MD01G1003600.v1.1

Cyanobacterial and plant NDH-1 subunit O

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012164
Physical Location & Seq
Forward (+)
1204432 .. 1206849
2418 bp
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UTR
Exon/CDS
Intron
MD01G1003600.v1.1.491

Sequence Viewer

Length: 591 bp
ATGTACCACAGAAGGACTCCTTATCGTCTTGTTTTCTCTGTTGAGGATTTAATTATCTGTGTGTTTCTCCATCAAGATTCAATTGTCATGGCTTTCTCTGCGAGTAGTTGTACTTGTACCCTCTCTAAGAACCCTTTCTCATGCTTGTCTCCTTTCCCACGAACCCTGGGAAGAAACCCTACTCGTCTTTTGTCCATTAAAGCTGTAAAAGCTACAGAAACAGAAGCCAATAATAATAATACCGAGAAGCCGTCTTCGTCTTCCAATGCTCAACCTTCAACCGCCAAGCCGCCTCCCAAGAAGAAGCCTGTCTACTCCATGAAGAAGGGTCAGATTGTGAGGGTGGAGAAAGACAAATATCTTAATAGTGTTAATTATCTTTCGGTTGGACATCCACCGTATTACAAAGGATTGGACTATATTTATGAAGACCGCGGTGAGATATTGGATTTGCGTATTTTTGAGACAGGAGAGTATGCACTTGTAGCATGGGTTGGGGTCCCAACTGCGCCAGCATGGCTTCCAACAGACATGCTTATCAGGGTACCTGCGTGCTTCTTTCTTGCTAAGTTGGAAATTAGACTAGTTTAA

Protein Analysis

197

Amino Acids

22.11

Weight (kDa)

9.27

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhO PF11910 107 - 180 1.7e-21 Cyanobacterial and plant NDH-1 subunit O
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74880
fragaria_vesca FvH4_1g24330 FvH4_1g24330 FvH4_1g24330
malus_domestica MD01G1003600.v1.1
prunus_persica Prupe.6G157800_v2.0.a1
pyrus_communis pycom01g02350
rosa_chinensis RchiOBHm_Chr2g0120841
rosa_laevigata RLG00000018594
rosa_multiflora Rmu_sc0000621.1_g000009
rosa_roxburghii Rroxscaffold_2G00122790
rosa_rugosa Rorug02G0232000 Rorug02G0232000 Rorug02G0232100
rosa_samantha Rh2AG288600 Rh2BG299600 Rh2CG277600 Rh2DG313700
rosa_wichuraiana Rw2G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 556
Acc65I GGTACC 1 cut(s) 544
AccB1I GGYRCC 1 cut(s) 544
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 435
AciI CCGC 4 cut(s) 282, 290, 433, 435
AfaI GTAC 4 cut(s) 5, 112, 118, 546
AgsI TTSAA 2 cut(s) 81, 279
AhlI ACTAGT 1 cut(s) 583
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 2 cut(s) 203, 212
AluI AGCT 2 cut(s) 203, 212
Alw26I GTCTC 2 cut(s) 153, 458
Asp700I GAANNNNTTC 1 cut(s) 134
Asp718I GGTACC 1 cut(s) 544
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 1 cut(s) 499
AsuHPI GGTGA 1 cut(s) 449
AvaII GGWCC 1 cut(s) 499
BaeI ACNNNNGTAYC 2 cut(s) 536, 569
BanI GGYRCC 1 cut(s) 544
BarI GAAGNNNNNNTAC 4 cut(s) 163, 195, 296, 328
BbsI GAAGAC 3 cut(s) 246, 252, 435
BccI CCATC 1 cut(s) 78
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 167
BcoDI GTCTC 2 cut(s) 153, 458
BcuI ACTAGT 1 cut(s) 583
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 556
BglI GCCNNNNNGGC 1 cut(s) 517
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 3 cut(s) 500, 501, 546
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 3 cut(s) 246, 252, 435
BsaJI CCNNGG 3 cut(s) 165, 166, 433
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 3 cut(s) 165, 166, 433
BseGI GGATG 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 435
BshNI GGYRCC 1 cut(s) 544
BslFI GGGAC 1 cut(s) 485
BsmAI GTCTC 2 cut(s) 153, 458
BsmFI GGGAC 1 cut(s) 485
BspACI CCGC 4 cut(s) 282, 290, 433, 435
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 500, 501, 546
BspMI ACCTGC 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 544
BssECI CCNNGG 3 cut(s) 165, 166, 433
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 399
BstC8I GCNNGC 2 cut(s) 513, 553
BstDEI CTNAG 2 cut(s) 126, 567
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 511
BstMAI GTCTC 2 cut(s) 153, 458
BstMWI GCNNNNNNNGC 4 cut(s) 98, 209, 485, 517
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 1 cut(s) 435
BstV2I GAAGAC 3 cut(s) 246, 252, 435
BtgI CCRYGG 1 cut(s) 433
BtsCI GGATG 1 cut(s) 391
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 513, 553
CfoI GCGC 1 cut(s) 511
Cfr13I GGNCC 1 cut(s) 499
Cfr42I CCGCGG 1 cut(s) 436
Csp6I GTAC 4 cut(s) 4, 111, 117, 545
CviAII CATG 6 cut(s) 88, 141, 319, 489, 516, 532
CviJI RGCY 8 cut(s) 92, 203, 212, 227, 250, 289, 307, 520
CviKI_1 RGCY 8 cut(s) 92, 203, 212, 227, 250, 289, 307, 520
CviQI GTAC 4 cut(s) 4, 111, 117, 545
DdeI CTNAG 2 cut(s) 126, 567
Eco47I GGWCC 1 cut(s) 499
EcoO109I RGGNCCY 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 165
FaeI CATG 6 cut(s) 91, 144, 322, 492, 519, 535
FaiI YATR 9 cut(s) 89, 142, 320, 420, 426, 477, 490, 517, 533
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 1 cut(s) 485
FatI CATG 6 cut(s) 87, 140, 318, 488, 515, 531
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 1 cut(s) 378
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 510
GluI GCNGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 511
Hin1II CATG 6 cut(s) 91, 144, 322, 492, 519, 535
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HinfI GANTC 2 cut(s) 16, 77
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 1 cut(s) 333
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 1 cut(s) 313
HpyAV CCTTC 3 cut(s) 6, 285, 319
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4V TGCA 1 cut(s) 479
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 209, 485, 517
HpyF3I CTNAG 2 cut(s) 126, 567
Hsp92II CATG 6 cut(s) 91, 144, 322, 492, 519, 535
HspAI GCGC 1 cut(s) 509
KflI GGGWCCC 1 cut(s) 499
KpnI GGTACC 1 cut(s) 548
KspI CCGCGG 1 cut(s) 436
LpnPI CCDG 7 cut(s) 152, 179, 321, 453, 525, 526, 561
MaeI CTAG 1 cut(s) 584
MboII GAAGA 6 cut(s) 183, 246, 252, 313, 334, 440
MfeI CAATTG 1 cut(s) 81
MluCI AATT 4 cut(s) 51, 81, 373, 576
MlyI GAGTC 1 cut(s) 10
MmeI TCCRAC 3 cut(s) 367, 548, 552
MnlI CCTC 4 cut(s) 37, 131, 303, 333
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 5 cut(s) 50, 198, 363, 372, 589
MspA1I CMGCKG 1 cut(s) 435
MspR9I CCNGG 1 cut(s) 167
MunI CAATTG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 98, 209, 485, 517
NlaIII CATG 6 cut(s) 91, 144, 322, 492, 519, 535
NlaIV GGNNCC 3 cut(s) 500, 501, 546
NspI RCATGY 1 cut(s) 535
PasI CCCWGGG 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 499
Psp5II RGGWCCY 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 500, 501, 546
PspPI GGNCC 1 cut(s) 499
PspPPI RGGWCCY 1 cut(s) 499
RsaI GTAC 4 cut(s) 5, 112, 118, 546
RsaNI GTAC 4 cut(s) 4, 111, 117, 545
SacII CCGCGG 1 cut(s) 436
SaqAI TTAA 5 cut(s) 50, 198, 363, 372, 589
SatI GCNGC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 499
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 167
SetI ASST 4 cut(s) 205, 214, 277, 550
SfcI CTRYAG 1 cut(s) 213
Sfr303I CCGCGG 1 cut(s) 436
SgrBI CCGCGG 1 cut(s) 436
SinI GGWCC 1 cut(s) 499
SpeI ACTAGT 1 cut(s) 583
Sse9I AATT 4 cut(s) 51, 81, 373, 576
SsiI CCGC 4 cut(s) 282, 290, 433, 435
SspMI CTAG 1 cut(s) 584
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 399
TasI AATT 4 cut(s) 51, 81, 373, 576
TatI WGTACW 1 cut(s) 110
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 5 cut(s) 50, 198, 363, 372, 589
Tru9I TTAA 5 cut(s) 50, 198, 363, 372, 589
TspDTI ATGAA 2 cut(s) 335, 441
VpaK11BI GGWCC 1 cut(s) 499
XceI RCATGY 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.