MD13G1002600.v1.1
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010351
Physical Location & Seq
Forward (+)
153745 .. 162735
8991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1002600.v1.1.491

Sequence Viewer

Length: 2397 bp
ATGCTGAAATTCTTGAAGGGAGTGGTGGGCGGATCGGGGACTGGACCCAAAGATCTGCCCTACAACATCGGCGAACCTTACCTCTCTGCTTGGGGATCTTGGACTCACTTCCGAGGCACCTCCAAGGACGATGGTACTCCGGTGTCCATATTCTCTATATCTGGAAGTAGTGCTCAGGATGGCCATTTGGCCGCTGCTCGCAATGGCGTCAAGCGTCTCCGCACTGTCAGACACCCAAACATCTTATCATTTCTTCATAGTACTGAGGCTGAAACTGTGGATGCTTCTTCTGCCGTCAAGCAGATTATCTATATCGTTACCGAGCCTGTTATGCCACTCTCTGAAAAGATTAAGGAGCTCAATTTACAAGGTATCCAAAGGGATGAGTATTTCGCATGTGGACTGAACCAGATAGCTAAGGCTGTCAGTTTCTTGAATAATGATTGTAAACTTGTTCATGCCAACGTTTGCTTGGCCAGTGTTGTTGTAACTCAAACTTTGGACTGGAAGTTACATGCTTTTGATGTGCTATCTGAGTTTGATGGCGGCAATGAGTCTTCTGCCGGACAAATGCTGCAATTCGCCTGGCTTGTTGGACCACAATACAAACCCACGGAGTTGCTGAAGTCTGATTGGGCTGCAATCAGAAAGTCTCCACCATGGGCTATTGATTCTTGGGGCTTGGGCTGTCTTATATACGAGCTCTTTTCCGGTCTGAAGTTAAGCAAAACAGAGGAACTGCGCAACACAGCTTCTATTCCAAAGTCTCTGCTTCCAGATTATCAGCGGCTCTTGAGTTCCACGCCTTCTCGTAGGTTGAATACATCGAAGCTTATAGAAAATAGTGAATATTTCCAAAATAAGCTAGTGGATACAATACACTTCATGGAAATCCTAAATCTGAAAGATAGTGTTGAGAAGGATACCTTCTTCCGCAAGCTTCCAAATTTAGTGGAGCAGCTACCTCGCCAAATTGTGCTGAAAAAGTTGCTTCCTTTATTGGCTTCTGCCCTTGAATTTGGTTCAGCTGCTGCTCCTGCTTTGACGGCTTTCTTGAAAATGGGTTCATGGCTTTCAACCGAAGAATTCAGTGTGAAGGTGCTGCCAACAATTGTGAAACTGTTTGCTTCCAATGACCGAGCTATTCGAGTTGGACTTTTGCAACATATCGATCAGTTTGGAGAATCATTATCAGCACAAATTGTTGATGAGCAAGTTTACCCCCATGTTGCTACTGGGTTCACTGACACATCTGCTTTTCTCCGGGAACTGACTTTGAAGTCGATGCTTGTTCTAGCTCCTAAGCTTTCTCAACGCACCATTTCAGGGTCCTTGTTGAAGTATCTTTCAAAGTTACAGGTTGATGAAGAACCAGCAATTAGAACGAACACCACTATATTACTAGGGAACATTGCATCCCACCTGAACGATGGGACAAGGAAAAGAGTGCTGATTAATGCTTTTACTGCCCGTGCATTACGTGATACTTTCTCACCTGCCCGGGGAGCAGGTATTATGGCTTTGTGTGCTACCAGTTCTTATTATGATATCACTGAGATTGCAACTCGGATTCTCCCAAATGTTGTTATTCTTACCATTGATCCGGATAATGATGTTCGATCAAAGGCATTTCAAGCAGTTGATCAATTTTTACAAATAGTGAAGCAATCCTATGAAAAGACTGATTCAGGAGATACCGCCGGTGGTGGAATCTCATCACTACCAGGAAATGCTAGTTTACTGGGATGGGCAATGAGCTCCTTGACTCTCAAGGGTAAACCTTCTGAACAAGCTCCACTTGCTCCTGTGAATACCAGTGCATCTCTTACTAAAACAACTTCTAATGCAAGCTCAGTGGTGGATACTCCAAGCACAGTGCCGGCTCACGTAAGTTCGACAACAGATTTTGCCGACCACCATATAACTGAGTCCCCAACGTCTACTGATGGCTGGGGAGAACTTGAAAATGGAATTCACGAAGAGAATGAAAGTGACAAGGATGGGTGGGACGATGTTGAACCCCTGGAAGAGCCAAAACCGTATCCTGTTCTTGCGAACATTCAAGCAGCTCAAAAGCGGCCGGTGTCACAACATGTTTCGCAGCCTAAACAAGCAACGAGTTTGAGACCGAAAAATACAGCCAAGGCTGTCAAAAATGAAGATGATGATTTGTGGGGATCCATTGCCGCCCCTGCACCAAAAACAGTTTCAAAACCTTTGAATGTGAAAGCAAGTGCAGCAGTTGATGATGATGATGATCCATGGGCTGCGATTGCTGCTCCTCCACCCTCTACCAAGGCAAAGCCTCTATCAGTAGCCAAAGGCCGTGGAGCCAAAGCAGCTGCTCCAAAATTGGGCGCACAGAGGATAAACCGGACGTCTTCGTCCGGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

799

Amino Acids

86.46

Weight (kDa)

6.76

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 37 - 247 3.9e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1504
AasI GACNNNNNNGTC 2 cut(s) 1279, 2383
AatII GACGTC 1 cut(s) 2381
Acc16I TGCGCA 1 cut(s) 743
Acc36I ACCTGC 2 cut(s) 1499, 1504
AccB1I GGYRCC 1 cut(s) 116
AccI GTMKAC 1 cut(s) 1940
AccIII TCCGGA 1 cut(s) 1603
AciI CCGC 9 cut(s) 30, 192, 220, 546, 787, 934, 1698, 2077, 2187
AclI AACGTT 1 cut(s) 465
AclWI GGATC 6 cut(s) 40, 103, 1595, 2172, 2185, 2252
AcoI YGGCCR 4 cut(s) 181, 189, 474, 2078
AcsI RAATTY 5 cut(s) 8, 946, 1016, 1085, 1971
AcuI CTGAAG 2 cut(s) 644, 737
AcyI GRCGYC 2 cut(s) 207, 2378
AfaI GTAC 2 cut(s) 136, 262
AfiI CCNNNNNNNGG 3 cut(s) 1326, 1502, 2354
AflIII ACRYGT 1 cut(s) 2092
AjnI CCWGG 3 cut(s) 584, 1723, 2022
AjuI GAANNNNNNNTTGG 2 cut(s) 2191, 2223
AloI GAACNNNNNNTCC 2 cut(s) 1400, 1432
Alw21I GWGCWC 4 cut(s) 175, 360, 705, 1760
Alw26I GTCTC 4 cut(s) 221, 657, 771, 2119
AlwI GGATC 6 cut(s) 40, 103, 1595, 2172, 2185, 2252
AlwNI CAGNNNCTG 1 cut(s) 1031
Ama87I CYCGRG 1 cut(s) 1500
Aor13HI TCCGGA 1 cut(s) 1603
AoxI GGCC 5 cut(s) 181, 189, 474, 2078, 2323
ApoI RAATTY 5 cut(s) 8, 946, 1016, 1085, 1971
AseI ATTAAT 1 cut(s) 1455
AspLEI GCGC 2 cut(s) 744, 2360
AspS9I GGNCC 3 cut(s) 44, 596, 1329
AsuC2I CCSGG 4 cut(s) 1265, 1501, 1502, 2389
AsuHPI GGTGA 1 cut(s) 1485
AvaI CYCGRG 1 cut(s) 1500
AvaII GGWCC 3 cut(s) 44, 596, 1329
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BalI TGGCCA 2 cut(s) 183, 476
BamHI GGATCC 1 cut(s) 2177
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 3 cut(s) 360, 705, 1760
BbsI GAAGAC 2 cut(s) 549, 2373
Bbv12I GWGCWC 4 cut(s) 175, 360, 705, 1760
BccI CCATC 7 cut(s) 125, 173, 536, 1424, 1740, 1940, 1994
BceAI ACGGC 3 cut(s) 278, 1062, 2310
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 3 cut(s) 586, 1725, 2024
BciVI GTATCC 5 cut(s) 383, 865, 916, 1855, 2052
BclI TGATCA 1 cut(s) 1642
BcnI CCSGG 4 cut(s) 1265, 1501, 1502, 2389
BcoDI GTCTC 4 cut(s) 221, 657, 771, 2119
BfaI CTAG 4 cut(s) 866, 1295, 1403, 1734
BfuAI ACCTGC 2 cut(s) 1499, 1504
BfuI GTATCC 5 cut(s) 383, 865, 916, 1855, 2052
BglII AGATCT 1 cut(s) 52
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 7 cut(s) 586, 1265, 1501, 1502, 1725, 2024, 2389
Bme18I GGWCC 3 cut(s) 44, 596, 1329
BmeT110I CYCGRG 1 cut(s) 1500
BmgT120I GGNCC 3 cut(s) 44, 596, 1329
BmiI GGNNCC 5 cut(s) 46, 118, 1330, 2179, 2332
BmrFI CCNGG 7 cut(s) 586, 1265, 1501, 1502, 1725, 2024, 2389
BmrI ACTGGG 2 cut(s) 1245, 1751
BmsI GCATC 4 cut(s) 271, 1275, 1424, 1829
BmuI ACTGGG 2 cut(s) 1245, 1751
BpiI GAAGAC 2 cut(s) 549, 2373
Bpu10I CCTNAGC 3 cut(s) 174, 417, 1302
BpuEI CTTGAG 2 cut(s) 814, 1754
BpuMI CCSGG 4 cut(s) 1265, 1501, 1502, 2389
Bsa29I ATCGAT 1 cut(s) 1170
BsaAI YACGTR 2 cut(s) 1481, 1888
BsaBI GATNNNNATC 1 cut(s) 2256
BsaHI GRCGYC 2 cut(s) 207, 2378
BsaI GGTCTC 1 cut(s) 2119
BsaWI WCCGGW 4 cut(s) 139, 710, 1603, 2373
Bsc4I CCNNNNNNNGG 3 cut(s) 1326, 1502, 2354
Bse118I RCCGGY 3 cut(s) 1700, 1879, 2080
Bse1I ACTGG 7 cut(s) 46, 477, 509, 1240, 1533, 1746, 1815
Bse3DI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
Bse8I GATNNNNATC 1 cut(s) 2256
BseAI TCCGGA 1 cut(s) 1603
BseBI CCWGG 3 cut(s) 586, 1725, 2024
BseCI ATCGAT 1 cut(s) 1170
BseGI GGATG 6 cut(s) 184, 286, 388, 1415, 1751, 2005
BseJI GATNNNNATC 1 cut(s) 2256
BseLI CCNNNNNNNGG 3 cut(s) 1326, 1502, 2354
BseMI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
BseMII CTCAG 6 cut(s) 188, 255, 525, 1545, 1866, 1917
BseNI ACTGG 7 cut(s) 46, 477, 509, 1240, 1533, 1746, 1815
BseRI GAGGAG 1 cut(s) 2271
BseX3I CGGCCG 1 cut(s) 2078
BseYI CCCAGC 1 cut(s) 1950
BsgI GTGCAG 2 cut(s) 2178, 2256
Bsh1285I CGRYCG 1 cut(s) 2081
BshFI GGCC 5 cut(s) 183, 191, 476, 2080, 2325
BshNI GGYRCC 1 cut(s) 116
BshVI ATCGAT 1 cut(s) 1170
BsiEI CGRYCG 1 cut(s) 2081
BsiHKAI GWGCWC 4 cut(s) 175, 360, 705, 1760
BsiHKCI CYCGRG 1 cut(s) 1500
BslFI GGGAC 4 cut(s) 52, 1447, 1915, 2021
BslI CCNNNNNNNGG 3 cut(s) 1326, 1502, 2354
BsmAI GTCTC 4 cut(s) 221, 657, 771, 2119
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 4 cut(s) 52, 1447, 1915, 2021
BsnI GGCC 5 cut(s) 183, 191, 476, 2080, 2325
Bso31I GGTCTC 1 cut(s) 2119
BsoBI CYCGRG 1 cut(s) 1500
Bsp1286I GDGCHC 4 cut(s) 175, 360, 705, 1760
Bsp13I TCCGGA 1 cut(s) 1603
Bsp143I GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
Bsp19I CCATGG 2 cut(s) 659, 2261
BspACI CCGC 9 cut(s) 30, 192, 220, 546, 787, 934, 1698, 2077, 2187
BspANI GGCC 5 cut(s) 183, 191, 476, 2080, 2325
BspCNI CTCAG 6 cut(s) 187, 256, 526, 1546, 1865, 1918
BspDI ATCGAT 1 cut(s) 1170
BspEI TCCGGA 1 cut(s) 1603
BspLI GGNNCC 5 cut(s) 46, 118, 1330, 2179, 2332
BspMI ACCTGC 2 cut(s) 1499, 1504
BspPI GGATC 6 cut(s) 40, 103, 1595, 2172, 2185, 2252
BspQI GCTCTTC 1 cut(s) 2022
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 2119
BsrDI GCAATG 5 cut(s) 208, 556, 1410, 1758, 2181
BsrFI RCCGGY 3 cut(s) 1700, 1879, 2080
BsrI ACTGG 7 cut(s) 46, 477, 509, 1240, 1533, 1746, 1815
BssAI RCCGGY 3 cut(s) 1700, 1879, 2080
BssMI GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
BssNI GRCGYC 2 cut(s) 207, 2378
BssT1I CCWWGG 5 cut(s) 123, 659, 2142, 2261, 2295
Bst2UI CCWGG 3 cut(s) 586, 1725, 2024
Bst4CI ACNGT 6 cut(s) 226, 277, 1122, 1876, 2040, 2206
Bst6I CTCTTC 2 cut(s) 1974, 2022
BstACI GRCGYC 2 cut(s) 207, 2378
BstBAI YACGTR 2 cut(s) 1481, 1888
BstC8I GCNNGC 4 cut(s) 199, 938, 1849, 1881
BstDEI CTNAG 8 cut(s) 174, 264, 417, 534, 1302, 1554, 1852, 1926
BstDSI CCRYGG 4 cut(s) 612, 659, 2261, 2326
BstF5I GGATG 6 cut(s) 184, 286, 388, 1415, 1751, 2005
BstHHI GCGC 2 cut(s) 744, 2360
BstKTI GATC 9 cut(s) 35, 55, 98, 1174, 1603, 1622, 1645, 2180, 2260
BstMAI GTCTC 4 cut(s) 221, 657, 771, 2119
BstMBI GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
BstMCI CGRYCG 1 cut(s) 2081
BstNI CCWGG 3 cut(s) 586, 1725, 2024
BstNSI RCATGY 3 cut(s) 399, 518, 2096
BstSCI CCNGG 7 cut(s) 584, 1263, 1499, 1500, 1723, 2022, 2387
BstV2I GAAGAC 2 cut(s) 549, 2373
BstX2I RGATCY 3 cut(s) 52, 95, 2177
BstYI RGATCY 3 cut(s) 52, 95, 2177
BstZI CGGCCG 1 cut(s) 2078
Bsu15I ATCGAT 1 cut(s) 1170
BsuI GTATCC 5 cut(s) 383, 865, 916, 1855, 2052
BsuRI GGCC 5 cut(s) 183, 191, 476, 2080, 2325
BsuTUI ATCGAT 1 cut(s) 1170
BtgI CCRYGG 4 cut(s) 612, 659, 2261, 2326
BtsCI GGATG 6 cut(s) 184, 286, 388, 1415, 1751, 2005
BtsIMutI CAGTG 8 cut(s) 222, 484, 1096, 1242, 1551, 1822, 1860, 1881
BveI ACCTGC 2 cut(s) 1499, 1504
Cac8I GCNNGC 4 cut(s) 199, 938, 1849, 1881
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 2 cut(s) 744, 2360
Cfr10I RCCGGY 3 cut(s) 1700, 1879, 2080
Cfr13I GGNCC 3 cut(s) 44, 596, 1329
Cfr9I CCCGGG 1 cut(s) 1500
ClaI ATCGAT 1 cut(s) 1170
CseI GACGC 2 cut(s) 196, 203
Csp6I GTAC 2 cut(s) 135, 261
CspCI CAANNNNNGTGG 6 cut(s) 933, 968, 1836, 1871, 2308, 2343
CviAII CATG 9 cut(s) 396, 458, 515, 660, 886, 1068, 1226, 2093, 2262
CviQI GTAC 2 cut(s) 135, 261
DdeI CTNAG 8 cut(s) 174, 264, 417, 534, 1302, 1554, 1852, 1926
DpnI GATC 9 cut(s) 34, 54, 97, 1173, 1602, 1621, 1644, 2179, 2259
DpnII GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
DrdI GACNNNNNNGTC 2 cut(s) 1279, 2383
DseDI GACNNNNNNGTC 2 cut(s) 1279, 2383
EaeI YGGCCR 4 cut(s) 181, 189, 474, 2078
EagI CGGCCG 1 cut(s) 2078
Eam1104I CTCTTC 2 cut(s) 1974, 2022
EarI CTCTTC 2 cut(s) 1974, 2022
EciI GGCGGA 1 cut(s) 45
Ecl136II GAGCTC 3 cut(s) 358, 703, 1758
EclXI CGGCCG 1 cut(s) 2078
Eco130I CCWWGG 5 cut(s) 123, 659, 2142, 2261, 2295
Eco24I GRGCYC 3 cut(s) 360, 705, 1760
Eco31I GGTCTC 1 cut(s) 2119
Eco32I GATATC 1 cut(s) 1549
Eco47I GGWCC 3 cut(s) 44, 596, 1329
Eco52I CGGCCG 1 cut(s) 2078
Eco53kI GAGCTC 3 cut(s) 358, 703, 1758
Eco57I CTGAAG 2 cut(s) 644, 737
Eco88I CYCGRG 1 cut(s) 1500
EcoICRI GAGCTC 3 cut(s) 358, 703, 1758
EcoO109I RGGNCCY 1 cut(s) 1329
EcoRI GAATTC 2 cut(s) 1085, 1971
EcoRII CCWGG 3 cut(s) 584, 1723, 2022
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 5 cut(s) 123, 659, 2142, 2261, 2295
EcoT38I GRGCYC 3 cut(s) 360, 705, 1760
ErhI CCWWGG 5 cut(s) 123, 659, 2142, 2261, 2295
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 9 cut(s) 399, 461, 518, 663, 889, 1071, 1229, 2096, 2265
FalI AAGNNNNNCTT 4 cut(s) 911, 943, 1782, 1814
FaqI GGGAC 4 cut(s) 52, 1447, 1915, 2021
FatI CATG 9 cut(s) 395, 457, 514, 659, 885, 1067, 1225, 2092, 2261
FbaI TGATCA 1 cut(s) 1642
FblI GTMKAC 1 cut(s) 1940
FokI GGATG 6 cut(s) 191, 293, 395, 1402, 1758, 2012
FriOI GRGCYC 3 cut(s) 360, 705, 1760
FspBI CTAG 4 cut(s) 866, 1295, 1403, 1734
FspI TGCGCA 1 cut(s) 743
GlaI GCGC 2 cut(s) 743, 2359
GsaI CCCAGC 1 cut(s) 1954
HaeIII GGCC 5 cut(s) 183, 191, 476, 2080, 2325
HgaI GACGC 2 cut(s) 196, 203
HhaI GCGC 2 cut(s) 744, 2360
Hin1I GRCGYC 2 cut(s) 207, 2378
Hin1II CATG 9 cut(s) 399, 461, 518, 663, 889, 1071, 1229, 2096, 2265
Hin6I GCGC 2 cut(s) 742, 2358
HinP1I GCGC 2 cut(s) 742, 2358
HindIII AAGCTT 3 cut(s) 830, 938, 1304
HinfI GANTC 9 cut(s) 103, 554, 671, 1184, 1570, 1685, 1710, 1765, 1928
HphI GGTGA 1 cut(s) 1485
Hpy166II GTNNAC 7 cut(s) 401, 449, 1219, 1242, 1739, 1778, 1941
Hpy8I GTNNAC 7 cut(s) 401, 449, 1219, 1242, 1739, 1778, 1941
HpyAV CCTTC 6 cut(s) 10, 816, 913, 937, 1090, 1791
HpyCH4III ACNGT 6 cut(s) 226, 277, 1122, 1876, 2040, 2206
HpyCH4IV ACGT 5 cut(s) 465, 1480, 1887, 1937, 2378
HpyF3I CTNAG 8 cut(s) 174, 264, 417, 534, 1302, 1554, 1852, 1926
HpySE526I ACGT 5 cut(s) 465, 1480, 1887, 1937, 2378
Hsp92I GRCGYC 2 cut(s) 207, 2378
Hsp92II CATG 9 cut(s) 399, 461, 518, 663, 889, 1071, 1229, 2096, 2265
HspAI GCGC 2 cut(s) 742, 2358
Kpn2I TCCGGA 1 cut(s) 1603
KroI GCCGGC 1 cut(s) 1879
KroNI GCCGGC 1 cut(s) 1881
Ksp22I TGATCA 1 cut(s) 1642
Kzo9I GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
LguI GCTCTTC 1 cut(s) 2022
LweI GCATC 4 cut(s) 271, 1275, 1424, 1829
MaeI CTAG 4 cut(s) 866, 1295, 1403, 1734
MaeII ACGT 5 cut(s) 465, 1480, 1887, 1937, 2378
MaeIII GTNAC 6 cut(s) 316, 487, 510, 1353, 1991, 2085
MalI GATC 9 cut(s) 34, 54, 97, 1173, 1602, 1621, 1644, 2179, 2259
MboI GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 3 cut(s) 52, 95, 2177
MhlI GDGCHC 4 cut(s) 175, 360, 705, 1760
MlsI TGGCCA 2 cut(s) 183, 476
MluNI TGGCCA 2 cut(s) 183, 476
MlyI GAGTC 4 cut(s) 97, 563, 1759, 1937
MmeI TCCRAC 2 cut(s) 574, 1132
Mox20I TGGCCA 2 cut(s) 183, 476
MroI TCCGGA 1 cut(s) 1603
MroNI GCCGGC 1 cut(s) 1879
MscI TGGCCA 2 cut(s) 183, 476
MseI TTAA 3 cut(s) 351, 722, 1455
Msp20I TGGCCA 2 cut(s) 183, 476
MspA1I CMGCKG 4 cut(s) 194, 787, 1028, 2342
MspR9I CCNGG 7 cut(s) 586, 1265, 1501, 1502, 1725, 2024, 2389
MunI CAATTG 1 cut(s) 1110
MvaI CCWGG 3 cut(s) 586, 1725, 2024
NaeI GCCGGC 1 cut(s) 1881
NciI CCSGG 4 cut(s) 1265, 1501, 1502, 2389
NcoI CCATGG 2 cut(s) 659, 2261
NdeII GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
NgoMIV GCCGGC 1 cut(s) 1879
NlaIII CATG 9 cut(s) 399, 461, 518, 663, 889, 1071, 1229, 2096, 2265
NlaIV GGNNCC 5 cut(s) 46, 118, 1330, 2179, 2332
NmuCI GTSAC 2 cut(s) 1991, 2085
NsbI TGCGCA 1 cut(s) 743
NspI RCATGY 3 cut(s) 399, 518, 2096
PaqCI CACCTGC 1 cut(s) 1504
PciI ACATGT 1 cut(s) 2092
PciSI GCTCTTC 1 cut(s) 2022
PdiI GCCGGC 1 cut(s) 1881
PfeI GAWTC 5 cut(s) 671, 1184, 1570, 1685, 1710
PfoI TCCNGGA 1 cut(s) 1263
PleI GAGTC 4 cut(s) 97, 562, 1759, 1936
PpsI GAGTC 4 cut(s) 97, 562, 1759, 1936
Ppu21I YACGTR 2 cut(s) 1481, 1888
PpuMI RGGWCCY 1 cut(s) 1329
PscI ACATGT 1 cut(s) 2092
PshBI ATTAAT 1 cut(s) 1455
Psp124BI GAGCTC 3 cut(s) 360, 705, 1760
Psp1406I AACGTT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 1329
Psp6I CCWGG 3 cut(s) 584, 1723, 2022
PspFI CCCAGC 1 cut(s) 1950
PspGI CCWGG 3 cut(s) 584, 1723, 2022
PspN4I GGNNCC 5 cut(s) 46, 118, 1330, 2179, 2332
PspPI GGNCC 3 cut(s) 44, 596, 1329
PspPPI RGGWCCY 1 cut(s) 1329
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 3 cut(s) 52, 95, 2177
PvuII CAGCTG 2 cut(s) 1028, 2342
RsaI GTAC 2 cut(s) 136, 262
RsaNI GTAC 2 cut(s) 135, 261
SacI GAGCTC 3 cut(s) 360, 705, 1760
SapI GCTCTTC 1 cut(s) 2022
SaqAI TTAA 3 cut(s) 351, 722, 1455
Sau3AI GATC 9 cut(s) 32, 52, 95, 1171, 1600, 1619, 1642, 2177, 2257
Sau96I GGNCC 3 cut(s) 44, 596, 1329
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 4 cut(s) 97, 563, 1759, 1937
ScrFI CCNGG 7 cut(s) 586, 1265, 1501, 1502, 1725, 2024, 2389
SduI GDGCHC 4 cut(s) 175, 360, 705, 1760
SfaNI GCATC 4 cut(s) 271, 1275, 1424, 1829
SgrAI CRCCGGYG 1 cut(s) 1700
SinI GGWCC 3 cut(s) 44, 596, 1329
SmaI CCCGGG 1 cut(s) 1502
SmlI CTYRAG 2 cut(s) 793, 1769
SmoI CTYRAG 2 cut(s) 793, 1769
SsiI CCGC 9 cut(s) 30, 192, 220, 546, 787, 934, 1698, 2077, 2187
SspI AATATT 1 cut(s) 851
SspMI CTAG 4 cut(s) 866, 1295, 1403, 1734
SstI GAGCTC 3 cut(s) 360, 705, 1760
StyD4I CCNGG 7 cut(s) 584, 1263, 1499, 1500, 1723, 2022, 2387
StyI CCWWGG 5 cut(s) 123, 659, 2142, 2261, 2295
TaaI ACNGT 6 cut(s) 226, 277, 1122, 1876, 2040, 2206
TaiI ACGT 5 cut(s) 468, 1483, 1890, 1940, 2381
TaqI TCGA 6 cut(s) 827, 1147, 1170, 1283, 1618, 1895
TaqII GACCGA 2 cut(s) 1152, 2143
TatI WGTACW 1 cut(s) 260
TauI GCSGC 5 cut(s) 194, 549, 790, 2080, 2189
TfiI GAWTC 5 cut(s) 671, 1184, 1570, 1685, 1710
Tru1I TTAA 3 cut(s) 351, 722, 1455
Tru9I TTAA 3 cut(s) 351, 722, 1455
TscAI CASTG 8 cut(s) 229, 484, 1096, 1249, 1558, 1822, 1860, 1881
TseFI GTSAC 2 cut(s) 1991, 2085
Tsp45I GTSAC 2 cut(s) 1991, 2085
TspDTI ATGAA 8 cut(s) 245, 446, 874, 1056, 1380, 1689, 2001, 2172
TspGWI ACGGA 1 cut(s) 629
TspMI CCCGGG 1 cut(s) 1500
TspRI CASTG 8 cut(s) 229, 484, 1096, 1249, 1558, 1822, 1860, 1881
VpaK11BI GGWCC 3 cut(s) 44, 596, 1329
VspI ATTAAT 1 cut(s) 1455
XapI RAATTY 5 cut(s) 8, 946, 1016, 1085, 1971
XceI RCATGY 3 cut(s) 399, 518, 2096
XcmI CCANNNNNNNNNTGG 3 cut(s) 469, 1232, 1427
XmaI CCCGGG 1 cut(s) 1500
XmiI GTMKAC 1 cut(s) 1940
XspI CTAG 4 cut(s) 866, 1295, 1403, 1734
ZraI GACGTC 1 cut(s) 2379
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.