Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0012504

Physical Location & Seq

Genomic Coordinates Forward (+)

26291209 .. 26298097

Gene Structure 6889 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G019640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGCAGGCCCCGAACCGAGATCCAACGGCGGAGGACGACTCTCAGTCCGCCGCGCGGCGCATCCAACGGCTGTCCCTCCACCTGACGCCGGCCGCGATTTCACCGGACGACCACAGCCAAATCTCGAGGCTGGAGTGCGCCAAGGCGGCGGCAAAGCTCAACGTGGACACGAAGAGTCTGTCGAGCTACATGAGAGGAAAGCACAGGGAAATCCAAGATAAGGTTATGGACTACTTCAACAACAGGCCTGACCTTCAGACTCCGGTGGAGATTTTGAAGGACGATCACCGCGAGCTTTGTATGAGGCAGCTTACTGGTTTGGTTAGGGAGGCCGGAATCAGACCGTTCCGGTACGTCGTTGAAGATCCGGCCAAGTACTTTGCGGTTATGGAAGCCGTGGGCAGCGTCGACATGTCGCTTGGGATCAAGATGGGGGTGCAGTACAGTCTTTGGGGAGGTTCTGTAATCAACTTAGGAACTAAGAAACACAAGGATAAGTACTTCGACGGTATTGACAATCTGGAGTATCCAGGTTGTTTTGCTATGACTGAACTGCATCATGGCTCAAATGTTCAAGGTCTACAAACAATGGCAACATTTGATCCGATCACAGATGAGTTTGTAATTGACACACCAAATGATGGGGCCATCAAATGGTGGATTGGCAATGCTGCTGTTCATGGCAAGTTTGCCACTGTTTTTGCTAAGCTGATGTTGCCGACACATGATAAAAGAGGAGTATCTGATATGGGTGTCCATGCCTTCATTGTACCAATAAGGGATATGAAGACTCATCAAACTCTTCCAGGAATTGAGATAAACGATTGTGGCCATAAGGTTGGTCTGAATGGGGTGGACAATGGAGCATTAAGATTCCGCGCAGTGAGAATCCCTCGTGATAATCTTCTTAATCGCTTTGGAGATGTCTCTCGGGATGGTAAATACACAAGTAGTCTACCATCTATAAATAAAAGATTTGCTGCCACACTAGGAGAACTTGTAGGTGGGAGGGTCGGCCTTGCATATTCTTCAGGCAGTGTCTTGAAGGTTGCTGGCACAATTGCAATCCGATATTCTCTACTGCGTCAGCAGTTTGGTCCTCCAAAGCAACCTGAAGTCAGTATTCTTGATTACCAATCCCAGCAGCACAAGTTAATGCCAATGCTGGCTTCAACTTACGCATTCCATTTTGCCACACTGCATTTGGTGGAGAAATATTCAGAGATGAAGAAGACTCATGATGAACAATTGGTTGCAGATGTCCATTCTCTTTCAGCAGGGCTCAAGGCTTATGTGACGTCATATACAGCAAAGTCATTGAGTATCTGTAGGGAAGCTTGTGGAGGCCATGGGTACGCTGCTGTCAACCGCTTTGGCAGTTTGAGGAATGACCATGACATTTTTCAGACATTCGAAGGGGACAACACGGTGCTTCTACAGCAGGTAGCAGGTGATCTGTTGAAGCAATACAAGGAGAAGTTTCAAGGCGGGACACTCACAGTTACCTGGAATTACCTTAGAGAATCCATGAACTCATACCTATCTCAGCCAAATCCGGTGACTGCTCGATGGGAAAGTGAAGACCATTTGCGAGATCCAAAGTTCCAGTTGGATGCTTTCAGATACCGAACTTCTCGACTACTTCAAAGTGTTGCTGTTAGACTTCGCAAGCACTCTAAAACTCTTGGAAGTTTTGGCGCTTGGAATAGATGCTTAAACCACCTTTTGACACTTGCAGAGTCCCATATTGAATCTGTCATCCTTGCCAAGTTTATAGAAGCTGTGCAGAACTGTCCAGATGCAAGTTCTCGAGCAGCTCTGAAACTCGTCTGTGATCTTTATGCCTTGGATCAAATATGGAAAGACATAGGAACCTATCGTAATGTTGATTATGTTGCACCAAACAAAGCTAAGGCAATCCACAAGCTCACTGCATACCTGAGTTTCCGAGTGAGAAATATAGCAGGGGAACTCATAGACGCATTTGATATTCCAGATTATGTTACGCGTGCCCCAATTGCAATGCAGTCAGATGCCTACTCTCAGTACACACAGCATGTGGGATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042579 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071704 GO:0072329 GO:0097159 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_1 Acyl-CoA_dh_M

Protein Analysis

689

Amino Acids

77.13

Weight (kDa)

8.77

Isoelectric Point (pI)

38.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_M	PF02770	179 - 295	7.2e-12	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	328 - 484	3.2e-09	Acyl-CoA dehydrogenase, C-terminal domain
ACOX_C_alpha1	PF22924	331 - 486	2.8e-50	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	532 - 685	2.3e-24	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65110 AT5G65110
fragaria_vesca	FvH4_1g21250
malus_domestica	MD01G1026400.v1.1 MD15G1327100.v1.1
prunus_persica	Prupe.6G181800_v2.0.a1
pyrus_communis	pycom01g05460 pycom395g00040
rosa_chinensis	RchiOBHm_Chr2g0115091
rosa_laevigata	RLG00000018156
rosa_multiflora	Rmu_sc0002784.1_g000002
rosa_roxburghii	Rroxscaffold_2G00130560
rosa_rugosa	Rorug02G0193300 Rorug02G0193400
rosa_samantha	Rh2AG251800 Rh2BG261400 Rh2CG256300 Rh2DG259900
rosa_wichuraiana	Rw2G019640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1439
AatII	GACGTC	1 cut(s)	1301
Acc36I	ACCTGC	2 cut(s)	1432, 1439
AccB7I	CCANNNNNTGG	2 cut(s)	641, 654
AccI	GTMKAC	3 cut(s)	408, 580, 955
AccII	CGCG	6 cut(s)	53, 55, 95, 291, 879, 2008
AclWI	GGATC	6 cut(s)	14, 359, 431, 596, 1589, 1857
AcoI	YGGCCR	3 cut(s)	90, 369, 829
AcuI	CTGAAG	3 cut(s)	239, 1014, 1134
AcyI	GRCGYC	2 cut(s)	86, 1298
AfaI	GTAC	7 cut(s)	353, 377, 443, 500, 771, 1355, 2048
AfiI	CCNNNNNNNGG	5 cut(s)	54, 88, 220, 641, 654
AflIII	ACRYGT	2 cut(s)	411, 2006
AhdI	GACNNNNNGTC	1 cut(s)	43
AjnI	CCWGG	3 cut(s)	529, 805, 1505
AjuI	GAANNNNNNNTTGG	2 cut(s)	1153, 1185
Alw26I	GTCTC	1 cut(s)	931
AlwI	GGATC	6 cut(s)	14, 359, 431, 596, 1589, 1857
Ama87I	CYCGRG	3 cut(s)	124, 930, 1809
AoxI	GGCC	9 cut(s)	6, 90, 245, 330, 369, 645, 829, 1015, 1345
ApeKI	GCWGC	7 cut(s)	307, 402, 671, 980, 1144, 1358, 1814
ArsI	GACNNNNNNTTYG	2 cut(s)	164, 196
AspLEI	GCGC	5 cut(s)	55, 60, 140, 881, 1700
AspS9I	GGNCC	3 cut(s)	7, 645, 1097
AsuHPI	GGTGA	4 cut(s)	93, 278, 1463, 1570
AsuII	TTCGAA	1 cut(s)	1413
AvaI	CYCGRG	3 cut(s)	124, 930, 1809
AvaII	GGWCC	1 cut(s)	1097
BaeGI	GKGCMC	1 cut(s)	2014
BalI	TGGCCA	1 cut(s)	831
BanII	GRGCYC	1 cut(s)	1284
BauI	CACGAG	1 cut(s)	894
BbsI	GAAGAC	3 cut(s)	794, 1238, 1587
BbvI	GCAGC	7 cut(s)	319, 414, 658, 967, 1156, 1345, 1826
BccI	CCATC	6 cut(s)	424, 635, 656, 929, 967, 1563
BceAI	ACGGC	3 cut(s)	42, 83, 380
BciT130I	CCWGG	3 cut(s)	531, 807, 1507
BciVI	GTATCC	1 cut(s)	537
BcoDI	GTCTC	1 cut(s)	931
BfaI	CTAG	1 cut(s)	989
BfmI	CTRYAG	2 cut(s)	1327, 1436
BfoI	RGCGCY	1 cut(s)	1701
BfuAI	ACCTGC	2 cut(s)	1432, 1439
BfuI	GTATCC	1 cut(s)	537
BglI	GCCNNNNNGGC	1 cut(s)	146
BlpI	GCTNAGC	1 cut(s)	705
BmcAI	AGTACT	2 cut(s)	377, 500
Bme1390I	CCNGG	3 cut(s)	531, 807, 1507
Bme18I	GGWCC	1 cut(s)	1097
BmeRI	GACNNNNNGTC	1 cut(s)	43
BmeT110I	CYCGRG	3 cut(s)	124, 930, 1809
BmgT120I	GGNCC	3 cut(s)	7, 645, 1097
BmiI	GGNNCC	3 cut(s)	9, 646, 1873
BmrFI	CCNGG	3 cut(s)	531, 807, 1507
BmsI	GCATC	6 cut(s)	69, 565, 1603, 1700, 1789, 2023
BpiI	GAAGAC	3 cut(s)	794, 1238, 1587
BplI	GAGNNNNNCTC	4 cut(s)	23, 55, 877, 909
BpmI	CTGGAG	2 cut(s)	152, 542
Bpu10I	CCTNAGC	1 cut(s)	1911
Bpu1102I	GCTNAGC	1 cut(s)	705
Bpu14I	TTCGAA	1 cut(s)	1413
BpuEI	CTTGAG	1 cut(s)	1268
BsaHI	GRCGYC	2 cut(s)	86, 1298
BsaJI	CCNNGG	4 cut(s)	141, 396, 1348, 1845
BsaWI	WCCGGW	4 cut(s)	103, 262, 348, 1555
BsaXI	ACNNNNNCTCC	4 cut(s)	447, 477, 984, 1014
Bsc4I	CCNNNNNNNGG	5 cut(s)	54, 88, 220, 641, 654
Bse118I	RCCGGY	1 cut(s)	88
Bse1I	ACTGG	2 cut(s)	319, 1606
Bse3DI	GCAATG	2 cut(s)	673, 2028
BseBI	CCWGG	3 cut(s)	531, 807, 1507
BseDI	CCNNGG	4 cut(s)	141, 396, 1348, 1845
BseGI	GGATG	4 cut(s)	60, 940, 1618, 1758
BseLI	CCNNNNNNNGG	5 cut(s)	54, 88, 220, 641, 654
BseMI	GCAATG	2 cut(s)	673, 2028
BseMII	CTCAG	4 cut(s)	56, 1559, 1931, 2057
BseNI	ACTGG	2 cut(s)	319, 1606
BseRI	GAGGAG	1 cut(s)	750
BseSI	GKGCMC	1 cut(s)	2014
BseX3I	CGGCCG	1 cut(s)	90
BseXI	GCAGC	7 cut(s)	319, 414, 658, 967, 1156, 1345, 1826
BseYI	CCCAGC	1 cut(s)	1140
BsgI	GTGCAG	2 cut(s)	458, 1805
Bsh1236I	CGCG	6 cut(s)	53, 55, 95, 291, 879, 2008
Bsh1285I	CGRYCG	1 cut(s)	93
BshFI	GGCC	9 cut(s)	8, 92, 247, 332, 371, 647, 831, 1017, 1347
BsiEI	CGRYCG	1 cut(s)	93
BsiHKCI	CYCGRG	3 cut(s)	124, 930, 1809
BsiSI	CCGG	7 cut(s)	89, 104, 263, 333, 349, 368, 1556
BslFI	GGGAC	4 cut(s)	58, 1433, 1504, 1726
BslI	CCNNNNNNNGG	5 cut(s)	54, 88, 220, 641, 654
BsmAI	GTCTC	1 cut(s)	931
BsmFI	GGGAC	4 cut(s)	58, 1433, 1504, 1726
BsmI	GAATGC	1 cut(s)	1181
BsnI	GGCC	9 cut(s)	8, 92, 247, 332, 371, 647, 831, 1017, 1347
BsoBI	CYCGRG	3 cut(s)	124, 930, 1809
Bsp119I	TTCGAA	1 cut(s)	1413
Bsp1286I	GDGCHC	2 cut(s)	1284, 2014
Bsp1720I	GCTNAGC	1 cut(s)	705
Bsp19I	CCATGG	1 cut(s)	1348
BspANI	GGCC	9 cut(s)	8, 92, 247, 332, 371, 647, 831, 1017, 1347
BspCNI	CTCAG	4 cut(s)	55, 1558, 1932, 2056
BspFNI	CGCG	6 cut(s)	53, 55, 95, 291, 879, 2008
BspHI	TCATGA	1 cut(s)	1237
BspLI	GGNNCC	3 cut(s)	9, 646, 1873
BspMI	ACCTGC	2 cut(s)	1432, 1439
BspPI	GGATC	6 cut(s)	14, 359, 431, 596, 1589, 1857
BspT104I	TTCGAA	1 cut(s)	1413
BsrDI	GCAATG	2 cut(s)	673, 2028
BsrFI	RCCGGY	1 cut(s)	88
BsrI	ACTGG	2 cut(s)	319, 1606
BssAI	RCCGGY	1 cut(s)	88
BssECI	CCNNGG	4 cut(s)	141, 396, 1348, 1845
BssNI	GRCGYC	2 cut(s)	86, 1298
BssSI	CACGAG	1 cut(s)	894
BssT1I	CCWWGG	3 cut(s)	141, 1348, 1845
Bst2BI	CACGAG	1 cut(s)	894
Bst2UI	CCWGG	3 cut(s)	531, 807, 1507
Bst4CI	ACNGT	7 cut(s)	345, 446, 509, 697, 1429, 1501, 1793
Bst6I	CTCTTC	2 cut(s)	167, 807
BstACI	GRCGYC	2 cut(s)	86, 1298
BstBI	TTCGAA	1 cut(s)	1413
BstC8I	GCNNGC	7 cut(s)	6, 90, 293, 1054, 1167, 1670, 2010
BstDEI	CTNAG	9 cut(s)	42, 472, 480, 705, 1517, 1545, 1911, 1940, 2043
BstDSI	CCRYGG	2 cut(s)	396, 1348
BstF5I	GGATG	4 cut(s)	60, 940, 1618, 1758
BstFNI	CGCG	6 cut(s)	53, 55, 95, 291, 879, 2008
BstH2I	RGCGCY	1 cut(s)	1701
BstHHI	GCGC	5 cut(s)	55, 60, 140, 881, 1700
BstMAI	GTCTC	1 cut(s)	931
BstMCI	CGRYCG	1 cut(s)	93
BstMWI	GCNNNNNNNGC	4 cut(s)	146, 715, 1438, 2018
BstNI	CCWGG	3 cut(s)	531, 807, 1507
BstNSI	RCATGY	2 cut(s)	415, 2060
BstSCI	CCNGG	3 cut(s)	529, 805, 1505
BstSFI	CTRYAG	2 cut(s)	1327, 1436
BstSLI	GKGCMC	1 cut(s)	2014
BstUI	CGCG	6 cut(s)	53, 55, 95, 291, 879, 2008
BstV1I	GCAGC	7 cut(s)	319, 414, 658, 967, 1156, 1345, 1826
BstV2I	GAAGAC	3 cut(s)	794, 1238, 1587
BstX2I	RGATCY	3 cut(s)	19, 364, 1594
BstXI	CCANNNNNNTGG	1 cut(s)	839
BstYI	RGATCY	3 cut(s)	19, 364, 1594
BstZI	CGGCCG	1 cut(s)	90
BsuI	GTATCC	1 cut(s)	537
BsuRI	GGCC	9 cut(s)	8, 92, 247, 332, 371, 647, 831, 1017, 1347
BtgI	CCRYGG	2 cut(s)	396, 1348
BtsCI	GGATG	4 cut(s)	60, 940, 1618, 1758
BtsI	GCAGTG	4 cut(s)	888, 1042, 1196, 1929
BtsIMutI	CAGTG	5 cut(s)	693, 888, 1042, 1196, 1929
BveI	ACCTGC	2 cut(s)	1432, 1439
Cac8I	GCNNGC	7 cut(s)	6, 90, 293, 1054, 1167, 1670, 2010
CciI	TCATGA	1 cut(s)	1237
CfoI	GCGC	5 cut(s)	55, 60, 140, 881, 1700
Cfr10I	RCCGGY	1 cut(s)	88
Cfr13I	GGNCC	3 cut(s)	7, 645, 1097
CseI	GACGC	4 cut(s)	94, 394, 1073, 1988
Csp6I	GTAC	7 cut(s)	352, 376, 442, 499, 770, 1354, 2047
CspCI	CAANNNNNGTGG	2 cut(s)	682, 717
CviQI	GTAC	7 cut(s)	352, 376, 442, 499, 770, 1354, 2047
DdeI	CTNAG	9 cut(s)	42, 472, 480, 705, 1517, 1545, 1911, 1940, 2043
DriI	GACNNNNNGTC	1 cut(s)	43
EaeI	YGGCCR	3 cut(s)	90, 369, 829
EagI	CGGCCG	1 cut(s)	90
Eam1104I	CTCTTC	2 cut(s)	167, 807
Eam1105I	GACNNNNNGTC	1 cut(s)	43
EarI	CTCTTC	2 cut(s)	167, 807
EciI	GGCGGA	2 cut(s)	37, 44
EclXI	CGGCCG	1 cut(s)	90
Eco130I	CCWWGG	3 cut(s)	141, 1348, 1845
Eco147I	AGGCCT	1 cut(s)	247
Eco24I	GRGCYC	1 cut(s)	1284
Eco47I	GGWCC	1 cut(s)	1097
Eco52I	CGGCCG	1 cut(s)	90
Eco57I	CTGAAG	3 cut(s)	239, 1014, 1134
Eco88I	CYCGRG	3 cut(s)	124, 930, 1809
EcoO109I	RGGNCCY	1 cut(s)	7
EcoRII	CCWGG	3 cut(s)	529, 805, 1505
EcoT14I	CCWWGG	3 cut(s)	141, 1348, 1845
EcoT38I	GRGCYC	1 cut(s)	1284
ErhI	CCWWGG	3 cut(s)	141, 1348, 1845
FaqI	GGGAC	4 cut(s)	58, 1433, 1504, 1726
FauI	CCCGC	1 cut(s)	1481
FblI	GTMKAC	3 cut(s)	408, 580, 955
FokI	GGATG	4 cut(s)	47, 947, 1625, 1745
FriOI	GRGCYC	1 cut(s)	1284
FspBI	CTAG	1 cut(s)	989
GlaI	GCGC	5 cut(s)	54, 59, 139, 880, 1699
GsaI	CCCAGC	1 cut(s)	1144
GsuI	CTGGAG	2 cut(s)	152, 542
HaeII	RGCGCY	1 cut(s)	1701
HaeIII	GGCC	9 cut(s)	8, 92, 247, 332, 371, 647, 831, 1017, 1347
HapII	CCGG	7 cut(s)	89, 104, 263, 333, 349, 368, 1556
HgaI	GACGC	4 cut(s)	94, 394, 1073, 1988
HhaI	GCGC	5 cut(s)	55, 60, 140, 881, 1700
Hin1I	GRCGYC	2 cut(s)	86, 1298
Hin6I	GCGC	5 cut(s)	53, 58, 138, 879, 1698
HinP1I	GCGC	5 cut(s)	53, 58, 138, 879, 1698
HincII	GTYRAC	2 cut(s)	409, 1366
HindII	GTYRAC	2 cut(s)	409, 1366
HindIII	AAGCTT	1 cut(s)	1335
HpaII	CCGG	7 cut(s)	89, 104, 263, 333, 349, 368, 1556
HphI	GGTGA	4 cut(s)	93, 278, 1463, 1570
Hpy166II	GTNNAC	7 cut(s)	166, 409, 581, 856, 956, 1366, 2049
Hpy8I	GTNNAC	7 cut(s)	166, 409, 581, 856, 956, 1366, 2049
Hpy99I	CGWCG	3 cut(s)	359, 410, 509
HpyAV	CCTTC	5 cut(s)	263, 271, 772, 1039, 1409
HpyCH4III	ACNGT	7 cut(s)	345, 446, 509, 697, 1429, 1501, 1793
HpyCH4IV	ACGT	3 cut(s)	162, 354, 1298
HpyF10VI	GCNNNNNNNGC	4 cut(s)	146, 715, 1438, 2018
HpyF3I	CTNAG	9 cut(s)	42, 472, 480, 705, 1517, 1545, 1911, 1940, 2043
HpySE526I	ACGT	3 cut(s)	162, 354, 1298
Hsp92I	GRCGYC	2 cut(s)	86, 1298
HspAI	GCGC	5 cut(s)	53, 58, 138, 879, 1698
KroI	GCCGGC	1 cut(s)	88
KroNI	GCCGGC	1 cut(s)	90
LmnI	GCTCC	1 cut(s)	863
Lsp1109I	GCAGC	7 cut(s)	319, 414, 658, 967, 1156, 1345, 1826
LweI	GCATC	6 cut(s)	69, 565, 1603, 1700, 1789, 2023
MaeI	CTAG	1 cut(s)	989
MaeII	ACGT	3 cut(s)	162, 354, 1298
MaeIII	GTNAC	4 cut(s)	1294, 1501, 1558, 2002
MboII	GAAGA	9 cut(s)	184, 374, 794, 799, 896, 1020, 1240, 1243, 1592
MfeI	CAATTG	3 cut(s)	1059, 1247, 2016
MflI	RGATCY	3 cut(s)	19, 364, 1594
MhlI	GDGCHC	2 cut(s)	1284, 2014
MlsI	TGGCCA	1 cut(s)	831
MluCI	AATT	6 cut(s)	624, 810, 1059, 1247, 1510, 2016
MluI	ACGCGT	1 cut(s)	2006
MluNI	TGGCCA	1 cut(s)	831
MlyI	GAGTC	6 cut(s)	32, 184, 253, 784, 1228, 1748
MmeI	TCCRAC	3 cut(s)	47, 88, 1590
Mox20I	TGGCCA	1 cut(s)	831
MroNI	GCCGGC	1 cut(s)	88
MscI	TGGCCA	1 cut(s)	831
MseI	TTAA	5 cut(s)	869, 909, 1154, 1715, 2068
Msp20I	TGGCCA	1 cut(s)	831
MspI	CCGG	7 cut(s)	89, 104, 263, 333, 349, 368, 1556
MspR9I	CCNGG	3 cut(s)	531, 807, 1507
MteI	GCGCNGCGC	1 cut(s)	56
MunI	CAATTG	3 cut(s)	1059, 1247, 2016
Mva1269I	GAATGC	1 cut(s)	1181
MvaI	CCWGG	3 cut(s)	531, 807, 1507
MvnI	CGCG	6 cut(s)	53, 55, 95, 291, 879, 2008
MwoI	GCNNNNNNNGC	4 cut(s)	146, 715, 1438, 2018
NaeI	GCCGGC	1 cut(s)	90
NcoI	CCATGG	1 cut(s)	1348
NgoMIV	GCCGGC	1 cut(s)	88
NlaIV	GGNNCC	3 cut(s)	9, 646, 1873
NmuCI	GTSAC	2 cut(s)	1294, 1558
NspI	RCATGY	2 cut(s)	415, 2060
NspV	TTCGAA	1 cut(s)	1413
PaeR7I	CTCGAG	2 cut(s)	124, 1809
PagI	TCATGA	1 cut(s)	1237
PaqCI	CACCTGC	1 cut(s)	1439
PceI	AGGCCT	1 cut(s)	247
PciI	ACATGT	1 cut(s)	411
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 288
PctI	GAATGC	1 cut(s)	1181
PdiI	GCCGGC	1 cut(s)	90
PfeI	GAWTC	5 cut(s)	336, 873, 888, 1523, 1751
PflMI	CCANNNNNTGG	2 cut(s)	641, 654
PfoI	TCCNGGA	1 cut(s)	805
PleI	GAGTC	6 cut(s)	32, 183, 253, 784, 1228, 1747
PpsI	GAGTC	6 cut(s)	32, 183, 253, 784, 1228, 1747
PscI	ACATGT	1 cut(s)	411
Psp6I	CCWGG	3 cut(s)	529, 805, 1505
PspFI	CCCAGC	1 cut(s)	1140
PspGI	CCWGG	3 cut(s)	529, 805, 1505
PspN4I	GGNNCC	3 cut(s)	9, 646, 1873
PspPI	GGNCC	3 cut(s)	7, 645, 1097
PsuI	RGATCY	3 cut(s)	19, 364, 1594
RsaI	GTAC	7 cut(s)	353, 377, 443, 500, 771, 1355, 2048
RsaNI	GTAC	7 cut(s)	352, 376, 442, 499, 770, 1354, 2047
SalI	GTCGAC	1 cut(s)	407
SaqAI	TTAA	5 cut(s)	869, 909, 1154, 1715, 2068
Sau96I	GGNCC	3 cut(s)	7, 645, 1097
ScaI	AGTACT	2 cut(s)	377, 500
SchI	GAGTC	6 cut(s)	32, 184, 253, 784, 1228, 1748
ScrFI	CCNGG	3 cut(s)	531, 807, 1507
SduI	GDGCHC	2 cut(s)	1284, 2014
SfaNI	GCATC	6 cut(s)	69, 565, 1603, 1700, 1789, 2023
SfcI	CTRYAG	2 cut(s)	1327, 1436
Sfr274I	CTCGAG	2 cut(s)	124, 1809
SfuI	TTCGAA	1 cut(s)	1413
SinI	GGWCC	1 cut(s)	1097
SlaI	CTCGAG	2 cut(s)	124, 1809
SmlI	CTYRAG	3 cut(s)	124, 1283, 1809
SmoI	CTYRAG	3 cut(s)	124, 1283, 1809
Sse9I	AATT	6 cut(s)	624, 810, 1059, 1247, 1510, 2016
SseBI	AGGCCT	1 cut(s)	247
SspI	AATATT	1 cut(s)	1217
SspMI	CTAG	1 cut(s)	989
StuI	AGGCCT	1 cut(s)	247
StyD4I	CCNGG	3 cut(s)	529, 805, 1505
StyI	CCWWGG	3 cut(s)	141, 1348, 1845
TaaI	ACNGT	7 cut(s)	345, 446, 509, 697, 1429, 1501, 1793
TaiI	ACGT	3 cut(s)	165, 357, 1301
TaqI	TCGA	8 cut(s)	125, 182, 408, 504, 1413, 1567, 1636, 1810
TasI	AATT	6 cut(s)	624, 810, 1059, 1247, 1510, 2016
TatI	WGTACW	4 cut(s)	375, 441, 498, 2046
TauI	GCSGC	5 cut(s)	53, 58, 95, 149, 152
TfiI	GAWTC	5 cut(s)	336, 873, 888, 1523, 1751
Tru1I	TTAA	5 cut(s)	869, 909, 1154, 1715, 2068
Tru9I	TTAA	5 cut(s)	869, 909, 1154, 1715, 2068
TscAI	CASTG	5 cut(s)	700, 888, 1042, 1203, 1936
TseFI	GTSAC	2 cut(s)	1294, 1558
TseI	GCWGC	7 cut(s)	307, 402, 671, 980, 1144, 1358, 1814
Tsp45I	GTSAC	2 cut(s)	1294, 1558
TspDTI	ATGAA	6 cut(s)	668, 754, 800, 1241, 1257, 1544
TspRI	CASTG	5 cut(s)	700, 888, 1042, 1203, 1936
Van91I	CCANNNNNTGG	2 cut(s)	641, 654
VpaK11BI	GGWCC	1 cut(s)	1097
XceI	RCATGY	2 cut(s)	415, 2060
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1201
XhoI	CTCGAG	2 cut(s)	124, 1809
XmiI	GTMKAC	3 cut(s)	408, 580, 955
XspI	CTAG	1 cut(s)	989
ZraI	GACGTC	1 cut(s)	1299
ZrmI	AGTACT	2 cut(s)	377, 500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.