MD02G1050500.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0018025
Physical Location & Seq
Reverse (-)
4178454 .. 4181122
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1050500.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCCTCCGTCCTCCGACCCCGCCGTCCCCACCGTCACACTAGTCACCATATCCTACTCCGAGCTCCAAGACAGGGACAAAGATTTGTCAGTGAAGATTGAAGAAGGATTTGGACCAAATGGGTTGGGAATCCTATCAATCACTCAAGTTCCGGGATATTCTTCCTTGCGCGGGAATCTTCTAGGCTTGTCACCTAGATTAGCCAATCTGCCCGAAGAGGTGAAGAAGGAACTTGAAGATCCTCATAGTAGGTACAATTTTGGATGGAGTCATGGAAAAGAGAAGCTGGAATCTGGAAAGCCTGATACGTTAAAAGGCTCGTTTTATGCAAATCCAATATTGGATAGCCCTACAACAGATAAATCTCTAATTCAAAGGTATCCATCATACTGTGGTTCAAATATATGGCCCAATAGTGAATTGCCAGAACTAGAAGTCACCTTCAAAGCTCTTGGAAAGCTGATCCTTGGAGTTGGGCTTATGGTCGCATATCACTGTGACAGATACGTGTCAAAAGCAATCAAAATGCGCGAGGATGAAGGCCTTGAACAAATACTGTTTCGATCTCGCTGTCATAAAGGGCGTCTACTTTATTATTTTCCAACAAAAGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

206

Amino Acids

22.99

Weight (kDa)

6.91

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 586
AccII CGCG 2 cut(s) 171, 531
AciI CCGC 2 cut(s) 21, 171
AclWI GGATC 2 cut(s) 233, 457
AcyI GRCGYC 1 cut(s) 583
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 2 cut(s) 73, 171
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 6 cut(s) 101, 236, 374, 399, 445, 548
AhlI ACTAGT 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
AluBI AGCT 4 cut(s) 64, 286, 449, 460
AluI AGCT 4 cut(s) 64, 286, 449, 460
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 2 cut(s) 233, 457
AoxI GGCC 2 cut(s) 407, 541
AspLEI GCGC 2 cut(s) 171, 531
AspS9I GGNCC 2 cut(s) 113, 408
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 4 cut(s) 37, 183, 232, 430
AvaII GGWCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BccI CCATC 2 cut(s) 258, 391
BceAI ACGGC 1 cut(s) 8
BciVI GTATCC 1 cut(s) 390
BcnI CCSGG 1 cut(s) 153
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 182, 195, 431
BfuI GTATCC 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 113, 408
BmrFI CCNGG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 466
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 171
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 2 cut(s) 269, 541
BseLI CCNNNNNNNGG 2 cut(s) 73, 171
Bsh1236I CGCG 2 cut(s) 171, 531
BshFI GGCC 2 cut(s) 409, 543
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 11, 89
BslI CCNNNNNNNGG 2 cut(s) 73, 171
BsmFI GGGAC 2 cut(s) 11, 89
BsnI GGCC 2 cut(s) 409, 543
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 3 cut(s) 238, 462, 563
BspACI CCGC 2 cut(s) 21, 171
BspANI GGCC 2 cut(s) 409, 543
BspFNI CGCG 2 cut(s) 171, 531
BspPI GGATC 2 cut(s) 233, 457
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 3 cut(s) 238, 462, 563
BssNI GRCGYC 1 cut(s) 583
BssT1I CCWWGG 1 cut(s) 466
Bst4CI ACNGT 4 cut(s) 34, 392, 497, 558
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 583
BstBAI YACGTR 1 cut(s) 508
BstF5I GGATG 2 cut(s) 269, 541
BstFNI CGCG 2 cut(s) 171, 531
BstHHI GCGC 2 cut(s) 171, 531
BstKTI GATC 3 cut(s) 241, 465, 566
BstMBI GATC 3 cut(s) 238, 462, 563
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 2 cut(s) 171, 531
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 2 cut(s) 409, 543
BtsCI GGATG 2 cut(s) 269, 541
BtsIMutI CAGTG 2 cut(s) 96, 493
CfoI GCGC 2 cut(s) 171, 531
Cfr13I GGNCC 2 cut(s) 113, 408
CseI GACGC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 253
CviAII CATG 1 cut(s) 272
CviQI GTAC 1 cut(s) 253
DpnI GATC 3 cut(s) 240, 464, 565
DpnII GATC 3 cut(s) 238, 462, 563
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 1 cut(s) 275
FaqI GGGAC 2 cut(s) 11, 89
FatI CATG 1 cut(s) 271
FauI CCCGC 2 cut(s) 28, 164
FblI GTMKAC 1 cut(s) 586
FokI GGATG 2 cut(s) 276, 548
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 4 cut(s) 41, 182, 195, 431
GlaI GCGC 2 cut(s) 170, 530
HaeIII GGCC 2 cut(s) 409, 543
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 572
HhaI GCGC 2 cut(s) 171, 531
Hin1I GRCGYC 1 cut(s) 583
Hin1II CATG 1 cut(s) 275
Hin6I GCGC 2 cut(s) 169, 529
HinP1I GCGC 2 cut(s) 169, 529
HinfI GANTC 4 cut(s) 129, 175, 268, 290
HpaII CCGG 1 cut(s) 152
HphI GGTGA 4 cut(s) 37, 183, 232, 430
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 2 cut(s) 16, 61
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 4 cut(s) 98, 220, 451, 533
HpyCH4III ACNGT 4 cut(s) 34, 392, 497, 558
HpyCH4IV ACGT 2 cut(s) 308, 507
HpyCH4V TGCA 1 cut(s) 329
HpySE526I ACGT 2 cut(s) 308, 507
Hsp92I GRCGYC 1 cut(s) 583
Hsp92II CATG 1 cut(s) 275
HspAI GCGC 2 cut(s) 169, 529
Kzo9I GATC 3 cut(s) 238, 462, 563
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 6 cut(s) 58, 165, 272, 279, 315, 438
MaeI CTAG 4 cut(s) 41, 182, 195, 431
MaeII ACGT 2 cut(s) 308, 507
MaeIII GTNAC 5 cut(s) 34, 43, 189, 436, 497
MalI GATC 3 cut(s) 240, 464, 565
MboI GATC 3 cut(s) 238, 462, 563
MboII GAAGA 7 cut(s) 106, 113, 153, 170, 227, 235, 248
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 3 cut(s) 256, 369, 419
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 5 cut(s) 15, 22, 211, 252, 526
MseI TTAA 1 cut(s) 311
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 153
MvnI CGCG 2 cut(s) 171, 531
NciI CCSGG 1 cut(s) 153
NdeII GATC 3 cut(s) 238, 462, 563
NlaIII CATG 1 cut(s) 275
NmuCI GTSAC 5 cut(s) 34, 43, 189, 436, 497
PceI AGGCCT 1 cut(s) 543
PfeI GAWTC 3 cut(s) 129, 175, 290
PfoI TCCNGGA 1 cut(s) 151
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Ppu21I YACGTR 1 cut(s) 508
Psp124BI GAGCTC 1 cut(s) 66
PspPI GGNCC 2 cut(s) 113, 408
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 1 cut(s) 311
Sau3AI GATC 3 cut(s) 238, 462, 563
Sau96I GGNCC 2 cut(s) 113, 408
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 66
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 3 cut(s) 256, 369, 419
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 2 cut(s) 21, 171
SspI AATATT 1 cut(s) 339
SspMI CTAG 4 cut(s) 41, 182, 195, 431
SstI GAGCTC 1 cut(s) 66
StuI AGGCCT 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 4 cut(s) 34, 392, 497, 558
TaiI ACGT 2 cut(s) 311, 510
TaqI TCGA 1 cut(s) 562
TasI AATT 3 cut(s) 256, 369, 419
TfiI GAWTC 3 cut(s) 129, 175, 290
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 2 cut(s) 96, 500
TseFI GTSAC 5 cut(s) 34, 43, 189, 436, 497
Tsp45I GTSAC 5 cut(s) 34, 43, 189, 436, 497
TspDTI ATGAA 1 cut(s) 552
TspRI CASTG 2 cut(s) 96, 500
VpaK11BI GGWCC 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 586
XspI CTAG 4 cut(s) 41, 182, 195, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.