MD00G1183300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0018489
Physical Location & Seq
Reverse (-)
43298140 .. 43298412
273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1183300.v1.1.491

Sequence Viewer

Length: 273 bp
ATGTTAGAAGGGGAACAGGTGCACAAGAGGGTTCTGGTAAGTGGGTTTGGGGGTGGTGTCGAGTATGCACGCCTGGTGTTTGATGGTATGCGTGAGAGGAGTGTTGCAAGTTGTAATTCGCTGCTTGCGGGCTATGTATGGTGTCGGGATGTTGATGGGGCGCAGAGGATTTTTAACGAGATATCGAAGTGGAATGTTGTTTCGTGGACCACCCTGATTGTGGGGTGTGCTCAGAATGGGAGGTATAAGCAAGCTTTGTCTTTGTTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

91

Amino Acids

10.0

Weight (kDa)

8.57

Isoelectric Point (pI)

27.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 220
AjnI CCWGG 1 cut(s) 72
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
Alw21I GWGCWC 2 cut(s) 24, 232
Alw44I GTGCAC 1 cut(s) 20
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 207
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 24, 232
BbvI GCAGC 1 cut(s) 108
BccI CCATC 2 cut(s) 77, 149
BciT130I CCWGG 1 cut(s) 74
BisI GCNGC 1 cut(s) 122
BlsI GCNGC 1 cut(s) 123
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 74
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 24, 232
BslI CCNNNNNNNGG 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 24, 232
BspACI CCGC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 70, 126, 130, 252
BstDEI CTNAG 1 cut(s) 231
BstF5I GGATG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 163
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 108
BtsCI GGATG 1 cut(s) 154
Cac8I GCNNGC 4 cut(s) 70, 126, 130, 252
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 207
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviJI RGCY 2 cut(s) 132, 254
CviKI_1 RGCY 2 cut(s) 132, 254
DdeI CTNAG 1 cut(s) 231
Eco32I GATATC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 72
EcoRV GATATC 1 cut(s) 183
FaiI YATR 5 cut(s) 66, 89, 135, 139, 246
FauI CCCGC 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 122
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 1 cut(s) 122
GlaI GCGC 1 cut(s) 162
GluI GCNGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 163
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HindIII AAGCTT 1 cut(s) 252
Hpy166II GTNNAC 2 cut(s) 22, 207
Hpy188I TCNGA 1 cut(s) 234
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 2 cut(s) 22, 207
HpyCH4V TGCA 3 cut(s) 22, 68, 107
HpyF3I CTNAG 1 cut(s) 231
HspAI GCGC 1 cut(s) 161
LpnPI CCDG 5 cut(s) 2, 20, 59, 86, 227
Lsp1109I GCAGC 1 cut(s) 108
MhlI GDGCHC 2 cut(s) 24, 232
MluCI AATT 1 cut(s) 115
MnlI CCTC 4 cut(s) 21, 90, 159, 234
MseI TTAA 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 74
MvaI CCWGG 1 cut(s) 74
PkrI GCNGC 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspPI GGNCC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 122
Sau96I GGNCC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 2 cut(s) 24, 232
SetI ASST 3 cut(s) 21, 245, 256
SinI GGWCC 1 cut(s) 207
Sse9I AATT 1 cut(s) 115
SsiI CCGC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 72
TaqI TCGA 2 cut(s) 60, 185
TasI AATT 1 cut(s) 115
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TseI GCWGC 1 cut(s) 121
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 207
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.