Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011530

Physical Location & Seq

Genomic Coordinates Forward (+)

2783151 .. 2784898

Gene Structure 1748 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1039500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 909 bp

ATGGACTCAACGTGGGTGAACACTTCTCTCGGCCTTAATCTTGTTCCGTTCTGCAGTGCCGATGATCATCAAGCTCCTGCCACTGTAGCTAGTCATCATGATGTGTTGGCTGAAGAGTTGAATAGGATAAGTGTGGAGAACAAGAAGCTGACTGAGATGCTAACTGCCCTCTGCGAGAATTACAACACCTTGCAATCCCATGTGAGAGAATTGATGATCACCAAGCAGCAGCAAAACAACTCATCTGATATTTATAGGAAGCGAAAGGCAGGCAGTGAAGACTACAACAACGTGATTGGATTAATTAGTCCCGGGACTAATACTGACACCAGCTCCTTCAGTGATGATCAAGAGTACTCATGCAAGAAGCCAAGGGAGCACGCCAACTTAAAGATTTCTAGGGTTTATGTTCGCACCGATGCATCCGACACACGCCTGATTGTGAAGGATGGATACCAATGGAGAAAATATGGTCAGAAGGTCACAAGAGATAACCCGTCACCTAGGGCTTACTACAAGTGTTCCTTTGCCCCAAGTTGCCCAGTTAAAAAGAAGGTGCAAAAAAGTGCTGAAAATCCATGCGTACTGGTAGCTACATATGAAGGAGAACACAACCACATGCACCCTGAAAGCCGAGCTGAAGTTACACTGATAGCCCCAGTATCTCCAAACCAGAACCAGCCTCCAATTTCCGTTTCACCATCCATGGCAAAGAGATCTGCACCTCCAACTTTTTCATGTGACATTAACCGGATAAGTCCCAATACTACTATGTGCCCCCAAGAAACTGAAGCTGCGACGTTCCAACAGTTTTTGGTACAACAAATGGCTTCTTCCTTGACCCAAGATCCCAATTTCACATCTGCTCTTGCTGCTGCCATTTCAGGAAGAATTTCAGACCACTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0002237 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0009890 GO:0009892 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046677 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

303

Amino Acids

33.6

Weight (kDa)

7.1

Isoelectric Point (pI)

46.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	149 - 206	7.9e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011530)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g41060 FvH4_2g41060 FvH4_2g41060
malus_domestica	MD00G1143500.v1.1 MD15G1039500.v1.1
prunus_persica	Prupe.1G393000_v2.0.a1
pyrus_communis	pycom08g04760 pycom15g03690
rosa_chinensis	RchiOBHm_Chr6g0299501
rosa_laevigata	RLG00000011426
rosa_multiflora	Rmu_sc0001184.1_g000004
rosa_roxburghii	Rroxscaffold_7G00168630
rosa_rugosa	Rorug06G0289200 Rorug06G0289200 Rorug06G0289300
rosa_samantha	Rh6AG400500 Rh6BG409200 Rh6CG414700 Rh6DG401600
rosa_wichuraiana	Rw6G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	1 cut(s)	842
AcsI	RAATTY	1 cut(s)	891
AcuI	CTGAAG	4 cut(s)	132, 322, 660, 810
AfaI	GTAC	3 cut(s)	356, 585, 819
AgsI	TTSAA	1 cut(s)	121
AjuI	GAANNNNNNNTTGG	2 cut(s)	679, 711
AluBI	AGCT	7 cut(s)	74, 89, 148, 333, 593, 638, 794
AluI	AGCT	7 cut(s)	74, 89, 148, 333, 593, 638, 794
Alw21I	GWGCWC	1 cut(s)	381
AlwI	GGATC	1 cut(s)	842
Ama87I	CYCGRG	1 cut(s)	311
AoxI	GGCC	1 cut(s)	31
ApeKI	GCWGC	5 cut(s)	226, 229, 794, 872, 875
ApoI	RAATTY	1 cut(s)	891
AseI	ATTAAT	1 cut(s)	302
Asp700I	GAANNNNTTC	1 cut(s)	892
AspA2I	CCTAGG	1 cut(s)	503
AsuC2I	CCSGG	2 cut(s)	312, 313
AsuHPI	GGTGA	4 cut(s)	28, 211, 492, 690
AvaI	CYCGRG	1 cut(s)	311
AvrII	CCTAGG	1 cut(s)	503
BaeGI	GKGCMC	1 cut(s)	779
BarI	GAAGNNNNNNTAC	2 cut(s)	437, 469
BbsI	GAAGAC	1 cut(s)	285
Bbv12I	GWGCWC	1 cut(s)	381
BbvI	GCAGC	5 cut(s)	238, 241, 781, 859, 862
BccI	CCATC	2 cut(s)	443, 709
BciVI	GTATCC	1 cut(s)	446
BclI	TGATCA	3 cut(s)	64, 216, 346
BcnI	CCSGG	2 cut(s)	312, 313
BfaI	CTAG	3 cut(s)	90, 399, 504
BfmI	CTRYAG	2 cut(s)	52, 84
BfuI	GTATCC	1 cut(s)	446
BglII	AGATCT	1 cut(s)	716
BisI	GCNGC	5 cut(s)	227, 230, 795, 873, 876
BlnI	CCTAGG	1 cut(s)	503
BlsI	GCNGC	5 cut(s)	228, 231, 796, 874, 877
BmcAI	AGTACT	1 cut(s)	356
Bme1390I	CCNGG	2 cut(s)	312, 313
BmeT110I	CYCGRG	1 cut(s)	311
BmrFI	CCNGG	2 cut(s)	312, 313
BmrI	ACTGGG	2 cut(s)	536, 653
BmsI	GCATC	3 cut(s)	147, 409, 431
BmuI	ACTGGG	2 cut(s)	536, 653
BpiI	GAAGAC	1 cut(s)	285
BpuMI	CCSGG	2 cut(s)	312, 313
BsaBI	GATNNNNATC	1 cut(s)	66
BsaJI	CCNNGG	4 cut(s)	311, 371, 503, 705
BsaWI	WCCGGW	1 cut(s)	750
BsaXI	ACNNNNNCTCC	2 cut(s)	317, 347
Bse1I	ACTGG	3 cut(s)	542, 591, 659
Bse8I	GATNNNNATC	1 cut(s)	66
BseDI	CCNNGG	4 cut(s)	311, 371, 503, 705
BseGI	GGATG	3 cut(s)	422, 454, 701
BseJI	GATNNNNATC	1 cut(s)	66
BseMII	CTCAG	1 cut(s)	144
BseNI	ACTGG	3 cut(s)	542, 591, 659
BseSI	GKGCMC	1 cut(s)	779
BseXI	GCAGC	5 cut(s)	238, 241, 781, 859, 862
BsgI	GTGCAG	1 cut(s)	705
BshFI	GGCC	1 cut(s)	33
BsiHKAI	GWGCWC	1 cut(s)	381
BsiHKCI	CYCGRG	1 cut(s)	311
BsiSI	CCGG	2 cut(s)	312, 751
BslFI	GGGAC	3 cut(s)	294, 328, 744
BsmFI	GGGAC	3 cut(s)	294, 328, 744
BsnI	GGCC	1 cut(s)	33
BsoBI	CYCGRG	1 cut(s)	311
Bsp1286I	GDGCHC	2 cut(s)	381, 779
Bsp143I	GATC	5 cut(s)	64, 216, 346, 716, 847
Bsp19I	CCATGG	1 cut(s)	705
BspANI	GGCC	1 cut(s)	33
BspCNI	CTCAG	1 cut(s)	145
BspHI	TCATGA	2 cut(s)	97, 905
BspMAI	CTGCAG	1 cut(s)	56
BspPI	GGATC	1 cut(s)	842
BsrI	ACTGG	3 cut(s)	542, 591, 659
BssECI	CCNNGG	4 cut(s)	311, 371, 503, 705
BssMI	GATC	5 cut(s)	64, 216, 346, 716, 847
BssT1I	CCWWGG	3 cut(s)	371, 503, 705
Bst4CI	ACNGT	2 cut(s)	85, 810
Bst6I	CTCTTC	1 cut(s)	108
BstC8I	GCNNGC	2 cut(s)	271, 381
BstDEI	CTNAG	1 cut(s)	153
BstDSI	CCRYGG	1 cut(s)	705
BstF5I	GGATG	3 cut(s)	422, 454, 701
BstKTI	GATC	5 cut(s)	67, 219, 349, 719, 850
BstMBI	GATC	5 cut(s)	64, 216, 346, 716, 847
BstMWI	GCNNNNNNNGC	3 cut(s)	86, 376, 872
BstNSI	RCATGY	1 cut(s)	622
BstSCI	CCNGG	2 cut(s)	310, 311
BstSFI	CTRYAG	2 cut(s)	52, 84
BstSLI	GKGCMC	1 cut(s)	779
BstV1I	GCAGC	5 cut(s)	238, 241, 781, 859, 862
BstV2I	GAAGAC	1 cut(s)	285
BstX2I	RGATCY	2 cut(s)	716, 847
BstYI	RGATCY	2 cut(s)	716, 847
BsuI	GTATCC	1 cut(s)	446
BsuRI	GGCC	1 cut(s)	33
BtgI	CCRYGG	1 cut(s)	705
BtsCI	GGATG	3 cut(s)	422, 454, 701
BtsI	GCAGTG	2 cut(s)	61, 280
BtsIMutI	CAGTG	5 cut(s)	61, 81, 280, 346, 647
Cac8I	GCNNGC	2 cut(s)	271, 381
CciI	TCATGA	2 cut(s)	97, 905
Cfr9I	CCCGGG	1 cut(s)	311
Csp6I	GTAC	3 cut(s)	355, 584, 818
CviAII	CATG	8 cut(s)	98, 200, 360, 579, 619, 706, 738, 906
CviQI	GTAC	3 cut(s)	355, 584, 818
DdeI	CTNAG	1 cut(s)	153
DpnI	GATC	5 cut(s)	66, 218, 348, 718, 849
DpnII	GATC	5 cut(s)	64, 216, 346, 716, 847
Eam1104I	CTCTTC	1 cut(s)	108
EarI	CTCTTC	1 cut(s)	108
Eco130I	CCWWGG	3 cut(s)	371, 503, 705
Eco57I	CTGAAG	4 cut(s)	132, 322, 660, 810
Eco88I	CYCGRG	1 cut(s)	311
EcoT14I	CCWWGG	3 cut(s)	371, 503, 705
EcoT22I	ATGCAT	1 cut(s)	424
ErhI	CCWWGG	3 cut(s)	371, 503, 705
FaeI	CATG	8 cut(s)	101, 203, 363, 582, 622, 709, 741, 909
FalI	AAGNNNNNCTT	2 cut(s)	509, 541
FaqI	GGGAC	3 cut(s)	294, 328, 744
FatI	CATG	8 cut(s)	97, 199, 359, 578, 618, 705, 737, 905
FauNDI	CATATG	1 cut(s)	598
FbaI	TGATCA	3 cut(s)	64, 216, 346
Fnu4HI	GCNGC	5 cut(s)	227, 230, 795, 873, 876
FokI	GGATG	3 cut(s)	409, 461, 688
Fsp4HI	GCNGC	5 cut(s)	227, 230, 795, 873, 876
FspBI	CTAG	3 cut(s)	90, 399, 504
GluI	GCNGC	5 cut(s)	227, 230, 795, 873, 876
HaeIII	GGCC	1 cut(s)	33
HapII	CCGG	2 cut(s)	312, 751
Hin1II	CATG	8 cut(s)	101, 203, 363, 582, 622, 709, 741, 909
HinfI	GANTC	1 cut(s)	5
HpaII	CCGG	2 cut(s)	312, 751
HphI	GGTGA	4 cut(s)	28, 211, 492, 690
Hpy166II	GTNNAC	1 cut(s)	19
Hpy188I	TCNGA	4 cut(s)	247, 427, 477, 898
Hpy188III	TCNNGA	4 cut(s)	98, 350, 885, 906
Hpy8I	GTNNAC	1 cut(s)	19
Hpy99I	CGWCG	1 cut(s)	802
HpyAV	CCTTC	5 cut(s)	346, 439, 472, 547, 596
HpyCH4III	ACNGT	2 cut(s)	85, 810
HpyCH4IV	ACGT	3 cut(s)	11, 291, 800
HpyCH4V	TGCA	7 cut(s)	54, 193, 363, 422, 559, 622, 722
HpyF10VI	GCNNNNNNNGC	3 cut(s)	86, 376, 872
HpyF3I	CTNAG	1 cut(s)	153
HpySE526I	ACGT	3 cut(s)	11, 291, 800
Hsp92II	CATG	8 cut(s)	101, 203, 363, 582, 622, 709, 741, 909
Ksp22I	TGATCA	3 cut(s)	64, 216, 346
Kzo9I	GATC	5 cut(s)	64, 216, 346, 716, 847
LmnI	GCTCC	3 cut(s)	79, 338, 376
Lsp1109I	GCAGC	5 cut(s)	238, 241, 781, 859, 862
LweI	GCATC	3 cut(s)	147, 409, 431
MaeI	CTAG	3 cut(s)	90, 399, 504
MaeII	ACGT	3 cut(s)	11, 291, 800
MaeIII	GTNAC	4 cut(s)	481, 498, 643, 740
MalI	GATC	5 cut(s)	66, 218, 348, 718, 849
MboI	GATC	5 cut(s)	64, 216, 346, 716, 847
MboII	GAAGA	4 cut(s)	125, 290, 825, 900
MflI	RGATCY	2 cut(s)	716, 847
MhlI	GDGCHC	2 cut(s)	381, 779
MluCI	AATT	6 cut(s)	178, 209, 303, 687, 853, 891
MmeI	TCCRAC	3 cut(s)	450, 752, 829
MnlI	CCTC	3 cut(s)	179, 693, 735
Mph1103I	ATGCAT	1 cut(s)	424
MroXI	GAANNNNTTC	1 cut(s)	892
MseI	TTAA	5 cut(s)	36, 302, 389, 546, 747
MslI	CAYNNNNRTG	1 cut(s)	99
MspI	CCGG	2 cut(s)	312, 751
MspR9I	CCNGG	2 cut(s)	312, 313
MwoI	GCNNNNNNNGC	3 cut(s)	86, 376, 872
NciI	CCSGG	2 cut(s)	312, 313
NcoI	CCATGG	1 cut(s)	705
NdeI	CATATG	1 cut(s)	598
NdeII	GATC	5 cut(s)	64, 216, 346, 716, 847
NlaIII	CATG	8 cut(s)	101, 203, 363, 582, 622, 709, 741, 909
NmeAIII	GCCGAG	2 cut(s)	9, 659
NmuCI	GTSAC	3 cut(s)	481, 498, 740
NsiI	ATGCAT	1 cut(s)	424
NspI	RCATGY	1 cut(s)	622
PagI	TCATGA	2 cut(s)	97, 905
PdmI	GAANNNNTTC	1 cut(s)	892
PkrI	GCNGC	5 cut(s)	228, 231, 796, 874, 877
PshBI	ATTAAT	1 cut(s)	302
PstI	CTGCAG	1 cut(s)	56
PsuI	RGATCY	2 cut(s)	716, 847
RsaI	GTAC	3 cut(s)	356, 585, 819
RsaNI	GTAC	3 cut(s)	355, 584, 818
RseI	CAYNNNNRTG	1 cut(s)	99
SaqAI	TTAA	5 cut(s)	36, 302, 389, 546, 747
SatI	GCNGC	5 cut(s)	227, 230, 795, 873, 876
Sau3AI	GATC	5 cut(s)	64, 216, 346, 716, 847
ScaI	AGTACT	1 cut(s)	356
ScrFI	CCNGG	2 cut(s)	312, 313
SduI	GDGCHC	2 cut(s)	381, 779
SfaNI	GCATC	3 cut(s)	147, 409, 431
SfcI	CTRYAG	2 cut(s)	52, 84
SmaI	CCCGGG	1 cut(s)	313
SmiMI	CAYNNNNRTG	1 cut(s)	99
Sse9I	AATT	6 cut(s)	178, 209, 303, 687, 853, 891
SspMI	CTAG	3 cut(s)	90, 399, 504
StyD4I	CCNGG	2 cut(s)	310, 311
StyI	CCWWGG	3 cut(s)	371, 503, 705
TaaI	ACNGT	2 cut(s)	85, 810
TaiI	ACGT	3 cut(s)	14, 294, 803
TasI	AATT	6 cut(s)	178, 209, 303, 687, 853, 891
TatI	WGTACW	1 cut(s)	354
Tru1I	TTAA	5 cut(s)	36, 302, 389, 546, 747
Tru9I	TTAA	5 cut(s)	36, 302, 389, 546, 747
TscAI	CASTG	5 cut(s)	61, 88, 280, 346, 654
TseFI	GTSAC	3 cut(s)	481, 498, 740
TseI	GCWGC	5 cut(s)	226, 229, 794, 872, 875
Tsp45I	GTSAC	3 cut(s)	481, 498, 740
TspDTI	ATGAA	2 cut(s)	615, 726
TspGWI	ACGGA	2 cut(s)	36, 682
TspMI	CCCGGG	1 cut(s)	311
TspRI	CASTG	5 cut(s)	61, 88, 280, 346, 654
VspI	ATTAAT	1 cut(s)	302
XapI	RAATTY	1 cut(s)	891
XceI	RCATGY	1 cut(s)	622
XmaI	CCCGGG	1 cut(s)	311
XmaJI	CCTAGG	1 cut(s)	503
XmnI	GAANNNNTTC	1 cut(s)	892
XspI	CTAG	3 cut(s)	90, 399, 504
ZrmI	AGTACT	1 cut(s)	356
Zsp2I	ATGCAT	1 cut(s)	424

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.