Rh3AG018100

Transcriptional regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010370
Physical Location & Seq
Reverse (-)
1176300 .. 1189591
13292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG018100.1

Sequence Viewer

Length: 4431 bp
ATGGAAGAAAGCCATGACGTGGTGGAAGATGTGGAGAGTGGTGGTTCTAGTGGTAGTGATTTGGATTCTTTTATAGATGATTCAGAAGTTGATGAAGTATCGATATCTGGACAAGATGACGGTTTGCATCTTGAGGAACCTTTATCTGATAAAGACATTGAGGAGCTGATTGCTGAATTTTTGGAGGTTGAGAGTAAGGCTGCAGAGGCTCAGGAAGCGCTTGAAAAGGAGTCTCTTGCCAAAGTGGAGACTGAAGTTAGAGAGGAGCTGGCACAAACTCTTCACGGGAATGATTTGGAAACAACTGTTGCAGATGAAATGGCTACTTTAATAGAAGACTGGAAAGCTGAGCTCGATGAGATTGAGACTGAGAGTGCTCATTTGTTGGAACAACTTGATGGGGCAGGCATTGAGCTACCAAGCCTTTATAAGTGGATTGAAAGTCAGGCTCCTAATGGTTGCTGCACCGAAGCTTGGAAAACACGAATACACTGGGTAGGAAGTCAGGTGAGTGGAGAATTTACCGAGTCAAGAGCTGATGCTGAGAAGTATCTTCAAACGCACAGACCTGTAAGAAGGAAACATGGTAAACTATTGGAGGATGGTGCTAGTGGATTCCTGCAGAAAAAACTAGCTGAAGAGGGGAGCAAGGATGTTGCGACAACTGAGGTTGATTGGTGTTCTCTCAACAAATTATTTTCTGACGGTGCAACTAAGGATAGCGCTTCATTTGGAAGCAAGCACTGGGCTTCTGTTTACTTGGCTAGCACACCGCACCAAGCTGCTGAGATGGGACTTGAATTCCCTGGAGTCAATGAGGTGGAGGAGATTGATGACATTGATGGCAATTCTAGTGATCCATTTGTTGCAGCTGCTGTTGCAAATGAAAGAGAACTGAATCTCTCAGAAGAACAGAAAAGAAGTTATAGGAAGGTTAAGGAAGAAGATGACGCAAACATTGATAGGAAGCTTCAAGTTCATTTGAAACGGAGGAGACATCAAAGGAGATGTAAACAGGATGTCAGTCAGAAAGAAGTTTTCCCAATTGATCAAGTGATACAAAGCAACATGGGCCAGTCTTTCTCAATGCTTGACTCTTCTACTGGTATCTCAAATGGGAAAATTGATGAAGATGGTGAAAACATTTGTAACACAGATAATGAGGTTGAAGGCCAAAATTTGAAGTCTGCCATAATGAGTAACGGTGCTCCGCCGGCCCCCGATTCCACTGAAGCCAGAGGGTCCAAGCGTCCGAATGAGAGCGAGGAACTAAACATTGACATTAAGAGGAATCGAACTATAATTATAGACAGTGATGATGAAGCTTCAATGAAGGATACTTTTGACTGCAACATGATAAAATCTGAGGATCATTCCTATGTGAAAGAGAATATCTGCAGATCTGGAGATGATGGTCTCACATCACATAGTCTGAATAAGATGCTTCGTTGCACAGCTTGTAATAAATTTTCTGCTGAAGTGCGCTCACATCCACTTATGGAAGTGATTATTTGTGCAAATTGCAAGTGCTTATTGGAAGAAATAATGCATTTCAAGGATTCTGATTGCTCCATGTGTTATTGTGGGTGGTGTGGGCAAAGCAATGATTTATTGAGTTGTAAATCTTGCACGATGTTATTTTGTACAACTTGTATAAAGAGGAATATTGGAGAGGAATGCTTATCCAAGGCCCAGACCGATGGCTGGCAGTGTTGTTCCTGTTGCCCAAGTCTCCTACAGACATTGAAGTTACAATTTGAGGAAGCAATGGGATCTGGAGATTTGATAGTTTCTAGCTCTGATAGTGATTCAGATGATTCAGATGCAGATGTGGATGTTGCTATCAGTTCTAAGAGGAAGAGAAAGAAGAAGATACGAAGGATAATTGATGATACCGAATTGGGAGAAGAGACCAAAAGAAAAATTGCGATTGAAAAGGAACGTCAAGAGCGTTTGAAGTCCTTGCAAGTACAATTTTCTGTCAAATCTAAAATGAAGTGCTCTGCTAGCTATAATGGGAATATATCTGAAGGTGTTAGTGCTGAAGTCATGGGTGATGCATCAGCTGGTTATATAGTGAATGTTATGAGAGAGAAAGGTGAAGAACCTGTGAGAATTCCTCCAAGCCTTTCATCAAAATTGAAGGCTCATCAGATTATGGGCGTAAGGTTTATGTGGGAGAACATCGTACAATCTATCAGAGAAGTGAAGTCTGGGGATAAAGGCCTTGGTTGTATTCTAGCTCACATGATGGGCCTTGGTAAAACGCTTCAGGTTATTATTTTTCTGTATACTGCAATGAGAAGTATCGATTTGGGATTAAAAACTGCACTTATTGTCACTCCTGTTAATGTGCTGCATAATTGGCGGCAAGAGTTCTTGAAGTGGAGACCTTCAGAAGTAAAGCCTCTTCGGATCTTCATGCTAGAAGATGTGTCAAGGGAGAGGAGACTAGAGGTGCTTGCAAAATGGAGAGCTAAGGGTGGTGTTTTCTTGATTGGCTACTCCGCTTTCAGAAACTTATCCCTTGGAAAGCATGTGAAGGATCGGAACATGGCTAGAGAAATTTGTCATGCCTTACAGGATGGACCTGATATACTTGTTTGTGATGAGGCCCATATTATTAAGAATACCAAGGCCGATGTAACCCAGGCCTTGAAACAAGTTAGATGCCAAAGAAGAATAGCATTAACTGGATCACCTCTTCAGAACAATCTCATGGAATATTATTGTATGGTTGATTTTGTAAGGGAAGGTTTTCTTGGAAGTAGCCACGAGTTTAGAAACCGCTTCCAGAATCCCATAGAGAATGGTCAGCATACTAATTCAACTGTGGAAGATGTGAAAATCATGAATCAGAGATCGCATATTCTGTATGAACAGTTGAAAGGATTTGTTCAAAGAATGGACATGAGCGTGGTGAAGAAGGACTTACCACCTAAAACTGTCTTTGTGATAACTGTGAGGCTTTCTCCCATACAGAAGAAATTATACAAGAGATTCCTTGATGTGCACGGATTCACTACTGACAAGATTTATAATGAAAAGATGAAAAGAGGTTTTTTCGCTGGCTACCAGGCCTTAGCTCAGATATGGAACCATCCAGGGATATTGCAATTGAGAAAAGATGACAAAGACTATGTGAGGCATGAAGATGGTGTTGAGAATTTTCTTGCTGATGACAGCTCTAGTGATGAAAACACTGACTATATAGGAGAGAAGCAAGGGAATGTCAATGAAATTTTGCCAGGAAAAAAAGATGATGGTTACTTTCAGAAGGATTGGTGGAATGATCTCATCCATGAAAATAATTATAAGGAGCTTGATTATAGTGGCAAAATGGTATTGCTGCTTGAAATTCTATCTATGTGTTCTGATGTGGGTGATAAGGCATTGGTATTTAGTCAGAGCATACCAACTCTTGATCTGATAGAACTTTACCTTGCAAGATTACCTCGACATGGGAAGAGATGGAAGTTTTGGAAGAAAGGAAAAGATTGGTTTAGGCTAGATGGTAGAACAGAGAGCTCAGAAAGGCAAAAACTGGTTGAGCGCTTTAATGATCCTGCAAATAAGAGGGTGAAATGCACTCTAATTTCCACCAAAGCTGGATCTTTGGGGATAAATCTTCATGCTGCTAACCGTGTAATCATAGTTGATGGGTCTTGGAATCCAACATATGATCTTCAGGCTATCTATCGTGCTTGGAGGTATGGCCAAACAAAGCCGGTGTTTGCTTACAGATTGATGGCACATGGAACTATGGAAGAAAAAATTTATAAGCGTCAGGTAACAAAGGAGGGCCTGGCTGCAAGGGTAGTGGATAGACAACAGGTACATAGGACAATATCTAAAGAAGAGATGTTACATCTTTTTGAGTTTGGTGACGATGAAAACCATGATGGGCCTGAGCAAGATAACAGAGCTAATCAAAGTATCACTGGTTCAGTCGAAAATTTGCCTAAGCACGGACCACATCTTTCTCATGGAAGTTGCGCTTCTGACAAATTAATGGAAAGATTAATTGGCAAGCATTATCCGAGTTGGATTGCTAATTTTCACTTACATGAGACACTATTGCAAGAGAATGAAGAGGAAAAGCTATCAAAAGAAGAGCAAGACATGGCTTGGGAAGTATACCGGAAATCATTTGAATGGGAGGAAGTGAAGCAAGTTCCTGTCAGTGAATCTGCAGTTGACCAAAAGCCAGAGCCAGCTGCATCAACTGTGAACTCGCCTGCAATAGAGATGAGCAGCCTTGCAGAATCCAAGGTGAGGGATACTTATGTGCAGCGGAAATGCACTAAGCTTTCACATGTGCTGACCCTTAGAAGTCAAGGTACAAAATCTGGTTGCACCACTGTGTGTGGGGAATGTGCTCGAGAGATAAGCTGGGAGGGTTTGCATCAAGAAGGCAAGTCTGCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000775 GO:0000785 GO:0000792 GO:0000902 GO:0000904 GO:0001674 GO:0001932 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005700 GO:0005701 GO:0005721 GO:0005722 GO:0006139 GO:0006259 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006915 GO:0006928 GO:0006935 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0008150 GO:0008152 GO:0008219 GO:0008347 GO:0009555 GO:0009605 GO:0009653 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009987 GO:0010369 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0012501 GO:0016043 GO:0016458 GO:0016462 GO:0016477 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0022008 GO:0022607 GO:0023051 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0031175 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031399 GO:0031497 GO:0032268 GO:0032501 GO:0032502 GO:0032872 GO:0032989 GO:0032990 GO:0033043 GO:0033044 GO:0034401 GO:0034622 GO:0034641 GO:0034724 GO:0034728 GO:0035556 GO:0040011 GO:0040029 GO:0042063 GO:0042221 GO:0042325 GO:0042330 GO:0042393 GO:0042585 GO:0043007 GO:0043073 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043408 GO:0043570 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046328 GO:0046483 GO:0048229 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048870 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051252 GO:0051253 GO:0051276 GO:0051674 GO:0051716 GO:0060255 GO:0061564 GO:0065003 GO:0065004 GO:0065007 GO:0070302 GO:0070828 GO:0070868 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097190 GO:0097193 GO:0097485 GO:0097549 GO:0098687 GO:0120036 GO:0120039 GO:1900049 GO:1901360 GO:1902275 GO:1902531 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1476

Amino Acids

167.02

Weight (kDa)

5.49

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 717 - 1036 1.4e-52 SNF2-related domain
ResIII PF04851 726 - 901 2.8e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 1113 - 1239 5.2e-19 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08600 AT1G08600 AT1G08600 AT1G08600
fragaria_vesca FvH4_6g01581
malus_domestica MD00G1149200.v1.1 MD04G1232200.v1.1
prunus_persica Prupe.6G351600_v2.0.a1 Prupe.6G351600_v2.0.a1
pyrus_communis pycom04g20560 pycom12g22990
rosa_chinensis RchiOBHm_Chr3g0449401
rosa_laevigata RLG00000025834
rosa_multiflora Rmu_ssc0000423.1_g000001
rosa_roxburghii Rroxscaffold_6G00427360
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018100 Rh3BG018400 Rh3CG017300 Rh3DG018800
rosa_wichuraiana Rw3G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 429, 3039, 3315, 3780
AccB7I CCANNNNNTGG 1 cut(s) 19
AccI GTMKAC 2 cut(s) 2291, 4137
AciI CCGC 6 cut(s) 773, 1211, 2368, 2508, 2788, 4294
AclWI GGATC 8 cut(s) 851, 1377, 1780, 2423, 2553, 2704, 3557, 3619
AcoI YGGCCR 1 cut(s) 3715
AdeI CACNNNGTG 1 cut(s) 4365
AfaI GTAC 5 cut(s) 1645, 1971, 2190, 3837, 4342
AfeI AGCGCT 3 cut(s) 219, 724, 3554
AfiI CCNNNNNNNGG 6 cut(s) 19, 474, 1704, 3461, 3968, 4275
AflIII ACRYGT 1 cut(s) 4315
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 6 cut(s) 805, 2649, 3075, 3103, 3247, 3804
AjuI GAANNNNNNNTTGG 4 cut(s) 2745, 2777, 4008, 4040
AleI CACNNNNGTG 1 cut(s) 4361
Alw21I GWGCWC 7 cut(s) 354, 379, 1210, 2003, 3015, 3530, 4381
Alw44I GTGCAC 1 cut(s) 3011
AlwI GGATC 8 cut(s) 851, 1377, 1780, 2423, 2553, 2704, 3557, 3619
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 1 cut(s) 4380
Aor51HI AGCGCT 3 cut(s) 219, 724, 3554
ApaLI GTGCAC 1 cut(s) 3011
AseI ATTAAT 2 cut(s) 4010, 4022
Asp700I GAANNNNTTC 1 cut(s) 2757
AspLEI GCGC 5 cut(s) 220, 725, 1485, 3555, 3998
AsuNHI GCTAGC 2 cut(s) 764, 2006
AvaI CYCGRG 1 cut(s) 4380
AvaII GGWCC 3 cut(s) 1242, 2588, 3971
BaeGI GKGCMC 1 cut(s) 3015
BaeI ACNNNNGTAYC 2 cut(s) 4272, 4305
BalI TGGCCA 1 cut(s) 3717
BanII GRGCYC 2 cut(s) 354, 3530
BarI GAAGNNNNNNTAC 8 cut(s) 2394, 2426, 2975, 3007, 3849, 3881, 4324, 4356
BauI CACGAG 1 cut(s) 2774
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 7 cut(s) 354, 379, 1210, 2003, 3015, 3530, 4381
BciT130I CCWGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
BciVI GTATCC 2 cut(s) 1330, 4273
BclI TGATCA 1 cut(s) 1048
BfmI CTRYAG 5 cut(s) 201, 620, 1396, 1736, 4191
BfoI RGCGCY 3 cut(s) 221, 726, 3556
BfuI GTATCC 2 cut(s) 1330, 4273
BglII AGATCT 1 cut(s) 1400
BlpI GCTNAGC 1 cut(s) 348
Bme1390I CCNGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
Bme18I GGWCC 3 cut(s) 1242, 2588, 3971
BmeT110I CYCGRG 1 cut(s) 4380
BmgBI CACGTC 1 cut(s) 19
BmiI GGNNCC 5 cut(s) 138, 450, 1218, 1243, 3098
BmrFI CCNGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
BmrI ACTGGG 2 cut(s) 502, 754
BmsI GCATC 9 cut(s) 136, 529, 1431, 1813, 2047, 2069, 2660, 4229, 4414
BmtI GCTAGC 2 cut(s) 768, 2010
BmuI ACTGGG 2 cut(s) 502, 754
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 4 cut(s) 2104, 2136, 2956, 2988
BpmI CTGGAG 3 cut(s) 828, 1425, 1797
Bpu10I CCTNAGC 5 cut(s) 210, 2478, 3082, 3909, 3963
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 152
Bsa29I ATCGAT 2 cut(s) 101, 2310
BsaI GGTCTC 3 cut(s) 1421, 1904, 2383
BsaJI CCNNGG 9 cut(s) 805, 1686, 2227, 2257, 2527, 2634, 2649, 3104, 4269
BsaWI WCCGGW 1 cut(s) 4140
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 474, 1704, 3461, 3968, 4275
Bse118I RCCGGY 2 cut(s) 1213, 3727
Bse1I ACTGG 8 cut(s) 344, 497, 749, 1075, 1108, 2698, 3549, 3946
Bse3DI GCAATG 3 cut(s) 1609, 1773, 2304
BseBI CCWGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
BseCI ATCGAT 2 cut(s) 101, 2310
BseDI CCNNGG 9 cut(s) 805, 1686, 2227, 2257, 2527, 2634, 2649, 3104, 4269
BseGI GGATG 8 cut(s) 607, 658, 1024, 1489, 1840, 2590, 3100, 3297
BseLI CCNNNNNNNGG 6 cut(s) 19, 474, 1704, 3461, 3968, 4275
BseMI GCAATG 3 cut(s) 1609, 1773, 2304
BseNI ACTGG 8 cut(s) 344, 497, 749, 1075, 1108, 2698, 3549, 3946
BseRI GAGGAG 5 cut(s) 176, 278, 839, 1006, 2461
BseSI GKGCMC 1 cut(s) 3015
BseYI CCCAGC 1 cut(s) 4392
BsgI GTGCAG 3 cut(s) 448, 2313, 4310
BshVI ATCGAT 2 cut(s) 101, 2310
BsiHKAI GWGCWC 7 cut(s) 354, 379, 1210, 2003, 3015, 3530, 4381
BsiHKCI CYCGRG 1 cut(s) 4380
BsiSI CCGG 3 cut(s) 1214, 3728, 4141
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 6 cut(s) 19, 474, 1704, 3461, 3968, 4275
BsmFI GGGAC 1 cut(s) 807
BsmI GAATGC 1 cut(s) 1682
Bso31I GGTCTC 3 cut(s) 1421, 1904, 2383
BsoBI CYCGRG 1 cut(s) 4380
Bsp1286I GDGCHC 7 cut(s) 354, 379, 1210, 2003, 3015, 3530, 4381
Bsp1407I TGTACA 1 cut(s) 1643
Bsp1720I GCTNAGC 1 cut(s) 348
BspACI CCGC 6 cut(s) 773, 1211, 2368, 2508, 2788, 4294
BspDI ATCGAT 2 cut(s) 101, 2310
BspHI TCATGA 1 cut(s) 2850
BspLI GGNNCC 5 cut(s) 138, 450, 1218, 1243, 3098
BspMAI CTGCAG 4 cut(s) 205, 624, 1400, 4195
BspOI GCTAGC 2 cut(s) 768, 2010
BspPI GGATC 8 cut(s) 851, 1377, 1780, 2423, 2553, 2704, 3557, 3619
BspQI GCTCTTC 1 cut(s) 4107
BspTNI GGTCTC 3 cut(s) 1421, 1904, 2383
BsrDI GCAATG 3 cut(s) 1609, 1773, 2304
BsrFI RCCGGY 2 cut(s) 1213, 3727
BsrGI TGTACA 1 cut(s) 1643
BsrI ACTGG 8 cut(s) 344, 497, 749, 1075, 1108, 2698, 3549, 3946
BssAI RCCGGY 2 cut(s) 1213, 3727
BssECI CCNNGG 9 cut(s) 805, 1686, 2227, 2257, 2527, 2634, 2649, 3104, 4269
BssNAI GTATAC 2 cut(s) 2292, 4138
BssSI CACGAG 1 cut(s) 2774
BssT1I CCWWGG 6 cut(s) 1686, 2227, 2257, 2527, 2634, 4269
Bst1107I GTATAC 2 cut(s) 2292, 4138
Bst2BI CACGAG 1 cut(s) 2774
Bst2UI CCWGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
BstAUI TGTACA 1 cut(s) 1643
BstF5I GGATG 8 cut(s) 607, 658, 1024, 1489, 1840, 2590, 3100, 3297
BstH2I RGCGCY 3 cut(s) 221, 726, 3556
BstHHI GCGC 5 cut(s) 220, 725, 1485, 3555, 3998
BstMWI GCNNNNNNNGC 7 cut(s) 206, 215, 878, 1071, 1214, 2007, 2365
BstNI CCWGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
BstNSI RCATGY 2 cut(s) 2540, 4319
BstSCI CCNGG 6 cut(s) 805, 2649, 3075, 3103, 3247, 3804
BstSFI CTRYAG 5 cut(s) 201, 620, 1396, 1736, 4191
BstSLI GKGCMC 1 cut(s) 3015
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 4 cut(s) 1400, 1772, 2415, 3611
BstXI CCANNNNNNTGG 1 cut(s) 1700
BstYI RGATCY 4 cut(s) 1400, 1772, 2415, 3611
BstZ17I GTATAC 2 cut(s) 2292, 4138
Bsu15I ATCGAT 2 cut(s) 101, 2310
BsuI GTATCC 2 cut(s) 1330, 4273
BsuTUI ATCGAT 2 cut(s) 101, 2310
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 8 cut(s) 607, 658, 1024, 1489, 1840, 2590, 3100, 3297
BtsI GCAGTG 1 cut(s) 1715
BtsIMutI CAGTG 9 cut(s) 490, 742, 1227, 1318, 1715, 3201, 3939, 4189, 4359
CaiI CAGNNNCTG 1 cut(s) 875
CciI TCATGA 1 cut(s) 2850
CfoI GCGC 5 cut(s) 220, 725, 1485, 3555, 3998
Cfr10I RCCGGY 2 cut(s) 1213, 3727
ClaI ATCGAT 2 cut(s) 101, 2310
CseI GACGC 3 cut(s) 959, 1238, 3773
Csp6I GTAC 5 cut(s) 1644, 1970, 2189, 3836, 4341
CspCI CAANNNNNGTGG 2 cut(s) 3801, 3836
CviQI GTAC 5 cut(s) 1644, 1970, 2189, 3836, 4341
DraIII CACNNNGTG 1 cut(s) 4365
EaeI YGGCCR 1 cut(s) 3715
EciI GGCGGA 1 cut(s) 1200
Ecl136II GAGCTC 2 cut(s) 352, 3528
Eco130I CCWWGG 6 cut(s) 1686, 2227, 2257, 2527, 2634, 4269
Eco147I AGGCCT 3 cut(s) 2226, 2654, 3080
Eco24I GRGCYC 2 cut(s) 354, 3530
Eco31I GGTCTC 3 cut(s) 1421, 1904, 2383
Eco32I GATATC 1 cut(s) 105
Eco47I GGWCC 3 cut(s) 1242, 2588, 3971
Eco47III AGCGCT 3 cut(s) 219, 724, 3554
Eco53kI GAGCTC 2 cut(s) 352, 3528
Eco88I CYCGRG 1 cut(s) 4380
EcoICRI GAGCTC 2 cut(s) 352, 3528
EcoO109I RGGNCCY 1 cut(s) 3802
EcoRI GAATTC 2 cut(s) 800, 2115
EcoRII CCWGG 6 cut(s) 805, 2649, 3075, 3103, 3247, 3804
EcoRV GATATC 1 cut(s) 105
EcoT14I CCWWGG 6 cut(s) 1686, 2227, 2257, 2527, 2634, 4269
EcoT22I ATGCAT 2 cut(s) 1551, 2062
EcoT38I GRGCYC 2 cut(s) 354, 3530
ErhI CCWWGG 6 cut(s) 1686, 2227, 2257, 2527, 2634, 4269
FalI AAGNNNNNCTT 6 cut(s) 2745, 2777, 2915, 2947, 3982, 4014
FaqI GGGAC 1 cut(s) 807
FauNDI CATATG 1 cut(s) 3679
FbaI TGATCA 1 cut(s) 1048
FblI GTMKAC 2 cut(s) 2291, 4137
FokI GGATG 8 cut(s) 614, 665, 1031, 1476, 1847, 2597, 3087, 3284
FriOI GRGCYC 2 cut(s) 354, 3530
GlaI GCGC 5 cut(s) 219, 724, 1484, 3554, 3997
GsaI CCCAGC 1 cut(s) 4396
GsuI CTGGAG 3 cut(s) 828, 1425, 1797
HaeII RGCGCY 3 cut(s) 221, 726, 3556
HapII CCGG 3 cut(s) 1214, 3728, 4141
HgaI GACGC 3 cut(s) 959, 1238, 3773
HhaI GCGC 5 cut(s) 220, 725, 1485, 3555, 3998
Hin6I GCGC 5 cut(s) 218, 723, 1483, 3553, 3996
HinP1I GCGC 5 cut(s) 218, 723, 1483, 3553, 3996
HincII GTYRAC 1 cut(s) 4198
HindII GTYRAC 1 cut(s) 4198
HindIII AAGCTT 4 cut(s) 471, 968, 1323, 4307
HpaII CCGG 3 cut(s) 1214, 3728, 4141
Hpy166II GTNNAC 8 cut(s) 590, 757, 1013, 2292, 3013, 4138, 4198, 4231
Hpy8I GTNNAC 8 cut(s) 590, 757, 1013, 2292, 3013, 4138, 4198, 4231
HpyCH4IV ACGT 2 cut(s) 18, 1942
HpyF10VI GCNNNNNNNGC 7 cut(s) 206, 215, 878, 1071, 1214, 2007, 2365
HpySE526I ACGT 2 cut(s) 18, 1942
HspAI GCGC 5 cut(s) 218, 723, 1483, 3553, 3996
KroI GCCGGC 1 cut(s) 1213
KroNI GCCGGC 1 cut(s) 1215
Ksp22I TGATCA 1 cut(s) 1048
LguI GCTCTTC 1 cut(s) 4107
LmnI GCTCC 7 cut(s) 163, 265, 454, 645, 1213, 1574, 3319
LweI GCATC 9 cut(s) 136, 529, 1431, 1813, 2047, 2069, 2660, 4229, 4414
MaeII ACGT 2 cut(s) 18, 1942
MaeIII GTNAC 9 cut(s) 1148, 1199, 1749, 2338, 2644, 3266, 3790, 3864, 3884
MfeI CAATTG 2 cut(s) 1044, 3116
MflI RGATCY 4 cut(s) 1400, 1772, 2415, 3611
MhlI GDGCHC 7 cut(s) 354, 379, 1210, 2003, 3015, 3530, 4381
MlsI TGGCCA 1 cut(s) 3717
MluNI TGGCCA 1 cut(s) 3717
MlyI GAGTC 4 cut(s) 239, 536, 819, 1088
MmeI TCCRAC 3 cut(s) 366, 3698, 4025
Mox20I TGGCCA 1 cut(s) 3717
Mph1103I ATGCAT 2 cut(s) 1551, 2062
MroNI GCCGGC 1 cut(s) 1213
MroXI GAANNNNTTC 1 cut(s) 2757
MscI TGGCCA 1 cut(s) 3717
MslI CAYNNNNRTG 7 cut(s) 288, 1377, 2915, 3153, 4065, 4153, 4361
Msp20I TGGCCA 1 cut(s) 3717
MspA1I CMGCKG 4 cut(s) 872, 2066, 4217, 4294
MspI CCGG 3 cut(s) 1214, 3728, 4141
MspR9I CCNGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
MunI CAATTG 2 cut(s) 1044, 3116
Mva1269I GAATGC 1 cut(s) 1682
MvaI CCWGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
MwoI GCNNNNNNNGC 7 cut(s) 206, 215, 878, 1071, 1214, 2007, 2365
NaeI GCCGGC 1 cut(s) 1215
NdeI CATATG 1 cut(s) 3679
NgoMIV GCCGGC 1 cut(s) 1213
NheI GCTAGC 2 cut(s) 764, 2006
NlaIV GGNNCC 5 cut(s) 138, 450, 1218, 1243, 3098
NmuCI GTSAC 2 cut(s) 2338, 3884
NsiI ATGCAT 2 cut(s) 1551, 2062
NspI RCATGY 2 cut(s) 2540, 4319
OliI CACNNNNGTG 1 cut(s) 4361
PaeR7I CTCGAG 1 cut(s) 4380
PagI TCATGA 1 cut(s) 2850
PceI AGGCCT 3 cut(s) 2226, 2654, 3080
PciI ACATGT 1 cut(s) 4315
PciSI GCTCTTC 1 cut(s) 4107
PcsI WCGNNNNNNNCGW 1 cut(s) 1948
PctI GAATGC 1 cut(s) 1682
PdiI GCCGGC 1 cut(s) 1215
PdmI GAANNNNTTC 1 cut(s) 2757
PflMI CCANNNNNTGG 1 cut(s) 19
PleI GAGTC 4 cut(s) 238, 535, 818, 1088
PpsI GAGTC 4 cut(s) 238, 535, 818, 1088
PscI ACATGT 1 cut(s) 4315
PshBI ATTAAT 2 cut(s) 4010, 4022
PsiI TTATAA 4 cut(s) 429, 3039, 3315, 3780
Psp124BI GAGCTC 2 cut(s) 354, 3530
Psp6I CCWGG 6 cut(s) 805, 2649, 3075, 3103, 3247, 3804
PspFI CCCAGC 1 cut(s) 4392
PspGI CCWGG 6 cut(s) 805, 2649, 3075, 3103, 3247, 3804
PspN4I GGNNCC 5 cut(s) 138, 450, 1218, 1243, 3098
PsrI GAACNNNNNNTAC 2 cut(s) 3928, 3960
PstI CTGCAG 4 cut(s) 205, 624, 1400, 4195
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 4 cut(s) 1400, 1772, 2415, 3611
PvuII CAGCTG 3 cut(s) 872, 2066, 4217
RsaI GTAC 5 cut(s) 1645, 1971, 2190, 3837, 4342
RsaNI GTAC 5 cut(s) 1644, 1970, 2189, 3836, 4341
RseI CAYNNNNRTG 7 cut(s) 288, 1377, 2915, 3153, 4065, 4153, 4361
SacI GAGCTC 2 cut(s) 354, 3530
SapI GCTCTTC 1 cut(s) 4107
SchI GAGTC 4 cut(s) 239, 536, 819, 1088
ScrFI CCNGG 6 cut(s) 807, 2651, 3077, 3105, 3249, 3806
SduI GDGCHC 7 cut(s) 354, 379, 1210, 2003, 3015, 3530, 4381
SfaNI GCATC 9 cut(s) 136, 529, 1431, 1813, 2047, 2069, 2660, 4229, 4414
SfcI CTRYAG 5 cut(s) 201, 620, 1396, 1736, 4191
Sfr274I CTCGAG 1 cut(s) 4380
SinI GGWCC 3 cut(s) 1242, 2588, 3971
SlaI CTCGAG 1 cut(s) 4380
SmiMI CAYNNNNRTG 7 cut(s) 288, 1377, 2915, 3153, 4065, 4153, 4361
SmlI CTYRAG 2 cut(s) 131, 4380
SmoI CTYRAG 2 cut(s) 131, 4380
SseBI AGGCCT 3 cut(s) 2226, 2654, 3080
SsiI CCGC 6 cut(s) 773, 1211, 2368, 2508, 2788, 4294
SspI AATATT 2 cut(s) 1666, 2726
SstI GAGCTC 2 cut(s) 354, 3530
StuI AGGCCT 3 cut(s) 2226, 2654, 3080
StyD4I CCNGG 6 cut(s) 805, 2649, 3075, 3103, 3247, 3804
StyI CCWWGG 6 cut(s) 1686, 2227, 2257, 2527, 2634, 4269
TaiI ACGT 2 cut(s) 21, 1945
TaqI TCGA 7 cut(s) 101, 354, 1294, 2310, 3457, 3951, 4381
TaqII GACCGA 1 cut(s) 1712
TatI WGTACW 2 cut(s) 1643, 1969
TauI GCSGC 1 cut(s) 2371
TscAI CASTG 9 cut(s) 497, 749, 1234, 1318, 1715, 3208, 3946, 4189, 4366
TseFI GTSAC 2 cut(s) 2338, 3884
Tsp45I GTSAC 2 cut(s) 2338, 3884
TspGWI ACGGA 3 cut(s) 1003, 3030, 3984
TspRI CASTG 9 cut(s) 497, 749, 1234, 1318, 1715, 3208, 3946, 4189, 4366
Van91I CCANNNNNTGG 1 cut(s) 19
VneI GTGCAC 1 cut(s) 3011
VpaK11BI GGWCC 3 cut(s) 1242, 2588, 3971
VspI ATTAAT 2 cut(s) 4010, 4022
XceI RCATGY 2 cut(s) 2540, 4319
XhoI CTCGAG 1 cut(s) 4380
XmiI GTMKAC 2 cut(s) 2291, 4137
XmnI GAANNNNTTC 1 cut(s) 2757
Zsp2I ATGCAT 2 cut(s) 1551, 2062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.