pycom04g20560

Transcriptional regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010370
Physical Location & Seq
Forward (+)
22029484 .. 22035096
5613 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20560.13

Sequence Viewer

Length: 2754 bp
ATGGACCTGTCTTCGTCTATGCTTGACTCTTCTATTAGTATCTCAAATGGGAAAATTGATGAAGATCTGGAAACCGCTAATAACATTGACCAAGAAAGCATAATGAGTAACGGTGCTTCTCCGGTGACTAGTTCCAGTGAAGCAAGAGGCTCTAAGCGTCTGAGTAAGGATGAGGAGCTAAACATTGATAATAAGAGGAGTCGAACTGTGATTATAGATAGCGATGATGACACTCCACTGAAAGATAATTCAGACTGCAATGCGATAAAATCTGAGGACCAGTCATATGTGGAAGAGAATATCTGCATAGCTGCCACCGGTGGTCTCCCTTCACAGAGTCTGAATGATAAGCTCTACTGCACTGCTTGTAGTAAGCATGCTGTTGAAGTGTGCTCACATCCACTTCTGAAGGTGATTATTTGTGCGGATTGCAGATGCTTATTGGAAAAAAAGATGCAGGTGAAGGATCCTGATTGCTCCGAGTGTTATTGTGGGTGGTGTGGACAAAGCAAGGACCTAGTAAATTGTAAATCTTGCAAGACATTGGTTTGTGCGACTTGTATAAAGAGGAATATTGGAGAGGAATGCTTATCTGATGCCCAGACCTCTGGCTGGCAATGTTGTTTCTGTTGCCCAAGTCTTCTAAAGACATTGACCTCACAATTAGAGCAAGCAATAAGTTCTCAGGATTTGATAGTATCTAGCTCTGATAGTGATTCAGATAGTTCAGATTCAGAGATAGATGTTGCCATAAGTTCTAAGAGGAGGAGAAAGAAGAAAATTCGAAGGATCATTGATGATACTGAATTAGGAGAAGAAACCAGAAGGAAAATTGCAATTGAAAAGGAACGTCAAGAACGCTTGATGTCTTTGCAAGTACAATTTTCTGCCAAATCTAAGATGAAGAGCTTTGCAACTTGTAATGGGAGATTACCCGAAGGCGCCAGCGCGGAAGTGCTGGGTGATGCATCAGCTGGTTATATAGTGAATGTTGTGAGGGAAAAAGGTGAAGAAGCTGTTAGAATTCCTCCAAGCATCTCAGCGAAATTGAAGGCTCATCAGATCACGGGTGTGAGATTTATATGGGAGAATATCATACAGTCAGTCAGAAAAGTGAAGGCTGGGGATAAGGGTCTTGGTTGCATTCTAGCTCACATGATGGGCCTTGGTAAAACTTTCCAGGTCATAGCTTTTCTGTACACTGCAATGAGAAGTATTGATTTGGGATTAAATACTGCACTTATTGTCACCCCTGTGAATGTGCTGCACAATTGGCGTCAAGAGTTCATGAAGTGGAGGCCTTCAGAACTGAAGCCCCTTCGCATCTTCATGCTAGAAGATGTGTCAAGGGATAGAAGAGCAGAATTGCTTGCAAAGTGGAGAAGGAAGGGTGGTGTTTTTTTAATTGGCTACTCGGCTTTTAGAAACTTATCCTTTGGAAAGAATGTGAAGGATAGGCAGATAGCTACAGAAATTTGTCATGCCTTGCAGGATGGAACGGATATACTTGTTTGTGATGAGGCCCATGTTATTAAGAATACCAGGGCTGATGTAACCCATGCCTTAAAACAAGTGAAATGCCAGAGAAGGATAGCATTAACTGGGTCACCACTTCAGAACAATCTCATGGAATATTACTGTATGGTTGATTTTGTAAGGGAAGGTTTTCTTGGAAGCAGCCATGAGTTTCGGAATCGATTTCAAAATCCCATAGAGTATGGTCAACATACTAATTCAACTGTGGATGATGTGAAAATCATGAACCAACGGTCTCATATTCTTTATGAAGAGTTGAAAGGATTTGTTCAGAGAATGGACATGAATGTGGTGAAGAAGGACTTACCACCAAAAACCGTGTTTGTAATAGCTGTGAAACTTTCAACCTTACAGAGGAAATTATACAAGAGATTTCTTGTTGCCCACGGATTCACCAAGGACAAGGATTATAATGAAAAGATAGGGAAGAGAAGTTTTTTTGCTGGCTACCAAGCCTTAGCTCAGATATGGAACCATCCTGGGATCTTGCAACTAAGAAAAGATGACAAAGACTATGAGAGACGTGGAGATGCCATTGAGAATTTTCTTGCAGATGACAGCTCTAGTGACGAAAACATTGATTATAATTTGGGTTTTGGAGAGAAAAATGTGAATGAAATTTTGCCAGGAAAAAAAGATGACATTTTTCGAAAGGATTGGTGGAATGATCTTCTCCATGAAAATGACTATAAAGAGCTTGATTACAGTGGTAAAATGGTACTGCTGCTTGACATTCTAGCTATGAGTTCTGATGTGGGGGATAAGGCATTGGTATTTAGCCAGAGCATACCAACCCTTGACCTCATCGAACTTTATCTTTCAAGGCTACCTCGACATGGGAACAAAGGGAAGTTTTGGAAGAAAGGAAAAGATTGGTACAGGCTAGATGGGAGAACAGAGGGCTCAGAAAGGCAAAGACTGGTGGAACGATTTAATGATCCCCTGAATAAGAGGGTGAAATGTGTTATAATCTCCACCAGAGCTGGATCGTTAGGGATAAATCTCTATGCTGCTAATCGTGTAATAATAGTTGATGGTTCTTGGAATCCAACATCCGATCTTCAGGCTATATATCGTGCTTGGAGGTACGGCCAAACAAAGCCAGTGTTCGCTTACAGATTGATGGCACATGGAACTATGGAAGAAAAAATTTATAAGCGTCAGGTAACGAAGGAGGGCCTGGCTGCAAGGGTTGATCGATCGACAACAGGTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000775 GO:0000785 GO:0000792 GO:0000902 GO:0000904 GO:0001674 GO:0001932 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005700 GO:0005701 GO:0005721 GO:0005722 GO:0006139 GO:0006259 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006915 GO:0006928 GO:0006935 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0008150 GO:0008152 GO:0008219 GO:0008347 GO:0009555 GO:0009605 GO:0009653 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009987 GO:0010369 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0012501 GO:0016043 GO:0016458 GO:0016462 GO:0016477 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0022008 GO:0022607 GO:0023051 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0031175 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031399 GO:0031497 GO:0032268 GO:0032501 GO:0032502 GO:0032872 GO:0032989 GO:0032990 GO:0033043 GO:0033044 GO:0034401 GO:0034622 GO:0034641 GO:0034724 GO:0034728 GO:0035556 GO:0040011 GO:0040029 GO:0042063 GO:0042221 GO:0042325 GO:0042330 GO:0042393 GO:0042585 GO:0043007 GO:0043073 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043408 GO:0043570 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046328 GO:0046483 GO:0048229 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048870 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051252 GO:0051253 GO:0051276 GO:0051674 GO:0051716 GO:0060255 GO:0061564 GO:0065003 GO:0065004 GO:0065007 GO:0070302 GO:0070828 GO:0070868 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097190 GO:0097193 GO:0097485 GO:0097549 GO:0098687 GO:0120036 GO:0120039 GO:1900049 GO:1901360 GO:1902275 GO:1902531 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

918

Amino Acids

103.7

Weight (kDa)

8.57

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08600 AT1G08600 AT1G08600 AT1G08600
fragaria_vesca FvH4_6g01581
malus_domestica MD00G1149200.v1.1 MD04G1232200.v1.1
prunus_persica Prupe.6G351600_v2.0.a1 Prupe.6G351600_v2.0.a1
pyrus_communis pycom04g20560 pycom12g22990
rosa_chinensis RchiOBHm_Chr3g0449401
rosa_laevigata RLG00000025834
rosa_multiflora Rmu_ssc0000423.1_g000001
rosa_roxburghii Rroxscaffold_6G00427360
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018100 Rh3BG018400 Rh3CG017300 Rh3DG018800
rosa_wichuraiana Rw3G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1947, 2121, 2504, 2691
AarI CACCTGC 1 cut(s) 448
Acc36I ACCTGC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 941
AccII CGCG 1 cut(s) 950
AciI CCGC 3 cut(s) 75, 425, 950
AclWI GGATC 6 cut(s) 461, 474, 797, 2027, 2468, 2530
AcoI YGGCCR 1 cut(s) 2626
AcsI RAATTY 6 cut(s) 780, 1023, 1473, 2077, 2154, 2685
AcuI CTGAAG 5 cut(s) 428, 1287, 1331, 1598, 2582
AcyI GRCGYC 2 cut(s) 942, 1276
AfaI GTAC 6 cut(s) 879, 1199, 2256, 2414, 2624, 2749
AfiI CCNNNNNNNGG 3 cut(s) 612, 1890, 2372
AgeI ACCGGT 1 cut(s) 317
AgsI TTSAA 8 cut(s) 386, 842, 1051, 1703, 1737, 1795, 1881, 2358
AhlI ACTAGT 1 cut(s) 128
AjiI CACGTC 1 cut(s) 2060
AjnI CCWGG 5 cut(s) 1179, 1541, 2014, 2161, 2715
AjuI GAANNNNNNNTTGG 2 cut(s) 1653, 1685
AleI CACNNNNGTG 2 cut(s) 1070, 1253
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 3 cut(s) 329, 1776, 2050
AlwI GGATC 6 cut(s) 461, 474, 797, 2027, 2468, 2530
AlwNI CAGNNNCTG 1 cut(s) 340
AoxI GGCC 5 cut(s) 1162, 1298, 1521, 2626, 2713
ApeKI GCWGC 6 cut(s) 311, 1264, 1677, 2260, 2546, 2720
ApoI RAATTY 6 cut(s) 780, 1023, 1473, 2077, 2154, 2685
AsiGI ACCGGT 1 cut(s) 317
Asp700I GAANNNNTTC 1 cut(s) 1665
AspLEI GCGC 2 cut(s) 944, 950
AspS9I GGNCC 6 cut(s) 4, 277, 514, 1162, 1522, 2713
AsuII TTCGAA 2 cut(s) 784, 2185
AvaII GGWCC 3 cut(s) 4, 277, 514
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 941
BanII GRGCYC 1 cut(s) 2441
BarI GAAGNNNNNNTAC 2 cut(s) 100, 132
BbsI GAAGAC 2 cut(s) 3, 632
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 6 cut(s) 298, 1251, 1689, 2247, 2533, 2707
BccI CCATC 6 cut(s) 1153, 1487, 2019, 2417, 2564, 2653
BceAI ACGGC 1 cut(s) 2641
BciT130I CCWGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
BcoDI GTCTC 3 cut(s) 329, 1776, 2050
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 8 cut(s) 129, 518, 702, 1148, 1334, 2100, 2273, 2420
BfmI CTRYAG 1 cut(s) 1467
BfoI RGCGCY 1 cut(s) 945
BfuAI ACCTGC 1 cut(s) 448
BglII AGATCT 1 cut(s) 64
BisI GCNGC 6 cut(s) 312, 1265, 1678, 2261, 2547, 2721
BlsI GCNGC 6 cut(s) 313, 1266, 1679, 2262, 2548, 2722
Bme1390I CCNGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
Bme18I GGWCC 3 cut(s) 4, 277, 514
BmgBI CACGTC 1 cut(s) 2060
BmgT120I GGNCC 6 cut(s) 4, 277, 514, 1162, 1522, 2713
BmiI GGNNCC 3 cut(s) 468, 943, 2009
BmrFI CCNGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
BmrI ACTGGG 1 cut(s) 1611
BmsI GCATC 8 cut(s) 425, 444, 586, 955, 977, 1044, 1332, 2056
BmuI ACTGGG 1 cut(s) 1611
BpiI GAAGAC 2 cut(s) 3, 632
Bpu10I CCTNAGC 1 cut(s) 1993
Bpu14I TTCGAA 2 cut(s) 784, 2185
Bsa29I ATCGAT 2 cut(s) 1696, 2734
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 2 cut(s) 942, 1276
BsaI GGTCTC 2 cut(s) 329, 1776
BsaJI CCNNGG 5 cut(s) 1165, 1542, 1921, 1932, 2015
BsaWI WCCGGW 2 cut(s) 121, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 612, 1890, 2372
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 5 cut(s) 135, 280, 1606, 2460, 2639
Bse3DI GCAATG 3 cut(s) 265, 623, 1212
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
BseCI ATCGAT 2 cut(s) 1696, 2734
BseDI CCNNGG 5 cut(s) 1165, 1542, 1921, 1932, 2015
BseGI GGATG 6 cut(s) 175, 397, 1498, 1750, 2011, 2588
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 3 cut(s) 612, 1890, 2372
BseMI GCAATG 3 cut(s) 265, 623, 1212
BseMII CTCAG 6 cut(s) 152, 264, 698, 1053, 2012, 2454
BseNI ACTGG 5 cut(s) 135, 280, 1606, 2460, 2639
BseRI GAGGAG 4 cut(s) 188, 211, 778, 781
BseXI GCAGC 6 cut(s) 298, 1251, 1689, 2247, 2533, 2707
BseYI CCCAGC 2 cut(s) 958, 1121
BsgI GTGCAG 3 cut(s) 343, 1221, 1250
Bsh1236I CGCG 1 cut(s) 950
Bsh1285I CGRYCG 1 cut(s) 2738
BshFI GGCC 5 cut(s) 1164, 1300, 1523, 2628, 2715
BshNI GGYRCC 1 cut(s) 941
BshTI ACCGGT 1 cut(s) 317
BshVI ATCGAT 2 cut(s) 1696, 2734
BsiEI CGRYCG 1 cut(s) 2738
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 2 cut(s) 122, 318
BslI CCNNNNNNNGG 3 cut(s) 612, 1890, 2372
BsmAI GTCTC 3 cut(s) 329, 1776, 2050
BsmBI CGTCTC 1 cut(s) 2050
BsmI GAATGC 2 cut(s) 590, 1143
BsnI GGCC 5 cut(s) 1164, 1300, 1523, 2628, 2715
Bso31I GGTCTC 2 cut(s) 329, 1776
Bsp119I TTCGAA 2 cut(s) 784, 2185
Bsp1286I GDGCHC 2 cut(s) 395, 2441
Bsp1407I TGTACA 1 cut(s) 1197
BspACI CCGC 3 cut(s) 75, 425, 950
BspANI GGCC 5 cut(s) 1164, 1300, 1523, 2628, 2715
BspCNI CTCAG 6 cut(s) 153, 265, 697, 1052, 2011, 2453
BspDI ATCGAT 2 cut(s) 1696, 2734
BspFNI CGCG 1 cut(s) 950
BspHI TCATGA 2 cut(s) 1287, 1758
BspLI GGNNCC 3 cut(s) 468, 943, 2009
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 6 cut(s) 461, 474, 797, 2027, 2468, 2530
BspQI GCTCTTC 2 cut(s) 899, 1351
BspT104I TTCGAA 2 cut(s) 784, 2185
BspT107I GGYRCC 1 cut(s) 941
BspTNI GGTCTC 2 cut(s) 329, 1776
BsrDI GCAATG 3 cut(s) 265, 623, 1212
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 1197
BsrI ACTGG 5 cut(s) 135, 280, 1606, 2460, 2639
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 5 cut(s) 1165, 1542, 1921, 1932, 2015
BssNI GRCGYC 2 cut(s) 942, 1276
BssT1I CCWWGG 2 cut(s) 1165, 1932
Bst2UI CCWGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
Bst4CI ACNGT 8 cut(s) 113, 208, 1101, 1640, 1741, 1770, 1855, 2243
Bst6I CTCTTC 6 cut(s) 34, 288, 899, 1351, 1782, 1958
BstACI GRCGYC 2 cut(s) 942, 1276
BstAUI TGTACA 1 cut(s) 1197
BstBI TTCGAA 2 cut(s) 784, 2185
BstC8I GCNNGC 6 cut(s) 378, 614, 672, 946, 1371, 1981
BstDSI CCRYGG 1 cut(s) 1921
BstEII GGTNACC 1 cut(s) 1605
BstENI CCTNNNNNAGG 1 cut(s) 1888
BstF5I GGATG 6 cut(s) 175, 397, 1498, 1750, 2011, 2588
BstFNI CGCG 1 cut(s) 950
BstH2I RGCGCY 1 cut(s) 945
BstHHI GCGC 2 cut(s) 944, 950
BstMAI GTCTC 3 cut(s) 329, 1776, 2050
BstMCI CGRYCG 1 cut(s) 2738
BstMWI GCNNNNNNNGC 1 cut(s) 1273
BstNI CCWGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
BstNSI RCATGY 1 cut(s) 380
BstPI GGTNACC 1 cut(s) 1605
BstSCI CCNGG 5 cut(s) 1179, 1541, 2014, 2161, 2715
BstSFI CTRYAG 1 cut(s) 1467
BstUI CGCG 1 cut(s) 950
BstV1I GCAGC 6 cut(s) 298, 1251, 1689, 2247, 2533, 2707
BstV2I GAAGAC 2 cut(s) 3, 632
BstX2I RGATCY 3 cut(s) 64, 466, 2019
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 3 cut(s) 64, 466, 2019
Bsu15I ATCGAT 2 cut(s) 1696, 2734
BsuRI GGCC 5 cut(s) 1164, 1300, 1523, 2628, 2715
BsuTUI ATCGAT 2 cut(s) 1696, 2734
BtgI CCRYGG 1 cut(s) 1921
BtgZI GCGATG 1 cut(s) 237
BtrI CACGTC 1 cut(s) 2060
BtsCI GGATG 6 cut(s) 175, 397, 1498, 1750, 2011, 2588
BtsI GCAGTG 2 cut(s) 360, 1200
BtsIMutI CAGTG 6 cut(s) 142, 236, 360, 1200, 2248, 2646
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 6 cut(s) 378, 614, 672, 946, 1371, 1981
CaiI CAGNNNCTG 1 cut(s) 340
CciI TCATGA 2 cut(s) 1287, 1758
CfoI GCGC 2 cut(s) 944, 950
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 6 cut(s) 4, 277, 514, 1162, 1522, 2713
ClaI ATCGAT 2 cut(s) 1696, 2734
CseI GACGC 3 cut(s) 146, 1265, 2684
Csp6I GTAC 6 cut(s) 878, 1198, 2255, 2413, 2623, 2748
CspAI ACCGGT 1 cut(s) 317
CviQI GTAC 6 cut(s) 878, 1198, 2255, 2413, 2623, 2748
DinI GGCGCC 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 2626
Eam1104I CTCTTC 6 cut(s) 34, 288, 899, 1351, 1782, 1958
EarI CTCTTC 6 cut(s) 34, 288, 899, 1351, 1782, 1958
Eco130I CCWWGG 2 cut(s) 1165, 1932
Eco147I AGGCCT 1 cut(s) 1300
Eco24I GRGCYC 1 cut(s) 2441
Eco31I GGTCTC 2 cut(s) 329, 1776
Eco47I GGWCC 3 cut(s) 4, 277, 514
Eco57I CTGAAG 5 cut(s) 428, 1287, 1331, 1598, 2582
Eco91I GGTNACC 1 cut(s) 1605
EcoNI CCTNNNNNAGG 1 cut(s) 1888
EcoO109I RGGNCCY 2 cut(s) 514, 2713
EcoO65I GGTNACC 1 cut(s) 1605
EcoRI GAATTC 1 cut(s) 1023
EcoRII CCWGG 5 cut(s) 1179, 1541, 2014, 2161, 2715
EcoT14I CCWWGG 2 cut(s) 1165, 1932
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 1 cut(s) 2441
EgeI GGCGCC 1 cut(s) 943
EheI GGCGCC 1 cut(s) 943
ErhI CCWWGG 2 cut(s) 1165, 1932
Esp3I CGTCTC 1 cut(s) 2050
FalI AAGNNNNNCTT 4 cut(s) 1653, 1685, 1823, 1855
FauNDI CATATG 1 cut(s) 286
Fnu4HI GCNGC 6 cut(s) 312, 1265, 1678, 2261, 2547, 2721
FokI GGATG 6 cut(s) 182, 384, 1505, 1757, 1998, 2575
FriOI GRGCYC 1 cut(s) 2441
Fsp4HI GCNGC 6 cut(s) 312, 1265, 1678, 2261, 2547, 2721
FspBI CTAG 8 cut(s) 129, 518, 702, 1148, 1334, 2100, 2273, 2420
GlaI GCGC 2 cut(s) 943, 949
GluI GCNGC 6 cut(s) 312, 1265, 1678, 2261, 2547, 2721
GsaI CCCAGC 2 cut(s) 962, 1125
HaeII RGCGCY 1 cut(s) 945
HaeIII GGCC 5 cut(s) 1164, 1300, 1523, 2628, 2715
HapII CCGG 2 cut(s) 122, 318
HgaI GACGC 3 cut(s) 146, 1265, 2684
HhaI GCGC 2 cut(s) 944, 950
Hin1I GRCGYC 2 cut(s) 942, 1276
Hin6I GCGC 2 cut(s) 942, 948
HinP1I GCGC 2 cut(s) 942, 948
HincII GTYRAC 1 cut(s) 1724
HindII GTYRAC 1 cut(s) 1724
HinfI GANTC 8 cut(s) 26, 199, 337, 716, 731, 1693, 1926, 2581
HpaII CCGG 2 cut(s) 122, 318
Hpy166II GTNNAC 3 cut(s) 503, 1200, 1724
Hpy188III TCNNGA 7 cut(s) 68, 470, 686, 854, 1280, 1288, 1759
Hpy8I GTNNAC 3 cut(s) 503, 1200, 1724
HpyCH4III ACNGT 8 cut(s) 113, 208, 1101, 1640, 1741, 1770, 1855, 2243
HpyCH4IV ACGT 2 cut(s) 850, 2059
HpyF10VI GCNNNNNNNGC 1 cut(s) 1273
HpySE526I ACGT 2 cut(s) 850, 2059
Hsp92I GRCGYC 2 cut(s) 942, 1276
HspAI GCGC 2 cut(s) 942, 948
KasI GGCGCC 1 cut(s) 941
LguI GCTCTTC 2 cut(s) 899, 1351
LmnI GCTCC 2 cut(s) 175, 482
Lsp1109I GCAGC 6 cut(s) 298, 1251, 1689, 2247, 2533, 2707
LweI GCATC 8 cut(s) 425, 444, 586, 955, 977, 1044, 1332, 2056
MaeI CTAG 8 cut(s) 129, 518, 702, 1148, 1334, 2100, 2273, 2420
MaeII ACGT 2 cut(s) 850, 2059
MaeIII GTNAC 7 cut(s) 107, 124, 1246, 1552, 1605, 2102, 2701
MfeI CAATTG 2 cut(s) 837, 1270
MflI RGATCY 3 cut(s) 64, 466, 2019
MhlI GDGCHC 2 cut(s) 395, 2441
Mly113I GGCGCC 1 cut(s) 942
MlyI GAGTC 3 cut(s) 20, 208, 346
MmeI TCCRAC 1 cut(s) 2609
Mph1103I ATGCAT 1 cut(s) 970
MroXI GAANNNNTTC 1 cut(s) 1665
MseI TTAA 6 cut(s) 1229, 1403, 1533, 1565, 1598, 2469
MslI CAYNNNNRTG 6 cut(s) 1070, 1205, 1253, 1328, 1823, 2217
MspA1I CMGCKG 1 cut(s) 974
MspI CCGG 2 cut(s) 122, 318
MspR9I CCNGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
MunI CAATTG 2 cut(s) 837, 1270
Mva1269I GAATGC 2 cut(s) 590, 1143
MvaI CCWGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
MvnI CGCG 1 cut(s) 950
MwoI GCNNNNNNNGC 1 cut(s) 1273
NarI GGCGCC 1 cut(s) 942
NdeI CATATG 1 cut(s) 286
NlaIV GGNNCC 3 cut(s) 468, 943, 2009
NmeAIII GCCGAG 1 cut(s) 1394
NmuCI GTSAC 4 cut(s) 124, 1246, 1605, 2102
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 1 cut(s) 380
NspV TTCGAA 2 cut(s) 784, 2185
OliI CACNNNNGTG 2 cut(s) 1070, 1253
PaeI GCATGC 1 cut(s) 380
PagI TCATGA 2 cut(s) 1287, 1758
PaqCI CACCTGC 1 cut(s) 448
PceI AGGCCT 1 cut(s) 1300
PciSI GCTCTTC 2 cut(s) 899, 1351
PctI GAATGC 2 cut(s) 590, 1143
PdmI GAANNNNTTC 1 cut(s) 1665
PfeI GAWTC 5 cut(s) 716, 731, 1693, 1926, 2581
PinAI ACCGGT 1 cut(s) 317
PkrI GCNGC 6 cut(s) 313, 1266, 1679, 2262, 2548, 2722
Ple19I CGATCG 1 cut(s) 2738
PleI GAGTC 3 cut(s) 20, 207, 345
PluTI GGCGCC 1 cut(s) 945
PpsI GAGTC 3 cut(s) 20, 207, 345
PpuMI RGGWCCY 1 cut(s) 514
PsiI TTATAA 4 cut(s) 1947, 2121, 2504, 2691
Psp5II RGGWCCY 1 cut(s) 514
Psp6I CCWGG 5 cut(s) 1179, 1541, 2014, 2161, 2715
PspEI GGTNACC 1 cut(s) 1605
PspFI CCCAGC 2 cut(s) 958, 1121
PspGI CCWGG 5 cut(s) 1179, 1541, 2014, 2161, 2715
PspN4I GGNNCC 3 cut(s) 468, 943, 2009
PspPI GGNCC 6 cut(s) 4, 277, 514, 1162, 1522, 2713
PspPPI RGGWCCY 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 340
PsuI RGATCY 3 cut(s) 64, 466, 2019
PvuI CGATCG 1 cut(s) 2738
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 6 cut(s) 879, 1199, 2256, 2414, 2624, 2749
RsaNI GTAC 6 cut(s) 878, 1198, 2255, 2413, 2623, 2748
RseI CAYNNNNRTG 6 cut(s) 1070, 1205, 1253, 1328, 1823, 2217
SapI GCTCTTC 2 cut(s) 899, 1351
SaqAI TTAA 6 cut(s) 1229, 1403, 1533, 1565, 1598, 2469
SatI GCNGC 6 cut(s) 312, 1265, 1678, 2261, 2547, 2721
Sau96I GGNCC 6 cut(s) 4, 277, 514, 1162, 1522, 2713
SchI GAGTC 3 cut(s) 20, 208, 346
ScrFI CCNGG 5 cut(s) 1181, 1543, 2016, 2163, 2717
SduI GDGCHC 2 cut(s) 395, 2441
SfaNI GCATC 8 cut(s) 425, 444, 586, 955, 977, 1044, 1332, 2056
SfcI CTRYAG 1 cut(s) 1467
SfoI GGCGCC 1 cut(s) 943
SfuI TTCGAA 2 cut(s) 784, 2185
SgrAI CRCCGGYG 1 cut(s) 317
SinI GGWCC 3 cut(s) 4, 277, 514
SmiMI CAYNNNNRTG 6 cut(s) 1070, 1205, 1253, 1328, 1823, 2217
SpeI ACTAGT 1 cut(s) 128
SphI GCATGC 1 cut(s) 380
SseBI AGGCCT 1 cut(s) 1300
SsiI CCGC 3 cut(s) 75, 425, 950
SspDI GGCGCC 1 cut(s) 941
SspI AATATT 2 cut(s) 574, 1634
SspMI CTAG 8 cut(s) 129, 518, 702, 1148, 1334, 2100, 2273, 2420
StuI AGGCCT 1 cut(s) 1300
StyD4I CCNGG 5 cut(s) 1179, 1541, 2014, 2161, 2715
StyI CCWWGG 2 cut(s) 1165, 1932
TaaI ACNGT 8 cut(s) 113, 208, 1101, 1640, 1741, 1770, 1855, 2243
TaiI ACGT 2 cut(s) 853, 2062
TaqI TCGA 8 cut(s) 202, 784, 1696, 2185, 2343, 2368, 2734, 2738
TatI WGTACW 2 cut(s) 877, 1197
TfiI GAWTC 5 cut(s) 716, 731, 1693, 1926, 2581
Tru1I TTAA 6 cut(s) 1229, 1403, 1533, 1565, 1598, 2469
Tru9I TTAA 6 cut(s) 1229, 1403, 1533, 1565, 1598, 2469
TscAI CASTG 6 cut(s) 142, 243, 367, 1207, 2248, 2646
TseFI GTSAC 4 cut(s) 124, 1246, 1605, 2102
TseI GCWGC 6 cut(s) 311, 1264, 1677, 2260, 2546, 2720
Tsp45I GTSAC 4 cut(s) 124, 1246, 1605, 2102
TspGWI ACGGA 2 cut(s) 1514, 1938
TspRI CASTG 6 cut(s) 142, 243, 367, 1207, 2248, 2646
VpaK11BI GGWCC 3 cut(s) 4, 277, 514
XagI CCTNNNNNAGG 1 cut(s) 1888
XapI RAATTY 6 cut(s) 780, 1023, 1473, 2077, 2154, 2685
XceI RCATGY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 1665
XspI CTAG 8 cut(s) 129, 518, 702, 1148, 1334, 2100, 2273, 2420
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.