pycom06g20440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012333
Physical Location & Seq
Forward (+)
23467991 .. 23468670
680 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20440.1

Sequence Viewer

Length: 597 bp
ATGCTAGATCCGAGAAATGACTTGCTGCCGCCGCCCTCCTCCCCCACCAACTCCTCCGTCTCCTCCTCCGATCTCGACACCGAGTCTACAGGGTCGTTCTTTCACGATCGGAGCACGACGTTGGGAACCCTTATGGGAGTGAGTTTCCCCTCCATCACCTTCAGAGCTCCGAGCCAGAACCACCGCGACCCCCAAGCCGGAAACATCAACAACATTGGCAGTACCAGCGGAAGCAGCTCCAGGAAGACGAAGAAGCCGAAAAAGAAGAGTGTCGCCGCTTCGTCGGCGGTGGGTGTGGTGGCGGAGCGGCGGAGGAGGTGGTGGCGGCTTTGCCGTGACGACTGCACCAAGCCGGCTTCGCTCGGGGAGTTTCTTGAGGTGGAGAGGCGGTTCGGAGACGCTGCATTTTATAATACGGCTGCGGAGCTTGAAGGCGTCATGGTGGACCAGCCAAGAAATGGACGGCTGTTGTTCGCCGACGGGAGGGTTCTTCCGCCGCCGAATGTGGATGACGGGATTGCGAATGCATCGACGGCTGGAGTTCTCTCTAGATTGCCGGTTTCACTCACTGCCATCTGTAGTGGCGGTGTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.11

Weight (kDa)

9.21

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17950 AT3G17950 AT3G17950
fragaria_vesca FvH4_5g17460
malus_domestica MD01G1007300.v1.1 MD06G1228000.v1.1 MD14G1237200.v1.1
prunus_persica Prupe.5G234500_v2.0.a1
pyrus_communis pycom06g20440 pycom14g19820
rosa_chinensis RchiOBHm_Chr7g0178231
rosa_multiflora Rmu_sc0007855.1_g000003
rosa_roxburghii Rroxscaffold_3G00275060
rosa_rugosa Rorug06G0415200
rosa_samantha Rh7AG014300 Rh7BG013800 Rh7CG013700 Rh7DG014000
rosa_wichuraiana Rw7G001120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 458
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 86
AccII CGCG 1 cut(s) 186
AclWI GGATC 1 cut(s) 2
AcuI CTGAAG 1 cut(s) 145
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 1 cut(s) 223
AfiI CCNNNNNNNGG 3 cut(s) 197, 458, 483
AgsI TTSAA 1 cut(s) 431
AhdI GACNNNNNGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 239
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 3 cut(s) 167, 237, 427
AluI AGCT 3 cut(s) 167, 237, 427
Alw21I GWGCWC 2 cut(s) 116, 169
Alw26I GTCTC 2 cut(s) 64, 390
AlwI GGATC 1 cut(s) 2
Ama87I CYCGRG 1 cut(s) 362
ApeKI GCWGC 4 cut(s) 25, 234, 401, 419
AspS9I GGNCC 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 1 cut(s) 445
BanII GRGCYC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 116, 169
BbvI GCAGC 4 cut(s) 12, 246, 388, 406
BccI CCATC 2 cut(s) 161, 581
BceAI ACGGC 4 cut(s) 318, 432, 479, 549
BciT130I CCWGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 64, 390
BfaI CTAG 2 cut(s) 5, 549
BfmI CTRYAG 2 cut(s) 87, 577
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 1 cut(s) 445
BmeRI GACNNNNNGTC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 445
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 251
BpmI CTGGAG 2 cut(s) 223, 558
BpuEI CTTGAG 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 458, 483
Bse118I RCCGGY 2 cut(s) 352, 556
BseBI CCWGG 1 cut(s) 241
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 3 cut(s) 197, 458, 483
BseRI GAGGAG 5 cut(s) 28, 43, 52, 55, 328
BseXI GCAGC 4 cut(s) 12, 246, 388, 406
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 2 cut(s) 116, 169
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 3 cut(s) 198, 353, 557
BslI CCNNNNNNNGG 3 cut(s) 197, 458, 483
BsmAI GTCTC 2 cut(s) 64, 390
BsmBI CGTCTC 2 cut(s) 64, 390
BsmI GAATGC 1 cut(s) 529
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 116, 169
Bsp143I GATC 3 cut(s) 7, 70, 106
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 127
BspPI GGATC 1 cut(s) 2
BsrBI CCGCTC 1 cut(s) 307
BsrFI RCCGGY 2 cut(s) 352, 556
BssAI RCCGGY 2 cut(s) 352, 556
BssMI GATC 3 cut(s) 7, 70, 106
BssNI GRCGYC 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 354
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 1 cut(s) 186
BstKTI GATC 3 cut(s) 10, 73, 109
BstMAI GTCTC 2 cut(s) 64, 390
BstMBI GATC 3 cut(s) 7, 70, 106
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 6 cut(s) 31, 225, 234, 284, 358, 533
BstNI CCWGG 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 2 cut(s) 87, 577
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 4 cut(s) 12, 246, 388, 406
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BtsCI GGATG 1 cut(s) 514
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 567
Cac8I GCNNGC 1 cut(s) 354
Cfr10I RCCGGY 2 cut(s) 352, 556
Cfr13I GGNCC 1 cut(s) 445
CseI GACGC 2 cut(s) 407, 424
Csp6I GTAC 1 cut(s) 222
CviAII CATG 1 cut(s) 439
CviQI GTAC 1 cut(s) 222
DpnI GATC 3 cut(s) 9, 72, 108
DpnII GATC 3 cut(s) 7, 70, 106
DriI GACNNNNNGTC 1 cut(s) 82
Eam1104I CTCTTC 1 cut(s) 260
Eam1105I GACNNNNNGTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 3 cut(s) 317, 325, 483
Ecl136II GAGCTC 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 239
EcoT22I ATGCAT 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 169
Esp3I CGTCTC 2 cut(s) 64, 390
FaeI CATG 1 cut(s) 442
FaiI YATR 4 cut(s) 134, 411, 440, 595
FatI CATG 1 cut(s) 438
FblI GTMKAC 1 cut(s) 86
FokI GGATG 1 cut(s) 521
FriOI GRGCYC 1 cut(s) 169
FspBI CTAG 2 cut(s) 5, 549
GsuI CTGGAG 2 cut(s) 223, 558
HapII CCGG 3 cut(s) 198, 353, 557
HgaI GACGC 2 cut(s) 407, 424
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 1 cut(s) 442
HinfI GANTC 1 cut(s) 83
HpaII CCGG 3 cut(s) 198, 353, 557
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 87, 445
Hpy188I TCNGA 6 cut(s) 12, 70, 111, 164, 171, 395
Hpy188III TCNNGA 4 cut(s) 74, 104, 374, 549
Hpy8I GTNNAC 2 cut(s) 87, 445
Hpy99I CGWCG 4 cut(s) 121, 286, 482, 535
HpyAV CCTTC 2 cut(s) 169, 425
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 3 cut(s) 345, 404, 527
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 225, 234, 284, 358, 533
HpySE526I ACGT 1 cut(s) 119
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 1 cut(s) 442
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
Kzo9I GATC 3 cut(s) 7, 70, 106
LmnI GCTCC 5 cut(s) 111, 172, 242, 304, 424
Lsp1109I GCAGC 4 cut(s) 12, 246, 388, 406
LweI GCATC 1 cut(s) 536
MaeI CTAG 2 cut(s) 5, 549
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 335
MalI GATC 3 cut(s) 9, 72, 108
MbiI CCGCTC 1 cut(s) 307
MboI GATC 3 cut(s) 7, 70, 106
MboII GAAGA 4 cut(s) 256, 262, 277, 482
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 116, 169
MlyI GAGTC 1 cut(s) 92
Mph1103I ATGCAT 1 cut(s) 529
MroNI GCCGGC 1 cut(s) 352
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 3 cut(s) 198, 353, 557
MspR9I CCNGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 6 cut(s) 31, 225, 234, 284, 358, 533
NaeI GCCGGC 1 cut(s) 354
NdeII GATC 3 cut(s) 7, 70, 106
NgoMIV GCCGGC 1 cut(s) 352
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 335
NsiI ATGCAT 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 254
PctI GAATGC 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 354
PflMI CCANNNNNTGG 1 cut(s) 458
PfoI TCCNGGA 1 cut(s) 239
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
PsiI TTATAA 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 445
PsuI RGATCY 1 cut(s) 7
PvuI CGATCG 1 cut(s) 109
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SacI GAGCTC 1 cut(s) 169
Sau3AI GATC 3 cut(s) 7, 70, 106
Sau96I GGNCC 1 cut(s) 445
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 2 cut(s) 116, 169
SetI ASST 7 cut(s) 122, 161, 169, 239, 320, 381, 429
SfaNI GCATC 1 cut(s) 536
SfcI CTRYAG 2 cut(s) 87, 577
SinI GGWCC 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SspMI CTAG 2 cut(s) 5, 549
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 239
TaiI ACGT 1 cut(s) 122
TaqI TCGA 2 cut(s) 75, 530
TauI GCSGC 6 cut(s) 31, 34, 278, 310, 328, 499
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 4 cut(s) 25, 234, 401, 419
Tsp45I GTSAC 1 cut(s) 335
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 574
Van91I CCANNNNNTGG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 445
XbaI TCTAGA 1 cut(s) 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 2 cut(s) 5, 549
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.