MD05G1258100.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018489
Physical Location & Seq
Reverse (-)
39429361 .. 39429767
407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1258100.v1.1.491

Sequence Viewer

Length: 354 bp
ATGATCAGAGGCCACGCTTGGAGCGAAACTCCGTGCAAGTCAGTTGAATTGTATAAACGGTTGGTGCGTTTGGAGGCAGAGGCTGATGGGTTTTCCTATTCGTATGTTCTTAGTGCTTGTGCGAGGTCAGGGTTGGTGATAGAAGGGGAACAGGTGCACAAGAGGGTTCTGGTAAGTGGGAGTATTGCAAGTTGGAATTCGCTGCTTGCGGGCTATGTATGGTGTCAGGATGTTGATGGGGCGCAGAGGATTTTTAACGAGATAACGAAGTGGAATGTTGTTTCGTGGACCACCATGATTGTGGGGTGTGCTCAGAATGGGAGGTATAAGCAAGCTTTGTCTTTGTTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

118

Amino Acids

13.06

Weight (kDa)

8.4

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 94 - 116 8.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 209
AcsI RAATTY 1 cut(s) 196
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
Alw21I GWGCWC 2 cut(s) 159, 313
Alw44I GTGCAC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 83
AoxI GGCC 1 cut(s) 10
ApaLI GTGCAC 1 cut(s) 155
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 196
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 288
BaeGI GKGCMC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 159, 313
BbvI GCAGC 1 cut(s) 189
BccI CCATC 2 cut(s) 80, 230
BclI TGATCA 1 cut(s) 3
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BseGI GGATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 159, 313
BsnI GGCC 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 159, 313
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 209
BspANI GGCC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 325
BssMI GATC 1 cut(s) 3
Bst4CI ACNGT 1 cut(s) 60
BstC8I GCNNGC 3 cut(s) 207, 211, 333
BstDEI CTNAG 2 cut(s) 110, 312
BstF5I GGATG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstSLI GKGCMC 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 189
BstXI CCANNNNNNTGG 1 cut(s) 301
BsuRI GGCC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 207, 211, 333
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 288
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviAII CATG 1 cut(s) 295
CviJI RGCY 4 cut(s) 12, 83, 213, 335
CviKI_1 RGCY 4 cut(s) 12, 83, 213, 335
DdeI CTNAG 2 cut(s) 110, 312
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 196
FaeI CATG 1 cut(s) 298
FaiI YATR 6 cut(s) 54, 105, 216, 220, 296, 327
FatI CATG 1 cut(s) 294
FauI CCCGC 1 cut(s) 202
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 1 cut(s) 203
GlaI GCGC 1 cut(s) 243
GluI GCNGC 1 cut(s) 203
HaeIII GGCC 1 cut(s) 12
HhaI GCGC 1 cut(s) 244
Hin1II CATG 1 cut(s) 298
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HindIII AAGCTT 1 cut(s) 333
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 157, 288
Hpy188I TCNGA 2 cut(s) 8, 315
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 2 cut(s) 157, 288
HpyAV CCTTC 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4V TGCA 3 cut(s) 36, 157, 188
HpyF3I CTNAG 2 cut(s) 110, 312
Hsp92II CATG 1 cut(s) 298
HspAI GCGC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 21
LpnPI CCDG 4 cut(s) 114, 137, 155, 212
Lsp1109I GCAGC 1 cut(s) 189
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MhlI GDGCHC 2 cut(s) 159, 313
MluCI AATT 2 cut(s) 47, 196
MmeI TCCRAC 1 cut(s) 173
MnlI CCTC 6 cut(s) 67, 73, 117, 156, 240, 315
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 299
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 298
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 64
PkrI GCNGC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 1 cut(s) 288
SduI GDGCHC 2 cut(s) 159, 313
SetI ASST 4 cut(s) 128, 156, 326, 337
SinI GGWCC 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 2 cut(s) 47, 196
SsiI CCGC 1 cut(s) 209
TaaI ACNGT 1 cut(s) 60
TasI AATT 2 cut(s) 47, 196
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseI GCWGC 1 cut(s) 202
TspGWI ACGGA 1 cut(s) 21
VneI GTGCAC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.