Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012504

Physical Location & Seq

Genomic Coordinates Forward (+)

35353895 .. 35359436

Gene Structure 5542 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1327100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2073 bp

ATGCAGAGTCCACAACCAGATCCAACGGCGGAGGACGAATCCCAGGCCGTCAGCCGCCGCATCCAACGGCTGTCTTTACACCTGACACCTAATCACCGATATCTCCAAAACGACACCGCTCAGCAGCTTAGGCTGGTGGGGTGCGCCAAGGCGGCGAAGCTGACGGTGGACACGAGCAGCCTCTCAAACTACATGAGAGGAAAACACCGGGAAATCCAGGACAGAGTGTTGGATTACTTCAACAAGCGGCCGGAGCTTCAGACGCCGGTGGAGATTTTGAAGGACGACCATCGGGAGCTTTGTATGAAGCAGCTCGTTGGGTTGGTGAGAGAGGCCGGGATCAGGCCTTTCAGGTACGTCGTTGATGAACCGGCTAAGTACTTCGCTATTTTGGAGGCCGTTGGCAGCGTCGACATGTCTCTCGGGATTAAGATGGGGGTGCAGTACAGCCTTTGGGGAGGTTCTGTACTTAACTTAGGAACTAAAAAGCACAAGGATATGTATTTTGATGGTATCGACAATCTGGAGTATCCAGGTTGTTTTGCTATGACTGAACTGCATCATGGCTCAAATGTTCAAGGTCTTCAAACAGTGGCAACTTTTGATCCAATCACAGATGAATTTATAATCGACACACCCAATGATGGGGCAATCAAATGGTGGATTGGCAATGCTGCCGTTCATGGAAAGTTTGCTACTGTTTTTGCTAAGCTGATGTTGCCGACTCATGACACAAAAGGTTTTACTGATATGGGTGTCCATGGCTTCATTGTGCCAATAAGAGATTTCAAGACCCACCAAACTCTTCCAGGGATTGAGATACAGGATTGTGGCCACAAGGTGGGTCTGAATGGGGTAGACAATGGAGCATTAAGATTCCGGTCAGTGAGAATCCCTCGTGATAATCTTCTTAATCGATTCGGAGATGTCTCCCGGGATGGGAAATACACAAGTAGCTTACCATCTATAAATAAAAGATTTGCTGCCACTCTAGGGGAACTTGTAGGTGGGAGGGTCGGTCTTGCATATTCTTCAGTTAGTGTCCTCAAGGTCGCCGCCACAATTGCAATCCGATACTCTCTACTGCGACAGCAGTTTGGTCCTTCCAAGCAACCTGAAGTCAGTATTCTTGATTATCAGTCCCAACAACACAAGTTAATGCCAATGCTGGCTTCAACTTATGCATTGCATTTTGCCACGTCGCATTTGGTGGAGAAATATTCAGAGATGAAAAAGTCTCATGATGAACAATTGGTGGGTGACGTCCATTCCCTTTCAGCAGGGCTCAAGGCTTATGTGACGGCATATACAGCAAAGTCATTGAGTATCTGCAGGGAAGCTTGTGGAGGCCATGGGTATGCTTCTGTCAACCGCTTTGGTAGCTTGAGGAATGACCATGACATTTTTCAGACGTTTGAAGGGGACAATACAGTGCTCCTACAACAGGTTGCTGGTGATCTGTTGAAGCAATATAAGGAGAAATTTCAAGGCGGGACACTCACAGTCACATGGAGCTACCTCAGAGAGTCCATGAACTCATATCTGTCCCAGCCAAATCCAGTGACTGCTCGATGGGAAAGTGAAGACCATTTGCGAGATCCTAAGTTCCAGTTGGATGCTTTCAGATACCGAACTTCTCGATTACTTCAAAGTGTTGCAGTAAGACTGCGCAAGCATTCCAAAACTCTTGGCAGTTTTGGTGCATGGAATAGATGCTTAAATCACCTTTTGACTCTTGCAGAGTCCCACATTGAGTCTGTCATCCTTGCCAAGTTTGTTGAAGCTGTCCAGAAGTGTCCAGATCCAAGTTCTCGAGCTGCTCTGAAACTCGTCTGTGATCTTTATGCCTTGGAGCGCATTTGGAAAGACATAGGAACCTATCGTAATGTTGATTATGTCGCACCAAACAAAGCTAAGGCAATTCACAAACTGATGGAGTACCTGAGTTTTCAGGTGAGAAATATAGCAAAGGAACTTGTAGACGCATTTGACATTCCAGATTATGTTACAAGAGCCCCGATTGCAATGCAGTCAGATGCCTACTCACACTACACACACTATGTCGGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042579 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071704 GO:0072329 GO:0097159 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_1 Acyl-CoA_dh_M

Protein Analysis

691

Amino Acids

77.62

Weight (kDa)

8.83

Isoelectric Point (pI)

40.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_M	PF02770	180 - 296	1.4e-11	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	329 - 485	7.3e-10	Acyl-CoA dehydrogenase, C-terminal domain
ACOX_C_alpha1	PF22924	332 - 487	2.8e-50	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	533 - 684	1.2e-25	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65110 AT5G65110
fragaria_vesca	FvH4_1g21250
malus_domestica	MD01G1026400.v1.1 MD15G1327100.v1.1
prunus_persica	Prupe.6G181800_v2.0.a1
pyrus_communis	pycom01g05460 pycom395g00040
rosa_chinensis	RchiOBHm_Chr2g0115091
rosa_laevigata	RLG00000018156
rosa_multiflora	Rmu_sc0002784.1_g000002
rosa_roxburghii	Rroxscaffold_2G00130560
rosa_rugosa	Rorug02G0193300 Rorug02G0193400
rosa_samantha	Rh2AG251800 Rh2BG261400 Rh2CG256300 Rh2DG259900
rosa_wichuraiana	Rw2G019640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	626
AatII	GACGTC	1 cut(s)	1266
Acc16I	TGCGCA	1 cut(s)	1670
AccB7I	CCANNNNNTGG	1 cut(s)	841
AccBSI	CCGCTC	1 cut(s)	119
AccI	GTMKAC	3 cut(s)	411, 858, 1980
AciI	CCGC	9 cut(s)	29, 55, 58, 117, 152, 247, 1056, 1372, 1491
AclWI	GGATC	5 cut(s)	14, 347, 599, 1592, 1796
AcoI	YGGCCR	2 cut(s)	248, 832
AcsI	RAATTY	2 cut(s)	620, 1481
AcuI	CTGAAG	3 cut(s)	242, 1017, 1137
AcyI	GRCGYC	2 cut(s)	263, 1263
AdeI	CACNNNGTG	1 cut(s)	841
AfaI	GTAC	5 cut(s)	356, 380, 446, 468, 1940
AfiI	CCNNNNNNNGG	7 cut(s)	342, 644, 645, 841, 939, 993, 1444
AflIII	ACRYGT	1 cut(s)	414
AjiI	CACGTC	1 cut(s)	1200
AjnI	CCWGG	4 cut(s)	42, 216, 532, 808
AjuI	GAANNNNNNNTTGG	2 cut(s)	1156, 1188
Alw21I	GWGCWC	1 cut(s)	1437
Alw26I	GTCTC	3 cut(s)	423, 934, 1242
AlwI	GGATC	5 cut(s)	14, 347, 599, 1592, 1796
AlwNI	CAGNNNCTG	1 cut(s)	1565
Ama87I	CYCGRG	3 cut(s)	422, 933, 1812
AoxI	GGCC	7 cut(s)	45, 248, 333, 344, 396, 832, 1348
ApeKI	GCWGC	7 cut(s)	124, 177, 310, 405, 674, 983, 1817
ApoI	RAATTY	2 cut(s)	620, 1481
AspLEI	GCGC	3 cut(s)	146, 1671, 1857
AspS9I	GGNCC	1 cut(s)	1100
AsuC2I	CCSGG	4 cut(s)	209, 337, 934, 935
AsuHPI	GGTGA	6 cut(s)	86, 337, 1271, 1466, 1715, 1966
AvaI	CYCGRG	3 cut(s)	422, 933, 1812
AvaII	GGWCC	1 cut(s)	1100
BalI	TGGCCA	1 cut(s)	834
BanII	GRGCYC	2 cut(s)	1287, 2017
BauI	CACGAG	2 cut(s)	172, 897
BbsI	GAAGAC	2 cut(s)	575, 1590
Bbv12I	GWGCWC	1 cut(s)	1437
BbvI	GCAGC	7 cut(s)	136, 189, 322, 417, 661, 970, 1804
BccI	CCATC	8 cut(s)	297, 427, 503, 638, 932, 970, 1566, 1927
BceAI	ACGGC	6 cut(s)	32, 42, 83, 383, 662, 1317
BcgI	CGANNNNNNTGC	2 cut(s)	2008, 2042
BciT130I	CCWGG	4 cut(s)	44, 218, 534, 810
BciVI	GTATCC	1 cut(s)	540
BcnI	CCSGG	4 cut(s)	209, 337, 934, 935
BcoDI	GTCTC	3 cut(s)	423, 934, 1242
BfaI	CTAG	1 cut(s)	992
BfmI	CTRYAG	1 cut(s)	1330
BfuI	GTATCC	1 cut(s)	540
BglI	GCCNNNNNGGC	1 cut(s)	152
BlpI	GCTNAGC	2 cut(s)	120, 708
BmcAI	AGTACT	1 cut(s)	380
Bme1390I	CCNGG	8 cut(s)	44, 209, 218, 337, 534, 810, 934, 935
Bme18I	GGWCC	1 cut(s)	1100
BmeT110I	CYCGRG	3 cut(s)	422, 933, 1812
BmgBI	CACGTC	1 cut(s)	1200
BmgT120I	GGNCC	1 cut(s)	1100
BmiI	GGNNCC	1 cut(s)	1876
BmrFI	CCNGG	8 cut(s)	44, 209, 218, 337, 534, 810, 934, 935
BmsI	GCATC	5 cut(s)	69, 568, 1606, 1703, 2026
BpiI	GAAGAC	2 cut(s)	575, 1590
BplI	GAGNNNNNCTC	2 cut(s)	880, 912
BpmI	CTGGAG	1 cut(s)	545
Bpu10I	CCTNAGC	2 cut(s)	128, 1914
Bpu1102I	GCTNAGC	2 cut(s)	120, 708
BpuEI	CTTGAG	3 cut(s)	1031, 1271, 1405
BpuMI	CCSGG	4 cut(s)	209, 337, 934, 935
Bsa29I	ATCGAT	1 cut(s)	916
BsaHI	GRCGYC	2 cut(s)	263, 1263
BsaJI	CCNNGG	7 cut(s)	42, 147, 760, 809, 933, 1351, 1848
BsaWI	WCCGGW	1 cut(s)	879
BsaXI	ACNNNNNCTCC	4 cut(s)	450, 480, 1003, 1033
Bsc4I	CCNNNNNNNGG	7 cut(s)	342, 644, 645, 841, 939, 993, 1444
Bse118I	RCCGGY	2 cut(s)	265, 370
Bse1I	ACTGG	2 cut(s)	1559, 1609
Bse3DI	GCAATG	3 cut(s)	676, 1184, 2031
BseBI	CCWGG	4 cut(s)	44, 218, 534, 810
BseCI	ATCGAT	1 cut(s)	916
BseDI	CCNNGG	7 cut(s)	42, 147, 760, 809, 933, 1351, 1848
BseGI	GGATG	4 cut(s)	60, 943, 1621, 1761
BseLI	CCNNNNNNNGG	7 cut(s)	342, 644, 645, 841, 939, 993, 1444
BseMI	GCAATG	3 cut(s)	676, 1184, 2031
BseMII	CTCAG	3 cut(s)	134, 1534, 1934
BseNI	ACTGG	2 cut(s)	1559, 1609
BseX3I	CGGCCG	1 cut(s)	248
BseXI	GCAGC	7 cut(s)	136, 189, 322, 417, 661, 970, 1804
BseYI	CCCAGC	1 cut(s)	1548
BsgI	GTGCAG	1 cut(s)	461
Bsh1285I	CGRYCG	1 cut(s)	251
BshFI	GGCC	7 cut(s)	47, 250, 335, 346, 398, 834, 1350
BshVI	ATCGAT	1 cut(s)	916
BsiEI	CGRYCG	1 cut(s)	251
BsiHKAI	GWGCWC	1 cut(s)	1437
BsiHKCI	CYCGRG	3 cut(s)	422, 933, 1812
BsiSI	CCGG	7 cut(s)	208, 251, 266, 336, 371, 880, 934
BslFI	GGGAC	5 cut(s)	1126, 1436, 1507, 1531, 1729
BslI	CCNNNNNNNGG	7 cut(s)	342, 644, 645, 841, 939, 993, 1444
BsmAI	GTCTC	3 cut(s)	423, 934, 1242
BsmFI	GGGAC	5 cut(s)	1126, 1436, 1507, 1531, 1729
BsmI	GAATGC	1 cut(s)	1675
BsnI	GGCC	7 cut(s)	47, 250, 335, 346, 398, 834, 1350
BsoBI	CYCGRG	3 cut(s)	422, 933, 1812
Bsp1286I	GDGCHC	3 cut(s)	1287, 1437, 2017
Bsp143I	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
Bsp1720I	GCTNAGC	2 cut(s)	120, 708
Bsp19I	CCATGG	2 cut(s)	760, 1351
BspACI	CCGC	9 cut(s)	29, 55, 58, 117, 152, 247, 1056, 1372, 1491
BspANI	GGCC	7 cut(s)	47, 250, 335, 346, 398, 834, 1350
BspCNI	CTCAG	3 cut(s)	133, 1533, 1935
BspDI	ATCGAT	1 cut(s)	916
BspHI	TCATGA	2 cut(s)	727, 1240
BspLI	GGNNCC	1 cut(s)	1876
BspMAI	CTGCAG	1 cut(s)	1334
BspPI	GGATC	5 cut(s)	14, 347, 599, 1592, 1796
BsrBI	CCGCTC	1 cut(s)	119
BsrDI	GCAATG	3 cut(s)	676, 1184, 2031
BsrFI	RCCGGY	2 cut(s)	265, 370
BsrI	ACTGG	2 cut(s)	1559, 1609
BssAI	RCCGGY	2 cut(s)	265, 370
BssECI	CCNNGG	7 cut(s)	42, 147, 760, 809, 933, 1351, 1848
BssMI	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
BssNI	GRCGYC	2 cut(s)	263, 1263
BssSI	CACGAG	2 cut(s)	172, 897
BssT1I	CCWWGG	4 cut(s)	147, 760, 1351, 1848
Bst2BI	CACGAG	2 cut(s)	172, 897
Bst2UI	CCWGG	4 cut(s)	44, 218, 534, 810
Bst4CI	ACNGT	5 cut(s)	166, 592, 700, 1432, 1504
Bst6I	CTCTTC	1 cut(s)	810
BstACI	GRCGYC	2 cut(s)	263, 1263
BstC8I	GCNNGC	2 cut(s)	1170, 1673
BstDEI	CTNAG	9 cut(s)	120, 128, 375, 475, 708, 1520, 1602, 1914, 1943
BstDSI	CCRYGG	2 cut(s)	760, 1351
BstENI	CCTNNNNNAGG	1 cut(s)	1442
BstF5I	GGATG	4 cut(s)	60, 943, 1621, 1761
BstHHI	GCGC	3 cut(s)	146, 1671, 1857
BstKTI	GATC	7 cut(s)	22, 342, 607, 1459, 1600, 1804, 1840
BstMAI	GTCTC	3 cut(s)	423, 934, 1242
BstMBI	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
BstMCI	CGRYCG	1 cut(s)	251
BstMWI	GCNNNNNNNGC	9 cut(s)	130, 152, 253, 262, 718, 1064, 1310, 1380, 2021
BstNI	CCWGG	4 cut(s)	44, 218, 534, 810
BstNSI	RCATGY	1 cut(s)	418
BstSCI	CCNGG	8 cut(s)	42, 207, 216, 335, 532, 808, 932, 933
BstSFI	CTRYAG	1 cut(s)	1330
BstV1I	GCAGC	7 cut(s)	136, 189, 322, 417, 661, 970, 1804
BstV2I	GAAGAC	2 cut(s)	575, 1590
BstX2I	RGATCY	3 cut(s)	19, 1597, 1801
BstYI	RGATCY	3 cut(s)	19, 1597, 1801
BstZI	CGGCCG	1 cut(s)	248
Bsu15I	ATCGAT	1 cut(s)	916
BsuI	GTATCC	1 cut(s)	540
BsuRI	GGCC	7 cut(s)	47, 250, 335, 346, 398, 834, 1350
BsuTUI	ATCGAT	1 cut(s)	916
BtgI	CCRYGG	2 cut(s)	760, 1351
BtrI	CACGTC	1 cut(s)	1200
BtsCI	GGATG	4 cut(s)	60, 943, 1621, 1761
BtsIMutI	CAGTG	4 cut(s)	597, 891, 1437, 1566
Cac8I	GCNNGC	2 cut(s)	1170, 1673
CaiI	CAGNNNCTG	1 cut(s)	1565
CciI	TCATGA	2 cut(s)	727, 1240
CfoI	GCGC	3 cut(s)	146, 1671, 1857
Cfr10I	RCCGGY	2 cut(s)	265, 370
Cfr13I	GGNCC	1 cut(s)	1100
Cfr9I	CCCGGG	1 cut(s)	933
ClaI	ATCGAT	1 cut(s)	916
CseI	GACGC	3 cut(s)	271, 397, 1991
Csp6I	GTAC	5 cut(s)	355, 379, 445, 467, 1939
CviQI	GTAC	5 cut(s)	355, 379, 445, 467, 1939
DdeI	CTNAG	9 cut(s)	120, 128, 375, 475, 708, 1520, 1602, 1914, 1943
DpnI	GATC	7 cut(s)	21, 341, 606, 1458, 1599, 1803, 1839
DpnII	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
DraIII	CACNNNGTG	1 cut(s)	841
EaeI	YGGCCR	2 cut(s)	248, 832
EagI	CGGCCG	1 cut(s)	248
Eam1104I	CTCTTC	1 cut(s)	810
EarI	CTCTTC	1 cut(s)	810
EciI	GGCGGA	1 cut(s)	44
EclXI	CGGCCG	1 cut(s)	248
Eco130I	CCWWGG	4 cut(s)	147, 760, 1351, 1848
Eco147I	AGGCCT	1 cut(s)	346
Eco24I	GRGCYC	2 cut(s)	1287, 2017
Eco32I	GATATC	1 cut(s)	101
Eco47I	GGWCC	1 cut(s)	1100
Eco52I	CGGCCG	1 cut(s)	248
Eco57I	CTGAAG	3 cut(s)	242, 1017, 1137
Eco88I	CYCGRG	3 cut(s)	422, 933, 1812
EcoNI	CCTNNNNNAGG	1 cut(s)	1442
EcoRII	CCWGG	4 cut(s)	42, 216, 532, 808
EcoRV	GATATC	1 cut(s)	101
EcoT14I	CCWWGG	4 cut(s)	147, 760, 1351, 1848
EcoT22I	ATGCAT	1 cut(s)	1186
EcoT38I	GRGCYC	2 cut(s)	1287, 2017
ErhI	CCWWGG	4 cut(s)	147, 760, 1351, 1848
FaqI	GGGAC	5 cut(s)	1126, 1436, 1507, 1531, 1729
FauI	CCCGC	1 cut(s)	1484
FblI	GTMKAC	3 cut(s)	411, 858, 1980
FokI	GGATG	4 cut(s)	47, 950, 1628, 1748
FriOI	GRGCYC	2 cut(s)	1287, 2017
FspBI	CTAG	1 cut(s)	992
FspI	TGCGCA	1 cut(s)	1670
GlaI	GCGC	3 cut(s)	145, 1670, 1856
GsaI	CCCAGC	1 cut(s)	1552
GsuI	CTGGAG	1 cut(s)	545
HaeIII	GGCC	7 cut(s)	47, 250, 335, 346, 398, 834, 1350
HapII	CCGG	7 cut(s)	208, 251, 266, 336, 371, 880, 934
HgaI	GACGC	3 cut(s)	271, 397, 1991
HhaI	GCGC	3 cut(s)	146, 1671, 1857
Hin1I	GRCGYC	2 cut(s)	263, 1263
Hin6I	GCGC	3 cut(s)	144, 1669, 1855
HinP1I	GCGC	3 cut(s)	144, 1669, 1855
HincII	GTYRAC	2 cut(s)	412, 1369
HindII	GTYRAC	2 cut(s)	412, 1369
HindIII	AAGCTT	1 cut(s)	1338
HpaII	CCGG	7 cut(s)	208, 251, 266, 336, 371, 880, 934
HphI	GGTGA	6 cut(s)	86, 337, 1271, 1466, 1715, 1966
Hpy166II	GTNNAC	6 cut(s)	11, 169, 412, 859, 1369, 1981
Hpy8I	GTNNAC	6 cut(s)	11, 169, 412, 859, 1369, 1981
Hpy99I	CGWCG	3 cut(s)	362, 413, 1204
HpyAV	CCTTC	3 cut(s)	274, 1113, 1412
HpyCH4III	ACNGT	5 cut(s)	166, 592, 700, 1432, 1504
HpyCH4IV	ACGT	4 cut(s)	357, 1199, 1263, 1412
HpyF10VI	GCNNNNNNNGC	9 cut(s)	130, 152, 253, 262, 718, 1064, 1310, 1380, 2021
HpyF3I	CTNAG	9 cut(s)	120, 128, 375, 475, 708, 1520, 1602, 1914, 1943
HpySE526I	ACGT	4 cut(s)	357, 1199, 1263, 1412
Hsp92I	GRCGYC	2 cut(s)	263, 1263
HspAI	GCGC	3 cut(s)	144, 1669, 1855
Kzo9I	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
LmnI	GCTCC	6 cut(s)	253, 295, 866, 1440, 1512, 1852
Lsp1109I	GCAGC	7 cut(s)	136, 189, 322, 417, 661, 970, 1804
LweI	GCATC	5 cut(s)	69, 568, 1606, 1703, 2026
MaeI	CTAG	1 cut(s)	992
MaeII	ACGT	4 cut(s)	357, 1199, 1263, 1412
MaeIII	GTNAC	5 cut(s)	1259, 1297, 1504, 1561, 2005
MalI	GATC	7 cut(s)	21, 341, 606, 1458, 1599, 1803, 1839
MbiI	CCGCTC	1 cut(s)	119
MboI	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
MboII	GAAGA	5 cut(s)	575, 797, 899, 1023, 1595
MfeI	CAATTG	2 cut(s)	1062, 1250
MflI	RGATCY	3 cut(s)	19, 1597, 1801
MhlI	GDGCHC	3 cut(s)	1287, 1437, 2017
MlsI	TGGCCA	1 cut(s)	834
MluCI	AATT	5 cut(s)	620, 1062, 1250, 1481, 1920
MluNI	TGGCCA	1 cut(s)	834
MlyI	GAGTC	6 cut(s)	16, 718, 1535, 1726, 1751, 1763
MmeI	TCCRAC	5 cut(s)	47, 88, 210, 1593, 2044
Mox20I	TGGCCA	1 cut(s)	834
Mph1103I	ATGCAT	1 cut(s)	1186
MscI	TGGCCA	1 cut(s)	834
MseI	TTAA	6 cut(s)	429, 471, 872, 912, 1157, 1718
MslI	CAYNNNNRTG	1 cut(s)	1356
Msp20I	TGGCCA	1 cut(s)	834
MspI	CCGG	7 cut(s)	208, 251, 266, 336, 371, 880, 934
MspR9I	CCNGG	8 cut(s)	44, 209, 218, 337, 534, 810, 934, 935
MunI	CAATTG	2 cut(s)	1062, 1250
Mva1269I	GAATGC	1 cut(s)	1675
MvaI	CCWGG	4 cut(s)	44, 218, 534, 810
MwoI	GCNNNNNNNGC	9 cut(s)	130, 152, 253, 262, 718, 1064, 1310, 1380, 2021
NciI	CCSGG	4 cut(s)	209, 337, 934, 935
NcoI	CCATGG	2 cut(s)	760, 1351
NdeII	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
NlaIV	GGNNCC	1 cut(s)	1876
NmuCI	GTSAC	4 cut(s)	1259, 1297, 1504, 1561
NsbI	TGCGCA	1 cut(s)	1670
NsiI	ATGCAT	1 cut(s)	1186
NspI	RCATGY	1 cut(s)	418
PaeR7I	CTCGAG	1 cut(s)	1812
PagI	TCATGA	2 cut(s)	727, 1240
PceI	AGGCCT	1 cut(s)	346
PciI	ACATGT	1 cut(s)	414
PcsI	WCGNNNNNNNCGW	1 cut(s)	170
PctI	GAATGC	1 cut(s)	1675
PfeI	GAWTC	4 cut(s)	38, 876, 891, 918
PflMI	CCANNNNNTGG	1 cut(s)	841
PfoI	TCCNGGA	1 cut(s)	216
PleI	GAGTC	6 cut(s)	15, 718, 1534, 1726, 1750, 1762
PpsI	GAGTC	6 cut(s)	15, 718, 1534, 1726, 1750, 1762
PscI	ACATGT	1 cut(s)	414
PsiI	TTATAA	1 cut(s)	626
Psp6I	CCWGG	4 cut(s)	42, 216, 532, 808
PspFI	CCCAGC	1 cut(s)	1548
PspGI	CCWGG	4 cut(s)	42, 216, 532, 808
PspN4I	GGNNCC	1 cut(s)	1876
PspPI	GGNCC	1 cut(s)	1100
PstI	CTGCAG	1 cut(s)	1334
PstNI	CAGNNNCTG	1 cut(s)	1565
PsuI	RGATCY	3 cut(s)	19, 1597, 1801
RsaI	GTAC	5 cut(s)	356, 380, 446, 468, 1940
RsaNI	GTAC	5 cut(s)	355, 379, 445, 467, 1939
RseI	CAYNNNNRTG	1 cut(s)	1356
SalI	GTCGAC	1 cut(s)	410
SaqAI	TTAA	6 cut(s)	429, 471, 872, 912, 1157, 1718
Sau3AI	GATC	7 cut(s)	19, 339, 604, 1456, 1597, 1801, 1837
Sau96I	GGNCC	1 cut(s)	1100
ScaI	AGTACT	1 cut(s)	380
SchI	GAGTC	6 cut(s)	16, 718, 1535, 1726, 1751, 1763
ScrFI	CCNGG	8 cut(s)	44, 209, 218, 337, 534, 810, 934, 935
SduI	GDGCHC	3 cut(s)	1287, 1437, 2017
SfaNI	GCATC	5 cut(s)	69, 568, 1606, 1703, 2026
SfcI	CTRYAG	1 cut(s)	1330
Sfr274I	CTCGAG	1 cut(s)	1812
SgrAI	CRCCGGYG	1 cut(s)	265
SinI	GGWCC	1 cut(s)	1100
SlaI	CTCGAG	1 cut(s)	1812
SmaI	CCCGGG	1 cut(s)	935
SmiMI	CAYNNNNRTG	1 cut(s)	1356
SmlI	CTYRAG	4 cut(s)	1046, 1286, 1384, 1812
SmoI	CTYRAG	4 cut(s)	1046, 1286, 1384, 1812
Sse9I	AATT	5 cut(s)	620, 1062, 1250, 1481, 1920
SseBI	AGGCCT	1 cut(s)	346
SsiI	CCGC	9 cut(s)	29, 55, 58, 117, 152, 247, 1056, 1372, 1491
SspI	AATATT	1 cut(s)	1220
SspMI	CTAG	1 cut(s)	992
StuI	AGGCCT	1 cut(s)	346
StyD4I	CCNGG	8 cut(s)	42, 207, 216, 335, 532, 808, 932, 933
StyI	CCWWGG	4 cut(s)	147, 760, 1351, 1848
TaaI	ACNGT	5 cut(s)	166, 592, 700, 1432, 1504
TaiI	ACGT	4 cut(s)	360, 1202, 1266, 1415
TaqI	TCGA	7 cut(s)	411, 516, 630, 916, 1570, 1639, 1813
TaqII	GACCGA	1 cut(s)	1007
TasI	AATT	5 cut(s)	620, 1062, 1250, 1481, 1920
TatI	WGTACW	3 cut(s)	378, 444, 466
TauI	GCSGC	5 cut(s)	57, 60, 155, 250, 1058
TfiI	GAWTC	4 cut(s)	38, 876, 891, 918
Tru1I	TTAA	6 cut(s)	429, 471, 872, 912, 1157, 1718
Tru9I	TTAA	6 cut(s)	429, 471, 872, 912, 1157, 1718
TscAI	CASTG	4 cut(s)	597, 891, 1437, 1566
TseFI	GTSAC	4 cut(s)	1259, 1297, 1504, 1561
TseI	GCWGC	7 cut(s)	124, 177, 310, 405, 674, 983, 1817
Tsp45I	GTSAC	4 cut(s)	1259, 1297, 1504, 1561
TspDTI	ATGAA	8 cut(s)	320, 381, 633, 671, 757, 1244, 1260, 1547
TspMI	CCCGGG	1 cut(s)	933
TspRI	CASTG	4 cut(s)	597, 891, 1437, 1566
Van91I	CCANNNNNTGG	1 cut(s)	841
VpaK11BI	GGWCC	1 cut(s)	1100
XagI	CCTNNNNNAGG	1 cut(s)	1442
XapI	RAATTY	2 cut(s)	620, 1481
XceI	RCATGY	1 cut(s)	418
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1204
XhoI	CTCGAG	1 cut(s)	1812
XmaI	CCCGGG	1 cut(s)	933
XmiI	GTMKAC	3 cut(s)	411, 858, 1980
XspI	CTAG	1 cut(s)	992
ZraI	GACGTC	1 cut(s)	1264
ZrmI	AGTACT	1 cut(s)	380
Zsp2I	ATGCAT	1 cut(s)	1186

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.