Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0011530

Physical Location & Seq

Genomic Coordinates Forward (+)

31227045 .. 31229422

Gene Structure 2378 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1143500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGACTCAACGTGGGTGAACACTTCTCTCGGCCTTAATCTTGTTCCGTTTTGCCGTGCCAATGATAGTCGAGCTCCTGCCACTATGCAACAAGAGTTTGAAGGGGGTTATGCTCAACTCCAAGGCAATGCATTGGTGAAAGAAGAGGCAGCTAGTCATCATGATGTGTTGGCTGAAGAGTTGAATAGGGTAAGTGTGGAGAACAAGAAGCTGACTGAGATGCTAACTGCCCTCTGCGAGAATTACAACACTTTGCAATCCCAAGTGGGGGAATTGATGACTACCCAGCAGCAGCAAAACAAAACATCTGATATTTATAGGAAGCGAAAGGCAGACAGTGAAGATTACAACAACGTGATTGGATTAATTAGTCCCGGGACTAATACTGACACCAGTTCCATCAGTGATGATAAAGAGTATTCATGCAAGAGGCTAAAGGAGAACACCAACTTAAAGATTTCCAGGGTTTATGTTCACACCGATGCATCCGATACAGGCCTCATTGTGAATGATGGATACCAATGGAGGAAATATGGTCAGAAGGTCACAAGAGATAACCCATCTCCTAGGGCTTACTACAAGTGCTCCTTTGCCCCAAGTTGCCCTGTTAAAAAGAAGGTGCAAAAAAGTGCTGAAAATCCATGCCTACTGGTGGCTACATATGAAGGAGAACACAACCACATGTATCCTGAGAAGCGAGCTGAAGTTACACTAATAGCCCCGGTATCTCCAAAACAGAACCAGCAGCTTATTACTAGTCCAATTTCCGTTTTACCATCCGTGGCAAAGATATCTCCGGCTCCAACTTTTTTAAGTGACGATAATCGAATAAGTCCCAATAGTGCCATGTGCCCCCCAGAAACTCAAGCTCCGACGTTCCAACAGTTTTTGGTACAACAAATGGCTTCTTCCCTGACCAAAGATCCCAATTTCACATCTGCTCTTGCTGCTGCCATTTCAGGAAGAATTTCAGGCCACACTATGGCCCGAAATGGTGAAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0002237 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0009890 GO:0009892 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046677 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

335

Amino Acids

36.98

Weight (kDa)

7.0

Isoelectric Point (pI)

44.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	170 - 227	6.6e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011530)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g41060 FvH4_2g41060 FvH4_2g41060
malus_domestica	MD00G1143500.v1.1 MD15G1039500.v1.1
prunus_persica	Prupe.1G393000_v2.0.a1
pyrus_communis	pycom08g04760 pycom15g03690
rosa_chinensis	RchiOBHm_Chr6g0299501
rosa_laevigata	RLG00000011426
rosa_multiflora	Rmu_sc0001184.1_g000004
rosa_roxburghii	Rroxscaffold_7G00168630
rosa_rugosa	Rorug06G0289200 Rorug06G0289200 Rorug06G0289300
rosa_samantha	Rh6AG400500 Rh6BG409200 Rh6CG414700 Rh6DG401600
rosa_wichuraiana	Rw6G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	982
AclWI	GGATC	1 cut(s)	917
AcsI	RAATTY	1 cut(s)	966
AcuI	CTGAAG	2 cut(s)	195, 723
AfaI	GTAC	1 cut(s)	894
AfiI	CCNNNNNNNGG	4 cut(s)	267, 268, 652, 982
AflIII	ACRYGT	1 cut(s)	681
AgsI	TTSAA	2 cut(s)	101, 184
AhlI	ACTAGT	1 cut(s)	755
AjnI	CCWGG	1 cut(s)	461
AluBI	AGCT	6 cut(s)	74, 152, 211, 701, 748, 869
AluI	AGCT	6 cut(s)	74, 152, 211, 701, 748, 869
Alw21I	GWGCWC	2 cut(s)	76, 587
AlwI	GGATC	1 cut(s)	917
Ama87I	CYCGRG	1 cut(s)	374
AoxI	GGCC	4 cut(s)	31, 496, 973, 984
ApeKI	GCWGC	6 cut(s)	149, 289, 292, 745, 947, 950
ApoI	RAATTY	1 cut(s)	966
AseI	ATTAAT	1 cut(s)	365
Asp700I	GAANNNNTTC	1 cut(s)	967
AspA2I	CCTAGG	1 cut(s)	566
AspS9I	GGNCC	1 cut(s)	985
AsuC2I	CCSGG	3 cut(s)	375, 376, 722
AsuHPI	GGTGA	2 cut(s)	28, 148
AvaI	CYCGRG	1 cut(s)	374
AvrII	CCTAGG	1 cut(s)	566
BaeGI	GKGCMC	1 cut(s)	854
BanII	GRGCYC	1 cut(s)	76
Bbv12I	GWGCWC	2 cut(s)	76, 587
BbvI	GCAGC	6 cut(s)	161, 301, 304, 757, 934, 937
BccI	CCATC	4 cut(s)	407, 506, 568, 784
BceAI	ACGGC	1 cut(s)	39
BciT130I	CCWGG	1 cut(s)	463
BciVI	GTATCC	2 cut(s)	509, 696
BcnI	CCSGG	3 cut(s)	375, 376, 722
BcuI	ACTAGT	1 cut(s)	755
BfaI	CTAG	3 cut(s)	153, 567, 756
BfuI	GTATCC	2 cut(s)	509, 696
BisI	GCNGC	6 cut(s)	150, 290, 293, 746, 948, 951
BlnI	CCTAGG	1 cut(s)	566
BlsI	GCNGC	6 cut(s)	151, 291, 294, 747, 949, 952
Bme1390I	CCNGG	4 cut(s)	375, 376, 463, 722
BmeT110I	CYCGRG	1 cut(s)	374
BmgT120I	GGNCC	1 cut(s)	985
BmiI	GGNNCC	1 cut(s)	801
BmrFI	CCNGG	4 cut(s)	375, 376, 463, 722
BmsI	GCATC	3 cut(s)	210, 472, 494
BpuEI	CTTGAG	1 cut(s)	849
BpuMI	CCSGG	3 cut(s)	375, 376, 722
BsaJI	CCNNGG	6 cut(s)	121, 374, 462, 566, 720, 780
BsaXI	ACNNNNNCTCC	4 cut(s)	569, 599, 853, 883
Bsc4I	CCNNNNNNNGG	4 cut(s)	267, 268, 652, 982
Bse1I	ACTGG	2 cut(s)	393, 654
Bse3DI	GCAATG	1 cut(s)	133
BseBI	CCWGG	1 cut(s)	463
BseDI	CCNNGG	6 cut(s)	121, 374, 462, 566, 720, 780
BseGI	GGATG	2 cut(s)	485, 776
BseLI	CCNNNNNNNGG	4 cut(s)	267, 268, 652, 982
BseMI	GCAATG	1 cut(s)	133
BseMII	CTCAG	2 cut(s)	207, 681
BseNI	ACTGG	2 cut(s)	393, 654
BseSI	GKGCMC	1 cut(s)	854
BseXI	GCAGC	6 cut(s)	161, 301, 304, 757, 934, 937
BseYI	CCCAGC	1 cut(s)	285
BshFI	GGCC	4 cut(s)	33, 498, 975, 986
BsiHKAI	GWGCWC	2 cut(s)	76, 587
BsiHKCI	CYCGRG	1 cut(s)	374
BsiSI	CCGG	3 cut(s)	375, 722, 797
BslFI	GGGAC	3 cut(s)	357, 391, 819
BslI	CCNNNNNNNGG	4 cut(s)	267, 268, 652, 982
BsmFI	GGGAC	3 cut(s)	357, 391, 819
BsnI	GGCC	4 cut(s)	33, 498, 975, 986
BsoBI	CYCGRG	1 cut(s)	374
Bsp1286I	GDGCHC	3 cut(s)	76, 587, 854
Bsp143I	GATC	1 cut(s)	922
BspANI	GGCC	4 cut(s)	33, 498, 975, 986
BspCNI	CTCAG	2 cut(s)	208, 682
BspHI	TCATGA	1 cut(s)	160
BspLI	GGNNCC	1 cut(s)	801
BspPI	GGATC	1 cut(s)	917
BsrDI	GCAATG	1 cut(s)	133
BsrI	ACTGG	2 cut(s)	393, 654
BssECI	CCNNGG	6 cut(s)	121, 374, 462, 566, 720, 780
BssMI	GATC	1 cut(s)	922
BssT1I	CCWWGG	2 cut(s)	121, 566
Bst2UI	CCWGG	1 cut(s)	463
Bst4CI	ACNGT	2 cut(s)	338, 885
Bst6I	CTCTTC	2 cut(s)	138, 171
BstC8I	GCNNGC	1 cut(s)	699
BstDEI	CTNAG	2 cut(s)	216, 690
BstDSI	CCRYGG	1 cut(s)	780
BstF5I	GGATG	2 cut(s)	485, 776
BstKTI	GATC	1 cut(s)	925
BstMBI	GATC	1 cut(s)	922
BstMWI	GCNNNNNNNGC	1 cut(s)	947
BstNI	CCWGG	1 cut(s)	463
BstNSI	RCATGY	1 cut(s)	685
BstSCI	CCNGG	4 cut(s)	373, 374, 461, 720
BstSLI	GKGCMC	1 cut(s)	854
BstV1I	GCAGC	6 cut(s)	161, 301, 304, 757, 934, 937
BstX2I	RGATCY	1 cut(s)	922
BstYI	RGATCY	1 cut(s)	922
BsuI	GTATCC	2 cut(s)	509, 696
BsuRI	GGCC	4 cut(s)	33, 498, 975, 986
BtgI	CCRYGG	1 cut(s)	780
BtsCI	GGATG	2 cut(s)	485, 776
BtsIMutI	CAGTG	2 cut(s)	343, 409
Cac8I	GCNNGC	1 cut(s)	699
CciI	TCATGA	1 cut(s)	160
Cfr13I	GGNCC	1 cut(s)	985
Cfr9I	CCCGGG	1 cut(s)	374
Csp6I	GTAC	1 cut(s)	893
CviAII	CATG	5 cut(s)	161, 423, 642, 682, 847
CviQI	GTAC	1 cut(s)	893
DdeI	CTNAG	2 cut(s)	216, 690
DpnI	GATC	1 cut(s)	924
DpnII	GATC	1 cut(s)	922
Eam1104I	CTCTTC	2 cut(s)	138, 171
EarI	CTCTTC	2 cut(s)	138, 171
Ecl136II	GAGCTC	1 cut(s)	74
Eco130I	CCWWGG	2 cut(s)	121, 566
Eco147I	AGGCCT	1 cut(s)	498
Eco24I	GRGCYC	1 cut(s)	76
Eco32I	GATATC	1 cut(s)	792
Eco53kI	GAGCTC	1 cut(s)	74
Eco57I	CTGAAG	2 cut(s)	195, 723
Eco88I	CYCGRG	1 cut(s)	374
EcoICRI	GAGCTC	1 cut(s)	74
EcoRII	CCWGG	1 cut(s)	461
EcoRV	GATATC	1 cut(s)	792
EcoT14I	CCWWGG	2 cut(s)	121, 566
EcoT22I	ATGCAT	2 cut(s)	133, 487
EcoT38I	GRGCYC	1 cut(s)	76
ErhI	CCWWGG	2 cut(s)	121, 566
FaeI	CATG	5 cut(s)	164, 426, 645, 685, 850
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	3 cut(s)	357, 391, 819
FatI	CATG	5 cut(s)	160, 422, 641, 681, 846
FauNDI	CATATG	1 cut(s)	661
Fnu4HI	GCNGC	6 cut(s)	150, 290, 293, 746, 948, 951
FokI	GGATG	2 cut(s)	472, 763
FriOI	GRGCYC	1 cut(s)	76
Fsp4HI	GCNGC	6 cut(s)	150, 290, 293, 746, 948, 951
FspBI	CTAG	3 cut(s)	153, 567, 756
GluI	GCNGC	6 cut(s)	150, 290, 293, 746, 948, 951
GsaI	CCCAGC	1 cut(s)	289
HaeIII	GGCC	4 cut(s)	33, 498, 975, 986
HapII	CCGG	3 cut(s)	375, 722, 797
Hin1II	CATG	5 cut(s)	164, 426, 645, 685, 850
HinfI	GANTC	1 cut(s)	5
HpaII	CCGG	3 cut(s)	375, 722, 797
HphI	GGTGA	2 cut(s)	28, 148
Hpy166II	GTNNAC	2 cut(s)	19, 475
Hpy188I	TCNGA	4 cut(s)	310, 490, 540, 873
Hpy188III	TCNNGA	3 cut(s)	161, 689, 960
Hpy8I	GTNNAC	2 cut(s)	19, 475
Hpy99I	CGWCG	1 cut(s)	877
HpyAV	CCTTC	4 cut(s)	95, 535, 610, 659
HpyCH4III	ACNGT	2 cut(s)	338, 885
HpyCH4IV	ACGT	3 cut(s)	11, 354, 875
HpyCH4V	TGCA	6 cut(s)	88, 131, 256, 426, 485, 622
HpyF10VI	GCNNNNNNNGC	1 cut(s)	947
HpyF3I	CTNAG	2 cut(s)	216, 690
HpySE526I	ACGT	3 cut(s)	11, 354, 875
Hsp92II	CATG	5 cut(s)	164, 426, 645, 685, 850
Kzo9I	GATC	1 cut(s)	922
LmnI	GCTCC	4 cut(s)	79, 590, 805, 874
Lsp1109I	GCAGC	6 cut(s)	161, 301, 304, 757, 934, 937
LweI	GCATC	3 cut(s)	210, 472, 494
MaeI	CTAG	3 cut(s)	153, 567, 756
MaeII	ACGT	3 cut(s)	11, 354, 875
MaeIII	GTNAC	3 cut(s)	544, 706, 815
MalI	GATC	1 cut(s)	924
MboI	GATC	1 cut(s)	922
MboII	GAAGA	5 cut(s)	155, 188, 353, 900, 975
MflI	RGATCY	1 cut(s)	922
MhlI	GDGCHC	3 cut(s)	76, 587, 854
MluCI	AATT	6 cut(s)	241, 272, 366, 762, 928, 966
MmeI	TCCRAC	3 cut(s)	827, 896, 904
MnlI	CCTC	5 cut(s)	139, 242, 423, 509, 519
Mph1103I	ATGCAT	2 cut(s)	133, 487
MroXI	GAANNNNTTC	1 cut(s)	967
MseI	TTAA	5 cut(s)	36, 365, 452, 609, 812
MslI	CAYNNNNRTG	2 cut(s)	162, 480
MspI	CCGG	3 cut(s)	375, 722, 797
MspR9I	CCNGG	4 cut(s)	375, 376, 463, 722
MvaI	CCWGG	1 cut(s)	463
MwoI	GCNNNNNNNGC	1 cut(s)	947
NciI	CCSGG	3 cut(s)	375, 376, 722
NdeI	CATATG	1 cut(s)	661
NdeII	GATC	1 cut(s)	922
NlaIII	CATG	5 cut(s)	164, 426, 645, 685, 850
NlaIV	GGNNCC	1 cut(s)	801
NmeAIII	GCCGAG	1 cut(s)	9
NmuCI	GTSAC	2 cut(s)	544, 815
NsiI	ATGCAT	2 cut(s)	133, 487
NspI	RCATGY	1 cut(s)	685
PagI	TCATGA	1 cut(s)	160
PceI	AGGCCT	1 cut(s)	498
PciI	ACATGT	1 cut(s)	681
PdmI	GAANNNNTTC	1 cut(s)	967
PflMI	CCANNNNNTGG	1 cut(s)	982
PkrI	GCNGC	6 cut(s)	151, 291, 294, 747, 949, 952
PscI	ACATGT	1 cut(s)	681
PshBI	ATTAAT	1 cut(s)	365
Psp124BI	GAGCTC	1 cut(s)	76
Psp6I	CCWGG	1 cut(s)	461
PspFI	CCCAGC	1 cut(s)	285
PspGI	CCWGG	1 cut(s)	461
PspN4I	GGNNCC	1 cut(s)	801
PspPI	GGNCC	1 cut(s)	985
PsuI	RGATCY	1 cut(s)	922
RsaI	GTAC	1 cut(s)	894
RsaNI	GTAC	1 cut(s)	893
RseI	CAYNNNNRTG	2 cut(s)	162, 480
SacI	GAGCTC	1 cut(s)	76
SaqAI	TTAA	5 cut(s)	36, 365, 452, 609, 812
SatI	GCNGC	6 cut(s)	150, 290, 293, 746, 948, 951
Sau3AI	GATC	1 cut(s)	922
Sau96I	GGNCC	1 cut(s)	985
ScrFI	CCNGG	4 cut(s)	375, 376, 463, 722
SduI	GDGCHC	3 cut(s)	76, 587, 854
SfaNI	GCATC	3 cut(s)	210, 472, 494
SmaI	CCCGGG	1 cut(s)	376
SmiMI	CAYNNNNRTG	2 cut(s)	162, 480
SmlI	CTYRAG	1 cut(s)	864
SmoI	CTYRAG	1 cut(s)	864
SpeI	ACTAGT	1 cut(s)	755
Sse9I	AATT	6 cut(s)	241, 272, 366, 762, 928, 966
SseBI	AGGCCT	1 cut(s)	498
SspMI	CTAG	3 cut(s)	153, 567, 756
SstI	GAGCTC	1 cut(s)	76
StuI	AGGCCT	1 cut(s)	498
StyD4I	CCNGG	4 cut(s)	373, 374, 461, 720
StyI	CCWWGG	2 cut(s)	121, 566
TaaI	ACNGT	2 cut(s)	338, 885
TaiI	ACGT	3 cut(s)	14, 357, 878
TaqI	TCGA	2 cut(s)	70, 826
TasI	AATT	6 cut(s)	241, 272, 366, 762, 928, 966
Tru1I	TTAA	5 cut(s)	36, 365, 452, 609, 812
Tru9I	TTAA	5 cut(s)	36, 365, 452, 609, 812
TscAI	CASTG	2 cut(s)	343, 409
TseFI	GTSAC	2 cut(s)	544, 815
TseI	GCWGC	6 cut(s)	149, 289, 292, 745, 947, 950
Tsp45I	GTSAC	2 cut(s)	544, 815
TspDTI	ATGAA	2 cut(s)	411, 678
TspGWI	ACGGA	3 cut(s)	36, 757, 769
TspMI	CCCGGG	1 cut(s)	374
TspRI	CASTG	2 cut(s)	343, 409
Van91I	CCANNNNNTGG	1 cut(s)	982
VspI	ATTAAT	1 cut(s)	365
XapI	RAATTY	1 cut(s)	966
XceI	RCATGY	1 cut(s)	685
XmaI	CCCGGG	1 cut(s)	374
XmaJI	CCTAGG	1 cut(s)	566
XmnI	GAANNNNTTC	1 cut(s)	967
XspI	CTAG	3 cut(s)	153, 567, 756
Zsp2I	ATGCAT	2 cut(s)	133, 487

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.