MD00G1149200.v1.1

Transcriptional regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010370
Physical Location & Seq
Forward (+)
32820914 .. 32831162
10249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1149200.v1.1.491

Sequence Viewer

Length: 4338 bp
ATGGAGGAAAGCCACGAGCAAGTGGGGGAGCAAATGGAGGAAAACGGCGAGCAAGTGGAAAATATTGGGAGTGCTTCTAGTGGCAGTGATAGTGATTCTTTCATAGATGACTCAGAAGTTGATGAAGTACCGATATCCGGCGAAGATGGAAAATTGCAGCCAGAGGAACCATTATCTGATAAAGAAATAGAGGAGCTTATTGCTGAATTTTTGGAGGTTGAGAGTAAGGCTGCAGAGGCTCAAGAGGCACTTGAGAAAGAGTCTCTTGCCAAGGTGGAGAGTGAAGTAAGAGAGGAGTTGGCACAAACCCTTCATGGAGATGATTTGGAAGCAGCTGTTGCGAATGAAATGGATACTTTGATAGAAGAGTGGCAAGCCGAGCTTGATGAACTTGAGACTGAGAGTGCTCATTTGTTGGAACAACTTGATGGGGCCGGCATTGAGCTACCAAGCCTTTATAAATGCATTGAAAGTCAGGCTCCTAATGGTTGCTGCACTGAAGCTTGGAAAAGACGGATACACTGGGTAGGATCTCAAGAGACTGGCGAATTTACCGAGTCAAGGGCTGATGCAGAGAAGTATCTCCAAACCCACAGGCCTGTGAGAAGGCGACATGGTAAACTATTGGAGGATGGCGCTAGTGGATTTCTGCAGAAAAAACTAGTCGTAGATGGAATCAAGGATGTTGAGACAGCTGAGGTTGATTACTGGGGTTCTTTTAACAAATTATTTTCTGATGGTGCAACTGCGGGTGGTGCCTCGTTTGGCAGTAAGCACTGGGCTTCTGTTTATTTGGCCAGTACGCCACAGCAAGCTGCTGAGATGGGACTCAAGTTCCCTGGGGTTGATGAGGTAGAGGAGATTGATGACATTGATGGCAATTCGGGTGATCCATTTGTTGCAGACGCTGTTGCAAACGAAAGAGAACTGGATCTCACTGAAGAACAAAAGAAAAATTATAGGAAGGTCAAAGAAGAAGATGATGTAAATGTTGATCGGAAGCTTCAAATTCGTTTGAAACGAAGGAGACATCGAAAGAGACGTAAACAGTTCGAGAAAATTGATGAAGATCTGGAAATCGCTAATAACATTGACCAAGAAAGCATAACGAGTAACGGTGCATCTCCGGTGACTAGTTCCAGTGAAGCAAGAGGCTCCAAGCGTCTGAGTGAGGATGAGGAGCTAAACATTGATAATAAGAGGAGTCGAACTGTACTAATAGATAGTGATGATGACACTCCACTGAAAGATAATTCAGACTGCAATGCAATAAAATCCGAGGACCAGTCCTATGTGGAAGAAAATATCTGCATATCTGCCAGTGGTGGTCTTCCTTCACAGAGTCTGAATGATAAGCTCTACTGCACTGCTTGCAGTAAGCATGCTCTCAAAGTGTGTTCACATCCACTTCTGAAGGTGATTGTTTGTGCAGATTGCAGATGTTTATTGGAAGAAAAGATGCATGTGAAGGATCCTGATTGCACTAAGTGTTACTGTGGGTGGTGTGGACAAAGCAAGGACCTAGTAAATTGTAACTCTTGCAAGACGTTGGTTTGTACAACTTGTATAAAGAGGAATATTGGAGAGGAATGCTTATCTGATGCCCAGACCTCTGGCTGGCAATGTTGTTTCTGTTGCCCAAGTCTTCTAAAGACATTGACGTCACAATTAGAGCAAGCAATAGGTTGTGGGGATTTGATAGATTCTAGCTCTGATAGTGATTCAGATAGTTCAGGTTCAGAGATAGATGTTACTGTAAGTTCTAAGAGGAGGAGAAAGCAGAAAATTCGAAGGATCATTGATGACACTGAATTAGGAGAAGAAACCAGAAGGAAAATTGCAATTGAAAAGGAACGTCAAGAGCGCTTGATGTCTTTGCAAGTACAATTTTCTGCCAAATCTAAGATGAAGAGCTCTGCAACCTGTAATGGGAGATTACCCGAAGGCGCCAACACTGAAGTGCTAGGTGATGCATCGGTTGGTTATGTAGTGAATGTTGTGAGGGAGAAAGGTGAAGAAGCTGTCAGAATTCCTCCAAGCATCTCAGCTAAATTGAAGGCTCATCAGATTACGGGTGTGAGATTTATGTGGGAGAATATCATACAGTCAGTCAGAAAAGTGAAGGCTGGGGATAAAGGTCTTGGTTGCATTCTAGCTCACATGATGGGCCTTGGTAAAACTTTTCAGGTCATAGCTTTTTTGTACACTGCAATGAGAAGTATTGATTTGGGATTAAAAACCGCACTTATTGTCACCCCTGTGAACGTGCTGCACAATTGGCGTCAAGAGTTCATGAAGTGGAGACCTTCAGAACTAAAGCCTCTTCGCATCTTCATGCTAGAAGATGTGTCAAGGGATAGAAGAGCAGAGTTGCTTGCAAAGTGGAGAAGGAAGGGTGGTGTTTTTCTAATTGGCTACTCTGCTTTTAGAAACTTATCCTTTGGGAAGCATGTGAAGGATAGGCAGATAGCTACAGAAATTTGTCGTGCCTTACAGGATGGACCGGATATACTTGTTTGTGATGAGGCCCATGTTATTAAGAATACCAGGGCTGATGTAACCCAAGCCTTAAAACAAGTGAAATGCCAGAGAAGGATAGCATTAACTGGATCACCACTTCAGAACAATCTCATGGAATATTATTGTATGGTTGATTTTGTAAGGGAAGGTTTTCTTGGAAGCAGCCATGAGTTTCGGAATCGATTCCAAAATCCCATAGAGTACGGTCAGCATACTAATTCAACCGTGGATGATGTGAAAATCATGAACCAACGGTCTCATATTCTTTATGAACAGTTGAAAGGATTTGTTCAGAGAATGGACATGACTGTTGTGAAGAAGGACTTACCACCAAAAACTGTGTTTGTAATAGCTGTGAAACTTTCAACCTTACAGAGGAAATTATACAAGAGATTTCTTGATGTCCATGGATTTACCAAGGACAATGATTATAATGAAAAGATAGGGAAGAGAAGTTTTTTTGCCGGCTACCAGGCCTTAGCTCAGATATGGAACCATCCGGGGATTGTGCAACTAAGAAAAGACAACAAAGACTATGAGAGAAGTGGAGATGCCGTTGAGAATTTTCTTGCAGATGACAGCTCTAGTGACGAAAACATTGATTATAATTTGGGTTTGGGAGAGAAAAATGTGAATGAAATTTTGCCAGGAAAAAAAGATGGCATTTTTCATAAGAATTGGTGGAATGATCTTCTTCATGAAAATAACTATAAAGAGCTTGATTACAGTGGCAAAATGGTACTCCTGCTTGACATTCTAGCTATGAGTTCTGATGTGGGCGATAAGGCATTGGTATTTAGCCAGAGCATACCAACCCTAGACCTTATTGAACTTTATCTTTCAAGGCTACCTCGACATGGGAAGAAAGGGAAGTCCTGGAAGAAAGGAAAAGATTGGTACAGGCTAGATGGGAGAACAGAGGGCTCAGAAAGGCAAAAGCTGGTTGAACGCTTTAATGATCCCCTAAATAAGAGGGTGAAATGTGTTATAATTTCCACCAGAGCTGGATCATTAGGGATAAATCTATATGCTGCTAATCGCGTAATAATAGTTGATGGTTCTTGGAATCCAACATACGATCTTCAGGCTATATATCGTGCTTGGAGGTATGGCCAAACAAAGCCAGTGTTCGCTTACAGATTGATGGCACATGGAACTATGGAAGAAAAAATTTATAAGCGTCAGGTAACGAAGGAGGGCCTGGCTGCAAGGGTTGTTGACCGACAACAGGTACATAGGACAATATCTAAGGAAGAGATGTTGCATCTTTTTGAATTTGGTGATGATGAGAACCATGAGCTTGACCAAGAAAACGGACGCATGAACGACAATATGACTGGTGAAGTGGAAATTTTGCCAAAACACGTAGTTCCTCTTTCTCAAGGAAGTTGCTCTTCTGACAAGCTCATGGAAAGGTTAATTGGCAAGCATTCTCCAAGGTGGATCGCAAATTTCCACGAACATGAGACCCTTTTGCAAGAGAATGAAGAAGAAAGGCTCACAAAGGAGGAGCAAGACATGGCCTGGGAAGTATACCAGAAATCATTTGGATGGGAGGAAGTGCAGAGAATTCCTCTCAAAGAATCTGCAATCGACCAAAAACCAGCTGCATCAAATACTGCGTCATCTGCACCAAAGAAAAGCATCCTTGCTGAATCCAAGGCGAAGAATGCTTTTGTGCAGCGGAAGTGCACTAACCTTTCGCATTTGCTGACGCTAAGAAGTCAGGGCACAAAACAGGGTTGCACTACTGTGTGTGGGGAATGTGGCCGCGAGCTAAGCTGGGAGGAGCACAATCGAGATAGCAGGCTCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000775 GO:0000785 GO:0000792 GO:0000902 GO:0000904 GO:0001674 GO:0001932 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005700 GO:0005701 GO:0005721 GO:0005722 GO:0006139 GO:0006259 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006915 GO:0006928 GO:0006935 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0008150 GO:0008152 GO:0008219 GO:0008347 GO:0009555 GO:0009605 GO:0009653 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009987 GO:0010369 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0012501 GO:0016043 GO:0016458 GO:0016462 GO:0016477 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0022008 GO:0022607 GO:0023051 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0031175 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031399 GO:0031497 GO:0032268 GO:0032501 GO:0032502 GO:0032872 GO:0032989 GO:0032990 GO:0033043 GO:0033044 GO:0034401 GO:0034622 GO:0034641 GO:0034724 GO:0034728 GO:0035556 GO:0040011 GO:0040029 GO:0042063 GO:0042221 GO:0042325 GO:0042330 GO:0042393 GO:0042585 GO:0043007 GO:0043073 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043408 GO:0043570 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046328 GO:0046483 GO:0048229 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048870 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051252 GO:0051253 GO:0051276 GO:0051674 GO:0051716 GO:0060255 GO:0061564 GO:0065003 GO:0065004 GO:0065007 GO:0070302 GO:0070828 GO:0070868 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097190 GO:0097193 GO:0097485 GO:0097549 GO:0098687 GO:0120036 GO:0120039 GO:1900049 GO:1901360 GO:1902275 GO:1902531 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1446

Amino Acids

163.51

Weight (kDa)

5.59

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 688 - 1008 1.7e-53 SNF2-related domain
ResIII PF04851 697 - 872 5.5e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 1085 - 1211 1.7e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08600 AT1G08600 AT1G08600 AT1G08600
fragaria_vesca FvH4_6g01581
malus_domestica MD00G1149200.v1.1 MD04G1232200.v1.1
prunus_persica Prupe.6G351600_v2.0.a1 Prupe.6G351600_v2.0.a1
pyrus_communis pycom04g20560 pycom12g22990
rosa_chinensis RchiOBHm_Chr3g0449401
rosa_laevigata RLG00000025834
rosa_multiflora Rmu_ssc0000423.1_g000001
rosa_roxburghii Rroxscaffold_6G00427360
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018100 Rh3BG018400 Rh3CG017300 Rh3DG018800
rosa_wichuraiana Rw3G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 459, 2952, 3126, 3509, 3696
AasI GACNNNNNNGTC 1 cut(s) 1660
AatII GACGTC 1 cut(s) 1664
AccB1I GGYRCC 2 cut(s) 755, 1946
AccI GTMKAC 1 cut(s) 4053
AccII CGCG 2 cut(s) 3561, 4293
AciI CCGC 4 cut(s) 749, 2241, 4204, 4291
AcoI YGGCCR 3 cut(s) 795, 3631, 4288
AcuI CTGAAG 7 cut(s) 519, 960, 1433, 1977, 2292, 2603, 3587
AcyI GRCGYC 3 cut(s) 1661, 1947, 2281
AdeI CACNNNGTG 1 cut(s) 1488
AfeI AGCGCT 1 cut(s) 1865
AfiI CCNNNNNNNGG 7 cut(s) 137, 561, 1617, 1929, 2895, 3377, 3748
AflIII ACRYGT 1 cut(s) 3883
AhlI ACTAGT 2 cut(s) 661, 1133
AjnI CCWGG 7 cut(s) 838, 2546, 2991, 3166, 3395, 3720, 4043
AjuI GAANNNNNNNTTGG 4 cut(s) 2658, 2690, 3924, 3956
AleI CACNNNNGTG 3 cut(s) 1958, 2258, 4271
Alw21I GWGCWC 4 cut(s) 409, 1916, 4214, 4314
Alw26I GTCTC 9 cut(s) 267, 389, 533, 683, 1021, 1033, 2296, 2781, 3980
Alw44I GTGCAC 1 cut(s) 4210
AlwNI CAGNNNCTG 2 cut(s) 908, 1345
Aor51HI AGCGCT 1 cut(s) 1865
ApaLI GTGCAC 1 cut(s) 4210
ArsI GACNNNNNNTTYG 2 cut(s) 4127, 4159
Asp700I GAANNNNTTC 2 cut(s) 2670, 2702
AspLEI GCGC 3 cut(s) 638, 1866, 1949
AspS9I GGNCC 7 cut(s) 432, 1282, 1519, 2167, 2501, 2527, 3718
AsuC2I CCSGG 1 cut(s) 3021
AsuII TTCGAA 1 cut(s) 1789
AvaII GGWCC 3 cut(s) 1282, 1519, 2501
BaeGI GKGCMC 2 cut(s) 4214, 4253
BalI TGGCCA 2 cut(s) 797, 3633
BamHI GGATCC 1 cut(s) 1471
BanI GGYRCC 2 cut(s) 755, 1946
BanII GRGCYC 2 cut(s) 1916, 3446
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 1322, 1637
Bbv12I GWGCWC 4 cut(s) 409, 1916, 4214, 4314
BbvCI CCTCAGC 1 cut(s) 696
BceAI ACGGC 2 cut(s) 61, 3059
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciT130I CCWGG 7 cut(s) 840, 2548, 2993, 3168, 3397, 3722, 4045
BciVI GTATCC 2 cut(s) 346, 510
BcnI CCSGG 1 cut(s) 3021
BcoDI GTCTC 9 cut(s) 267, 389, 533, 683, 1021, 1033, 2296, 2781, 3980
BcuI ACTAGT 2 cut(s) 661, 1133
BfmI CTRYAG 3 cut(s) 231, 650, 2472
BfoI RGCGCY 3 cut(s) 639, 1867, 1950
BfuI GTATCC 2 cut(s) 346, 510
BglII AGATCT 1 cut(s) 1069
BlpI GCTNAGC 1 cut(s) 4298
Bme1390I CCNGG 8 cut(s) 840, 2548, 2993, 3021, 3168, 3397, 3722, 4045
Bme18I GGWCC 3 cut(s) 1282, 1519, 2501
BmgT120I GGNCC 7 cut(s) 432, 1282, 1519, 2167, 2501, 2527, 3718
BmiI GGNNCC 8 cut(s) 168, 433, 480, 757, 1156, 1473, 1948, 3014
BmrFI CCNGG 8 cut(s) 840, 2548, 2993, 3021, 3168, 3397, 3722, 4045
BmrI ACTGGG 3 cut(s) 532, 718, 787
BmuI ACTGGG 3 cut(s) 532, 718, 787
BpiI GAAGAC 2 cut(s) 1322, 1637
BplI GAGNNNNNCTC 2 cut(s) 4078, 4110
Bpu10I CCTNAGC 3 cut(s) 696, 2998, 4331
Bpu1102I GCTNAGC 1 cut(s) 4298
Bpu14I TTCGAA 1 cut(s) 1789
BpuEI CTTGAG 6 cut(s) 225, 272, 413, 519, 815, 3885
BpuMI CCSGG 1 cut(s) 3021
Bsa29I ATCGAT 1 cut(s) 2701
BsaAI YACGTR 1 cut(s) 3886
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 3 cut(s) 1661, 1947, 2281
BsaI GGTCTC 3 cut(s) 2296, 2781, 3980
BsaWI WCCGGW 2 cut(s) 1126, 2503
BsaXI ACNNNNNCTCC 2 cut(s) 3060, 3090
Bsc4I CCNNNNNNNGG 7 cut(s) 137, 561, 1617, 1929, 2895, 3377, 3748
Bse118I RCCGGY 2 cut(s) 434, 2984
Bse3DI GCAATG 3 cut(s) 1270, 1628, 2217
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 7 cut(s) 840, 2548, 2993, 3168, 3397, 3722, 4045
BseCI ATCGAT 1 cut(s) 2701
BseGI GGATG 9 cut(s) 637, 688, 1180, 1402, 2503, 2755, 3016, 4076, 4164
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 7 cut(s) 137, 561, 1617, 1929, 2895, 3377, 3748
BseMI GCAATG 3 cut(s) 1270, 1628, 2217
BseMII CTCAG 8 cut(s) 126, 390, 687, 810, 1157, 2058, 3017, 3459
BseRI GAGGAG 9 cut(s) 206, 308, 872, 1193, 1216, 1783, 1786, 4043, 4322
BseSI GKGCMC 2 cut(s) 4214, 4253
BseYI CCCAGC 2 cut(s) 2126, 4302
BsgI GTGCAG 7 cut(s) 478, 1348, 1449, 2255, 4103, 4134, 4220
Bsh1236I CGCG 2 cut(s) 3561, 4293
BshNI GGYRCC 2 cut(s) 755, 1946
BshVI ATCGAT 1 cut(s) 2701
BsiHKAI GWGCWC 4 cut(s) 409, 1916, 4214, 4314
BsiSI CCGG 6 cut(s) 138, 435, 1127, 2504, 2985, 3020
BslFI GGGAC 1 cut(s) 840
BslI CCNNNNNNNGG 7 cut(s) 137, 561, 1617, 1929, 2895, 3377, 3748
BsmAI GTCTC 9 cut(s) 267, 389, 533, 683, 1021, 1033, 2296, 2781, 3980
BsmBI CGTCTC 1 cut(s) 1033
BsmFI GGGAC 1 cut(s) 840
BsmI GAATGC 4 cut(s) 1595, 2148, 3949, 4195
Bso31I GGTCTC 3 cut(s) 2296, 2781, 3980
Bsp119I TTCGAA 1 cut(s) 1789
Bsp1286I GDGCHC 6 cut(s) 409, 1916, 3446, 4214, 4253, 4314
Bsp1407I TGTACA 2 cut(s) 1556, 2202
Bsp1720I GCTNAGC 1 cut(s) 4298
Bsp19I CCATGG 1 cut(s) 2926
BspACI CCGC 4 cut(s) 749, 2241, 4204, 4291
BspCNI CTCAG 8 cut(s) 125, 391, 688, 811, 1158, 2057, 3016, 3458
BspDI ATCGAT 1 cut(s) 2701
BspFNI CGCG 2 cut(s) 3561, 4293
BspHI TCATGA 3 cut(s) 2292, 2763, 3217
BspLI GGNNCC 8 cut(s) 168, 433, 480, 757, 1156, 1473, 1948, 3014
BspMAI CTGCAG 2 cut(s) 235, 654
BspQI GCTCTTC 3 cut(s) 1904, 2356, 3919
BspT104I TTCGAA 1 cut(s) 1789
BspT107I GGYRCC 2 cut(s) 755, 1946
BspTNI GGTCTC 3 cut(s) 2296, 2781, 3980
BsrDI GCAATG 3 cut(s) 1270, 1628, 2217
BsrFI RCCGGY 2 cut(s) 434, 2984
BsrGI TGTACA 2 cut(s) 1556, 2202
BssAI RCCGGY 2 cut(s) 434, 2984
BssNAI GTATAC 1 cut(s) 4054
BssNI GRCGYC 3 cut(s) 1661, 1947, 2281
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 6 cut(s) 270, 2170, 2926, 2937, 3956, 4179
Bst1107I GTATAC 1 cut(s) 4054
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 7 cut(s) 840, 2548, 2993, 3168, 3397, 3722, 4045
Bst6I CTCTTC 7 cut(s) 360, 1904, 2328, 2356, 2963, 3768, 3919
BstACI GRCGYC 3 cut(s) 1661, 1947, 2281
BstAPI GCANNNNNTGC 2 cut(s) 338, 1371
BstAUI TGTACA 2 cut(s) 1556, 2202
BstBAI YACGTR 1 cut(s) 3886
BstBI TTCGAA 1 cut(s) 1789
BstDSI CCRYGG 2 cut(s) 2745, 2926
BstENI CCTNNNNNAGG 1 cut(s) 2893
BstF5I GGATG 9 cut(s) 637, 688, 1180, 1402, 2503, 2755, 3016, 4076, 4164
BstFNI CGCG 2 cut(s) 3561, 4293
BstH2I RGCGCY 3 cut(s) 639, 1867, 1950
BstHHI GCGC 3 cut(s) 638, 1866, 1949
BstMAI GTCTC 9 cut(s) 267, 389, 533, 683, 1021, 1033, 2296, 2781, 3980
BstNI CCWGG 7 cut(s) 840, 2548, 2993, 3168, 3397, 3722, 4045
BstNSI RCATGY 3 cut(s) 1385, 1466, 2453
BstSCI CCNGG 8 cut(s) 838, 2546, 2991, 3019, 3166, 3395, 3720, 4043
BstSFI CTRYAG 3 cut(s) 231, 650, 2472
BstSLI GKGCMC 2 cut(s) 4214, 4253
BstUI CGCG 2 cut(s) 3561, 4293
BstV2I GAAGAC 2 cut(s) 1322, 1637
BstX2I RGATCY 4 cut(s) 530, 931, 1069, 1471
BstXI CCANNNNNNTGG 1 cut(s) 1613
BstYI RGATCY 4 cut(s) 530, 931, 1069, 1471
BstZ17I GTATAC 1 cut(s) 4054
Bsu15I ATCGAT 1 cut(s) 2701
BsuI GTATCC 2 cut(s) 346, 510
BsuTUI ATCGAT 1 cut(s) 2701
BtgI CCRYGG 2 cut(s) 2745, 2926
BtsCI GGATG 9 cut(s) 637, 688, 1180, 1402, 2503, 2755, 3016, 4076, 4164
BtsI GCAGTG 3 cut(s) 91, 1365, 2205
CaiI CAGNNNCTG 2 cut(s) 908, 1345
CciI TCATGA 3 cut(s) 2292, 2763, 3217
CfoI GCGC 3 cut(s) 638, 1866, 1949
Cfr10I RCCGGY 2 cut(s) 434, 2984
Cfr13I GGNCC 7 cut(s) 432, 1282, 1519, 2167, 2501, 2527, 3718
ClaI ATCGAT 1 cut(s) 2701
CseI GACGC 7 cut(s) 914, 1151, 2270, 3689, 3846, 4131, 4243
DinI GGCGCC 1 cut(s) 1948
DraIII CACNNNGTG 1 cut(s) 1488
DrdI GACNNNNNNGTC 1 cut(s) 1660
DseDI GACNNNNNNGTC 1 cut(s) 1660
EaeI YGGCCR 3 cut(s) 795, 3631, 4288
Eam1104I CTCTTC 7 cut(s) 360, 1904, 2328, 2356, 2963, 3768, 3919
EarI CTCTTC 7 cut(s) 360, 1904, 2328, 2356, 2963, 3768, 3919
Ecl136II GAGCTC 1 cut(s) 1914
Eco130I CCWWGG 6 cut(s) 270, 2170, 2926, 2937, 3956, 4179
Eco147I AGGCCT 2 cut(s) 598, 2996
Eco24I GRGCYC 2 cut(s) 1916, 3446
Eco31I GGTCTC 3 cut(s) 2296, 2781, 3980
Eco32I GATATC 1 cut(s) 135
Eco47I GGWCC 3 cut(s) 1282, 1519, 2501
Eco47III AGCGCT 1 cut(s) 1865
Eco53kI GAGCTC 1 cut(s) 1914
Eco57I CTGAAG 7 cut(s) 519, 960, 1433, 1977, 2292, 2603, 3587
EcoICRI GAGCTC 1 cut(s) 1914
EcoNI CCTNNNNNAGG 1 cut(s) 2893
EcoO109I RGGNCCY 2 cut(s) 1519, 3718
EcoRI GAATTC 2 cut(s) 2028, 4089
EcoRII CCWGG 7 cut(s) 838, 2546, 2991, 3166, 3395, 3720, 4043
EcoRV GATATC 1 cut(s) 135
EcoT14I CCWWGG 6 cut(s) 270, 2170, 2926, 2937, 3956, 4179
EcoT22I ATGCAT 3 cut(s) 467, 1464, 1975
EcoT38I GRGCYC 2 cut(s) 1916, 3446
EgeI GGCGCC 1 cut(s) 1948
EheI GGCGCC 1 cut(s) 1948
ErhI CCWWGG 6 cut(s) 270, 2170, 2926, 2937, 3956, 4179
Esp3I CGTCTC 1 cut(s) 1033
FaqI GGGAC 1 cut(s) 840
FauI CCCGC 1 cut(s) 742
FblI GTMKAC 1 cut(s) 4053
FokI GGATG 9 cut(s) 644, 695, 1187, 1389, 2510, 2762, 3003, 4083, 4151
FriOI GRGCYC 2 cut(s) 1916, 3446
GlaI GCGC 3 cut(s) 637, 1865, 1948
GsaI CCCAGC 2 cut(s) 2130, 4306
HaeII RGCGCY 3 cut(s) 639, 1867, 1950
HapII CCGG 6 cut(s) 138, 435, 1127, 2504, 2985, 3020
HgaI GACGC 7 cut(s) 914, 1151, 2270, 3689, 3846, 4131, 4243
HhaI GCGC 3 cut(s) 638, 1866, 1949
Hin1I GRCGYC 3 cut(s) 1661, 1947, 2281
Hin6I GCGC 3 cut(s) 636, 1864, 1947
HinP1I GCGC 3 cut(s) 636, 1864, 1947
HincII GTYRAC 1 cut(s) 3739
HindII GTYRAC 1 cut(s) 3739
HindIII AAGCTT 2 cut(s) 501, 1001
HpaII CCGG 6 cut(s) 138, 435, 1127, 2504, 2985, 3020
Hpy166II GTNNAC 9 cut(s) 620, 1046, 1400, 1508, 2205, 2263, 3739, 4054, 4212
Hpy8I GTNNAC 9 cut(s) 620, 1046, 1400, 1508, 2205, 2263, 3739, 4054, 4212
HpyCH4IV ACGT 6 cut(s) 1042, 1547, 1661, 1855, 2265, 3885
HpySE526I ACGT 6 cut(s) 1042, 1547, 1661, 1855, 2265, 3885
Hsp92I GRCGYC 3 cut(s) 1661, 1947, 2281
HspAI GCGC 3 cut(s) 636, 1864, 1947
KasI GGCGCC 1 cut(s) 1946
KroI GCCGGC 2 cut(s) 434, 2984
KroNI GCCGGC 2 cut(s) 436, 2986
LguI GCTCTTC 3 cut(s) 1904, 2356, 3919
LmnI GCTCC 7 cut(s) 28, 193, 484, 1160, 1180, 4030, 4309
MaeII ACGT 6 cut(s) 1042, 1547, 1661, 1855, 2265, 3885
MfeI CAATTG 2 cut(s) 1842, 2275
MflI RGATCY 4 cut(s) 530, 931, 1069, 1471
MhlI GDGCHC 6 cut(s) 409, 1916, 3446, 4214, 4253, 4314
MlsI TGGCCA 2 cut(s) 797, 3633
MluNI TGGCCA 2 cut(s) 797, 3633
Mly113I GGCGCC 1 cut(s) 1947
MlyI GAGTC 6 cut(s) 104, 269, 566, 822, 1213, 1351
MmeI TCCRAC 2 cut(s) 396, 3614
Mox20I TGGCCA 2 cut(s) 797, 3633
Mph1103I ATGCAT 3 cut(s) 467, 1464, 1975
MroNI GCCGGC 2 cut(s) 434, 2984
MroXI GAANNNNTTC 2 cut(s) 2670, 2702
MscI TGGCCA 2 cut(s) 797, 3633
MseI TTAA 7 cut(s) 720, 2234, 2538, 2570, 2603, 3474, 3938
MslI CAYNNNNRTG 8 cut(s) 318, 1958, 2210, 2258, 2333, 3981, 4069, 4271
Msp20I TGGCCA 2 cut(s) 797, 3633
MspA1I CMGCKG 4 cut(s) 335, 695, 4127, 4204
MspI CCGG 6 cut(s) 138, 435, 1127, 2504, 2985, 3020
MspR9I CCNGG 8 cut(s) 840, 2548, 2993, 3021, 3168, 3397, 3722, 4045
MunI CAATTG 2 cut(s) 1842, 2275
Mva1269I GAATGC 4 cut(s) 1595, 2148, 3949, 4195
MvaI CCWGG 7 cut(s) 840, 2548, 2993, 3168, 3397, 3722, 4045
MvnI CGCG 2 cut(s) 3561, 4293
NaeI GCCGGC 2 cut(s) 436, 2986
NarI GGCGCC 1 cut(s) 1947
NciI CCSGG 1 cut(s) 3021
NcoI CCATGG 1 cut(s) 2926
NgoMIV GCCGGC 2 cut(s) 434, 2984
NlaIV GGNNCC 8 cut(s) 168, 433, 480, 757, 1156, 1473, 1948, 3014
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 4 cut(s) 1129, 1662, 2251, 3107
NsiI ATGCAT 3 cut(s) 467, 1464, 1975
NspI RCATGY 3 cut(s) 1385, 1466, 2453
NspV TTCGAA 1 cut(s) 1789
OliI CACNNNNGTG 3 cut(s) 1958, 2258, 4271
PaeI GCATGC 1 cut(s) 1385
PagI TCATGA 3 cut(s) 2292, 2763, 3217
PasI CCCWGGG 1 cut(s) 839
PceI AGGCCT 2 cut(s) 598, 2996
PciSI GCTCTTC 3 cut(s) 1904, 2356, 3919
PcsI WCGNNNNNNNCGW 2 cut(s) 1018, 1039
PctI GAATGC 4 cut(s) 1595, 2148, 3949, 4195
PdiI GCCGGC 2 cut(s) 436, 2986
PdmI GAANNNNTTC 2 cut(s) 2670, 2702
PfeI GAWTC 9 cut(s) 95, 675, 1703, 1721, 2698, 2703, 3586, 4103, 4175
PfoI TCCNGGA 1 cut(s) 3395
PleI GAGTC 6 cut(s) 104, 268, 565, 822, 1212, 1350
PluTI GGCGCC 1 cut(s) 1950
PpsI GAGTC 6 cut(s) 104, 268, 565, 822, 1212, 1350
Ppu21I YACGTR 1 cut(s) 3886
PpuMI RGGWCCY 1 cut(s) 1519
PsiI TTATAA 5 cut(s) 459, 2952, 3126, 3509, 3696
Psp124BI GAGCTC 1 cut(s) 1916
Psp5II RGGWCCY 1 cut(s) 1519
Psp6I CCWGG 7 cut(s) 838, 2546, 2991, 3166, 3395, 3720, 4043
PspFI CCCAGC 2 cut(s) 2126, 4302
PspGI CCWGG 7 cut(s) 838, 2546, 2991, 3166, 3395, 3720, 4043
PspN4I GGNNCC 8 cut(s) 168, 433, 480, 757, 1156, 1473, 1948, 3014
PspPI GGNCC 7 cut(s) 432, 1282, 1519, 2167, 2501, 2527, 3718
PspPPI RGGWCCY 1 cut(s) 1519
PstI CTGCAG 2 cut(s) 235, 654
PstNI CAGNNNCTG 2 cut(s) 908, 1345
PsuI RGATCY 4 cut(s) 530, 931, 1069, 1471
PvuII CAGCTG 3 cut(s) 335, 695, 4127
RseI CAYNNNNRTG 8 cut(s) 318, 1958, 2210, 2258, 2333, 3981, 4069, 4271
SacI GAGCTC 1 cut(s) 1916
SapI GCTCTTC 3 cut(s) 1904, 2356, 3919
SaqAI TTAA 7 cut(s) 720, 2234, 2538, 2570, 2603, 3474, 3938
Sau96I GGNCC 7 cut(s) 432, 1282, 1519, 2167, 2501, 2527, 3718
SchI GAGTC 6 cut(s) 104, 269, 566, 822, 1213, 1351
ScrFI CCNGG 8 cut(s) 840, 2548, 2993, 3021, 3168, 3397, 3722, 4045
SduI GDGCHC 6 cut(s) 409, 1916, 3446, 4214, 4253, 4314
SfcI CTRYAG 3 cut(s) 231, 650, 2472
SfoI GGCGCC 1 cut(s) 1948
SfuI TTCGAA 1 cut(s) 1789
SinI GGWCC 3 cut(s) 1282, 1519, 2501
SmiMI CAYNNNNRTG 8 cut(s) 318, 1958, 2210, 2258, 2333, 3981, 4069, 4271
SmlI CTYRAG 6 cut(s) 240, 251, 392, 534, 830, 3900
SmoI CTYRAG 6 cut(s) 240, 251, 392, 534, 830, 3900
SpeI ACTAGT 2 cut(s) 661, 1133
SphI GCATGC 1 cut(s) 1385
SseBI AGGCCT 2 cut(s) 598, 2996
SsiI CCGC 4 cut(s) 749, 2241, 4204, 4291
SspDI GGCGCC 1 cut(s) 1946
SspI AATATT 3 cut(s) 64, 1579, 2639
SstI GAGCTC 1 cut(s) 1916
StuI AGGCCT 2 cut(s) 598, 2996
StyD4I CCNGG 8 cut(s) 838, 2546, 2991, 3019, 3166, 3395, 3720, 4043
StyI CCWWGG 6 cut(s) 270, 2170, 2926, 2937, 3956, 4179
TaiI ACGT 6 cut(s) 1045, 1550, 1664, 1858, 2268, 3888
TaqI TCGA 8 cut(s) 1033, 1053, 1207, 1789, 2701, 3373, 4113, 4318
TaqII GACCGA 1 cut(s) 3756
TatI WGTACW 4 cut(s) 1213, 1556, 1882, 2202
TauI GCSGC 1 cut(s) 4293
TfiI GAWTC 9 cut(s) 95, 675, 1703, 1721, 2698, 2703, 3586, 4103, 4175
Tru1I TTAA 7 cut(s) 720, 2234, 2538, 2570, 2603, 3474, 3938
Tru9I TTAA 7 cut(s) 720, 2234, 2538, 2570, 2603, 3474, 3938
TseFI GTSAC 4 cut(s) 1129, 1662, 2251, 3107
Tsp45I GTSAC 4 cut(s) 1129, 1662, 2251, 3107
TspGWI ACGGA 2 cut(s) 529, 3849
VneI GTGCAC 1 cut(s) 4210
VpaK11BI GGWCC 3 cut(s) 1282, 1519, 2501
XagI CCTNNNNNAGG 1 cut(s) 2893
XceI RCATGY 3 cut(s) 1385, 1466, 2453
XcmI CCANNNNNNNNNTGG 1 cut(s) 4064
XmiI GTMKAC 1 cut(s) 4053
XmnI GAANNNNTTC 2 cut(s) 2670, 2702
ZraI GACGTC 1 cut(s) 1662
Zsp2I ATGCAT 3 cut(s) 467, 1464, 1975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.