MD00G1129700.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012340
Physical Location & Seq
Forward (+)
27825846 .. 27829177
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1129700.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGAAAATCTGAAAAAACTGAAAGAGCCAAAGAAAGAAGAGAAAAGAGACGCCAAGAAATCTCCCTCCTCAGAACCATTCCTTATTCCGATCACCAGCGGTGGTGGTCAGCAGAGACTGTTGCGGTGGTGACTGGGGGTAACAGGGGGATTGGGTTTGAGCTTTCGAAGCAGCTTGCGGCACATGGAGTTACTGTTATACTCACATCCAGAGACCACAGTGTGGGGCTTGAAGCAGCTAAGGTCTTCCAAGAAGGTGGTCTCAATGTGTTTTGCCATCAGCTCGATGTGCTAGACATGGCGTCCATCATTGAGTTTTGTGACTGGTTGAAAGAAAACTACGGTGGGTTGGATATTCTGATAAACAATGCAGCTGTTAATTTCAATCAGGGTTCTGATAATACTGTCGAGTATGCCTCTCAGGTTATTACTACAAATTATTATGGCACCAAAAACATGATTCAAGCAATGATCCCCTTGATGAAACCTTCAGCTGCTGGAGCTCGTATTGTTAATGTGAGCTCAAGATTGGGTAGAATAAATGGCAGGCGGAATAAAATTGATGATGTGATTTTGAGAGAGAAATTTAGCAATTTGGACAGAGTCACTGAGGAACTGATTGATCAAACAGTGTCCAGTTTTCTACAACAAGTACAAGATGGCACTTGGGAATCAGGTGGGTGGCCTAAAACGTTCACCGACTACTCAGTATCAAAACTTGCAGTCAACACTTACACCAGGCTGGTGGCGAAGATGCTCTCTGACAGACCAGAAGGCCAGAAAATTTATATCAACTGCTACTGCCCAGGTTGGGTGAAGACTGCTATGACAGGTTATGCTGGGAATATATCAGCCGAAGAAGGAGCTGACACTGCAGTTTGGCTTGCCTTGCTTCCAGACCTGGCAATAACGGGGAAGTTTTTCGCTGAAAGACGTGAGATAAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00790 ko00980 ko01100 ko05204 map00590 map00790 map00980 map01100 map05204
Pfam Domains
Protein Families
adh_short

Protein Analysis

317

Amino Acids

35.32

Weight (kDa)

6.92

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 41 - 180 1.7e-28 short chain dehydrogenase
KR PF08659 42 - 157 4.1e-07 KR domain
Epimerase PF01370 43 - 175 1.4e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 48 - 179 2.7e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51030
fragaria_vesca FvH4_5g09740 FvH4_5g09740 FvH4_5g09740
malus_domestica MD00G1129700.v1.1 MD14G1145700.v1.1
prunus_persica Prupe.5G140000_v2.0.a1
pyrus_communis pycom06g12030 pycom14g12070
rosa_chinensis RchiOBHm_Chr7g0188491
rosa_laevigata RLG00000004690
rosa_multiflora Rmu_sc0001576.1_g000006 Rmu_sc0016427.1_g000004
rosa_roxburghii Rroxscaffold_3G00266360
rosa_rugosa Rorug06G0490900
rosa_samantha Rh7AG095500 Rh7BG097200 Rh7DG097400
rosa_wichuraiana Rw7G008250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 223
AciI CCGC 4 cut(s) 100, 125, 179, 550
AclI AACGTT 1 cut(s) 692
AclWI GGATC 1 cut(s) 466
AcsI RAATTY 2 cut(s) 584, 783
AcuI CTGAAG 1 cut(s) 474
AcyI GRCGYC 2 cut(s) 52, 302
AdeI CACNNNGTG 1 cut(s) 223
AfaI GTAC 1 cut(s) 654
AfiI CCNNNNNNNGG 2 cut(s) 223, 811
AgsI TTSAA 4 cut(s) 233, 331, 385, 464
AhdI GACNNNNNGTC 1 cut(s) 301
AjiI CACGTC 1 cut(s) 935
AjnI CCWGG 3 cut(s) 737, 805, 900
Alw21I GWGCWC 2 cut(s) 505, 524
Alw26I GTCTC 4 cut(s) 43, 110, 207, 266
AlwI GGATC 1 cut(s) 466
AlwNI CAGNNNCTG 2 cut(s) 119, 497
AoxI GGCC 2 cut(s) 683, 775
ApeKI GCWGC 4 cut(s) 172, 236, 371, 494
ApoI RAATTY 2 cut(s) 584, 783
Asp700I GAANNNNTTC 1 cut(s) 920
AsuHPI GGTGA 4 cut(s) 86, 142, 688, 826
AsuII TTCGAA 1 cut(s) 167
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 2 cut(s) 505, 524
BbsI GAAGAC 2 cut(s) 238, 824
Bbv12I GWGCWC 2 cut(s) 505, 524
BbvI GCAGC 4 cut(s) 184, 248, 383, 481
BccI CCATC 3 cut(s) 285, 314, 653
BciT130I CCWGG 3 cut(s) 739, 807, 902
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 4 cut(s) 43, 110, 207, 266
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 873
BisI GCNGC 5 cut(s) 173, 180, 237, 372, 495
BlsI GCNGC 5 cut(s) 174, 181, 238, 373, 496
Bme1390I CCNGG 3 cut(s) 739, 807, 902
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgBI CACGTC 1 cut(s) 935
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 3 cut(s) 739, 807, 902
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 744
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 2 cut(s) 238, 824
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 508
BsaHI GRCGYC 2 cut(s) 52, 302
BsaI GGTCTC 2 cut(s) 207, 266
BsaJI CCNNGG 1 cut(s) 805
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 811
Bse1I ACTGG 3 cut(s) 139, 329, 636
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 3 cut(s) 739, 807, 902
BseDI CCNNGG 1 cut(s) 805
BseGI GGATG 1 cut(s) 206
BseLI CCNNNNNNNGG 2 cut(s) 223, 811
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 4 cut(s) 85, 434, 600, 720
BseNI ACTGG 3 cut(s) 139, 329, 636
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 4 cut(s) 184, 248, 383, 481
BseYI CCCAGC 1 cut(s) 839
BshFI GGCC 2 cut(s) 685, 777
BshNI GGYRCC 1 cut(s) 446
BsiHKAI GWGCWC 2 cut(s) 505, 524
BslI CCNNNNNNNGG 2 cut(s) 223, 811
BsmAI GTCTC 4 cut(s) 43, 110, 207, 266
BsmBI CGTCTC 1 cut(s) 43
BsnI GGCC 2 cut(s) 685, 777
Bso31I GGTCTC 2 cut(s) 207, 266
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 505, 524
Bsp143I GATC 3 cut(s) 91, 471, 622
BspACI CCGC 4 cut(s) 100, 125, 179, 550
BspANI GGCC 2 cut(s) 685, 777
BspCNI CTCAG 4 cut(s) 84, 433, 601, 719
BspLI GGNNCC 1 cut(s) 448
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 1 cut(s) 466
BspT104I TTCGAA 1 cut(s) 167
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 2 cut(s) 207, 266
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 3 cut(s) 139, 329, 636
BssECI CCNNGG 1 cut(s) 805
BssMI GATC 3 cut(s) 91, 471, 622
BssNI GRCGYC 2 cut(s) 52, 302
Bst2UI CCWGG 3 cut(s) 739, 807, 902
Bst4CI ACNGT 6 cut(s) 121, 196, 221, 344, 406, 631
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 2 cut(s) 52, 302
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 177, 548, 885
BstDEI CTNAG 5 cut(s) 71, 240, 420, 609, 706
BstF5I GGATG 1 cut(s) 206
BstKTI GATC 3 cut(s) 94, 474, 625
BstMAI GTCTC 4 cut(s) 43, 110, 207, 266
BstMBI GATC 3 cut(s) 91, 471, 622
BstMWI GCNNNNNNNGC 5 cut(s) 169, 289, 500, 872, 889
BstNI CCWGG 3 cut(s) 739, 807, 902
BstSCI CCNGG 3 cut(s) 737, 805, 900
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 4 cut(s) 184, 248, 383, 481
BstV2I GAAGAC 2 cut(s) 238, 824
BstXI CCANNNNNNTGG 2 cut(s) 257, 745
BsuRI GGCC 2 cut(s) 685, 777
BtrI CACGTC 1 cut(s) 935
BtsCI GGATG 1 cut(s) 206
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 4 cut(s) 226, 606, 636, 870
Cac8I GCNNGC 3 cut(s) 177, 548, 885
CaiI CAGNNNCTG 2 cut(s) 119, 497
CseI GACGC 2 cut(s) 60, 291
Csp6I GTAC 1 cut(s) 653
CviAII CATG 3 cut(s) 185, 298, 457
CviQI GTAC 1 cut(s) 653
DdeI CTNAG 5 cut(s) 71, 240, 420, 609, 706
DpnI GATC 3 cut(s) 93, 473, 624
DpnII GATC 3 cut(s) 91, 471, 622
DraIII CACNNNGTG 1 cut(s) 223
DriI GACNNNNNGTC 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 34
Eam1105I GACNNNNNGTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 1 cut(s) 565
Ecl136II GAGCTC 2 cut(s) 503, 522
Eco24I GRGCYC 2 cut(s) 505, 524
Eco31I GGTCTC 2 cut(s) 207, 266
Eco53kI GAGCTC 2 cut(s) 503, 522
Eco57I CTGAAG 1 cut(s) 474
EcoICRI GAGCTC 2 cut(s) 503, 522
EcoRII CCWGG 3 cut(s) 737, 805, 900
EcoT38I GRGCYC 2 cut(s) 505, 524
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 3 cut(s) 188, 301, 460
FatI CATG 3 cut(s) 184, 297, 456
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 5 cut(s) 173, 180, 237, 372, 495
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 2 cut(s) 505, 524
Fsp4HI GCNGC 5 cut(s) 173, 180, 237, 372, 495
FspBI CTAG 1 cut(s) 293
GluI GCNGC 5 cut(s) 173, 180, 237, 372, 495
GsaI CCCAGC 1 cut(s) 843
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 2 cut(s) 685, 777
HgaI GACGC 2 cut(s) 60, 291
Hin1I GRCGYC 2 cut(s) 52, 302
Hin1II CATG 3 cut(s) 188, 301, 460
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 3 cut(s) 460, 603, 671
HphI GGTGA 4 cut(s) 86, 142, 688, 826
Hpy166II GTNNAC 2 cut(s) 696, 727
Hpy188I TCNGA 6 cut(s) 13, 74, 91, 360, 397, 763
Hpy188III TCNNGA 3 cut(s) 210, 525, 896
Hpy8I GTNNAC 2 cut(s) 696, 727
HpyAV CCTTC 4 cut(s) 248, 498, 767, 854
HpyCH4III ACNGT 6 cut(s) 121, 196, 221, 344, 406, 631
HpyCH4IV ACGT 2 cut(s) 692, 934
HpyCH4V TGCA 3 cut(s) 371, 722, 875
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 289, 500, 872, 889
HpyF3I CTNAG 5 cut(s) 71, 240, 420, 609, 706
HpySE526I ACGT 2 cut(s) 692, 934
Hsp92I GRCGYC 2 cut(s) 52, 302
Hsp92II CATG 3 cut(s) 188, 301, 460
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 3 cut(s) 91, 471, 622
LmnI GCTCC 2 cut(s) 500, 863
Lsp1109I GCAGC 4 cut(s) 184, 248, 383, 481
LweI GCATC 1 cut(s) 744
MaeI CTAG 1 cut(s) 293
MaeII ACGT 2 cut(s) 692, 934
MaeIII GTNAC 5 cut(s) 130, 140, 190, 320, 604
MalI GATC 3 cut(s) 93, 473, 624
MboI GATC 3 cut(s) 91, 471, 622
MboII GAAGA 5 cut(s) 51, 238, 763, 829, 869
MhlI GDGCHC 2 cut(s) 505, 524
MluCI AATT 6 cut(s) 379, 436, 558, 584, 592, 783
MlyI GAGTC 1 cut(s) 612
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 4 cut(s) 77, 80, 427, 604
MroXI GAANNNNTTC 1 cut(s) 920
MseI TTAA 2 cut(s) 378, 513
MspA1I CMGCKG 3 cut(s) 100, 374, 494
MspR9I CCNGG 3 cut(s) 739, 807, 902
MvaI CCWGG 3 cut(s) 739, 807, 902
MwoI GCNNNNNNNGC 5 cut(s) 169, 289, 500, 872, 889
NdeII GATC 3 cut(s) 91, 471, 622
NlaIII CATG 3 cut(s) 188, 301, 460
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 3 cut(s) 130, 320, 604
NspV TTCGAA 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 920
PfeI GAWTC 2 cut(s) 460, 671
PflFI GACNNNGTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 5 cut(s) 174, 181, 238, 373, 496
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
Psp124BI GAGCTC 2 cut(s) 505, 524
Psp1406I AACGTT 1 cut(s) 692
Psp6I CCWGG 3 cut(s) 737, 805, 900
PspFI CCCAGC 1 cut(s) 839
PspGI CCWGG 3 cut(s) 737, 805, 900
PspN4I GGNNCC 1 cut(s) 448
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 2 cut(s) 119, 497
PsyI GACNNNGTC 1 cut(s) 602
PvuII CAGCTG 2 cut(s) 374, 494
RsaI GTAC 1 cut(s) 654
RsaNI GTAC 1 cut(s) 653
SacI GAGCTC 2 cut(s) 505, 524
SaqAI TTAA 2 cut(s) 378, 513
SatI GCNGC 5 cut(s) 173, 180, 237, 372, 495
Sau3AI GATC 3 cut(s) 91, 471, 622
SchI GAGTC 1 cut(s) 612
ScrFI CCNGG 3 cut(s) 739, 807, 902
SduI GDGCHC 2 cut(s) 505, 524
SfaNI GCATC 1 cut(s) 744
SfcI CTRYAG 1 cut(s) 873
SfuI TTCGAA 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
Sse9I AATT 6 cut(s) 379, 436, 558, 584, 592, 783
SsiI CCGC 4 cut(s) 100, 125, 179, 550
SspMI CTAG 1 cut(s) 293
SstI GAGCTC 2 cut(s) 505, 524
StyD4I CCNGG 3 cut(s) 737, 805, 900
TaaI ACNGT 6 cut(s) 121, 196, 221, 344, 406, 631
TaiI ACGT 2 cut(s) 695, 937
TaqI TCGA 3 cut(s) 167, 285, 408
TasI AATT 6 cut(s) 379, 436, 558, 584, 592, 783
TatI WGTACW 1 cut(s) 652
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 2 cut(s) 460, 671
Tru1I TTAA 2 cut(s) 378, 513
Tru9I TTAA 2 cut(s) 378, 513
TscAI CASTG 4 cut(s) 226, 613, 636, 877
TseFI GTSAC 3 cut(s) 130, 320, 604
TseI GCWGC 4 cut(s) 172, 236, 371, 494
Tsp45I GTSAC 3 cut(s) 130, 320, 604
TspDTI ATGAA 1 cut(s) 497
TspRI CASTG 4 cut(s) 226, 613, 636, 877
Tth111I GACNNNGTC 1 cut(s) 602
Van91I CCANNNNNTGG 1 cut(s) 223
XapI RAATTY 2 cut(s) 584, 783
XmnI GAANNNNTTC 1 cut(s) 920
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.