Rh5AG066200

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012106
Physical Location & Seq
Forward (+)
5562311 .. 5563893
1583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG066200.1

Sequence Viewer

Length: 735 bp
ATGGAAACCTCTCCCAGAAGAGAGACTAACCCTAACCCAAACGCTCTCCCATCCCCAAACAGCAGCAGCACAAGCAGCACCAACAGCAACAACAATGGACTCCAAGCTCCCCCAACAACCCCAACCGCCCAACCCCCGAAACCCATGACCAGATCCGACTCCGCTAACCCTTACCCGACCACCTTCGTCCAGGCCGACACCACATCTTTCAAACAAGTCGTCCAAATGCTGACCGGATCCTCCGAAACAGCCAACAAGGTCGCGTGTTCGAAACACGATGCCAGACACAACATCCCGCCGATCAAGTCCACCCCCAAGAAGCAGCAACAGCAACAGAACTCCGGTTTCAAGCTCTACGAGCGCCGCAACAACGCCCTGAAGAATCTCCGGCTCAACCCTCTTATCCCAATCCCGGTGTTCAATTCCAACAACAACAACAACTCCGGGTTCTCGCCGCGAAACCCGGAGATTTTGTCGCCGAGCATTCTCGATTTCCCGGCGCTGGTGCTCAGCCCCGTCACGCCGCTCATACCCGACCCGTTTGACCGGGCCGGGTCGGCGAATTTCGGGCACGGCTGCTCACAGTTGAACAAGGAGGCCGAAGAGAAAGCGATCAAAGAAAAAGGGTTTTACTTGCATCCGTCGCCGACTACGACGCCGAGGGAATCGGAGCCCCGATTGTTGCCCTTGTTCCCGACGACGTCGCCTAGAGCTTCAGGTTCATCTTCTTCTTGA

Protein Analysis

244

Amino Acids

26.47

Weight (kDa)

9.69

Isoelectric Point (pI)

61.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 57 - 83 8.1e-11 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28280 AT1G28280
fragaria_vesca FvH4_3g09030 FvH4_3g09030 FvH4_3g09030 FvH4_3g09030
malus_domestica MD00G1116300.v1.1 MD10G1293000.v1.1
prunus_persica Prupe.4G048900_v2.0.a1
pyrus_communis pycom10g24500
rosa_chinensis RchiOBHm_Chr5g0008641
rosa_laevigata RLG00000031589
rosa_multiflora Rmu_sc0001665.1_g000029
rosa_rugosa Rorug04G0437600
rosa_samantha Rh5AG066200 Rh5BG063500 Rh5CG073700 Rh5DG062700
rosa_wichuraiana Rw5G006280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 704
AccBSI CCGCTC 1 cut(s) 526
AccII CGCG 2 cut(s) 263, 457
AciI CCGC 6 cut(s) 126, 162, 296, 364, 455, 524
AclWI GGATC 3 cut(s) 147, 231, 244
AcsI RAATTY 1 cut(s) 562
AcuI CTGAAG 2 cut(s) 398, 699
AcyI GRCGYC 2 cut(s) 656, 701
AfiI CCNNNNNNNGG 2 cut(s) 412, 502
AgsI TTSAA 4 cut(s) 211, 349, 421, 589
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 3 cut(s) 107, 352, 713
AluI AGCT 3 cut(s) 107, 352, 713
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 3 cut(s) 147, 231, 244
AoxI GGCC 3 cut(s) 192, 549, 597
ApeKI GCWGC 5 cut(s) 63, 66, 75, 322, 576
ApoI RAATTY 1 cut(s) 562
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspLEI GCGC 2 cut(s) 363, 502
AspS9I GGNCC 1 cut(s) 549
AsuC2I CCSGG 6 cut(s) 413, 445, 464, 497, 548, 553
AsuII TTCGAA 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 573
BamHI GGATCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 5 cut(s) 75, 78, 87, 334, 563
BccI CCATC 1 cut(s) 58
BceAI ACGGC 1 cut(s) 589
BciT130I CCWGG 1 cut(s) 191
BcnI CCSGG 6 cut(s) 413, 445, 464, 497, 548, 553
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 1 cut(s) 708
BfoI RGCGCY 2 cut(s) 364, 503
BglI GCCNNNNNGGC 1 cut(s) 557
BisI GCNGC 8 cut(s) 64, 67, 76, 323, 364, 455, 524, 577
BlpI GCTNAGC 1 cut(s) 509
BlsI GCNGC 8 cut(s) 65, 68, 77, 324, 365, 456, 525, 578
Bme1390I CCNGG 7 cut(s) 191, 413, 445, 464, 497, 548, 553
BmgT120I GGNCC 1 cut(s) 549
BmiI GGNNCC 2 cut(s) 238, 672
BmrFI CCNGG 7 cut(s) 191, 413, 445, 464, 497, 548, 553
BmsI GCATC 2 cut(s) 268, 646
Bpu1102I GCTNAGC 1 cut(s) 509
Bpu14I TTCGAA 1 cut(s) 269
BpuMI CCSGG 6 cut(s) 413, 445, 464, 497, 548, 553
BsaHI GRCGYC 2 cut(s) 656, 701
BsaJI CCNNGG 1 cut(s) 659
BsaWI WCCGGW 2 cut(s) 233, 341
BsaXI ACNNNNNCTCC 4 cut(s) 425, 455, 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 502
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 659
BseGI GGATG 3 cut(s) 50, 291, 637
BseLI CCNNNNNNNGG 2 cut(s) 412, 502
BseMII CTCAG 1 cut(s) 523
BseSI GKGCMC 1 cut(s) 573
BseXI GCAGC 5 cut(s) 75, 78, 87, 334, 563
Bsh1236I CGCG 2 cut(s) 263, 457
BshFI GGCC 3 cut(s) 194, 551, 599
BsiHKAI GWGCWC 1 cut(s) 510
BsiSI CCGG 9 cut(s) 234, 342, 388, 413, 444, 464, 497, 547, 552
BslI CCNNNNNNNGG 2 cut(s) 412, 502
BsmAI GTCTC 1 cut(s) 17
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 3 cut(s) 194, 551, 599
Bsp119I TTCGAA 1 cut(s) 269
Bsp1286I GDGCHC 3 cut(s) 510, 573, 675
Bsp143I GATC 4 cut(s) 152, 236, 300, 612
Bsp1720I GCTNAGC 1 cut(s) 509
BspACI CCGC 6 cut(s) 126, 162, 296, 364, 455, 524
BspANI GGCC 3 cut(s) 194, 551, 599
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 2 cut(s) 263, 457
BspLI GGNNCC 2 cut(s) 238, 672
BspPI GGATC 3 cut(s) 147, 231, 244
BspT104I TTCGAA 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 526
BssECI CCNNGG 1 cut(s) 659
BssMI GATC 4 cut(s) 152, 236, 300, 612
BssNI GRCGYC 2 cut(s) 656, 701
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 585
Bst6I CTCTTC 2 cut(s) 13, 597
BstACI GRCGYC 2 cut(s) 656, 701
BstBI TTCGAA 1 cut(s) 269
BstDEI CTNAG 1 cut(s) 509
BstF5I GGATG 3 cut(s) 50, 291, 637
BstFNI CGCG 2 cut(s) 263, 457
BstH2I RGCGCY 2 cut(s) 364, 503
BstHHI GCGC 2 cut(s) 363, 502
BstKTI GATC 4 cut(s) 155, 239, 303, 615
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 4 cut(s) 152, 236, 300, 612
BstMWI GCNNNNNNNGC 7 cut(s) 72, 75, 84, 328, 358, 557, 643
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 7 cut(s) 189, 411, 443, 462, 495, 546, 551
BstSLI GKGCMC 1 cut(s) 573
BstUI CGCG 2 cut(s) 263, 457
BstV1I GCAGC 5 cut(s) 75, 78, 87, 334, 563
BstX2I RGATCY 2 cut(s) 152, 236
BstYI RGATCY 2 cut(s) 152, 236
BsuRI GGCC 3 cut(s) 194, 551, 599
BtsCI GGATG 3 cut(s) 50, 291, 637
CfoI GCGC 2 cut(s) 363, 502
Cfr13I GGNCC 1 cut(s) 549
CseI GACGC 1 cut(s) 664
CviAII CATG 1 cut(s) 145
DdeI CTNAG 1 cut(s) 509
DpnI GATC 4 cut(s) 154, 238, 302, 614
DpnII GATC 4 cut(s) 152, 236, 300, 612
Eam1104I CTCTTC 2 cut(s) 13, 597
EarI CTCTTC 2 cut(s) 13, 597
Eco24I GRGCYC 1 cut(s) 675
Eco57I CTGAAG 2 cut(s) 398, 699
EcoRII CCWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 675
FaeI CATG 1 cut(s) 148
FaiI YATR 2 cut(s) 146, 530
FatI CATG 1 cut(s) 144
FauI CCCGC 1 cut(s) 303
Fnu4HI GCNGC 8 cut(s) 64, 67, 76, 323, 364, 455, 524, 577
FokI GGATG 3 cut(s) 37, 278, 624
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 8 cut(s) 64, 67, 76, 323, 364, 455, 524, 577
FspBI CTAG 1 cut(s) 708
GlaI GCGC 2 cut(s) 362, 501
GluI GCNGC 8 cut(s) 64, 67, 76, 323, 364, 455, 524, 577
HaeII RGCGCY 2 cut(s) 364, 503
HaeIII GGCC 3 cut(s) 194, 551, 599
HapII CCGG 9 cut(s) 234, 342, 388, 413, 444, 464, 497, 547, 552
HgaI GACGC 1 cut(s) 664
HhaI GCGC 2 cut(s) 363, 502
Hin1I GRCGYC 2 cut(s) 656, 701
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 2 cut(s) 361, 500
HinP1I GCGC 2 cut(s) 361, 500
HinfI GANTC 4 cut(s) 99, 158, 382, 665
HpaII CCGG 9 cut(s) 234, 342, 388, 413, 444, 464, 497, 547, 552
Hpy166II GTNNAC 1 cut(s) 309
Hpy188I TCNGA 3 cut(s) 157, 244, 670
Hpy188III TCNNGA 3 cut(s) 488, 694, 732
Hpy8I GTNNAC 1 cut(s) 309
Hpy99I CGWCG 5 cut(s) 646, 658, 700, 703, 706
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 585
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 1 cut(s) 637
HpyF10VI GCNNNNNNNGC 7 cut(s) 72, 75, 84, 328, 358, 557, 643
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 1 cut(s) 701
Hsp92I GRCGYC 2 cut(s) 656, 701
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 2 cut(s) 361, 500
Kzo9I GATC 4 cut(s) 152, 236, 300, 612
LmnI GCTCC 2 cut(s) 112, 670
Lsp1109I GCAGC 5 cut(s) 75, 78, 87, 334, 563
LweI GCATC 2 cut(s) 268, 646
MaeI CTAG 1 cut(s) 708
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 1 cut(s) 517
MalI GATC 4 cut(s) 154, 238, 302, 614
MbiI CCGCTC 1 cut(s) 526
MboI GATC 4 cut(s) 152, 236, 300, 612
MboII GAAGA 5 cut(s) 30, 391, 614, 717, 720
MflI RGATCY 2 cut(s) 152, 236
MhlI GDGCHC 3 cut(s) 510, 573, 675
MluCI AATT 2 cut(s) 421, 562
MlyI GAGTC 2 cut(s) 93, 152
MmeI TCCRAC 2 cut(s) 180, 450
MnlI CCTC 5 cut(s) 19, 250, 408, 589, 654
MspI CCGG 9 cut(s) 234, 342, 388, 413, 444, 464, 497, 547, 552
MspR9I CCNGG 7 cut(s) 191, 413, 445, 464, 497, 548, 553
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 2 cut(s) 263, 457
MwoI GCNNNNNNNGC 7 cut(s) 72, 75, 84, 328, 358, 557, 643
NciI CCSGG 6 cut(s) 413, 445, 464, 497, 548, 553
NdeII GATC 4 cut(s) 152, 236, 300, 612
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 238, 672
NmeAIII GCCGAG 2 cut(s) 504, 684
NmuCI GTSAC 1 cut(s) 517
NspV TTCGAA 1 cut(s) 269
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 650
PctI GAATGC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 382, 665
PflFI GACNNNGTC 1 cut(s) 700
PkrI GCNGC 8 cut(s) 65, 68, 77, 324, 365, 456, 525, 578
PleI GAGTC 2 cut(s) 93, 152
PpsI GAGTC 2 cut(s) 93, 152
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 238, 672
PspPI GGNCC 1 cut(s) 549
PsuI RGATCY 2 cut(s) 152, 236
PsyI GACNNNGTC 1 cut(s) 700
SatI GCNGC 8 cut(s) 64, 67, 76, 323, 364, 455, 524, 577
Sau3AI GATC 4 cut(s) 152, 236, 300, 612
Sau96I GGNCC 1 cut(s) 549
SchI GAGTC 2 cut(s) 93, 152
ScrFI CCNGG 7 cut(s) 191, 413, 445, 464, 497, 548, 553
SduI GDGCHC 3 cut(s) 510, 573, 675
SetI ASST 8 cut(s) 11, 109, 185, 261, 354, 704, 715, 721
SfaNI GCATC 2 cut(s) 268, 646
SfuI TTCGAA 1 cut(s) 269
Sse9I AATT 2 cut(s) 421, 562
SsiI CCGC 6 cut(s) 126, 162, 296, 364, 455, 524
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 7 cut(s) 189, 411, 443, 462, 495, 546, 551
TaaI ACNGT 1 cut(s) 585
TaiI ACGT 1 cut(s) 704
TaqI TCGA 2 cut(s) 269, 489
TasI AATT 2 cut(s) 421, 562
TauI GCSGC 3 cut(s) 366, 457, 526
TfiI GAWTC 2 cut(s) 382, 665
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 5 cut(s) 63, 66, 75, 322, 576
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 1 cut(s) 711
TspGWI ACGGA 1 cut(s) 630
Tth111I GACNNNGTC 1 cut(s) 700
XapI RAATTY 1 cut(s) 562
XspI CTAG 1 cut(s) 708
ZraI GACGTC 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.