Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0012504

Physical Location & Seq

Genomic Coordinates Forward (+)

66786104 .. 66793225

Gene Structure 7122 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00130560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGAGAGGAAAGCACAGGGAAATCCAAGATAAGGTTATGGACTACTTCAACAACAGGCCTGACCTTCAGACTCCGGTGGAGATTTTGAAGGACGATCACCGCGAGCTTTGTATGAGGCAGCTTACTGGTTTGGTTAGGGAGGCCGGAATCAGACCGTTCCGGTACGTCGTCGAAGATCCGGCCAAGTACTTTGCGGTTCTGGAAGCTGTGGGCAGCGTCGACATGTCGCTTGGGATCAAGATGGGGGTGCAGTACAGTCTTTGGGGAGGTTCTGTAATCAACTTAGGAACTAAGAAACACAAGGATAAGTACTTTGATGGTATTGACAATCTGGAGTATCCAGGTTGTTTTGCTATGACTGAACTGCATCATGGCTCAAATGTTCAAGGTCTACAAACAATGGCAACATTTGATCCGATCACAGATGAGTTTGTAATTGACACACCAAATGATGGGGCCATCAAATGGTGGATTGGCAATGCTGCTGTTCATGGCAAGTTTGCCACTGTTTTTGCTAAGCTGATGTTGCCAACACATGATAAAAGAGGAGTATCTGATATGGGTGTCCACGCCTTCATTGTACCAATAAGGGATATGAAGACTCATCAAACTCTTCCAGGAATTGAGATAAACGATTGTGGCCATAAGGTTGGTCTGAATGGGGTGGACAATGGAGCATTAAGATTCCGCGCAGTGAGAATCCCTCGTGATAATCTTCTTAATCGCTTTGGAGATGTCTCTCGGGATGGTAAATACACAAGTAGCCTACCATCTATAAATAAAAGATTTGCTGCCACACTAGGAGAACTTGTAGGTGGGAGGGTCGGCCTTGCATATTCTTCAGGCAGTGTCTTGAAGGTTGCTGGCACAATTGCAATCCGATATTCTCTACTGCGTCAGCAGTTTGGTCCTCCAAAGCAACCAGAAGTCAGTATTCTTGATTACCAATCCCAGCAGCACAAGTTAATGCCAATGCTGGCTTCAACTTACGCATTCCATTTTGCCACACTGCATTTGGTGGAGAAATATTCAGAGATGAAGAAGACTCATGATGAACAATTGGTTGCAGATGTCCATTCTCTTTCAGCAGGGCTCAAGGCTTATGTGACGTCATATACAGCAAAGTCATTGAGTATCTGTAGGGAAGCTTGTGGAGGCCATGGGTACGCTGCTGTCAACCGCTTTGGCAGTTTGAGGAATGACCATGACATTTTTCAGACATTTGAAGGGGACAACACGGTGCTTCTACAGCAGGTAGCAGGTGATCTGTTGAAGCAATACAAGGAGAAGTTTCAAGGCGGGACACTCACAGTTACCTGGAACTACCTTAGAGAATCCATGAACTCATACCTATCTCAGCCAAATCCGGTGACTGCTCGATGGGAAAGTGAAGACCATTTGCGAGATCCAAAGTTCCAGTTGGATGCTTTCAGATACCGAACTTCTCGACTACTTCAAAGTGTTGCTGTTAGACTTCGCAAGCACTCTAAAACTCTTGGAAGTTTTGGCGCTTGGAATAGATGCTTAAACCACCTTTTGACACTTGCAGAGTCCCATATTGAATCTGTCATCCTTGCCAAGTTTATAGAAGCTGTGCAGAACTGTCCGGATGCAAGTTCTCGAGCAGCTCTGAAACTCGTCTGTGATCTTTATGCCTTGGATCGAATATGGAAAGACATAGGAACCTATCGTAATGTTGATTATGTTGCACCAAACAAAGCTAAGGCAATCCACAAGCTCACCGCATACCTGAGTTTCCGAGTGAGAAATATAGCAGGGGAACTCATAGACGCATTTGATATTCCAGATTATGTTACGCGTGCCCCAATTGCAATGCAGTCAGATGCCTACTCTCAGTACACACAGCATGTGGGATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042579 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071704 GO:0072329 GO:0097159 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_1 Acyl-CoA_dh_M

Protein Analysis

626

Amino Acids

70.31

Weight (kDa)

8.89

Isoelectric Point (pI)

35.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_M	PF02770	116 - 232	6.2e-12	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	265 - 421	2.7e-09	Acyl-CoA dehydrogenase, C-terminal domain
ACOX_C_alpha1	PF22924	268 - 423	2.4e-50	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	469 - 622	4.9e-24	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65110 AT5G65110
fragaria_vesca	FvH4_1g21250
malus_domestica	MD01G1026400.v1.1 MD15G1327100.v1.1
prunus_persica	Prupe.6G181800_v2.0.a1
pyrus_communis	pycom01g05460 pycom395g00040
rosa_chinensis	RchiOBHm_Chr2g0115091
rosa_laevigata	RLG00000018156
rosa_multiflora	Rmu_sc0002784.1_g000002
rosa_roxburghii	Rroxscaffold_2G00130560
rosa_rugosa	Rorug02G0193300 Rorug02G0193400
rosa_samantha	Rh2AG251800 Rh2BG261400 Rh2CG256300 Rh2DG259900
rosa_wichuraiana	Rw2G019640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1250
AatII	GACGTC	1 cut(s)	1112
Acc36I	ACCTGC	2 cut(s)	1243, 1250
AccB7I	CCANNNNNTGG	2 cut(s)	452, 465
AccI	GTMKAC	2 cut(s)	219, 391
AccII	CGCG	3 cut(s)	102, 690, 1819
AccIII	TCCGGA	1 cut(s)	1606
AciI	CCGC	6 cut(s)	100, 194, 688, 1180, 1299, 1743
AclWI	GGATC	5 cut(s)	170, 242, 407, 1400, 1668
AcoI	YGGCCR	2 cut(s)	180, 640
AcuI	CTGAAG	2 cut(s)	50, 825
AcyI	GRCGYC	1 cut(s)	1109
AfaI	GTAC	7 cut(s)	164, 188, 254, 311, 582, 1166, 1859
AfiI	CCNNNNNNNGG	3 cut(s)	31, 452, 465
AflIII	ACRYGT	2 cut(s)	222, 1817
AjnI	CCWGG	3 cut(s)	340, 616, 1316
AjuI	GAANNNNNNNTTGG	2 cut(s)	964, 996
AluBI	AGCT	9 cut(s)	106, 121, 206, 520, 1148, 1592, 1628, 1721, 1738
AluI	AGCT	9 cut(s)	106, 121, 206, 520, 1148, 1592, 1628, 1721, 1738
Alw26I	GTCTC	1 cut(s)	742
AlwI	GGATC	5 cut(s)	170, 242, 407, 1400, 1668
Ama87I	CYCGRG	2 cut(s)	741, 1620
Aor13HI	TCCGGA	1 cut(s)	1606
AoxI	GGCC	7 cut(s)	56, 141, 180, 456, 640, 826, 1156
ApeKI	GCWGC	7 cut(s)	118, 213, 482, 791, 955, 1169, 1625
AspLEI	GCGC	2 cut(s)	692, 1511
AspS9I	GGNCC	2 cut(s)	456, 908
AsuHPI	GGTGA	4 cut(s)	89, 1274, 1381, 1732
AvaI	CYCGRG	2 cut(s)	741, 1620
AvaII	GGWCC	1 cut(s)	908
BaeGI	GKGCMC	1 cut(s)	1825
BalI	TGGCCA	1 cut(s)	642
BanII	GRGCYC	1 cut(s)	1095
BauI	CACGAG	1 cut(s)	705
BbsI	GAAGAC	3 cut(s)	605, 1049, 1398
BbvI	GCAGC	7 cut(s)	130, 225, 469, 778, 967, 1156, 1637
BccI	CCATC	7 cut(s)	235, 311, 446, 467, 740, 778, 1374
BciT130I	CCWGG	3 cut(s)	342, 618, 1318
BciVI	GTATCC	1 cut(s)	348
BcoDI	GTCTC	1 cut(s)	742
BfaI	CTAG	1 cut(s)	800
BfmI	CTRYAG	2 cut(s)	1138, 1247
BfoI	RGCGCY	1 cut(s)	1512
BfuAI	ACCTGC	2 cut(s)	1243, 1250
BfuI	GTATCC	1 cut(s)	348
BisI	GCNGC	7 cut(s)	119, 214, 483, 792, 956, 1170, 1626
BlpI	GCTNAGC	1 cut(s)	516
BlsI	GCNGC	7 cut(s)	120, 215, 484, 793, 957, 1171, 1627
BmcAI	AGTACT	2 cut(s)	188, 311
Bme1390I	CCNGG	3 cut(s)	342, 618, 1318
Bme18I	GGWCC	1 cut(s)	908
BmeT110I	CYCGRG	2 cut(s)	741, 1620
BmgT120I	GGNCC	2 cut(s)	456, 908
BmiI	GGNNCC	2 cut(s)	457, 1684
BmrFI	CCNGG	3 cut(s)	342, 618, 1318
BmsI	GCATC	5 cut(s)	376, 1414, 1511, 1600, 1834
BpiI	GAAGAC	3 cut(s)	605, 1049, 1398
BplI	GAGNNNNNCTC	2 cut(s)	688, 720
BpmI	CTGGAG	1 cut(s)	353
Bpu10I	CCTNAGC	1 cut(s)	1722
Bpu1102I	GCTNAGC	1 cut(s)	516
BpuEI	CTTGAG	1 cut(s)	1079
BsaHI	GRCGYC	1 cut(s)	1109
BsaJI	CCNNGG	2 cut(s)	1159, 1656
BsaWI	WCCGGW	4 cut(s)	73, 159, 1366, 1606
BsaXI	ACNNNNNCTCC	4 cut(s)	258, 288, 795, 825
Bsc4I	CCNNNNNNNGG	3 cut(s)	31, 452, 465
Bse1I	ACTGG	2 cut(s)	130, 1417
Bse3DI	GCAATG	2 cut(s)	484, 1839
BseAI	TCCGGA	1 cut(s)	1606
BseBI	CCWGG	3 cut(s)	342, 618, 1318
BseDI	CCNNGG	2 cut(s)	1159, 1656
BseGI	GGATG	4 cut(s)	751, 1429, 1569, 1615
BseLI	CCNNNNNNNGG	3 cut(s)	31, 452, 465
BseMI	GCAATG	2 cut(s)	484, 1839
BseMII	CTCAG	3 cut(s)	1370, 1742, 1868
BseNI	ACTGG	2 cut(s)	130, 1417
BseRI	GAGGAG	1 cut(s)	561
BseSI	GKGCMC	1 cut(s)	1825
BseXI	GCAGC	7 cut(s)	130, 225, 469, 778, 967, 1156, 1637
BseYI	CCCAGC	1 cut(s)	951
BsgI	GTGCAG	2 cut(s)	269, 1616
Bsh1236I	CGCG	3 cut(s)	102, 690, 1819
BshFI	GGCC	7 cut(s)	58, 143, 182, 458, 642, 828, 1158
BsiHKCI	CYCGRG	2 cut(s)	741, 1620
BsiSI	CCGG	6 cut(s)	74, 144, 160, 179, 1367, 1607
BslFI	GGGAC	3 cut(s)	1244, 1315, 1537
BslI	CCNNNNNNNGG	3 cut(s)	31, 452, 465
BsmAI	GTCTC	1 cut(s)	742
BsmFI	GGGAC	3 cut(s)	1244, 1315, 1537
BsmI	GAATGC	1 cut(s)	992
BsnI	GGCC	7 cut(s)	58, 143, 182, 458, 642, 828, 1158
BsoBI	CYCGRG	2 cut(s)	741, 1620
Bsp1286I	GDGCHC	2 cut(s)	1095, 1825
Bsp13I	TCCGGA	1 cut(s)	1606
Bsp143I	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
Bsp1720I	GCTNAGC	1 cut(s)	516
Bsp19I	CCATGG	1 cut(s)	1159
BspACI	CCGC	6 cut(s)	100, 194, 688, 1180, 1299, 1743
BspANI	GGCC	7 cut(s)	58, 143, 182, 458, 642, 828, 1158
BspCNI	CTCAG	3 cut(s)	1369, 1743, 1867
BspEI	TCCGGA	1 cut(s)	1606
BspFNI	CGCG	3 cut(s)	102, 690, 1819
BspHI	TCATGA	1 cut(s)	1048
BspLI	GGNNCC	2 cut(s)	457, 1684
BspMI	ACCTGC	2 cut(s)	1243, 1250
BspPI	GGATC	5 cut(s)	170, 242, 407, 1400, 1668
BsrDI	GCAATG	2 cut(s)	484, 1839
BsrI	ACTGG	2 cut(s)	130, 1417
BssECI	CCNNGG	2 cut(s)	1159, 1656
BssMI	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
BssNI	GRCGYC	1 cut(s)	1109
BssSI	CACGAG	1 cut(s)	705
BssT1I	CCWWGG	2 cut(s)	1159, 1656
Bst2BI	CACGAG	1 cut(s)	705
Bst2UI	CCWGG	3 cut(s)	342, 618, 1318
Bst4CI	ACNGT	6 cut(s)	156, 257, 508, 1240, 1312, 1604
Bst6I	CTCTTC	1 cut(s)	618
BstACI	GRCGYC	1 cut(s)	1109
BstC8I	GCNNGC	5 cut(s)	104, 865, 978, 1481, 1821
BstDEI	CTNAG	8 cut(s)	283, 291, 516, 1328, 1356, 1722, 1751, 1854
BstDSI	CCRYGG	1 cut(s)	1159
BstF5I	GGATG	4 cut(s)	751, 1429, 1569, 1615
BstFNI	CGCG	3 cut(s)	102, 690, 1819
BstH2I	RGCGCY	1 cut(s)	1512
BstHHI	GCGC	2 cut(s)	692, 1511
BstKTI	GATC	9 cut(s)	97, 178, 237, 415, 420, 1267, 1408, 1648, 1663
BstMAI	GTCTC	1 cut(s)	742
BstMBI	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
BstMWI	GCNNNNNNNGC	3 cut(s)	526, 1249, 1829
BstNI	CCWGG	3 cut(s)	342, 618, 1318
BstNSI	RCATGY	2 cut(s)	226, 1871
BstSCI	CCNGG	3 cut(s)	340, 616, 1316
BstSFI	CTRYAG	2 cut(s)	1138, 1247
BstSLI	GKGCMC	1 cut(s)	1825
BstUI	CGCG	3 cut(s)	102, 690, 1819
BstV1I	GCAGC	7 cut(s)	130, 225, 469, 778, 967, 1156, 1637
BstV2I	GAAGAC	3 cut(s)	605, 1049, 1398
BstX2I	RGATCY	2 cut(s)	175, 1405
BstXI	CCANNNNNNTGG	1 cut(s)	650
BstYI	RGATCY	2 cut(s)	175, 1405
BsuI	GTATCC	1 cut(s)	348
BsuRI	GGCC	7 cut(s)	58, 143, 182, 458, 642, 828, 1158
BtgI	CCRYGG	1 cut(s)	1159
BtsCI	GGATG	4 cut(s)	751, 1429, 1569, 1615
BtsI	GCAGTG	3 cut(s)	699, 853, 1007
BtsIMutI	CAGTG	4 cut(s)	504, 699, 853, 1007
BveI	ACCTGC	2 cut(s)	1243, 1250
Cac8I	GCNNGC	5 cut(s)	104, 865, 978, 1481, 1821
CciI	TCATGA	1 cut(s)	1048
CfoI	GCGC	2 cut(s)	692, 1511
Cfr13I	GGNCC	2 cut(s)	456, 908
CseI	GACGC	3 cut(s)	205, 884, 1799
Csp6I	GTAC	7 cut(s)	163, 187, 253, 310, 581, 1165, 1858
CspCI	CAANNNNNGTGG	2 cut(s)	493, 528
CviAII	CATG	9 cut(s)	223, 371, 491, 536, 1049, 1160, 1205, 1339, 1868
CviQI	GTAC	7 cut(s)	163, 187, 253, 310, 581, 1165, 1858
DdeI	CTNAG	8 cut(s)	283, 291, 516, 1328, 1356, 1722, 1751, 1854
DpnI	GATC	9 cut(s)	96, 177, 236, 414, 419, 1266, 1407, 1647, 1662
DpnII	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
EaeI	YGGCCR	2 cut(s)	180, 640
Eam1104I	CTCTTC	1 cut(s)	618
EarI	CTCTTC	1 cut(s)	618
Eco130I	CCWWGG	2 cut(s)	1159, 1656
Eco147I	AGGCCT	1 cut(s)	58
Eco24I	GRGCYC	1 cut(s)	1095
Eco47I	GGWCC	1 cut(s)	908
Eco57I	CTGAAG	2 cut(s)	50, 825
Eco88I	CYCGRG	2 cut(s)	741, 1620
EcoRII	CCWGG	3 cut(s)	340, 616, 1316
EcoT14I	CCWWGG	2 cut(s)	1159, 1656
EcoT38I	GRGCYC	1 cut(s)	1095
ErhI	CCWWGG	2 cut(s)	1159, 1656
FaeI	CATG	9 cut(s)	226, 374, 494, 539, 1052, 1163, 1208, 1342, 1871
FaqI	GGGAC	3 cut(s)	1244, 1315, 1537
FatI	CATG	9 cut(s)	222, 370, 490, 535, 1048, 1159, 1204, 1338, 1867
FauI	CCCGC	1 cut(s)	1292
FblI	GTMKAC	2 cut(s)	219, 391
Fnu4HI	GCNGC	7 cut(s)	119, 214, 483, 792, 956, 1170, 1626
FokI	GGATG	4 cut(s)	758, 1436, 1556, 1622
FriOI	GRGCYC	1 cut(s)	1095
Fsp4HI	GCNGC	7 cut(s)	119, 214, 483, 792, 956, 1170, 1626
FspBI	CTAG	1 cut(s)	800
GlaI	GCGC	2 cut(s)	691, 1510
GluI	GCNGC	7 cut(s)	119, 214, 483, 792, 956, 1170, 1626
GsaI	CCCAGC	1 cut(s)	955
GsuI	CTGGAG	1 cut(s)	353
HaeII	RGCGCY	1 cut(s)	1512
HaeIII	GGCC	7 cut(s)	58, 143, 182, 458, 642, 828, 1158
HapII	CCGG	6 cut(s)	74, 144, 160, 179, 1367, 1607
HgaI	GACGC	3 cut(s)	205, 884, 1799
HhaI	GCGC	2 cut(s)	692, 1511
Hin1I	GRCGYC	1 cut(s)	1109
Hin1II	CATG	9 cut(s)	226, 374, 494, 539, 1052, 1163, 1208, 1342, 1871
Hin6I	GCGC	2 cut(s)	690, 1509
HinP1I	GCGC	2 cut(s)	690, 1509
HincII	GTYRAC	2 cut(s)	220, 1177
HindII	GTYRAC	2 cut(s)	220, 1177
HindIII	AAGCTT	1 cut(s)	1146
HinfI	GANTC	9 cut(s)	70, 147, 601, 684, 699, 1045, 1334, 1550, 1562
HpaII	CCGG	6 cut(s)	74, 144, 160, 179, 1367, 1607
HphI	GGTGA	4 cut(s)	89, 1274, 1381, 1732
Hpy166II	GTNNAC	6 cut(s)	220, 392, 568, 667, 1177, 1860
Hpy8I	GTNNAC	6 cut(s)	220, 392, 568, 667, 1177, 1860
Hpy99I	CGWCG	3 cut(s)	170, 173, 221
HpyAV	CCTTC	5 cut(s)	74, 82, 583, 850, 1220
HpyCH4III	ACNGT	6 cut(s)	156, 257, 508, 1240, 1312, 1604
HpyCH4IV	ACGT	2 cut(s)	165, 1109
HpyF10VI	GCNNNNNNNGC	3 cut(s)	526, 1249, 1829
HpyF3I	CTNAG	8 cut(s)	283, 291, 516, 1328, 1356, 1722, 1751, 1854
HpySE526I	ACGT	2 cut(s)	165, 1109
Hsp92I	GRCGYC	1 cut(s)	1109
Hsp92II	CATG	9 cut(s)	226, 374, 494, 539, 1052, 1163, 1208, 1342, 1871
HspAI	GCGC	2 cut(s)	690, 1509
Kpn2I	TCCGGA	1 cut(s)	1606
Kzo9I	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
LmnI	GCTCC	1 cut(s)	674
Lsp1109I	GCAGC	7 cut(s)	130, 225, 469, 778, 967, 1156, 1637
LweI	GCATC	5 cut(s)	376, 1414, 1511, 1600, 1834
MaeI	CTAG	1 cut(s)	800
MaeII	ACGT	2 cut(s)	165, 1109
MaeIII	GTNAC	4 cut(s)	1105, 1312, 1369, 1813
MalI	GATC	9 cut(s)	96, 177, 236, 414, 419, 1266, 1407, 1647, 1662
MboI	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
MboII	GAAGA	8 cut(s)	185, 605, 610, 707, 831, 1051, 1054, 1403
MfeI	CAATTG	3 cut(s)	870, 1058, 1827
MflI	RGATCY	2 cut(s)	175, 1405
MhlI	GDGCHC	2 cut(s)	1095, 1825
MlsI	TGGCCA	1 cut(s)	642
MluCI	AATT	5 cut(s)	435, 621, 870, 1058, 1827
MluI	ACGCGT	1 cut(s)	1817
MluNI	TGGCCA	1 cut(s)	642
MlyI	GAGTC	4 cut(s)	64, 595, 1039, 1559
MmeI	TCCRAC	1 cut(s)	1401
MnlI	CCTC	9 cut(s)	108, 133, 260, 539, 714, 813, 921, 1148, 1188
Mox20I	TGGCCA	1 cut(s)	642
MroI	TCCGGA	1 cut(s)	1606
MscI	TGGCCA	1 cut(s)	642
MseI	TTAA	5 cut(s)	680, 720, 965, 1526, 1879
Msp20I	TGGCCA	1 cut(s)	642
MspI	CCGG	6 cut(s)	74, 144, 160, 179, 1367, 1607
MspR9I	CCNGG	3 cut(s)	342, 618, 1318
MunI	CAATTG	3 cut(s)	870, 1058, 1827
Mva1269I	GAATGC	1 cut(s)	992
MvaI	CCWGG	3 cut(s)	342, 618, 1318
MvnI	CGCG	3 cut(s)	102, 690, 1819
MwoI	GCNNNNNNNGC	3 cut(s)	526, 1249, 1829
NcoI	CCATGG	1 cut(s)	1159
NdeII	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
NlaIII	CATG	9 cut(s)	226, 374, 494, 539, 1052, 1163, 1208, 1342, 1871
NlaIV	GGNNCC	2 cut(s)	457, 1684
NmuCI	GTSAC	2 cut(s)	1105, 1369
NspI	RCATGY	2 cut(s)	226, 1871
PaeR7I	CTCGAG	1 cut(s)	1620
PagI	TCATGA	1 cut(s)	1048
PaqCI	CACCTGC	1 cut(s)	1250
PceI	AGGCCT	1 cut(s)	58
PciI	ACATGT	1 cut(s)	222
PcsI	WCGNNNNNNNCGW	1 cut(s)	99
PctI	GAATGC	1 cut(s)	992
PfeI	GAWTC	5 cut(s)	147, 684, 699, 1334, 1562
PflMI	CCANNNNNTGG	2 cut(s)	452, 465
PfoI	TCCNGGA	1 cut(s)	616
PkrI	GCNGC	7 cut(s)	120, 215, 484, 793, 957, 1171, 1627
PleI	GAGTC	4 cut(s)	64, 595, 1039, 1558
PpsI	GAGTC	4 cut(s)	64, 595, 1039, 1558
PscI	ACATGT	1 cut(s)	222
Psp6I	CCWGG	3 cut(s)	340, 616, 1316
PspFI	CCCAGC	1 cut(s)	951
PspGI	CCWGG	3 cut(s)	340, 616, 1316
PspN4I	GGNNCC	2 cut(s)	457, 1684
PspPI	GGNCC	2 cut(s)	456, 908
PsuI	RGATCY	2 cut(s)	175, 1405
RsaI	GTAC	7 cut(s)	164, 188, 254, 311, 582, 1166, 1859
RsaNI	GTAC	7 cut(s)	163, 187, 253, 310, 581, 1165, 1858
SalI	GTCGAC	1 cut(s)	218
SaqAI	TTAA	5 cut(s)	680, 720, 965, 1526, 1879
SatI	GCNGC	7 cut(s)	119, 214, 483, 792, 956, 1170, 1626
Sau3AI	GATC	9 cut(s)	94, 175, 234, 412, 417, 1264, 1405, 1645, 1660
Sau96I	GGNCC	2 cut(s)	456, 908
ScaI	AGTACT	2 cut(s)	188, 311
SchI	GAGTC	4 cut(s)	64, 595, 1039, 1559
ScrFI	CCNGG	3 cut(s)	342, 618, 1318
SduI	GDGCHC	2 cut(s)	1095, 1825
SfaNI	GCATC	5 cut(s)	376, 1414, 1511, 1600, 1834
SfcI	CTRYAG	2 cut(s)	1138, 1247
Sfr274I	CTCGAG	1 cut(s)	1620
SinI	GGWCC	1 cut(s)	908
SlaI	CTCGAG	1 cut(s)	1620
SmlI	CTYRAG	2 cut(s)	1094, 1620
SmoI	CTYRAG	2 cut(s)	1094, 1620
Sse9I	AATT	5 cut(s)	435, 621, 870, 1058, 1827
SseBI	AGGCCT	1 cut(s)	58
SsiI	CCGC	6 cut(s)	100, 194, 688, 1180, 1299, 1743
SspI	AATATT	1 cut(s)	1028
SspMI	CTAG	1 cut(s)	800
StuI	AGGCCT	1 cut(s)	58
StyD4I	CCNGG	3 cut(s)	340, 616, 1316
StyI	CCWWGG	2 cut(s)	1159, 1656
TaaI	ACNGT	6 cut(s)	156, 257, 508, 1240, 1312, 1604
TaiI	ACGT	2 cut(s)	168, 1112
TaqI	TCGA	6 cut(s)	171, 219, 1378, 1447, 1621, 1663
TasI	AATT	5 cut(s)	435, 621, 870, 1058, 1827
TatI	WGTACW	4 cut(s)	186, 252, 309, 1857
TfiI	GAWTC	5 cut(s)	147, 684, 699, 1334, 1562
Tru1I	TTAA	5 cut(s)	680, 720, 965, 1526, 1879
Tru9I	TTAA	5 cut(s)	680, 720, 965, 1526, 1879
TscAI	CASTG	4 cut(s)	511, 699, 853, 1014
TseFI	GTSAC	2 cut(s)	1105, 1369
TseI	GCWGC	7 cut(s)	118, 213, 482, 791, 955, 1169, 1625
Tsp45I	GTSAC	2 cut(s)	1105, 1369
TspDTI	ATGAA	6 cut(s)	479, 565, 611, 1052, 1068, 1355
TspRI	CASTG	4 cut(s)	511, 699, 853, 1014
Van91I	CCANNNNNTGG	2 cut(s)	452, 465
VpaK11BI	GGWCC	1 cut(s)	908
XceI	RCATGY	2 cut(s)	226, 1871
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1012
XhoI	CTCGAG	1 cut(s)	1620
XmiI	GTMKAC	2 cut(s)	219, 391
XspI	CTAG	1 cut(s)	800
ZraI	GACGTC	1 cut(s)	1110
ZrmI	AGTACT	2 cut(s)	188, 311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.