Rh4DG446100
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010351
Physical Location & Seq
Reverse (-)
64564055 .. 64575162
11108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG446100.1

Sequence Viewer

Length: 2121 bp
ATGTTGAAATTCTTGAATCGAGTGGTGGGTGGATCTGGGAGCGGACCCAAAGATCTGCCATACAACATCGGCGAGCCCTATCCCTCCGCTTGGGGTTCTTGGACACACTTCAGGGGCACCTCCAAGGACGATGGGTCTCCGGTGTCCATATTTTCCATTAGTGGAAGTAATGCTCAGGATGGCCATTTGGCCGCCGCTCGCAATGGCGTCAAGCGTCTCCGTACTGTCAGACATCCCAACATCTTATCATTTCTCCACAGTACTGAAACCGAAACCATGGATGCTTCTACCACCAAGCACACTATCTACATTGTCACCGAGCCTGTTATGCCACTCTCTGAGAAGATCAAAGAACTCTGTTTACAAAACATCCAAAGGGATGAATATTACGCTTGGGGCCTGCACCAGATTGCTAAAGCTGTTAGTTTCTTAAATAATGATTGTAAACTGGTTCATGCCAATGTTTGTGTTGCCAGTGTGGTTGTAACTCAAACCTTGGACTGGAAGCTGCATGCTTTTGATGTTCTTTCCGAGTTTGATGGCACCAATGAGGCTTCTGCTGGACCAATGCTGCAATATGCCTGGCTTGTTGGATCACAATACAAACCTGTGGAGTTGTTGAAGTCTGATTGGGCGGCAGTCAGAAAGTCTCCACCATGGGCCATTGATTCTTGGGGCTTGGGCTGTCTTATCTACGAGCTCTTCTCTGGTCTGAAGTTAAGCAAAACAGAGGAGCTGCGAAACACTGCTTCTATTCCAAAGTCTCTGCTTCCAGATTATCAGCGGCTCCTGAGTGCCGTGCCTTCTCGTAGGTTGAATACGTCGAAGCTTATAGAAAACAGTGCCTATTTTCAAAATAAGTTGGTGGATACAATACACTTCATGGAAATTCTAAATTTGAAGGATAGTGTTGAGAAAGATACTTTCTTCCGCAAGCTTCCGAATTTGGCAGAGCAGCTACCTCGTCAAATTGTGCTGAAAAAGTTACTTCCTTTATTAGCTTCTGCCCTTGAATTTGGTTCAGCTGCTGCACCTGCTTTGACTGCATTGTTAAAAATGGGTTCTTGGCTTTCAACTGAAGAATTTAGTGTGAAGGTGCTGCCAACCATTGTGAAACTGTTTGCTTCCAATGACCGAGCTATTCGAGTTGGTCTTTTGCAATATGTTGATCAGTTTGGAGAATCATTATCAGCACAAATTGTTGATGAGCAAGTTTATCCCCACGTTGCTACCGGTTTCTCTGATACATCTGCTTTTCTTCGGGAACTGACTCTAAAGTCAATGCTCGTTCTCGCTCCCAAGCTTTCTCAACGTACCATTTCAGGGTCGTTGTTGAAGTATCTTTCAAAGTTACAGGTTGATGAAGAACCTGCAATTAGAACAAATACTACTATATTATTAGGGAACATTGCAAGCCACCTGAATGAAGGGACAAGGAAAAGAGTTTTGATTAATGCATTTACTGTTCGTGCATTACGGGATAGTTTCTCACCTGCCCGAGGAGCAGGGATTATGGCGTTATGTGCAACCAGCTCTTATTATGACATCACTGAGATTTCAACTAGGATCCTCCCAAATGTTGTTGTTCTAATCATTGACCCTGACAATGATGTTCGATCGAAGGCATTTCAAGCAGTTGATCAGTTTCTACAGATAGTGAAGCAAACCTACGAGAAGACAAATTCAGGAGATGCTGCTGGGGCAGCAGGTGATGGACTGTCATCAATGCCAGGAAATGCTAGTTTACTAGGATGGGCAATGAGCTCCTTAACTCTCAAGGGTAAACCAGCTGAACAAGCTCCACTTGCACTTGTCAATACCAGCGCATCTCTTACTAAAATGACTTCCAATGACAGCTCAGCAATGGATACTCCAACCACAGCACCCGCCCATGTAAGTTCGACAACAGATTTCCCAGATCAACATGTACCCGAGTCCCCTACGTCCACAGATGGCTGGGGAGAGCTAGAAAATGGAATACAGGAAGAGCATGAAAGTGACAAGGATGGTTGGGATGATATTGAACCCCTTGAAGAGCCAACACCACCTCCAGCTCTTGCTAACATTCAAGCTGCTCAGAAGCGGCCGGTCTCGCAATCGCGGCCAAAGCAAGGTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

706

Amino Acids

77.24

Weight (kDa)

6.02

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 37 - 245 1.2e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1042, 1501, 1697
AasI GACNNNNNNGTC 1 cut(s) 1276
Acc36I ACCTGC 4 cut(s) 1042, 1378, 1501, 1697
AccB1I GGYRCC 2 cut(s) 116, 542
AccBSI CCGCTC 2 cut(s) 42, 197
AccII CGCG 1 cut(s) 2101
AclWI GGATC 4 cut(s) 40, 601, 1561, 1574
AcoI YGGCCR 4 cut(s) 181, 189, 2084, 2102
AcsI RAATTY 7 cut(s) 8, 888, 895, 943, 1013, 1082, 1681
AcuI CTGAAG 3 cut(s) 94, 734, 1098
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 5 cut(s) 223, 262, 1315, 1929, 2116
AfiI CCNNNNNNNGG 5 cut(s) 90, 501, 1323, 1499, 2111
AflIII ACRYGT 1 cut(s) 1924
AgeI ACCGGT 1 cut(s) 1232
AhdI GACNNNNNGTC 1 cut(s) 133
AjnI CCWGG 2 cut(s) 581, 1729
Alw21I GWGCWC 2 cut(s) 702, 1766
Alw26I GTCTC 5 cut(s) 141, 221, 654, 768, 2095
AlwI GGATC 4 cut(s) 40, 601, 1561, 1574
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 2 cut(s) 1497, 1931
AoxI GGCC 6 cut(s) 181, 189, 397, 660, 2084, 2102
ApoI RAATTY 7 cut(s) 8, 888, 895, 943, 1013, 1082, 1681
AseI ATTAAT 1 cut(s) 1452
AsiGI ACCGGT 1 cut(s) 1232
AspLEI GCGC 1 cut(s) 1826
AspS9I GGNCC 4 cut(s) 44, 397, 563, 660
AsuHPI GGTGA 3 cut(s) 307, 1482, 1721
AvaI CYCGRG 2 cut(s) 1497, 1931
AvaII GGWCC 2 cut(s) 44, 563
BaeGI GKGCMC 1 cut(s) 119
BalI TGGCCA 1 cut(s) 183
BamHI GGATCC 1 cut(s) 1566
BanI GGYRCC 2 cut(s) 116, 542
BanII GRGCYC 3 cut(s) 78, 702, 1766
BarI GAAGNNNNNNTAC 2 cut(s) 1652, 1684
BbsI GAAGAC 1 cut(s) 1682
Bbv12I GWGCWC 2 cut(s) 702, 1766
BccI CCATC 7 cut(s) 125, 173, 533, 1706, 1746, 1946, 2000
BceAI ACGGC 1 cut(s) 782
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 2 cut(s) 583, 1731
BciVI GTATCC 2 cut(s) 862, 1861
BclI TGATCA 2 cut(s) 1168, 1639
BcoDI GTCTC 5 cut(s) 141, 221, 654, 768, 2095
BfaI CTAG 4 cut(s) 1563, 1740, 1748, 1967
BfmI CTRYAG 1 cut(s) 1649
BfuAI ACCTGC 4 cut(s) 1042, 1378, 1501, 1697
BfuI GTATCC 2 cut(s) 862, 1861
BglII AGATCT 1 cut(s) 52
BlpI GCTNAGC 1 cut(s) 1858
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 583, 1731
Bme18I GGWCC 2 cut(s) 44, 563
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 1497, 1931
BmgT120I GGNCC 4 cut(s) 44, 397, 563, 660
BmiI GGNNCC 6 cut(s) 46, 118, 398, 544, 788, 1568
BmrFI CCNGG 2 cut(s) 583, 1731
BmsI GCATC 3 cut(s) 271, 1681, 1835
BpiI GAAGAC 1 cut(s) 1682
BplI GAGNNNNNCTC 2 cut(s) 689, 721
BpmI CTGGAG 1 cut(s) 2034
Bpu10I CCTNAGC 1 cut(s) 174
Bpu1102I GCTNAGC 1 cut(s) 1858
BpuEI CTTGAG 1 cut(s) 1760
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 2 cut(s) 141, 2095
BsaJI CCNNGG 5 cut(s) 123, 276, 495, 656, 1498
BsaWI WCCGGW 2 cut(s) 139, 1232
BsaXI ACNNNNNCTCC 2 cut(s) 2032, 2062
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 501, 1323, 1499, 2111
Bse118I RCCGGY 2 cut(s) 1232, 2086
Bse1I ACTGG 3 cut(s) 453, 474, 506
Bse3DI GCAATG 4 cut(s) 208, 1407, 1764, 1869
BseBI CCWGG 2 cut(s) 583, 1731
BseDI CCNNGG 5 cut(s) 123, 276, 495, 656, 1498
BseGI GGATG 8 cut(s) 184, 232, 286, 369, 385, 1757, 2011, 2020
BseLI CCNNNNNNNGG 5 cut(s) 90, 501, 1323, 1499, 2111
BseMI GCAATG 4 cut(s) 208, 1407, 1764, 1869
BseMII CTCAG 6 cut(s) 188, 330, 782, 1542, 1872, 2090
BseNI ACTGG 3 cut(s) 453, 474, 506
BseRI GAGGAG 2 cut(s) 746, 1515
BseSI GKGCMC 1 cut(s) 119
BseX3I CGGCCG 1 cut(s) 2084
BseYI CCCAGC 2 cut(s) 1697, 1956
BsgI GTGCAG 2 cut(s) 386, 1014
Bsh1236I CGCG 1 cut(s) 2101
Bsh1285I CGRYCG 2 cut(s) 1619, 2087
BshFI GGCC 6 cut(s) 183, 191, 399, 662, 2086, 2104
BshNI GGYRCC 2 cut(s) 116, 542
BshTI ACCGGT 1 cut(s) 1232
BsiEI CGRYCG 2 cut(s) 1619, 2087
BsiHKAI GWGCWC 2 cut(s) 702, 1766
BsiHKCI CYCGRG 2 cut(s) 1497, 1931
BsiSI CCGG 3 cut(s) 140, 1233, 2087
BslFI GGGAC 2 cut(s) 1444, 1921
BslI CCNNNNNNNGG 5 cut(s) 90, 501, 1323, 1499, 2111
BsmAI GTCTC 5 cut(s) 141, 221, 654, 768, 2095
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 1444, 1921
BsnI GGCC 6 cut(s) 183, 191, 399, 662, 2086, 2104
Bso31I GGTCTC 2 cut(s) 141, 2095
BsoBI CYCGRG 2 cut(s) 1497, 1931
Bsp1286I GDGCHC 4 cut(s) 78, 119, 702, 1766
Bsp143I GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
Bsp1720I GCTNAGC 1 cut(s) 1858
Bsp19I CCATGG 2 cut(s) 276, 656
BspANI GGCC 6 cut(s) 183, 191, 399, 662, 2086, 2104
BspCNI CTCAG 6 cut(s) 187, 331, 783, 1543, 1871, 2089
BspFNI CGCG 1 cut(s) 2101
BspLI GGNNCC 6 cut(s) 46, 118, 398, 544, 788, 1568
BspMI ACCTGC 4 cut(s) 1042, 1378, 1501, 1697
BspPI GGATC 4 cut(s) 40, 601, 1561, 1574
BspQI GCTCTTC 3 cut(s) 707, 1980, 2028
BspT107I GGYRCC 2 cut(s) 116, 542
BspTNI GGTCTC 2 cut(s) 141, 2095
BsrBI CCGCTC 2 cut(s) 42, 197
BsrDI GCAATG 4 cut(s) 208, 1407, 1764, 1869
BsrFI RCCGGY 2 cut(s) 1232, 2086
BsrI ACTGG 3 cut(s) 453, 474, 506
BssAI RCCGGY 2 cut(s) 1232, 2086
BssECI CCNNGG 5 cut(s) 123, 276, 495, 656, 1498
BssMI GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 4 cut(s) 123, 276, 495, 656
Bst2UI CCWGG 2 cut(s) 583, 1731
Bst4CI ACNGT 6 cut(s) 226, 260, 842, 1119, 1465, 1719
Bst6I CTCTTC 3 cut(s) 707, 1980, 2028
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 6 cut(s) 74, 199, 401, 513, 935, 1414
BstDEI CTNAG 6 cut(s) 174, 339, 791, 1551, 1858, 2076
BstDSI CCRYGG 2 cut(s) 276, 656
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 8 cut(s) 184, 232, 286, 369, 385, 1757, 2011, 2020
BstFNI CGCG 1 cut(s) 2101
BstHHI GCGC 1 cut(s) 1826
BstKTI GATC 9 cut(s) 35, 55, 348, 596, 1171, 1569, 1619, 1642, 1921
BstMAI GTCTC 5 cut(s) 141, 221, 654, 768, 2095
BstMBI GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
BstMCI CGRYCG 2 cut(s) 1619, 2087
BstNI CCWGG 2 cut(s) 583, 1731
BstNSI RCATGY 2 cut(s) 515, 1928
BstSCI CCNGG 2 cut(s) 581, 1729
BstSFI CTRYAG 1 cut(s) 1649
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 1 cut(s) 2101
BstV2I GAAGAC 1 cut(s) 1682
BstX2I RGATCY 3 cut(s) 32, 52, 1566
BstYI RGATCY 3 cut(s) 32, 52, 1566
BstZI CGGCCG 1 cut(s) 2084
BsuI GTATCC 2 cut(s) 862, 1861
BsuRI GGCC 6 cut(s) 183, 191, 399, 662, 2086, 2104
BtgI CCRYGG 2 cut(s) 276, 656
BtsCI GGATG 8 cut(s) 184, 232, 286, 369, 385, 1757, 2011, 2020
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 4 cut(s) 481, 744, 847, 1548
BveI ACCTGC 4 cut(s) 1042, 1378, 1501, 1697
Cac8I GCNNGC 6 cut(s) 74, 199, 401, 513, 935, 1414
CaiI CAGNNNCTG 1 cut(s) 1028
CfoI GCGC 1 cut(s) 1826
Cfr10I RCCGGY 2 cut(s) 1232, 2086
Cfr13I GGNCC 4 cut(s) 44, 397, 563, 660
CseI GACGC 2 cut(s) 196, 203
Csp6I GTAC 5 cut(s) 222, 261, 1314, 1928, 2115
CspAI ACCGGT 1 cut(s) 1232
CviAII CATG 9 cut(s) 277, 455, 512, 657, 883, 1892, 1925, 1991, 2118
CviQI GTAC 5 cut(s) 222, 261, 1314, 1928, 2115
DdeI CTNAG 6 cut(s) 174, 339, 791, 1551, 1858, 2076
DpnI GATC 9 cut(s) 34, 54, 347, 595, 1170, 1568, 1618, 1641, 1920
DpnII GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
DrdI GACNNNNNNGTC 1 cut(s) 1276
DriI GACNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 1276
EaeI YGGCCR 4 cut(s) 181, 189, 2084, 2102
EagI CGGCCG 1 cut(s) 2084
Eam1104I CTCTTC 3 cut(s) 707, 1980, 2028
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 3 cut(s) 707, 1980, 2028
Ecl136II GAGCTC 2 cut(s) 700, 1764
EclXI CGGCCG 1 cut(s) 2084
Eco130I CCWWGG 4 cut(s) 123, 276, 495, 656
Eco24I GRGCYC 3 cut(s) 78, 702, 1766
Eco31I GGTCTC 2 cut(s) 141, 2095
Eco47I GGWCC 2 cut(s) 44, 563
Eco52I CGGCCG 1 cut(s) 2084
Eco53kI GAGCTC 2 cut(s) 700, 1764
Eco57I CTGAAG 3 cut(s) 94, 734, 1098
Eco88I CYCGRG 2 cut(s) 1497, 1931
EcoICRI GAGCTC 2 cut(s) 700, 1764
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 581, 1729
EcoT14I CCWWGG 4 cut(s) 123, 276, 495, 656
EcoT22I ATGCAT 1 cut(s) 1459
EcoT38I GRGCYC 3 cut(s) 78, 702, 1766
ErhI CCWWGG 4 cut(s) 123, 276, 495, 656
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 9 cut(s) 280, 458, 515, 660, 886, 1895, 1928, 1994, 2121
FalI AAGNNNNNCTT 2 cut(s) 1788, 1820
FaqI GGGAC 2 cut(s) 1444, 1921
FatI CATG 9 cut(s) 276, 454, 511, 656, 882, 1891, 1924, 1990, 2117
FauI CCCGC 1 cut(s) 1894
FbaI TGATCA 2 cut(s) 1168, 1639
FokI GGATG 8 cut(s) 191, 219, 293, 356, 392, 1764, 2018, 2027
FriOI GRGCYC 3 cut(s) 78, 702, 1766
FspBI CTAG 4 cut(s) 1563, 1740, 1748, 1967
GlaI GCGC 1 cut(s) 1825
GsaI CCCAGC 2 cut(s) 1701, 1960
GsuI CTGGAG 1 cut(s) 2034
HaeIII GGCC 6 cut(s) 183, 191, 399, 662, 2086, 2104
HapII CCGG 3 cut(s) 140, 1233, 2087
HgaI GACGC 2 cut(s) 196, 203
HhaI GCGC 1 cut(s) 1826
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 9 cut(s) 280, 458, 515, 660, 886, 1895, 1928, 1994, 2121
Hin6I GCGC 1 cut(s) 1824
HinP1I GCGC 1 cut(s) 1824
HindIII AAGCTT 3 cut(s) 827, 935, 1301
HinfI GANTC 5 cut(s) 16, 668, 1181, 1270, 1934
HpaII CCGG 3 cut(s) 140, 1233, 2087
HphI GGTGA 3 cut(s) 307, 1482, 1721
Hpy166II GTNNAC 5 cut(s) 362, 446, 1745, 1784, 1947
Hpy188I TCNGA 9 cut(s) 230, 340, 532, 628, 644, 714, 942, 1243, 2079
Hpy188III TCNNGA 6 cut(s) 13, 176, 773, 790, 1262, 1686
Hpy8I GTNNAC 5 cut(s) 362, 446, 1745, 1784, 1947
Hpy99I CGWCG 1 cut(s) 826
HpyAV CCTTC 5 cut(s) 813, 895, 1087, 1421, 1615
HpyCH4III ACNGT 6 cut(s) 226, 260, 842, 1119, 1465, 1719
HpyCH4IV ACGT 4 cut(s) 821, 1224, 1312, 1943
HpyF3I CTNAG 6 cut(s) 174, 339, 791, 1551, 1858, 2076
HpySE526I ACGT 4 cut(s) 821, 1224, 1312, 1943
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 9 cut(s) 280, 458, 515, 660, 886, 1895, 1928, 1994, 2121
HspAI GCGC 1 cut(s) 1824
Ksp22I TGATCA 2 cut(s) 1168, 1639
Kzo9I GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
LguI GCTCTTC 3 cut(s) 707, 1980, 2028
LmnI GCTCC 7 cut(s) 39, 733, 792, 1300, 1502, 1769, 1804
LweI GCATC 3 cut(s) 271, 1681, 1835
MaeI CTAG 4 cut(s) 1563, 1740, 1748, 1967
MaeII ACGT 4 cut(s) 821, 1224, 1312, 1943
MaeIII GTNAC 5 cut(s) 313, 484, 984, 1350, 1997
MalI GATC 9 cut(s) 34, 54, 347, 595, 1170, 1568, 1618, 1641, 1920
MbiI CCGCTC 2 cut(s) 42, 197
MboI GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
MboII GAAGA 9 cut(s) 355, 694, 919, 1091, 1250, 1376, 1687, 1997, 2045
MflI RGATCY 3 cut(s) 32, 52, 1566
MhlI GDGCHC 4 cut(s) 78, 119, 702, 1766
MlsI TGGCCA 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 2 cut(s) 1264, 1943
MmeI TCCRAC 2 cut(s) 571, 1898
MnlI CCTC 8 cut(s) 94, 130, 544, 724, 972, 1493, 1580, 2058
Mox20I TGGCCA 1 cut(s) 183
Mph1103I ATGCAT 1 cut(s) 1459
MscI TGGCCA 1 cut(s) 183
MseI TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
MslI CAYNNNNRTG 3 cut(s) 459, 1422, 1995
Msp20I TGGCCA 1 cut(s) 183
MspA1I CMGCKG 3 cut(s) 784, 1025, 1790
MspI CCGG 3 cut(s) 140, 1233, 2087
MspR9I CCNGG 2 cut(s) 583, 1731
MvaI CCWGG 2 cut(s) 583, 1731
MvnI CGCG 1 cut(s) 2101
NcoI CCATGG 2 cut(s) 276, 656
NdeII GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
NlaIII CATG 9 cut(s) 280, 458, 515, 660, 886, 1895, 1928, 1994, 2121
NlaIV GGNNCC 6 cut(s) 46, 118, 398, 544, 788, 1568
NmuCI GTSAC 2 cut(s) 313, 1997
NsiI ATGCAT 1 cut(s) 1459
NspI RCATGY 2 cut(s) 515, 1928
PaeI GCATGC 1 cut(s) 515
PaqCI CACCTGC 3 cut(s) 1042, 1501, 1697
PciI ACATGT 1 cut(s) 1924
PciSI GCTCTTC 3 cut(s) 707, 1980, 2028
PfeI GAWTC 3 cut(s) 16, 668, 1181
PinAI ACCGGT 1 cut(s) 1232
Ple19I CGATCG 1 cut(s) 1619
PleI GAGTC 2 cut(s) 1264, 1942
PpsI GAGTC 2 cut(s) 1264, 1942
PscI ACATGT 1 cut(s) 1924
PshBI ATTAAT 1 cut(s) 1452
Psp124BI GAGCTC 2 cut(s) 702, 1766
Psp6I CCWGG 2 cut(s) 581, 1729
PspFI CCCAGC 2 cut(s) 1697, 1956
PspGI CCWGG 2 cut(s) 581, 1729
PspN4I GGNNCC 6 cut(s) 46, 118, 398, 544, 788, 1568
PspPI GGNCC 4 cut(s) 44, 397, 563, 660
PsrI GAACNNNNNNTAC 4 cut(s) 345, 377, 1372, 1404
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 3 cut(s) 32, 52, 1566
PvuI CGATCG 1 cut(s) 1619
PvuII CAGCTG 2 cut(s) 1025, 1790
RsaI GTAC 5 cut(s) 223, 262, 1315, 1929, 2116
RsaNI GTAC 5 cut(s) 222, 261, 1314, 1928, 2115
RseI CAYNNNNRTG 3 cut(s) 459, 1422, 1995
SacI GAGCTC 2 cut(s) 702, 1766
SapI GCTCTTC 3 cut(s) 707, 1980, 2028
SaqAI TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
Sau3AI GATC 9 cut(s) 32, 52, 345, 593, 1168, 1566, 1616, 1639, 1918
Sau96I GGNCC 4 cut(s) 44, 397, 563, 660
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 2 cut(s) 1264, 1943
ScrFI CCNGG 2 cut(s) 583, 1731
SduI GDGCHC 4 cut(s) 78, 119, 702, 1766
SfaNI GCATC 3 cut(s) 271, 1681, 1835
SfcI CTRYAG 1 cut(s) 1649
SinI GGWCC 2 cut(s) 44, 563
SmiMI CAYNNNNRTG 3 cut(s) 459, 1422, 1995
SmlI CTYRAG 1 cut(s) 1775
SmoI CTYRAG 1 cut(s) 1775
SphI GCATGC 1 cut(s) 515
SspI AATATT 1 cut(s) 386
SspMI CTAG 4 cut(s) 1563, 1740, 1748, 1967
SstI GAGCTC 2 cut(s) 702, 1766
StyD4I CCNGG 2 cut(s) 581, 1729
StyI CCWWGG 4 cut(s) 123, 276, 495, 656
TaaI ACNGT 6 cut(s) 226, 260, 842, 1119, 1465, 1719
TaiI ACGT 4 cut(s) 824, 1227, 1315, 1946
TaqI TCGA 6 cut(s) 19, 824, 1144, 1615, 1619, 1901
TaqII GACCGA 1 cut(s) 1149
TatI WGTACW 1 cut(s) 260
TauI GCSGC 6 cut(s) 194, 197, 638, 787, 2086, 2104
TfiI GAWTC 3 cut(s) 16, 668, 1181
Tru1I TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
Tru9I TTAA 5 cut(s) 431, 719, 1052, 1452, 1769
TscAI CASTG 4 cut(s) 481, 751, 847, 1555
TseFI GTSAC 2 cut(s) 313, 1997
Tsp45I GTSAC 2 cut(s) 313, 1997
TspDTI ATGAA 6 cut(s) 396, 443, 871, 1377, 1440, 2007
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 4 cut(s) 481, 751, 847, 1555
VpaK11BI GGWCC 2 cut(s) 44, 563
VspI ATTAAT 1 cut(s) 1452
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 7 cut(s) 8, 888, 895, 943, 1013, 1082, 1681
XceI RCATGY 2 cut(s) 515, 1928
XspI CTAG 4 cut(s) 1563, 1740, 1748, 1967
ZrmI AGTACT 1 cut(s) 262
Zsp2I ATGCAT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.