Rorug04G0377000
NAC Family

inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010351
Physical Location & Seq
Forward (+)
54769974 .. 54772584
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0377000.1

Sequence Viewer

Length: 1362 bp
ATGACCACCGGCGACGAAAAGACCGAGTCTTTACCGGAATCCAAGCTACCCGGTAACGAGAACAGTCCACTGCTGCAAAACCCAGATGAGGAAATCAACGGCGGGTCGTATTCCGGCGCGGTGTTCAATCTGTCGACGACGATTATTGGGGCCGGGATCATGGCTTTGCCGGCGACGATGAAGGTGCTGGGCCTCGGCGTCGGGATTGGCATGATCGTGTTGGTTGCAGTTTTGACTGAGGCCGCCGTGGAGATGTTGCTCAGGTTCAGTAGGGCCGGCAATGCGGCGTCGTTTGGTGGTGTTATGGGGGATGCTTTTGGAAGAGGTGGCAAGGTCGTGTTTCAGCTGTGTATCTTGGTCAACAATGTTGGGACTCTCATTGTTTACATGATCATACTTGGCGATGTGCTGTCTGGAACTGCTTCGAGTGGAGCTCACCATACTGGAGTGTTGGAAGGGTGGTTTGGTGAAAACTGGTGGAATGGGCGTCTTTTCGTTCTGTTTGTTTCAACGATTTTCGTGTTTGCTCCGTTGGTATCCCTGAAGCGAATAGATTCATTGAGATACTCATCTACTTTAGCAGTTGGGCTGGCAGGCGTTTTTCTAGTCATTACTGCAGGAATCACAGCTGTTAAAATTTCTCATGGAGATATTTCCATGCCCAGGTTGCTTCCTGTGTTGACTGATCTTACGTCAGTGTGGAATCTCTTCACAGTTGTTCCTGTTCTTGTGACGGCCTTCATCTGCCACTACAATGTGCATACGATCAACAATGAGCTTAAGAAGTCTAGCATGATTCAACCAGTTGTGCGGACATCATTAATATTGTGCTCTTCTGTCTACATACTGACCAGCTTTTTCGGGTTCCTCCTATTTGGGGACTCGACCCTTGATGATGTGTTAGCCAACTTTGACACCGACCTTGGCATCCCTTACAGCTCTTTGCTCAGTGATATTGTCCGCATCAGCTATGCCCTGCACCTCATGCTTGTTTTTCCTATTATCTTTCATCCACTGCGAATCAACTTCGATGACCTCTTCTTTCCTTCATCTAGGCCTTTGGATTTAGATAACCGTAGGTTTGCATTAATCACCTTCGGACTCATTTCTGTTATCTTCTTGGGCGCAAACTTCATTCCAAGCATCTGGGACGTTTTCCAGTTTACTGGAGCGACTGCCACAGTTTGCATTGGATTTATCTTCCCTGCTGCCATTGTTCTAAGGGACCGGCATGGCATAGCAACATCAAAGGACAAGACCTTATCTGTTTTCATGATTGGCCTTGCTGTGTTCGCAAATTTGATAGCAATATACAGTGATGCCGTGGCCTTGTTCAAAAAGAATGTCTCCCCTCAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

453

Amino Acids

48.82

Weight (kDa)

5.89

Isoelectric Point (pI)

29.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 36 - 439 6e-55 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40730
fragaria_vesca FvH4_4g37220 FvH4_4g37220 FvH4_4g37220 FvH4_4g37220
malus_domestica MD00G1126900.v1.1 MD13G1002600.v1.1
prunus_persica Prupe.1G349800_v2.0.a1
pyrus_communis pycom13g00220 pycom16g00140
rosa_chinensis RchiOBHm_Chr4g0447001
rosa_laevigata RLG00000005613
rosa_multiflora Rmu_sc0001753.1_g000002
rosa_roxburghii Rroxscaffold_5G00387330
rosa_rugosa Rorug04G0376900 Rorug04G0377000
rosa_samantha Rh4AG438500 Rh4BG447500 Rh4CG464600 Rh4DG446100
rosa_wichuraiana Rw4G037500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 134, 840
AccII CGCG 1 cut(s) 119
AciI CCGC 6 cut(s) 102, 119, 243, 284, 811, 961
AclWI GGATC 1 cut(s) 164
AcsI RAATTY 2 cut(s) 636, 1297
AcuI CTGAAG 1 cut(s) 563
AcyI GRCGYC 3 cut(s) 198, 287, 487
AfiI CCNNNNNNNGG 3 cut(s) 88, 663, 877
AflII CTTAAG 1 cut(s) 779
AgsI TTSAA 4 cut(s) 127, 510, 800, 1336
AjnI CCWGG 1 cut(s) 662
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
AluBI AGCT 8 cut(s) 46, 346, 434, 629, 778, 855, 939, 969
AluI AGCT 8 cut(s) 46, 346, 434, 629, 778, 855, 939, 969
Alw21I GWGCWC 2 cut(s) 436, 833
Alw26I GTCTC 1 cut(s) 1351
AlwI GGATC 1 cut(s) 164
AoxI GGCC 8 cut(s) 150, 190, 240, 273, 735, 1055, 1279, 1326
ApeKI GCWGC 2 cut(s) 73, 1208
ApoI RAATTY 2 cut(s) 636, 1297
ArsI GACNNNNNNTTYG 2 cut(s) 1329, 1361
AseI ATTAAT 2 cut(s) 821, 1088
Asp700I GAANNNNTTC 3 cut(s) 421, 553, 707
AspLEI GCGC 2 cut(s) 119, 1127
AspS9I GGNCC 4 cut(s) 150, 190, 273, 1225
AsuC2I CCSGG 2 cut(s) 51, 154
AsuHPI GGTGA 3 cut(s) 428, 479, 1084
AvaII GGWCC 1 cut(s) 1225
BanII GRGCYC 1 cut(s) 436
Bbv12I GWGCWC 2 cut(s) 436, 833
BbvI GCAGC 2 cut(s) 60, 1195
BceAI ACGGC 4 cut(s) 115, 230, 750, 1307
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 664
BciVI GTATCC 1 cut(s) 547
BclI TGATCA 1 cut(s) 390
BcnI CCSGG 2 cut(s) 51, 154
BcoDI GTCTC 1 cut(s) 1351
BfaI CTAG 3 cut(s) 605, 789, 1053
BfmI CTRYAG 1 cut(s) 615
BfrI CTTAAG 1 cut(s) 779
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 4 cut(s) 74, 243, 285, 1209
BlsI GCNGC 4 cut(s) 75, 244, 286, 1210
Bme1390I CCNGG 3 cut(s) 51, 154, 664
Bme18I GGWCC 1 cut(s) 1225
BmgT120I GGNCC 4 cut(s) 150, 190, 273, 1225
BmiI GGNNCC 3 cut(s) 151, 866, 1226
BmrFI CCNGG 3 cut(s) 51, 154, 664
BmsI GCATC 5 cut(s) 301, 936, 972, 1152, 1309
BplI GAGNNNNNCTC 2 cut(s) 418, 450
BpmI CTGGAG 2 cut(s) 465, 1188
Bpu10I CCTNAGC 1 cut(s) 260
BpuEI CTTGAG 1 cut(s) 1338
BpuMI CCSGG 2 cut(s) 51, 154
BsaBI GATNNNNATC 1 cut(s) 568
BsaHI GRCGYC 3 cut(s) 198, 287, 487
BsaJI CCNNGG 5 cut(s) 193, 246, 662, 922, 1323
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 663, 877
Bse118I RCCGGY 4 cut(s) 8, 169, 275, 1227
Bse1I ACTGG 5 cut(s) 448, 479, 803, 1159, 1171
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 568
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 5 cut(s) 193, 246, 662, 922, 1323
BseGI GGATG 3 cut(s) 316, 927, 1009
BseJI GATNNNNATC 1 cut(s) 568
BseLI CCNNNNNNNGG 3 cut(s) 88, 663, 877
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 228, 274, 961
BseNI ACTGG 5 cut(s) 448, 479, 803, 1159, 1171
BseXI GCAGC 2 cut(s) 60, 1195
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 1 cut(s) 962
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 8 cut(s) 152, 192, 242, 275, 737, 1057, 1281, 1328
BsiHKAI GWGCWC 2 cut(s) 436, 833
BsiSI CCGG 8 cut(s) 9, 35, 51, 114, 153, 170, 276, 1228
BslFI GGGAC 4 cut(s) 385, 893, 1163, 1238
BslI CCNNNNNNNGG 3 cut(s) 88, 663, 877
BsmAI GTCTC 1 cut(s) 1351
BsmFI GGGAC 4 cut(s) 385, 893, 1163, 1238
BsnI GGCC 8 cut(s) 152, 192, 242, 275, 737, 1057, 1281, 1328
Bsp1286I GDGCHC 2 cut(s) 436, 833
Bsp143I GATC 5 cut(s) 156, 213, 390, 685, 765
BspACI CCGC 6 cut(s) 102, 119, 243, 284, 811, 961
BspANI GGCC 8 cut(s) 152, 192, 242, 275, 737, 1057, 1281, 1328
BspCNI CTCAG 3 cut(s) 229, 273, 960
BspFNI CGCG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 1272
BspLI GGNNCC 3 cut(s) 151, 866, 1226
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 1 cut(s) 164
BspQI GCTCTTC 1 cut(s) 838
BspTI CTTAAG 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 4 cut(s) 8, 169, 275, 1227
BsrI ACTGG 5 cut(s) 448, 479, 803, 1159, 1171
BssAI RCCGGY 4 cut(s) 8, 169, 275, 1227
BssECI CCNNGG 5 cut(s) 193, 246, 662, 922, 1323
BssMI GATC 5 cut(s) 156, 213, 390, 685, 765
BssNI GRCGYC 3 cut(s) 198, 287, 487
BssT1I CCWWGG 1 cut(s) 922
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 5 cut(s) 65, 715, 1076, 1183, 1316
Bst6I CTCTTC 4 cut(s) 316, 713, 838, 1043
BstACI GRCGYC 3 cut(s) 198, 287, 487
BstAFI CTTAAG 1 cut(s) 779
BstAPI GCANNNNNTGC 1 cut(s) 985
BstC8I GCNNGC 4 cut(s) 171, 277, 591, 595
BstDEI CTNAG 4 cut(s) 237, 260, 947, 1220
BstDSI CCRYGG 2 cut(s) 246, 1323
BstF5I GGATG 3 cut(s) 316, 927, 1009
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 2 cut(s) 119, 1127
BstKTI GATC 5 cut(s) 159, 216, 393, 688, 768
BstMAI GTCTC 1 cut(s) 1351
BstMBI GATC 5 cut(s) 156, 213, 390, 685, 765
BstMWI GCNNNNNNNGC 5 cut(s) 170, 281, 667, 985, 1292
BstNI CCWGG 1 cut(s) 664
BstSCI CCNGG 3 cut(s) 49, 152, 662
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 2 cut(s) 60, 1195
BstXI CCANNNNNNTGG 2 cut(s) 1146, 1166
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 8 cut(s) 152, 192, 242, 275, 737, 1057, 1281, 1328
BtgI CCRYGG 2 cut(s) 246, 1323
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 316, 927, 1009
BtsI GCAGTG 2 cut(s) 68, 1013
BtsIMutI CAGTG 5 cut(s) 68, 702, 955, 1013, 1321
Cac8I GCNNGC 4 cut(s) 171, 277, 591, 595
CciI TCATGA 1 cut(s) 1272
CfoI GCGC 2 cut(s) 119, 1127
Cfr10I RCCGGY 4 cut(s) 8, 169, 275, 1227
Cfr13I GGNCC 4 cut(s) 150, 190, 273, 1225
CseI GACGC 3 cut(s) 187, 276, 476
CviAII CATG 9 cut(s) 160, 211, 388, 644, 658, 793, 985, 1232, 1273
DdeI CTNAG 4 cut(s) 237, 260, 947, 1220
DpnI GATC 5 cut(s) 158, 215, 392, 687, 767
DpnII GATC 5 cut(s) 156, 213, 390, 685, 765
Eam1104I CTCTTC 4 cut(s) 316, 713, 838, 1043
EarI CTCTTC 4 cut(s) 316, 713, 838, 1043
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 922
Eco147I AGGCCT 1 cut(s) 1057
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 1225
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 922
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 922
FaeI CATG 9 cut(s) 163, 214, 391, 647, 661, 796, 988, 1235, 1276
FaqI GGGAC 4 cut(s) 385, 893, 1163, 1238
FatI CATG 9 cut(s) 159, 210, 387, 643, 657, 792, 984, 1231, 1272
FauI CCCGC 1 cut(s) 95
FbaI TGATCA 1 cut(s) 390
FblI GTMKAC 2 cut(s) 134, 840
Fnu4HI GCNGC 4 cut(s) 74, 243, 285, 1209
FokI GGATG 3 cut(s) 323, 914, 996
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 4 cut(s) 74, 243, 285, 1209
FspBI CTAG 3 cut(s) 605, 789, 1053
GlaI GCGC 2 cut(s) 118, 1126
GluI GCNGC 4 cut(s) 74, 243, 285, 1209
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 465, 1188
HaeIII GGCC 8 cut(s) 152, 192, 242, 275, 737, 1057, 1281, 1328
HapII CCGG 8 cut(s) 9, 35, 51, 114, 153, 170, 276, 1228
HgaI GACGC 3 cut(s) 187, 276, 476
HhaI GCGC 2 cut(s) 119, 1127
Hin1I GRCGYC 3 cut(s) 198, 287, 487
Hin1II CATG 9 cut(s) 163, 214, 391, 647, 661, 796, 988, 1235, 1276
Hin6I GCGC 2 cut(s) 117, 1125
HinP1I GCGC 2 cut(s) 117, 1125
HincII GTYRAC 3 cut(s) 135, 361, 681
HindII GTYRAC 3 cut(s) 135, 361, 681
HpaII CCGG 8 cut(s) 9, 35, 51, 114, 153, 170, 276, 1228
HphI GGTGA 3 cut(s) 428, 479, 1084
Hpy166II GTNNAC 7 cut(s) 68, 135, 361, 385, 681, 841, 1164
Hpy188I TCNGA 1 cut(s) 1100
Hpy188III TCNNGA 3 cut(s) 202, 414, 1273
Hpy8I GTNNAC 7 cut(s) 68, 135, 361, 385, 681, 841, 1164
Hpy99I CGWCG 6 cut(s) 17, 139, 142, 178, 203, 292
HpyAV CCTTC 5 cut(s) 175, 449, 748, 1056, 1105
HpyCH4III ACNGT 5 cut(s) 65, 715, 1076, 1183, 1316
HpyCH4IV ACGT 2 cut(s) 692, 1152
HpyCH4V TGCA 7 cut(s) 76, 227, 617, 760, 979, 1085, 1188
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 281, 667, 985, 1292
HpyF3I CTNAG 4 cut(s) 237, 260, 947, 1220
HpySE526I ACGT 2 cut(s) 692, 1152
Hsp92I GRCGYC 3 cut(s) 198, 287, 487
Hsp92II CATG 9 cut(s) 163, 214, 391, 647, 661, 796, 988, 1235, 1276
HspAI GCGC 2 cut(s) 117, 1125
KroI GCCGGC 2 cut(s) 169, 275
KroNI GCCGGC 2 cut(s) 171, 277
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 5 cut(s) 156, 213, 390, 685, 765
LguI GCTCTTC 1 cut(s) 838
LmnI GCTCC 3 cut(s) 431, 532, 1169
Lsp1109I GCAGC 2 cut(s) 60, 1195
LweI GCATC 5 cut(s) 301, 936, 972, 1152, 1309
MaeI CTAG 3 cut(s) 605, 789, 1053
MaeII ACGT 2 cut(s) 692, 1152
MaeIII GTNAC 2 cut(s) 53, 730
MalI GATC 5 cut(s) 158, 215, 392, 687, 767
MboI GATC 5 cut(s) 156, 213, 390, 685, 765
MboII GAAGA 6 cut(s) 333, 700, 825, 1030, 1108, 1192
MhlI GDGCHC 2 cut(s) 436, 833
MluCI AATT 2 cut(s) 636, 1297
MlyI GAGTC 4 cut(s) 35, 367, 875, 1095
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 8 cut(s) 82, 203, 232, 317, 878, 992, 1046, 1362
MroNI GCCGGC 2 cut(s) 169, 275
MroXI GAANNNNTTC 3 cut(s) 421, 553, 707
MseI TTAA 4 cut(s) 633, 780, 821, 1088
MslI CAYNNNNRTG 2 cut(s) 215, 753
MspA1I CMGCKG 2 cut(s) 346, 629
MspCI CTTAAG 1 cut(s) 779
MspI CCGG 8 cut(s) 9, 35, 51, 114, 153, 170, 276, 1228
MspR9I CCNGG 3 cut(s) 51, 154, 664
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 5 cut(s) 170, 281, 667, 985, 1292
NaeI GCCGGC 2 cut(s) 171, 277
NciI CCSGG 2 cut(s) 51, 154
NdeII GATC 5 cut(s) 156, 213, 390, 685, 765
NgoMIV GCCGGC 2 cut(s) 169, 275
NlaIII CATG 9 cut(s) 163, 214, 391, 647, 661, 796, 988, 1235, 1276
NlaIV GGNNCC 3 cut(s) 151, 866, 1226
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 730
PagI TCATGA 1 cut(s) 1272
PceI AGGCCT 1 cut(s) 1057
PciSI GCTCTTC 1 cut(s) 838
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PdiI GCCGGC 2 cut(s) 171, 277
PdmI GAANNNNTTC 3 cut(s) 421, 553, 707
PfeI GAWTC 6 cut(s) 38, 554, 621, 703, 796, 1020
PflFI GACNNNGTC 1 cut(s) 25
PkrI GCNGC 4 cut(s) 75, 244, 286, 1210
PleI GAGTC 4 cut(s) 34, 367, 875, 1095
PpsI GAGTC 4 cut(s) 34, 367, 875, 1095
PshBI ATTAAT 2 cut(s) 821, 1088
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 662
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 3 cut(s) 151, 866, 1226
PspPI GGNCC 4 cut(s) 150, 190, 273, 1225
PstI CTGCAG 1 cut(s) 619
PsyI GACNNNGTC 1 cut(s) 25
PvuII CAGCTG 2 cut(s) 346, 629
RseI CAYNNNNRTG 2 cut(s) 215, 753
SacI GAGCTC 1 cut(s) 436
SalI GTCGAC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 838
SaqAI TTAA 4 cut(s) 633, 780, 821, 1088
SatI GCNGC 4 cut(s) 74, 243, 285, 1209
Sau3AI GATC 5 cut(s) 156, 213, 390, 685, 765
Sau96I GGNCC 4 cut(s) 150, 190, 273, 1225
SchI GAGTC 4 cut(s) 35, 367, 875, 1095
ScrFI CCNGG 3 cut(s) 51, 154, 664
SduI GDGCHC 2 cut(s) 436, 833
SfaNI GCATC 5 cut(s) 301, 936, 972, 1152, 1309
SfcI CTRYAG 1 cut(s) 615
SgrAI CRCCGGYG 1 cut(s) 8
SinI GGWCC 1 cut(s) 1225
SmiMI CAYNNNNRTG 2 cut(s) 215, 753
SmlI CTYRAG 2 cut(s) 779, 1353
SmoI CTYRAG 2 cut(s) 779, 1353
Sse9I AATT 2 cut(s) 636, 1297
SseBI AGGCCT 1 cut(s) 1057
SsiI CCGC 6 cut(s) 102, 119, 243, 284, 811, 961
SspI AATATT 1 cut(s) 825
SspMI CTAG 3 cut(s) 605, 789, 1053
SstI GAGCTC 1 cut(s) 436
StuI AGGCCT 1 cut(s) 1057
StyD4I CCNGG 3 cut(s) 49, 152, 662
StyI CCWWGG 1 cut(s) 922
TaaI ACNGT 5 cut(s) 65, 715, 1076, 1183, 1316
TaiI ACGT 2 cut(s) 695, 1155
TaqI TCGA 4 cut(s) 134, 425, 884, 1029
TaqII GACCGA 1 cut(s) 38
TasI AATT 2 cut(s) 636, 1297
TauI GCSGC 2 cut(s) 245, 287
TfiI GAWTC 6 cut(s) 38, 554, 621, 703, 796, 1020
Tru1I TTAA 4 cut(s) 633, 780, 821, 1088
Tru9I TTAA 4 cut(s) 633, 780, 821, 1088
TscAI CASTG 5 cut(s) 75, 702, 955, 1020, 1321
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 2 cut(s) 73, 1208
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 7 cut(s) 194, 546, 730, 998, 1038, 1123, 1261
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 5 cut(s) 75, 702, 955, 1020, 1321
Tth111I GACNNNGTC 1 cut(s) 25
Vha464I CTTAAG 1 cut(s) 779
VpaK11BI GGWCC 1 cut(s) 1225
VspI ATTAAT 2 cut(s) 821, 1088
XapI RAATTY 2 cut(s) 636, 1297
XmiI GTMKAC 2 cut(s) 134, 840
XmnI GAANNNNTTC 3 cut(s) 421, 553, 707
XspI CTAG 3 cut(s) 605, 789, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.