Prupe.1G349800_v2.0.a1
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010351
Physical Location & Seq
Reverse (-)
32601821 .. 32612483
10663 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G349800.1

Sequence Viewer

Length: 2403 bp
ATGTTAAAATTCTTGAAGGGAGTGGTGGGTGGATCGGGGACTGGACCCAAAGATCTGCCCTACAATATCGGCGAACCTTACCCCTCTGCTTGGGGCTCGTGGACTCACTTCCGGGGCACCTCCAAGGACGATGGGTCTCCGGTGTCCGTGTTTTCTATATCTGGAAGTAATGCTCAGGATGGCCATTTGGCCGCTGCTCGCAATGGTGTCAAGCGTCTCCGTACTGTCAGACATCCAAATATCTTATCCTTTCTTCATAGTACCGAGGCTGAAACTTTGGATGCTTCTACCACCAAGCAAACTATCTATATTGTTACCGAGCCTGTTATGCCACTCTCTGAAAAGATCAAGGAACTCAGTTTACAAGGTATCCAAAGGGATGAGTATTTCGCGTGGGGACTGCACCAGATTGCTAAAGCTGTCAGCTTTTTAAATAATGATTGTAAACTTGTTCATGCCAACGTTTGCTTGGCCAGTGTTGTTGTAACTCAAACTTTGGACTGGAAGCTGCATGCTTTTGATGTGCTATCTGAGTTTGATGGCAGCAATGAGGCTTCTGCTGGACAAATGCTGCAATTCGCCTGGCTTGTTGGACCACAATACAAACCCATGGAGTTGTTGAAGTCTGATTGGGCGGCAATCAGAAAGTCTCCACCGTGGGCCATTGATTCTTGGGGATTGGGTTGTCTTATCTATGAACTCTTCTCTGGTCTGAAGTTAAGCAAAACAGAGGAGCTGCGCAACACTGCTTCTATTCCAAAGTCTCTGCTTCCAGATTATCAGCGGCTCTTGAGCTCCACGCCTTCTCGTAGGCTGAATACGTCAAAGCTTATAGAAAATAGTGAGTATTTCCAAAATAAGCTGGTGGATACGATACATTTCATGGAAATTCTAAATCTGAAAGATAGTGTTGAGAAGGATACCTTCTTCCGCAAGCTTCCAAATTTAGCGGAGCAGCTACCTCGCCAAATTGTGCTGAAAAAGTTACTTCCTTTATTAGCTTCTGCTCTTGAATTTGGTTCAGCAGCTGCACCTGCTTTGACTGCATTGTTGAAAATGGGTGCCTGGCTTTCAACCGAAGAATTCAGTGTGAAGGTGCTGCCAACAATTGTGAAACTGTTTGCGTCCAATGACCGAGCTATTCGAGTTGGTCTCTTGCAACATGTCGATCAGTTTGGAGAATCATTAACAGCACAAGTTGTTGATGAGCAAGTTTACCCCCACGTTGCTACTGGGTTCTCTGACACATCTGCTTTTCTCCGGGAACTGACTCTTAAGTCGATGCTCGTTCTAGCTCCCAAGCTTTCTCAACGTACCATTTCAGGGTCTTTGTTGAAGTATCTTTCAAAGTTACAGGTTGATGAAGAACCAGCAATTAGAACAAACACCACTATATTGCTAGGGAACATTGCAACCCACCTGAATGATGGGACAAGGAAAAGAGTTTTGATTAATGCATTTACTGTCCGTGCATTACGTGATACTTTCTCACCTGCCCGAGGAGCAGGTATTATGGCTTTATGCGCCACCAGTTCTTATTATGACAGCACTGAGATTGCAACTCGGATTCTCCCAAATATTGTTGTTCTTACCATTGATCCCGACAATGATGTTCGCTCAAAGGCATTTCAAGCAGTTGATCAATTCCTTCAGATAGTGAAGCAATCCTATGAAAAGACAAATTCAGGAGATACTGCTGGGGCTGCTGGTCTCGGGATCTCATCAATACCAGGAAATGCTAGTTTACTGGGATGGGCAATGAGCTCCTTGACTCTCAAGGGTAAACCTTCTGAACAAGCTCCACTTGCTCCTGTGAATATCAGTACATCTCTTACCGAAACGACTTCCAATGCAAGCTCAGTGGTGGATACTCCAAGCACAGCAACTGCCCATGTAAGTACCACACCAGATTTTGCGGATCAACATGTACCTGAGTCCCCTACATCGACAGATGGCTGGGGAGAACTTGAAAATGGAATTGACGGAGAGCATGAAAGTGACAAGGATGGTTGGGATGATATTGAACCTCTGGAAGAGCCAAAACCATCTCCCGTTCTTGCAAGTATACAAGCAGCTCAAAAGCGGCCGGTCTCGCAACCTGTTTCGCAGCCTAAACAACAAGCAACAAGTTTGAGACCGAAAAACACAGCCAAGGCTATCAAAAATGAAGATGATGATTTGTGGGGCTCCATTGCCGCCCCGGCACCCAAAACAATTTCAAAACCTTTGAATTTAAAAACAAGTGGAGCCGTTGATGATGATGATCCATGGGCTGCCATCGCTGCTCCCCAACCTACCACCAAGGCAAAGCCCCTAGCAGCTGTGAAAGGTCGAGGAACCAAACCTGCTGCTCCAAAACTGGGTGCACAGAGAATAAACCGGACGTCTTCATCTGGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

801

Amino Acids

86.63

Weight (kDa)

6.73

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1042, 1501
AasI GACNNNNNNGTC 1 cut(s) 1276
AatII GACGTC 1 cut(s) 2387
Acc16I TGCGCA 1 cut(s) 740
Acc36I ACCTGC 4 cut(s) 1042, 1496, 1501, 2353
AccB1I GGYRCC 3 cut(s) 116, 1061, 2203
AccI GTMKAC 1 cut(s) 2065
AccII CGCG 1 cut(s) 392
AciI CCGC 8 cut(s) 192, 635, 784, 931, 950, 1916, 2083, 2196
AclI AACGTT 1 cut(s) 462
AclWI GGATC 5 cut(s) 40, 1592, 1724, 1926, 2258
AcoI YGGCCR 4 cut(s) 181, 189, 471, 2084
AcsI RAATTY 7 cut(s) 8, 888, 943, 1013, 1082, 1681, 2230
AcuI CTGAAG 2 cut(s) 734, 1634
AcyI GRCGYC 1 cut(s) 2384
AfaI GTAC 6 cut(s) 223, 262, 1315, 1825, 1900, 1929
AfiI CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2360
AflII CTTAAG 1 cut(s) 1274
AflIII ACRYGT 2 cut(s) 1162, 1924
AhdI GACNNNNNGTC 1 cut(s) 133
AjnI CCWGG 3 cut(s) 581, 1064, 1729
Alw21I GWGCWC 3 cut(s) 797, 1766, 2368
Alw26I GTCTC 8 cut(s) 141, 221, 654, 768, 1157, 1715, 2095, 2128
Alw44I GTGCAC 1 cut(s) 2364
AlwI GGATC 5 cut(s) 40, 1592, 1724, 1926, 2258
AlwNI CAGNNNCTG 2 cut(s) 1028, 1886
Ama87I CYCGRG 2 cut(s) 1497, 1712
AoxI GGCC 5 cut(s) 181, 189, 471, 660, 2084
ApaLI GTGCAC 1 cut(s) 2364
ApoI RAATTY 7 cut(s) 8, 888, 943, 1013, 1082, 1681, 2230
AseI ATTAAT 1 cut(s) 1452
AspLEI GCGC 2 cut(s) 741, 1526
AspS9I GGNCC 3 cut(s) 44, 593, 660
AsuC2I CCSGG 3 cut(s) 113, 1262, 2201
AsuHPI GGTGA 1 cut(s) 1482
AvaI CYCGRG 2 cut(s) 1497, 1712
AvaII GGWCC 2 cut(s) 44, 593
BaeGI GKGCMC 2 cut(s) 119, 2368
BalI TGGCCA 2 cut(s) 183, 473
BanI GGYRCC 3 cut(s) 116, 1061, 2203
BanII GRGCYC 4 cut(s) 98, 797, 1766, 2189
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 2379
Bbv12I GWGCWC 3 cut(s) 797, 1766, 2368
BccI CCATC 9 cut(s) 125, 173, 533, 1421, 1746, 1946, 2000, 2053, 2285
BceAI ACGGC 1 cut(s) 2234
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 3 cut(s) 583, 1066, 1731
BciVI GTATCC 4 cut(s) 380, 862, 913, 1861
BclI TGATCA 1 cut(s) 1639
BcnI CCSGG 3 cut(s) 113, 1262, 2201
BcoDI GTCTC 8 cut(s) 141, 221, 654, 768, 1157, 1715, 2095, 2128
BfaI CTAG 4 cut(s) 1292, 1400, 1740, 2315
BfrI CTTAAG 1 cut(s) 1274
BfuAI ACCTGC 4 cut(s) 1042, 1496, 1501, 2353
BfuI GTATCC 4 cut(s) 380, 862, 913, 1861
BglI GCCNNNNNGGC 1 cut(s) 2201
BglII AGATCT 1 cut(s) 52
Bme1390I CCNGG 6 cut(s) 113, 583, 1066, 1262, 1731, 2201
Bme18I GGWCC 2 cut(s) 44, 593
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 1497, 1712
BmgT120I GGNCC 3 cut(s) 44, 593, 660
BmiI GGNNCC 7 cut(s) 46, 118, 1063, 2188, 2205, 2248, 2338
BmrFI CCNGG 6 cut(s) 113, 583, 1066, 1262, 1731, 2201
BmrI ACTGGG 3 cut(s) 1242, 1757, 2369
BmsI GCATC 2 cut(s) 271, 1272
BmuI ACTGGG 3 cut(s) 1242, 1757, 2369
BpiI GAAGAC 1 cut(s) 2379
BplI GAGNNNNNCTC 2 cut(s) 1137, 1169
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 811, 1760
BpuMI CCSGG 3 cut(s) 113, 1262, 2201
BsaAI YACGTR 1 cut(s) 1478
BsaBI GATNNNNATC 1 cut(s) 2262
BsaHI GRCGYC 1 cut(s) 2384
BsaI GGTCTC 5 cut(s) 141, 1157, 1715, 2095, 2128
BsaWI WCCGGW 2 cut(s) 139, 2379
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2360
Bse118I RCCGGY 1 cut(s) 2086
Bse1I ACTGG 7 cut(s) 46, 474, 506, 1237, 1530, 1752, 2364
Bse3DI GCAATG 5 cut(s) 208, 553, 1407, 1764, 2190
Bse8I GATNNNNATC 1 cut(s) 2262
BseBI CCWGG 3 cut(s) 583, 1066, 1731
BseGI GGATG 7 cut(s) 184, 232, 286, 385, 1757, 2011, 2020
BseJI GATNNNNATC 1 cut(s) 2262
BseLI CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2360
BseMI GCAATG 5 cut(s) 208, 553, 1407, 1764, 2190
BseMII CTCAG 6 cut(s) 188, 370, 522, 1542, 1872, 1923
BseNI ACTGG 7 cut(s) 46, 474, 506, 1237, 1530, 1752, 2364
BseRI GAGGAG 2 cut(s) 746, 1515
BseSI GKGCMC 2 cut(s) 119, 2368
BseX3I CGGCCG 1 cut(s) 2084
BseYI CCCAGC 2 cut(s) 1697, 1956
BsgI GTGCAG 2 cut(s) 386, 1014
Bsh1236I CGCG 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 2087
BshFI GGCC 5 cut(s) 183, 191, 473, 662, 2086
BshNI GGYRCC 3 cut(s) 116, 1061, 2203
BsiEI CGRYCG 1 cut(s) 2087
BsiHKAI GWGCWC 3 cut(s) 797, 1766, 2368
BsiHKCI CYCGRG 2 cut(s) 1497, 1712
BsiSI CCGG 6 cut(s) 112, 140, 1261, 2087, 2201, 2380
BslFI GGGAC 4 cut(s) 52, 411, 1444, 1921
BslI CCNNNNNNNGG 4 cut(s) 90, 1323, 1499, 2360
BsmAI GTCTC 8 cut(s) 141, 221, 654, 768, 1157, 1715, 2095, 2128
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 4 cut(s) 52, 411, 1444, 1921
BsnI GGCC 5 cut(s) 183, 191, 473, 662, 2086
Bso31I GGTCTC 5 cut(s) 141, 1157, 1715, 2095, 2128
BsoBI CYCGRG 2 cut(s) 1497, 1712
Bsp1286I GDGCHC 6 cut(s) 98, 119, 797, 1766, 2189, 2368
Bsp143I GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
Bsp19I CCATGG 2 cut(s) 609, 2267
BspACI CCGC 8 cut(s) 192, 635, 784, 931, 950, 1916, 2083, 2196
BspANI GGCC 5 cut(s) 183, 191, 473, 662, 2086
BspCNI CTCAG 6 cut(s) 187, 369, 523, 1543, 1871, 1924
BspFNI CGCG 1 cut(s) 392
BspLI GGNNCC 7 cut(s) 46, 118, 1063, 2188, 2205, 2248, 2338
BspMI ACCTGC 4 cut(s) 1042, 1496, 1501, 2353
BspPI GGATC 5 cut(s) 40, 1592, 1724, 1926, 2258
BspQI GCTCTTC 1 cut(s) 2028
BspT107I GGYRCC 3 cut(s) 116, 1061, 2203
BspTI CTTAAG 1 cut(s) 1274
BspTNI GGTCTC 5 cut(s) 141, 1157, 1715, 2095, 2128
BsrDI GCAATG 5 cut(s) 208, 553, 1407, 1764, 2190
BsrFI RCCGGY 1 cut(s) 2086
BsrI ACTGG 7 cut(s) 46, 474, 506, 1237, 1530, 1752, 2364
BssAI RCCGGY 1 cut(s) 2086
BssMI GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
BssNAI GTATAC 1 cut(s) 2066
BssNI GRCGYC 1 cut(s) 2384
BssSI CACGAG 1 cut(s) 97
BssT1I CCWWGG 5 cut(s) 123, 609, 2151, 2267, 2301
Bst1107I GTATAC 1 cut(s) 2066
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 3 cut(s) 583, 1066, 1731
Bst4CI ACNGT 4 cut(s) 226, 657, 1119, 1465
Bst6I CTCTTC 2 cut(s) 707, 2028
BstACI GRCGYC 1 cut(s) 2384
BstAFI CTTAAG 1 cut(s) 1274
BstBAI YACGTR 1 cut(s) 1478
BstC8I GCNNGC 4 cut(s) 199, 513, 935, 1855
BstDEI CTNAG 6 cut(s) 174, 356, 531, 1551, 1858, 1932
BstDSI CCRYGG 3 cut(s) 609, 656, 2267
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 7 cut(s) 184, 232, 286, 385, 1757, 2011, 2020
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 2 cut(s) 741, 1526
BstKTI GATC 9 cut(s) 35, 55, 348, 1171, 1600, 1642, 1719, 1921, 2266
BstMAI GTCTC 8 cut(s) 141, 221, 654, 768, 1157, 1715, 2095, 2128
BstMBI GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
BstMCI CGRYCG 1 cut(s) 2087
BstNI CCWGG 3 cut(s) 583, 1066, 1731
BstNSI RCATGY 3 cut(s) 515, 1166, 1928
BstSCI CCNGG 6 cut(s) 111, 581, 1064, 1260, 1729, 2199
BstSLI GKGCMC 2 cut(s) 119, 2368
BstUI CGCG 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 2379
BstX2I RGATCY 2 cut(s) 52, 1716
BstYI RGATCY 2 cut(s) 52, 1716
BstZ17I GTATAC 1 cut(s) 2066
BstZI CGGCCG 1 cut(s) 2084
BsuI GTATCC 4 cut(s) 380, 862, 913, 1861
BsuRI GGCC 5 cut(s) 183, 191, 473, 662, 2086
BtgI CCRYGG 3 cut(s) 609, 656, 2267
BtgZI GCGATG 1 cut(s) 2263
BtsCI GGATG 7 cut(s) 184, 232, 286, 385, 1757, 2011, 2020
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 5 cut(s) 481, 744, 1093, 1548, 1866
BveI ACCTGC 4 cut(s) 1042, 1496, 1501, 2353
Cac8I GCNNGC 4 cut(s) 199, 513, 935, 1855
CaiI CAGNNNCTG 2 cut(s) 1028, 1886
CfoI GCGC 2 cut(s) 741, 1526
Cfr10I RCCGGY 1 cut(s) 2086
Cfr13I GGNCC 3 cut(s) 44, 593, 660
CseI GACGC 2 cut(s) 203, 1113
Csp6I GTAC 6 cut(s) 222, 261, 1314, 1824, 1899, 1928
CspCI CAANNNNNGTGG 2 cut(s) 1842, 1877
CviAII CATG 9 cut(s) 455, 512, 610, 883, 1163, 1892, 1925, 1991, 2268
CviQI GTAC 6 cut(s) 222, 261, 1314, 1824, 1899, 1928
DdeI CTNAG 6 cut(s) 174, 356, 531, 1551, 1858, 1932
DpnI GATC 9 cut(s) 34, 54, 347, 1170, 1599, 1641, 1718, 1920, 2265
DpnII GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
DraI TTTAAA 2 cut(s) 432, 2235
DrdI GACNNNNNNGTC 1 cut(s) 1276
DriI GACNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 1276
EaeI YGGCCR 4 cut(s) 181, 189, 471, 2084
EagI CGGCCG 1 cut(s) 2084
Eam1104I CTCTTC 2 cut(s) 707, 2028
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 2 cut(s) 707, 2028
Ecl136II GAGCTC 2 cut(s) 795, 1764
EclXI CGGCCG 1 cut(s) 2084
Eco130I CCWWGG 5 cut(s) 123, 609, 2151, 2267, 2301
Eco24I GRGCYC 4 cut(s) 98, 797, 1766, 2189
Eco31I GGTCTC 5 cut(s) 141, 1157, 1715, 2095, 2128
Eco47I GGWCC 2 cut(s) 44, 593
Eco52I CGGCCG 1 cut(s) 2084
Eco53kI GAGCTC 2 cut(s) 795, 1764
Eco57I CTGAAG 2 cut(s) 734, 1634
Eco88I CYCGRG 2 cut(s) 1497, 1712
EcoICRI GAGCTC 2 cut(s) 795, 1764
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoRI GAATTC 1 cut(s) 1082
EcoRII CCWGG 3 cut(s) 581, 1064, 1729
EcoT14I CCWWGG 5 cut(s) 123, 609, 2151, 2267, 2301
EcoT22I ATGCAT 1 cut(s) 1459
EcoT38I GRGCYC 4 cut(s) 98, 797, 1766, 2189
ErhI CCWWGG 5 cut(s) 123, 609, 2151, 2267, 2301
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 9 cut(s) 458, 515, 613, 886, 1166, 1895, 1928, 1994, 2271
FalI AAGNNNNNCTT 4 cut(s) 908, 940, 1788, 1820
FaqI GGGAC 4 cut(s) 52, 411, 1444, 1921
FatI CATG 9 cut(s) 454, 511, 609, 882, 1162, 1891, 1924, 1990, 2267
FbaI TGATCA 1 cut(s) 1639
FblI GTMKAC 1 cut(s) 2065
FokI GGATG 7 cut(s) 191, 219, 293, 392, 1764, 2018, 2027
FriOI GRGCYC 4 cut(s) 98, 797, 1766, 2189
FspBI CTAG 4 cut(s) 1292, 1400, 1740, 2315
FspI TGCGCA 1 cut(s) 740
GlaI GCGC 2 cut(s) 740, 1525
GsaI CCCAGC 2 cut(s) 1701, 1960
HaeIII GGCC 5 cut(s) 183, 191, 473, 662, 2086
HapII CCGG 6 cut(s) 112, 140, 1261, 2087, 2201, 2380
HgaI GACGC 2 cut(s) 203, 1113
HhaI GCGC 2 cut(s) 741, 1526
Hin1I GRCGYC 1 cut(s) 2384
Hin1II CATG 9 cut(s) 458, 515, 613, 886, 1166, 1895, 1928, 1994, 2271
Hin6I GCGC 2 cut(s) 739, 1524
HinP1I GCGC 2 cut(s) 739, 1524
HindIII AAGCTT 3 cut(s) 827, 935, 1301
HinfI GANTC 7 cut(s) 103, 668, 1181, 1270, 1567, 1771, 1934
HpaII CCGG 6 cut(s) 112, 140, 1261, 2087, 2201, 2380
HphI GGTGA 1 cut(s) 1482
Hpy166II GTNNAC 8 cut(s) 102, 362, 446, 1216, 1745, 1784, 2066, 2366
Hpy8I GTNNAC 8 cut(s) 102, 362, 446, 1216, 1745, 1784, 2066, 2366
HpyAV CCTTC 7 cut(s) 10, 813, 910, 934, 1087, 1658, 1797
HpyCH4III ACNGT 4 cut(s) 226, 657, 1119, 1465
HpyCH4IV ACGT 6 cut(s) 462, 821, 1224, 1312, 1477, 2384
HpyF3I CTNAG 6 cut(s) 174, 356, 531, 1551, 1858, 1932
HpySE526I ACGT 6 cut(s) 462, 821, 1224, 1312, 1477, 2384
Hsp92I GRCGYC 1 cut(s) 2384
Hsp92II CATG 9 cut(s) 458, 515, 613, 886, 1166, 1895, 1928, 1994, 2271
HspAI GCGC 2 cut(s) 739, 1524
Ksp22I TGATCA 1 cut(s) 1639
Kzo9I GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
LguI GCTCTTC 1 cut(s) 2028
LweI GCATC 2 cut(s) 271, 1272
MaeI CTAG 4 cut(s) 1292, 1400, 1740, 2315
MaeII ACGT 6 cut(s) 462, 821, 1224, 1312, 1477, 2384
MaeIII GTNAC 5 cut(s) 313, 484, 984, 1350, 1997
MalI GATC 9 cut(s) 34, 54, 347, 1170, 1599, 1641, 1718, 1920, 2265
MboI GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
MboII GAAGA 8 cut(s) 245, 694, 919, 1091, 1376, 2045, 2180, 2379
MfeI CAATTG 1 cut(s) 1107
MflI RGATCY 2 cut(s) 52, 1716
MhlI GDGCHC 6 cut(s) 98, 119, 797, 1766, 2189, 2368
MlsI TGGCCA 2 cut(s) 183, 473
MluNI TGGCCA 2 cut(s) 183, 473
MlyI GAGTC 4 cut(s) 97, 1264, 1765, 1943
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 9 cut(s) 94, 130, 259, 544, 724, 972, 1493, 2037, 2327
Mox20I TGGCCA 2 cut(s) 183, 473
Mph1103I ATGCAT 1 cut(s) 1459
MscI TGGCCA 2 cut(s) 183, 473
MseI TTAA 7 cut(s) 5, 431, 719, 1187, 1275, 1452, 2234
MslI CAYNNNNRTG 2 cut(s) 1422, 1995
Msp20I TGGCCA 2 cut(s) 183, 473
MspA1I CMGCKG 4 cut(s) 194, 784, 1028, 2321
MspCI CTTAAG 1 cut(s) 1274
MspI CCGG 6 cut(s) 112, 140, 1261, 2087, 2201, 2380
MspR9I CCNGG 6 cut(s) 113, 583, 1066, 1262, 1731, 2201
MunI CAATTG 1 cut(s) 1107
MvaI CCWGG 3 cut(s) 583, 1066, 1731
MvnI CGCG 1 cut(s) 392
NciI CCSGG 3 cut(s) 113, 1262, 2201
NcoI CCATGG 2 cut(s) 609, 2267
NdeII GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
NlaIII CATG 9 cut(s) 458, 515, 613, 886, 1166, 1895, 1928, 1994, 2271
NlaIV GGNNCC 7 cut(s) 46, 118, 1063, 2188, 2205, 2248, 2338
NmuCI GTSAC 1 cut(s) 1997
NsbI TGCGCA 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 1459
NspI RCATGY 3 cut(s) 515, 1166, 1928
PaeI GCATGC 1 cut(s) 515
PaqCI CACCTGC 2 cut(s) 1042, 1501
PciI ACATGT 2 cut(s) 1162, 1924
PciSI GCTCTTC 1 cut(s) 2028
PfeI GAWTC 3 cut(s) 668, 1181, 1567
PfoI TCCNGGA 1 cut(s) 1260
PleI GAGTC 4 cut(s) 97, 1264, 1765, 1942
PpsI GAGTC 4 cut(s) 97, 1264, 1765, 1942
Ppu21I YACGTR 1 cut(s) 1478
PscI ACATGT 2 cut(s) 1162, 1924
PshBI ATTAAT 1 cut(s) 1452
Psp124BI GAGCTC 2 cut(s) 797, 1766
Psp1406I AACGTT 1 cut(s) 462
Psp6I CCWGG 3 cut(s) 581, 1064, 1729
PspFI CCCAGC 2 cut(s) 1697, 1956
PspGI CCWGG 3 cut(s) 581, 1064, 1729
PspN4I GGNNCC 7 cut(s) 46, 118, 1063, 2188, 2205, 2248, 2338
PspPI GGNCC 3 cut(s) 44, 593, 660
PsrI GAACNNNNNNTAC 2 cut(s) 345, 377
PstNI CAGNNNCTG 2 cut(s) 1028, 1886
PsuI RGATCY 2 cut(s) 52, 1716
PvuII CAGCTG 2 cut(s) 1028, 2321
RsaI GTAC 6 cut(s) 223, 262, 1315, 1825, 1900, 1929
RsaNI GTAC 6 cut(s) 222, 261, 1314, 1824, 1899, 1928
RseI CAYNNNNRTG 2 cut(s) 1422, 1995
SacI GAGCTC 2 cut(s) 797, 1766
SapI GCTCTTC 1 cut(s) 2028
SaqAI TTAA 7 cut(s) 5, 431, 719, 1187, 1275, 1452, 2234
Sau3AI GATC 9 cut(s) 32, 52, 345, 1168, 1597, 1639, 1716, 1918, 2263
Sau96I GGNCC 3 cut(s) 44, 593, 660
SchI GAGTC 4 cut(s) 97, 1264, 1765, 1943
ScrFI CCNGG 6 cut(s) 113, 583, 1066, 1262, 1731, 2201
SduI GDGCHC 6 cut(s) 98, 119, 797, 1766, 2189, 2368
SfaNI GCATC 2 cut(s) 271, 1272
SinI GGWCC 2 cut(s) 44, 593
SmiMI CAYNNNNRTG 2 cut(s) 1422, 1995
SmlI CTYRAG 3 cut(s) 790, 1274, 1775
SmoI CTYRAG 3 cut(s) 790, 1274, 1775
SphI GCATGC 1 cut(s) 515
SsiI CCGC 8 cut(s) 192, 635, 784, 931, 950, 1916, 2083, 2196
SspI AATATT 1 cut(s) 1579
SspMI CTAG 4 cut(s) 1292, 1400, 1740, 2315
SstI GAGCTC 2 cut(s) 797, 1766
StyD4I CCNGG 6 cut(s) 111, 581, 1064, 1260, 1729, 2199
StyI CCWWGG 5 cut(s) 123, 609, 2151, 2267, 2301
TaaI ACNGT 4 cut(s) 226, 657, 1119, 1465
TaiI ACGT 6 cut(s) 465, 824, 1227, 1315, 1480, 2387
TaqI TCGA 5 cut(s) 1144, 1167, 1280, 1946, 2332
TaqII GACCGA 2 cut(s) 1149, 2152
TatI WGTACW 1 cut(s) 1823
TauI GCSGC 5 cut(s) 194, 638, 787, 2086, 2198
TfiI GAWTC 3 cut(s) 668, 1181, 1567
Tru1I TTAA 7 cut(s) 5, 431, 719, 1187, 1275, 1452, 2234
Tru9I TTAA 7 cut(s) 5, 431, 719, 1187, 1275, 1452, 2234
TscAI CASTG 5 cut(s) 481, 751, 1093, 1555, 1866
TseFI GTSAC 1 cut(s) 1997
Tsp45I GTSAC 1 cut(s) 1997
TspDTI ATGAA 9 cut(s) 245, 443, 711, 871, 1377, 1686, 2007, 2181, 2379
TspGWI ACGGA 4 cut(s) 136, 209, 1457, 1998
TspRI CASTG 5 cut(s) 481, 751, 1093, 1555, 1866
Vha464I CTTAAG 1 cut(s) 1274
VneI GTGCAC 1 cut(s) 2364
VpaK11BI GGWCC 2 cut(s) 44, 593
VspI ATTAAT 1 cut(s) 1452
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 7 cut(s) 8, 888, 943, 1013, 1082, 1681, 2230
XceI RCATGY 3 cut(s) 515, 1166, 1928
XcmI CCANNNNNNNNNTGG 3 cut(s) 466, 1229, 1424
XmiI GTMKAC 1 cut(s) 2065
XspI CTAG 4 cut(s) 1292, 1400, 1740, 2315
ZraI GACGTC 1 cut(s) 2385
Zsp2I ATGCAT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.