC 4 cut(s) 142, 355, 387, 513
CspI CGGWCCG 1 cut(s) 349
CviAII CATG 4 cut(s) 108, 183, 398, 620
CviQI GTAC 4 cut(s) 142, 355, 387, 513
DdeI CTNAG 3 cut(s) 264, 288, 359
DinI GGCGCC 1 cut(s) 438
DpnI GATC 2 cut(s) 8, 173
DpnII GATC 2 cut(s) 6, 171
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 30
Eco24I GRGCYC 2 cut(s) 289, 347
Eco32I GATATC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 114, 349
Eco57I CTGAAG 1 cut(s) 208
Eco72I CACGTG 2 cut(s) 139, 495
Eco88I CYCGRG 1 cut(s) 118
EcoRV GATATC 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 289, 347
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
FaeI CATG 4 cut(s) 111, 186, 401, 623
FaiI YATR 8 cut(s) 109, 184, 245, 247, 275, 318, 399, 621
FaqI GGGAC 1 cut(s) 395
FatI CATG 4 cut(s) 107, 182, 397, 619
FauI CCCGC 1 cut(s) 535
Fnu4HI GCNGC 2 cut(s) 99, 269
FokI GGATG 2 cut(s) 170, 224
FriOI GRGCYC 2 cut(s) 289, 347
Fsp4HI GCNGC 2 cut(s) 99, 269
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 438
GluI GCNGC 2 cut(s) 99, 269
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 236
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 3 cut(s) 30, 282, 314
HapII CCGG 9 cut(s) 95, 112, 283, 311, 335, 352, 367, 440, 518
HhaI GCGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 4 cut(s) 111, 186, 401, 623
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HpaII CCGG 9 cut(s) 95, 112, 283, 311, 335, 352, 367, 440, 518
HphI GGTGA 5 cut(s) 67, 200, 454, 525, 557
Hpy166II GTNNAC 3 cut(s) 142, 409, 469
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 2 cut(s) 34, 209
Hpy8I GTNNAC 3 cut(s) 142, 409, 469
Hpy99I CGWCG 2 cut(s) 128, 343
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 3 cut(s) 13, 386, 505
HpyCH4IV ACGT 4 cut(s) 138, 411, 494, 560
HpyCH4V TGCA 1 cut(s) 243
HpyF3I CTNAG 3 cut(s) 264, 288, 359
HpySE526I ACGT 4 cut(s) 138, 411, 494, 560
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 4 cut(s) 111, 186, 401, 623
HspAI GCGC 1 cut(s) 437
KasI GGCGCC 1 cut(s) 436
KpnI GGTACC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 6, 171
LmnI GCTCC 2 cut(s) 557, 615
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 378
MaeII ACGT 4 cut(s) 138, 411, 494, 560
MaeIII GTNAC 4 cut(s) 188, 362, 556, 578
MalI GATC 2 cut(s) 8, 173
MboI GATC 2 cut(s) 6, 171
MboII GAAGA 3 cut(s) 33, 70, 214
MhlI GDGCHC 3 cut(s) 289, 347, 554
MluCI AATT 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 7 cut(s) 41, 89, 127, 144, 216, 447, 595
MseI TTAA 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 292
MspI CCGG 9 cut(s) 95, 112, 283, 311, 335, 352, 367, 440, 518
MspR9I CCNGG 3 cut(s) 284, 311, 335
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 3 cut(s) 284, 311, 335
NdeII GATC 2 cut(s) 6, 171
NlaIII CATG 4 cut(s) 111, 186, 401, 623
NlaIV GGNNCC 5 cut(s) 204, 332, 356, 438, 617
NmuCI GTSAC 2 cut(s) 188, 578
PceI AGGCCT 1 cut(s) 30
PkrI GCNGC 2 cut(s) 100, 270
PluTI GGCGCC 1 cut(s) 440
PmaCI CACGTG 2 cut(s) 139, 495
PmlI CACGTG 2 cut(s) 139, 495
Ppu21I YACGTR 3 cut(s) 139, 495, 561
PspCI CACGTG 2 cut(s) 139, 495
PspFI CCCAGC 1 cut(s) 292
PspN4I GGNNCC 5 cut(s) 204, 332, 356, 438, 617
PspPI GGNCC 3 cut(s) 114, 280, 349
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 4 cut(s) 143, 356, 388, 514
RsaNI GTAC 4 cut(s) 142, 355, 387, 513
Rsr2I CGGWCCG 1 cut(s) 349
RsrII CGGWCCG 1 cut(s) 349
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 2 cut(s) 99, 269
Sau3AI GATC 2 cut(s) 6, 171
Sau96I GGNCC 3 cut(s) 114, 280, 349
ScrFI CCNGG 3 cut(s) 284, 311, 335
SduI GDGCHC 3 cut(s) 289, 347, 554
SfaNI GCATC 1 cut(s) 202
SfoI GGCGCC 1 cut(s) 438
SgrAI CRCCGGYG 2 cut(s) 439, 517
SinI GGWCC 2 cut(s) 114, 349
SmlI CTYRAG 2 cut(s) 24, 32
SmoI CTYRAG 2 cut(s) 24, 32
Sse9I AATT 1 cut(s) 430
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 2 cut(s) 268, 542
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 1 cut(s) 378
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 282, 309, 333
TaaI ACNGT 3 cut(s) 13, 386, 505
TaiI ACGT 4 cut(s) 141, 414, 497, 563
TaqI TCGA 1 cut(s) 482
TaqII GACCGA 1 cut(s) 337
TasI AATT 1 cut(s) 430
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 188, 578
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 2 cut(s) 188, 578
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 114, 349
XapI RAATTY 1 cut(s) 430
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.