Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0011530

Physical Location & Seq

Genomic Coordinates Reverse (-)

43952430 .. 43957641

Gene Structure 5212 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0289200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGCTGTCTCTCGTTTACACGACCCCAAAAGCCAATTCCACCTCTCCTCTCGTCAGCTTACCCCACTATTCTTCTTCTTCTCCTCCTTTTCACACTTCAACTCACCACACCCTATCTCTACCTGCTTCACCACGTCGCTCAACCAGGTTCAGAATGTCGTCCTCCGATTCGCTTCCCCCTCTCCCGGAAAACCGGACCGTGCTCGGTGTGGGCAGCATGGGGCTGGATTTCTTGGCGGTCGTGTCCTCTTATCCTCAGCCGGACGATAAGATCAGGACCACCAGCTTGAAGGTTCAAGGAGGTGGAAATGCCAGTAATGCTTTAACTTGTGTGGCTCGTTTGGGGCTAAGTCCCAGGGTCATTTCCAAGGTTGCGGATGATACACAAGGCAGGGGTATAATGGAGGAGCTACAAGCCGAAGGCGTAGATACTTCTTTTGTTGTGGTTGCTGAGGAGGGTAATTCACCATTTACCTACATTATTGTTGACAATGAAACGAATACTCGTACGTGTATTCATACTGAAGGATATCCTCCTATGATACCGGATGACCTTTCGCCTTCAAGTTTATCGTCTGCACTGGATGGAGCAAGACTTGCTTATTTTGATGGACGTCTACATGAAACTGCTTTACTTATTGCACAGGAGGCTACTCGCCGGAACATACCTATATTAATTGAGGCTGAAAGGATAAGAGAAGGGTTGGATGATCTTCTAAACTTGTCTGAGTATGCCATATGCTCAGCAAAATTTCCAAAGGCATGGACAGGAGCAGAAACTTTTCCAAGTGCTCTTGTTTCAATGCTTTTGAAATTGCCAAAGCTCAAATTTTCAATTGTGACCTTGGGTGAAGATGGATGCATAATGCTTGAGAGAAATATAGAAGAGGCTCCTCAGACGGAAGAAATGGACGTGGACAGCTTATTCGAAGCGCTGAAGCAGAGAAAGGATGACAGCATAACCAGACCAACATATGTTTCATCTCCAGTTACAAACTTGAGAGCCAATGGAATAGGGACAGTGTGTGGAAGGCTGCTTGTGGGAACAGCTGAGAAAATACCACCCTCTGAGCTTGTAGATACAACGGGTGCTGGAGATAGTTTTATTGGAGCAGTTATTTATGCTGTTTGCACCAACACGCCACCAGAGAAAATGCTCCCTTTTGCTTCTCAAGTGGCTGCTGCCTGCTGTAGAGGTTTGGGAGCACGAACTAGTCTTCCACACCGCACAGATCCACTCTTGGCACCATTTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0002237 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0009890 GO:0009892 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046677 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

418

Amino Acids

44.95

Weight (kDa)

5.3

Isoelectric Point (pI)

48.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	68 - 293	1.4e-21	pfkB family carbohydrate kinase
PfkB	PF00294	351 - 408	5.5e-13	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011530)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g41060 FvH4_2g41060 FvH4_2g41060
malus_domestica	MD00G1143500.v1.1 MD15G1039500.v1.1
prunus_persica	Prupe.1G393000_v2.0.a1
pyrus_communis	pycom08g04760 pycom15g03690
rosa_chinensis	RchiOBHm_Chr6g0299501
rosa_laevigata	RLG00000011426
rosa_multiflora	Rmu_sc0001184.1_g000004
rosa_roxburghii	Rroxscaffold_7G00168630
rosa_rugosa	Rorug06G0289200 Rorug06G0289200 Rorug06G0289300
rosa_samantha	Rh6AG400500 Rh6BG409200 Rh6CG414700 Rh6DG401600
rosa_wichuraiana	Rw6G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	616
Acc36I	ACCTGC	1 cut(s)	130
AccB1I	GGYRCC	1 cut(s)	1243
AccI	GTMKAC	1 cut(s)	616
AciI	CCGC	3 cut(s)	236, 374, 1225
AclWI	GGATC	1 cut(s)	1226
AcsI	RAATTY	2 cut(s)	749, 827
AcuI	CTGAAG	2 cut(s)	543, 956
AcyI	GRCGYC	1 cut(s)	613
AfaI	GTAC	1 cut(s)	508
AfeI	AGCGCT	1 cut(s)	933
AfiI	CCNNNNNNNGG	1 cut(s)	184
AflIII	ACRYGT	1 cut(s)	509
AgsI	TTSAA	7 cut(s)	99, 289, 296, 564, 801, 811, 834
AhlI	ACTAGT	1 cut(s)	1211
AjiI	CACGTC	2 cut(s)	134, 913
AjnI	CCWGG	2 cut(s)	143, 353
AjuI	GAANNNNNNNTTGG	4 cut(s)	19, 51, 961, 993
AloI	GAACNNNNNNTCC	2 cut(s)	1201, 1233
AluBI	AGCT	7 cut(s)	57, 285, 409, 823, 921, 1049, 1072
AluI	AGCT	7 cut(s)	57, 285, 409, 823, 921, 1049, 1072
Alw21I	GWGCWC	3 cut(s)	204, 793, 1207
Alw26I	GTCTC	1 cut(s)	12
AlwI	GGATC	1 cut(s)	1226
Aor51HI	AGCGCT	1 cut(s)	933
ApeKI	GCWGC	4 cut(s)	213, 1033, 1178, 1181
ApoI	RAATTY	2 cut(s)	749, 827
ArsI	GACNNNNNNTTYG	2 cut(s)	908, 940
AseI	ATTAAT	1 cut(s)	674
Asp700I	GAANNNNTTC	1 cut(s)	780
AspLEI	GCGC	1 cut(s)	934
AspS9I	GGNCC	2 cut(s)	195, 276
AsuC2I	CCSGG	1 cut(s)	185
AsuHPI	GGTGA	4 cut(s)	95, 120, 456, 860
AsuII	TTCGAA	1 cut(s)	927
AvaII	GGWCC	2 cut(s)	195, 276
BanI	GGYRCC	1 cut(s)	1243
BbsI	GAAGAC	1 cut(s)	1208
Bbv12I	GWGCWC	3 cut(s)	204, 793, 1207
BbvCI	CCTCAGC	2 cut(s)	255, 450
BbvI	GCAGC	4 cut(s)	225, 1020, 1165, 1168
BccI	CCATC	3 cut(s)	578, 602, 848
BciT130I	CCWGG	2 cut(s)	145, 355
BcnI	CCSGG	1 cut(s)	185
BcoDI	GTCTC	1 cut(s)	12
BcuI	ACTAGT	1 cut(s)	1211
BfaI	CTAG	1 cut(s)	1212
BfmI	CTRYAG	1 cut(s)	1189
BfoI	RGCGCY	1 cut(s)	935
BfuAI	ACCTGC	1 cut(s)	130
BisI	GCNGC	4 cut(s)	214, 1034, 1179, 1182
BlpI	GCTNAGC	1 cut(s)	742
BlsI	GCNGC	4 cut(s)	215, 1035, 1180, 1183
Bme1390I	CCNGG	3 cut(s)	145, 185, 355
Bme18I	GGWCC	2 cut(s)	195, 276
BmgBI	CACGTC	2 cut(s)	134, 913
BmgT120I	GGNCC	2 cut(s)	195, 276
BmiI	GGNNCC	2 cut(s)	891, 1245
BmrFI	CCNGG	3 cut(s)	145, 185, 355
BmsI	GCATC	1 cut(s)	848
BpiI	GAAGAC	1 cut(s)	1208
BpmI	CTGGAG	2 cut(s)	969, 1113
Bpu10I	CCTNAGC	2 cut(s)	255, 450
Bpu1102I	GCTNAGC	1 cut(s)	742
Bpu14I	TTCGAA	1 cut(s)	927
BpuEI	CTTGAG	3 cut(s)	890, 1018, 1155
BpuMI	CCSGG	1 cut(s)	185
BsaAI	YACGTR	1 cut(s)	510
BsaHI	GRCGYC	1 cut(s)	613
BsaJI	CCNNGG	4 cut(s)	353, 354, 366, 843
BsaWI	WCCGGW	2 cut(s)	192, 544
Bsc4I	CCNNNNNNNGG	1 cut(s)	184
Bse1I	ACTGG	3 cut(s)	312, 585, 986
BseBI	CCWGG	2 cut(s)	145, 355
BseDI	CCNNGG	4 cut(s)	353, 354, 366, 843
BseGI	GGATG	6 cut(s)	382, 553, 589, 712, 863, 955
BseLI	CCNNNNNNNGG	1 cut(s)	184
BseMII	CTCAG	7 cut(s)	269, 441, 717, 756, 908, 1041, 1059
BseNI	ACTGG	3 cut(s)	312, 585, 986
BseRI	GAGGAG	5 cut(s)	36, 72, 419, 467, 882
BseXI	GCAGC	4 cut(s)	225, 1020, 1165, 1168
BsgI	GTGCAG	1 cut(s)	561
Bsh1285I	CGRYCG	1 cut(s)	240
BshNI	GGYRCC	1 cut(s)	1243
BsiEI	CGRYCG	1 cut(s)	240
BsiHKAI	GWGCWC	3 cut(s)	204, 793, 1207
BsiSI	CCGG	5 cut(s)	185, 193, 260, 545, 658
BsiWI	CGTACG	1 cut(s)	506
BslFI	GGGAC	2 cut(s)	336, 1030
BslI	CCNNNNNNNGG	1 cut(s)	184
BsmAI	GTCTC	1 cut(s)	12
BsmFI	GGGAC	2 cut(s)	336, 1030
Bsp119I	TTCGAA	1 cut(s)	927
Bsp1286I	GDGCHC	3 cut(s)	204, 793, 1207
Bsp143I	GATC	3 cut(s)	270, 709, 1231
Bsp1720I	GCTNAGC	1 cut(s)	742
BspACI	CCGC	3 cut(s)	236, 374, 1225
BspCNI	CTCAG	7 cut(s)	268, 442, 718, 755, 907, 1042, 1060
BspLI	GGNNCC	2 cut(s)	891, 1245
BspMI	ACCTGC	1 cut(s)	130
BspPI	GGATC	1 cut(s)	1226
BspT104I	TTCGAA	1 cut(s)	927
BspT107I	GGYRCC	1 cut(s)	1243
BsrI	ACTGG	3 cut(s)	312, 585, 986
BssECI	CCNNGG	4 cut(s)	353, 354, 366, 843
BssMI	GATC	3 cut(s)	270, 709, 1231
BssNI	GRCGYC	1 cut(s)	613
BssT1I	CCWWGG	2 cut(s)	366, 843
Bst2UI	CCWGG	2 cut(s)	145, 355
Bst4CI	ACNGT	2 cut(s)	199, 1021
Bst6I	CTCTTC	1 cut(s)	879
BstACI	GRCGYC	1 cut(s)	613
BstAPI	GCANNNNNTGC	1 cut(s)	596
BstBAI	YACGTR	1 cut(s)	510
BstBI	TTCGAA	1 cut(s)	927
BstC8I	GCNNGC	1 cut(s)	1186
BstDEI	CTNAG	8 cut(s)	255, 347, 450, 726, 742, 894, 1050, 1068
BstF5I	GGATG	6 cut(s)	382, 553, 589, 712, 863, 955
BstH2I	RGCGCY	1 cut(s)	935
BstHHI	GCGC	1 cut(s)	934
BstKTI	GATC	3 cut(s)	273, 712, 1234
BstMAI	GTCTC	1 cut(s)	12
BstMBI	GATC	3 cut(s)	270, 709, 1231
BstMCI	CGRYCG	1 cut(s)	240
BstMWI	GCNNNNNNNGC	3 cut(s)	317, 596, 647
BstNI	CCWGG	2 cut(s)	145, 355
BstSCI	CCNGG	3 cut(s)	143, 183, 353
BstSFI	CTRYAG	1 cut(s)	1189
BstV1I	GCAGC	4 cut(s)	225, 1020, 1165, 1168
BstV2I	GAAGAC	1 cut(s)	1208
BstX2I	RGATCY	1 cut(s)	1231
BstXI	CCANNNNNNTGG	1 cut(s)	762
BstYI	RGATCY	1 cut(s)	1231
BtrI	CACGTC	2 cut(s)	134, 913
BtsCI	GGATG	6 cut(s)	382, 553, 589, 712, 863, 955
BtsIMutI	CAGTG	2 cut(s)	578, 1026
BveI	ACCTGC	1 cut(s)	130
Cac8I	GCNNGC	1 cut(s)	1186
CfoI	GCGC	1 cut(s)	934
Cfr13I	GGNCC	2 cut(s)	195, 276
CpoI	CGGWCCG	1 cut(s)	195
CsiI	ACCWGGT	1 cut(s)	143
Csp6I	GTAC	1 cut(s)	507
CspI	CGGWCCG	1 cut(s)	195
CviAII	CATG	3 cut(s)	217, 620, 762
CviQI	GTAC	1 cut(s)	507
DdeI	CTNAG	8 cut(s)	255, 347, 450, 726, 742, 894, 1050, 1068
DpnI	GATC	3 cut(s)	272, 711, 1233
DpnII	GATC	3 cut(s)	270, 709, 1231
Eam1104I	CTCTTC	1 cut(s)	879
EarI	CTCTTC	1 cut(s)	879
Eco130I	CCWWGG	2 cut(s)	366, 843
Eco32I	GATATC	1 cut(s)	530
Eco47I	GGWCC	2 cut(s)	195, 276
Eco47III	AGCGCT	1 cut(s)	933
Eco57I	CTGAAG	2 cut(s)	543, 956
EcoRII	CCWGG	2 cut(s)	143, 353
EcoRV	GATATC	1 cut(s)	530
EcoT14I	CCWWGG	2 cut(s)	366, 843
EcoT22I	ATGCAT	1 cut(s)	863
ErhI	CCWWGG	2 cut(s)	366, 843
FaeI	CATG	3 cut(s)	220, 623, 765
FalI	AAGNNNNNCTT	2 cut(s)	583, 615
FaqI	GGGAC	2 cut(s)	336, 1030
FatI	CATG	3 cut(s)	216, 619, 761
FauNDI	CATATG	2 cut(s)	737, 973
FblI	GTMKAC	1 cut(s)	616
Fnu4HI	GCNGC	4 cut(s)	214, 1034, 1179, 1182
FokI	GGATG	6 cut(s)	389, 560, 596, 719, 870, 962
Fsp4HI	GCNGC	4 cut(s)	214, 1034, 1179, 1182
FspBI	CTAG	1 cut(s)	1212
GlaI	GCGC	1 cut(s)	933
GluI	GCNGC	4 cut(s)	214, 1034, 1179, 1182
GsuI	CTGGAG	2 cut(s)	969, 1113
HaeII	RGCGCY	1 cut(s)	935
HapII	CCGG	5 cut(s)	185, 193, 260, 545, 658
HhaI	GCGC	1 cut(s)	934
Hin1I	GRCGYC	1 cut(s)	613
Hin1II	CATG	3 cut(s)	220, 623, 765
Hin6I	GCGC	1 cut(s)	932
HinP1I	GCGC	1 cut(s)	932
HincII	GTYRAC	1 cut(s)	487
HindII	GTYRAC	1 cut(s)	487
HinfI	GANTC	1 cut(s)	167
HpaII	CCGG	5 cut(s)	185, 193, 260, 545, 658
HphI	GGTGA	4 cut(s)	95, 120, 456, 860
Hpy166II	GTNNAC	4 cut(s)	16, 487, 617, 916
Hpy188I	TCNGA	5 cut(s)	152, 166, 727, 897, 1069
Hpy188III	TCNNGA	1 cut(s)	274
Hpy8I	GTNNAC	4 cut(s)	16, 487, 617, 916
Hpy99I	CGWCG	1 cut(s)	138
HpyAV	CCTTC	6 cut(s)	283, 413, 518, 570, 692, 1023
HpyCH4III	ACNGT	2 cut(s)	199, 1021
HpyCH4IV	ACGT	4 cut(s)	133, 509, 613, 912
HpyCH4V	TGCA	4 cut(s)	578, 641, 861, 1131
HpyF10VI	GCNNNNNNNGC	3 cut(s)	317, 596, 647
HpyF3I	CTNAG	8 cut(s)	255, 347, 450, 726, 742, 894, 1050, 1068
HpySE526I	ACGT	4 cut(s)	133, 509, 613, 912
Hsp92I	GRCGYC	1 cut(s)	613
Hsp92II	CATG	3 cut(s)	220, 623, 765
HspAI	GCGC	1 cut(s)	932
Kzo9I	GATC	3 cut(s)	270, 709, 1231
LmnI	GCTCC	7 cut(s)	406, 587, 770, 895, 1109, 1161, 1202
Lsp1109I	GCAGC	4 cut(s)	225, 1020, 1165, 1168
LweI	GCATC	1 cut(s)	848
MabI	ACCWGGT	1 cut(s)	143
MaeI	CTAG	1 cut(s)	1212
MaeII	ACGT	4 cut(s)	133, 509, 613, 912
MaeIII	GTNAC	2 cut(s)	838, 988
MalI	GATC	3 cut(s)	272, 711, 1233
MboI	GATC	3 cut(s)	270, 709, 1231
MboII	GAAGA	8 cut(s)	63, 66, 69, 704, 863, 896, 914, 1208
MfeI	CAATTG	1 cut(s)	834
MflI	RGATCY	1 cut(s)	1231
MhlI	GDGCHC	3 cut(s)	204, 793, 1207
MluCI	AATT	7 cut(s)	34, 460, 675, 749, 812, 827, 834
MmeI	TCCRAC	1 cut(s)	684
Mph1103I	ATGCAT	1 cut(s)	863
MroXI	GAANNNNTTC	1 cut(s)	780
MseI	TTAA	2 cut(s)	323, 674
MslI	CAYNNNNRTG	1 cut(s)	1252
MspA1I	CMGCKG	1 cut(s)	1049
MspI	CCGG	5 cut(s)	185, 193, 260, 545, 658
MspR9I	CCNGG	3 cut(s)	145, 185, 355
MunI	CAATTG	1 cut(s)	834
MvaI	CCWGG	2 cut(s)	145, 355
MwoI	GCNNNNNNNGC	3 cut(s)	317, 596, 647
NciI	CCSGG	1 cut(s)	185
NdeI	CATATG	2 cut(s)	737, 973
NdeII	GATC	3 cut(s)	270, 709, 1231
NlaIII	CATG	3 cut(s)	220, 623, 765
NlaIV	GGNNCC	2 cut(s)	891, 1245
NmuCI	GTSAC	1 cut(s)	838
NsiI	ATGCAT	1 cut(s)	863
NspV	TTCGAA	1 cut(s)	927
PasI	CCCWGGG	1 cut(s)	354
PdmI	GAANNNNTTC	1 cut(s)	780
PfeI	GAWTC	1 cut(s)	167
Pfl23II	CGTACG	1 cut(s)	506
PfoI	TCCNGGA	1 cut(s)	183
PkrI	GCNGC	4 cut(s)	215, 1035, 1180, 1183
Ppu21I	YACGTR	1 cut(s)	510
PshBI	ATTAAT	1 cut(s)	674
Psp6I	CCWGG	2 cut(s)	143, 353
PspGI	CCWGG	2 cut(s)	143, 353
PspLI	CGTACG	1 cut(s)	506
PspN4I	GGNNCC	2 cut(s)	891, 1245
PspPI	GGNCC	2 cut(s)	195, 276
PsuI	RGATCY	1 cut(s)	1231
PvuII	CAGCTG	1 cut(s)	1049
RsaI	GTAC	1 cut(s)	508
RsaNI	GTAC	1 cut(s)	507
RseI	CAYNNNNRTG	1 cut(s)	1252
Rsr2I	CGGWCCG	1 cut(s)	195
RsrII	CGGWCCG	1 cut(s)	195
SaqAI	TTAA	2 cut(s)	323, 674
SatI	GCNGC	4 cut(s)	214, 1034, 1179, 1182
Sau3AI	GATC	3 cut(s)	270, 709, 1231
Sau96I	GGNCC	2 cut(s)	195, 276
ScrFI	CCNGG	3 cut(s)	145, 185, 355
SduI	GDGCHC	3 cut(s)	204, 793, 1207
SexAI	ACCWGGT	1 cut(s)	143
SfaNI	GCATC	1 cut(s)	848
SfcI	CTRYAG	1 cut(s)	1189
SfuI	TTCGAA	1 cut(s)	927
SinI	GGWCC	2 cut(s)	195, 276
SmiMI	CAYNNNNRTG	1 cut(s)	1252
SmlI	CTYRAG	3 cut(s)	869, 997, 1170
SmoI	CTYRAG	3 cut(s)	869, 997, 1170
SpeI	ACTAGT	1 cut(s)	1211
Sse9I	AATT	7 cut(s)	34, 460, 675, 749, 812, 827, 834
SsiI	CCGC	3 cut(s)	236, 374, 1225
SspMI	CTAG	1 cut(s)	1212
StyD4I	CCNGG	3 cut(s)	143, 183, 353
StyI	CCWWGG	2 cut(s)	366, 843
TaaI	ACNGT	2 cut(s)	199, 1021
TaiI	ACGT	4 cut(s)	136, 512, 616, 915
TaqI	TCGA	1 cut(s)	927
TasI	AATT	7 cut(s)	34, 460, 675, 749, 812, 827, 834
TfiI	GAWTC	1 cut(s)	167
Tru1I	TTAA	2 cut(s)	323, 674
Tru9I	TTAA	2 cut(s)	323, 674
TscAI	CASTG	2 cut(s)	585, 1026
TseFI	GTSAC	1 cut(s)	838
TseI	GCWGC	4 cut(s)	213, 1033, 1178, 1181
Tsp45I	GTSAC	1 cut(s)	838
TspDTI	ATGAA	4 cut(s)	506, 507, 636, 969
TspGWI	ACGGA	1 cut(s)	914
TspRI	CASTG	2 cut(s)	585, 1026
VpaK11BI	GGWCC	2 cut(s)	195, 276
VspI	ATTAAT	1 cut(s)	674
XapI	RAATTY	2 cut(s)	749, 827
XmiI	GTMKAC	1 cut(s)	616
XmnI	GAANNNNTTC	1 cut(s)	780
XspI	CTAG	1 cut(s)	1212
ZraI	GACGTC	1 cut(s)	614
Zsp2I	ATGCAT	1 cut(s)	863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.