MD00G1220200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0018025
Physical Location & Seq
Forward (+)
51899066 .. 51901156
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1220200.v1.1.491

Sequence Viewer

Length: 549 bp
ATGCCTCCGTCCTCCGACCCCGTCGTCCCCACCGTCACACCAGTCACCATATCCTACTCCGAGCTCCAAGACAGGGACAAAGATTTGTCAGTGAAGATTGAAGAAGGATTTGGACCAAATGGGTTGGGAATCCTATCAATCACTCAAGTTCCGGGATATTCTTCCTTGCGCGGGAATCTTCTAGGCTTGTCACCTAGATTAGCCAATCTGCCCGAAGAGGTGAAGAAGGAACTTGAAGATCCTCATAGTAGGTACAATTTTGGATGGAGTCATGGAAAAGAGAAGCTGGAATCTGGAAAGCCTGATACGTTAAAAGGCTCGTTTTATGCAAATCCAATATTGGATAGCCCTACAACAGATGAATCTCTAATTCAAAGGTATCCATCATACTGTGGTTCAAATATATGGCCCGATAGTGAATTGCCAGAACTAGAAGTCACCTTCAAAGCTCTTGGAAAGGTGTTCCTTGGAGTTGGGCTTATGGTCGCATATCACTGTGACAGATACGGTATGGTCTTTGTTGTTAAGGTTTACACTAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

183

Amino Acids

20.12

Weight (kDa)

5.42

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AccII CGCG 1 cut(s) 171
AciI CCGC 1 cut(s) 171
AclWI GGATC 1 cut(s) 233
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 2 cut(s) 73, 171
AgsI TTSAA 5 cut(s) 101, 236, 374, 399, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
AluBI AGCT 3 cut(s) 64, 286, 449
AluI AGCT 3 cut(s) 64, 286, 449
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 1 cut(s) 233
AoxI GGCC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 113, 408
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 4 cut(s) 37, 183, 232, 430
AvaII GGWCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BccI CCATC 2 cut(s) 258, 391
BciVI GTATCC 1 cut(s) 390
BcnI CCSGG 1 cut(s) 153
BfaI CTAG 3 cut(s) 182, 195, 431
BfuI GTATCC 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 113, 408
BmrFI CCNGG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 466
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 171
Bse1I ACTGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 2 cut(s) 73, 171
BseNI ACTGG 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 11, 89
BslI CCNNNNNNNGG 2 cut(s) 73, 171
BsmFI GGGAC 2 cut(s) 11, 89
BsnI GGCC 1 cut(s) 409
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 1 cut(s) 171
BspANI GGCC 1 cut(s) 409
BspFNI CGCG 1 cut(s) 171
BspPI GGATC 1 cut(s) 233
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 466
Bst4CI ACNGT 4 cut(s) 34, 392, 497, 509
Bst6I CTCTTC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 1 cut(s) 241
BstMBI GATC 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 409
BtsCI GGATG 1 cut(s) 269
BtsIMutI CAGTG 2 cut(s) 96, 493
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 113, 408
Csp6I GTAC 1 cut(s) 253
CviAII CATG 1 cut(s) 272
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 1 cut(s) 537
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 1 cut(s) 275
FaqI GGGAC 2 cut(s) 11, 89
FatI CATG 1 cut(s) 271
FauI CCCGC 1 cut(s) 164
FokI GGATG 1 cut(s) 276
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 3 cut(s) 182, 195, 431
GlaI GCGC 1 cut(s) 170
HaeIII GGCC 1 cut(s) 409
HapII CCGG 1 cut(s) 152
HhaI GCGC 1 cut(s) 171
Hin1II CATG 1 cut(s) 275
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 5 cut(s) 129, 175, 268, 290, 362
HpaII CCGG 1 cut(s) 152
HphI GGTGA 4 cut(s) 37, 183, 232, 430
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 2 cut(s) 16, 61
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 1 cut(s) 532
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 98, 220, 451
HpyCH4III ACNGT 4 cut(s) 34, 392, 497, 509
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 1 cut(s) 329
HpyF3I CTNAG 1 cut(s) 537
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 1 cut(s) 275
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 7 cut(s) 54, 58, 165, 272, 279, 315, 438
MaeI CTAG 3 cut(s) 182, 195, 431
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 5 cut(s) 34, 43, 189, 436, 497
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 7 cut(s) 106, 113, 153, 170, 227, 235, 248
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 3 cut(s) 256, 369, 419
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 4 cut(s) 15, 22, 211, 252
MseI TTAA 2 cut(s) 311, 525
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 171
NciI CCSGG 1 cut(s) 153
NdeII GATC 1 cut(s) 238
NlaIII CATG 1 cut(s) 275
NmuCI GTSAC 5 cut(s) 34, 43, 189, 436, 497
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PfeI GAWTC 4 cut(s) 129, 175, 290, 362
PflFI GACNNNGTC 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 151
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 66
PspPI GGNCC 2 cut(s) 113, 408
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 2 cut(s) 311, 525
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 2 cut(s) 113, 408
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 66
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 3 cut(s) 256, 369, 419
SsiI CCGC 1 cut(s) 171
SspI AATATT 1 cut(s) 339
SspMI CTAG 3 cut(s) 182, 195, 431
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 4 cut(s) 34, 392, 497, 509
TaiI ACGT 1 cut(s) 311
TasI AATT 3 cut(s) 256, 369, 419
TfiI GAWTC 4 cut(s) 129, 175, 290, 362
Tru1I TTAA 2 cut(s) 311, 525
Tru9I TTAA 2 cut(s) 311, 525
TscAI CASTG 2 cut(s) 96, 500
TseFI GTSAC 5 cut(s) 34, 43, 189, 436, 497
Tsp45I GTSAC 5 cut(s) 34, 43, 189, 436, 497
TspDTI ATGAA 1 cut(s) 375
TspRI CASTG 2 cut(s) 96, 500
Tth111I GACNNNGTC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 113
XspI CTAG 3 cut(s) 182, 195, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.