Rorug04G0376900
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010351
Physical Location & Seq
Reverse (-)
54762893 .. 54768548
5656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0376900.1

Sequence Viewer

Length: 3510 bp
ATGAGCGCCATCGATGATGAGATAGAGGCCTCGCAGCGGGTGGTGGATTTGATGCCAGCTTTCCCTTGGCTAGTTGGGCTTTTTGCTTCAGAGGAGAAGATGGTGGCTACCCAACATCGACCTCTGGGTCAGATTGTGCCACTATGCTCTTTCTTTGGGTTTTTTGATCCAGGTGGAGGTGGTGATGCTGTCGGCGGCGGCGGCGATACTGATGGAGGTGCTCCTAGCCAATTTGTTCCAATGGTGGTTAAGGTTGTTTTGGGCCCTCGGGTTGTCTTAAGCCCTAGTGGTGGTGATGTTGTCCATGAAGGGATTGCGGGCTGTGGCAATGGCAACGCAAATGGTGTTGGTAAAGCCGGTGGTGTCGGCACTATTGCTAATGGCGACGGCGCTAAATTTGGGGTTCAGAGCAGGAAGGCCATAGTGCAGAACTACTCCGATTGGATGTCGCTCAAGGAATATGGAAGTTCAATTGAGAGTCTTGCAGAGTTCATATTGGCATCCATTTCTGAGATCACAGTATTGGTAGTAAGCGTAGTGGTGGAACAAGAAAGTTTTATTGAAACTGCATGCTACCTTTATCGGGCTTCTATGGCCATAATGGAGTTGATGACCACCGACTTCACATGCCAAGAAAATGCCATGGAAATTTTTCAGTCTATTTCAACAAGTGTTAGTTTGGCCAAACTTCTTGTGGAGAACTGCCAAAAAGGCATTCAACCATTTTCGGATCCTGAACTCATTACCCTTACAGCGCAGCTGGAGGAGGTCATCAAGCACGTAGGAGAATGCTTGAGCTTCATACAGCCATCAACATTTGGAGACCAACATTACGCAGAAAGTGCAGTTCGGTCCCTTTCAAATGAGATGCAGACTGCTCACTTTGAAGCTCAAGCATCCAAGACCAATGAGATAGATAAAAAGATGTTGTCCTTCAGAAAACAACCAAAGGTAGAACCGACATCAACACCAACAGAAATGGATCTCTACCCTATAAGTTTCGAGATCTCCACAGAAAATCCTCAGTTCTTAAATACGCCGCAGTTGATTGAAATGCTTAAGAGCACAAGTTGGGTCAGCGAAAGAAATGAGGAGAATATCAGTGGACCATTAACAACATTGCCTCCAGTTGGTCAGTACATGGAGCCTATGTATGAGACTTTCTTCTGTCCACTGACAAAGAAGATCATGGAGGATCCAGTAACCATAAGAAGTGGAGTGACCTTTGAGAGGAAGGCAATTGTTGAGTGGTTTGAGAAGCTCACTGGCTCAGAACAAATATTGTGCCCAACCACTGGCCAGAAGTTGATGAGTAAAGCTTTCAATGAGAACATAGCTCTGAAAACCACAATAGAGCAGTGGAAAGAAAGGAATGAAGCAGCTAGGATTAAGGTTGCCCGCTCAGCATTGTCCTTGGCTAGTTCAGATGCAATGGTACTTGAAGCAGTAAAAGAGGTGCAAAGTATCTACCAAAGGAAGCCATACAACAACACTCAAGTTTGCAGTGTTGGAATATTGCCATTGCTTGTTCAGTGCCTAGAGTACAAAGACAAAGATGTTAGAAGCACAGTCCTAGAGCTTTTACTACAACTGATGGAGGATGACAATGAAAGCAAGGAAATGATTGCTGAAATCATCAACATTCCTACTATAATTGAAATGCTTTCTAGTAGTCACCAGTCCATAAGGCATGCATCATTGCTATTCTTGCTTGAGCTTTCAAGATCAGAAGCATTATGTGAGAAAATTGGATCAGTTACTGGAGCAATTCTTATGTTGATCACGATTAAATATAGACGATCATATGATGCATTCGCTTCGGAAAAAGCAGACGAGACCTTAAGGAACTTAGAGCTTTCGCCAAACAACATCAAACTCATGGCAGAAAATGGACTAGTGGAACCCCTCCTAAACAATCTTACTGAAGGTTGTGAAGAGATGAAAATAGACATGGCAAGTTACCTTGGGGAAATTGTTCTTGGACTTGACAGCAAAACCTATGTTGCTGAAGGGGCCTCTCCTACTCTCATCAAAATGGTACATAGAGGAAATACACTGGCCAGAGGCGCGGCATTCAAAACTCTAGTGCTACTCTCCTCGTACCAGCCAAATGCCAACATACTTATAGAAGCTGGAATTGTACAAGTCATGGTTGAAGAGATGTTCATCCGGAAAGTTCACAACGAACCAATGAACTCAAAAAGTGAAGCAGCTGCAATCCTTGCAAATATATTCGAGTCTGATCTTGAGCTTGAGAACCTCCAGGTAAACTCTCACGGTCACACCATGACTTCAGACTATGTTGTGTACAACATCCTTTACATGCTCAACAACTCAACCCCAGATGAACTAAACAACAATCTTATCAGGATTCTTTTAGTTCTTGCTAAGATCCCCAAACCAGAGGCCACCATTGTGTCATTGGTAAAAGAGACTGAAGCAAGCTACACCCTCATAGAACTCATCAACAACCCCCATGAAGAACTTTCCATTGCAGCAATCAAGCTACTAACTGTTCTTTCACCCCATATGGGCCACCTTCTAGCCGAGAGACTCTGCAAAACCAGAGGTCAACCAGAGAACCTTCTTATCGAGAGCTCAACTGAGACGACCCTCATTACTGAGAAGCAGGCAGTTTCAGCAAAATTTCTAGCAAAACTTCCCCACCAAAATCTGACACTTAATCTAGCTCTTCTATATAAGAATATTGTGCCAACAATCATACAAGCAATCAATCAAATTCAAAGAAGAGGAACAAGATCAAGCAGGTTTGAAAGCACTTACTTTGAAGGTCTAGTGGGCATTCTAGTCAGATTCACTACAACACTATATGAACCTCAAATGTTGTTTCTAGCAAGAAACCAGCATTTCACAGCAGTTTTCACAGAACTGCTCGTGAAAACATCAAGTGATGAAGTTCAGAGGTTATCAGCAATTGGGTTGGAGAATCTCTCTTCGGAGTCAATAAGCTTATCGAAACCACCGCAGATTAAGAGAAAAAAGTTCATGAAGTTATTCTACCTGCCTAAATATCTATCTTTTGGCTATTCAAGGAGGAGGAGAATACCATTGTGCCCGATTCACCGAGGAGCCTGTTCCTCTCAAAACACCTTCTGCATAGTTGATGCAAAGGCAGTGGAGAAGCTACTAGACTGTTTGGAAAACGAGAATCCAGAGGTTGTTGAAGCTGCATTGTCAGCTATAAGCACTTTGTTGGATGACAAGGTGGATGTGGACAAGTCTGTGAGTATGTTGAGTGAGGTTAACGCTGTGCAACATGTTTTGAATGCAGTGAAAGAGCATAGGCAAGAAGGATTGCGGCAGAAGTCATTTTGGGTGCTTGAAAAGTTCTTGAATAAAGGCAGTGATCAGTCTGCTTCAGATATATCAAATGACAGGGTGTTGCCTGCTATATTGGTCAGTGCTTTCCATCATGGAGCTGGAAATACTAGGCAGATGGCTGAGAGGATTTTGAGGCATTTGAATAAGATGCCCAACCTCTATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

1169

Amino Acids

129.57

Weight (kDa)

5.35

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 386 - 459 3.6e-13 U-box domain
ARM_LIN_C PF23628 494 - 573 8.3e-09 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Arm PF00514 1033 - 1071 8.6e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 126
Acc36I ACCTGC 2 cut(s) 2757, 3030
AccB7I CCANNNNNTGG 1 cut(s) 1295
AccBSI CCGCTC 1 cut(s) 1401
AccII CGCG 1 cut(s) 2069
AccIII TCCGGA 1 cut(s) 2169
AclWI GGATC 8 cut(s) 161, 725, 738, 990, 1190, 1203, 1759, 2386
AcoI YGGCCR 4 cut(s) 594, 681, 1297, 2058
AcsI RAATTY 4 cut(s) 395, 648, 2645, 2739
AcuI CTGAAG 7 cut(s) 72, 919, 1944, 2028, 2277, 2457, 3363
AfaI GTAC 7 cut(s) 1139, 1437, 1544, 2040, 2102, 2142, 2309
AfiI CCNNNNNNNGG 8 cut(s) 36, 176, 290, 583, 1130, 1230, 1295, 2531
AflII CTTAAG 3 cut(s) 277, 1058, 1840
AflIII ACRYGT 1 cut(s) 3277
AhlI ACTAGT 1 cut(s) 1894
AjnI CCWGG 2 cut(s) 169, 2262
AjuI GAANNNNNNNTTGG 4 cut(s) 699, 731, 1962, 1994
AleI CACNNNNGTG 1 cut(s) 2414
Alw21I GWGCWC 3 cut(s) 223, 1067, 2600
Alw26I GTCTC 6 cut(s) 816, 1151, 1829, 2426, 2545, 2600
AlwI GGATC 8 cut(s) 161, 725, 738, 990, 1190, 1203, 1759, 2386
AlwNI CAGNNNCTG 1 cut(s) 1760
Ama87I CYCGRG 1 cut(s) 267
Aor13HI TCCGGA 1 cut(s) 2169
ApaI GGGCCC 1 cut(s) 266
ApeKI GCWGC 7 cut(s) 34, 757, 1379, 2210, 2213, 2495, 3188
ApoI RAATTY 4 cut(s) 395, 648, 2645, 2739
Asp700I GAANNNNTTC 3 cut(s) 651, 1974, 3014
AspLEI GCGC 4 cut(s) 8, 392, 757, 2069
AspS9I GGNCC 6 cut(s) 262, 263, 852, 1106, 2013, 2533
AsuHPI GGTGA 5 cut(s) 194, 305, 1667, 2514, 3074
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 2 cut(s) 852, 1106
BaeGI GKGCMC 3 cut(s) 266, 1289, 3077
BalI TGGCCA 4 cut(s) 596, 683, 1299, 2060
BamHI GGATCC 2 cut(s) 730, 1195
BanII GRGCYC 2 cut(s) 266, 2600
BarI GAAGNNNNNNTAC 2 cut(s) 3002, 3034
BauI CACGAG 1 cut(s) 2894
Bbv12I GWGCWC 3 cut(s) 223, 1067, 2600
BbvI GCAGC 7 cut(s) 46, 769, 1391, 2200, 2222, 2507, 3175
BccI CCATC 7 cut(s) 17, 94, 206, 817, 1588, 3438, 3451
BceAI ACGGC 1 cut(s) 403
BcgI CGANNNNNNTGC 2 cut(s) 1800, 1834
BciT130I CCWGG 2 cut(s) 171, 2264
BclI TGATCA 2 cut(s) 1779, 3367
BcoDI GTCTC 6 cut(s) 816, 1151, 1829, 2426, 2545, 2600
BcuI ACTAGT 1 cut(s) 1894
BfoI RGCGCY 2 cut(s) 9, 393
BfrI CTTAAG 3 cut(s) 277, 1058, 1840
BfuAI ACCTGC 2 cut(s) 2757, 3030
BglI GCCNNNNNGGC 1 cut(s) 711
BglII AGATCT 1 cut(s) 1005
BlpI GCTNAGC 1 cut(s) 1402
Bme1390I CCNGG 2 cut(s) 171, 2264
Bme18I GGWCC 2 cut(s) 852, 1106
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 6 cut(s) 262, 263, 852, 1106, 2013, 2533
BmiI GGNNCC 8 cut(s) 264, 732, 854, 1146, 1197, 1902, 2014, 3091
BmrFI CCNGG 2 cut(s) 171, 2264
BplI GAGNNNNNCTC 2 cut(s) 2936, 2968
BpmI CTGGAG 4 cut(s) 782, 1110, 1782, 2246
Bpu1102I GCTNAGC 1 cut(s) 1402
BpuEI CTTGAG 7 cut(s) 437, 814, 876, 1479, 1733, 2267, 2273
Bsa29I ATCGAT 1 cut(s) 12
BsaAI YACGTR 1 cut(s) 781
BsaBI GATNNNNATC 1 cut(s) 2165
BsaI GGTCTC 2 cut(s) 816, 1829
BsaJI CCNNGG 6 cut(s) 65, 266, 642, 1413, 1963, 3085
BsaWI WCCGGW 1 cut(s) 2169
BsaXI ACNNNNNCTCC 4 cut(s) 1108, 1136, 1138, 1166
Bsc4I CCNNNNNNNGG 8 cut(s) 36, 176, 290, 583, 1130, 1230, 1295, 2531
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 7 cut(s) 1127, 1199, 1270, 1300, 1678, 1765, 2061
Bse3DI GCAATG 6 cut(s) 334, 1118, 1437, 1520, 1697, 2490
Bse8I GATNNNNATC 1 cut(s) 2165
BseAI TCCGGA 1 cut(s) 2169
BseBI CCWGG 2 cut(s) 171, 2264
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 6 cut(s) 65, 266, 642, 1413, 1963, 3085
BseGI GGATG 8 cut(s) 450, 500, 896, 1606, 2166, 2313, 3223, 3235
BseJI GATNNNNATC 1 cut(s) 2165
BseLI CCNNNNNNNGG 8 cut(s) 36, 176, 290, 583, 1130, 1230, 1295, 2531
BseMI GCAATG 6 cut(s) 334, 1118, 1437, 1520, 1697, 2490
BseMII CTCAG 7 cut(s) 501, 1037, 1284, 1416, 2595, 2613, 3453
BseNI ACTGG 7 cut(s) 1127, 1199, 1270, 1300, 1678, 1765, 2061
BseRI GAGGAG 7 cut(s) 107, 779, 1106, 2086, 3070, 3073, 3102
BseSI GKGCMC 3 cut(s) 266, 1289, 3077
BseXI GCAGC 7 cut(s) 46, 769, 1391, 2200, 2222, 2507, 3175
BsgI GTGCAG 2 cut(s) 446, 864
Bsh1236I CGCG 1 cut(s) 2069
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 223, 1067, 2600
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 2 cut(s) 357, 2170
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 8 cut(s) 36, 176, 290, 583, 1130, 1230, 1295, 2531
BsmAI GTCTC 6 cut(s) 816, 1151, 1829, 2426, 2545, 2600
BsmBI CGTCTC 1 cut(s) 2600
BsmFI GGGAC 1 cut(s) 838
BsmI GAATGC 6 cut(s) 714, 794, 1811, 2072, 2802, 3292
Bso31I GGTCTC 2 cut(s) 816, 1829
BsoBI CYCGRG 1 cut(s) 267
Bsp120I GGGCCC 1 cut(s) 262
Bsp1286I GDGCHC 6 cut(s) 223, 266, 1067, 1289, 2600, 3077
Bsp13I TCCGGA 1 cut(s) 2169
Bsp1407I TGTACA 2 cut(s) 2140, 2307
Bsp1720I GCTNAGC 1 cut(s) 1402
Bsp19I CCATGG 1 cut(s) 642
BspCNI CTCAG 7 cut(s) 502, 1036, 1283, 1415, 2596, 2614, 3454
BspDI ATCGAT 1 cut(s) 12
BspEI TCCGGA 1 cut(s) 2169
BspFNI CGCG 1 cut(s) 2069
BspHI TCATGA 1 cut(s) 3006
BspLI GGNNCC 8 cut(s) 264, 732, 854, 1146, 1197, 1902, 2014, 3091
BspMI ACCTGC 2 cut(s) 2757, 3030
BspPI GGATC 8 cut(s) 161, 725, 738, 990, 1190, 1203, 1759, 2386
BspQI GCTCTTC 1 cut(s) 2697
BspTI CTTAAG 3 cut(s) 277, 1058, 1840
BspTNI GGTCTC 2 cut(s) 816, 1829
BsrBI CCGCTC 1 cut(s) 1401
BsrDI GCAATG 6 cut(s) 334, 1118, 1437, 1520, 1697, 2490
BsrFI RCCGGY 1 cut(s) 356
BsrGI TGTACA 2 cut(s) 2140, 2307
BsrI ACTGG 7 cut(s) 1127, 1199, 1270, 1300, 1678, 1765, 2061
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 6 cut(s) 65, 266, 642, 1413, 1963, 3085
BssSI CACGAG 1 cut(s) 2894
BssT1I CCWWGG 4 cut(s) 65, 642, 1413, 1963
Bst2BI CACGAG 1 cut(s) 2894
Bst2UI CCWGG 2 cut(s) 171, 2264
Bst4CI ACNGT 5 cut(s) 520, 1570, 2279, 2515, 3155
Bst6I CTCTTC 5 cut(s) 1929, 2151, 2697, 2743, 2959
BstAFI CTTAAG 3 cut(s) 277, 1058, 1840
BstAPI GCANNNNNTGC 2 cut(s) 842, 2222
BstAUI TGTACA 2 cut(s) 2140, 2307
BstBAI YACGTR 1 cut(s) 781
BstC8I GCNNGC 8 cut(s) 57, 319, 571, 1399, 1692, 2443, 2631, 3408
BstDEI CTNAG 9 cut(s) 510, 1023, 1270, 1402, 1849, 2388, 2604, 2622, 3462
BstDSI CCRYGG 1 cut(s) 642
BstENI CCTNNNNNAGG 1 cut(s) 1228
BstF5I GGATG 8 cut(s) 450, 500, 896, 1606, 2166, 2313, 3223, 3235
BstFNI CGCG 1 cut(s) 2069
BstH2I RGCGCY 2 cut(s) 9, 393
BstHHI GCGC 4 cut(s) 8, 392, 757, 2069
BstMAI GTCTC 6 cut(s) 816, 1151, 1829, 2426, 2545, 2600
BstNI CCWGG 2 cut(s) 171, 2264
BstNSI RCATGY 5 cut(s) 573, 630, 1694, 2326, 3281
BstSCI CCNGG 2 cut(s) 169, 2262
BstSLI GKGCMC 3 cut(s) 266, 1289, 3077
BstUI CGCG 1 cut(s) 2069
BstV1I GCAGC 7 cut(s) 46, 769, 1391, 2200, 2222, 2507, 3175
BstX2I RGATCY 5 cut(s) 730, 982, 1005, 1195, 2391
BstYI RGATCY 5 cut(s) 730, 982, 1005, 1195, 2391
Bsu15I ATCGAT 1 cut(s) 12
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 8 cut(s) 450, 500, 896, 1606, 2166, 2313, 3223, 3235
BtsI GCAGTG 5 cut(s) 1364, 1510, 3141, 3297, 3370
BveI ACCTGC 2 cut(s) 2757, 3030
Cac8I GCNNGC 8 cut(s) 57, 319, 571, 1399, 1692, 2443, 2631, 3408
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 1 cut(s) 3006
CfoI GCGC 4 cut(s) 8, 392, 757, 2069
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 6 cut(s) 262, 263, 852, 1106, 2013, 2533
ClaI ATCGAT 1 cut(s) 12
Csp6I GTAC 7 cut(s) 1138, 1436, 1543, 2039, 2101, 2141, 2308
CspCI CAANNNNNGTGG 2 cut(s) 3117, 3152
CviQI GTAC 7 cut(s) 1138, 1436, 1543, 2039, 2101, 2141, 2308
DdeI CTNAG 9 cut(s) 510, 1023, 1270, 1402, 1849, 2388, 2604, 2622, 3462
DrdI GACNNNNNNGTC 1 cut(s) 126
DseDI GACNNNNNNGTC 1 cut(s) 126
EaeI YGGCCR 4 cut(s) 594, 681, 1297, 2058
Eam1104I CTCTTC 5 cut(s) 1929, 2151, 2697, 2743, 2959
EarI CTCTTC 5 cut(s) 1929, 2151, 2697, 2743, 2959
Ecl136II GAGCTC 1 cut(s) 2598
Eco130I CCWWGG 4 cut(s) 65, 642, 1413, 1963
Eco147I AGGCCT 1 cut(s) 29
Eco24I GRGCYC 2 cut(s) 266, 2600
Eco31I GGTCTC 2 cut(s) 816, 1829
Eco47I GGWCC 2 cut(s) 852, 1106
Eco53kI GAGCTC 1 cut(s) 2598
Eco57I CTGAAG 7 cut(s) 72, 919, 1944, 2028, 2277, 2457, 3363
Eco88I CYCGRG 1 cut(s) 267
EcoICRI GAGCTC 1 cut(s) 2598
EcoNI CCTNNNNNAGG 1 cut(s) 1228
EcoO109I RGGNCCY 2 cut(s) 263, 2013
EcoRII CCWGG 2 cut(s) 169, 2262
EcoT14I CCWWGG 4 cut(s) 65, 642, 1413, 1963
EcoT22I ATGCAT 2 cut(s) 1696, 1813
EcoT38I GRGCYC 2 cut(s) 266, 2600
ErhI CCWWGG 4 cut(s) 65, 642, 1413, 1963
Esp3I CGTCTC 1 cut(s) 2600
FaqI GGGAC 1 cut(s) 838
FauI CCCGC 3 cut(s) 30, 310, 1406
FauNDI CATATG 2 cut(s) 1804, 2529
FbaI TGATCA 2 cut(s) 1779, 3367
FokI GGATG 8 cut(s) 457, 487, 883, 1613, 2153, 2300, 3230, 3242
FriOI GRGCYC 2 cut(s) 266, 2600
GlaI GCGC 4 cut(s) 7, 391, 756, 2068
GsuI CTGGAG 4 cut(s) 782, 1110, 1782, 2246
HaeII RGCGCY 2 cut(s) 9, 393
HapII CCGG 2 cut(s) 357, 2170
HhaI GCGC 4 cut(s) 8, 392, 757, 2069
Hin6I GCGC 4 cut(s) 6, 390, 755, 2067
HinP1I GCGC 4 cut(s) 6, 390, 755, 2067
HincII GTYRAC 2 cut(s) 2573, 3265
HindII GTYRAC 2 cut(s) 2573, 3265
HindIII AAGCTT 2 cut(s) 1317, 2968
HinfI GANTC 9 cut(s) 478, 2237, 2371, 2553, 2814, 2947, 2960, 3079, 3169
HpaI GTTAAC 1 cut(s) 3265
HpaII CCGG 2 cut(s) 357, 2170
HphI GGTGA 5 cut(s) 194, 305, 1667, 2514, 3074
Hpy166II GTNNAC 8 cut(s) 1106, 1172, 2179, 2269, 2308, 2573, 3235, 3265
Hpy8I GTNNAC 8 cut(s) 1106, 1172, 2179, 2269, 2308, 2573, 3235, 3265
Hpy99I CGWCG 1 cut(s) 389
HpyCH4III ACNGT 5 cut(s) 520, 1570, 2279, 2515, 3155
HpyCH4IV ACGT 1 cut(s) 780
HpyF3I CTNAG 9 cut(s) 510, 1023, 1270, 1402, 1849, 2388, 2604, 2622, 3462
HpySE526I ACGT 1 cut(s) 780
HspAI GCGC 4 cut(s) 6, 390, 755, 2067
Kpn2I TCCGGA 1 cut(s) 2169
Ksp22I TGATCA 2 cut(s) 1779, 3367
KspAI GTTAAC 1 cut(s) 3265
LguI GCTCTTC 1 cut(s) 2697
LmnI GCTCC 5 cut(s) 226, 1144, 1763, 3089, 3437
Lsp1109I GCAGC 7 cut(s) 46, 769, 1391, 2200, 2222, 2507, 3175
MaeII ACGT 1 cut(s) 780
MaeIII GTNAC 6 cut(s) 1201, 1219, 1673, 1756, 1958, 2279
MbiI CCGCTC 1 cut(s) 1401
MboII GAAGA 9 cut(s) 109, 1156, 1195, 1946, 2168, 2492, 2684, 2760, 2946
MfeI CAATTG 3 cut(s) 471, 1239, 2934
MflI RGATCY 5 cut(s) 730, 982, 1005, 1195, 2391
MhlI GDGCHC 6 cut(s) 223, 266, 1067, 1289, 2600, 3077
MlsI TGGCCA 4 cut(s) 596, 683, 1299, 2060
MluNI TGGCCA 4 cut(s) 596, 683, 1299, 2060
MlyI GAGTC 4 cut(s) 487, 2246, 2547, 2969
MmeI TCCRAC 3 cut(s) 1489, 2922, 3195
Mox20I TGGCCA 4 cut(s) 596, 683, 1299, 2060
Mph1103I ATGCAT 2 cut(s) 1696, 1813
MroI TCCGGA 1 cut(s) 2169
MroXI GAANNNNTTC 3 cut(s) 651, 1974, 3014
MscI TGGCCA 4 cut(s) 596, 683, 1299, 2060
MslI CAYNNNNRTG 2 cut(s) 2033, 2414
Msp20I TGGCCA 4 cut(s) 596, 683, 1299, 2060
MspA1I CMGCKG 3 cut(s) 37, 760, 2213
MspCI CTTAAG 3 cut(s) 277, 1058, 1840
MspI CCGG 2 cut(s) 357, 2170
MspR9I CCNGG 2 cut(s) 171, 2264
MunI CAATTG 3 cut(s) 471, 1239, 2934
Mva1269I GAATGC 6 cut(s) 714, 794, 1811, 2072, 2802, 3292
MvaI CCWGG 2 cut(s) 171, 2264
MvnI CGCG 1 cut(s) 2069
NcoI CCATGG 1 cut(s) 642
NdeI CATATG 2 cut(s) 1804, 2529
NlaIV GGNNCC 8 cut(s) 264, 732, 854, 1146, 1197, 1902, 2014, 3091
NmeAIII GCCGAG 1 cut(s) 2572
NmuCI GTSAC 3 cut(s) 1219, 1673, 2279
NsiI ATGCAT 2 cut(s) 1696, 1813
NspI RCATGY 5 cut(s) 573, 630, 1694, 2326, 3281
OliI CACNNNNGTG 1 cut(s) 2414
PaeI GCATGC 2 cut(s) 573, 1694
PagI TCATGA 1 cut(s) 3006
PceI AGGCCT 1 cut(s) 29
PciI ACATGT 1 cut(s) 3277
PciSI GCTCTTC 1 cut(s) 2697
PctI GAATGC 6 cut(s) 714, 794, 1811, 2072, 2802, 3292
PdmI GAANNNNTTC 3 cut(s) 651, 1974, 3014
PfeI GAWTC 5 cut(s) 2371, 2814, 2947, 3079, 3169
PflMI CCANNNNNTGG 1 cut(s) 1295
PleI GAGTC 4 cut(s) 486, 2245, 2547, 2968
PpsI GAGTC 4 cut(s) 486, 2245, 2547, 2968
Ppu21I YACGTR 1 cut(s) 781
PscI ACATGT 1 cut(s) 3277
Psp124BI GAGCTC 1 cut(s) 2600
Psp6I CCWGG 2 cut(s) 169, 2262
PspGI CCWGG 2 cut(s) 169, 2262
PspN4I GGNNCC 8 cut(s) 264, 732, 854, 1146, 1197, 1902, 2014, 3091
PspOMI GGGCCC 1 cut(s) 262
PspPI GGNCC 6 cut(s) 262, 263, 852, 1106, 2013, 2533
PstNI CAGNNNCTG 1 cut(s) 1760
PsuI RGATCY 5 cut(s) 730, 982, 1005, 1195, 2391
PvuII CAGCTG 2 cut(s) 760, 2213
RsaI GTAC 7 cut(s) 1139, 1437, 1544, 2040, 2102, 2142, 2309
RsaNI GTAC 7 cut(s) 1138, 1436, 1543, 2039, 2101, 2141, 2308
RseI CAYNNNNRTG 2 cut(s) 2033, 2414
SacI GAGCTC 1 cut(s) 2600
SapI GCTCTTC 1 cut(s) 2697
Sau96I GGNCC 6 cut(s) 262, 263, 852, 1106, 2013, 2533
SchI GAGTC 4 cut(s) 487, 2246, 2547, 2969
ScrFI CCNGG 2 cut(s) 171, 2264
SduI GDGCHC 6 cut(s) 223, 266, 1067, 1289, 2600, 3077
SinI GGWCC 2 cut(s) 852, 1106
SmiMI CAYNNNNRTG 2 cut(s) 2033, 2414
SpeI ACTAGT 1 cut(s) 1894
SphI GCATGC 2 cut(s) 573, 1694
SseBI AGGCCT 1 cut(s) 29
SspI AATATT 3 cut(s) 1281, 1515, 2707
SstI GAGCTC 1 cut(s) 2600
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 2 cut(s) 169, 2262
StyI CCWWGG 4 cut(s) 65, 642, 1413, 1963
TaaI ACNGT 5 cut(s) 520, 1570, 2279, 2515, 3155
TaiI ACGT 1 cut(s) 783
TaqI TCGA 6 cut(s) 12, 118, 1002, 2235, 2592, 2975
TaqII GACCGA 1 cut(s) 840
TatI WGTACW 4 cut(s) 1137, 1542, 2140, 2307
TauI GCSGC 6 cut(s) 198, 201, 204, 1042, 2072, 3322
TfiI GAWTC 5 cut(s) 2371, 2814, 2947, 3079, 3169
TseFI GTSAC 3 cut(s) 1219, 1673, 2279
TseI GCWGC 7 cut(s) 34, 757, 1379, 2210, 2213, 2495, 3188
Tsp45I GTSAC 3 cut(s) 1219, 1673, 2279
Van91I CCANNNNNTGG 1 cut(s) 1295
Vha464I CTTAAG 3 cut(s) 277, 1058, 1840
VpaK11BI GGWCC 2 cut(s) 852, 1106
XagI CCTNNNNNAGG 1 cut(s) 1228
XapI RAATTY 4 cut(s) 395, 648, 2645, 2739
XceI RCATGY 5 cut(s) 573, 630, 1694, 2326, 3281
XcmI CCANNNNNNNNNTGG 4 cut(s) 63, 691, 2419, 3437
XmnI GAANNNNTTC 3 cut(s) 651, 1974, 3014
Zsp2I ATGCAT 2 cut(s) 1696, 1813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.