MD04G1232200.v1.1

Transcriptional regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010370
Physical Location & Seq
Forward (+)
31138868 .. 31149416
10549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1232200.v1.1.491

Sequence Viewer

Length: 4428 bp
ATGGAGGAAAACGGCGAGCAAGTGGAGGAAAGCCACGAGCAAGTGGAAAATATTGGGAGTGCTTCTAGTGGCAGTGATAGTGATTCTTTTATAGATGATTCAGAAGTTGATGAAGTATCGATATCCGGAGAAGATGAAAAATTGAAGCCAGAGGAACCATTATCTGATAAAGAAATAGAGGAGCTCATTGCTGAATTTTTGGAGGTTGAGAGTAAGGCTGCCGAGGCTCAAGAGGCACTTGAGAAAGAGTCTCTTGCCAAAGTGGAGAGTGAAGTTAGAAAGGAGTTGGCACAAACTCTTCATGGGGACAATTTGGAAGCAGCTGTTGCAGAAGAAATGGATACTTTGATAGAAGAGTGGCAAGCTGAGCTTGATGAACTTGAGACTGAGAGTGCTCATTTGTTGGAACAACTTGATGGGGCTGGCATTGAGCTACCGAGCCTTTATAAGTGCATTGAAAGTCAGGCTCCTAATGGTTGCTGCACTGAAGCTTGGAAAAGACGGATACACTGGATAGGGTCTCAAGAGACTGGAGAATTTACCAAGTCAAGGGCTGATGCGGAGCAGTATCTCCAAACCCACAGACCTGTGAGAAGGCGACACGGTAAACTATTGGAGGATGGCGCTAGTGGATTTCTGCAGAAAAAACTAGCCATAGATGGAAGCAAGGATGCTGTGACAGCTGATGTTGATTACTGGGGTTCTTTCAACAAATTATTTTCTGATGGTGCAACTGCGGGTGATGCCTTGTTTGGCAGTAAGCACTGGGCTTCTGTTTATTTGGCCAGTACGCCACAGCAAGCTGCTGAGATGGGACTTAAGTTCCCTGGGGTTGATGAGGTAGAGGAGATTGATGATATTGATGGCAATTCTGGTGATCCATTTGTTGCAGCCGCTGTTGCAAACGAAAGAGAACTGGATCTCACTGAAGAACAAAAGAAAAATTATAGGAAGGTCAAAGAAGAAGATGATGTAAATGTTGATCGGAAGCTTCAAATTCGTTTGAAACGGAGGAGACATCGAAAGAGAAGTAAACAGGATGCTGGCCGGAAAGATTTTTGCTTAGTTGATCAGGAGATAGGAAGCAACATGGACCTGTCTTCGTCTATGCTTGACTCTTCTATCAGTATCTCAAATGGGAAAATTGATCAAGATCTGGAAACCGCTAATAACATTGACCAAGAAAGCATAACGAGTAATGGTTCTTCTCCAGTGACTAGTTCCAGTGAAGCAAGAGGCTCAAAGCGTCTGAGTGAGGATGAGGAGCTAAACATTGATAATAAGAGGAGTCGAACTGTGATTATAGATAGCGATGATGACACTCCACTGAAAGATAATTCAGACTGCAATGCGATAAAATCTGAGGACCAGTCATATGTGGAAGAGAATATCTGCATAGCTGCCACCGGTGGTCTCCCTTCACAGAGTCTGAATGATAAGCTCTACTGCACTGCTTGTAGTAAGCATGCTGTTGAAGTGTGCTCACATCCACTTCTGAAGGTGATTATTTGTGCAGATTGCAGATGCTTATTGGAAAAAAGGATGCAGGTAAAGGATCCTGATTGCTCCGAGTGTTATTGTGGGTGGTGTGGACAAAGCAAGGACCTAGTAAATTGTAAATCTTGCAAGACATTGGTTTGTGCGACTTGTATAAAGAGGAATATTGGAGAGGAATGCTTATCTGATGCCCAGACCTCTGGCTGGCAATGTTGTTTCTGTTGCCCAAGTCTTCTAAAGACATTGACATCACAATTAGAGCAAGCAATAAGTTCTCAGGATTTGATAGTATCTAGCTCTGATAGTGATTCAGATAGTTCAGATTCAGAGATAGATGTTGCCATAAGGAAGTGTTCTAAGAGGAGGAGAAAGAAGAAAATTCGAAGGATCATTGATGATACTGAATTAGGAGAAGAAACCAGAAGGAAAATTGCAATTGAAAAGGAACGTCAAGAACGCTTGATGTCTTTGCAAGTACAATTTTCTGCCAAATCTAAGATGAAGAGCTTTGCAACTTGTAACGGGAGATTACCCGAAGGTGCCAGTGCTGAAGTGCTGGGTGATGCATCAGCTGGTTATATAGTGAATGTTGTGAGGGAAAAAGGTGAAGAAGCAGTTAGAATTCCTCCAAGCATCTCAGCGAAATTGAAGGCTCATCAGATCACGGGTGTGAGATTTATATGGGAGAATATCATACAGTCAGTCAGAAAAGTGAAGGCTGGGGATAAGGGTCTTGGTTGCATTCTAGCTCACATGATGGGCCTTGGTAAAACTTTCCAGGTCATAGCTTTTCTGTACACTGCAATGAGAAGTATTGATTTGGGATTAAATACTGCACTTATTGTCACCCCTGTGAATGTGCTGCACAATTGGCGTCAAGAGTTCATGAAGTGGAGGCCTTCAGAACTGAAGCCCCTTCGCATCTTCATGCTAGAAGATGTGTCAAGGGATAGAAGAGCAGAATTGCTTGCAAAGTGGAGAAGGAAGGGTGGTGTTTTTTTAATTGGCTACTCGGCTTTTAGAAACTTATCCTTTGGAAAGAATGTGAAGGATAGGCAGATAGCTACAGAAATTTGTCATGCCTTGCAGGATGGAACGGATATACTTGTTTGTGATGAGGCCCATGTTATTAAGAATACCAGGGCCGATGTAACCCAAGCCTTAAAACAAGTGAAATGCCAGAGAAGGATAGCATTAACTGGATCACCACTTCAGAACAATCTCATGGAATATTACTGTATGGTTGATTTTGTAAGGGAAGGTTTTCTTGGAAGCAGCCATGAGTTTCGGAATCGATTCCAAAATCCCATAGAGTATGGTCAACATACTAATTCAACTGTGGATGATGTGAAAATCATGAACCAACGGTCTCATATTCTTTATGAGGAGTTGAAAGGATTTGTTCAGAGAATGGACATGAATGTGGTAAAGAAGGACTTACCACCAAAAACTGTGTTTGTAATAGCTGTGAAACTTTCAACCTTACAGAGGAAATTATACAAGAGATTTCTTGTTGCCCACGGATTCACCAAGGACAAGGATTATAATGAAAAGATAGGGAAGAGAAGTTTTTTTGCCGGCTACCAAGCCTTAGCTCAGATATGGAACCATCCAGGGATCTTGCAACTAAGAAAAGATGACAAAGACTATGAGAGACGTGGAGATGCTGTTGAGAATTTTCTTGCAGATGACAGCTCTAGTGACGAAAACATTGATTATAATTTGGGTTTTGGAGAGAAAAATGTGAATGAGATTTTGCCAGGAAAAAAAGATGACATTTTTCGAAAGGATTGGTGGAATGATCTTCTTCATGAAAATGACTATAAAGAGCTTGATTACAGTGGCAAAATGGTACTCCTGCTTGACATTCTAGCTACGAGTTCTGATGTGGGGGATAAGGCATTGGTATTTAGCCAGAGCATACCAACCCTTGACCTCATCGAACTTTATCTTTCAAGGCTACCTCGACATGGGAACAAAGGGAAGTTTTGGAAGAAAGGAAAAGATTGGTACAGGCTAGATGGGAGAACAGAGGGCTCAGAAAGGCAAAAGCTGGTTGAACGCTTTAATGATCCCCTGAATAAGAGGGTGAAATGTGTTATAATTTCCACCAGAGCTGGATCGTTAGGGATAAATCTCTATGCTGCTAATCGTGTAATAATAGTTGATGGTTCTTGGAATCCAACATACGATCTTCAGGCTATATATCGTGCTTGGAGGTATGGCCAAACAAAGCCAGTGTTTGCTTACAGATTGATGGCACATGGAACTATGGAAGAAAAAATTTATAAGCGTCAGGTAACGAAGGAGGGCCTGGCTGCAAGGGTTGTTGATCGACAACAGGTACATAGGACAATATCTAAGGAAGAGATGTTGCATCTTTTTGAATTTGGTGATGATGAGAATCATGTGCTTGACCAAGATAGCGTATTCTTGAACGACAATGTGACTGGTAAAGTGGAAATTTTGCCGAAACACGTAGTGCCTCTTTCTCAAGGAAGTTGCTTTTCTGACAAGCTCATGGAAAGTTTACTTGGCAAGCATTCTCCAAGGTGGATCGCAAATTTCCACGAACATGAGACCCTTTTACAAGAGAACGAAGAAGAAAAGCTCACAAAGGAGGAGCAAGACATGGCTTGGGAAGTATACCGGAAATCATTTGGATGGGAGGAAGTGCAGAGAGTTCCTCTCAATGAATCTGCAGTCGACCAAAAACCAGCTGTATCAAATACTGCCTCATCTGCACCAACGAAGAGCATCCTTGCTGAATCCACGGCGAAGAATGCTTTTGTACAGCGGAAGTGCACTAATCTTTCGCATTTGCTGACGCTAAGAAGTCAGGGCACGAAACAGGGTTGCACCACCGTGTGTGGGGAATGTGGCCGCGAGCTGAGCTGGGAGGAGCACAGTCGAGATAGCAGGCTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000775 GO:0000785 GO:0000792 GO:0000902 GO:0000904 GO:0001674 GO:0001932 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005700 GO:0005701 GO:0005721 GO:0005722 GO:0006139 GO:0006259 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006915 GO:0006928 GO:0006935 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0008150 GO:0008152 GO:0008219 GO:0008347 GO:0009555 GO:0009605 GO:0009653 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009987 GO:0010369 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0012501 GO:0016043 GO:0016458 GO:0016462 GO:0016477 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0022008 GO:0022607 GO:0023051 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0031175 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031399 GO:0031497 GO:0032268 GO:0032501 GO:0032502 GO:0032872 GO:0032989 GO:0032990 GO:0033043 GO:0033044 GO:0034401 GO:0034622 GO:0034641 GO:0034724 GO:0034728 GO:0035556 GO:0040011 GO:0040029 GO:0042063 GO:0042221 GO:0042325 GO:0042330 GO:0042393 GO:0042585 GO:0043007 GO:0043073 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043408 GO:0043570 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046328 GO:0046483 GO:0048229 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048870 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051252 GO:0051253 GO:0051276 GO:0051674 GO:0051716 GO:0060255 GO:0061564 GO:0065003 GO:0065004 GO:0065007 GO:0070302 GO:0070828 GO:0070868 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097190 GO:0097193 GO:0097485 GO:0097549 GO:0098687 GO:0120036 GO:0120039 GO:1900049 GO:1901360 GO:1902275 GO:1902531 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1476

Amino Acids

166.64

Weight (kDa)

5.53

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 715 - 902 3.3e-07 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 718 - 1038 1.9e-53 SNF2-related domain
Helicase_C PF00271 1115 - 1241 2.1e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08600 AT1G08600 AT1G08600 AT1G08600
fragaria_vesca FvH4_6g01581
malus_domestica MD00G1149200.v1.1 MD04G1232200.v1.1
prunus_persica Prupe.6G351600_v2.0.a1 Prupe.6G351600_v2.0.a1
pyrus_communis pycom04g20560 pycom12g22990
rosa_chinensis RchiOBHm_Chr3g0449401
rosa_laevigata RLG00000025834
rosa_multiflora Rmu_ssc0000423.1_g000001
rosa_roxburghii Rroxscaffold_6G00427360
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018100 Rh3BG018400 Rh3CG017300 Rh3DG018800
rosa_wichuraiana Rw3G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 447, 3042, 3216, 3599, 3786
Acc36I ACCTGC 1 cut(s) 1537
AccB1I GGYRCC 1 cut(s) 2036
AccI GTMKAC 2 cut(s) 4143, 4203
AccII CGCG 1 cut(s) 4383
AccIII TCCGGA 1 cut(s) 125
AciI CCGC 6 cut(s) 560, 737, 894, 1164, 4294, 4381
AcoI YGGCCR 4 cut(s) 783, 1045, 3721, 4378
AcuI CTGAAG 8 cut(s) 507, 948, 1517, 2067, 2382, 2426, 2693, 3677
AcyI GRCGYC 1 cut(s) 2371
AdeI CACNNNGTG 2 cut(s) 3979, 4365
AfaI GTAC 7 cut(s) 790, 1974, 2294, 3351, 3509, 3843, 4290
AfiI CCNNNNNNNGG 5 cut(s) 549, 1701, 2985, 3467, 4368
AflII CTTAAG 1 cut(s) 818
AflIII ACRYGT 1 cut(s) 3973
AgeI ACCGGT 1 cut(s) 1406
AhlI ACTAGT 1 cut(s) 1217
AjiI CACGTC 1 cut(s) 3155
AjnI CCWGG 6 cut(s) 826, 2274, 2636, 3109, 3256, 3810
AjuI GAANNNNNNNTTGG 4 cut(s) 2748, 2780, 4014, 4046
AleI CACNNNNGTG 3 cut(s) 2165, 2348, 4361
Alw21I GWGCWC 5 cut(s) 186, 397, 1484, 4304, 4404
Alw26I GTCTC 9 cut(s) 255, 377, 521, 525, 1009, 1418, 2871, 3145, 4070
Alw44I GTGCAC 1 cut(s) 4300
AlwNI CAGNNNCTG 2 cut(s) 896, 1429
Aor13HI TCCGGA 1 cut(s) 125
AoxI GGCC 9 cut(s) 783, 1045, 2257, 2393, 2616, 2640, 3721, 3808, 4378
ApaLI GTGCAC 1 cut(s) 4300
AsiGI ACCGGT 1 cut(s) 1406
Asp700I GAANNNNTTC 2 cut(s) 2760, 2792
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 7 cut(s) 1093, 1366, 1603, 2257, 2617, 2640, 3808
AsuII TTCGAA 2 cut(s) 1879, 3280
AvaII GGWCC 3 cut(s) 1093, 1366, 1603
BaeGI GKGCMC 2 cut(s) 4304, 4343
BalI TGGCCA 2 cut(s) 785, 3723
BamHI GGATCC 1 cut(s) 1555
BanI GGYRCC 1 cut(s) 2036
BanII GRGCYC 2 cut(s) 186, 3536
BarI GAAGNNNNNNTAC 2 cut(s) 1189, 1221
BauI CACGAG 1 cut(s) 35
BbsI GAAGAC 2 cut(s) 1092, 1721
Bbv12I GWGCWC 5 cut(s) 186, 397, 1484, 4304, 4404
BceAI ACGGC 2 cut(s) 28, 4287
BciT130I CCWGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
BciVI GTATCC 2 cut(s) 334, 498
BclI TGATCA 2 cut(s) 1069, 1147
BcoDI GTCTC 9 cut(s) 255, 377, 521, 525, 1009, 1418, 2871, 3145, 4070
BcuI ACTAGT 1 cut(s) 1217
BfmI CTRYAG 3 cut(s) 638, 2562, 4197
BfoI RGCGCY 1 cut(s) 627
BfrI CTTAAG 1 cut(s) 818
BfuAI ACCTGC 1 cut(s) 1537
BfuI GTATCC 2 cut(s) 334, 498
BglII AGATCT 1 cut(s) 1153
BlpI GCTNAGC 2 cut(s) 366, 4388
Bme1390I CCNGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
Bme18I GGWCC 3 cut(s) 1093, 1366, 1603
BmgBI CACGTC 1 cut(s) 3155
BmgT120I GGNCC 7 cut(s) 1093, 1366, 1603, 2257, 2617, 2640, 3808
BmiI GGNNCC 5 cut(s) 156, 468, 1557, 2038, 3104
BmrFI CCNGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
BmrI ACTGGG 2 cut(s) 706, 775
BmuI ACTGGG 2 cut(s) 706, 775
BpiI GAAGAC 2 cut(s) 1092, 1721
BplI GAGNNNNNCTC 4 cut(s) 4168, 4200, 4170, 4202
BpmI CTGGAG 2 cut(s) 552, 1194
Bpu10I CCTNAGC 2 cut(s) 3088, 4421
Bpu1102I GCTNAGC 2 cut(s) 366, 4388
Bpu14I TTCGAA 2 cut(s) 1879, 3280
BpuEI CTTGAG 5 cut(s) 213, 260, 401, 507, 3975
Bsa29I ATCGAT 2 cut(s) 119, 2791
BsaAI YACGTR 1 cut(s) 3976
BsaBI GATNNNNATC 2 cut(s) 1152, 3900
BsaHI GRCGYC 1 cut(s) 2371
BsaI GGTCTC 4 cut(s) 525, 1418, 2871, 4070
BsaWI WCCGGW 3 cut(s) 125, 1406, 4146
BsaXI ACNNNNNCTCC 2 cut(s) 3150, 3180
Bsc4I CCNNNNNNNGG 5 cut(s) 549, 1701, 2985, 3467, 4368
Bse118I RCCGGY 2 cut(s) 1406, 3074
Bse3DI GCAATG 4 cut(s) 186, 1354, 1712, 2307
Bse8I GATNNNNATC 2 cut(s) 1152, 3900
BseAI TCCGGA 1 cut(s) 125
BseBI CCWGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
BseCI ATCGAT 2 cut(s) 119, 2791
BseJI GATNNNNATC 2 cut(s) 1152, 3900
BseLI CCNNNNNNNGG 5 cut(s) 549, 1701, 2985, 3467, 4368
BseMI GCAATG 4 cut(s) 186, 1354, 1712, 2307
BseSI GKGCMC 2 cut(s) 4304, 4343
BseYI CCCAGC 3 cut(s) 2053, 2216, 4392
BsgI GTGCAG 7 cut(s) 466, 1432, 1533, 2316, 2345, 4193, 4224
Bsh1236I CGCG 1 cut(s) 4383
BshFI GGCC 9 cut(s) 785, 1047, 2259, 2395, 2618, 2642, 3723, 3810, 4380
BshNI GGYRCC 1 cut(s) 2036
BshTI ACCGGT 1 cut(s) 1406
BshVI ATCGAT 2 cut(s) 119, 2791
BsiHKAI GWGCWC 5 cut(s) 186, 397, 1484, 4304, 4404
BsiSI CCGG 5 cut(s) 126, 1048, 1407, 3075, 4147
BslFI GGGAC 2 cut(s) 320, 828
BslI CCNNNNNNNGG 5 cut(s) 549, 1701, 2985, 3467, 4368
BsmAI GTCTC 9 cut(s) 255, 377, 521, 525, 1009, 1418, 2871, 3145, 4070
BsmBI CGTCTC 1 cut(s) 3145
BsmFI GGGAC 2 cut(s) 320, 828
BsmI GAATGC 4 cut(s) 1679, 2238, 4039, 4285
BsnI GGCC 9 cut(s) 785, 1047, 2259, 2395, 2618, 2642, 3723, 3810, 4380
Bso31I GGTCTC 4 cut(s) 525, 1418, 2871, 4070
Bsp119I TTCGAA 2 cut(s) 1879, 3280
Bsp1286I GDGCHC 7 cut(s) 186, 397, 1484, 3536, 4304, 4343, 4404
Bsp13I TCCGGA 1 cut(s) 125
Bsp1407I TGTACA 2 cut(s) 2292, 4288
Bsp1720I GCTNAGC 2 cut(s) 366, 4388
BspACI CCGC 6 cut(s) 560, 737, 894, 1164, 4294, 4381
BspANI GGCC 9 cut(s) 785, 1047, 2259, 2395, 2618, 2642, 3723, 3810, 4380
BspDI ATCGAT 2 cut(s) 119, 2791
BspEI TCCGGA 1 cut(s) 125
BspFNI CGCG 1 cut(s) 4383
BspHI TCATGA 3 cut(s) 2382, 2853, 3307
BspLI GGNNCC 5 cut(s) 156, 468, 1557, 2038, 3104
BspMAI CTGCAG 2 cut(s) 642, 4201
BspMI ACCTGC 1 cut(s) 1537
BspQI GCTCTTC 3 cut(s) 1994, 2446, 4244
BspT104I TTCGAA 2 cut(s) 1879, 3280
BspT107I GGYRCC 1 cut(s) 2036
BspTI CTTAAG 1 cut(s) 818
BspTNI GGTCTC 4 cut(s) 525, 1418, 2871, 4070
BsrDI GCAATG 4 cut(s) 186, 1354, 1712, 2307
BsrFI RCCGGY 2 cut(s) 1406, 3074
BsrGI TGTACA 2 cut(s) 2292, 4288
BssAI RCCGGY 2 cut(s) 1406, 3074
BssNAI GTATAC 1 cut(s) 4144
BssNI GRCGYC 1 cut(s) 2371
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 3 cut(s) 2260, 3027, 4046
Bst1107I GTATAC 1 cut(s) 4144
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
Bst6I CTCTTC 9 cut(s) 303, 348, 1123, 1377, 1994, 2446, 3053, 3858, 4244
BstACI GRCGYC 1 cut(s) 2371
BstAFI CTTAAG 1 cut(s) 818
BstAPI GCANNNNNTGC 1 cut(s) 326
BstAUI TGTACA 2 cut(s) 2292, 4288
BstBAI YACGTR 1 cut(s) 3976
BstBI TTCGAA 2 cut(s) 1879, 3280
BstDSI CCRYGG 2 cut(s) 3016, 4269
BstENI CCTNNNNNAGG 1 cut(s) 2983
BstFNI CGCG 1 cut(s) 4383
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 1 cut(s) 626
BstMAI GTCTC 9 cut(s) 255, 377, 521, 525, 1009, 1418, 2871, 3145, 4070
BstNI CCWGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
BstNSI RCATGY 1 cut(s) 1469
BstSCI CCNGG 6 cut(s) 826, 2274, 2636, 3109, 3256, 3810
BstSFI CTRYAG 3 cut(s) 638, 2562, 4197
BstSLI GKGCMC 2 cut(s) 4304, 4343
BstUI CGCG 1 cut(s) 4383
BstV2I GAAGAC 2 cut(s) 1092, 1721
BstX2I RGATCY 4 cut(s) 919, 1153, 1555, 3114
BstXI CCANNNNNNTGG 1 cut(s) 1697
BstYI RGATCY 4 cut(s) 919, 1153, 1555, 3114
BstZ17I GTATAC 1 cut(s) 4144
Bsu15I ATCGAT 2 cut(s) 119, 2791
BsuI GTATCC 2 cut(s) 334, 498
BsuRI GGCC 9 cut(s) 785, 1047, 2259, 2395, 2618, 2642, 3723, 3810, 4380
BsuTUI ATCGAT 2 cut(s) 119, 2791
BtgI CCRYGG 2 cut(s) 3016, 4269
BtgZI GCGATG 1 cut(s) 1326
BtrI CACGTC 1 cut(s) 3155
BtsI GCAGTG 3 cut(s) 79, 1449, 2295
BveI ACCTGC 1 cut(s) 1537
CaiI CAGNNNCTG 2 cut(s) 896, 1429
CciI TCATGA 3 cut(s) 2382, 2853, 3307
CfoI GCGC 1 cut(s) 626
Cfr10I RCCGGY 2 cut(s) 1406, 3074
Cfr13I GGNCC 7 cut(s) 1093, 1366, 1603, 2257, 2617, 2640, 3808
ClaI ATCGAT 2 cut(s) 119, 2791
CseI GACGC 4 cut(s) 1235, 2360, 3779, 4333
Csp6I GTAC 7 cut(s) 789, 1973, 2293, 3350, 3508, 3842, 4289
CspAI ACCGGT 1 cut(s) 1406
CviQI GTAC 7 cut(s) 789, 1973, 2293, 3350, 3508, 3842, 4289
DraIII CACNNNGTG 2 cut(s) 3979, 4365
EaeI YGGCCR 4 cut(s) 783, 1045, 3721, 4378
Eam1104I CTCTTC 9 cut(s) 303, 348, 1123, 1377, 1994, 2446, 3053, 3858, 4244
EarI CTCTTC 9 cut(s) 303, 348, 1123, 1377, 1994, 2446, 3053, 3858, 4244
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 3 cut(s) 2260, 3027, 4046
Eco147I AGGCCT 1 cut(s) 2395
Eco24I GRGCYC 2 cut(s) 186, 3536
Eco31I GGTCTC 4 cut(s) 525, 1418, 2871, 4070
Eco32I GATATC 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 1093, 1366, 1603
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 8 cut(s) 507, 948, 1517, 2067, 2382, 2426, 2693, 3677
EcoICRI GAGCTC 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 2983
EcoO109I RGGNCCY 2 cut(s) 1603, 3808
EcoRI GAATTC 1 cut(s) 2118
EcoRII CCWGG 6 cut(s) 826, 2274, 2636, 3109, 3256, 3810
EcoRV GATATC 1 cut(s) 123
EcoT14I CCWWGG 3 cut(s) 2260, 3027, 4046
EcoT22I ATGCAT 1 cut(s) 2065
EcoT38I GRGCYC 2 cut(s) 186, 3536
ErhI CCWWGG 3 cut(s) 2260, 3027, 4046
Esp3I CGTCTC 1 cut(s) 3145
FaqI GGGAC 2 cut(s) 320, 828
FauI CCCGC 1 cut(s) 730
FauNDI CATATG 1 cut(s) 1375
FbaI TGATCA 2 cut(s) 1069, 1147
FblI GTMKAC 2 cut(s) 4143, 4203
FriOI GRGCYC 2 cut(s) 186, 3536
GlaI GCGC 1 cut(s) 625
GsaI CCCAGC 3 cut(s) 2057, 2220, 4396
GsuI CTGGAG 2 cut(s) 552, 1194
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 9 cut(s) 785, 1047, 2259, 2395, 2618, 2642, 3723, 3810, 4380
HapII CCGG 5 cut(s) 126, 1048, 1407, 3075, 4147
HgaI GACGC 4 cut(s) 1235, 2360, 3779, 4333
HhaI GCGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 2371
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HincII GTYRAC 2 cut(s) 2819, 4204
HindII GTYRAC 2 cut(s) 2819, 4204
HindIII AAGCTT 2 cut(s) 489, 989
HpaII CCGG 5 cut(s) 126, 1048, 1407, 3075, 4147
Hpy166II GTNNAC 9 cut(s) 608, 1034, 1592, 2295, 2819, 4028, 4144, 4204, 4302
Hpy8I GTNNAC 9 cut(s) 608, 1034, 1592, 2295, 2819, 4028, 4144, 4204, 4302
HpyCH4IV ACGT 3 cut(s) 1945, 3154, 3975
HpySE526I ACGT 3 cut(s) 1945, 3154, 3975
Hsp92I GRCGYC 1 cut(s) 2371
HspAI GCGC 1 cut(s) 624
Kpn2I TCCGGA 1 cut(s) 125
KroI GCCGGC 1 cut(s) 3074
KroNI GCCGGC 1 cut(s) 3076
Ksp22I TGATCA 2 cut(s) 1069, 1147
LguI GCTCTTC 3 cut(s) 1994, 2446, 4244
LmnI GCTCC 7 cut(s) 181, 472, 562, 1264, 1571, 4120, 4399
MaeII ACGT 3 cut(s) 1945, 3154, 3975
MaeIII GTNAC 8 cut(s) 676, 1213, 2015, 2341, 2647, 3197, 3796, 3943
MfeI CAATTG 2 cut(s) 1932, 2365
MflI RGATCY 4 cut(s) 919, 1153, 1555, 3114
MhlI GDGCHC 7 cut(s) 186, 397, 1484, 3536, 4304, 4343, 4404
MlsI TGGCCA 2 cut(s) 785, 3723
MluNI TGGCCA 2 cut(s) 785, 3723
MlyI GAGTC 4 cut(s) 257, 1109, 1297, 1435
MmeI TCCRAC 2 cut(s) 384, 3704
Mox20I TGGCCA 2 cut(s) 785, 3723
Mph1103I ATGCAT 1 cut(s) 2065
MroI TCCGGA 1 cut(s) 125
MroNI GCCGGC 1 cut(s) 3074
MroXI GAANNNNTTC 2 cut(s) 2760, 2792
MscI TGGCCA 2 cut(s) 785, 3723
MseI TTAA 7 cut(s) 819, 2324, 2498, 2628, 2660, 2693, 3564
MslI CAYNNNNRTG 9 cut(s) 2165, 2300, 2348, 2423, 2918, 3312, 4071, 4159, 4361
Msp20I TGGCCA 2 cut(s) 785, 3723
MspA1I CMGCKG 6 cut(s) 323, 683, 896, 2069, 4217, 4294
MspCI CTTAAG 1 cut(s) 818
MspI CCGG 5 cut(s) 126, 1048, 1407, 3075, 4147
MspR9I CCNGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
MunI CAATTG 2 cut(s) 1932, 2365
Mva1269I GAATGC 4 cut(s) 1679, 2238, 4039, 4285
MvaI CCWGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
MvnI CGCG 1 cut(s) 4383
NaeI GCCGGC 1 cut(s) 3076
NdeI CATATG 1 cut(s) 1375
NgoMIV GCCGGC 1 cut(s) 3074
NlaIV GGNNCC 5 cut(s) 156, 468, 1557, 2038, 3104
NmeAIII GCCGAG 2 cut(s) 247, 2489
NmuCI GTSAC 5 cut(s) 676, 1213, 2341, 3197, 3943
NsiI ATGCAT 1 cut(s) 2065
NspI RCATGY 1 cut(s) 1469
NspV TTCGAA 2 cut(s) 1879, 3280
OliI CACNNNNGTG 3 cut(s) 2165, 2348, 4361
PaeI GCATGC 1 cut(s) 1469
PagI TCATGA 3 cut(s) 2382, 2853, 3307
PasI CCCWGGG 1 cut(s) 827
PceI AGGCCT 1 cut(s) 2395
PciSI GCTCTTC 3 cut(s) 1994, 2446, 4244
PctI GAATGC 4 cut(s) 1679, 2238, 4039, 4285
PdiI GCCGGC 1 cut(s) 3076
PdmI GAANNNNTTC 2 cut(s) 2760, 2792
PinAI ACCGGT 1 cut(s) 1406
PleI GAGTC 4 cut(s) 256, 1109, 1296, 1434
PpsI GAGTC 4 cut(s) 256, 1109, 1296, 1434
Ppu21I YACGTR 1 cut(s) 3976
PpuMI RGGWCCY 1 cut(s) 1603
PsiI TTATAA 5 cut(s) 447, 3042, 3216, 3599, 3786
Psp124BI GAGCTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 1603
Psp6I CCWGG 6 cut(s) 826, 2274, 2636, 3109, 3256, 3810
PspFI CCCAGC 3 cut(s) 2053, 2216, 4392
PspGI CCWGG 6 cut(s) 826, 2274, 2636, 3109, 3256, 3810
PspN4I GGNNCC 5 cut(s) 156, 468, 1557, 2038, 3104
PspPI GGNCC 7 cut(s) 1093, 1366, 1603, 2257, 2617, 2640, 3808
PspPPI RGGWCCY 1 cut(s) 1603
PstI CTGCAG 2 cut(s) 642, 4201
PstNI CAGNNNCTG 2 cut(s) 896, 1429
PsuI RGATCY 4 cut(s) 919, 1153, 1555, 3114
PvuII CAGCTG 4 cut(s) 323, 683, 2069, 4217
RsaI GTAC 7 cut(s) 790, 1974, 2294, 3351, 3509, 3843, 4290
RsaNI GTAC 7 cut(s) 789, 1973, 2293, 3350, 3508, 3842, 4289
RseI CAYNNNNRTG 9 cut(s) 2165, 2300, 2348, 2423, 2918, 3312, 4071, 4159, 4361
SacI GAGCTC 1 cut(s) 186
SalI GTCGAC 1 cut(s) 4202
SapI GCTCTTC 3 cut(s) 1994, 2446, 4244
SaqAI TTAA 7 cut(s) 819, 2324, 2498, 2628, 2660, 2693, 3564
Sau96I GGNCC 7 cut(s) 1093, 1366, 1603, 2257, 2617, 2640, 3808
SchI GAGTC 4 cut(s) 257, 1109, 1297, 1435
ScrFI CCNGG 6 cut(s) 828, 2276, 2638, 3111, 3258, 3812
SduI GDGCHC 7 cut(s) 186, 397, 1484, 3536, 4304, 4343, 4404
SfcI CTRYAG 3 cut(s) 638, 2562, 4197
SfuI TTCGAA 2 cut(s) 1879, 3280
SgrAI CRCCGGYG 1 cut(s) 1406
SinI GGWCC 3 cut(s) 1093, 1366, 1603
SmiMI CAYNNNNRTG 9 cut(s) 2165, 2300, 2348, 2423, 2918, 3312, 4071, 4159, 4361
SmlI CTYRAG 6 cut(s) 228, 239, 380, 522, 818, 3990
SmoI CTYRAG 6 cut(s) 228, 239, 380, 522, 818, 3990
SpeI ACTAGT 1 cut(s) 1217
SphI GCATGC 1 cut(s) 1469
SseBI AGGCCT 1 cut(s) 2395
SsiI CCGC 6 cut(s) 560, 737, 894, 1164, 4294, 4381
SspI AATATT 3 cut(s) 52, 1663, 2729
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 2395
StyD4I CCNGG 6 cut(s) 826, 2274, 2636, 3109, 3256, 3810
StyI CCWWGG 3 cut(s) 2260, 3027, 4046
TaiI ACGT 3 cut(s) 1948, 3157, 3978
TatI WGTACW 3 cut(s) 1972, 2292, 4288
TauI GCSGC 2 cut(s) 896, 4383
Tru1I TTAA 7 cut(s) 819, 2324, 2498, 2628, 2660, 2693, 3564
Tru9I TTAA 7 cut(s) 819, 2324, 2498, 2628, 2660, 2693, 3564
TseFI GTSAC 5 cut(s) 676, 1213, 2341, 3197, 3943
Tsp45I GTSAC 5 cut(s) 676, 1213, 2341, 3197, 3943
TspGWI ACGGA 4 cut(s) 517, 1024, 2609, 3033
Vha464I CTTAAG 1 cut(s) 818
VneI GTGCAC 1 cut(s) 4300
VpaK11BI GGWCC 3 cut(s) 1093, 1366, 1603
XagI CCTNNNNNAGG 1 cut(s) 2983
XceI RCATGY 1 cut(s) 1469
XmiI GTMKAC 2 cut(s) 4143, 4203
XmnI GAANNNNTTC 2 cut(s) 2760, 2792
Zsp2I ATGCAT 1 cut(s) 2065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.