MD14G1237200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012333
Physical Location & Seq
Forward (+)
31698455 .. 31699296
842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1237200.v1.1.491

Sequence Viewer

Length: 600 bp
ATGCTAGATCCGAGAAATGATATGCTTCCGCCGCCCTCCTCCCCCACCAACTCCTCCGTCTCCTCCTCCGATCTCGACACCGAGTCTACAGGCTCCTTCTTTCACGATCGGAGCACGACGTTAGGAACACTAATGGGAGTGAGCTTCCCCGCCATCACCTTCAGAGCTCCGAGCCAGCACCACCGCGACCCCCAACCCGGAAACATCAACAACATCGGTAGTACTAGCGCAAGCAGCTCCGGGAAGACGAAGAAGCCGAAAAAGAAGAGCGCCGCCGCTTCGTCGGCGGTGGGTGCGCTGGCGGGGCGGCGGAGGAGGTGGTGGCGCCTCTGCCAAGACGACGGCACCAAGCCGGCTTCGCTCGGGGAGTTTCTTGAGGTGGAGAGGAGGTTCGGAGACGCTGCGTTTTACGATACGGCCGCGGAGCTGGAAGGCGTCATGGTGGGCCAGCCCAGAAATGGACGGCTGTTGTTCGCCGACGGGAGGGTTCTTCCGCCGTCGAATGTTGATGATGGGATTGGGACTTCATCGACGGCTGCTGGGGTTCTCTCTAGATTGCCGGTTTCACTCACTGCCATCTGTGGTGGCGGTGTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

20.85

Weight (kDa)

9.09

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17950 AT3G17950 AT3G17950
fragaria_vesca FvH4_5g17460
malus_domestica MD01G1007300.v1.1 MD06G1228000.v1.1 MD14G1237200.v1.1
prunus_persica Prupe.5G234500_v2.0.a1
pyrus_communis pycom06g20440 pycom14g19820
rosa_chinensis RchiOBHm_Chr7g0178231
rosa_multiflora Rmu_sc0007855.1_g000003
rosa_roxburghii Rroxscaffold_3G00275060
rosa_rugosa Rorug06G0415200
rosa_samantha Rh7AG014300 Rh7BG013800 Rh7CG013700 Rh7DG014000
rosa_wichuraiana Rw7G001120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 324, 344
AccI GTMKAC 1 cut(s) 86
AccII CGCG 2 cut(s) 186, 422
AclWI GGATC 1 cut(s) 2
AcoI YGGCCR 1 cut(s) 417
AcuI CTGAAG 1 cut(s) 145
AcyI GRCGYC 2 cut(s) 325, 435
AfaI GTAC 1 cut(s) 223
AfiI CCNNNNNNNGG 3 cut(s) 197, 458, 483
AhdI GACNNNNNGTC 1 cut(s) 82
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 4 cut(s) 144, 167, 237, 427
AluI AGCT 4 cut(s) 144, 167, 237, 427
Alw21I GWGCWC 2 cut(s) 116, 169
Alw26I GTCTC 2 cut(s) 64, 390
AlwI GGATC 1 cut(s) 2
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 2 cut(s) 417, 445
ApeKI GCWGC 3 cut(s) 234, 401, 536
AspLEI GCGC 4 cut(s) 230, 272, 298, 327
AspS9I GGNCC 1 cut(s) 445
AsuC2I CCSGG 2 cut(s) 198, 241
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 362
BanI GGYRCC 2 cut(s) 324, 344
BanII GRGCYC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 116, 169
BbvI GCAGC 3 cut(s) 246, 388, 523
BccI CCATC 3 cut(s) 161, 506, 584
BceAI ACGGC 5 cut(s) 358, 432, 479, 481, 549
BcnI CCSGG 2 cut(s) 198, 241
BcoDI GTCTC 2 cut(s) 64, 390
BfaI CTAG 3 cut(s) 5, 225, 552
BfmI CTRYAG 1 cut(s) 87
BfoI RGCGCY 2 cut(s) 273, 328
BisI GCNGC 8 cut(s) 32, 235, 273, 276, 308, 402, 420, 537
BlsI GCNGC 8 cut(s) 33, 236, 274, 277, 309, 403, 421, 538
BmcAI AGTACT 1 cut(s) 223
Bme1390I CCNGG 2 cut(s) 198, 241
BmeRI GACNNNNNGTC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 445
BmiI GGNNCC 3 cut(s) 94, 326, 346
BmrFI CCNGG 2 cut(s) 198, 241
BpiI GAAGAC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 198, 241
BsaHI GRCGYC 2 cut(s) 325, 435
BsaJI CCNNGG 1 cut(s) 420
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 458, 483
Bse118I RCCGGY 2 cut(s) 352, 559
BseDI CCNNGG 1 cut(s) 420
BseLI CCNNNNNNNGG 3 cut(s) 197, 458, 483
BseRI GAGGAG 6 cut(s) 28, 43, 52, 55, 328, 400
BseX3I CGGCCG 1 cut(s) 417
BseXI GCAGC 3 cut(s) 246, 388, 523
BseYI CCCAGC 1 cut(s) 539
Bsh1236I CGCG 2 cut(s) 186, 422
Bsh1285I CGRYCG 2 cut(s) 109, 420
BshFI GGCC 2 cut(s) 419, 447
BshNI GGYRCC 2 cut(s) 324, 344
BsiEI CGRYCG 2 cut(s) 109, 420
BsiHKAI GWGCWC 2 cut(s) 116, 169
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 4 cut(s) 198, 240, 353, 560
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 3 cut(s) 197, 458, 483
BsmAI GTCTC 2 cut(s) 64, 390
BsmBI CGTCTC 2 cut(s) 64, 390
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 2 cut(s) 419, 447
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 116, 169
Bsp143I GATC 3 cut(s) 7, 70, 106
BspANI GGCC 2 cut(s) 419, 447
BspFNI CGCG 2 cut(s) 186, 422
BspLI GGNNCC 3 cut(s) 94, 326, 346
BspPI GGATC 1 cut(s) 2
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 2 cut(s) 324, 344
BsrFI RCCGGY 2 cut(s) 352, 559
BssAI RCCGGY 2 cut(s) 352, 559
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 3 cut(s) 7, 70, 106
BssNI GRCGYC 2 cut(s) 325, 435
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 2 cut(s) 325, 435
BstC8I GCNNGC 5 cut(s) 176, 232, 300, 354, 449
BstDSI CCRYGG 1 cut(s) 420
BstFNI CGCG 2 cut(s) 186, 422
BstH2I RGCGCY 2 cut(s) 273, 328
BstHHI GCGC 4 cut(s) 230, 272, 298, 327
BstKTI GATC 3 cut(s) 10, 73, 109
BstMAI GTCTC 2 cut(s) 64, 390
BstMBI GATC 3 cut(s) 7, 70, 106
BstMCI CGRYCG 2 cut(s) 109, 420
BstMWI GCNNNNNNNGC 6 cut(s) 31, 234, 284, 293, 304, 358
BstSCI CCNGG 2 cut(s) 196, 239
BstSFI CTRYAG 1 cut(s) 87
BstUI CGCG 2 cut(s) 186, 422
BstV1I GCAGC 3 cut(s) 246, 388, 523
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BstZI CGGCCG 1 cut(s) 417
BsuRI GGCC 2 cut(s) 419, 447
BtgI CCRYGG 1 cut(s) 420
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 5 cut(s) 176, 232, 300, 354, 449
CfoI GCGC 4 cut(s) 230, 272, 298, 327
Cfr10I RCCGGY 2 cut(s) 352, 559
Cfr13I GGNCC 1 cut(s) 445
Cfr42I CCGCGG 1 cut(s) 423
CseI GACGC 2 cut(s) 407, 424
Csp6I GTAC 1 cut(s) 222
CviAII CATG 1 cut(s) 439
CviQI GTAC 1 cut(s) 222
DinI GGCGCC 1 cut(s) 326
DpnI GATC 3 cut(s) 9, 72, 108
DpnII GATC 3 cut(s) 7, 70, 106
DriI GACNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 417
EagI CGGCCG 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 260
Eam1105I GACNNNNNGTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 3 cut(s) 18, 325, 483
Ecl136II GAGCTC 1 cut(s) 167
EclXI CGGCCG 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 169
Eco52I CGGCCG 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 169
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
Esp3I CGTCTC 2 cut(s) 64, 390
FaeI CATG 1 cut(s) 442
FaiI YATR 3 cut(s) 23, 440, 598
FaqI GGGAC 1 cut(s) 535
FatI CATG 1 cut(s) 438
FauI CCCGC 2 cut(s) 157, 295
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 8 cut(s) 32, 235, 273, 276, 308, 402, 420, 537
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 8 cut(s) 32, 235, 273, 276, 308, 402, 420, 537
FspBI CTAG 3 cut(s) 5, 225, 552
GlaI GCGC 4 cut(s) 229, 271, 297, 326
GluI GCNGC 8 cut(s) 32, 235, 273, 276, 308, 402, 420, 537
GsaI CCCAGC 1 cut(s) 543
HaeII RGCGCY 2 cut(s) 273, 328
HaeIII GGCC 2 cut(s) 419, 447
HapII CCGG 4 cut(s) 198, 240, 353, 560
HgaI GACGC 2 cut(s) 407, 424
HhaI GCGC 4 cut(s) 230, 272, 298, 327
Hin1I GRCGYC 2 cut(s) 325, 435
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 4 cut(s) 228, 270, 296, 325
HinP1I GCGC 4 cut(s) 228, 270, 296, 325
HinfI GANTC 1 cut(s) 83
HpaII CCGG 4 cut(s) 198, 240, 353, 560
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 87
Hpy188I TCNGA 6 cut(s) 12, 70, 111, 164, 171, 395
Hpy188III TCNNGA 4 cut(s) 74, 104, 374, 552
Hpy8I GTNNAC 1 cut(s) 87
Hpy99I CGWCG 6 cut(s) 121, 286, 344, 482, 502, 535
HpyAV CCTTC 3 cut(s) 106, 169, 425
HpyCH4IV ACGT 1 cut(s) 119
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 234, 284, 293, 304, 358
HpySE526I ACGT 1 cut(s) 119
Hsp92I GRCGYC 2 cut(s) 325, 435
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 4 cut(s) 228, 270, 296, 325
KasI GGCGCC 1 cut(s) 324
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
KspI CCGCGG 1 cut(s) 423
Kzo9I GATC 3 cut(s) 7, 70, 106
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 5 cut(s) 98, 111, 172, 242, 424
Lsp1109I GCAGC 3 cut(s) 246, 388, 523
MaeI CTAG 3 cut(s) 5, 225, 552
MaeII ACGT 1 cut(s) 119
MalI GATC 3 cut(s) 9, 72, 108
MboI GATC 3 cut(s) 7, 70, 106
MboII GAAGA 4 cut(s) 256, 262, 277, 482
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 116, 169
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 1 cut(s) 92
MroNI GCCGGC 1 cut(s) 352
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 4 cut(s) 198, 240, 353, 560
MspR9I CCNGG 2 cut(s) 198, 241
MvnI CGCG 2 cut(s) 186, 422
MwoI GCNNNNNNNGC 6 cut(s) 31, 234, 284, 293, 304, 358
NaeI GCCGGC 1 cut(s) 354
NarI GGCGCC 1 cut(s) 325
NciI CCSGG 2 cut(s) 198, 241
NdeII GATC 3 cut(s) 7, 70, 106
NgoMIV GCCGGC 1 cut(s) 352
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 3 cut(s) 94, 326, 346
PciSI GCTCTTC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 254
PdiI GCCGGC 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 8 cut(s) 33, 236, 274, 277, 309, 403, 421, 538
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 1 cut(s) 91
PluTI GGCGCC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 539
PspN4I GGNNCC 3 cut(s) 94, 326, 346
PspPI GGNCC 1 cut(s) 445
PsuI RGATCY 1 cut(s) 7
PvuI CGATCG 1 cut(s) 109
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SacI GAGCTC 1 cut(s) 169
SacII CCGCGG 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 260
SatI GCNGC 8 cut(s) 32, 235, 273, 276, 308, 402, 420, 537
Sau3AI GATC 3 cut(s) 7, 70, 106
Sau96I GGNCC 1 cut(s) 445
ScaI AGTACT 1 cut(s) 223
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 198, 241
SduI GDGCHC 2 cut(s) 116, 169
SetI ASST 9 cut(s) 122, 146, 161, 169, 239, 320, 381, 392, 429
SfcI CTRYAG 1 cut(s) 87
SfoI GGCGCC 1 cut(s) 326
Sfr303I CCGCGG 1 cut(s) 423
SgrBI CCGCGG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SspDI GGCGCC 1 cut(s) 324
SspMI CTAG 3 cut(s) 5, 225, 552
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 196, 239
TaiI ACGT 1 cut(s) 122
TaqI TCGA 3 cut(s) 75, 500, 530
TatI WGTACW 1 cut(s) 221
TauI GCSGC 5 cut(s) 34, 275, 278, 310, 422
TscAI CASTG 1 cut(s) 577
TseI GCWGC 3 cut(s) 234, 401, 536
TspDTI ATGAA 1 cut(s) 516
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 577
XbaI TCTAGA 1 cut(s) 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 3 cut(s) 5, 225, 552
ZrmI AGTACT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.