MD01G1005700.v1.1

histone deacetylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006797
Physical Location & Seq
Reverse (-)
2116071 .. 2126575
10505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1005700.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGGAACTCCTTTCATTCCCAATTTCGTCATCACCCACTGGTGGGAATGCGTTGTTTTTGGTACGAAAGCATTTGTGCAAGTGGAAGAGCCATAGTAGATTTGACTTGGATGCAAAATTTGTGAGAGACAGAATTTTGAATAAGAGTTGGCGACGAAGGAGATGTTTCTCTGAAAAAGCAGAAACTCCTATTTCTTGTTCGAATGGTACAGGGGAGGATCCCTTTATACCTGTTAATAAGAAATTAAGTGATGCACGTGTGATTTATGCTGTAGCTCCTGCCATGGGCCATAATAAGGAGGCTCATCCAGAATCTCATTTTAGAGTTCCTGCAATAGTGAATGCTCTTGAAAAGTTGGAGCTCTCGCCTAAGTTTCGTGGCTCAAATGTTATTGAACTTGAAACTTTCAAGTCAGCATCATTGGATGACATAGCAAGTGTTCATACAAGAGCTTATGTATCAGGCCTGGAGAAGGCTATGGATGAAGCCTCACAACAGGGAATTATTTTTATTGAAGGTTCTGGACCAACATATGCAACTGTTGATACGTTCCAGGAGTCCCTTGTTGCAGCTGGAGCAGGAATTTCCTTAATCGATTCAGTGGTCGCACAATCAAAGATCAGCAAGAGTCCACCTATAGGTTTTGCTTTGATAAGACCTCCCGGACACCATGCTATTCCAAAGGGTCCAATGGGGTTTTGTGTTTTTGGAAATATTGCAATTGCAGCTCGTTATGCTCAGCGCGTGCATGGCTTAAAGCGTGTCTTTATCATCGATTTTGATGTCCATCATGGGAATGGGACAAACGATGCTTTCTATGAGGATCCAGATATTTTTTTCCTGTCAACTCACCAAGATGGAAGCTACCCTGGAACTGGTAAGTTTGACGAGGTAGGTCATGGCGATGGGGAAGGAACAACACTAAATCTTCCTCTACCTGGAGGCTCAGGTGATGCTGCCATGAGAGCTATCTTTGATGAAATAATTGTACCATGTGCTCAGAGGTTTAAGCCAGATATCATTCTTGTCTCTGCCGGGTACGATGCTCATGTATTGGATCCACTAGCTAGTCTGCAGTTCACGACGGGAACATACTACATGCTGGCATCCAATATTCAACAACTTGCAAAAGATGTATGTGGGGGTCGATGCGTGTTTTTCTTGGAAGGAGGGTACAACCTCGAGTCGCTTTCATATTCAGTGGCTGATTCATTCCGAGCCTTTCTTGGAGAACCGAGTTTGGCGTCCGAGTTTGACAACCCCGCCTTCTTGCATGAAGAGCCATCAACCAGGGTTAGGCAGGCAATTCAGAGGGTGAAACAAATACATTCCTTGTGA

Protein Analysis

446

Amino Acids

48.58

Weight (kDa)

6.25

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hist_deacetyl PF00850 102 - 406 9.1e-90 Histone deacetylase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 744
AciI CCGC 1 cut(s) 1263
AclWI GGATC 6 cut(s) 212, 225, 818, 831, 1052, 1065
AcsI RAATTY 3 cut(s) 116, 132, 582
AcvI CACGTG 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 1244
AfaI GTAC 5 cut(s) 63, 208, 990, 1040, 1175
AfiI CCNNNNNNNGG 9 cut(s) 41, 42, 284, 295, 472, 638, 875, 938, 1296
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 7 cut(s) 139, 350, 395, 401, 409, 515, 1118
AjnI CCWGG 5 cut(s) 465, 552, 868, 937, 1289
AluBI AGCT 8 cut(s) 275, 361, 452, 572, 728, 864, 968, 1067
AluI AGCT 8 cut(s) 275, 361, 452, 572, 728, 864, 968, 1067
Alw21I GWGCWC 2 cut(s) 363, 1000
Alw26I GTCTC 2 cut(s) 120, 1033
AlwI GGATC 6 cut(s) 212, 225, 818, 831, 1052, 1065
AlwNI CAGNNNCTG 1 cut(s) 1205
Ama87I CYCGRG 1 cut(s) 1181
AoxI GGCC 2 cut(s) 286, 463
ApeKI GCWGC 3 cut(s) 569, 725, 956
ApoI RAATTY 3 cut(s) 116, 132, 582
AspLEI GCGC 1 cut(s) 744
AspS9I GGNCC 3 cut(s) 286, 524, 686
AsuC2I CCSGG 2 cut(s) 663, 1036
AsuHPI GGTGA 4 cut(s) 24, 842, 962, 1327
AsuII TTCGAA 1 cut(s) 200
AvaI CYCGRG 1 cut(s) 1181
AvaII GGWCC 2 cut(s) 524, 686
BamHI GGATCC 3 cut(s) 217, 823, 1057
BanII GRGCYC 1 cut(s) 363
BbrPI CACGTG 1 cut(s) 257
Bbv12I GWGCWC 2 cut(s) 363, 1000
BbvI GCAGC 3 cut(s) 581, 737, 943
BccI CCATC 4 cut(s) 795, 851, 899, 1291
BciT130I CCWGG 5 cut(s) 467, 554, 870, 939, 1291
BcnI CCSGG 2 cut(s) 663, 1036
BcoDI GTCTC 2 cut(s) 120, 1033
BfaI CTAG 2 cut(s) 1064, 1068
BfmI CTRYAG 3 cut(s) 270, 636, 1073
BisI GCNGC 3 cut(s) 570, 726, 957
BlpI GCTNAGC 1 cut(s) 738
BlsI GCNGC 3 cut(s) 571, 727, 958
Bme1390I CCNGG 7 cut(s) 467, 554, 663, 870, 939, 1036, 1291
Bme18I GGWCC 2 cut(s) 524, 686
BmeT110I CYCGRG 1 cut(s) 1181
BmgT120I GGNCC 3 cut(s) 286, 524, 686
BmiI GGNNCC 4 cut(s) 219, 687, 825, 1059
BmrFI CCNGG 7 cut(s) 467, 554, 663, 870, 939, 1036, 1291
BmsI GCATC 8 cut(s) 100, 241, 425, 799, 943, 1033, 1115, 1139
BpmI CTGGAG 3 cut(s) 488, 594, 960
Bpu10I CCTNAGC 1 cut(s) 946
Bpu1102I GCTNAGC 1 cut(s) 738
Bpu14I TTCGAA 1 cut(s) 200
BpuMI CCSGG 2 cut(s) 663, 1036
Bsa29I ATCGAT 2 cut(s) 594, 774
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 1244
BsaJI CCNNGG 3 cut(s) 282, 868, 1290
Bsc4I CCNNNNNNNGG 9 cut(s) 41, 42, 284, 295, 472, 638, 875, 938, 1296
Bse1I ACTGG 2 cut(s) 43, 880
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 5 cut(s) 467, 554, 870, 939, 1291
BseCI ATCGAT 2 cut(s) 594, 774
BseDI CCNNGG 3 cut(s) 282, 868, 1290
BseGI GGATG 5 cut(s) 115, 304, 430, 487, 1106
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 9 cut(s) 41, 42, 284, 295, 472, 638, 875, 938, 1296
BseMII CTCAG 3 cut(s) 752, 960, 1013
BseNI ACTGG 2 cut(s) 43, 880
BseXI GCAGC 3 cut(s) 581, 737, 943
Bsh1236I CGCG 1 cut(s) 744
BshFI GGCC 2 cut(s) 288, 465
BshVI ATCGAT 2 cut(s) 594, 774
BsiHKAI GWGCWC 2 cut(s) 363, 1000
BsiHKCI CYCGRG 1 cut(s) 1181
BsiSI CCGG 2 cut(s) 663, 1035
BslFI GGGAC 2 cut(s) 544, 814
BslI CCNNNNNNNGG 9 cut(s) 41, 42, 284, 295, 472, 638, 875, 938, 1296
BsmAI GTCTC 2 cut(s) 120, 1033
BsmFI GGGAC 2 cut(s) 544, 814
BsmI GAATGC 2 cut(s) 52, 346
BsnI GGCC 2 cut(s) 288, 465
BsoBI CYCGRG 1 cut(s) 1181
Bsp119I TTCGAA 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 363, 1000
Bsp143I GATC 4 cut(s) 217, 618, 823, 1057
Bsp1720I GCTNAGC 1 cut(s) 738
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 1 cut(s) 1263
BspANI GGCC 2 cut(s) 288, 465
BspCNI CTCAG 3 cut(s) 751, 959, 1012
BspDI ATCGAT 2 cut(s) 594, 774
BspFNI CGCG 1 cut(s) 744
BspLI GGNNCC 4 cut(s) 219, 687, 825, 1059
BspMAI CTGCAG 1 cut(s) 1077
BspPI GGATC 6 cut(s) 212, 225, 818, 831, 1052, 1065
BspQI GCTCTTC 2 cut(s) 80, 1272
BspT104I TTCGAA 1 cut(s) 200
BsrI ACTGG 2 cut(s) 43, 880
BssECI CCNNGG 3 cut(s) 282, 868, 1290
BssMI GATC 4 cut(s) 217, 618, 823, 1057
BssNI GRCGYC 1 cut(s) 1244
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 5 cut(s) 467, 554, 870, 939, 1291
Bst4CI ACNGT 1 cut(s) 541
Bst6I CTCTTC 2 cut(s) 80, 1272
BstACI GRCGYC 1 cut(s) 1244
BstBAI YACGTR 1 cut(s) 257
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 3 cut(s) 746, 1104, 1302
BstDEI CTNAG 4 cut(s) 369, 738, 946, 999
BstDSI CCRYGG 1 cut(s) 282
BstENI CCTNNNNNAGG 1 cut(s) 470
BstF5I GGATG 5 cut(s) 115, 304, 430, 487, 1106
BstFNI CGCG 1 cut(s) 744
BstHHI GCGC 1 cut(s) 744
BstKTI GATC 4 cut(s) 220, 621, 826, 1060
BstMAI GTCTC 2 cut(s) 120, 1033
BstMBI GATC 4 cut(s) 217, 618, 823, 1057
BstMWI GCNNNNNNNGC 6 cut(s) 575, 725, 734, 750, 965, 1279
BstNI CCWGG 5 cut(s) 467, 554, 870, 939, 1291
BstNSI RCATGY 1 cut(s) 1102
BstSCI CCNGG 7 cut(s) 465, 552, 661, 868, 937, 1034, 1289
BstSFI CTRYAG 3 cut(s) 270, 636, 1073
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 581, 737, 943
BstX2I RGATCY 3 cut(s) 217, 823, 1057
BstYI RGATCY 3 cut(s) 217, 823, 1057
Bsu15I ATCGAT 2 cut(s) 594, 774
BsuRI GGCC 2 cut(s) 288, 465
BsuTUI ATCGAT 2 cut(s) 594, 774
BtgI CCRYGG 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 5 cut(s) 115, 304, 430, 487, 1106
BtsIMutI CAGTG 3 cut(s) 36, 606, 1206
Cac8I GCNNGC 3 cut(s) 746, 1104, 1302
CaiI CAGNNNCTG 1 cut(s) 1205
CfoI GCGC 1 cut(s) 744
Cfr13I GGNCC 3 cut(s) 286, 524, 686
ClaI ATCGAT 2 cut(s) 594, 774
CseI GACGC 1 cut(s) 1233
Csp6I GTAC 5 cut(s) 62, 207, 989, 1039, 1174
CviQI GTAC 5 cut(s) 62, 207, 989, 1039, 1174
DdeI CTNAG 4 cut(s) 369, 738, 946, 999
DpnI GATC 4 cut(s) 219, 620, 825, 1059
DpnII GATC 4 cut(s) 217, 618, 823, 1057
Eam1104I CTCTTC 2 cut(s) 80, 1272
EarI CTCTTC 2 cut(s) 80, 1272
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 363
Eco32I GATATC 1 cut(s) 1018
Eco47I GGWCC 2 cut(s) 524, 686
Eco53kI GAGCTC 1 cut(s) 361
Eco72I CACGTG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 1181
EcoICRI GAGCTC 1 cut(s) 361
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoRII CCWGG 5 cut(s) 465, 552, 868, 937, 1289
EcoRV GATATC 1 cut(s) 1018
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 282
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 2 cut(s) 544, 814
FauI CCCGC 1 cut(s) 1270
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 570, 726, 957
FokI GGATG 5 cut(s) 122, 291, 437, 494, 1093
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 3 cut(s) 570, 726, 957
FspBI CTAG 2 cut(s) 1064, 1068
GlaI GCGC 1 cut(s) 743
GluI GCNGC 3 cut(s) 570, 726, 957
GsuI CTGGAG 3 cut(s) 488, 594, 960
HaeIII GGCC 2 cut(s) 288, 465
HapII CCGG 2 cut(s) 663, 1035
HgaI GACGC 1 cut(s) 1233
HhaI GCGC 1 cut(s) 744
Hin1I GRCGYC 1 cut(s) 1244
Hin6I GCGC 1 cut(s) 742
HinP1I GCGC 1 cut(s) 742
HincII GTYRAC 1 cut(s) 846
HindII GTYRAC 1 cut(s) 846
HinfI GANTC 6 cut(s) 311, 557, 596, 628, 1184, 1208
HpaII CCGG 2 cut(s) 663, 1035
HphI GGTGA 4 cut(s) 24, 842, 962, 1327
Hpy166II GTNNAC 3 cut(s) 632, 846, 1080
Hpy188I TCNGA 5 cut(s) 172, 1002, 1217, 1249, 1311
Hpy188III TCNNGA 5 cut(s) 308, 347, 522, 827, 1081
Hpy8I GTNNAC 3 cut(s) 632, 846, 1080
Hpy99I CGWCG 2 cut(s) 156, 1087
HpyAV CCTTC 6 cut(s) 150, 466, 509, 905, 1160, 1276
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 2 cut(s) 256, 548
HpyF10VI GCNNNNNNNGC 6 cut(s) 575, 725, 734, 750, 965, 1279
HpyF3I CTNAG 4 cut(s) 369, 738, 946, 999
HpySE526I ACGT 2 cut(s) 256, 548
Hsp92I GRCGYC 1 cut(s) 1244
HspAI GCGC 1 cut(s) 742
Kzo9I GATC 4 cut(s) 217, 618, 823, 1057
LguI GCTCTTC 2 cut(s) 80, 1272
LmnI GCTCC 3 cut(s) 280, 358, 575
Lsp1109I GCAGC 3 cut(s) 581, 737, 943
LweI GCATC 8 cut(s) 100, 241, 425, 799, 943, 1033, 1115, 1139
MaeI CTAG 2 cut(s) 1064, 1068
MaeII ACGT 2 cut(s) 256, 548
MalI GATC 4 cut(s) 219, 620, 825, 1059
MboI GATC 4 cut(s) 217, 618, 823, 1057
MboII GAAGA 3 cut(s) 97, 920, 1289
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 3 cut(s) 217, 823, 1057
MhlI GDGCHC 2 cut(s) 363, 1000
MluCI AATT 9 cut(s) 21, 116, 132, 242, 501, 582, 720, 984, 1305
MlyI GAGTC 3 cut(s) 566, 637, 1193
MmeI TCCRAC 1 cut(s) 336
MseI TTAA 5 cut(s) 234, 245, 590, 755, 1008
MslI CAYNNNNRTG 3 cut(s) 795, 855, 903
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 2 cut(s) 663, 1035
MspR9I CCNGG 7 cut(s) 467, 554, 663, 870, 939, 1036, 1291
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 2 cut(s) 52, 346
MvaI CCWGG 5 cut(s) 467, 554, 870, 939, 1291
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 6 cut(s) 575, 725, 734, 750, 965, 1279
NciI CCSGG 2 cut(s) 663, 1036
NcoI CCATGG 1 cut(s) 282
NdeI CATATG 1 cut(s) 532
NdeII GATC 4 cut(s) 217, 618, 823, 1057
NlaIV GGNNCC 4 cut(s) 219, 687, 825, 1059
NspI RCATGY 1 cut(s) 1102
NspV TTCGAA 1 cut(s) 200
PaeR7I CTCGAG 1 cut(s) 1181
PceI AGGCCT 1 cut(s) 465
PciSI GCTCTTC 2 cut(s) 80, 1272
PctI GAATGC 2 cut(s) 52, 346
PfeI GAWTC 3 cut(s) 311, 596, 1208
PfoI TCCNGGA 2 cut(s) 552, 661
PkrI GCNGC 3 cut(s) 571, 727, 958
PleI GAGTC 3 cut(s) 565, 636, 1192
PmaCI CACGTG 1 cut(s) 257
PmlI CACGTG 1 cut(s) 257
PpsI GAGTC 3 cut(s) 565, 636, 1192
Ppu21I YACGTR 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 5 cut(s) 465, 552, 868, 937, 1289
PspCI CACGTG 1 cut(s) 257
PspGI CCWGG 5 cut(s) 465, 552, 868, 937, 1289
PspN4I GGNNCC 4 cut(s) 219, 687, 825, 1059
PspPI GGNCC 3 cut(s) 286, 524, 686
PspXI VCTCGAGB 1 cut(s) 1181
PstI CTGCAG 1 cut(s) 1077
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 3 cut(s) 217, 823, 1057
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 5 cut(s) 63, 208, 990, 1040, 1175
RsaNI GTAC 5 cut(s) 62, 207, 989, 1039, 1174
RseI CAYNNNNRTG 3 cut(s) 795, 855, 903
SacI GAGCTC 1 cut(s) 363
SapI GCTCTTC 2 cut(s) 80, 1272
SaqAI TTAA 5 cut(s) 234, 245, 590, 755, 1008
SatI GCNGC 3 cut(s) 570, 726, 957
Sau3AI GATC 4 cut(s) 217, 618, 823, 1057
Sau96I GGNCC 3 cut(s) 286, 524, 686
SchI GAGTC 3 cut(s) 566, 637, 1193
ScrFI CCNGG 7 cut(s) 467, 554, 663, 870, 939, 1036, 1291
SduI GDGCHC 2 cut(s) 363, 1000
SfaNI GCATC 8 cut(s) 100, 241, 425, 799, 943, 1033, 1115, 1139
SfcI CTRYAG 3 cut(s) 270, 636, 1073
Sfr274I CTCGAG 1 cut(s) 1181
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 2 cut(s) 524, 686
SlaI CTCGAG 1 cut(s) 1181
SmiMI CAYNNNNRTG 3 cut(s) 795, 855, 903
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
Sse9I AATT 9 cut(s) 21, 116, 132, 242, 501, 582, 720, 984, 1305
SseBI AGGCCT 1 cut(s) 465
SsiI CCGC 1 cut(s) 1263
SspI AATATT 2 cut(s) 715, 1114
SspMI CTAG 2 cut(s) 1064, 1068
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 465
StyD4I CCNGG 7 cut(s) 465, 552, 661, 868, 937, 1034, 1289
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 2 cut(s) 259, 551
TaqI TCGA 5 cut(s) 200, 594, 774, 1147, 1182
TasI AATT 9 cut(s) 21, 116, 132, 242, 501, 582, 720, 984, 1305
TfiI GAWTC 3 cut(s) 311, 596, 1208
Tru1I TTAA 5 cut(s) 234, 245, 590, 755, 1008
Tru9I TTAA 5 cut(s) 234, 245, 590, 755, 1008
TscAI CASTG 3 cut(s) 43, 606, 1206
TseI GCWGC 3 cut(s) 569, 725, 956
TspDTI ATGAA 6 cut(s) 431, 498, 993, 1182, 1200, 1290
TspRI CASTG 3 cut(s) 43, 606, 1206
VpaK11BI GGWCC 2 cut(s) 524, 686
XagI CCTNNNNNAGG 1 cut(s) 470
XapI RAATTY 3 cut(s) 116, 132, 582
XceI RCATGY 1 cut(s) 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XhoI CTCGAG 1 cut(s) 1181
XspI CTAG 2 cut(s) 1064, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.