Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008094

Physical Location & Seq

Genomic Coordinates Forward (+)

6515683 .. 6519808

Gene Structure 4126 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1013500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 813 bp

ATGGCATCTAGAACGTCCCAGGCAGCCATTCTCAACCAGCAGCAGCAGCAGCTGGGTTTGTTTGAGAATCAAGAGGGAGAGGGTTGTAATACACAGATGGGTTTCTTTCATTTCCCACCAAACTTGACCTTTTCGTCGTTGCCGTTTTTAGGTCACCAATCTCTCAAAGGCGGCTTCAGTACGGCTAGCATAGCTTCTGATGATGCACCTTCAACCACCACTCTCTCTGAAACCTTAGTCCCCCGGCCATCACTTACTCCTTCCCCTCTAAGGCATAAACAAGATCATAACATCACTACTACTAGTTCTGAATTTGGCGGTGGAGGAGGAGGAGCCCATGCCCATCTCCTTTCTTTGCAAAGATCCACCGCAAATCTCTGGGCATGGGGAGACGTGAGTGATGATCGTGATGAGTATTGCCTGAACAGCAAGAGATCAAGCGGCGGAGACGACCAACATCATCCTTATTTAGGAGGGGTTTCAGCAATGAAGATGAAGAAGATGAAGGCAATTAGGAGGAAGGTGAGGGAGCCTAGGTTTTGCTTCAAAACCCTGAGCGATGTGGATGTGCTTGATGATGGTTACAAGTGGAGAAAGTACGGACAGAAAGTGGTAAAGAACACACAGCATCCAAGGAGTTATTATCGTTGTACGATGGATAACTGCCGCGTAAAGAAACGAGTAGAGCGATTAGCCGAGGACCCGAGAATGGTGATCACAACATATGAAGGGAGACACGTGCATTCTCCATCTCATGATCTTGAGGATCAAGATTCACGATCTCCGTCTCACCTAAACAACTTCTTCTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043455 GO:0043565 GO:0044212 GO:0045595 GO:0045597 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1900376 GO:1901141 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1904368 GO:1904369 GO:1990837 GO:2000112 GO:2000762 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

271

Amino Acids

30.43

Weight (kDa)

8.93

Isoelectric Point (pI)

57.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	192 - 248	1e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G39410
fragaria_vesca	FvH4_1g23082 FvH4_1g23082 FvH4_1g23082 FvH4_1g23082 FvH4_1g23082 FvH4_1g23082 FvH4_1g23082
malus_domestica	MD01G1013500.v1.1 MD15G1337100.v1.1
prunus_persica	Prupe.6G168200_v2.0.a1
pyrus_communis	pycom01g04550 pycom15g28900
rosa_chinensis	RchiOBHm_Chr2g0117781
rosa_laevigata	RLG00000018373
rosa_multiflora	Rmu_sc0005241.1_g000008
rosa_roxburghii	Rroxscaffold_2G00126220
rosa_rugosa	Rorug02G0214100
rosa_samantha	Rh2AG270100 Rh2BG281600 Rh2CG269900 Rh2DG278000 Rh2DG295800
rosa_wichuraiana	Rw2G021340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	133
AccII	CGCG	1 cut(s)	669
AciI	CCGC	6 cut(s)	171, 318, 369, 441, 444, 667
AclWI	GGATC	2 cut(s)	357, 774
AcoI	YGGCCR	1 cut(s)	245
AcsI	RAATTY	1 cut(s)	311
AcuI	CTGAAG	1 cut(s)	160
AcvI	CACGTG	1 cut(s)	739
AfaI	GTAC	3 cut(s)	181, 599, 652
AfiI	CCNNNNNNNGG	4 cut(s)	149, 270, 470, 709
AflIII	ACRYGT	1 cut(s)	736
AgsI	TTSAA	2 cut(s)	213, 547
AhlI	ACTAGT	1 cut(s)	302
AjiI	CACGTC	1 cut(s)	394
AjnI	CCWGG	1 cut(s)	18
AluBI	AGCT	2 cut(s)	52, 194
AluI	AGCT	2 cut(s)	52, 194
Alw26I	GTCTC	4 cut(s)	384, 441, 727, 792
AlwI	GGATC	2 cut(s)	357, 774
AlwNI	CAGNNNCTG	1 cut(s)	52
Ama87I	CYCGRG	1 cut(s)	703
AoxI	GGCC	1 cut(s)	245
ApeKI	GCWGC	5 cut(s)	23, 40, 43, 46, 49
ApoI	RAATTY	1 cut(s)	311
AspA2I	CCTAGG	1 cut(s)	533
AspS9I	GGNCC	1 cut(s)	700
AsuC2I	CCSGG	1 cut(s)	244
AsuHPI	GGTGA	4 cut(s)	146, 535, 724, 782
AsuNHI	GCTAGC	1 cut(s)	185
AvaI	CYCGRG	1 cut(s)	703
AvaII	GGWCC	1 cut(s)	700
AvrII	CCTAGG	1 cut(s)	533
BanII	GRGCYC	1 cut(s)	337
BbrPI	CACGTG	1 cut(s)	739
BbvI	GCAGC	5 cut(s)	35, 52, 55, 58, 61
BccI	CCATC	6 cut(s)	91, 256, 351, 572, 649, 757
BceAI	ACGGC	2 cut(s)	127, 198
BciT130I	CCWGG	1 cut(s)	20
BclI	TGATCA	1 cut(s)	714
BcnI	CCSGG	1 cut(s)	244
BcoDI	GTCTC	4 cut(s)	384, 441, 727, 792
BcuI	ACTAGT	1 cut(s)	302
BfaI	CTAG	4 cut(s)	9, 186, 303, 534
BisI	GCNGC	8 cut(s)	24, 41, 44, 47, 50, 172, 442, 667
BlnI	CCTAGG	1 cut(s)	533
BlsI	GCNGC	8 cut(s)	25, 42, 45, 48, 51, 173, 443, 668
Bme1390I	CCNGG	2 cut(s)	20, 244
Bme18I	GGWCC	1 cut(s)	700
BmeT110I	CYCGRG	1 cut(s)	703
BmgBI	CACGTC	1 cut(s)	394
BmgT120I	GGNCC	1 cut(s)	700
BmiI	GGNNCC	3 cut(s)	334, 531, 702
BmrFI	CCNGG	2 cut(s)	20, 244
BmsI	GCATC	3 cut(s)	14, 193, 637
BmtI	GCTAGC	1 cut(s)	189
Bpu10I	CCTNAGC	1 cut(s)	554
BpuEI	CTTGAG	1 cut(s)	782
BpuMI	CCSGG	1 cut(s)	244
BsaAI	YACGTR	1 cut(s)	739
BsaJI	CCNNGG	5 cut(s)	18, 242, 533, 632, 696
BsaXI	ACNNNNNCTCC	2 cut(s)	521, 551
Bsc4I	CCNNNNNNNGG	4 cut(s)	149, 270, 470, 709
Bse3DI	GCAATG	1 cut(s)	492
BseBI	CCWGG	1 cut(s)	20
BseDI	CCNNGG	5 cut(s)	18, 242, 533, 632, 696
BseGI	GGATG	3 cut(s)	460, 571, 628
BseLI	CCNNNNNNNGG	4 cut(s)	149, 270, 470, 709
BseMI	GCAATG	1 cut(s)	492
BseMII	CTCAG	1 cut(s)	545
BseRI	GAGGAG	3 cut(s)	339, 342, 345
BseXI	GCAGC	5 cut(s)	35, 52, 55, 58, 61
BseYI	CCCAGC	1 cut(s)	52
Bsh1236I	CGCG	1 cut(s)	669
BshFI	GGCC	1 cut(s)	247
BsiHKCI	CYCGRG	1 cut(s)	703
BsiSI	CCGG	1 cut(s)	244
BslFI	GGGAC	1 cut(s)	224
BslI	CCNNNNNNNGG	4 cut(s)	149, 270, 470, 709
BsmAI	GTCTC	4 cut(s)	384, 441, 727, 792
BsmBI	CGTCTC	3 cut(s)	384, 441, 792
BsmFI	GGGAC	1 cut(s)	224
BsmI	GAATGC	1 cut(s)	742
BsnI	GGCC	1 cut(s)	247
BsoBI	CYCGRG	1 cut(s)	703
Bsp1286I	GDGCHC	1 cut(s)	337
Bsp143I	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
BspACI	CCGC	6 cut(s)	171, 318, 369, 441, 444, 667
BspANI	GGCC	1 cut(s)	247
BspCNI	CTCAG	1 cut(s)	546
BspFNI	CGCG	1 cut(s)	669
BspHI	TCATGA	1 cut(s)	754
BspLI	GGNNCC	3 cut(s)	334, 531, 702
BspOI	GCTAGC	1 cut(s)	189
BspPI	GGATC	2 cut(s)	357, 774
BsrDI	GCAATG	1 cut(s)	492
BssECI	CCNNGG	5 cut(s)	18, 242, 533, 632, 696
BssMI	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
BssT1I	CCWWGG	2 cut(s)	533, 632
Bst2UI	CCWGG	1 cut(s)	20
BstBAI	YACGTR	1 cut(s)	739
BstC8I	GCNNGC	1 cut(s)	187
BstDEI	CTNAG	3 cut(s)	235, 269, 554
BstEII	GGTNACC	1 cut(s)	152
BstENI	CCTNNNNNAGG	1 cut(s)	468
BstF5I	GGATG	3 cut(s)	460, 571, 628
BstFNI	CGCG	1 cut(s)	669
BstKTI	GATC	8 cut(s)	286, 365, 406, 437, 717, 760, 769, 782
BstMAI	GTCTC	4 cut(s)	384, 441, 727, 792
BstMBI	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
BstMWI	GCNNNNNNNGC	4 cut(s)	46, 49, 191, 426
BstNI	CCWGG	1 cut(s)	20
BstPI	GGTNACC	1 cut(s)	152
BstSCI	CCNGG	2 cut(s)	18, 242
BstUI	CGCG	1 cut(s)	669
BstV1I	GCAGC	5 cut(s)	35, 52, 55, 58, 61
BstX2I	RGATCY	1 cut(s)	362
BstYI	RGATCY	1 cut(s)	362
BsuRI	GGCC	1 cut(s)	247
BtgZI	GCGATG	1 cut(s)	573
BtrI	CACGTC	1 cut(s)	394
BtsCI	GGATG	3 cut(s)	460, 571, 628
Cac8I	GCNNGC	1 cut(s)	187
CaiI	CAGNNNCTG	1 cut(s)	52
CciI	TCATGA	1 cut(s)	754
Cfr13I	GGNCC	1 cut(s)	700
Csp6I	GTAC	3 cut(s)	180, 598, 651
CspCI	CAANNNNNGTGG	2 cut(s)	105, 140
CviAII	CATG	3 cut(s)	338, 384, 755
CviJI	RGCY	9 cut(s)	26, 52, 174, 185, 194, 247, 335, 532, 695
CviKI_1	RGCY	9 cut(s)	26, 52, 174, 185, 194, 247, 335, 532, 695
CviQI	GTAC	3 cut(s)	180, 598, 651
DdeI	CTNAG	3 cut(s)	235, 269, 554
DpnI	GATC	8 cut(s)	285, 364, 405, 436, 716, 759, 768, 781
DpnII	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
DrdI	GACNNNNNNGTC	1 cut(s)	133
DseDI	GACNNNNNNGTC	1 cut(s)	133
EaeI	YGGCCR	1 cut(s)	245
EciI	GGCGGA	1 cut(s)	459
Eco130I	CCWWGG	2 cut(s)	533, 632
Eco24I	GRGCYC	1 cut(s)	337
Eco47I	GGWCC	1 cut(s)	700
Eco57I	CTGAAG	1 cut(s)	160
Eco72I	CACGTG	1 cut(s)	739
Eco88I	CYCGRG	1 cut(s)	703
Eco91I	GGTNACC	1 cut(s)	152
EcoNI	CCTNNNNNAGG	1 cut(s)	468
EcoO109I	RGGNCCY	1 cut(s)	700
EcoO65I	GGTNACC	1 cut(s)	152
EcoRII	CCWGG	1 cut(s)	18
EcoT14I	CCWWGG	2 cut(s)	533, 632
EcoT38I	GRGCYC	1 cut(s)	337
ErhI	CCWWGG	2 cut(s)	533, 632
Esp3I	CGTCTC	3 cut(s)	384, 441, 792
FaeI	CATG	3 cut(s)	341, 387, 758
FaiI	YATR	8 cut(s)	191, 276, 288, 339, 385, 724, 726, 756
FaqI	GGGAC	1 cut(s)	224
FatI	CATG	3 cut(s)	337, 383, 754
FauNDI	CATATG	1 cut(s)	724
FbaI	TGATCA	1 cut(s)	714
Fnu4HI	GCNGC	8 cut(s)	24, 41, 44, 47, 50, 172, 442, 667
FokI	GGATG	3 cut(s)	447, 578, 615
FriOI	GRGCYC	1 cut(s)	337
Fsp4HI	GCNGC	8 cut(s)	24, 41, 44, 47, 50, 172, 442, 667
FspBI	CTAG	4 cut(s)	9, 186, 303, 534
GluI	GCNGC	8 cut(s)	24, 41, 44, 47, 50, 172, 442, 667
GsaI	CCCAGC	1 cut(s)	56
HaeIII	GGCC	1 cut(s)	247
HapII	CCGG	1 cut(s)	244
Hin1II	CATG	3 cut(s)	341, 387, 758
HinfI	GANTC	2 cut(s)	67, 773
HpaII	CCGG	1 cut(s)	244
HphI	GGTGA	4 cut(s)	146, 535, 724, 782
Hpy188I	TCNGA	3 cut(s)	199, 229, 310
Hpy188III	TCNNGA	7 cut(s)	9, 71, 407, 755, 761, 770, 777
Hpy99I	CGWCG	1 cut(s)	139
HpyAV	CCTTC	5 cut(s)	219, 270, 499, 514, 722
HpyCH4IV	ACGT	3 cut(s)	14, 393, 738
HpyCH4V	TGCA	3 cut(s)	206, 358, 742
HpyF10VI	GCNNNNNNNGC	4 cut(s)	46, 49, 191, 426
HpyF3I	CTNAG	3 cut(s)	235, 269, 554
HpySE526I	ACGT	3 cut(s)	14, 393, 738
Hsp92II	CATG	3 cut(s)	341, 387, 758
Ksp22I	TGATCA	1 cut(s)	714
Kzo9I	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
LmnI	GCTCC	2 cut(s)	332, 529
LpnPI	CCDG	9 cut(s)	5, 32, 38, 50, 257, 364, 434, 566, 793
Lsp1109I	GCAGC	5 cut(s)	35, 52, 55, 58, 61
LweI	GCATC	3 cut(s)	14, 193, 637
MaeI	CTAG	4 cut(s)	9, 186, 303, 534
MaeII	ACGT	3 cut(s)	14, 393, 738
MaeIII	GTNAC	2 cut(s)	152, 581
MalI	GATC	8 cut(s)	285, 364, 405, 436, 716, 759, 768, 781
MboI	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
MboII	GAAGA	4 cut(s)	502, 508, 511, 796
MflI	RGATCY	1 cut(s)	362
MhlI	GDGCHC	1 cut(s)	337
MluCI	AATT	2 cut(s)	311, 510
MspA1I	CMGCKG	1 cut(s)	52
MspI	CCGG	1 cut(s)	244
MspR9I	CCNGG	2 cut(s)	20, 244
Mva1269I	GAATGC	1 cut(s)	742
MvaI	CCWGG	1 cut(s)	20
MvnI	CGCG	1 cut(s)	669
MwoI	GCNNNNNNNGC	4 cut(s)	46, 49, 191, 426
NciI	CCSGG	1 cut(s)	244
NdeI	CATATG	1 cut(s)	724
NdeII	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
NheI	GCTAGC	1 cut(s)	185
NlaIII	CATG	3 cut(s)	341, 387, 758
NlaIV	GGNNCC	3 cut(s)	334, 531, 702
NmeAIII	GCCGAG	1 cut(s)	721
NmuCI	GTSAC	1 cut(s)	152
PagI	TCATGA	1 cut(s)	754
PcsI	WCGNNNNNNNCGW	2 cut(s)	140, 685
PctI	GAATGC	1 cut(s)	742
PfeI	GAWTC	2 cut(s)	67, 773
PkrI	GCNGC	8 cut(s)	25, 42, 45, 48, 51, 173, 443, 668
PmaCI	CACGTG	1 cut(s)	739
PmlI	CACGTG	1 cut(s)	739
Ppu21I	YACGTR	1 cut(s)	739
PpuMI	RGGWCCY	1 cut(s)	700
Psp5II	RGGWCCY	1 cut(s)	700
Psp6I	CCWGG	1 cut(s)	18
PspCI	CACGTG	1 cut(s)	739
PspEI	GGTNACC	1 cut(s)	152
PspFI	CCCAGC	1 cut(s)	52
PspGI	CCWGG	1 cut(s)	18
PspN4I	GGNNCC	3 cut(s)	334, 531, 702
PspPI	GGNCC	1 cut(s)	700
PspPPI	RGGWCCY	1 cut(s)	700
PstNI	CAGNNNCTG	1 cut(s)	52
PsuI	RGATCY	1 cut(s)	362
PvuII	CAGCTG	1 cut(s)	52
RsaI	GTAC	3 cut(s)	181, 599, 652
RsaNI	GTAC	3 cut(s)	180, 598, 651
SatI	GCNGC	8 cut(s)	24, 41, 44, 47, 50, 172, 442, 667
Sau3AI	GATC	8 cut(s)	283, 362, 403, 434, 714, 757, 766, 779
Sau96I	GGNCC	1 cut(s)	700
ScrFI	CCNGG	2 cut(s)	20, 244
SduI	GDGCHC	1 cut(s)	337
SfaNI	GCATC	3 cut(s)	14, 193, 637
SinI	GGWCC	1 cut(s)	700
SmlI	CTYRAG	1 cut(s)	761
SmoI	CTYRAG	1 cut(s)	761
SpeI	ACTAGT	1 cut(s)	302
Sse9I	AATT	2 cut(s)	311, 510
SsiI	CCGC	6 cut(s)	171, 318, 369, 441, 444, 667
SspMI	CTAG	4 cut(s)	9, 186, 303, 534
StyD4I	CCNGG	2 cut(s)	18, 242
StyI	CCWWGG	2 cut(s)	533, 632
TaiI	ACGT	3 cut(s)	17, 396, 741
TasI	AATT	2 cut(s)	311, 510
TauI	GCSGC	3 cut(s)	174, 444, 669
TfiI	GAWTC	2 cut(s)	67, 773
TseFI	GTSAC	1 cut(s)	152
TseI	GCWGC	5 cut(s)	23, 40, 43, 46, 49
Tsp45I	GTSAC	1 cut(s)	152
TspDTI	ATGAA	5 cut(s)	98, 503, 509, 518, 741
TspGWI	ACGGA	2 cut(s)	615, 774
VpaK11BI	GGWCC	1 cut(s)	700
XagI	CCTNNNNNAGG	1 cut(s)	468
XapI	RAATTY	1 cut(s)	311
XbaI	TCTAGA	1 cut(s)	8
XmaJI	CCTAGG	1 cut(s)	533
XspI	CTAG	4 cut(s)	9, 186, 303, 534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.