Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004432

Physical Location & Seq

Genomic Coordinates Reverse (-)

9335555 .. 9339061

Gene Structure 3507 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1024900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGGACCATGACGACTTTCGGACCATCTTAGAAAGTTCGGAAGTGGATATATGGACGTTCATGGACACTGCCATAGCTGTCGCTTCGGCTGATTATGGCACTGAGCTCAAGCACCGGAGGGATGGCATTGTCGAGCGCCTCTATGCCACAACTTCCGTTCCGCCTCAATGCCCAAACTGCGATGCTGATGGGCTTAATGGGCACAATGGTAACCAGCCCAAAGCAGTTTCACCGTACACTCCGCAGTCTGTTGGTGGAGATGGTGGTGAGGAGGATGAGGAGGAGTTGGATCCCTACGCAGGGTTGTTCGATGATGAGCAGAAGAAGATTCTAGACATTAAGAAGCAGCTTGAGGATCCCTATCAGTCTGAGGATACATTGGTTGAGTTGCTTCAAACTCTAGCAGATATGGAAATATCAGTCCAAGAACTTAAGGAGGCTGATATTGGAAAGCATGTGAGTCAATTGCGGAAGCAGCATCCAAGCGATGTTGTTAGGCGATTGGCAAAGCAAATTGTCAATAAATGGAAAGGTATTGTGAATGAATGGGTCAAGTTACATGGAGAACAACAATCATCTTCAACCATGGTTGATGAAGACTCACCACAGCAGAAAATTCCCCACAATGGACTTCACCAGGTTCCTGATTTTGCATACTCTCCAAATCCTCACAATGGGAGTTCTGGGTCAGACAAGAACAATTCAGAACCAGAACGCAAGTCAAAGGCAGTTCCTAGAAGGGATGCTCCACCAAAACCTACACAATTGACCCCTGTGTCTGCTTCTGCTCCTCAAAATAGACAGATGGGGCAGAAGGAAAGCAATTTTGACTCTGATAGATTGGCTTCTGCCAGAAGACGACTTCAGGCAAATTACAAAGAAGCTGAAAATGCCAAAAAGCAGAGAACAATTCAAGTGATGGACATCCATGAGATACCAAAGCCGAAGAATTCTTTCATTGCGAGGAACAAAGGCGGCGGAGGAGGCGGAAGTGGCTCTCATCAAGGCAGGCATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006354 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0070449 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med26

Protein Analysis

340

Amino Acids

37.73

Weight (kDa)

5.68

Isoelectric Point (pI)

54.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med26	PF08711	129 - 180	1.1e-13	TFIIS helical bundle-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004432)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09850 AT5G09850 AT5G09850 AT5G09850 AT5G09850
fragaria_vesca	FvH4_1g23670 FvH4_1g23670 FvH4_1g23670 FvH4_1g23670
malus_domestica	MD01G1024900.v1.1 MD15G1319400.v1.1
prunus_persica	Prupe.6G180300_v2.0.a1 Prupe.6G180300_v2.0.a1 Prupe.6G180300_v2.0.a1 Prupe.6G180300_v2.0.a1 Prupe.6G180300_v2.0.a1 Prupe.6G180300_v2.0.a1
pyrus_communis	pycom01g05280 pycom15g29740
rosa_chinensis	RchiOBHm_Chr2g0119271
rosa_laevigata	RLG00000018461
rosa_multiflora	Rmu_co8400383.1_g000001 Rmu_sc0002788.1_g000002 Rmu_ssc0000264.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124360
rosa_rugosa	Rorug02G0221400 Rorug02G0221400
rosa_samantha	Rh2AG279500 Rh2BG290200 Rh2DG285500 Rh2DG304500
rosa_wichuraiana	Rw2G022230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	775
AciI	CCGC	6 cut(s)	161, 242, 469, 975, 978, 987
AclWI	GGATC	4 cut(s)	284, 297, 350, 363
AcsI	RAATTY	2 cut(s)	615, 949
AcuI	CTGAAG	1 cut(s)	848
AfaI	GTAC	1 cut(s)	236
AfiI	CCNNNNNNNGG	4 cut(s)	299, 300, 626, 674
AflII	CTTAAG	1 cut(s)	431
AgsI	TTSAA	3 cut(s)	395, 582, 914
AjnI	CCWGG	1 cut(s)	636
AluBI	AGCT	4 cut(s)	77, 106, 349, 884
AluI	AGCT	4 cut(s)	77, 106, 349, 884
Alw21I	GWGCWC	1 cut(s)	108
AlwI	GGATC	4 cut(s)	284, 297, 350, 363
ApeKI	GCWGC	2 cut(s)	346, 475
ApoI	RAATTY	2 cut(s)	615, 949
Asp700I	GAANNNNTTC	1 cut(s)	953
AspLEI	GCGC	1 cut(s)	138
AspS9I	GGNCC	2 cut(s)	4, 21
AsuHPI	GGTGA	4 cut(s)	222, 278, 594, 626
AvaII	GGWCC	2 cut(s)	4, 21
BaeGI	GKGCMC	1 cut(s)	204
BaeI	ACNNNNGTAYC	2 cut(s)	366, 399
BamHI	GGATCC	2 cut(s)	289, 355
BanII	GRGCYC	1 cut(s)	108
BbsI	GAAGAC	2 cut(s)	603, 862
Bbv12I	GWGCWC	1 cut(s)	108
BbvI	GCAGC	2 cut(s)	358, 487
BccI	CCATC	6 cut(s)	32, 116, 182, 254, 799, 913
BciT130I	CCWGG	1 cut(s)	638
BciVI	GTATCC	1 cut(s)	367
BfaI	CTAG	3 cut(s)	332, 401, 735
BfoI	RGCGCY	1 cut(s)	139
BfrI	CTTAAG	1 cut(s)	431
BfuI	GTATCC	1 cut(s)	367
BisI	GCNGC	3 cut(s)	347, 476, 976
BlsI	GCNGC	3 cut(s)	348, 477, 977
Bme1390I	CCNGG	1 cut(s)	638
Bme18I	GGWCC	2 cut(s)	4, 21
BmgT120I	GGNCC	2 cut(s)	4, 21
BmiI	GGNNCC	3 cut(s)	291, 357, 642
BmrFI	CCNGG	1 cut(s)	638
BmsI	GCATC	3 cut(s)	172, 487, 733
BpiI	GAAGAC	2 cut(s)	603, 862
BpuEI	CTTGAG	2 cut(s)	92, 371
BsaBI	GATNNNNATC	2 cut(s)	360, 923
BsaJI	CCNNGG	1 cut(s)	585
BsaWI	WCCGGW	1 cut(s)	114
Bsc4I	CCNNNNNNNGG	4 cut(s)	299, 300, 626, 674
Bse3DI	GCAATG	1 cut(s)	957
Bse8I	GATNNNNATC	2 cut(s)	360, 923
BseBI	CCWGG	1 cut(s)	638
BseDI	CCNNGG	1 cut(s)	585
BseGI	GGATG	5 cut(s)	127, 280, 478, 748, 924
BseJI	GATNNNNATC	2 cut(s)	360, 923
BseLI	CCNNNNNNNGG	4 cut(s)	299, 300, 626, 674
BseMI	GCAATG	1 cut(s)	957
BseMII	CTCAG	2 cut(s)	93, 360
BseRI	GAGGAG	5 cut(s)	284, 293, 296, 780, 996
BseSI	GKGCMC	1 cut(s)	204
BseXI	GCAGC	2 cut(s)	358, 487
BsiHKAI	GWGCWC	1 cut(s)	108
BsiSI	CCGG	1 cut(s)	115
BslI	CCNNNNNNNGG	4 cut(s)	299, 300, 626, 674
Bsp1286I	GDGCHC	2 cut(s)	108, 204
Bsp143I	GATC	2 cut(s)	289, 355
Bsp19I	CCATGG	1 cut(s)	585
BspACI	CCGC	6 cut(s)	161, 242, 469, 975, 978, 987
BspCNI	CTCAG	2 cut(s)	94, 361
BspLI	GGNNCC	3 cut(s)	291, 357, 642
BspPI	GGATC	4 cut(s)	284, 297, 350, 363
BspTI	CTTAAG	1 cut(s)	431
BsrDI	GCAATG	1 cut(s)	957
BssECI	CCNNGG	1 cut(s)	585
BssMI	GATC	2 cut(s)	289, 355
BssT1I	CCWWGG	1 cut(s)	585
Bst2UI	CCWGG	1 cut(s)	638
Bst4CI	ACNGT	1 cut(s)	234
BstAFI	CTTAAG	1 cut(s)	431
BstC8I	GCNNGC	1 cut(s)	1010
BstDEI	CTNAG	3 cut(s)	28, 102, 369
BstDSI	CCRYGG	1 cut(s)	585
BstEII	GGTNACC	1 cut(s)	209
BstF5I	GGATG	5 cut(s)	127, 280, 478, 748, 924
BstH2I	RGCGCY	1 cut(s)	139
BstHHI	GCGC	1 cut(s)	138
BstKTI	GATC	2 cut(s)	292, 358
BstMBI	GATC	2 cut(s)	289, 355
BstMWI	GCNNNNNNNGC	6 cut(s)	177, 199, 475, 890, 984, 993
BstNI	CCWGG	1 cut(s)	638
BstNSI	RCATGY	1 cut(s)	458
BstPI	GGTNACC	1 cut(s)	209
BstSCI	CCNGG	1 cut(s)	636
BstSLI	GKGCMC	1 cut(s)	204
BstV1I	GCAGC	2 cut(s)	358, 487
BstV2I	GAAGAC	2 cut(s)	603, 862
BstX2I	RGATCY	2 cut(s)	289, 355
BstYI	RGATCY	2 cut(s)	289, 355
BsuI	GTATCC	1 cut(s)	367
BtgI	CCRYGG	1 cut(s)	585
BtgZI	GCGATG	2 cut(s)	195, 501
BtsCI	GGATG	5 cut(s)	127, 280, 478, 748, 924
BtsI	GCAGTG	1 cut(s)	66
BtsIMutI	CAGTG	2 cut(s)	66, 99
Cac8I	GCNNGC	1 cut(s)	1010
CfoI	GCGC	1 cut(s)	138
Cfr13I	GGNCC	2 cut(s)	4, 21
CsiI	ACCWGGT	1 cut(s)	636
Csp6I	GTAC	1 cut(s)	235
CviAII	CATG	6 cut(s)	8, 61, 455, 560, 586, 929
CviQI	GTAC	1 cut(s)	235
DdeI	CTNAG	3 cut(s)	28, 102, 369
DpnI	GATC	2 cut(s)	291, 357
DpnII	GATC	2 cut(s)	289, 355
DrdI	GACNNNNNNGTC	1 cut(s)	775
DseDI	GACNNNNNNGTC	1 cut(s)	775
EciI	GGCGGA	3 cut(s)	150, 993, 1002
Ecl136II	GAGCTC	1 cut(s)	106
Eco130I	CCWWGG	1 cut(s)	585
Eco24I	GRGCYC	1 cut(s)	108
Eco47I	GGWCC	2 cut(s)	4, 21
Eco53kI	GAGCTC	1 cut(s)	106
Eco57I	CTGAAG	1 cut(s)	848
Eco91I	GGTNACC	1 cut(s)	209
EcoICRI	GAGCTC	1 cut(s)	106
EcoO65I	GGTNACC	1 cut(s)	209
EcoRI	GAATTC	1 cut(s)	949
EcoRII	CCWGG	1 cut(s)	636
EcoT14I	CCWWGG	1 cut(s)	585
EcoT38I	GRGCYC	1 cut(s)	108
ErhI	CCWWGG	1 cut(s)	585
FaeI	CATG	6 cut(s)	11, 64, 458, 563, 589, 932
FatI	CATG	6 cut(s)	7, 60, 454, 559, 585, 928
Fnu4HI	GCNGC	3 cut(s)	347, 476, 976
FokI	GGATG	5 cut(s)	134, 287, 465, 755, 911
FriOI	GRGCYC	1 cut(s)	108
Fsp4HI	GCNGC	3 cut(s)	347, 476, 976
FspBI	CTAG	3 cut(s)	332, 401, 735
GlaI	GCGC	1 cut(s)	137
GluI	GCNGC	3 cut(s)	347, 476, 976
HaeII	RGCGCY	1 cut(s)	139
HapII	CCGG	1 cut(s)	115
HhaI	GCGC	1 cut(s)	138
Hin1II	CATG	6 cut(s)	11, 64, 458, 563, 589, 932
Hin6I	GCGC	1 cut(s)	136
HinP1I	GCGC	1 cut(s)	136
HinfI	GANTC	4 cut(s)	328, 460, 599, 830
HpaII	CCGG	1 cut(s)	115
HphI	GGTGA	4 cut(s)	222, 278, 594, 626
Hpy166II	GTNNAC	1 cut(s)	237
Hpy188I	TCNGA	6 cut(s)	21, 40, 370, 691, 706, 835
Hpy188III	TCNNGA	2 cut(s)	332, 644
Hpy8I	GTNNAC	1 cut(s)	237
HpyAV	CCTTC	2 cut(s)	732, 808
HpyCH4III	ACNGT	1 cut(s)	234
HpyCH4IV	ACGT	1 cut(s)	56
HpyCH4V	TGCA	1 cut(s)	653
HpyF10VI	GCNNNNNNNGC	6 cut(s)	177, 199, 475, 890, 984, 993
HpyF3I	CTNAG	3 cut(s)	28, 102, 369
HpySE526I	ACGT	1 cut(s)	56
Hsp92II	CATG	6 cut(s)	11, 64, 458, 563, 589, 932
HspAI	GCGC	1 cut(s)	136
Kzo9I	GATC	2 cut(s)	289, 355
LmnI	GCTCC	2 cut(s)	751, 793
Lsp1109I	GCAGC	2 cut(s)	358, 487
LweI	GCATC	3 cut(s)	172, 487, 733
MabI	ACCWGGT	1 cut(s)	636
MaeI	CTAG	3 cut(s)	332, 401, 735
MaeII	ACGT	1 cut(s)	56
MaeIII	GTNAC	2 cut(s)	209, 555
MalI	GATC	2 cut(s)	291, 357
MboI	GATC	2 cut(s)	289, 355
MboII	GAAGA	6 cut(s)	334, 337, 570, 608, 867, 958
MfeI	CAATTG	2 cut(s)	464, 764
MflI	RGATCY	2 cut(s)	289, 355
MhlI	GDGCHC	2 cut(s)	108, 204
MluCI	AATT	9 cut(s)	464, 513, 615, 700, 764, 823, 871, 909, 949
MlyI	GAGTC	3 cut(s)	469, 593, 824
MmeI	TCCRAC	1 cut(s)	267
MroXI	GAANNNNTTC	1 cut(s)	953
MseI	TTAA	3 cut(s)	195, 339, 432
MspCI	CTTAAG	1 cut(s)	431
MspI	CCGG	1 cut(s)	115
MspR9I	CCNGG	1 cut(s)	638
MunI	CAATTG	2 cut(s)	464, 764
MvaI	CCWGG	1 cut(s)	638
MwoI	GCNNNNNNNGC	6 cut(s)	177, 199, 475, 890, 984, 993
NcoI	CCATGG	1 cut(s)	585
NdeII	GATC	2 cut(s)	289, 355
NlaIII	CATG	6 cut(s)	11, 64, 458, 563, 589, 932
NlaIV	GGNNCC	3 cut(s)	291, 357, 642
NspI	RCATGY	1 cut(s)	458
PdmI	GAANNNNTTC	1 cut(s)	953
PfeI	GAWTC	1 cut(s)	328
PkrI	GCNGC	3 cut(s)	348, 477, 977
PleI	GAGTC	3 cut(s)	468, 593, 824
PpsI	GAGTC	3 cut(s)	468, 593, 824
Psp124BI	GAGCTC	1 cut(s)	108
Psp6I	CCWGG	1 cut(s)	636
PspEI	GGTNACC	1 cut(s)	209
PspGI	CCWGG	1 cut(s)	636
PspN4I	GGNNCC	3 cut(s)	291, 357, 642
PspPI	GGNCC	2 cut(s)	4, 21
PsuI	RGATCY	2 cut(s)	289, 355
RsaI	GTAC	1 cut(s)	236
RsaNI	GTAC	1 cut(s)	235
SacI	GAGCTC	1 cut(s)	108
SaqAI	TTAA	3 cut(s)	195, 339, 432
SatI	GCNGC	3 cut(s)	347, 476, 976
Sau3AI	GATC	2 cut(s)	289, 355
Sau96I	GGNCC	2 cut(s)	4, 21
SchI	GAGTC	3 cut(s)	469, 593, 824
ScrFI	CCNGG	1 cut(s)	638
SduI	GDGCHC	2 cut(s)	108, 204
SetI	ASST	8 cut(s)	59, 79, 108, 351, 535, 642, 760, 886
SexAI	ACCWGGT	1 cut(s)	636
SfaNI	GCATC	3 cut(s)	172, 487, 733
SinI	GGWCC	2 cut(s)	4, 21
SmlI	CTYRAG	3 cut(s)	107, 350, 431
SmoI	CTYRAG	3 cut(s)	107, 350, 431
Sse9I	AATT	9 cut(s)	464, 513, 615, 700, 764, 823, 871, 909, 949
SsiI	CCGC	6 cut(s)	161, 242, 469, 975, 978, 987
SspMI	CTAG	3 cut(s)	332, 401, 735
SstI	GAGCTC	1 cut(s)	108
StyD4I	CCNGG	1 cut(s)	636
StyI	CCWWGG	1 cut(s)	585
TaaI	ACNGT	1 cut(s)	234
TaiI	ACGT	1 cut(s)	59
TaqI	TCGA	2 cut(s)	132, 309
TasI	AATT	9 cut(s)	464, 513, 615, 700, 764, 823, 871, 909, 949
TauI	GCSGC	1 cut(s)	978
TfiI	GAWTC	1 cut(s)	328
Tru1I	TTAA	3 cut(s)	195, 339, 432
Tru9I	TTAA	3 cut(s)	195, 339, 432
TscAI	CASTG	2 cut(s)	73, 106
TseI	GCWGC	2 cut(s)	346, 475
TspDTI	ATGAA	4 cut(s)	49, 558, 609, 946
TspGWI	ACGGA	1 cut(s)	145
TspRI	CASTG	2 cut(s)	73, 106
Vha464I	CTTAAG	1 cut(s)	431
VpaK11BI	GGWCC	2 cut(s)	4, 21
XapI	RAATTY	2 cut(s)	615, 949
XbaI	TCTAGA	1 cut(s)	331
XceI	RCATGY	1 cut(s)	458
XmnI	GAANNNNTTC	1 cut(s)	953
XspI	CTAG	3 cut(s)	332, 401, 735

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.