MD01G1007300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012333
Physical Location & Seq
Forward (+)
3700477 .. 3701127
651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1007300.v1.1.491

Sequence Viewer

Length: 651 bp
ATGCTGTCGCCACCCTCCTCCCCTACCAACTCCTCCGTCTCCTCCTCCGATCTCGACACCGAGTCTACAGGCTCCTTCTTTCACGATCGGAGCACGACGTTAGGAACACTAATGGGAGTGAGCTTCCCCGCCATCACCTTCAGAGTTCCGAGCCAGCATCGCCACGACCCCCAAATCGAAAACATCAACAACATCGGTAGTACTAGCGGAAGCAGCTCCGGGAAGACGAAGAAGCCGAAAAAGAAGAGCACCGCCGCTTCGTTGGCGGTGGGTGTGCGGGCGGGGCGACGGAAGAGGTGGTGGCGCCTCTGCCAAGATGACGGCACCAAGCCGGCTTCGCTTGGGGAGTTTCTTGAGGTGGAGAGGAGGTTCGGATATGCTGCATTTTACTATACGGTCGCGGAACTGGAAGGCGTCATGGTGGGCCAGCCTAGAAATGGACGGCTGTTGTTCGTCAACGGGAGGGTTCTTCTGCCATCGAATGTTGATGATGGGATTAGGACTTCATCGACGGCTGGGGTTTTCTCTAGATTGCTAGTTTCACTCACTGCCATCTGTGGTGGCGGTGTAGCATTGATGAAAAGAGGAAGTGATCAGCAACAAGCAGGTGCTACTGAAAGACTATATGACCGGCTTCCCCAAAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

23.36

Weight (kDa)

10.06

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17950 AT3G17950 AT3G17950
fragaria_vesca FvH4_5g17460
malus_domestica MD01G1007300.v1.1 MD06G1228000.v1.1 MD14G1237200.v1.1
prunus_persica Prupe.5G234500_v2.0.a1
pyrus_communis pycom06g20440 pycom14g19820
rosa_chinensis RchiOBHm_Chr7g0178231
rosa_multiflora Rmu_sc0007855.1_g000003
rosa_roxburghii Rroxscaffold_3G00275060
rosa_rugosa Rorug06G0415200
rosa_samantha Rh7AG014300 Rh7BG013800 Rh7CG013700 Rh7DG014000
rosa_wichuraiana Rw7G001120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccB1I GGYRCC 2 cut(s) 303, 323
AccI GTMKAC 1 cut(s) 65
AccII CGCG 1 cut(s) 401
AciI CCGC 9 cut(s) 129, 207, 252, 255, 266, 277, 281, 401, 564
AcuI CTGAAG 1 cut(s) 124
AcyI GRCGYC 2 cut(s) 304, 414
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 1 cut(s) 437
AhdI GACNNNNNGTC 1 cut(s) 61
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 2 cut(s) 123, 216
AluI AGCT 2 cut(s) 123, 216
Alw21I GWGCWC 2 cut(s) 95, 251
Alw26I GTCTC 1 cut(s) 43
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 213, 380
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 127
BanI GGYRCC 2 cut(s) 303, 323
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 95, 251
BbvI GCAGC 2 cut(s) 225, 367
BccI CCATC 4 cut(s) 140, 484, 485, 560
BceAI ACGGC 3 cut(s) 337, 458, 528
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 4 cut(s) 204, 432, 528, 536
BfmI CTRYAG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 307
BfuAI ACCTGC 1 cut(s) 596
BisI GCNGC 3 cut(s) 214, 255, 381
BlsI GCNGC 3 cut(s) 215, 256, 382
BmcAI AGTACT 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 220
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 3 cut(s) 73, 305, 325
BmrFI CCNGG 1 cut(s) 220
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 220
BsaHI GRCGYC 2 cut(s) 304, 414
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse118I RCCGGY 2 cut(s) 331, 630
Bse1I ACTGG 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 5 cut(s) 7, 22, 31, 34, 379
BseXI GCAGC 2 cut(s) 225, 367
BseYI CCCAGC 1 cut(s) 515
Bsh1236I CGCG 1 cut(s) 401
Bsh1285I CGRYCG 2 cut(s) 88, 399
BshFI GGCC 1 cut(s) 426
BshNI GGYRCC 2 cut(s) 303, 323
BsiEI CGRYCG 2 cut(s) 88, 399
BsiHKAI GWGCWC 2 cut(s) 95, 251
BsiSI CCGG 3 cut(s) 219, 332, 631
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 43
BsmBI CGTCTC 1 cut(s) 43
BsnI GGCC 1 cut(s) 426
Bsp1286I GDGCHC 2 cut(s) 95, 251
Bsp143I GATC 3 cut(s) 49, 85, 592
BspACI CCGC 9 cut(s) 129, 207, 252, 255, 266, 277, 281, 401, 564
BspANI GGCC 1 cut(s) 426
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 73, 305, 325
BspMI ACCTGC 1 cut(s) 596
BspQI GCTCTTC 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 303, 323
BsrFI RCCGGY 2 cut(s) 331, 630
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 2 cut(s) 331, 630
BssMI GATC 3 cut(s) 49, 85, 592
BssNI GRCGYC 2 cut(s) 304, 414
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 2 cut(s) 239, 287
BstACI GRCGYC 2 cut(s) 304, 414
BstC8I GCNNGC 4 cut(s) 155, 279, 333, 428
BstFNI CGCG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 3 cut(s) 52, 88, 595
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 3 cut(s) 49, 85, 592
BstMCI CGRYCG 2 cut(s) 88, 399
BstMWI GCNNNNNNNGC 5 cut(s) 159, 213, 263, 283, 337
BstSCI CCNGG 1 cut(s) 218
BstSFI CTRYAG 1 cut(s) 66
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 225, 367
BstV2I GAAGAC 1 cut(s) 230
BsuRI GGCC 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 546
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 4 cut(s) 155, 279, 333, 428
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 2 cut(s) 331, 630
Cfr13I GGNCC 1 cut(s) 424
CseI GACGC 1 cut(s) 403
Csp6I GTAC 1 cut(s) 201
CviAII CATG 1 cut(s) 418
CviQI GTAC 1 cut(s) 201
DinI GGCGCC 1 cut(s) 305
DpnI GATC 3 cut(s) 51, 87, 594
DpnII GATC 3 cut(s) 49, 85, 592
DriI GACNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 239, 287
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 2 cut(s) 239, 287
Eco57I CTGAAG 1 cut(s) 124
EgeI GGCGCC 1 cut(s) 305
EheI GGCGCC 1 cut(s) 305
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 1 cut(s) 421
FaiI YATR 5 cut(s) 378, 393, 419, 625, 627
FatI CATG 1 cut(s) 417
FauI CCCGC 3 cut(s) 136, 270, 274
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 3 cut(s) 214, 255, 381
Fsp4HI GCNGC 3 cut(s) 214, 255, 381
FspBI CTAG 4 cut(s) 204, 432, 528, 536
GlaI GCGC 1 cut(s) 305
GluI GCNGC 3 cut(s) 214, 255, 381
GsaI CCCAGC 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 1 cut(s) 426
HapII CCGG 3 cut(s) 219, 332, 631
HgaI GACGC 1 cut(s) 403
HhaI GCGC 1 cut(s) 306
Hin1I GRCGYC 2 cut(s) 304, 414
Hin1II CATG 1 cut(s) 421
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 2 cut(s) 62, 645
HpaII CCGG 3 cut(s) 219, 332, 631
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 66, 457
Hpy188I TCNGA 6 cut(s) 49, 90, 143, 150, 374, 650
Hpy188III TCNNGA 4 cut(s) 53, 83, 353, 528
Hpy8I GTNNAC 2 cut(s) 66, 457
Hpy99I CGWCG 3 cut(s) 100, 291, 514
HpyAV CCTTC 3 cut(s) 85, 148, 404
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 5 cut(s) 159, 213, 263, 283, 337
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 2 cut(s) 304, 414
Hsp92II CATG 1 cut(s) 421
HspAI GCGC 1 cut(s) 304
KasI GGCGCC 1 cut(s) 303
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 3 cut(s) 49, 85, 592
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 3 cut(s) 77, 90, 221
LpnPI CCDG 9 cut(s) 54, 167, 232, 345, 392, 440, 501, 591, 644
Lsp1109I GCAGC 2 cut(s) 225, 367
LweI GCATC 1 cut(s) 166
MaeI CTAG 4 cut(s) 204, 432, 528, 536
MaeII ACGT 1 cut(s) 98
MalI GATC 3 cut(s) 51, 87, 594
MboI GATC 3 cut(s) 49, 85, 592
MboII GAAGA 5 cut(s) 235, 241, 256, 304, 461
MhlI GDGCHC 2 cut(s) 95, 251
Mly113I GGCGCC 1 cut(s) 304
MlyI GAGTC 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 331
MspI CCGG 3 cut(s) 219, 332, 631
MspR9I CCNGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 5 cut(s) 159, 213, 263, 283, 337
NaeI GCCGGC 1 cut(s) 333
NarI GGCGCC 1 cut(s) 304
NciI CCSGG 1 cut(s) 220
NdeII GATC 3 cut(s) 49, 85, 592
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 3 cut(s) 73, 305, 325
PaqCI CACCTGC 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PdiI GCCGGC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 645
PfoI TCCNGGA 1 cut(s) 218
PkrI GCNGC 3 cut(s) 215, 256, 382
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 1 cut(s) 70
PluTI GGCGCC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 70
PspFI CCCAGC 1 cut(s) 515
PspN4I GGNNCC 3 cut(s) 73, 305, 325
PspPI GGNCC 1 cut(s) 424
PvuI CGATCG 1 cut(s) 88
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 239
SatI GCNGC 3 cut(s) 214, 255, 381
Sau3AI GATC 3 cut(s) 49, 85, 592
Sau96I GGNCC 1 cut(s) 424
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 220
SduI GDGCHC 2 cut(s) 95, 251
SetI ASST 8 cut(s) 101, 125, 140, 218, 299, 360, 371, 610
SfaNI GCATC 1 cut(s) 166
SfcI CTRYAG 1 cut(s) 66
SfoI GGCGCC 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SsiI CCGC 9 cut(s) 129, 207, 252, 255, 266, 277, 281, 401, 564
SspDI GGCGCC 1 cut(s) 303
SspMI CTAG 4 cut(s) 204, 432, 528, 536
StyD4I CCNGG 1 cut(s) 218
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 101
TaqI TCGA 4 cut(s) 54, 177, 479, 509
TatI WGTACW 1 cut(s) 200
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 1 cut(s) 645
TscAI CASTG 1 cut(s) 553
TseI GCWGC 2 cut(s) 213, 380
TspDTI ATGAA 2 cut(s) 495, 593
TspGWI ACGGA 2 cut(s) 25, 304
TspRI CASTG 1 cut(s) 553
XbaI TCTAGA 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 65
XspI CTAG 4 cut(s) 204, 432, 528, 536
ZrmI AGTACT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.