Rh2BG056500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018025
Physical Location & Seq
Forward (+)
4193271 .. 4194920
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG056500.1

Sequence Viewer

Length: 441 bp
ATGCCGCCGACCACCGCCGTCCCCACTCTCACGGTCGTCACCATATCATACACGGAGCTCCAAGACAAGAAGACGGACTTGTCGAGGAAGATTGAAGAAGGATTTGGGCCGAATGGGTTGGGGATTCTATCCGTCACTGATGTTCCGGGATTTTCATCCTTGCGCCAAAATCTTCTACATCTGTCACCTAGGTTGGCCAATCTTCCTGAAGAGGTGAAGAAGGAACTCGAAGATCCTCATAGTAGGTATAATTTTGGATGGAGTCATGGAAAGGAGAAGTTGGAATCTGGAAAGCCAGATATGTTAAAAGGATCTTTCTATGCTAATCCTATATTAGATAGACCAACAACAGATGAGGCTCTAATTCAAAGGTACCCATCGTATTGTGGATCAAATATATGGCCTAAGCGTGAGTTGCCAGAACTTGAAGTGGGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

146

Amino Acids

16.37

Weight (kDa)

5.64

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 20 - 136 2.3e-07 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 79
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 372
AciI CCGC 2 cut(s) 5, 15
AclWI GGATC 3 cut(s) 227, 319, 397
AcoI YGGCCR 1 cut(s) 195
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 1 cut(s) 374
AgsI TTSAA 3 cut(s) 95, 368, 428
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
Alw21I GWGCWC 1 cut(s) 60
AlwI GGATC 3 cut(s) 227, 319, 397
AoxI GGCC 3 cut(s) 107, 195, 401
Asp718I GGTACC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 3 cut(s) 31, 177, 226
AvrII CCTAGG 1 cut(s) 188
BalI TGGCCA 1 cut(s) 197
BanI GGYRCC 1 cut(s) 372
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 60
BccI CCATC 2 cut(s) 252, 385
BceAI ACGGC 1 cut(s) 2
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 1 cut(s) 189
BisI GCNGC 1 cut(s) 5
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 188
Bse8I GATNNNNATC 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 2 cut(s) 155, 263
BseJI GATNNNNATC 1 cut(s) 154
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 3 cut(s) 109, 197, 403
BshNI GGYRCC 1 cut(s) 372
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 5
BsnI GGCC 3 cut(s) 109, 197, 403
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 3 cut(s) 232, 311, 389
BspACI CCGC 2 cut(s) 5, 15
BspANI GGCC 3 cut(s) 109, 197, 403
BspLI GGNNCC 1 cut(s) 374
BspPI GGATC 3 cut(s) 227, 319, 397
BspT107I GGYRCC 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 3 cut(s) 232, 311, 389
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 155, 263
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 3 cut(s) 235, 314, 392
BstMBI GATC 3 cut(s) 232, 311, 389
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 2 cut(s) 232, 311
BstYI RGATCY 2 cut(s) 232, 311
BsuRI GGCC 3 cut(s) 109, 197, 403
BtsCI GGATG 2 cut(s) 155, 263
BtsIMutI CAGTG 1 cut(s) 135
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 373
CviAII CATG 1 cut(s) 266
CviJI RGCY 6 cut(s) 58, 109, 197, 295, 359, 403
CviKI_1 RGCY 6 cut(s) 58, 109, 197, 295, 359, 403
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 1 cut(s) 405
DpnI GATC 3 cut(s) 234, 313, 391
DpnII GATC 3 cut(s) 232, 311, 389
DrdI GACNNNNNNGTC 1 cut(s) 79
DseDI GACNNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 1 cut(s) 269
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 1 cut(s) 5
FatI CATG 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 142, 270
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 3 cut(s) 109, 197, 403
HapII CCGG 1 cut(s) 146
HhaI GCGC 1 cut(s) 165
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 3 cut(s) 124, 262, 284
HpaII CCGG 1 cut(s) 146
HphI GGTGA 3 cut(s) 31, 177, 226
Hpy188III TCNNGA 2 cut(s) 206, 288
HpyAV CCTTC 2 cut(s) 92, 214
HpyCH4III ACNGT 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 1 cut(s) 405
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 376
Kzo9I GATC 3 cut(s) 232, 311, 389
LmnI GCTCC 2 cut(s) 55, 63
LpnPI CCDG 5 cut(s) 159, 219, 273, 309, 432
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 37, 133, 183
MalI GATC 3 cut(s) 234, 313, 391
MboI GATC 3 cut(s) 232, 311, 389
MboII GAAGA 8 cut(s) 82, 100, 107, 164, 194, 221, 229, 242
MflI RGATCY 2 cut(s) 232, 311
MhlI GDGCHC 1 cut(s) 60
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 2 cut(s) 250, 363
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 4 cut(s) 78, 205, 246, 349
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MseI TTAA 1 cut(s) 305
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 415
NciI CCSGG 1 cut(s) 147
NdeII GATC 3 cut(s) 232, 311, 389
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 3 cut(s) 37, 133, 183
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 2 cut(s) 124, 284
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 2 cut(s) 232, 311
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 232, 311, 389
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 60
SetI ASST 6 cut(s) 60, 190, 194, 216, 248, 374
Sse9I AATT 2 cut(s) 250, 363
SsiI CCGC 2 cut(s) 5, 15
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 2 cut(s) 83, 228
TasI AATT 2 cut(s) 250, 363
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 2 cut(s) 124, 284
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 3 cut(s) 37, 133, 183
Tsp45I GTSAC 3 cut(s) 37, 133, 183
TspDTI ATGAA 1 cut(s) 144
TspGWI ACGGA 3 cut(s) 68, 89, 121
TspRI CASTG 1 cut(s) 142
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.