MD00G1126900.v1.1
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010351
Physical Location & Seq
Reverse (-)
26928950 .. 26938063
9114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1126900.v1.1.491

Sequence Viewer

Length: 2394 bp
ATGCTGAAATTCTTGAAGGGAGTGGTGGGCGGATCGGGGACTGGACCTAAGGATCTGCCCTACAACATCGGCGATCCTTACCCCTCTGCTTGGGGCTCTTGGACTCACTTCCGAGGCACCTCCAAGGACGATGGGTCTCCGGTGTCCATATTCTCTATATCTGGAAGTAATGCTCAGGATGGCCATTTGGCCGCTGCTCGCAATGGCGTCAAGCGTCTCCGCACTGTCAGACACCCAAACATCTTATCATTTCTTCACAGTACTGAGGCTGAAACTGTGGATGCTTCTTCTGCCACCAAGCAAACTATCTATATCGTTACCGAGCCTGTTATGCCCCTCTCTGATAAGATCAAGGAGCTCAATTTACAAGGTATCCAAAGGGATGAGTATTTCGCATGCGGACTGCACCAGATAGCGAAGGCTGTCAGTTTCTTGAATAATGATTGTAAACTTGTTCATGCCAACGTTTGCTTGGCCAGTGTTGTTGTAACTCAAACTTTGGACTGGAAGCTACATGCTTTTGATGTACTATCTGAGTTTGATGGCGGCAATGAGGCTTCTGCCGGAGAAATGCTGCAATTCGCCTGGCTTGTTGGACCACAATACAAACCCATGGAGTTACTGAAGTCTGATTGGGCTGCAATCAGAAAGTCTCCACCATGGGCTATTGATTCTTGGGGCTTGGGCTGCCTTATATACGAGCTCTTCTCCGGTCTGAAATTAAGCAAAACAGAGGAACTACGCAACACGGCTTCTATTCCAAAGTCTCTGCTTCCAGATTATCAGCGGCTCTTGAGTTCCACGCCTTCTCGTAGGTTGAATACATCGAAGCTTGTAGAAAATAGTGAATATTTTCAAAACAAGCTAGTGGATACAATACATTTCATGGAAATTCTAAATCTGAAAGATAGTGTTGAGAAGGATACCTTCTTCCGCAAGCTTCCAAATTTAGCGGAGCAGCTACCTCGCCAGATTGTGCTGAAAAAGTTGCTTCCTTTATTGGCTTCTGCCCTTGAATTTGGTTCAGCTGCTGCACCTGCTTTGACGGCTTTGTTGAAAATGGGTTCCTGGCTTTCAACCGAAGAATTCAGTGTGAAGGTGCTGCCAACAATTGTGAAGCTGTTTGCTTCCAATGACCGAGCTATTCGAGTTGGACTTTTGCAACATATTGATCAGTTTGGAGAATCATTATCAGCACAAATTGTTGATGAGCAAGTTTACCCCCACGTTGCTACTGGGTTCACTGACACATCTGCTTTTCTCCGGGAACTGACTTTGAAGTCGATGCTTGTTCTAGCTCCTAAGCTTTCTCAACGCACCATTTCAGGGTCCTTGTTGAAGTATCTTTCAAAGTTACAGGTTGATGAAGAACCAGCAATTAGAACGAACACCACTATATTACTAGGGAACATTGCATCCCACCTGAATGATGGGACAAGGAAAAGAGTGCTGATTAATGCTTTTACTGCCCGTGCATTACGTGATACTTTCTCACCTGCCCGAGGAGCAGGTATAATGGCTTTGTGTGCTACCAGTTCTTATTATGATATCACTGAGATTGCAACTCGGATTCTCCCAAATGTTGTTATTCTTACCATTGATCCTGATAATGATGTTCGATCAAAGGCATTTCAAGCAGTTGATCAATTTTTACAAATAGTGAAGCAATCCTATGAAAAGACTGATTCAGGAGATACTGCTGGTGTTGGAATCTCATCACTACCAGGAAACGCTAGTTTACTGGGATGGGCAATGAGCTCCTTGACTCTCAAGGGTAAACCTTCTGAACAAGCTCCACTTGCTCCTGTAAATACCAGTGCATCTCTTACTAAAACAACTTCTAATGCAAGCTCAGTGGTGGATACTCCAAGCACGGCGCCTGCCCACATAAGTTCGACAACAGATTTTGGCGATCAACGTATACCTGATTCCCCAACATCTACAGATGGCTGGGGAGAACTTGAAAATGGAACCCACGAAGAGAATGAAAGTGACAAGGATGGGTGGGATGATGTTGAACCCCTGGAAGAGCCAAAACCATCTCCTGTTCTTGCGAGTATTCAAGCAGCTCAAAAGCGGCCGGTCTCACAACCTGCTTTGCAGCCTAAACAAGCGACGAGTTTGAGACCAAAAAACACAGCCAAGGCTATAAAAAATGAAGATGATGATTTGTGGGGCTCCATTGCCGCCCCTGCACCAAGAACAGTTTCAAAACCATTGAATGTGAAAGCAAGTGCAGCAGTTGATGATGATGATCCATGGGCTGCGATTGCCGCTCCCCCACCCTCTACCAAGGCAAAGCCTCTATCAGTAGCCAAAGGCCGAGGAGCGAAACCTGCTGCTCCAAAATTGGGCGCCCAGAGGATAAACCGGACGTCTTCGTCGGGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

798

Amino Acids

86.18

Weight (kDa)

6.91

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 37 - 247 2e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1045, 1504
AasI GACNNNNNNGTC 2 cut(s) 1279, 2380
AatII GACGTC 1 cut(s) 2378
Acc36I ACCTGC 5 cut(s) 1045, 1499, 1504, 2101, 2344
AccB1I GGYRCC 3 cut(s) 116, 1876, 2354
AccBSI CCGCTC 1 cut(s) 2276
AccI GTMKAC 1 cut(s) 1921
AclI AACGTT 1 cut(s) 465
AclWI GGATC 5 cut(s) 40, 60, 68, 1595, 2249
AcoI YGGCCR 4 cut(s) 181, 189, 474, 2078
AcsI RAATTY 5 cut(s) 8, 891, 946, 1016, 1085
AcuI CTGAAG 1 cut(s) 644
AcyI GRCGYC 4 cut(s) 207, 1877, 2355, 2375
AfaI GTAC 2 cut(s) 262, 528
AfiI CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
AhdI GACNNNNNGTC 1 cut(s) 133
AjnI CCWGG 4 cut(s) 584, 1067, 1723, 2022
AjuI GAANNNNNNNTTGG 2 cut(s) 2191, 2223
AloI GAACNNNNNNTCC 2 cut(s) 1400, 1432
Alw21I GWGCWC 3 cut(s) 360, 705, 1760
Alw26I GTCTC 6 cut(s) 141, 221, 657, 771, 2089, 2119
AlwI GGATC 5 cut(s) 40, 60, 68, 1595, 2249
AlwNI CAGNNNCTG 1 cut(s) 1031
Ama87I CYCGRG 1 cut(s) 1500
AoxI GGCC 5 cut(s) 181, 189, 474, 2078, 2320
ApoI RAATTY 5 cut(s) 8, 891, 946, 1016, 1085
AseI ATTAAT 1 cut(s) 1455
Asp700I GAANNNNTTC 2 cut(s) 852, 2206
AspLEI GCGC 2 cut(s) 1879, 2357
AspS9I GGNCC 3 cut(s) 44, 596, 1329
AsuC2I CCSGG 1 cut(s) 1265
AsuHPI GGTGA 1 cut(s) 1485
AvaI CYCGRG 1 cut(s) 1500
AvaII GGWCC 3 cut(s) 44, 596, 1329
AxyI CCTNAGG 1 cut(s) 48
BalI TGGCCA 2 cut(s) 183, 476
BanI GGYRCC 3 cut(s) 116, 1876, 2354
BanII GRGCYC 5 cut(s) 98, 360, 705, 1760, 2180
BbsI GAAGAC 1 cut(s) 2370
Bbv12I GWGCWC 3 cut(s) 360, 705, 1760
BccI CCATC 8 cut(s) 125, 173, 536, 1424, 1740, 1940, 1994, 2047
BceAI ACGGC 3 cut(s) 765, 1062, 1890
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 4 cut(s) 586, 1069, 1725, 2024
BciVI GTATCC 4 cut(s) 383, 865, 916, 1855
BclI TGATCA 2 cut(s) 1171, 1642
BcnI CCSGG 1 cut(s) 1265
BcoDI GTCTC 6 cut(s) 141, 221, 657, 771, 2089, 2119
BfaI CTAG 4 cut(s) 866, 1295, 1403, 1734
BfmI CTRYAG 1 cut(s) 1941
BfoI RGCGCY 2 cut(s) 1880, 2358
BfuAI ACCTGC 5 cut(s) 1045, 1499, 1504, 2101, 2344
BfuI GTATCC 4 cut(s) 383, 865, 916, 1855
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 5 cut(s) 586, 1069, 1265, 1725, 2024
Bme18I GGWCC 3 cut(s) 44, 596, 1329
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 1500
BmgT120I GGNCC 3 cut(s) 44, 596, 1329
BmiI GGNNCC 7 cut(s) 118, 1066, 1330, 1878, 1972, 2179, 2356
BmrFI CCNGG 5 cut(s) 586, 1069, 1265, 1725, 2024
BmrI ACTGGG 2 cut(s) 1245, 1751
BmsI GCATC 4 cut(s) 271, 1275, 1424, 1829
BmuI ACTGGG 2 cut(s) 1245, 1751
BpiI GAAGAC 1 cut(s) 2370
BplI GAGNNNNNCTC 2 cut(s) 692, 724
Bpu10I CCTNAGC 2 cut(s) 174, 1302
BpuEI CTTGAG 2 cut(s) 814, 1754
BpuMI CCSGG 1 cut(s) 1265
BsaAI YACGTR 1 cut(s) 1481
BsaBI GATNNNNATC 1 cut(s) 2253
BsaHI GRCGYC 4 cut(s) 207, 1877, 2355, 2375
BsaI GGTCTC 3 cut(s) 141, 2089, 2119
BsaWI WCCGGW 3 cut(s) 139, 710, 2370
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
Bse118I RCCGGY 1 cut(s) 2080
Bse1I ACTGG 7 cut(s) 46, 477, 509, 1240, 1533, 1746, 1815
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
Bse8I GATNNNNATC 1 cut(s) 2253
BseBI CCWGG 4 cut(s) 586, 1069, 1725, 2024
BseGI GGATG 7 cut(s) 184, 286, 388, 1415, 1751, 2005, 2014
BseJI GATNNNNATC 1 cut(s) 2253
BseLI CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
BseMI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
BseMII CTCAG 5 cut(s) 188, 255, 525, 1545, 1866
BseNI ACTGG 7 cut(s) 46, 477, 509, 1240, 1533, 1746, 1815
BseRI GAGGAG 2 cut(s) 1518, 2340
BseX3I CGGCCG 1 cut(s) 2078
BseYI CCCAGC 1 cut(s) 1950
BsgI GTGCAG 4 cut(s) 389, 1017, 2178, 2256
Bsh1285I CGRYCG 1 cut(s) 2081
BshFI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
BshNI GGYRCC 3 cut(s) 116, 1876, 2354
BsiEI CGRYCG 1 cut(s) 2081
BsiHKAI GWGCWC 3 cut(s) 360, 705, 1760
BsiHKCI CYCGRG 1 cut(s) 1500
BsiSI CCGG 6 cut(s) 140, 564, 711, 1264, 2081, 2371
BslFI GGGAC 2 cut(s) 52, 1447
BslI CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
BsmAI GTCTC 6 cut(s) 141, 221, 657, 771, 2089, 2119
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 52, 1447
BsnI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
Bso31I GGTCTC 3 cut(s) 141, 2089, 2119
BsoBI CYCGRG 1 cut(s) 1500
Bsp1286I GDGCHC 5 cut(s) 98, 360, 705, 1760, 2180
Bsp19I CCATGG 3 cut(s) 612, 659, 2258
BspANI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
BspCNI CTCAG 5 cut(s) 187, 256, 526, 1546, 1865
BspLI GGNNCC 7 cut(s) 118, 1066, 1330, 1878, 1972, 2179, 2356
BspMI ACCTGC 5 cut(s) 1045, 1499, 1504, 2101, 2344
BspPI GGATC 5 cut(s) 40, 60, 68, 1595, 2249
BspQI GCTCTTC 2 cut(s) 710, 2022
BspT107I GGYRCC 3 cut(s) 116, 1876, 2354
BspTNI GGTCTC 3 cut(s) 141, 2089, 2119
BsrBI CCGCTC 1 cut(s) 2276
BsrDI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
BsrFI RCCGGY 1 cut(s) 2080
BsrI ACTGG 7 cut(s) 46, 477, 509, 1240, 1533, 1746, 1815
BssAI RCCGGY 1 cut(s) 2080
BssNAI GTATAC 1 cut(s) 1922
BssNI GRCGYC 4 cut(s) 207, 1877, 2355, 2375
BssT1I CCWWGG 6 cut(s) 123, 612, 659, 2142, 2258, 2292
Bst1107I GTATAC 1 cut(s) 1922
Bst2UI CCWGG 4 cut(s) 586, 1069, 1725, 2024
Bst4CI ACNGT 4 cut(s) 226, 260, 277, 2206
Bst6I CTCTTC 3 cut(s) 710, 1974, 2022
BstACI GRCGYC 4 cut(s) 207, 1877, 2355, 2375
BstBAI YACGTR 1 cut(s) 1481
BstC8I GCNNGC 5 cut(s) 199, 397, 938, 1849, 1881
BstDEI CTNAG 7 cut(s) 48, 174, 264, 534, 1302, 1554, 1852
BstDSI CCRYGG 3 cut(s) 612, 659, 2258
BstENI CCTNNNNNAGG 1 cut(s) 1500
BstF5I GGATG 7 cut(s) 184, 286, 388, 1415, 1751, 2005, 2014
BstH2I RGCGCY 2 cut(s) 1880, 2358
BstHHI GCGC 2 cut(s) 1879, 2357
BstMAI GTCTC 6 cut(s) 141, 221, 657, 771, 2089, 2119
BstMCI CGRYCG 1 cut(s) 2081
BstNI CCWGG 4 cut(s) 586, 1069, 1725, 2024
BstNSI RCATGY 2 cut(s) 399, 518
BstSCI CCNGG 5 cut(s) 584, 1067, 1263, 1723, 2022
BstSFI CTRYAG 1 cut(s) 1941
BstV2I GAAGAC 1 cut(s) 2370
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BstZ17I GTATAC 1 cut(s) 1922
BstZI CGGCCG 1 cut(s) 2078
Bsu36I CCTNAGG 1 cut(s) 48
BsuI GTATCC 4 cut(s) 383, 865, 916, 1855
BsuRI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
BtgI CCRYGG 3 cut(s) 612, 659, 2258
BtsCI GGATG 7 cut(s) 184, 286, 388, 1415, 1751, 2005, 2014
BtsIMutI CAGTG 7 cut(s) 222, 484, 1096, 1242, 1551, 1822, 1860
BveI ACCTGC 5 cut(s) 1045, 1499, 1504, 2101, 2344
Cac8I GCNNGC 5 cut(s) 199, 397, 938, 1849, 1881
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 2 cut(s) 1879, 2357
Cfr10I RCCGGY 1 cut(s) 2080
Cfr13I GGNCC 3 cut(s) 44, 596, 1329
CseI GACGC 2 cut(s) 196, 203
Csp6I GTAC 2 cut(s) 261, 527
CspCI CAANNNNNGTGG 2 cut(s) 1836, 1871
CviAII CATG 7 cut(s) 396, 458, 515, 613, 660, 886, 2259
CviQI GTAC 2 cut(s) 261, 527
DdeI CTNAG 7 cut(s) 48, 174, 264, 534, 1302, 1554, 1852
DinI GGCGCC 2 cut(s) 1878, 2356
DrdI GACNNNNNNGTC 2 cut(s) 1279, 2380
DriI GACNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 2 cut(s) 1279, 2380
EaeI YGGCCR 4 cut(s) 181, 189, 474, 2078
EagI CGGCCG 1 cut(s) 2078
Eam1104I CTCTTC 3 cut(s) 710, 1974, 2022
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 3 cut(s) 710, 1974, 2022
EciI GGCGGA 1 cut(s) 45
Ecl136II GAGCTC 3 cut(s) 358, 703, 1758
EclXI CGGCCG 1 cut(s) 2078
Eco130I CCWWGG 6 cut(s) 123, 612, 659, 2142, 2258, 2292
Eco24I GRGCYC 5 cut(s) 98, 360, 705, 1760, 2180
Eco31I GGTCTC 3 cut(s) 141, 2089, 2119
Eco32I GATATC 1 cut(s) 1549
Eco47I GGWCC 3 cut(s) 44, 596, 1329
Eco52I CGGCCG 1 cut(s) 2078
Eco53kI GAGCTC 3 cut(s) 358, 703, 1758
Eco57I CTGAAG 1 cut(s) 644
Eco81I CCTNAGG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 1500
EcoICRI GAGCTC 3 cut(s) 358, 703, 1758
EcoNI CCTNNNNNAGG 1 cut(s) 1500
EcoO109I RGGNCCY 1 cut(s) 1329
EcoRI GAATTC 1 cut(s) 1085
EcoRII CCWGG 4 cut(s) 584, 1067, 1723, 2022
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 6 cut(s) 123, 612, 659, 2142, 2258, 2292
EcoT38I GRGCYC 5 cut(s) 98, 360, 705, 1760, 2180
EgeI GGCGCC 2 cut(s) 1878, 2356
EheI GGCGCC 2 cut(s) 1878, 2356
ErhI CCWWGG 6 cut(s) 123, 612, 659, 2142, 2258, 2292
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 7 cut(s) 399, 461, 518, 616, 663, 889, 2262
FalI AAGNNNNNCTT 4 cut(s) 911, 943, 1782, 1814
FaqI GGGAC 2 cut(s) 52, 1447
FatI CATG 7 cut(s) 395, 457, 514, 612, 659, 885, 2258
FbaI TGATCA 2 cut(s) 1171, 1642
FblI GTMKAC 1 cut(s) 1921
FokI GGATG 7 cut(s) 191, 293, 395, 1402, 1758, 2012, 2021
FriOI GRGCYC 5 cut(s) 98, 360, 705, 1760, 2180
FspBI CTAG 4 cut(s) 866, 1295, 1403, 1734
GlaI GCGC 2 cut(s) 1878, 2356
GsaI CCCAGC 1 cut(s) 1954
HaeII RGCGCY 2 cut(s) 1880, 2358
HaeIII GGCC 5 cut(s) 183, 191, 476, 2080, 2322
HapII CCGG 6 cut(s) 140, 564, 711, 1264, 2081, 2371
HgaI GACGC 2 cut(s) 196, 203
HhaI GCGC 2 cut(s) 1879, 2357
Hin1I GRCGYC 4 cut(s) 207, 1877, 2355, 2375
Hin1II CATG 7 cut(s) 399, 461, 518, 616, 663, 889, 2262
Hin6I GCGC 2 cut(s) 1877, 2355
HinP1I GCGC 2 cut(s) 1877, 2355
HindIII AAGCTT 3 cut(s) 830, 938, 1304
HinfI GANTC 8 cut(s) 103, 671, 1184, 1570, 1685, 1710, 1765, 1928
HpaII CCGG 6 cut(s) 140, 564, 711, 1264, 2081, 2371
HphI GGTGA 1 cut(s) 1485
Hpy166II GTNNAC 6 cut(s) 449, 1219, 1242, 1739, 1778, 1922
Hpy188III TCNNGA 8 cut(s) 13, 162, 176, 433, 776, 793, 1604, 1689
Hpy8I GTNNAC 6 cut(s) 449, 1219, 1242, 1739, 1778, 1922
Hpy99I CGWCG 2 cut(s) 2119, 2386
HpyAV CCTTC 7 cut(s) 10, 412, 816, 913, 937, 1090, 1791
HpyCH4III ACNGT 4 cut(s) 226, 260, 277, 2206
HpyCH4IV ACGT 5 cut(s) 465, 1227, 1480, 1918, 2375
HpyF3I CTNAG 7 cut(s) 48, 174, 264, 534, 1302, 1554, 1852
HpySE526I ACGT 5 cut(s) 465, 1227, 1480, 1918, 2375
Hsp92I GRCGYC 4 cut(s) 207, 1877, 2355, 2375
Hsp92II CATG 7 cut(s) 399, 461, 518, 616, 663, 889, 2262
HspAI GCGC 2 cut(s) 1877, 2355
KasI GGCGCC 2 cut(s) 1876, 2354
Ksp22I TGATCA 2 cut(s) 1171, 1642
LguI GCTCTTC 2 cut(s) 710, 2022
LweI GCATC 4 cut(s) 271, 1275, 1424, 1829
MaeI CTAG 4 cut(s) 866, 1295, 1403, 1734
MaeII ACGT 5 cut(s) 465, 1227, 1480, 1918, 2375
MaeIII GTNAC 5 cut(s) 316, 487, 618, 1353, 1991
MbiI CCGCTC 1 cut(s) 2276
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 5 cut(s) 98, 360, 705, 1760, 2180
MlsI TGGCCA 2 cut(s) 183, 476
MluNI TGGCCA 2 cut(s) 183, 476
Mly113I GGCGCC 2 cut(s) 1877, 2355
MlyI GAGTC 2 cut(s) 97, 1759
MmeI TCCRAC 3 cut(s) 574, 1132, 1687
Mox20I TGGCCA 2 cut(s) 183, 476
MroXI GAANNNNTTC 2 cut(s) 852, 2206
MscI TGGCCA 2 cut(s) 183, 476
MseI TTAA 2 cut(s) 722, 1455
MslI CAYNNNNRTG 1 cut(s) 1425
Msp20I TGGCCA 2 cut(s) 183, 476
MspA1I CMGCKG 3 cut(s) 194, 787, 1028
MspI CCGG 6 cut(s) 140, 564, 711, 1264, 2081, 2371
MspR9I CCNGG 5 cut(s) 586, 1069, 1265, 1725, 2024
MunI CAATTG 1 cut(s) 1110
MvaI CCWGG 4 cut(s) 586, 1069, 1725, 2024
NarI GGCGCC 2 cut(s) 1877, 2355
NciI CCSGG 1 cut(s) 1265
NcoI CCATGG 3 cut(s) 612, 659, 2258
NlaIII CATG 7 cut(s) 399, 461, 518, 616, 663, 889, 2262
NlaIV GGNNCC 7 cut(s) 118, 1066, 1330, 1878, 1972, 2179, 2356
NmeAIII GCCGAG 1 cut(s) 2348
NmuCI GTSAC 1 cut(s) 1991
NspI RCATGY 2 cut(s) 399, 518
PaeI GCATGC 1 cut(s) 399
PaqCI CACCTGC 2 cut(s) 1045, 1504
PciSI GCTCTTC 2 cut(s) 710, 2022
PdmI GAANNNNTTC 2 cut(s) 852, 2206
PfeI GAWTC 6 cut(s) 671, 1184, 1570, 1685, 1710, 1928
PfoI TCCNGGA 1 cut(s) 1263
PleI GAGTC 2 cut(s) 97, 1759
PluTI GGCGCC 2 cut(s) 1880, 2358
PpsI GAGTC 2 cut(s) 97, 1759
Ppu21I YACGTR 1 cut(s) 1481
PpuMI RGGWCCY 1 cut(s) 1329
PshBI ATTAAT 1 cut(s) 1455
Psp124BI GAGCTC 3 cut(s) 360, 705, 1760
Psp1406I AACGTT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 1329
Psp6I CCWGG 4 cut(s) 584, 1067, 1723, 2022
PspFI CCCAGC 1 cut(s) 1950
PspGI CCWGG 4 cut(s) 584, 1067, 1723, 2022
PspN4I GGNNCC 7 cut(s) 118, 1066, 1330, 1878, 1972, 2179, 2356
PspPI GGNCC 3 cut(s) 44, 596, 1329
PspPPI RGGWCCY 1 cut(s) 1329
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 1028
RsaI GTAC 2 cut(s) 262, 528
RsaNI GTAC 2 cut(s) 261, 527
RseI CAYNNNNRTG 1 cut(s) 1425
SacI GAGCTC 3 cut(s) 360, 705, 1760
SapI GCTCTTC 2 cut(s) 710, 2022
SaqAI TTAA 2 cut(s) 722, 1455
Sau96I GGNCC 3 cut(s) 44, 596, 1329
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 2 cut(s) 97, 1759
ScrFI CCNGG 5 cut(s) 586, 1069, 1265, 1725, 2024
SduI GDGCHC 5 cut(s) 98, 360, 705, 1760, 2180
SfaNI GCATC 4 cut(s) 271, 1275, 1424, 1829
SfcI CTRYAG 1 cut(s) 1941
SfoI GGCGCC 2 cut(s) 1878, 2356
SinI GGWCC 3 cut(s) 44, 596, 1329
SmiMI CAYNNNNRTG 1 cut(s) 1425
SmlI CTYRAG 2 cut(s) 793, 1769
SmoI CTYRAG 2 cut(s) 793, 1769
SphI GCATGC 1 cut(s) 399
SspDI GGCGCC 2 cut(s) 1876, 2354
SspI AATATT 1 cut(s) 851
SspMI CTAG 4 cut(s) 866, 1295, 1403, 1734
SstI GAGCTC 3 cut(s) 360, 705, 1760
StyD4I CCNGG 5 cut(s) 584, 1067, 1263, 1723, 2022
StyI CCWWGG 6 cut(s) 123, 612, 659, 2142, 2258, 2292
TaaI ACNGT 4 cut(s) 226, 260, 277, 2206
TaiI ACGT 5 cut(s) 468, 1230, 1483, 1921, 2378
TaqI TCGA 5 cut(s) 827, 1147, 1283, 1618, 1895
TaqII GACCGA 1 cut(s) 1152
TatI WGTACW 2 cut(s) 260, 526
TauI GCSGC 6 cut(s) 194, 549, 790, 2080, 2189, 2276
TfiI GAWTC 6 cut(s) 671, 1184, 1570, 1685, 1710, 1928
Tru1I TTAA 2 cut(s) 722, 1455
Tru9I TTAA 2 cut(s) 722, 1455
TscAI CASTG 7 cut(s) 229, 484, 1096, 1249, 1558, 1822, 1860
TseFI GTSAC 1 cut(s) 1991
Tsp45I GTSAC 1 cut(s) 1991
TspDTI ATGAA 6 cut(s) 446, 874, 1380, 1689, 2001, 2172
TspRI CASTG 7 cut(s) 229, 484, 1096, 1249, 1558, 1822, 1860
VpaK11BI GGWCC 3 cut(s) 44, 596, 1329
VspI ATTAAT 1 cut(s) 1455
XagI CCTNNNNNAGG 1 cut(s) 1500
XapI RAATTY 5 cut(s) 8, 891, 946, 1016, 1085
XceI RCATGY 2 cut(s) 399, 518
XcmI CCANNNNNNNNNTGG 3 cut(s) 469, 1232, 1427
XmiI GTMKAC 1 cut(s) 1921
XmnI GAANNNNTTC 2 cut(s) 852, 2206
XspI CTAG 4 cut(s) 866, 1295, 1403, 1734
ZraI GACGTC 1 cut(s) 2376
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.