MD00G1116300.v1.1

VQ motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012106
Physical Location & Seq
Reverse (-)
24581389 .. 24582256
868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1116300.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGAATCCTCACCCAGGCACCACCAACAGGATAACCCAACTCCATCTTTGCTTCCCTCCCCAAACAGCCACAGCTCCAACAGCAGCACGAGCAGCTCCAACAACAACCGTCACCTCAACGGCGGAGCTCCGCCAACAACCCCGACTCCCCATCCTCCTATACCCTACACCAGATCCGAAACCGCCAACCCGTACCCGACCACCTTTGTCCAAGCTGACACCTCCTCCTTCAAACAAGTCGTCCAAATGCTCACCGGCTCCTCCGAAACCGCTAAGCAAGCCTCTTCGAAACCCGCGTCCGACCCGCACCCGAAAACCCACATCCCACCCATCAAATCCACGCCCACAAAGCAACAATCCGGATCCGGTTTCAAGCTCTACGAGCGCCGGAACTCCCTCAAGAACCTCCGGATTAACCCATTAATCCCCGTCTCCAGCTCCGCAGCTTCCGGATTCTCGCCGCGGAATCCCGAGATTTTATCTCCGAGCATCCTCGATTTTCCTGCTCTGGTTCTCAGCCCTGTCACGCCGCTGATACCCGACCCGTTTGACCGGTCCGGGACGCCGAGTTACGGCCCGAATCCAAATGGGTGTTCGAATTTGAACAGGGAGGCGGAAGAGAAGGCTATTAAAGAAAAGGGTTTTTACTTTCACCCGTCGCCGAGCACGACGCCGCGGGAATCTGAGCCCCGACTGCTGCCTCTGTTCCCGACGACGTCGCCGAGAGCTGCTTCAGGTAAATTGTAA

Protein Analysis

249

Amino Acids

26.69

Weight (kDa)

9.64

Isoelectric Point (pI)

62.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 64 - 90 1.9e-11 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28280 AT1G28280
fragaria_vesca FvH4_3g09030 FvH4_3g09030 FvH4_3g09030 FvH4_3g09030
malus_domestica MD00G1116300.v1.1 MD10G1293000.v1.1
prunus_persica Prupe.4G048900_v2.0.a1
pyrus_communis pycom10g24500
rosa_chinensis RchiOBHm_Chr5g0008641
rosa_laevigata RLG00000031589
rosa_multiflora Rmu_sc0001665.1_g000029
rosa_rugosa Rorug04G0437600
rosa_samantha Rh5AG066200 Rh5BG063500 Rh5CG073700 Rh5DG062700
rosa_wichuraiana Rw5G006280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 18
AccII CGCG 3 cut(s) 296, 463, 676
AccIII TCCGGA 3 cut(s) 359, 408, 449
AclWI GGATC 3 cut(s) 168, 357, 370
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 3 cut(s) 563, 671, 716
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 15, 28, 572
AgeI ACCGGT 1 cut(s) 552
AgsI TTSAA 3 cut(s) 232, 373, 604
AjnI CCWGG 1 cut(s) 14
AjuI GAANNNNNNNTTGG 2 cut(s) 577, 609
AluBI AGCT 8 cut(s) 75, 96, 128, 215, 376, 438, 446, 728
AluI AGCT 8 cut(s) 75, 96, 128, 215, 376, 438, 446, 728
Alw21I GWGCWC 2 cut(s) 130, 668
Alw26I GTCTC 1 cut(s) 436
AlwI GGATC 3 cut(s) 168, 357, 370
Ama87I CYCGRG 1 cut(s) 470
Aor13HI TCCGGA 3 cut(s) 359, 408, 449
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 5 cut(s) 84, 93, 443, 697, 728
ApoI RAATTY 1 cut(s) 598
ArsI GACNNNNNNTTYG 4 cut(s) 225, 257, 281, 313
AseI ATTAAT 1 cut(s) 422
AsiGI ACCGGT 1 cut(s) 552
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 555, 575
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 4 cut(s) 3, 104, 244, 644
AsuII TTCGAA 2 cut(s) 287, 596
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 555
BamHI GGATCC 1 cut(s) 362
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 2 cut(s) 130, 690
BauI CACGAG 1 cut(s) 88
Bbv12I GWGCWC 2 cut(s) 130, 668
BbvI GCAGC 5 cut(s) 96, 105, 455, 684, 715
BccI CCATC 3 cut(s) 52, 159, 338
BceAI ACGGC 2 cut(s) 136, 589
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 1 cut(s) 16
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 1 cut(s) 436
BfoI RGCGCY 1 cut(s) 388
BisI GCNGC 8 cut(s) 85, 94, 444, 461, 530, 674, 698, 729
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 8 cut(s) 86, 95, 445, 462, 531, 675, 699, 730
Bme1390I CCNGG 2 cut(s) 16, 559
Bme18I GGWCC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 555, 575
BmiI GGNNCC 3 cut(s) 20, 259, 364
BmrFI CCNGG 2 cut(s) 16, 559
BmsI GCATC 1 cut(s) 498
BpmI CTGGAG 1 cut(s) 418
Bpu1102I GCTNAGC 1 cut(s) 273
Bpu14I TTCGAA 2 cut(s) 287, 596
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 559
BsaHI GRCGYC 3 cut(s) 563, 671, 716
BsaJI CCNNGG 3 cut(s) 14, 461, 674
BsaWI WCCGGW 5 cut(s) 359, 365, 408, 449, 552
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 209, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 28, 572
Bse118I RCCGGY 2 cut(s) 254, 552
BseAI TCCGGA 3 cut(s) 359, 408, 449
BseBI CCWGG 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 14, 461, 674
BseGI GGATG 3 cut(s) 151, 321, 489
BseLI CCNNNNNNNGG 3 cut(s) 15, 28, 572
BseMII CTCAG 2 cut(s) 529, 675
BseRI GAGGAG 2 cut(s) 214, 250
BseXI GCAGC 5 cut(s) 96, 105, 455, 684, 715
Bsh1236I CGCG 3 cut(s) 296, 463, 676
BshFI GGCC 1 cut(s) 576
BshNI GGYRCC 1 cut(s) 18
BshTI ACCGGT 1 cut(s) 552
BsiHKAI GWGCWC 2 cut(s) 130, 668
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 8 cut(s) 255, 360, 366, 388, 409, 450, 553, 558
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 3 cut(s) 15, 28, 572
BsmAI GTCTC 1 cut(s) 436
BsmBI CGTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 574
BsnI GGCC 1 cut(s) 576
BsoBI CYCGRG 1 cut(s) 470
Bsp119I TTCGAA 2 cut(s) 287, 596
Bsp1286I GDGCHC 3 cut(s) 130, 668, 690
Bsp13I TCCGGA 3 cut(s) 359, 408, 449
Bsp143I GATC 2 cut(s) 173, 362
Bsp1720I GCTNAGC 1 cut(s) 273
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 2 cut(s) 528, 676
BspEI TCCGGA 3 cut(s) 359, 408, 449
BspFNI CGCG 3 cut(s) 296, 463, 676
BspLI GGNNCC 3 cut(s) 20, 259, 364
BspPI GGATC 3 cut(s) 168, 357, 370
BspT104I TTCGAA 2 cut(s) 287, 596
BspT107I GGYRCC 1 cut(s) 18
BsrFI RCCGGY 2 cut(s) 254, 552
BssAI RCCGGY 2 cut(s) 254, 552
BssECI CCNNGG 3 cut(s) 14, 461, 674
BssMI GATC 2 cut(s) 173, 362
BssNI GRCGYC 3 cut(s) 563, 671, 716
BssSI CACGAG 1 cut(s) 88
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 110
Bst6I CTCTTC 2 cut(s) 289, 612
BstACI GRCGYC 3 cut(s) 563, 671, 716
BstBI TTCGAA 2 cut(s) 287, 596
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 3 cut(s) 273, 515, 684
BstDSI CCRYGG 2 cut(s) 461, 674
BstENI CCTNNNNNAGG 1 cut(s) 13
BstF5I GGATG 3 cut(s) 151, 321, 489
BstFNI CGCG 3 cut(s) 296, 463, 676
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 2 cut(s) 176, 365
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 2 cut(s) 173, 362
BstMWI GCNNNNNNNGC 7 cut(s) 81, 90, 93, 278, 349, 382, 694
BstNI CCWGG 1 cut(s) 16
BstSCI CCNGG 2 cut(s) 14, 557
BstUI CGCG 3 cut(s) 296, 463, 676
BstV1I GCAGC 5 cut(s) 96, 105, 455, 684, 715
BstX2I RGATCY 2 cut(s) 173, 362
BstYI RGATCY 2 cut(s) 173, 362
BsuRI GGCC 1 cut(s) 576
BtgI CCRYGG 2 cut(s) 461, 674
BtsCI GGATG 3 cut(s) 151, 321, 489
Cac8I GCNNGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 2 cut(s) 254, 552
Cfr13I GGNCC 2 cut(s) 555, 575
Cfr42I CCGCGG 2 cut(s) 464, 677
CpoI CGGWCCG 1 cut(s) 555
CseI GACGC 3 cut(s) 285, 571, 679
Csp6I GTAC 1 cut(s) 193
CspAI ACCGGT 1 cut(s) 552
CspI CGGWCCG 1 cut(s) 555
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 273, 515, 684
DpnI GATC 2 cut(s) 175, 364
DpnII GATC 2 cut(s) 173, 362
Eam1104I CTCTTC 2 cut(s) 289, 612
EarI CTCTTC 2 cut(s) 289, 612
EciI GGCGGA 3 cut(s) 120, 138, 629
Ecl136II GAGCTC 1 cut(s) 128
Eco24I GRGCYC 2 cut(s) 130, 690
Eco47I GGWCC 1 cut(s) 555
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 14
EcoT38I GRGCYC 2 cut(s) 130, 690
Esp3I CGTCTC 1 cut(s) 436
FaiI YATR 1 cut(s) 161
FaqI GGGAC 1 cut(s) 574
FauI CCCGC 3 cut(s) 301, 312, 669
Fnu4HI GCNGC 8 cut(s) 85, 94, 444, 461, 530, 674, 698, 729
FokI GGATG 3 cut(s) 138, 308, 476
FriOI GRGCYC 2 cut(s) 130, 690
Fsp4HI GCNGC 8 cut(s) 85, 94, 444, 461, 530, 674, 698, 729
GlaI GCGC 1 cut(s) 386
GluI GCNGC 8 cut(s) 85, 94, 444, 461, 530, 674, 698, 729
GsuI CTGGAG 1 cut(s) 418
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 1 cut(s) 576
HapII CCGG 8 cut(s) 255, 360, 366, 388, 409, 450, 553, 558
HgaI GACGC 3 cut(s) 285, 571, 679
HhaI GCGC 1 cut(s) 387
Hin1I GRCGYC 3 cut(s) 563, 671, 716
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HinfI GANTC 6 cut(s) 5, 145, 453, 466, 580, 680
HpaII CCGG 8 cut(s) 255, 360, 366, 388, 409, 450, 553, 558
HphI GGTGA 4 cut(s) 3, 104, 244, 644
Hpy188I TCNGA 5 cut(s) 178, 265, 301, 486, 685
Hpy188III TCNNGA 6 cut(s) 360, 400, 409, 450, 470, 709
Hpy99I CGWCG 5 cut(s) 661, 673, 715, 718, 721
HpyAV CCTTC 2 cut(s) 238, 616
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4IV ACGT 1 cut(s) 716
HpyF10VI GCNNNNNNNGC 7 cut(s) 81, 90, 93, 278, 349, 382, 694
HpyF3I CTNAG 3 cut(s) 273, 515, 684
HpySE526I ACGT 1 cut(s) 716
Hsp92I GRCGYC 3 cut(s) 563, 671, 716
HspAI GCGC 1 cut(s) 385
Kpn2I TCCGGA 3 cut(s) 359, 408, 449
KspI CCGCGG 2 cut(s) 464, 677
Kzo9I GATC 2 cut(s) 173, 362
LmnI GCTCC 6 cut(s) 80, 101, 125, 133, 263, 443
Lsp1109I GCAGC 5 cut(s) 96, 105, 455, 684, 715
LweI GCATC 1 cut(s) 498
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 3 cut(s) 110, 523, 569
MalI GATC 2 cut(s) 175, 364
MboI GATC 2 cut(s) 173, 362
MboII GAAGA 2 cut(s) 276, 629
MflI RGATCY 2 cut(s) 173, 362
MhlI GDGCHC 3 cut(s) 130, 668, 690
MluCI AATT 2 cut(s) 598, 740
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 3 cut(s) 102, 123, 324
MroI TCCGGA 3 cut(s) 359, 408, 449
MseI TTAA 3 cut(s) 414, 422, 630
MspA1I CMGCKG 3 cut(s) 463, 532, 676
MspI CCGG 8 cut(s) 255, 360, 366, 388, 409, 450, 553, 558
MspR9I CCNGG 2 cut(s) 16, 559
MvaI CCWGG 1 cut(s) 16
MvnI CGCG 3 cut(s) 296, 463, 676
MwoI GCNNNNNNNGC 7 cut(s) 81, 90, 93, 278, 349, 382, 694
NciI CCSGG 1 cut(s) 559
NdeII GATC 2 cut(s) 173, 362
NlaIV GGNNCC 3 cut(s) 20, 259, 364
NmeAIII GCCGAG 3 cut(s) 591, 687, 747
NmuCI GTSAC 2 cut(s) 110, 523
NspV TTCGAA 2 cut(s) 287, 596
PcsI WCGNNNNNNNCGW 3 cut(s) 293, 665, 719
PfeI GAWTC 5 cut(s) 5, 453, 466, 580, 680
PflFI GACNNNGTC 1 cut(s) 715
PfoI TCCNGGA 1 cut(s) 557
PinAI ACCGGT 1 cut(s) 552
PkrI GCNGC 8 cut(s) 86, 95, 445, 462, 531, 675, 699, 730
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PshBI ATTAAT 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 14
PspN4I GGNNCC 3 cut(s) 20, 259, 364
PspPI GGNCC 2 cut(s) 555, 575
PsuI RGATCY 2 cut(s) 173, 362
PsyI GACNNNGTC 1 cut(s) 715
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
Rsr2I CGGWCCG 1 cut(s) 555
RsrII CGGWCCG 1 cut(s) 555
SacI GAGCTC 1 cut(s) 130
SacII CCGCGG 2 cut(s) 464, 677
SaqAI TTAA 3 cut(s) 414, 422, 630
SatI GCNGC 8 cut(s) 85, 94, 444, 461, 530, 674, 698, 729
Sau3AI GATC 2 cut(s) 173, 362
Sau96I GGNCC 2 cut(s) 555, 575
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 2 cut(s) 16, 559
SduI GDGCHC 3 cut(s) 130, 668, 690
SfaNI GCATC 1 cut(s) 498
Sfr303I CCGCGG 2 cut(s) 464, 677
SfuI TTCGAA 2 cut(s) 287, 596
SgrBI CCGCGG 2 cut(s) 464, 677
SinI GGWCC 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 2 cut(s) 598, 740
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 2 cut(s) 14, 557
TaaI ACNGT 1 cut(s) 110
TaiI ACGT 1 cut(s) 719
TaqI TCGA 3 cut(s) 287, 495, 596
TasI AATT 2 cut(s) 598, 740
TauI GCSGC 3 cut(s) 463, 532, 676
TfiI GAWTC 5 cut(s) 5, 453, 466, 580, 680
Tru1I TTAA 3 cut(s) 414, 422, 630
Tru9I TTAA 3 cut(s) 414, 422, 630
TseFI GTSAC 2 cut(s) 110, 523
TseI GCWGC 5 cut(s) 84, 93, 443, 697, 728
Tsp45I GTSAC 2 cut(s) 110, 523
Tth111I GACNNNGTC 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 555
VspI ATTAAT 1 cut(s) 422
XagI CCTNNNNNAGG 1 cut(s) 13
XapI RAATTY 1 cut(s) 598
ZraI GACGTC 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.