Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008297

Physical Location & Seq

Genomic Coordinates Reverse (-)

14678756 .. 14682303

Gene Structure 3548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1046000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGAAAATGGAGCTGACAGCCATTAAAGATGCATTTGATCGTGTTACAAAGAAACAAAAGCTGTCAAACTCTAAAGTTCAAGAAGTGTTCGATCTGATCGACAAAGAAATCAAGCAGACATTACAGAAGCTACAATCATCTAATGATCGTGAATCTCGGATTGATTGTAAGTCTGTTCTTGCTGATCTTAAAGCCAAGTTAAAAGATATAGCTCCACTCAGTCAGTTGGAAAGCACGCAGAAGGAACTGAACCTAGCACTTAGCAAGTATCCAAAGATTGTGGAGAAATCCTTCGTTCCTGATATATCCAAGGCTTATAGAAACATTGACTTTGATACCCACACAGTCAATGAGATAATTGGCAGCCATTTCTATCGGCAGGGCCTATTCGAAGTTGGTGATTGTTTTACAAGTGAGACTGGGGAATCAGTATCTGCATTGGCTATGAAATCTCAGTTTCAGGAATTGTTTGGGATAGTTGATGCAATGAAACATCGGAATCTAGAGCCAGCACTGAACTGGGCCATGTTTAACTCTGATAAACTCAAACCAAATGGATCAGACCTATTACTGAAACTTCACAGACTGCAATTTGTTGAAATAGTGCAAAAGGGAAATAGAGATAGAGCTCTCCAGTATTCAAAAGCTTGTCTTGCTCCGTTTGCTTCCAACCATATGGTTGAAATACAAAAGCTTATGGGGGCTCTTCTGTGGACTGGAAGGCTTGAGCACTCCCCATATTCTCACTTATTATCACCAGCCAATTGGGACACAGTGACGGGGGAGCTAACCAGGCTGTTCTGCAATATTCTGGGTCAGTCTTACAAGAGCCCGTTAAGTGTGACCATGGAGGCAGGGATTCGTGGTTTGCCACCTCTTCTCAAGTTTATGAACGTAATGGCAGGAAAGAGATTGGAATGGCAGTCTATGAAGCAGTTACCTGTACCAGTGGAGTTAGACCGTGAGTTTCATTTCCATTCTATATTTGTGTGTCCAGTATCAAAGGAACAATCAACAGAAGAAAATCCGCCTATGTTGATGTCATGTGGACATGTGCTCTGCAAGCAGTCTATTACGAAGATGTCAAAGAACGGTACAAAATCTTTCAAGTGTCCATACTGTCCTTCCGATGTCAATTCCGCACAGTGTAGGCCGCTTGTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0034657 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLTH zf-RING_UBOX

Protein Analysis

389

Amino Acids

43.98

Weight (kDa)

8.85

Isoelectric Point (pI)

41.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CTLH	PF10607	152 - 291	1.6e-33	CTLH/CRA C-terminal to LisH motif domain
zf-RING_UBOX	PF13445	331 - 372	3.9e-10	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008297)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22690 AT2G22690 AT4G37880
fragaria_vesca	FvH4_1g18890 FvH4_1g18890 FvH4_1g18890
malus_domestica	MD01G1046000.v1.1 MD01G1046100.v1.1 MD15G1305600.v1.1
prunus_persica	Prupe.6G210700_v2.0.a1
pyrus_communis	pycom01g07220 pycom15g26920 pycom15g26940
rosa_laevigata	RLG00000017766
rosa_multiflora	Rmu_sc0008299.1_g000002
rosa_roxburghii	Rroxscaffold_2G00133920
rosa_rugosa	Rorug02G0163000 Rorug02G0163100
rosa_samantha	Rh2AG215200 Rh2BG225300 Rh2CG217100 Rh2DG220700
rosa_wichuraiana	Rw2G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	1028, 1140, 1154
AclWI	GGATC	1 cut(s)	565
AfaI	GTAC	2 cut(s)	945, 1096
AflIII	ACRYGT	1 cut(s)	1051
AgsI	TTSAA	5 cut(s)	80, 599, 642, 683, 1108
AjnI	CCWGG	1 cut(s)	791
AluBI	AGCT	8 cut(s)	13, 61, 130, 212, 629, 647, 694, 787
AluI	AGCT	8 cut(s)	13, 61, 130, 212, 629, 647, 694, 787
Alw21I	GWGCWC	3 cut(s)	631, 732, 1059
Alw26I	GTCTC	1 cut(s)	410
AlwI	GGATC	1 cut(s)	565
AlwNI	CAGNNNCTG	1 cut(s)	434
AoxI	GGCC	3 cut(s)	382, 522, 1151
ApeKI	GCWGC	1 cut(s)	363
Asp700I	GAANNNNTTC	1 cut(s)	290
AspS9I	GGNCC	2 cut(s)	382, 522
AsuHPI	GGTGA	2 cut(s)	410, 747
AsuII	TTCGAA	1 cut(s)	390
BanII	GRGCYC	3 cut(s)	631, 706, 833
Bbv12I	GWGCWC	3 cut(s)	631, 732, 1059
BbvI	GCAGC	1 cut(s)	375
BciT130I	CCWGG	1 cut(s)	793
BciVI	GTATCC	1 cut(s)	279
BcoDI	GTCTC	1 cut(s)	410
BfaI	CTAG	2 cut(s)	254, 503
BfuI	GTATCC	1 cut(s)	279
BisI	GCNGC	2 cut(s)	364, 1154
BlsI	GCNGC	2 cut(s)	365, 1155
Bme1390I	CCNGG	1 cut(s)	793
BmgT120I	GGNCC	2 cut(s)	382, 522
BmrFI	CCNGG	1 cut(s)	793
BmrI	ACTGGG	2 cut(s)	429, 529
BmsI	GCATC	2 cut(s)	19, 472
BmuI	ACTGGG	2 cut(s)	429, 529
BpmI	CTGGAG	1 cut(s)	617
Bpu14I	TTCGAA	1 cut(s)	390
BpuEI	CTTGAG	2 cut(s)	746, 866
BsaJI	CCNNGG	2 cut(s)	309, 846
Bse1I	ACTGG	6 cut(s)	424, 524, 634, 721, 947, 995
Bse3DI	GCAATG	1 cut(s)	492
BseBI	CCWGG	1 cut(s)	793
BseDI	CCNNGG	2 cut(s)	309, 846
BseMI	GCAATG	1 cut(s)	492
BseMII	CTCAG	2 cut(s)	232, 467
BseNI	ACTGG	6 cut(s)	424, 524, 634, 721, 947, 995
BseXI	GCAGC	1 cut(s)	375
BshFI	GGCC	3 cut(s)	384, 524, 1153
BsiHKAI	GWGCWC	3 cut(s)	631, 732, 1059
BslFI	GGGAC	1 cut(s)	782
BsmAI	GTCTC	1 cut(s)	410
BsmFI	GGGAC	1 cut(s)	782
BsnI	GGCC	3 cut(s)	384, 524, 1153
Bsp119I	TTCGAA	1 cut(s)	390
Bsp1286I	GDGCHC	5 cut(s)	631, 706, 732, 833, 1059
Bsp143I	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
Bsp19I	CCATGG	1 cut(s)	846
BspACI	CCGC	3 cut(s)	1028, 1140, 1154
BspANI	GGCC	3 cut(s)	384, 524, 1153
BspCNI	CTCAG	2 cut(s)	231, 466
BspPI	GGATC	1 cut(s)	565
BspQI	GCTCTTC	1 cut(s)	711
BspT104I	TTCGAA	1 cut(s)	390
BsrDI	GCAATG	1 cut(s)	492
BsrI	ACTGG	6 cut(s)	424, 524, 634, 721, 947, 995
BssECI	CCNNGG	2 cut(s)	309, 846
BssMI	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
BssT1I	CCWWGG	2 cut(s)	309, 846
Bst2UI	CCWGG	1 cut(s)	793
Bst4CI	ACNGT	6 cut(s)	346, 775, 962, 1094, 1121, 1146
Bst6I	CTCTTC	2 cut(s)	711, 882
BstBI	TTCGAA	1 cut(s)	390
BstC8I	GCNNGC	3 cut(s)	236, 510, 1064
BstDEI	CTNAG	3 cut(s)	218, 260, 453
BstDSI	CCRYGG	1 cut(s)	846
BstKTI	GATC	6 cut(s)	40, 94, 99, 148, 187, 560
BstMAI	GTCTC	1 cut(s)	410
BstMBI	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
BstMWI	GCNNNNNNNGC	4 cut(s)	653, 662, 793, 1063
BstNI	CCWGG	1 cut(s)	793
BstNSI	RCATGY	1 cut(s)	1055
BstSCI	CCNGG	1 cut(s)	791
BstV1I	GCAGC	1 cut(s)	375
BstXI	CCANNNNNNTGG	2 cut(s)	676, 765
BsuI	GTATCC	1 cut(s)	279
BsuRI	GGCC	3 cut(s)	384, 524, 1153
BtgI	CCRYGG	1 cut(s)	846
BtsIMutI	CAGTG	4 cut(s)	512, 780, 954, 1151
Cac8I	GCNNGC	3 cut(s)	236, 510, 1064
CaiI	CAGNNNCTG	1 cut(s)	434
Cfr13I	GGNCC	2 cut(s)	382, 522
Csp6I	GTAC	2 cut(s)	944, 1095
CspCI	CAANNNNNGTGG	2 cut(s)	261, 296
CviAII	CATG	4 cut(s)	526, 847, 1044, 1052
CviQI	GTAC	2 cut(s)	944, 1095
DdeI	CTNAG	3 cut(s)	218, 260, 453
DpnI	GATC	6 cut(s)	39, 93, 98, 147, 186, 559
DpnII	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
Eam1104I	CTCTTC	2 cut(s)	711, 882
EarI	CTCTTC	2 cut(s)	711, 882
EciI	GGCGGA	1 cut(s)	1017
Ecl136II	GAGCTC	1 cut(s)	629
Eco130I	CCWWGG	2 cut(s)	309, 846
Eco24I	GRGCYC	3 cut(s)	631, 706, 833
Eco53kI	GAGCTC	1 cut(s)	629
EcoICRI	GAGCTC	1 cut(s)	629
EcoO109I	RGGNCCY	1 cut(s)	382
EcoRII	CCWGG	1 cut(s)	791
EcoT14I	CCWWGG	2 cut(s)	309, 846
EcoT22I	ATGCAT	1 cut(s)	34
EcoT38I	GRGCYC	3 cut(s)	631, 706, 833
ErhI	CCWWGG	2 cut(s)	309, 846
FaeI	CATG	4 cut(s)	529, 850, 1047, 1055
FalI	AAGNNNNNCTT	2 cut(s)	636, 668
FaqI	GGGAC	1 cut(s)	782
FatI	CATG	4 cut(s)	525, 846, 1043, 1051
FauNDI	CATATG	1 cut(s)	675
Fnu4HI	GCNGC	2 cut(s)	364, 1154
FriOI	GRGCYC	3 cut(s)	631, 706, 833
Fsp4HI	GCNGC	2 cut(s)	364, 1154
FspBI	CTAG	2 cut(s)	254, 503
GluI	GCNGC	2 cut(s)	364, 1154
GsuI	CTGGAG	1 cut(s)	617
HaeIII	GGCC	3 cut(s)	384, 524, 1153
Hin1II	CATG	4 cut(s)	529, 850, 1047, 1055
HindIII	AAGCTT	2 cut(s)	645, 692
HinfI	GANTC	4 cut(s)	152, 425, 499, 859
HphI	GGTGA	2 cut(s)	410, 747
Hpy166II	GTNNAC	2 cut(s)	714, 1049
Hpy188I	TCNGA	7 cut(s)	96, 159, 498, 538, 562, 1129, 1166
Hpy188III	TCNNGA	5 cut(s)	80, 149, 299, 461, 503
Hpy8I	GTNNAC	2 cut(s)	714, 1049
HpyAV	CCTTC	4 cut(s)	235, 301, 714, 1134
HpyCH4III	ACNGT	6 cut(s)	346, 775, 962, 1094, 1121, 1146
HpyCH4IV	ACGT	1 cut(s)	894
HpyCH4V	TGCA	7 cut(s)	32, 437, 485, 589, 607, 804, 1062
HpyF10VI	GCNNNNNNNGC	4 cut(s)	653, 662, 793, 1063
HpyF3I	CTNAG	3 cut(s)	218, 260, 453
HpySE526I	ACGT	1 cut(s)	894
Hsp92II	CATG	4 cut(s)	529, 850, 1047, 1055
Kzo9I	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
LguI	GCTCTTC	1 cut(s)	711
LmnI	GCTCC	4 cut(s)	10, 217, 661, 784
Lsp1109I	GCAGC	1 cut(s)	375
LweI	GCATC	2 cut(s)	19, 472
MaeI	CTAG	2 cut(s)	254, 503
MaeII	ACGT	1 cut(s)	894
MaeIII	GTNAC	4 cut(s)	43, 775, 841, 936
MalI	GATC	6 cut(s)	39, 93, 98, 147, 186, 559
MboI	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
MboII	GAAGA	4 cut(s)	698, 869, 1031, 1090
MfeI	CAATTG	1 cut(s)	763
MhlI	GDGCHC	5 cut(s)	631, 706, 732, 833, 1059
MluCI	AATT	5 cut(s)	357, 464, 590, 763, 1135
MmeI	TCCRAC	2 cut(s)	207, 693
MnlI	CCTC	2 cut(s)	844, 885
Mph1103I	ATGCAT	1 cut(s)	34
MroXI	GAANNNNTTC	1 cut(s)	290
MseI	TTAA	5 cut(s)	24, 189, 200, 531, 836
MspR9I	CCNGG	1 cut(s)	793
MunI	CAATTG	1 cut(s)	763
MvaI	CCWGG	1 cut(s)	793
MwoI	GCNNNNNNNGC	4 cut(s)	653, 662, 793, 1063
NcoI	CCATGG	1 cut(s)	846
NdeI	CATATG	1 cut(s)	675
NdeII	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
NlaIII	CATG	4 cut(s)	529, 850, 1047, 1055
NmuCI	GTSAC	2 cut(s)	775, 841
NsiI	ATGCAT	1 cut(s)	34
NspI	RCATGY	1 cut(s)	1055
NspV	TTCGAA	1 cut(s)	390
PciI	ACATGT	1 cut(s)	1051
PciSI	GCTCTTC	1 cut(s)	711
PcsI	WCGNNNNNNNCGW	1 cut(s)	96
PdmI	GAANNNNTTC	1 cut(s)	290
PfeI	GAWTC	4 cut(s)	152, 425, 499, 859
PkrI	GCNGC	2 cut(s)	365, 1155
PscI	ACATGT	1 cut(s)	1051
Psp124BI	GAGCTC	1 cut(s)	631
Psp6I	CCWGG	1 cut(s)	791
PspGI	CCWGG	1 cut(s)	791
PspPI	GGNCC	2 cut(s)	382, 522
PstNI	CAGNNNCTG	1 cut(s)	434
RsaI	GTAC	2 cut(s)	945, 1096
RsaNI	GTAC	2 cut(s)	944, 1095
SacI	GAGCTC	1 cut(s)	631
SapI	GCTCTTC	1 cut(s)	711
SaqAI	TTAA	5 cut(s)	24, 189, 200, 531, 836
SatI	GCNGC	2 cut(s)	364, 1154
Sau3AI	GATC	6 cut(s)	37, 91, 96, 145, 184, 557
Sau96I	GGNCC	2 cut(s)	382, 522
ScrFI	CCNGG	1 cut(s)	793
SduI	GDGCHC	5 cut(s)	631, 706, 732, 833, 1059
SfaNI	GCATC	2 cut(s)	19, 472
SfuI	TTCGAA	1 cut(s)	390
SmlI	CTYRAG	2 cut(s)	725, 881
SmoI	CTYRAG	2 cut(s)	725, 881
Sse9I	AATT	5 cut(s)	357, 464, 590, 763, 1135
SsiI	CCGC	3 cut(s)	1028, 1140, 1154
SspI	AATATT	1 cut(s)	808
SspMI	CTAG	2 cut(s)	254, 503
SstI	GAGCTC	1 cut(s)	631
StyD4I	CCNGG	1 cut(s)	791
StyI	CCWWGG	2 cut(s)	309, 846
TaaI	ACNGT	6 cut(s)	346, 775, 962, 1094, 1121, 1146
TaiI	ACGT	1 cut(s)	897
TaqI	TCGA	3 cut(s)	90, 99, 390
TasI	AATT	5 cut(s)	357, 464, 590, 763, 1135
TauI	GCSGC	1 cut(s)	1156
TfiI	GAWTC	4 cut(s)	152, 425, 499, 859
Tru1I	TTAA	5 cut(s)	24, 189, 200, 531, 836
Tru9I	TTAA	5 cut(s)	24, 189, 200, 531, 836
TscAI	CASTG	4 cut(s)	519, 780, 954, 1151
TseFI	GTSAC	2 cut(s)	775, 841
TseI	GCWGC	1 cut(s)	363
Tsp45I	GTSAC	2 cut(s)	775, 841
TspDTI	ATGAA	6 cut(s)	17, 461, 503, 905, 944, 959
TspGWI	ACGGA	1 cut(s)	648
TspRI	CASTG	4 cut(s)	519, 780, 954, 1151
XbaI	TCTAGA	1 cut(s)	502
XceI	RCATGY	1 cut(s)	1055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	516
XmnI	GAANNNNTTC	1 cut(s)	290
XspI	CTAG	2 cut(s)	254, 503
Zsp2I	ATGCAT	1 cut(s)	34

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.