Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012504

Physical Location & Seq

Genomic Coordinates Reverse (-)

9875931 .. 9880929

Gene Structure 4999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1026400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGCAAAGCCCAGATCCAACGGCGGAGGACGAATCCCAGGCCGTCAGCCGCCGCATCCAGCGGTTGTCCCTGCACCTGACCCCCAAGTACCCACTTCCCCGAAACTACGCAGCTCAGCAGCTTGAGCTGGTGGAGTGCGCTTCCAAGGCGGCGAAGCTGACGGTTGACACGAGCAGCCTCTCAAACTACATGAGAGGCAAGCACCGGGAAATCCAGGACAGAGTGTTGGATTACTTCAACAAGCAGCCGGAGCTTCAGACGCCGGTGGAGATTTTGAAGGACGACCACCGGGAGCTTTGCATGAAGCAGCTTGTGGGGTTGGTGAAAGAGGCTGGGATCAGGCCTTTCCGGTACGTCGTCGATGACCCGGCCAAGTACTTTGCTATTTTGGAGGCCGTTGGCAGCGTCGACATGTCTCTCGGGATTAAGATGGGGGTGCAGTACAGTCTTTGGGGAGGTTCTGTACTTAACTTAGGAACCAAAAAGCACAAGGATAAGTATTTTGATGGTATCGACAATATGGAGTATCCAGGTTGTTTTGCTATGACTGAACTACATCATGGATCAAATGTTCAAGGTCTACAAACCGAGGCAACCTTTGATCCACTCACAGATGATTTTATAATCGACACACCCAATGATGGGGCCATCAAATGGTGGATTGGCAATGCTGCCGTTCATGGAAAGTTTGCTACTGTTTTCGCTAAGCTGATGTTGCCGACTCATGACACAAAAGGTATTACTGATATGGGTGTCCATGCCTTCATTGTGCCAATAAGGGATTTCAAGACCCACCAAACTCTTCCAGGAATTGAGATACATGATTGTGGCCACAAGGTTGGTCTGAATGGGGTAGACAATGGAGCATTAAGATTCTGCTCAGTGAGAATCCCTCGTGATAATCTTCTTAATCGGTTTGGAGATGTCTCCCGGGACGGGAAATACACAAGTAGTTTACCATCTATAAATAAAAGATTTGCTGCCACTCTAGGTGAACTTGTAGGTGGGAGGGTCGGTCTTGCATATTCTTCAGTTAGTGTCCTCAAGATCGCTGCCACAATTGCAATCCGATATTCTCTACTGCGCCAGCAGTTTGGTCCTCCCAAGCAACCTGAAGTTAGTATTCTTGATTATCAGTCTCAACAACACAAGTTAATGCCCATGCTGGCTTCAACTTATGCATTCCATTTTGCCACGTTGCATTTGGTGGAGAAATATTCAGAGATGAAAAAGTCTCATGATGAACAATTGGTTGGTGACGTCCATTCGCTTTCAGCAGGGCTCAAGGCTTATGTGACAGCATATACGGCAAAGTCATTAAGTATCTGCAGGGAATCTTGTGGAGGCCATGGGTATGCTGCTGTCAACCGCTTCGGTAGCCTGAGAAATGACCATGACATTTTTCAGACGTTTGAAGGGGACAATACAGTGCTTCTACAACAGGTTGCGGGTGATCTGTTGAAGCAATATAAGGAGAAATTTCAAGGCGGGACACTCACTGTCACATGGAACTACCTCAGAGAGTCCATGAACTCGTATCTATCTCAGCCAAATCCAGTGACTGCTCGATGGGAAAGCGAAGACCATTTGCGAGATCCTAAGTTCCAGTTGGATGCTTTCAGATACCGAACTTCTCGATTACTTCAAAGTGTTGCAGTAAGACTGCGCAAGCATTCCAAAACTCTTGGCAGTTTTGGTGCATGGAATCAATGCTTAAATCACCTTTTGACTCTGGCGGAGTCCCATATTGAGTCAGTCATCCTTGCCAAGTTTGTTGAAGCTGTCCAGAAATGTCCGGATCCAAGTTCTCGAGCTGCTCTGAAACTCGTCTGTGATCTTTATGCATTGGAGCGCATTTGGAAAGACATAGGAACCTATCGTAATGTTGATTATGTTGCACCAAACAAAGCTAAGGCAATTCACAAACTGATGGAGTACCTGAGTTTCCAGGTGAGAAATATAGCAAGGGAACTTGTAGACACATTTGACATTCCAGATTATGTCACAAGAGCCCCAATTGCAATGCAGTCGGATGCCTACTCACACTACACACACTACGTCGGATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042579 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071704 GO:0072329 GO:0097159 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_1 Acyl-CoA_dh_M

Protein Analysis

690

Amino Acids

77.53

Weight (kDa)

8.44

Isoelectric Point (pI)

39.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_M	PF02770	179 - 293	8.3e-12	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	328 - 484	3.4e-09	Acyl-CoA dehydrogenase, C-terminal domain
ACOX_C_alpha1	PF22924	331 - 486	4.8e-50	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	532 - 683	6.1e-25	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65110 AT5G65110
fragaria_vesca	FvH4_1g21250
malus_domestica	MD01G1026400.v1.1 MD15G1327100.v1.1
prunus_persica	Prupe.6G181800_v2.0.a1
pyrus_communis	pycom01g05460 pycom395g00040
rosa_chinensis	RchiOBHm_Chr2g0115091
rosa_laevigata	RLG00000018156
rosa_multiflora	Rmu_sc0002784.1_g000002
rosa_roxburghii	Rroxscaffold_2G00130560
rosa_rugosa	Rorug02G0193300 Rorug02G0193400
rosa_samantha	Rh2AG251800 Rh2BG261400 Rh2CG256300 Rh2DG259900
rosa_wichuraiana	Rw2G019640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	623
AatII	GACGTC	1 cut(s)	1263
Acc16I	TGCGCA	1 cut(s)	1667
AccB7I	CCANNNNNTGG	1 cut(s)	654
AccI	GTMKAC	4 cut(s)	408, 580, 855, 1977
AccIII	TCCGGA	1 cut(s)	1795
AciI	CCGC	9 cut(s)	23, 49, 52, 61, 149, 1369, 1448, 1488, 1736
AclWI	GGATC	7 cut(s)	8, 344, 571, 596, 1589, 1793, 1806
AcoI	YGGCCR	2 cut(s)	369, 829
AcsI	RAATTY	1 cut(s)	1478
AcuI	CTGAAG	3 cut(s)	239, 1014, 1134
AcyI	GRCGYC	2 cut(s)	260, 1260
AfaI	GTAC	6 cut(s)	89, 353, 377, 443, 465, 1937
AfiI	CCNNNNNNNGG	4 cut(s)	641, 642, 654, 936
AflIII	ACRYGT	1 cut(s)	411
AjnI	CCWGG	5 cut(s)	36, 213, 529, 805, 1947
AjuI	GAANNNNNNNTTGG	2 cut(s)	1236, 1268
AloI	GAACNNNNNNTCC	2 cut(s)	555, 587
Alw26I	GTCTC	4 cut(s)	420, 931, 1143, 1239
AlwI	GGATC	7 cut(s)	8, 344, 571, 596, 1589, 1793, 1806
AlwNI	CAGNNNCTG	1 cut(s)	1562
Ama87I	CYCGRG	3 cut(s)	419, 930, 1809
Aor13HI	TCCGGA	1 cut(s)	1795
AoxI	GGCC	7 cut(s)	39, 341, 369, 393, 645, 829, 1345
ApoI	RAATTY	1 cut(s)	1478
AspLEI	GCGC	4 cut(s)	140, 1086, 1668, 1854
AspS9I	GGNCC	2 cut(s)	645, 1097
AsuC2I	CCSGG	5 cut(s)	206, 290, 368, 931, 932
AsuHPI	GGTGA	6 cut(s)	334, 1004, 1268, 1463, 1712, 1963
AvaI	CYCGRG	3 cut(s)	419, 930, 1809
AvaII	GGWCC	1 cut(s)	1097
BalI	TGGCCA	1 cut(s)	831
BamHI	GGATCC	1 cut(s)	1798
BanII	GRGCYC	2 cut(s)	1284, 2014
BauI	CACGAG	2 cut(s)	169, 894
BbsI	GAAGAC	1 cut(s)	1587
BccI	CCATC	7 cut(s)	424, 500, 635, 656, 967, 1563, 1924
BceAI	ACGGC	5 cut(s)	26, 36, 380, 659, 1323
BcgI	CGANNNNNNTGC	2 cut(s)	2010, 2044
BciT130I	CCWGG	5 cut(s)	38, 215, 531, 807, 1949
BciVI	GTATCC	1 cut(s)	537
BcnI	CCSGG	5 cut(s)	206, 290, 368, 931, 932
BcoDI	GTCTC	4 cut(s)	420, 931, 1143, 1239
BfaI	CTAG	1 cut(s)	989
BfmI	CTRYAG	1 cut(s)	1327
BfuI	GTATCC	1 cut(s)	537
BlpI	GCTNAGC	2 cut(s)	114, 705
BmcAI	AGTACT	1 cut(s)	377
Bme18I	GGWCC	1 cut(s)	1097
BmeT110I	CYCGRG	3 cut(s)	419, 930, 1809
BmgT120I	GGNCC	2 cut(s)	645, 1097
BmiI	GGNNCC	4 cut(s)	478, 646, 1800, 1873
BmsI	GCATC	3 cut(s)	63, 1603, 2023
BpiI	GAAGAC	1 cut(s)	1587
BplI	GAGNNNNNCTC	2 cut(s)	877, 909
Bpu10I	CCTNAGC	1 cut(s)	1911
Bpu1102I	GCTNAGC	2 cut(s)	114, 705
BpuEI	CTTGAG	3 cut(s)	143, 1028, 1268
BpuMI	CCSGG	5 cut(s)	206, 290, 368, 931, 932
BsaHI	GRCGYC	2 cut(s)	260, 1260
BsaJI	CCNNGG	5 cut(s)	36, 144, 588, 930, 1348
BsaWI	WCCGGW	2 cut(s)	348, 1795
BsaXI	ACNNNNNCTCC	4 cut(s)	447, 477, 1000, 1030
Bsc4I	CCNNNNNNNGG	4 cut(s)	641, 642, 654, 936
Bse118I	RCCGGY	1 cut(s)	262
Bse1I	ACTGG	2 cut(s)	1556, 1606
Bse3DI	GCAATG	2 cut(s)	673, 2028
BseAI	TCCGGA	1 cut(s)	1795
BseBI	CCWGG	5 cut(s)	38, 215, 531, 807, 1949
BseDI	CCNNGG	5 cut(s)	36, 144, 588, 930, 1348
BseGI	GGATG	4 cut(s)	54, 1618, 1758, 2038
BseLI	CCNNNNNNNGG	4 cut(s)	641, 642, 654, 936
BseMI	GCAATG	2 cut(s)	673, 2028
BseMII	CTCAG	6 cut(s)	128, 894, 1373, 1531, 1559, 1931
BseNI	ACTGG	2 cut(s)	1556, 1606
BseYI	CCCAGC	1 cut(s)	332
BsgI	GTGCAG	2 cut(s)	56, 458
BshFI	GGCC	7 cut(s)	41, 343, 371, 395, 647, 831, 1347
BsiHKCI	CYCGRG	3 cut(s)	419, 930, 1809
BsiSI	CCGG	8 cut(s)	205, 248, 263, 289, 349, 368, 931, 1796
BslFI	GGGAC	5 cut(s)	52, 947, 1433, 1504, 1726
BslI	CCNNNNNNNGG	4 cut(s)	641, 642, 654, 936
BsmAI	GTCTC	4 cut(s)	420, 931, 1143, 1239
BsmFI	GGGAC	5 cut(s)	52, 947, 1433, 1504, 1726
BsmI	GAATGC	2 cut(s)	1181, 1672
BsnI	GGCC	7 cut(s)	41, 343, 371, 395, 647, 831, 1347
BsoBI	CYCGRG	3 cut(s)	419, 930, 1809
Bsp1286I	GDGCHC	2 cut(s)	1284, 2014
Bsp13I	TCCGGA	1 cut(s)	1795
Bsp143I	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
Bsp1720I	GCTNAGC	2 cut(s)	114, 705
Bsp19I	CCATGG	1 cut(s)	1348
BspACI	CCGC	9 cut(s)	23, 49, 52, 61, 149, 1369, 1448, 1488, 1736
BspANI	GGCC	7 cut(s)	41, 343, 371, 395, 647, 831, 1347
BspCNI	CTCAG	6 cut(s)	127, 893, 1374, 1530, 1558, 1932
BspEI	TCCGGA	1 cut(s)	1795
BspHI	TCATGA	2 cut(s)	724, 1237
BspLI	GGNNCC	4 cut(s)	478, 646, 1800, 1873
BspMAI	CTGCAG	1 cut(s)	1331
BspPI	GGATC	7 cut(s)	8, 344, 571, 596, 1589, 1793, 1806
BsrDI	GCAATG	2 cut(s)	673, 2028
BsrFI	RCCGGY	1 cut(s)	262
BsrI	ACTGG	2 cut(s)	1556, 1606
BssAI	RCCGGY	1 cut(s)	262
BssECI	CCNNGG	5 cut(s)	36, 144, 588, 930, 1348
BssMI	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
BssNI	GRCGYC	2 cut(s)	260, 1260
BssSI	CACGAG	2 cut(s)	169, 894
BssT1I	CCWWGG	2 cut(s)	144, 1348
Bst2BI	CACGAG	2 cut(s)	169, 894
Bst2UI	CCWGG	5 cut(s)	38, 215, 531, 807, 1949
Bst4CI	ACNGT	5 cut(s)	163, 446, 697, 1429, 1501
Bst6I	CTCTTC	1 cut(s)	807
BstACI	GRCGYC	2 cut(s)	260, 1260
BstC8I	GCNNGC	4 cut(s)	200, 1088, 1167, 1670
BstDSI	CCRYGG	1 cut(s)	1348
BstF5I	GGATG	4 cut(s)	54, 1618, 1758, 2038
BstHHI	GCGC	4 cut(s)	140, 1086, 1668, 1854
BstKTI	GATC	9 cut(s)	16, 339, 566, 604, 1050, 1456, 1597, 1801, 1837
BstMAI	GTCTC	4 cut(s)	420, 931, 1143, 1239
BstMBI	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
BstMWI	GCNNNNNNNGC	9 cut(s)	124, 146, 250, 259, 715, 1061, 1307, 1377, 2018
BstNI	CCWGG	5 cut(s)	38, 215, 531, 807, 1949
BstNSI	RCATGY	1 cut(s)	415
BstSFI	CTRYAG	1 cut(s)	1327
BstV2I	GAAGAC	1 cut(s)	1587
BstX2I	RGATCY	3 cut(s)	13, 1594, 1798
BstXI	CCANNNNNNTGG	2 cut(s)	839, 1094
BstYI	RGATCY	3 cut(s)	13, 1594, 1798
BsuI	GTATCC	1 cut(s)	537
BsuRI	GGCC	7 cut(s)	41, 343, 371, 395, 647, 831, 1347
BtgI	CCRYGG	1 cut(s)	1348
BtsCI	GGATG	4 cut(s)	54, 1618, 1758, 2038
BtsIMutI	CAGTG	4 cut(s)	888, 1434, 1497, 1563
Cac8I	GCNNGC	4 cut(s)	200, 1088, 1167, 1670
CaiI	CAGNNNCTG	1 cut(s)	1562
CciI	TCATGA	2 cut(s)	724, 1237
CfoI	GCGC	4 cut(s)	140, 1086, 1668, 1854
Cfr10I	RCCGGY	1 cut(s)	262
Cfr13I	GGNCC	2 cut(s)	645, 1097
Cfr9I	CCCGGG	1 cut(s)	930
CseI	GACGC	2 cut(s)	268, 394
Csp6I	GTAC	6 cut(s)	88, 352, 376, 442, 464, 1936
CviQI	GTAC	6 cut(s)	88, 352, 376, 442, 464, 1936
DpnI	GATC	9 cut(s)	15, 338, 565, 603, 1049, 1455, 1596, 1800, 1836
DpnII	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
EaeI	YGGCCR	2 cut(s)	369, 829
Eam1104I	CTCTTC	1 cut(s)	807
EarI	CTCTTC	1 cut(s)	807
EciI	GGCGGA	2 cut(s)	38, 1751
Eco130I	CCWWGG	2 cut(s)	144, 1348
Eco147I	AGGCCT	1 cut(s)	343
Eco24I	GRGCYC	2 cut(s)	1284, 2014
Eco47I	GGWCC	1 cut(s)	1097
Eco57I	CTGAAG	3 cut(s)	239, 1014, 1134
Eco88I	CYCGRG	3 cut(s)	419, 930, 1809
EcoRII	CCWGG	5 cut(s)	36, 213, 529, 805, 1947
EcoT14I	CCWWGG	2 cut(s)	144, 1348
EcoT22I	ATGCAT	2 cut(s)	1183, 1846
EcoT38I	GRGCYC	2 cut(s)	1284, 2014
ErhI	CCWWGG	2 cut(s)	144, 1348
FaqI	GGGAC	5 cut(s)	52, 947, 1433, 1504, 1726
FauI	CCCGC	2 cut(s)	1441, 1481
FblI	GTMKAC	4 cut(s)	408, 580, 855, 1977
FokI	GGATG	4 cut(s)	41, 1625, 1745, 2045
FriOI	GRGCYC	2 cut(s)	1284, 2014
FspBI	CTAG	1 cut(s)	989
FspI	TGCGCA	1 cut(s)	1667
GlaI	GCGC	4 cut(s)	139, 1085, 1667, 1853
GsaI	CCCAGC	1 cut(s)	336
HaeIII	GGCC	7 cut(s)	41, 343, 371, 395, 647, 831, 1347
HapII	CCGG	8 cut(s)	205, 248, 263, 289, 349, 368, 931, 1796
HgaI	GACGC	2 cut(s)	268, 394
HhaI	GCGC	4 cut(s)	140, 1086, 1668, 1854
Hin1I	GRCGYC	2 cut(s)	260, 1260
Hin6I	GCGC	4 cut(s)	138, 1084, 1666, 1852
HinP1I	GCGC	4 cut(s)	138, 1084, 1666, 1852
HincII	GTYRAC	3 cut(s)	166, 409, 1366
HindII	GTYRAC	3 cut(s)	166, 409, 1366
HpaII	CCGG	8 cut(s)	205, 248, 263, 289, 349, 368, 931, 1796
HphI	GGTGA	6 cut(s)	334, 1004, 1268, 1463, 1712, 1963
Hpy166II	GTNNAC	8 cut(s)	166, 409, 581, 856, 956, 995, 1366, 1978
Hpy8I	GTNNAC	8 cut(s)	166, 409, 581, 856, 956, 995, 1366, 1978
Hpy99I	CGWCG	4 cut(s)	359, 362, 410, 2063
HpyAV	CCTTC	3 cut(s)	271, 772, 1409
HpyCH4III	ACNGT	5 cut(s)	163, 446, 697, 1429, 1501
HpyCH4IV	ACGT	5 cut(s)	354, 1196, 1260, 1409, 2058
HpyF10VI	GCNNNNNNNGC	9 cut(s)	124, 146, 250, 259, 715, 1061, 1307, 1377, 2018
HpySE526I	ACGT	5 cut(s)	354, 1196, 1260, 1409, 2058
Hsp92I	GRCGYC	2 cut(s)	260, 1260
HspAI	GCGC	4 cut(s)	138, 1084, 1666, 1852
Kpn2I	TCCGGA	1 cut(s)	1795
Kzo9I	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
LmnI	GCTCC	4 cut(s)	250, 292, 863, 1849
LweI	GCATC	3 cut(s)	63, 1603, 2023
MaeI	CTAG	1 cut(s)	989
MaeII	ACGT	5 cut(s)	354, 1196, 1260, 1409, 2058
MaeIII	GTNAC	5 cut(s)	1256, 1294, 1501, 1558, 2002
MalI	GATC	9 cut(s)	15, 338, 565, 603, 1049, 1455, 1596, 1800, 1836
MboI	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
MboII	GAAGA	4 cut(s)	794, 896, 1020, 1592
MfeI	CAATTG	3 cut(s)	1059, 1247, 2016
MflI	RGATCY	3 cut(s)	13, 1594, 1798
MhlI	GDGCHC	2 cut(s)	1284, 2014
MlsI	TGGCCA	1 cut(s)	831
MluCI	AATT	6 cut(s)	810, 1059, 1247, 1478, 1917, 2016
MluNI	TGGCCA	1 cut(s)	831
MlyI	GAGTC	5 cut(s)	715, 1532, 1723, 1748, 1760
MmeI	TCCRAC	5 cut(s)	41, 207, 1590, 2010, 2041
Mox20I	TGGCCA	1 cut(s)	831
Mph1103I	ATGCAT	2 cut(s)	1183, 1846
MroI	TCCGGA	1 cut(s)	1795
MscI	TGGCCA	1 cut(s)	831
MseI	TTAA	8 cut(s)	426, 468, 869, 909, 1154, 1319, 1715, 2068
MslI	CAYNNNNRTG	2 cut(s)	825, 1353
Msp20I	TGGCCA	1 cut(s)	831
MspA1I	CMGCKG	1 cut(s)	61
MspI	CCGG	8 cut(s)	205, 248, 263, 289, 349, 368, 931, 1796
MunI	CAATTG	3 cut(s)	1059, 1247, 2016
Mva1269I	GAATGC	2 cut(s)	1181, 1672
MvaI	CCWGG	5 cut(s)	38, 215, 531, 807, 1949
MwoI	GCNNNNNNNGC	9 cut(s)	124, 146, 250, 259, 715, 1061, 1307, 1377, 2018
NciI	CCSGG	5 cut(s)	206, 290, 368, 931, 932
NcoI	CCATGG	1 cut(s)	1348
NdeII	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
NlaIV	GGNNCC	4 cut(s)	478, 646, 1800, 1873
NmuCI	GTSAC	5 cut(s)	1256, 1294, 1501, 1558, 2002
NsbI	TGCGCA	1 cut(s)	1667
NsiI	ATGCAT	2 cut(s)	1183, 1846
NspI	RCATGY	1 cut(s)	415
PaeR7I	CTCGAG	1 cut(s)	1809
PagI	TCATGA	2 cut(s)	724, 1237
PceI	AGGCCT	1 cut(s)	343
PciI	ACATGT	1 cut(s)	411
PcsI	WCGNNNNNNNCGW	1 cut(s)	167
PctI	GAATGC	2 cut(s)	1181, 1672
PfeI	GAWTC	5 cut(s)	32, 873, 888, 1334, 1705
PflMI	CCANNNNNTGG	1 cut(s)	654
PfoI	TCCNGGA	2 cut(s)	213, 805
PleI	GAGTC	5 cut(s)	715, 1531, 1723, 1747, 1759
PpsI	GAGTC	5 cut(s)	715, 1531, 1723, 1747, 1759
PscI	ACATGT	1 cut(s)	411
PsiI	TTATAA	1 cut(s)	623
Psp6I	CCWGG	5 cut(s)	36, 213, 529, 805, 1947
PspFI	CCCAGC	1 cut(s)	332
PspGI	CCWGG	5 cut(s)	36, 213, 529, 805, 1947
PspN4I	GGNNCC	4 cut(s)	478, 646, 1800, 1873
PspPI	GGNCC	2 cut(s)	645, 1097
PstI	CTGCAG	1 cut(s)	1331
PstNI	CAGNNNCTG	1 cut(s)	1562
PsuI	RGATCY	3 cut(s)	13, 1594, 1798
RsaI	GTAC	6 cut(s)	89, 353, 377, 443, 465, 1937
RsaNI	GTAC	6 cut(s)	88, 352, 376, 442, 464, 1936
RseI	CAYNNNNRTG	2 cut(s)	825, 1353
SalI	GTCGAC	1 cut(s)	407
SaqAI	TTAA	8 cut(s)	426, 468, 869, 909, 1154, 1319, 1715, 2068
Sau3AI	GATC	9 cut(s)	13, 336, 563, 601, 1047, 1453, 1594, 1798, 1834
Sau96I	GGNCC	2 cut(s)	645, 1097
ScaI	AGTACT	1 cut(s)	377
SchI	GAGTC	5 cut(s)	715, 1532, 1723, 1748, 1760
SduI	GDGCHC	2 cut(s)	1284, 2014
SfaNI	GCATC	3 cut(s)	63, 1603, 2023
SfcI	CTRYAG	1 cut(s)	1327
Sfr274I	CTCGAG	1 cut(s)	1809
SgrAI	CRCCGGYG	1 cut(s)	262
SinI	GGWCC	1 cut(s)	1097
SlaI	CTCGAG	1 cut(s)	1809
SmaI	CCCGGG	1 cut(s)	932
SmiMI	CAYNNNNRTG	2 cut(s)	825, 1353
SmlI	CTYRAG	4 cut(s)	122, 1043, 1283, 1809
SmoI	CTYRAG	4 cut(s)	122, 1043, 1283, 1809
Sse9I	AATT	6 cut(s)	810, 1059, 1247, 1478, 1917, 2016
SseBI	AGGCCT	1 cut(s)	343
SsiI	CCGC	9 cut(s)	23, 49, 52, 61, 149, 1369, 1448, 1488, 1736
SspI	AATATT	1 cut(s)	1217
SspMI	CTAG	1 cut(s)	989
StuI	AGGCCT	1 cut(s)	343
StyI	CCWWGG	2 cut(s)	144, 1348
TaaI	ACNGT	5 cut(s)	163, 446, 697, 1429, 1501
TaiI	ACGT	5 cut(s)	357, 1199, 1263, 1412, 2061
TaqI	TCGA	7 cut(s)	360, 408, 513, 627, 1567, 1636, 1810
TaqII	GACCGA	1 cut(s)	1004
TasI	AATT	6 cut(s)	810, 1059, 1247, 1478, 1917, 2016
TatI	WGTACW	3 cut(s)	375, 441, 463
TauI	GCSGC	3 cut(s)	51, 54, 152
TfiI	GAWTC	5 cut(s)	32, 873, 888, 1334, 1705
Tru1I	TTAA	8 cut(s)	426, 468, 869, 909, 1154, 1319, 1715, 2068
Tru9I	TTAA	8 cut(s)	426, 468, 869, 909, 1154, 1319, 1715, 2068
TscAI	CASTG	4 cut(s)	888, 1434, 1504, 1563
TseFI	GTSAC	5 cut(s)	1256, 1294, 1501, 1558, 2002
Tsp45I	GTSAC	5 cut(s)	1256, 1294, 1501, 1558, 2002
TspDTI	ATGAA	6 cut(s)	317, 668, 754, 1241, 1257, 1544
TspMI	CCCGGG	1 cut(s)	930
TspRI	CASTG	4 cut(s)	888, 1434, 1504, 1563
Van91I	CCANNNNNTGG	1 cut(s)	654
VpaK11BI	GGWCC	1 cut(s)	1097
XapI	RAATTY	1 cut(s)	1478
XceI	RCATGY	1 cut(s)	415
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1201
XhoI	CTCGAG	1 cut(s)	1809
XmaI	CCCGGG	1 cut(s)	930
XmiI	GTMKAC	4 cut(s)	408, 580, 855, 1977
XspI	CTAG	1 cut(s)	989
ZraI	GACGTC	1 cut(s)	1261
ZrmI	AGTACT	1 cut(s)	377
Zsp2I	ATGCAT	2 cut(s)	1183, 1846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.