MD14G1145700.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012340
Physical Location & Seq
Forward (+)
23862794 .. 23865507
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1145700.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGAAAAATCTGAAAGAGCCAAAGAAAGAAAAGAAAAGAGACTTCAAGAAATCTCCCTCCTCAGAACCATTCCTTATTCCGATCACCAGAGGTGGTGGTCAGCAGAGACCGTTGCGGTGGTGACTGGGGGTAACAGGGGGATTGGGTTTGAGATTTCGAAGCAGCTTGCGGCACAAGGGGTAACTGTTATACTCACATCCAGAGATCACAGTGTGGGGCTTGAAGCGGCTAAGGTCTTCCAAGAAGGTGGTTTCAATGTGTTTTGTCACCAGCTCGATGTGTTAGACACCGCGTCCATCACCGAGTTTTGTGACTGGTTGAAACAAAACTATGGTGGGTTGGATATTCTGATAAATAATGCAGCTGTTAATTTCAATCAGGGTTTTGATAATACTGTCGAGTATGCCTCTCAGGTTATTACTACAAATTATTATGGCACCAAAAATATGATTCAAGCAATGATCCCCTTGATGAAACCTTCAGCTGCTGGAGCTCGTATTGTTAATGTGAGCTCAAGATTGGGTAGAATAAATGGCAAGCGGAGTAAAATTGAAGATGTGTGTTTGAGAGAGAAATTTAGCAATTTGGACACAATCACTGAGGAACTGATTGATCAAACAGTTTCCAGTTTTCTACAACAAGTACAAGATGGCACTTGGGAATCCGGGGGGTGGCCTAAAACGTTCACTGACTACTCGGTATCAAAACTTGCAGTCAACACTTACACCAGGCTGATGGCAAAGATGCTATCCGACAGACCAGAAGGCCAGAAAATTTATATCAACTGTTACTGCCCAGGTTGGGTGAAGACTGCTATGACAGGTTATGCTGGGAATATTTCAGTTGAAGCAGGGGCTGACACTGCAGTTTGGCTTGCCTTGCTTCCAGACCTGGCAGTAACGGGGAAGTTTTTCGCTGAAAGACGTGAGATAAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00790 ko00980 ko01100 ko05204 map00590 map00790 map00980 map01100 map05204
Pfam Domains
Protein Families
adh_short

Protein Analysis

314

Amino Acids

34.9

Weight (kDa)

6.36

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 38 - 178 1.7e-29 short chain dehydrogenase
KR PF08659 39 - 154 4e-08 KR domain
Epimerase PF01370 40 - 183 1e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 45 - 177 7.8e-22 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51030
fragaria_vesca FvH4_5g09740 FvH4_5g09740 FvH4_5g09740
malus_domestica MD00G1129700.v1.1 MD14G1145700.v1.1
prunus_persica Prupe.5G140000_v2.0.a1
pyrus_communis pycom06g12030 pycom14g12070
rosa_chinensis RchiOBHm_Chr7g0188491
rosa_laevigata RLG00000004690
rosa_multiflora Rmu_sc0001576.1_g000006 Rmu_sc0016427.1_g000004
rosa_roxburghii Rroxscaffold_3G00266360
rosa_rugosa Rorug06G0490900
rosa_samantha Rh7AG095500 Rh7BG097200 Rh7DG097400
rosa_wichuraiana Rw7G008250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 437
AccII CGCG 1 cut(s) 293
AciI CCGC 5 cut(s) 116, 170, 227, 291, 541
AclI AACGTT 1 cut(s) 683
AclWI GGATC 1 cut(s) 457
AcsI RAATTY 2 cut(s) 575, 774
AcuI CTGAAG 1 cut(s) 465
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 1 cut(s) 645
AfiI CCNNNNNNNGG 2 cut(s) 672, 802
AgsI TTSAA 8 cut(s) 47, 224, 256, 322, 376, 455, 554, 848
AhdI GACNNNNNGTC 1 cut(s) 292
AjiI CACGTC 1 cut(s) 926
AjnI CCWGG 3 cut(s) 728, 796, 891
AluBI AGCT 7 cut(s) 166, 274, 365, 485, 494, 513, 936
AluI AGCT 7 cut(s) 166, 274, 365, 485, 494, 513, 936
Alw21I GWGCWC 2 cut(s) 496, 515
Alw26I GTCTC 2 cut(s) 34, 101
AlwI GGATC 1 cut(s) 457
AlwNI CAGNNNCTG 2 cut(s) 488, 857
AoxI GGCC 2 cut(s) 674, 766
ApeKI GCWGC 3 cut(s) 163, 362, 485
ApoI RAATTY 2 cut(s) 575, 774
Asp700I GAANNNNTTC 1 cut(s) 911
AsuC2I CCSGG 1 cut(s) 667
AsuHPI GGTGA 5 cut(s) 77, 133, 260, 292, 817
AsuII TTCGAA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 2 cut(s) 496, 515
BbsI GAAGAC 2 cut(s) 229, 815
Bbv12I GWGCWC 2 cut(s) 496, 515
BbvI GCAGC 3 cut(s) 175, 374, 472
BccI CCATC 3 cut(s) 305, 644, 730
BciT130I CCWGG 3 cut(s) 730, 798, 893
BclI TGATCA 1 cut(s) 613
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 2 cut(s) 34, 101
BfmI CTRYAG 1 cut(s) 864
BisI GCNGC 5 cut(s) 164, 171, 228, 363, 486
BlsI GCNGC 5 cut(s) 165, 172, 229, 364, 487
Bme1390I CCNGG 4 cut(s) 667, 730, 798, 893
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgBI CACGTC 1 cut(s) 926
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 4 cut(s) 667, 730, 798, 893
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 735
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 2 cut(s) 229, 815
BplI GAGNNNNNCTC 2 cut(s) 392, 424
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 231
Bpu14I TTCGAA 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 499
BpuMI CCSGG 1 cut(s) 667
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 666, 796
Bsc4I CCNNNNNNNGG 2 cut(s) 672, 802
Bse1I ACTGG 3 cut(s) 130, 320, 627
Bse3DI GCAATG 1 cut(s) 465
BseBI CCWGG 3 cut(s) 730, 798, 893
BseDI CCNNGG 2 cut(s) 666, 796
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 2 cut(s) 672, 802
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 3 cut(s) 76, 425, 591
BseNI ACTGG 3 cut(s) 130, 320, 627
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 3 cut(s) 175, 374, 472
BseYI CCCAGC 1 cut(s) 830
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 2 cut(s) 676, 768
BshNI GGYRCC 1 cut(s) 437
BsiHKAI GWGCWC 2 cut(s) 496, 515
BsiSI CCGG 1 cut(s) 666
BslI CCNNNNNNNGG 2 cut(s) 672, 802
BsmAI GTCTC 2 cut(s) 34, 101
BsnI GGCC 2 cut(s) 676, 768
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 496, 515
Bsp143I GATC 4 cut(s) 82, 205, 462, 613
BspACI CCGC 5 cut(s) 116, 170, 227, 291, 541
BspANI GGCC 2 cut(s) 676, 768
BspCNI CTCAG 3 cut(s) 75, 424, 592
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 1 cut(s) 439
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 1 cut(s) 457
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 465
BsrI ACTGG 3 cut(s) 130, 320, 627
BssECI CCNNGG 2 cut(s) 666, 796
BssMI GATC 4 cut(s) 82, 205, 462, 613
Bst2UI CCWGG 3 cut(s) 730, 798, 893
Bst4CI ACNGT 6 cut(s) 112, 187, 212, 397, 622, 788
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 168, 539, 876
BstDEI CTNAG 4 cut(s) 62, 231, 411, 600
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 293
BstKTI GATC 4 cut(s) 85, 208, 465, 616
BstMAI GTCTC 2 cut(s) 34, 101
BstMBI GATC 4 cut(s) 82, 205, 462, 613
BstMWI GCNNNNNNNGC 3 cut(s) 491, 863, 880
BstNI CCWGG 3 cut(s) 730, 798, 893
BstSCI CCNGG 4 cut(s) 665, 728, 796, 891
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 3 cut(s) 175, 374, 472
BstV2I GAAGAC 2 cut(s) 229, 815
BstXI CCANNNNNNTGG 2 cut(s) 248, 736
BsuRI GGCC 2 cut(s) 676, 768
BtrI CACGTC 1 cut(s) 926
BtsCI GGATG 1 cut(s) 197
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 4 cut(s) 217, 597, 687, 861
Cac8I GCNNGC 3 cut(s) 168, 539, 876
CaiI CAGNNNCTG 2 cut(s) 488, 857
CseI GACGC 1 cut(s) 282
Csp6I GTAC 1 cut(s) 644
CviQI GTAC 1 cut(s) 644
DdeI CTNAG 4 cut(s) 62, 231, 411, 600
DpnI GATC 4 cut(s) 84, 207, 464, 615
DpnII GATC 4 cut(s) 82, 205, 462, 613
DraIII CACNNNGTG 1 cut(s) 214
DriI GACNNNNNGTC 1 cut(s) 292
Eam1105I GACNNNNNGTC 1 cut(s) 292
Ecl136II GAGCTC 2 cut(s) 494, 513
Eco24I GRGCYC 2 cut(s) 496, 515
Eco31I GGTCTC 1 cut(s) 101
Eco53kI GAGCTC 2 cut(s) 494, 513
Eco57I CTGAAG 1 cut(s) 465
EcoICRI GAGCTC 2 cut(s) 494, 513
EcoRII CCWGG 3 cut(s) 728, 796, 891
EcoT38I GRGCYC 2 cut(s) 496, 515
FaiI YATR 8 cut(s) 191, 333, 405, 435, 449, 780, 818, 828
FbaI TGATCA 1 cut(s) 613
Fnu4HI GCNGC 5 cut(s) 164, 171, 228, 363, 486
FokI GGATG 1 cut(s) 184
FriOI GRGCYC 2 cut(s) 496, 515
Fsp4HI GCNGC 5 cut(s) 164, 171, 228, 363, 486
GluI GCNGC 5 cut(s) 164, 171, 228, 363, 486
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 2 cut(s) 676, 768
HapII CCGG 1 cut(s) 666
HgaI GACGC 1 cut(s) 282
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 934
HinfI GANTC 2 cut(s) 451, 662
HpaII CCGG 1 cut(s) 666
HphI GGTGA 5 cut(s) 77, 133, 260, 292, 817
Hpy166II GTNNAC 2 cut(s) 687, 718
Hpy188I TCNGA 5 cut(s) 13, 65, 82, 351, 754
Hpy188III TCNNGA 4 cut(s) 47, 201, 516, 887
Hpy8I GTNNAC 2 cut(s) 687, 718
HpyAV CCTTC 3 cut(s) 239, 489, 758
HpyCH4III ACNGT 6 cut(s) 112, 187, 212, 397, 622, 788
HpyCH4IV ACGT 2 cut(s) 683, 925
HpyCH4V TGCA 3 cut(s) 362, 713, 866
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 863, 880
HpyF3I CTNAG 4 cut(s) 62, 231, 411, 600
HpySE526I ACGT 2 cut(s) 683, 925
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 4 cut(s) 82, 205, 462, 613
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 3 cut(s) 175, 374, 472
LweI GCATC 1 cut(s) 735
MaeII ACGT 2 cut(s) 683, 925
MaeIII GTNAC 7 cut(s) 121, 131, 181, 266, 311, 788, 898
MalI GATC 4 cut(s) 84, 207, 464, 615
MboI GATC 4 cut(s) 82, 205, 462, 613
MboII GAAGA 3 cut(s) 229, 566, 820
MhlI GDGCHC 2 cut(s) 496, 515
MluCI AATT 6 cut(s) 370, 427, 549, 575, 583, 774
MmeI TCCRAC 2 cut(s) 321, 777
MnlI CCTC 5 cut(s) 68, 71, 84, 418, 595
MroXI GAANNNNTTC 1 cut(s) 911
MseI TTAA 2 cut(s) 369, 504
MspA1I CMGCKG 2 cut(s) 365, 485
MspI CCGG 1 cut(s) 666
MspR9I CCNGG 4 cut(s) 667, 730, 798, 893
MvaI CCWGG 3 cut(s) 730, 798, 893
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 491, 863, 880
NciI CCSGG 1 cut(s) 667
NdeII GATC 4 cut(s) 82, 205, 462, 613
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 3 cut(s) 121, 266, 311
NspV TTCGAA 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 2 cut(s) 451, 662
PkrI GCNGC 5 cut(s) 165, 172, 229, 364, 487
Psp124BI GAGCTC 2 cut(s) 496, 515
Psp1406I AACGTT 1 cut(s) 683
Psp6I CCWGG 3 cut(s) 728, 796, 891
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 3 cut(s) 728, 796, 891
PspN4I GGNNCC 1 cut(s) 439
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 2 cut(s) 488, 857
PvuII CAGCTG 2 cut(s) 365, 485
RsaI GTAC 1 cut(s) 645
RsaNI GTAC 1 cut(s) 644
SacI GAGCTC 2 cut(s) 496, 515
SaqAI TTAA 2 cut(s) 369, 504
SatI GCNGC 5 cut(s) 164, 171, 228, 363, 486
Sau3AI GATC 4 cut(s) 82, 205, 462, 613
ScrFI CCNGG 4 cut(s) 667, 730, 798, 893
SduI GDGCHC 2 cut(s) 496, 515
SfaNI GCATC 1 cut(s) 735
SfcI CTRYAG 1 cut(s) 864
SfuI TTCGAA 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 514
SmoI CTYRAG 1 cut(s) 514
Sse9I AATT 6 cut(s) 370, 427, 549, 575, 583, 774
SsiI CCGC 5 cut(s) 116, 170, 227, 291, 541
SspI AATATT 1 cut(s) 838
SstI GAGCTC 2 cut(s) 496, 515
StyD4I CCNGG 4 cut(s) 665, 728, 796, 891
TaaI ACNGT 6 cut(s) 112, 187, 212, 397, 622, 788
TaiI ACGT 2 cut(s) 686, 928
TaqI TCGA 3 cut(s) 158, 276, 399
TasI AATT 6 cut(s) 370, 427, 549, 575, 583, 774
TatI WGTACW 1 cut(s) 643
TauI GCSGC 2 cut(s) 173, 230
TfiI GAWTC 2 cut(s) 451, 662
Tru1I TTAA 2 cut(s) 369, 504
Tru9I TTAA 2 cut(s) 369, 504
TscAI CASTG 4 cut(s) 217, 604, 694, 868
TseFI GTSAC 3 cut(s) 121, 266, 311
TseI GCWGC 3 cut(s) 163, 362, 485
Tsp45I GTSAC 3 cut(s) 121, 266, 311
TspDTI ATGAA 1 cut(s) 488
TspRI CASTG 4 cut(s) 217, 604, 694, 868
XapI RAATTY 2 cut(s) 575, 774
XmnI GAANNNNTTC 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.