pycom13g00220
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010351
Physical Location & Seq
Forward (+)
144032 .. 152329
8298 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00220.18

Sequence Viewer

Length: 2394 bp
ATGCTGAAATTCTTGAAGGGAGTGGTGGGCGGATCGGGGACTGGACCCAAAGATCTGCCCTACAACATCGGCGAACCTTATCCCTCTGCTTGGGGCTCTTGGACTCACTTCCGAGGCACCTCCAAGGACGATGGTACTCCGGTGTCCATATTCTCTATATCTGGAAGTAGTACTCAGGATGGCCATTTGGCCGCTGCTCGCAATGGCGTCAAGCGTCTCCGCACTGTCAGACACCCAAACATCTTATCATTTCTTCATAGTACTGAGGCTGAAACTGTGGATGCTTCTTCTGCCATCAAGCAGACTATCTATATCGTTACCGAGCCTGTTATGCCACTCTCTGAAAAGATTAAGGAGCTCAATTTACAAGGTATCCAAAGGGATGAGTATTTCGCATGTGGACTGAACCAGATAGCTAAGGCTGTCAGTTTCTTGAATAATGATTGTAAACTTGTTCATGCGAACGTTTGCTTGGCCAGTGTTGTTGTAACTCAAACTTTGGACTGGAAGTTACATGCTTTTGATGTGCTATCTGAGTTTGATGGCGGCAATGAGTCTTCTGCCGGACAAATGCTGCAATTCGCCTGGCTTGTTGGACCACAATACAAACCCACGGAGTTGCTGAAGTCTGATTGGGCTGCAATCAGAAAGTCTCCACCATGGGCTATTGATTCTTGGGGCTTGGGCTGTCTTATATACGAGCTCTTTTCCGGTCTGAAGTTAAGCAAAACAGAGGAACTGCGCAACACGGCTTCTATTCCAAAGTCTCTGCTTCCAGATTATCAGCGGCTCTTGAGTTCCACGCCTTCTCGTAGGTTGAATACATCGAAGCTTATAGAAAATAGTGAATATTTCCAAAATAAGCTAGTGGATACAATACATTTCATGGAAATCCTAAATCTGAAAGATAGTGTTGAGAAGGATACCTTCTTCCGCAAGCTTCCAAATTTAGTGGAGCAGCTACCTCGCCAAATTGTGCTGAAAAAGTTGCTTCCTTTGTTGGCTTCTGCCCTTGAATTTGGTTCAGCTGCTGCTCCTGCTTTGACAGCTTTGTTGAAAATGGGTTCCTGGCTTTCAACCGAAGAATTCAGTGTGAAGGTGCTGCCAACAATTGTGAAACTGTTTGCTTCCAATGACCGAGCTATTCGAGTTGGACTTTTGCAACATATCGATCAGTTTGGAGAATCATTATCAGCACAAATTGTTGATGAGCAAGTTTACCCCCACGTTGCTACGGGGTTCACTGACACATCTGCTTTTCTCCGGGAACTGACTTTGAAGTCGATGCTTGTTCTAGCTCCTAAGCTTTCTCAACGCACCATTTCAGGGTCCTTGTTGAAGTATCTTTCAAAGTTACAGGTTGATGAAGAACCAGCAATTAGAACGAACACCACTATATTACTAGGGAACATTGCATCCCACCTGAACGACGGGACAAGGAAAAGAGTGCTGATTAATGCTTTTACTGCCCGTGCATTACGTGATACTTTCTCACCTGCCCGGGGAGCAGGTATTATGGCTTTGTGTGCTACCAGTTCTTATTATGATATCACTGAGATTGCAACTCGGATTCTCCCAAATGTTGTTATTCTTACCATTGATCCGGATAATGATGTTCGATCAAAGGCATTTCAAGCAGTCGATCAATTTTTACAAATAGTGAAGCAATCCTATGAAAAGACTGATTCAGGAGATACTGCCGGTGTTGGAATCTCAGCACTACCAGGAAATGCTAGTTTACTGGGATGGGCAATGAGCTCCTTGACTCTCAAGGGTAAACCTTCTGAACAAGCTCCACTTGCTCCTGTGAATACCAGTGCATCTCTTACTAAAACAACTTCTAATGCAAGCTCAGTGGTGGATACTCCAAGCACAGTGCCGGCTCACGTAAGTTCGACAACAGATTTTGCCGACCACCATATACCTGAGTCCCCAACGTCTACTGATGGCTGGGGAGAACTTGAAAATGGAATTCACGAGGAGAATGAAAGTGACAAGGATGGGTGGGACGATGTTGAACCCCTGGAAGAGCCAAAACCGTCTCCTGTTCTTGCGAACATTCAAGCAGCTCAAAAGCGGCCGGTGTCACAACATGTTTCGCAGCCTAAACAAGCAACGAGTTTGAGACCAAAAAACACAGCCAAGGCTGTCAAAAATGAAGATGATGATTTGTGGGGATCCATTGCCGCCCCTGCACCAAAAACAGTTTCAAAACCTTTGAATGTGAAAGCAAGTGCAGCAGTTGATGATGATGATCCATGGGCTGCGATTGCCGCTCCCCCACCCTCTACCAAGGCAAAGCCTCTATCAGTAGCCAAAGGCCGAGGAGCCAAAGCAGCTGCTCCAAAATTGGGCGCACAGAGGATAAACCGGACGTCTTCGTCGGGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

798

Amino Acids

86.27

Weight (kDa)

6.96

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1504
AasI GACNNNNNNGTC 2 cut(s) 1279, 2380
AatII GACGTC 1 cut(s) 2378
Acc16I TGCGCA 1 cut(s) 743
Acc36I ACCTGC 2 cut(s) 1499, 1504
AccB1I GGYRCC 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 2276
AccI GTMKAC 1 cut(s) 1940
AccIII TCCGGA 1 cut(s) 1603
AciI CCGC 9 cut(s) 30, 192, 220, 546, 787, 934, 2077, 2187, 2274
AclI AACGTT 1 cut(s) 465
AclWI GGATC 5 cut(s) 40, 1595, 2172, 2185, 2249
AcoI YGGCCR 4 cut(s) 181, 189, 474, 2078
AcsI RAATTY 5 cut(s) 8, 946, 1016, 1085, 1971
AcuI CTGAAG 2 cut(s) 644, 737
AcyI GRCGYC 2 cut(s) 207, 2375
AfaI GTAC 3 cut(s) 136, 172, 262
AfiI CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
AflIII ACRYGT 1 cut(s) 2092
AjnI CCWGG 4 cut(s) 584, 1067, 1723, 2022
AjuI GAANNNNNNNTTGG 4 cut(s) 455, 487, 2191, 2223
AloI GAACNNNNNNTCC 2 cut(s) 1400, 1432
Alw21I GWGCWC 3 cut(s) 360, 705, 1760
Alw26I GTCTC 5 cut(s) 221, 657, 771, 2046, 2119
AlwI GGATC 5 cut(s) 40, 1595, 2172, 2185, 2249
AlwNI CAGNNNCTG 1 cut(s) 1031
Ama87I CYCGRG 1 cut(s) 1500
Aor13HI TCCGGA 1 cut(s) 1603
AoxI GGCC 5 cut(s) 181, 189, 474, 2078, 2320
ApoI RAATTY 5 cut(s) 8, 946, 1016, 1085, 1971
AseI ATTAAT 1 cut(s) 1455
AspLEI GCGC 2 cut(s) 744, 2357
AspS9I GGNCC 3 cut(s) 44, 596, 1329
AsuC2I CCSGG 3 cut(s) 1265, 1501, 1502
AsuHPI GGTGA 1 cut(s) 1485
AvaI CYCGRG 1 cut(s) 1500
AvaII GGWCC 3 cut(s) 44, 596, 1329
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BalI TGGCCA 2 cut(s) 183, 476
BamHI GGATCC 1 cut(s) 2177
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 4 cut(s) 98, 360, 705, 1760
BauI CACGAG 1 cut(s) 1976
BbsI GAAGAC 2 cut(s) 549, 2370
Bbv12I GWGCWC 3 cut(s) 360, 705, 1760
BccI CCATC 7 cut(s) 125, 173, 302, 536, 1740, 1940, 1994
BceAI ACGGC 1 cut(s) 765
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 4 cut(s) 586, 1069, 1725, 2024
BciVI GTATCC 4 cut(s) 383, 865, 916, 1855
BcnI CCSGG 3 cut(s) 1265, 1501, 1502
BcoDI GTCTC 5 cut(s) 221, 657, 771, 2046, 2119
BfaI CTAG 4 cut(s) 866, 1295, 1403, 1734
BfuAI ACCTGC 2 cut(s) 1499, 1504
BfuI GTATCC 4 cut(s) 383, 865, 916, 1855
BglII AGATCT 1 cut(s) 52
BmcAI AGTACT 2 cut(s) 172, 262
Bme1390I CCNGG 7 cut(s) 586, 1069, 1265, 1501, 1502, 1725, 2024
Bme18I GGWCC 3 cut(s) 44, 596, 1329
BmeT110I CYCGRG 1 cut(s) 1500
BmgT120I GGNCC 3 cut(s) 44, 596, 1329
BmiI GGNNCC 6 cut(s) 46, 118, 1066, 1330, 2179, 2329
BmrFI CCNGG 7 cut(s) 586, 1069, 1265, 1501, 1502, 1725, 2024
BmrI ACTGGG 1 cut(s) 1751
BmsI GCATC 4 cut(s) 271, 1275, 1424, 1829
BmuI ACTGGG 1 cut(s) 1751
BpiI GAAGAC 2 cut(s) 549, 2370
Bpu10I CCTNAGC 2 cut(s) 417, 1302
BpuEI CTTGAG 2 cut(s) 814, 1754
BpuMI CCSGG 3 cut(s) 1265, 1501, 1502
Bsa29I ATCGAT 1 cut(s) 1170
BsaAI YACGTR 2 cut(s) 1481, 1888
BsaBI GATNNNNATC 1 cut(s) 2253
BsaHI GRCGYC 2 cut(s) 207, 2375
BsaI GGTCTC 1 cut(s) 2119
BsaWI WCCGGW 4 cut(s) 139, 710, 1603, 2370
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
Bse118I RCCGGY 3 cut(s) 1700, 1879, 2080
Bse1I ACTGG 6 cut(s) 46, 477, 509, 1533, 1746, 1815
Bse3DI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
Bse8I GATNNNNATC 1 cut(s) 2253
BseAI TCCGGA 1 cut(s) 1603
BseBI CCWGG 4 cut(s) 586, 1069, 1725, 2024
BseCI ATCGAT 1 cut(s) 1170
BseGI GGATG 6 cut(s) 184, 286, 388, 1415, 1751, 2005
BseJI GATNNNNATC 1 cut(s) 2253
BseLI CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
BseMI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
BseMII CTCAG 7 cut(s) 188, 255, 525, 1545, 1728, 1866, 1917
BseNI ACTGG 6 cut(s) 46, 477, 509, 1533, 1746, 1815
BseRI GAGGAG 2 cut(s) 1994, 2340
BseX3I CGGCCG 1 cut(s) 2078
BseYI CCCAGC 1 cut(s) 1950
BsgI GTGCAG 2 cut(s) 2178, 2256
Bsh1285I CGRYCG 1 cut(s) 2081
BshFI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
BshNI GGYRCC 1 cut(s) 116
BshVI ATCGAT 1 cut(s) 1170
BsiEI CGRYCG 1 cut(s) 2081
BsiHKAI GWGCWC 3 cut(s) 360, 705, 1760
BsiHKCI CYCGRG 1 cut(s) 1500
BslFI GGGAC 4 cut(s) 52, 1447, 1915, 2021
BslI CCNNNNNNNGG 4 cut(s) 90, 1326, 1502, 2351
BsmAI GTCTC 5 cut(s) 221, 657, 771, 2046, 2119
BsmBI CGTCTC 2 cut(s) 221, 2046
BsmFI GGGAC 4 cut(s) 52, 1447, 1915, 2021
BsnI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
Bso31I GGTCTC 1 cut(s) 2119
BsoBI CYCGRG 1 cut(s) 1500
Bsp1286I GDGCHC 4 cut(s) 98, 360, 705, 1760
Bsp13I TCCGGA 1 cut(s) 1603
Bsp143I GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
Bsp19I CCATGG 2 cut(s) 659, 2258
BspACI CCGC 9 cut(s) 30, 192, 220, 546, 787, 934, 2077, 2187, 2274
BspANI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
BspCNI CTCAG 7 cut(s) 187, 256, 526, 1546, 1727, 1865, 1918
BspDI ATCGAT 1 cut(s) 1170
BspEI TCCGGA 1 cut(s) 1603
BspLI GGNNCC 6 cut(s) 46, 118, 1066, 1330, 2179, 2329
BspMI ACCTGC 2 cut(s) 1499, 1504
BspPI GGATC 5 cut(s) 40, 1595, 2172, 2185, 2249
BspQI GCTCTTC 1 cut(s) 2022
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 2119
BsrBI CCGCTC 1 cut(s) 2276
BsrDI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
BsrFI RCCGGY 3 cut(s) 1700, 1879, 2080
BsrI ACTGG 6 cut(s) 46, 477, 509, 1533, 1746, 1815
BssAI RCCGGY 3 cut(s) 1700, 1879, 2080
BssMI GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
BssNI GRCGYC 2 cut(s) 207, 2375
BssSI CACGAG 1 cut(s) 1976
BssT1I CCWWGG 5 cut(s) 123, 659, 2142, 2258, 2292
Bst2BI CACGAG 1 cut(s) 1976
Bst2UI CCWGG 4 cut(s) 586, 1069, 1725, 2024
Bst4CI ACNGT 6 cut(s) 226, 277, 1122, 1876, 2040, 2206
Bst6I CTCTTC 1 cut(s) 2022
BstACI GRCGYC 2 cut(s) 207, 2375
BstBAI YACGTR 2 cut(s) 1481, 1888
BstC8I GCNNGC 4 cut(s) 199, 938, 1849, 1881
BstDEI CTNAG 9 cut(s) 174, 264, 417, 534, 1302, 1554, 1714, 1852, 1926
BstDSI CCRYGG 3 cut(s) 612, 659, 2258
BstF5I GGATG 6 cut(s) 184, 286, 388, 1415, 1751, 2005
BstHHI GCGC 2 cut(s) 744, 2357
BstKTI GATC 8 cut(s) 35, 55, 1174, 1603, 1622, 1645, 2180, 2257
BstMAI GTCTC 5 cut(s) 221, 657, 771, 2046, 2119
BstMBI GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
BstMCI CGRYCG 1 cut(s) 2081
BstNI CCWGG 4 cut(s) 586, 1069, 1725, 2024
BstNSI RCATGY 3 cut(s) 399, 518, 2096
BstSCI CCNGG 7 cut(s) 584, 1067, 1263, 1499, 1500, 1723, 2022
BstV2I GAAGAC 2 cut(s) 549, 2370
BstX2I RGATCY 2 cut(s) 52, 2177
BstYI RGATCY 2 cut(s) 52, 2177
BstZI CGGCCG 1 cut(s) 2078
Bsu15I ATCGAT 1 cut(s) 1170
BsuI GTATCC 4 cut(s) 383, 865, 916, 1855
BsuRI GGCC 5 cut(s) 183, 191, 476, 2080, 2322
BsuTUI ATCGAT 1 cut(s) 1170
BtgI CCRYGG 3 cut(s) 612, 659, 2258
BtsCI GGATG 6 cut(s) 184, 286, 388, 1415, 1751, 2005
BtsIMutI CAGTG 8 cut(s) 222, 484, 1096, 1242, 1551, 1822, 1860, 1881
BveI ACCTGC 2 cut(s) 1499, 1504
Cac8I GCNNGC 4 cut(s) 199, 938, 1849, 1881
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 2 cut(s) 744, 2357
Cfr10I RCCGGY 3 cut(s) 1700, 1879, 2080
Cfr13I GGNCC 3 cut(s) 44, 596, 1329
Cfr9I CCCGGG 1 cut(s) 1500
ClaI ATCGAT 1 cut(s) 1170
CseI GACGC 2 cut(s) 196, 203
Csp6I GTAC 3 cut(s) 135, 171, 261
CspCI CAANNNNNGTGG 4 cut(s) 933, 968, 1836, 1871
CviAII CATG 7 cut(s) 396, 458, 515, 660, 886, 2093, 2259
CviQI GTAC 3 cut(s) 135, 171, 261
DdeI CTNAG 9 cut(s) 174, 264, 417, 534, 1302, 1554, 1714, 1852, 1926
DpnI GATC 8 cut(s) 34, 54, 1173, 1602, 1621, 1644, 2179, 2256
DpnII GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
DrdI GACNNNNNNGTC 2 cut(s) 1279, 2380
DseDI GACNNNNNNGTC 2 cut(s) 1279, 2380
EaeI YGGCCR 4 cut(s) 181, 189, 474, 2078
EagI CGGCCG 1 cut(s) 2078
Eam1104I CTCTTC 1 cut(s) 2022
EarI CTCTTC 1 cut(s) 2022
EciI GGCGGA 1 cut(s) 45
Ecl136II GAGCTC 3 cut(s) 358, 703, 1758
EclXI CGGCCG 1 cut(s) 2078
Eco130I CCWWGG 5 cut(s) 123, 659, 2142, 2258, 2292
Eco24I GRGCYC 4 cut(s) 98, 360, 705, 1760
Eco31I GGTCTC 1 cut(s) 2119
Eco32I GATATC 1 cut(s) 1549
Eco47I GGWCC 3 cut(s) 44, 596, 1329
Eco52I CGGCCG 1 cut(s) 2078
Eco53kI GAGCTC 3 cut(s) 358, 703, 1758
Eco57I CTGAAG 2 cut(s) 644, 737
Eco88I CYCGRG 1 cut(s) 1500
EcoICRI GAGCTC 3 cut(s) 358, 703, 1758
EcoO109I RGGNCCY 1 cut(s) 1329
EcoRI GAATTC 2 cut(s) 1085, 1971
EcoRII CCWGG 4 cut(s) 584, 1067, 1723, 2022
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 5 cut(s) 123, 659, 2142, 2258, 2292
EcoT38I GRGCYC 4 cut(s) 98, 360, 705, 1760
ErhI CCWWGG 5 cut(s) 123, 659, 2142, 2258, 2292
Esp3I CGTCTC 2 cut(s) 221, 2046
FaeI CATG 7 cut(s) 399, 461, 518, 663, 889, 2096, 2262
FalI AAGNNNNNCTT 4 cut(s) 911, 943, 1782, 1814
FaqI GGGAC 4 cut(s) 52, 1447, 1915, 2021
FatI CATG 7 cut(s) 395, 457, 514, 659, 885, 2092, 2258
FblI GTMKAC 1 cut(s) 1940
FokI GGATG 6 cut(s) 191, 293, 395, 1402, 1758, 2012
FriOI GRGCYC 4 cut(s) 98, 360, 705, 1760
FspBI CTAG 4 cut(s) 866, 1295, 1403, 1734
FspI TGCGCA 1 cut(s) 743
GlaI GCGC 2 cut(s) 743, 2356
GsaI CCCAGC 1 cut(s) 1954
HaeIII GGCC 5 cut(s) 183, 191, 476, 2080, 2322
HgaI GACGC 2 cut(s) 196, 203
HhaI GCGC 2 cut(s) 744, 2357
Hin1I GRCGYC 2 cut(s) 207, 2375
Hin1II CATG 7 cut(s) 399, 461, 518, 663, 889, 2096, 2262
Hin6I GCGC 2 cut(s) 742, 2355
HinP1I GCGC 2 cut(s) 742, 2355
HindIII AAGCTT 3 cut(s) 830, 938, 1304
HinfI GANTC 9 cut(s) 103, 554, 671, 1184, 1570, 1685, 1710, 1765, 1928
HphI GGTGA 1 cut(s) 1485
Hpy166II GTNNAC 7 cut(s) 401, 449, 1219, 1242, 1739, 1778, 1941
Hpy188III TCNNGA 9 cut(s) 13, 162, 176, 433, 776, 793, 1604, 1689, 1976
Hpy8I GTNNAC 7 cut(s) 401, 449, 1219, 1242, 1739, 1778, 1941
Hpy99I CGWCG 2 cut(s) 1433, 2386
HpyAV CCTTC 6 cut(s) 10, 816, 913, 937, 1090, 1791
HpyCH4III ACNGT 6 cut(s) 226, 277, 1122, 1876, 2040, 2206
HpyCH4IV ACGT 6 cut(s) 465, 1227, 1480, 1887, 1937, 2375
HpyF3I CTNAG 9 cut(s) 174, 264, 417, 534, 1302, 1554, 1714, 1852, 1926
HpySE526I ACGT 6 cut(s) 465, 1227, 1480, 1887, 1937, 2375
Hsp92I GRCGYC 2 cut(s) 207, 2375
Hsp92II CATG 7 cut(s) 399, 461, 518, 663, 889, 2096, 2262
HspAI GCGC 2 cut(s) 742, 2355
Kpn2I TCCGGA 1 cut(s) 1603
KroI GCCGGC 1 cut(s) 1879
KroNI GCCGGC 1 cut(s) 1881
Kzo9I GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
LguI GCTCTTC 1 cut(s) 2022
LweI GCATC 4 cut(s) 271, 1275, 1424, 1829
MaeI CTAG 4 cut(s) 866, 1295, 1403, 1734
MaeII ACGT 6 cut(s) 465, 1227, 1480, 1887, 1937, 2375
MaeIII GTNAC 6 cut(s) 316, 487, 510, 1353, 1991, 2085
MalI GATC 8 cut(s) 34, 54, 1173, 1602, 1621, 1644, 2179, 2256
MbiI CCGCTC 1 cut(s) 2276
MboI GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
MboII GAAGA 9 cut(s) 245, 279, 549, 922, 1094, 1379, 2039, 2171, 2370
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 2 cut(s) 52, 2177
MhlI GDGCHC 4 cut(s) 98, 360, 705, 1760
MlsI TGGCCA 2 cut(s) 183, 476
MluNI TGGCCA 2 cut(s) 183, 476
MlyI GAGTC 4 cut(s) 97, 563, 1759, 1937
MmeI TCCRAC 3 cut(s) 574, 1132, 1687
Mox20I TGGCCA 2 cut(s) 183, 476
MroI TCCGGA 1 cut(s) 1603
MroNI GCCGGC 1 cut(s) 1879
MscI TGGCCA 2 cut(s) 183, 476
MseI TTAA 3 cut(s) 351, 722, 1455
Msp20I TGGCCA 2 cut(s) 183, 476
MspA1I CMGCKG 4 cut(s) 194, 787, 1028, 2339
MspR9I CCNGG 7 cut(s) 586, 1069, 1265, 1501, 1502, 1725, 2024
MunI CAATTG 1 cut(s) 1110
MvaI CCWGG 4 cut(s) 586, 1069, 1725, 2024
NaeI GCCGGC 1 cut(s) 1881
NciI CCSGG 3 cut(s) 1265, 1501, 1502
NcoI CCATGG 2 cut(s) 659, 2258
NdeII GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
NgoMIV GCCGGC 1 cut(s) 1879
NlaIII CATG 7 cut(s) 399, 461, 518, 663, 889, 2096, 2262
NlaIV GGNNCC 6 cut(s) 46, 118, 1066, 1330, 2179, 2329
NmeAIII GCCGAG 1 cut(s) 2348
NmuCI GTSAC 2 cut(s) 1991, 2085
NsbI TGCGCA 1 cut(s) 743
NspI RCATGY 3 cut(s) 399, 518, 2096
PaqCI CACCTGC 1 cut(s) 1504
PciI ACATGT 1 cut(s) 2092
PciSI GCTCTTC 1 cut(s) 2022
PdiI GCCGGC 1 cut(s) 1881
PfeI GAWTC 5 cut(s) 671, 1184, 1570, 1685, 1710
PfoI TCCNGGA 1 cut(s) 1263
PleI GAGTC 4 cut(s) 97, 562, 1759, 1936
PpsI GAGTC 4 cut(s) 97, 562, 1759, 1936
Ppu21I YACGTR 2 cut(s) 1481, 1888
PpuMI RGGWCCY 1 cut(s) 1329
PscI ACATGT 1 cut(s) 2092
PshBI ATTAAT 1 cut(s) 1455
Psp124BI GAGCTC 3 cut(s) 360, 705, 1760
Psp1406I AACGTT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 1329
Psp6I CCWGG 4 cut(s) 584, 1067, 1723, 2022
PspFI CCCAGC 1 cut(s) 1950
PspGI CCWGG 4 cut(s) 584, 1067, 1723, 2022
PspN4I GGNNCC 6 cut(s) 46, 118, 1066, 1330, 2179, 2329
PspPI GGNCC 3 cut(s) 44, 596, 1329
PspPPI RGGWCCY 1 cut(s) 1329
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 2 cut(s) 52, 2177
PvuII CAGCTG 2 cut(s) 1028, 2339
RsaI GTAC 3 cut(s) 136, 172, 262
RsaNI GTAC 3 cut(s) 135, 171, 261
SacI GAGCTC 3 cut(s) 360, 705, 1760
SapI GCTCTTC 1 cut(s) 2022
SaqAI TTAA 3 cut(s) 351, 722, 1455
Sau3AI GATC 8 cut(s) 32, 52, 1171, 1600, 1619, 1642, 2177, 2254
Sau96I GGNCC 3 cut(s) 44, 596, 1329
ScaI AGTACT 2 cut(s) 172, 262
SchI GAGTC 4 cut(s) 97, 563, 1759, 1937
ScrFI CCNGG 7 cut(s) 586, 1069, 1265, 1501, 1502, 1725, 2024
SduI GDGCHC 4 cut(s) 98, 360, 705, 1760
SfaNI GCATC 4 cut(s) 271, 1275, 1424, 1829
SinI GGWCC 3 cut(s) 44, 596, 1329
SmaI CCCGGG 1 cut(s) 1502
SmlI CTYRAG 2 cut(s) 793, 1769
SmoI CTYRAG 2 cut(s) 793, 1769
SsiI CCGC 9 cut(s) 30, 192, 220, 546, 787, 934, 2077, 2187, 2274
SspI AATATT 1 cut(s) 851
SspMI CTAG 4 cut(s) 866, 1295, 1403, 1734
SstI GAGCTC 3 cut(s) 360, 705, 1760
StyD4I CCNGG 7 cut(s) 584, 1067, 1263, 1499, 1500, 1723, 2022
StyI CCWWGG 5 cut(s) 123, 659, 2142, 2258, 2292
TaaI ACNGT 6 cut(s) 226, 277, 1122, 1876, 2040, 2206
TaiI ACGT 6 cut(s) 468, 1230, 1483, 1890, 1940, 2378
TaqI TCGA 7 cut(s) 827, 1147, 1170, 1283, 1618, 1641, 1895
TaqII GACCGA 1 cut(s) 1152
TatI WGTACW 2 cut(s) 170, 260
TauI GCSGC 6 cut(s) 194, 549, 790, 2080, 2189, 2276
TfiI GAWTC 5 cut(s) 671, 1184, 1570, 1685, 1710
Tru1I TTAA 3 cut(s) 351, 722, 1455
Tru9I TTAA 3 cut(s) 351, 722, 1455
TscAI CASTG 8 cut(s) 229, 484, 1096, 1249, 1558, 1822, 1860, 1881
TseFI GTSAC 2 cut(s) 1991, 2085
Tsp45I GTSAC 2 cut(s) 1991, 2085
TspDTI ATGAA 7 cut(s) 245, 446, 874, 1380, 1689, 2001, 2172
TspGWI ACGGA 1 cut(s) 629
TspMI CCCGGG 1 cut(s) 1500
TspRI CASTG 8 cut(s) 229, 484, 1096, 1249, 1558, 1822, 1860, 1881
VpaK11BI GGWCC 3 cut(s) 44, 596, 1329
VspI ATTAAT 1 cut(s) 1455
XapI RAATTY 5 cut(s) 8, 946, 1016, 1085, 1971
XceI RCATGY 3 cut(s) 399, 518, 2096
XmaI CCCGGG 1 cut(s) 1500
XmiI GTMKAC 1 cut(s) 1940
XspI CTAG 4 cut(s) 866, 1295, 1403, 1734
ZraI GACGTC 1 cut(s) 2376
ZrmI AGTACT 2 cut(s) 172, 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.