Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0012504

Physical Location & Seq

Genomic Coordinates Forward (+)

27336370 .. 27343069

Gene Structure 6700 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG256300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGGCAACATTTGATCCGATCACAGATGAGTTTGTAATTGACACACCAAATGATGGGGCCATCAAATGGTGGATTGGCAATGCTGCTGTTCATGGCAAGTTTGCCACTGTTTTTGCTAAGCTGATGTTGCCGACACATGATGAAAGAGGAGTATCTGATATGGGTGTCCACGCCTTCATTGTACCAATAAGGGATATGAAGACTCATCAAACTCTTCCAGGAATTGAGATAAACGATTGTGGCCATAAGGTTGGTCTGAATGGGGTGGACAATGGAGCATTAAGATTCCGCGCAGTGAGAATCCCTCGTGATAATCTTCTTAATCGCTTTGGAGATGTCTCTCGGGATGGTAAATACACAAGTAGCTTACCATCTATAAATAAAAGATTTGCTGCCACACTAGGAGAACTTGTAGGTGGGAGGGTCGGCCTTGCATATTCTTCAGGCAGTGTCTTGAAGGTTGCTGGCACAATTGCAATCCGATATTCTCTACTGCGTCAGCAGTTTGGTCCTCCAAAGCAACCTGAAGTCAGTATTCTTGATTACCAATCCCAGCAGCACAAGTTGATGCCAATGCTGGCTTCAACTTACGCATTCCATTTTGCCACACTACATTTGGTGGAGAAATATTCAGAGATGAAGAAGACTCATGATGAACAATTGGTTGCAGATGTCCATTCTCTTTCAGCAGGGCTCAAGGCTTATGTGACGTCATATACAGCAAAGTCATTGAGTATCTGTAGGGAAGCTTGTGGAGGCCATGGGTACGCTGCTGTCAACCGCTTTGGCAGTTTGAGGAATGACCATGACATTTTTCAGACATTCGAAGGGGACAACACGGTGCTTCTACAGCAGGTAGCAGGTGATCTGTTGAAGCAATACAAGGAGAAGTTTCAAGGCGGGACACTCACAGTTACCTGGAATTACCTTAGAGAATCCATGAACTCATACCTATCTCAGCCAAATCCAGTGACTGCTCGATGGGAAAGTGAAGACCATTTGCGAGATCCAAAGTTCCAGTTGGATGCTTTCAGATACCGAACTTCTCGACTACTTCAAAGTGTTGCTGTTAGACTTCGCAAGCACTCTAAAACTCTTGGAAGTTTTGGCGCTTGGAATAGATGCTTAAACCACCTTTTGACACTTGCAGAGTCCCATATTGAATCTGTCATCCTTGCCAAGTTTATAGAAGCTGTGCAGAACTGTCCAGATGCAAGTTCTCGAGCAGCTTTGAAACTCGTCTGTGATCTTTATGCCTTGGATCAAATATGGAAAGACATAGGAACCTATCGTAATGTTGATTATGTTGCACCAAACAAAGCTAAGGCAATCCACAAGCTCACTGCATACCTGAGTTTCCGAGTGAGAAATATAGCAGGGGAACTCATAGACGCATTTGATATTCCAGATTATGTTACGCGTGCCCCAATTGCAATGCAGTCAGATGCCTATTCTCAGTACACACAGCATGTGGGATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042579 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071704 GO:0072329 GO:0097159 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_1 Acyl-CoA_dh_M

Protein Analysis

493

Amino Acids

55.12

Weight (kDa)

8.89

Isoelectric Point (pI)

35.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_1	PF00441	132 - 288	1.7e-09	Acyl-CoA dehydrogenase, C-terminal domain
ACOX_C_alpha1	PF22924	135 - 290	1.5e-50	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	336 - 489	1.2e-24	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65110 AT5G65110
fragaria_vesca	FvH4_1g21250
malus_domestica	MD01G1026400.v1.1 MD15G1327100.v1.1
prunus_persica	Prupe.6G181800_v2.0.a1
pyrus_communis	pycom01g05460 pycom395g00040
rosa_chinensis	RchiOBHm_Chr2g0115091
rosa_laevigata	RLG00000018156
rosa_multiflora	Rmu_sc0002784.1_g000002
rosa_roxburghii	Rroxscaffold_2G00130560
rosa_rugosa	Rorug02G0193300 Rorug02G0193400
rosa_samantha	Rh2AG251800 Rh2BG261400 Rh2CG256300 Rh2DG259900
rosa_wichuraiana	Rw2G019640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	851
AatII	GACGTC	1 cut(s)	713
Acc36I	ACCTGC	2 cut(s)	844, 851
AccB7I	CCANNNNNTGG	2 cut(s)	53, 66
AccII	CGCG	2 cut(s)	291, 1420
AciI	CCGC	3 cut(s)	289, 781, 900
AclWI	GGATC	3 cut(s)	8, 1001, 1269
AcoI	YGGCCR	1 cut(s)	241
AcuI	CTGAAG	2 cut(s)	426, 546
AcyI	GRCGYC	1 cut(s)	710
AfaI	GTAC	3 cut(s)	183, 767, 1460
AfiI	CCNNNNNNNGG	2 cut(s)	53, 66
AflIII	ACRYGT	1 cut(s)	1418
AgsI	TTSAA	7 cut(s)	457, 585, 874, 896, 1058, 1163, 1234
AjnI	CCWGG	2 cut(s)	217, 917
AjuI	GAANNNNNNNTTGG	2 cut(s)	565, 597
AluBI	AGCT	7 cut(s)	121, 366, 749, 1193, 1229, 1322, 1339
AluI	AGCT	7 cut(s)	121, 366, 749, 1193, 1229, 1322, 1339
Alw26I	GTCTC	1 cut(s)	343
AlwI	GGATC	3 cut(s)	8, 1001, 1269
AlwNI	CAGNNNCTG	1 cut(s)	974
Ama87I	CYCGRG	2 cut(s)	342, 1221
AoxI	GGCC	4 cut(s)	57, 241, 427, 757
ApeKI	GCWGC	5 cut(s)	83, 392, 556, 770, 1226
AspLEI	GCGC	2 cut(s)	293, 1112
AspS9I	GGNCC	2 cut(s)	57, 509
AsuHPI	GGTGA	1 cut(s)	875
AsuII	TTCGAA	1 cut(s)	825
AvaI	CYCGRG	2 cut(s)	342, 1221
AvaII	GGWCC	1 cut(s)	509
BaeGI	GKGCMC	1 cut(s)	1426
BalI	TGGCCA	1 cut(s)	243
BanII	GRGCYC	1 cut(s)	696
BauI	CACGAG	1 cut(s)	306
BbsI	GAAGAC	3 cut(s)	206, 650, 999
BbvI	GCAGC	5 cut(s)	70, 379, 568, 757, 1238
BccI	CCATC	5 cut(s)	47, 68, 341, 379, 975
BciT130I	CCWGG	2 cut(s)	219, 919
BcoDI	GTCTC	1 cut(s)	343
BfaI	CTAG	1 cut(s)	401
BfmI	CTRYAG	2 cut(s)	739, 848
BfoI	RGCGCY	1 cut(s)	1113
BfuAI	ACCTGC	2 cut(s)	844, 851
BisI	GCNGC	5 cut(s)	84, 393, 557, 771, 1227
BlpI	GCTNAGC	1 cut(s)	117
BlsI	GCNGC	5 cut(s)	85, 394, 558, 772, 1228
Bme1390I	CCNGG	2 cut(s)	219, 919
Bme18I	GGWCC	1 cut(s)	509
BmeT110I	CYCGRG	2 cut(s)	342, 1221
BmgT120I	GGNCC	2 cut(s)	57, 509
BmiI	GGNNCC	2 cut(s)	58, 1285
BmrFI	CCNGG	2 cut(s)	219, 919
BmsI	GCATC	5 cut(s)	558, 1015, 1112, 1201, 1435
BpiI	GAAGAC	3 cut(s)	206, 650, 999
BplI	GAGNNNNNCTC	2 cut(s)	289, 321
Bpu10I	CCTNAGC	1 cut(s)	1323
Bpu1102I	GCTNAGC	1 cut(s)	117
Bpu14I	TTCGAA	1 cut(s)	825
BpuEI	CTTGAG	1 cut(s)	680
BsaHI	GRCGYC	1 cut(s)	710
BsaJI	CCNNGG	2 cut(s)	760, 1257
BsaXI	ACNNNNNCTCC	2 cut(s)	396, 426
Bsc4I	CCNNNNNNNGG	2 cut(s)	53, 66
Bse1I	ACTGG	2 cut(s)	968, 1018
Bse3DI	GCAATG	2 cut(s)	85, 1440
BseBI	CCWGG	2 cut(s)	219, 919
BseDI	CCNNGG	2 cut(s)	760, 1257
BseGI	GGATG	3 cut(s)	352, 1030, 1170
BseLI	CCNNNNNNNGG	2 cut(s)	53, 66
BseMI	GCAATG	2 cut(s)	85, 1440
BseMII	CTCAG	3 cut(s)	971, 1343, 1469
BseNI	ACTGG	2 cut(s)	968, 1018
BseRI	GAGGAG	1 cut(s)	162
BseSI	GKGCMC	1 cut(s)	1426
BseXI	GCAGC	5 cut(s)	70, 379, 568, 757, 1238
BseYI	CCCAGC	1 cut(s)	552
BsgI	GTGCAG	1 cut(s)	1217
Bsh1236I	CGCG	2 cut(s)	291, 1420
BshFI	GGCC	4 cut(s)	59, 243, 429, 759
BsiHKCI	CYCGRG	2 cut(s)	342, 1221
BslFI	GGGAC	3 cut(s)	845, 916, 1138
BslI	CCNNNNNNNGG	2 cut(s)	53, 66
BsmAI	GTCTC	1 cut(s)	343
BsmFI	GGGAC	3 cut(s)	845, 916, 1138
BsmI	GAATGC	1 cut(s)	593
BsnI	GGCC	4 cut(s)	59, 243, 429, 759
BsoBI	CYCGRG	2 cut(s)	342, 1221
Bsp119I	TTCGAA	1 cut(s)	825
Bsp1286I	GDGCHC	2 cut(s)	696, 1426
Bsp143I	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
Bsp1720I	GCTNAGC	1 cut(s)	117
Bsp19I	CCATGG	1 cut(s)	760
BspACI	CCGC	3 cut(s)	289, 781, 900
BspANI	GGCC	4 cut(s)	59, 243, 429, 759
BspCNI	CTCAG	3 cut(s)	970, 1344, 1468
BspFNI	CGCG	2 cut(s)	291, 1420
BspHI	TCATGA	1 cut(s)	649
BspLI	GGNNCC	2 cut(s)	58, 1285
BspMI	ACCTGC	2 cut(s)	844, 851
BspPI	GGATC	3 cut(s)	8, 1001, 1269
BspT104I	TTCGAA	1 cut(s)	825
BsrDI	GCAATG	2 cut(s)	85, 1440
BsrI	ACTGG	2 cut(s)	968, 1018
BssECI	CCNNGG	2 cut(s)	760, 1257
BssMI	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
BssNI	GRCGYC	1 cut(s)	710
BssSI	CACGAG	1 cut(s)	306
BssT1I	CCWWGG	2 cut(s)	760, 1257
Bst2BI	CACGAG	1 cut(s)	306
Bst2UI	CCWGG	2 cut(s)	219, 919
Bst4CI	ACNGT	4 cut(s)	109, 841, 913, 1205
Bst6I	CTCTTC	1 cut(s)	219
BstACI	GRCGYC	1 cut(s)	710
BstBI	TTCGAA	1 cut(s)	825
BstC8I	GCNNGC	4 cut(s)	466, 579, 1082, 1422
BstDEI	CTNAG	6 cut(s)	117, 929, 957, 1323, 1352, 1455
BstDSI	CCRYGG	1 cut(s)	760
BstF5I	GGATG	3 cut(s)	352, 1030, 1170
BstFNI	CGCG	2 cut(s)	291, 1420
BstH2I	RGCGCY	1 cut(s)	1113
BstHHI	GCGC	2 cut(s)	293, 1112
BstKTI	GATC	6 cut(s)	16, 21, 868, 1009, 1249, 1264
BstMAI	GTCTC	1 cut(s)	343
BstMBI	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
BstMWI	GCNNNNNNNGC	3 cut(s)	127, 850, 1430
BstNI	CCWGG	2 cut(s)	219, 919
BstNSI	RCATGY	1 cut(s)	1472
BstSCI	CCNGG	2 cut(s)	217, 917
BstSFI	CTRYAG	2 cut(s)	739, 848
BstSLI	GKGCMC	1 cut(s)	1426
BstUI	CGCG	2 cut(s)	291, 1420
BstV1I	GCAGC	5 cut(s)	70, 379, 568, 757, 1238
BstV2I	GAAGAC	3 cut(s)	206, 650, 999
BstX2I	RGATCY	1 cut(s)	1006
BstXI	CCANNNNNNTGG	1 cut(s)	251
BstYI	RGATCY	1 cut(s)	1006
BsuRI	GGCC	4 cut(s)	59, 243, 429, 759
BtgI	CCRYGG	1 cut(s)	760
BtsCI	GGATG	3 cut(s)	352, 1030, 1170
BtsI	GCAGTG	3 cut(s)	300, 454, 1341
BtsIMutI	CAGTG	5 cut(s)	105, 300, 454, 975, 1341
BveI	ACCTGC	2 cut(s)	844, 851
Cac8I	GCNNGC	4 cut(s)	466, 579, 1082, 1422
CaiI	CAGNNNCTG	1 cut(s)	974
CciI	TCATGA	1 cut(s)	649
CfoI	GCGC	2 cut(s)	293, 1112
Cfr13I	GGNCC	2 cut(s)	57, 509
CseI	GACGC	2 cut(s)	485, 1400
Csp6I	GTAC	3 cut(s)	182, 766, 1459
CspCI	CAANNNNNGTGG	2 cut(s)	94, 129
CviAII	CATG	7 cut(s)	92, 137, 650, 761, 806, 940, 1469
CviQI	GTAC	3 cut(s)	182, 766, 1459
DdeI	CTNAG	6 cut(s)	117, 929, 957, 1323, 1352, 1455
DpnI	GATC	6 cut(s)	15, 20, 867, 1008, 1248, 1263
DpnII	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
EaeI	YGGCCR	1 cut(s)	241
Eam1104I	CTCTTC	1 cut(s)	219
EarI	CTCTTC	1 cut(s)	219
Eco130I	CCWWGG	2 cut(s)	760, 1257
Eco24I	GRGCYC	1 cut(s)	696
Eco47I	GGWCC	1 cut(s)	509
Eco57I	CTGAAG	2 cut(s)	426, 546
Eco88I	CYCGRG	2 cut(s)	342, 1221
EcoRII	CCWGG	2 cut(s)	217, 917
EcoT14I	CCWWGG	2 cut(s)	760, 1257
EcoT38I	GRGCYC	1 cut(s)	696
ErhI	CCWWGG	2 cut(s)	760, 1257
FaeI	CATG	7 cut(s)	95, 140, 653, 764, 809, 943, 1472
FaqI	GGGAC	3 cut(s)	845, 916, 1138
FatI	CATG	7 cut(s)	91, 136, 649, 760, 805, 939, 1468
FauI	CCCGC	1 cut(s)	893
Fnu4HI	GCNGC	5 cut(s)	84, 393, 557, 771, 1227
FokI	GGATG	3 cut(s)	359, 1037, 1157
FriOI	GRGCYC	1 cut(s)	696
Fsp4HI	GCNGC	5 cut(s)	84, 393, 557, 771, 1227
FspBI	CTAG	1 cut(s)	401
GlaI	GCGC	2 cut(s)	292, 1111
GluI	GCNGC	5 cut(s)	84, 393, 557, 771, 1227
GsaI	CCCAGC	1 cut(s)	556
HaeII	RGCGCY	1 cut(s)	1113
HaeIII	GGCC	4 cut(s)	59, 243, 429, 759
HgaI	GACGC	2 cut(s)	485, 1400
HhaI	GCGC	2 cut(s)	293, 1112
Hin1I	GRCGYC	1 cut(s)	710
Hin1II	CATG	7 cut(s)	95, 140, 653, 764, 809, 943, 1472
Hin6I	GCGC	2 cut(s)	291, 1110
HinP1I	GCGC	2 cut(s)	291, 1110
HincII	GTYRAC	1 cut(s)	778
HindII	GTYRAC	1 cut(s)	778
HindIII	AAGCTT	1 cut(s)	747
HinfI	GANTC	7 cut(s)	202, 285, 300, 646, 935, 1151, 1163
HphI	GGTGA	1 cut(s)	875
Hpy166II	GTNNAC	4 cut(s)	169, 268, 778, 1461
Hpy188I	TCNGA	9 cut(s)	18, 157, 258, 482, 634, 819, 1034, 1361, 1444
Hpy188III	TCNNGA	9 cut(s)	308, 344, 454, 539, 650, 1047, 1208, 1221, 1406
Hpy8I	GTNNAC	4 cut(s)	169, 268, 778, 1461
HpyAV	CCTTC	3 cut(s)	184, 451, 821
HpyCH4III	ACNGT	4 cut(s)	109, 841, 913, 1205
HpyCH4IV	ACGT	1 cut(s)	710
HpyF10VI	GCNNNNNNNGC	3 cut(s)	127, 850, 1430
HpyF3I	CTNAG	6 cut(s)	117, 929, 957, 1323, 1352, 1455
HpySE526I	ACGT	1 cut(s)	710
Hsp92I	GRCGYC	1 cut(s)	710
Hsp92II	CATG	7 cut(s)	95, 140, 653, 764, 809, 943, 1472
HspAI	GCGC	2 cut(s)	291, 1110
Kzo9I	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
LmnI	GCTCC	1 cut(s)	275
Lsp1109I	GCAGC	5 cut(s)	70, 379, 568, 757, 1238
LweI	GCATC	5 cut(s)	558, 1015, 1112, 1201, 1435
MaeI	CTAG	1 cut(s)	401
MaeII	ACGT	1 cut(s)	710
MaeIII	GTNAC	4 cut(s)	706, 913, 970, 1414
MalI	GATC	6 cut(s)	15, 20, 867, 1008, 1248, 1263
MboI	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
MboII	GAAGA	7 cut(s)	206, 211, 308, 432, 652, 655, 1004
MfeI	CAATTG	3 cut(s)	471, 659, 1428
MflI	RGATCY	1 cut(s)	1006
MhlI	GDGCHC	2 cut(s)	696, 1426
MlsI	TGGCCA	1 cut(s)	243
MluCI	AATT	6 cut(s)	36, 222, 471, 659, 922, 1428
MluI	ACGCGT	1 cut(s)	1418
MluNI	TGGCCA	1 cut(s)	243
MlyI	GAGTC	3 cut(s)	196, 640, 1160
MmeI	TCCRAC	1 cut(s)	1002
MnlI	CCTC	6 cut(s)	140, 315, 414, 522, 749, 789
Mox20I	TGGCCA	1 cut(s)	243
MscI	TGGCCA	1 cut(s)	243
MseI	TTAA	4 cut(s)	281, 321, 1127, 1480
Msp20I	TGGCCA	1 cut(s)	243
MspR9I	CCNGG	2 cut(s)	219, 919
MunI	CAATTG	3 cut(s)	471, 659, 1428
Mva1269I	GAATGC	1 cut(s)	593
MvaI	CCWGG	2 cut(s)	219, 919
MvnI	CGCG	2 cut(s)	291, 1420
MwoI	GCNNNNNNNGC	3 cut(s)	127, 850, 1430
NcoI	CCATGG	1 cut(s)	760
NdeII	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
NlaIII	CATG	7 cut(s)	95, 140, 653, 764, 809, 943, 1472
NlaIV	GGNNCC	2 cut(s)	58, 1285
NmuCI	GTSAC	2 cut(s)	706, 970
NspI	RCATGY	1 cut(s)	1472
NspV	TTCGAA	1 cut(s)	825
PaeR7I	CTCGAG	1 cut(s)	1221
PagI	TCATGA	1 cut(s)	649
PaqCI	CACCTGC	1 cut(s)	851
PctI	GAATGC	1 cut(s)	593
PfeI	GAWTC	4 cut(s)	285, 300, 935, 1163
PflMI	CCANNNNNTGG	2 cut(s)	53, 66
PfoI	TCCNGGA	1 cut(s)	217
PkrI	GCNGC	5 cut(s)	85, 394, 558, 772, 1228
PleI	GAGTC	3 cut(s)	196, 640, 1159
PpsI	GAGTC	3 cut(s)	196, 640, 1159
Psp6I	CCWGG	2 cut(s)	217, 917
PspFI	CCCAGC	1 cut(s)	552
PspGI	CCWGG	2 cut(s)	217, 917
PspN4I	GGNNCC	2 cut(s)	58, 1285
PspPI	GGNCC	2 cut(s)	57, 509
PstNI	CAGNNNCTG	1 cut(s)	974
PsuI	RGATCY	1 cut(s)	1006
RsaI	GTAC	3 cut(s)	183, 767, 1460
RsaNI	GTAC	3 cut(s)	182, 766, 1459
SaqAI	TTAA	4 cut(s)	281, 321, 1127, 1480
SatI	GCNGC	5 cut(s)	84, 393, 557, 771, 1227
Sau3AI	GATC	6 cut(s)	13, 18, 865, 1006, 1246, 1261
Sau96I	GGNCC	2 cut(s)	57, 509
SchI	GAGTC	3 cut(s)	196, 640, 1160
ScrFI	CCNGG	2 cut(s)	219, 919
SduI	GDGCHC	2 cut(s)	696, 1426
SfaNI	GCATC	5 cut(s)	558, 1015, 1112, 1201, 1435
SfcI	CTRYAG	2 cut(s)	739, 848
Sfr274I	CTCGAG	1 cut(s)	1221
SfuI	TTCGAA	1 cut(s)	825
SinI	GGWCC	1 cut(s)	509
SlaI	CTCGAG	1 cut(s)	1221
SmlI	CTYRAG	2 cut(s)	695, 1221
SmoI	CTYRAG	2 cut(s)	695, 1221
Sse9I	AATT	6 cut(s)	36, 222, 471, 659, 922, 1428
SsiI	CCGC	3 cut(s)	289, 781, 900
SspI	AATATT	1 cut(s)	629
SspMI	CTAG	1 cut(s)	401
StyD4I	CCNGG	2 cut(s)	217, 917
StyI	CCWWGG	2 cut(s)	760, 1257
TaaI	ACNGT	4 cut(s)	109, 841, 913, 1205
TaiI	ACGT	1 cut(s)	713
TaqI	TCGA	4 cut(s)	825, 979, 1048, 1222
TasI	AATT	6 cut(s)	36, 222, 471, 659, 922, 1428
TatI	WGTACW	1 cut(s)	1458
TfiI	GAWTC	4 cut(s)	285, 300, 935, 1163
Tru1I	TTAA	4 cut(s)	281, 321, 1127, 1480
Tru9I	TTAA	4 cut(s)	281, 321, 1127, 1480
TscAI	CASTG	5 cut(s)	112, 300, 454, 975, 1348
TseFI	GTSAC	2 cut(s)	706, 970
TseI	GCWGC	5 cut(s)	83, 392, 556, 770, 1226
Tsp45I	GTSAC	2 cut(s)	706, 970
TspDTI	ATGAA	7 cut(s)	80, 156, 166, 212, 653, 669, 956
TspRI	CASTG	5 cut(s)	112, 300, 454, 975, 1348
Van91I	CCANNNNNTGG	2 cut(s)	53, 66
VpaK11BI	GGWCC	1 cut(s)	509
XceI	RCATGY	1 cut(s)	1472
XcmI	CCANNNNNNNNNTGG	1 cut(s)	613
XhoI	CTCGAG	1 cut(s)	1221
XspI	CTAG	1 cut(s)	401
ZraI	GACGTC	1 cut(s)	711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.